POLR1E cDNA ORF clone, Tinamus guttatus(white-throated tinamou)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR1E
Entrez Gene ID	104573463
Full Name	RNA polymerase I subunit E
Gene Type	protein-coding
Organism	Tinamus guttatus(white-throated tinamou)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010220661.1	XP_010218963.1	DNA-directed RNA polymerase I subunit RPA49

Pathways from BioSystems
Gene Ontology

The following POLR1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi21654	XM_010220661.1 Latest version!	Tinamus guttatus polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR1E ( XM_010220661.1 ) cDNA ORF clone, Tinamus guttatus(white-throated tinamou) -> XP_010218963.1 Tinamus guttatus polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.

Price & Availability↑

CloneID	OTi21654
Clone ID Related Accession (Same CDS sequence)	XM_010220661.1
Accession Version	XM_010220661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-12
Organism	Tinamus guttatus(white-throated tinamou)
Product	DNA-directed RNA polymerase I subunit RPA49
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010585731.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tinamus guttatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGAACGAAG CAATCTCCAC AAACCCTGTG CTGGTTTTAG GCAGGGACGA GGACCTGCGC 
TTCAGCGTGT ACCGGGACGT GGAGCGCACC CAGCCGCGCA GGAGGAACCG CAGGCTCCTG 
GTCTCAGAAA CCGAAAGGCT TTCCTATGTG GGGAATAACT TTGGCAGTGG TGTTATGAAG 
TGCAATTCCC TTTGCAGGTA CTTCATTGGT GTGTTAGACA AGGATACCGG GCAAATGGAC 
GTCTACAATG CTGAATTGTT CAACATGCAG CCATGGTTGT CAGATAACTT AGTAGCTGAT 
GAAAGAGAGG ATTATCAGAA TAAGTCCTAC AGGGAGAAGA TGGATTTGTG CATTGAGGCT 
TTTGGTACCA GCAAGCAGAA GCGAGCCCTG AGCTCTCGGC GAATGAATGC AGTGGGGAGT 
GACATCCTGA GCACAGCAGT CACAAAAGCA GCAGCAAACA TTATAGACAC AAAGGGAATA 
ACTGCTTTGA TCGAGGATGT GGCCCAAGAT GACGTACAGA ACATTTCTAT CTTCCTGCCT 
CCATGTCATG AAGATGCTGA CAAACCAGAA GATGTCTACA AATTTGAGGA CATTCTCTCC 
CCTGCAGAGT ATGAAGCGCT GCGGGTTCCA GCAGAAGTCT TCATTAACAT AACTCCAGAG 
GAAATCATTA AGAAAACAGA AGAGAAAAGC TATTGCTCCT TTGTTCTGGA GGAGCTGAAG 
TTCTTGCCTC CTGATGAGAA GAGCAGGGAT CACAAAGCCC GATGCCTCTG GTTCCTAGAT 
ATTCTTATTA AGTTCAGCTT CCTGAAAGTG ATCAAGAAGA AACATCCAAT GGGCCCTGAA 
TGTCCACACA TCATTAGCAG GAAACTCATG AAGAATTTCA CTCTACTGAC CTATAACAAT 
GGCAGCATCC AGAATTTAAT CTCTGCATCC ATGAAGGCTA AAATCACAGC ATACGTCATT 
GCACTGGCTC TGCACATTAA CAACTTCCAA ACAGACCTTA CTGTCCTGCA AAATGACATG 
AAGCTTCAAG AAAGCAGGAT GATGGACATT GCCAAGGCCA TGCGGTTAAA GGTCTCTAAA 
ACAAAGGGGC CTCCAGAACT TGAGCCGGAC CAGAACCACA AGCTGGCAAC TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010218963.1
CDS	1..1137
Translation	MNEAISTNPVLVLGRDEDLRFSVYRDVERTQPRRRNRRLLVSETERLSYVGNNFGSGVMKCNSLCRYFIGVLDKDTGQMDVYNAELFNMQPWLSDNLVADEREDYQNKSYREKMDLCIEAFGTSKQKRALSSRRMNAVGSDILSTAVTKAAANIIDTKGITALIEDVAQDDVQNISIFLPPCHEDADKPEDVYKFEDILSPAEYEALRVPAEVFINITPEEIIKKTEEKSYCSFVLEELKFLPPDEKSRDHKARCLWFLDILIKFSFLKVIKKKHPMGPECPHIISRKLMKNFTLLTYNNGSIQNLISASMKAKITAYVIALALHINNFQTDLTVLQNDMKLQESRMMDIAKAMRLKVSKTKGPPELEPDQNHKLATL

Target ORF information:

RefSeq Version	XM_010220661.1
Organism	Tinamus guttatus(white-throated tinamou)
Definition	Tinamus guttatus polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010220661.1

ORF Insert Sequence:

ATGAACGAAG CAATCTCCAC AAACCCTGTG CTGGTTTTAG GCAGGGACGA GGACCTGCGC 
TTCAGCGTGT ACCGGGACGT GGAGCGCACC CAGCCGCGCA GGAGGAACCG CAGGCTCCTG 
GTCTCAGAAA CCGAAAGGCT TTCCTATGTG GGGAATAACT TTGGCAGTGG TGTTATGAAG 
TGCAATTCCC TTTGCAGGTA CTTCATTGGT GTGTTAGACA AGGATACCGG GCAAATGGAC 
GTCTACAATG CTGAATTGTT CAACATGCAG CCATGGTTGT CAGATAACTT AGTAGCTGAT 
GAAAGAGAGG ATTATCAGAA TAAGTCCTAC AGGGAGAAGA TGGATTTGTG CATTGAGGCT 
TTTGGTACCA GCAAGCAGAA GCGAGCCCTG AGCTCTCGGC GAATGAATGC AGTGGGGAGT 
GACATCCTGA GCACAGCAGT CACAAAAGCA GCAGCAAACA TTATAGACAC AAAGGGAATA 
ACTGCTTTGA TCGAGGATGT GGCCCAAGAT GACGTACAGA ACATTTCTAT CTTCCTGCCT 
CCATGTCATG AAGATGCTGA CAAACCAGAA GATGTCTACA AATTTGAGGA CATTCTCTCC 
CCTGCAGAGT ATGAAGCGCT GCGGGTTCCA GCAGAAGTCT TCATTAACAT AACTCCAGAG 
GAAATCATTA AGAAAACAGA AGAGAAAAGC TATTGCTCCT TTGTTCTGGA GGAGCTGAAG 
TTCTTGCCTC CTGATGAGAA GAGCAGGGAT CACAAAGCCC GATGCCTCTG GTTCCTAGAT 
ATTCTTATTA AGTTCAGCTT CCTGAAAGTG ATCAAGAAGA AACATCCAAT GGGCCCTGAA 
TGTCCACACA TCATTAGCAG GAAACTCATG AAGAATTTCA CTCTACTGAC CTATAACAAT 
GGCAGCATCC AGAATTTAAT CTCTGCATCC ATGAAGGCTA AAATCACAGC ATACGTCATT 
GCACTGGCTC TGCACATTAA CAACTTCCAA ACAGACCTTA CTGTCCTGCA AAATGACATG 
AAGCTTCAAG AAAGCAGGAT GATGGACATT GCCAAGGCCA TGCGGTTAAA GGTCTCTAAA 
ACAAAGGGGC CTCCAGAACT TGAGCCGGAC CAGAACCACA AGCTGGCAAC TCTTTAA