NIT2 cDNA ORF clone, Tinamus guttatus(white-throated tinamou)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIT2
Entrez Gene ID	104567030
Full Name	nitrilase family member 2
Gene Type	protein-coding
Organism	Tinamus guttatus(white-throated tinamou)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010213831.1	XP_010212133.1	omega-amidase NIT2

Pathways from BioSystems
Gene Ontology

The following NIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi16900	XM_010213831.1 Latest version!	Tinamus guttatus nitrilase family, member 2 (NIT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIT2 ( XM_010213831.1 ) cDNA ORF clone, Tinamus guttatus(white-throated tinamou) -> XP_010212133.1 Tinamus guttatus nitrilase family, member 2 (NIT2), mRNA.

Price & Availability↑

CloneID	OTi16900
Clone ID Related Accession (Same CDS sequence)	XM_010213831.1
Accession Version	XM_010213831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-12
Organism	Tinamus guttatus(white-throated tinamou)
Product	omega-amidase NIT2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010580915.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tinamus guttatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGTGCCTCA TAACCAAGGC AAATGAATTT TGTTCCACAC CTACTGATGA ATTCAAATTT 
TGCTGTGTTT TACCTGGGCG ATTTCACTGG CCAGTTGTTT CTTTTCTGTC ACTAGACTTT 
CGTCTGGCGC TCATTCAGCT TCAAGTATCC ACCGTCAAAT CCGACAACCT GCAGCGAGCC 
TGCAGGATGG TGAAAGAAGC ATCAGCAAAA GGAGCTCAAG TTGTGGCTCT GCCTGAATGT 
TTTAACTCTC CTTATGGAAC GCACTTCTTC AAGGAATATG CAGAGAACAT CCCTGGAGAA 
ACGACACACA AACTCTCAGA AACCGCAAAG GAGTGTAACG TATATCTTGT TGGAGGATCC 
ATTCCGGAAG AGGATGCTGG AAAGCTGTAT AATACCTGTA CTGTCTTTGG GCCTGATGGA 
GCTATGTTGA CCAAGTATAG GAAGATTCAT TTGTTTGACA TTAATGTTCC TGGGAAGATA 
CAGTTCAAAG AGTCGGAAAC ACTGAGTCCA GGGAACACTT TCTCTACATT TGATACTCCG 
TACTGCAGAG TGGGCCTGGG CATCTGCTAC GACATAAGGT TTGCTGAGCT GGCTCAGATC 
TACGGGCAGA AAGGTTGCCA GCTGTTGGTG TATCCGGGAG CTTTTAACCT GACAACAGGA 
CCAGCGCACT GGGAACTACT ACAAAGGGGA CGAGCTCTTG ATAATCAAGT CTATGTAGCC 
ACTGCCTCTC CTGCTAGAGA TGAAAATGCT TCCTACATTG CTTGGGGACA CAGCACTGTA 
GTAAATCCAT GGGGGGAAGT TATTGCCAAA GCTGGTGCCG AGGAGGCCAT TCTGTACGCA 
GATATAGATC TGAAGAAGCT TGCGGAAATC CGTGAGCAAA TCCCGGTTTT GAACCAGAAG 
CGCAACGATC TGTATGGCCT GGAGGTGAAA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010212133.1
CDS	1..936
Translation	MCLITKANEFCSTPTDEFKFCCVLPGRFHWPVVSFLSLDFRLALIQLQVSTVKSDNLQRACRMVKEASAKGAQVVALPECFNSPYGTHFFKEYAENIPGETTHKLSETAKECNVYLVGGSIPEEDAGKLYNTCTVFGPDGAMLTKYRKIHLFDINVPGKIQFKESETLSPGNTFSTFDTPYCRVGLGICYDIRFAELAQIYGQKGCQLLVYPGAFNLTTGPAHWELLQRGRALDNQVYVATASPARDENASYIAWGHSTVVNPWGEVIAKAGAEEAILYADIDLKKLAEIREQIPVLNQKRNDLYGLEVKK

Target ORF information:

RefSeq Version	XM_010213831.1
Organism	Tinamus guttatus(white-throated tinamou)
Definition	Tinamus guttatus nitrilase family, member 2 (NIT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010213831.1

ORF Insert Sequence:

ATGTGCCTCA TAACCAAGGC AAATGAATTT TGTTCCACAC CTACTGATGA ATTCAAATTT 
TGCTGTGTTT TACCTGGGCG ATTTCACTGG CCAGTTGTTT CTTTTCTGTC ACTAGACTTT 
CGTCTGGCGC TCATTCAGCT TCAAGTATCC ACCGTCAAAT CCGACAACCT GCAGCGAGCC 
TGCAGGATGG TGAAAGAAGC ATCAGCAAAA GGAGCTCAAG TTGTGGCTCT GCCTGAATGT 
TTTAACTCTC CTTATGGAAC GCACTTCTTC AAGGAATATG CAGAGAACAT CCCTGGAGAA 
ACGACACACA AACTCTCAGA AACCGCAAAG GAGTGTAACG TATATCTTGT TGGAGGATCC 
ATTCCGGAAG AGGATGCTGG AAAGCTGTAT AATACCTGTA CTGTCTTTGG GCCTGATGGA 
GCTATGTTGA CCAAGTATAG GAAGATTCAT TTGTTTGACA TTAATGTTCC TGGGAAGATA 
CAGTTCAAAG AGTCGGAAAC ACTGAGTCCA GGGAACACTT TCTCTACATT TGATACTCCG 
TACTGCAGAG TGGGCCTGGG CATCTGCTAC GACATAAGGT TTGCTGAGCT GGCTCAGATC 
TACGGGCAGA AAGGTTGCCA GCTGTTGGTG TATCCGGGAG CTTTTAACCT GACAACAGGA 
CCAGCGCACT GGGAACTACT ACAAAGGGGA CGAGCTCTTG ATAATCAAGT CTATGTAGCC 
ACTGCCTCTC CTGCTAGAGA TGAAAATGCT TCCTACATTG CTTGGGGACA CAGCACTGTA 
GTAAATCCAT GGGGGGAAGT TATTGCCAAA GCTGGTGCCG AGGAGGCCAT TCTGTACGCA 
GATATAGATC TGAAGAAGCT TGCGGAAATC CGTGAGCAAA TCCCGGTTTT GAACCAGAAG 
CGCAACGATC TGTATGGCCT GGAGGTGAAA AAGTGA