IGFBP3 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	IGFBP3
Entrez Gene ID	448812
Full Name	insulin like growth factor binding protein 3
Synonyms	IGFBP-3
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 18 Map Location: 18\|18q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005156.1	NP_001005156.1	insulin-like growth factor-binding protein 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells.
Yoo EG, Lee WJ, Kim JH, Chae HW, Hyun SE, Kim DH, Kim HS, Oh Y
Endocrinology152(8)3135-42(2011 Aug)

Expression of the insulin-like growth factor system in skeletal muscle during embryonic and postnatal development in the first filial generation pigs from Erhualian and Yorkshire reciprocal crosses.
Wang L, Zhang G, Lin F, Jiang B, Dong F, Liu H
General and comparative endocrinology173(1)56-62(2011 Aug)

Low-density lipoprotein-related receptor protein 1 (LRP-1) is not required for insulin-like growth factor binding protein 3 (IGFBP-3) to suppress L6 myogenic cell proliferation.
Pampusch MS, Kamanga-Sollo E, Hathaway MR, White ME, Dayton WR
Domestic animal endocrinology40(4)197-204(2011 May)

Genetic variation and association of insulin-like growth factor binding protein-3 with performance in Swine.
Wang W, Meng Q, Hu X, Li N
Biochemical genetics47(3-4)315-21(2009 Apr)

[Developmental expression changes of IGF2 and IGFBP3 genes in adipose tissue of two pig breeds].
Guo YJ, Tang GQ, Li XW, Zhu L, Li MZ
Yi chuan = Hereditas30(5)602-6(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased oxidative stress from high glucose enhances IGFBP-3 expression, inducing apoptosis
Title: Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells.

IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues.
Title: Expression of the insulin-like growth factor system in skeletal muscle during embryonic and postnatal development in the first filial generation pigs from Erhualian and Yorkshire reciprocal crosses.

Even though LRP-1 mRNA and protein levels were dramatically reduced in LRP-1-silenced L6 cells compared with mock-silenced controls, rpIGFPB-3 suppressed proliferation rate to the same extent in both LRP-1-silenced and mock-silenced cultures.
Title: Low-density lipoprotein-related receptor protein 1 (LRP-1) is not required for insulin-like growth factor binding protein 3 (IGFBP-3) to suppress L6 myogenic cell proliferation.

allelic frequency of TAT in Chinese breeds was over 50%; allelic frequency of GGC was over 50% in all European breeds. TAT/TAT pigs scored higher in meat color than GGC/GGC pigs. results implied that IGFBP-3 may affect meat quality & carcass traits.
Title: Genetic variation and association of insulin-like growth factor binding protein-3 with performance in Swine.

The results suggest that the expression of IGF2 and IGFBP3 mRNA in the adipose tissue of pigs exhibits specific developmental changes and different patterns between the two pig breeds.
Title: [Developmental expression changes of IGF2 and IGFBP3 genes in adipose tissue of two pig breeds].

The following IGFBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGFBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00619	NM_001005156.1 Latest version!	Sus scrofa insulin like growth factor binding protein 3 (IGFBP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IGFBP3 ( NM_001005156.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001005156.1 Sus scrofa insulin like growth factor binding protein 3 (IGFBP3), mRNA.

Price & Availability↑

CloneID	OSe00619
Clone ID Related Accession (Same CDS sequence)	NM_001005156.1
Accession Version	NM_001005156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Sus scrofa(pig)
Product	insulin-like growth factor-binding protein 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF085482.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF085482.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGCGGG CGCGCCCCGC GCTCTGGGCC GCGGCGCTGA TCGCGCTGGC GCTGCTCCGC 
GGGCCGTCGG CGGCACGGGC CGGCTCGGGC GCGGTGGGCA CCGGCCCGGT GGTGCGCTGC 
GAGCCGTGCG ACGCGCGCGC CCTGGCCCAG TGCGCGCCGC CGCCCGCCGC CCCCCCGTGC 
GCCGAGCTGG TTCGCGAGCC GGGCTGCGGC TGCTGCCTGA CCTGCGCGCT GCGGGAGGGT 
CAGGCGTGCG GCGTCTACAC CGAGCGATGC GGCGCAGGCC TCCGCTGCCA GCCGCCGCCG 
GGCGAGCCGC GCCCGCTGCA GGCGCTGCTG GACGGCCGCG GGATCTGCGC CAACGCCAGC 
GCCGCCGGCC GCCTGCGCGC CTACCTGCTG CCCGCGCCGC CCGCGCCAGG AAACGGCAGT 
GAGTCCGAGG AGGACCGCAG TGTAGACAGC ATGGAGAACC AGGCCCTGCC CAGCACCCAC 
CGGGTGCCTG ACTCCAAACT CCACTCTATC CACACCAAGA TGGACGTGAT CAAGAAAGGG 
CACGCCAAGG ACAGCCAGCG CTACAAGGTC GACTACGAGT CTCAGAGCAC GGACACCCAG 
AACTTCTCCT CTGAGTCCAA GCGCGAGACG GAATACGGGC CTTGCCGCCG GGAAATGGAG 
GACACGCTGA ACCACCTCAA GTTCCTGAAC ATGCTCAGCC CCCGGGGCAT CCACATCCCC 
AACTGCGACA AGAAGGGCTT CTACAAGAAA AAGCAGTGCC GCCCTTCCAA AGGCAGGAAG 
CGGGGCTTCT GCTGGTGCGT GGATAAGTAC GGGCAGCCCC TCCCGGGCTT CGACGTGAAG 
GGGAAAGGGG ACGTGCACTG CTACAGCATG GAGAGCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005156.1
CDS	107..988
Translation	MQRARPALWAAALIALALLRGPSAARAGSGAVGTGPVVRCEPCDARALAQCAPPPAAPPCAELVREPGCGCCLTCALREGQACGVYTERCGAGLRCQPPPGEPRPLQALLDGRGICANASAAGRLRAYLLPAPPAPGNGSESEEDRSVDSMENQALPSTHRVPDSKLHSIHTKMDVIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNMLSPRGIHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGFDVKGKGDVHCYSMESK

Target ORF information:

RefSeq Version	NM_001005156.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa insulin like growth factor binding protein 3 (IGFBP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005156.1

ORF Insert Sequence:

ATGCAGCGGG CGCGCCCCGC GCTCTGGGCC GCGGCGCTGA TCGCGCTGGC GCTGCTCCGC 
GGGCCGTCGG CGGCACGGGC CGGCTCGGGC GCGGTGGGCA CCGGCCCGGT GGTGCGCTGC 
GAGCCGTGCG ACGCGCGCGC CCTGGCCCAG TGCGCGCCGC CGCCCGCCGC CCCCCCGTGC 
GCCGAGCTGG TTCGCGAGCC GGGCTGCGGC TGCTGCCTGA CCTGCGCGCT GCGGGAGGGT 
CAGGCGTGCG GCGTCTACAC CGAGCGATGC GGCGCAGGCC TCCGCTGCCA GCCGCCGCCG 
GGCGAGCCGC GCCCGCTGCA GGCGCTGCTG GACGGCCGCG GGATCTGCGC CAACGCCAGC 
GCCGCCGGCC GCCTGCGCGC CTACCTGCTG CCCGCGCCGC CCGCGCCAGG AAACGGCAGT 
GAGTCCGAGG AGGACCGCAG TGTAGACAGC ATGGAGAACC AGGCCCTGCC CAGCACCCAC 
CGGGTGCCTG ACTCCAAACT CCACTCTATC CACACCAAGA TGGACGTGAT CAAGAAAGGG 
CACGCCAAGG ACAGCCAGCG CTACAAGGTC GACTACGAGT CTCAGAGCAC GGACACCCAG 
AACTTCTCCT CTGAGTCCAA GCGCGAGACG GAATACGGGC CTTGCCGCCG GGAAATGGAG 
GACACGCTGA ACCACCTCAA GTTCCTGAAC ATGCTCAGCC CCCGGGGCAT CCACATCCCC 
AACTGCGACA AGAAGGGCTT CTACAAGAAA AAGCAGTGCC GCCCTTCCAA AGGCAGGAAG 
CGGGGCTTCT GCTGGTGCGT GGATAAGTAC GGGCAGCCCC TCCCGGGCTT CGACGTGAAG 
GGGAAAGGGG ACGTGCACTG CTACAGCATG GAGAGCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells.
Endocrinology152(8)3135-42(2011 Aug)
Yoo EG,Lee WJ,Kim JH,Chae HW,Hyun SE,Kim DH,Kim HS,Oh Y

Expression of the insulin-like growth factor system in skeletal muscle during embryonic and postnatal development in the first filial generation pigs from Erhualian and Yorkshire reciprocal crosses.
General and comparative endocrinology173(1)56-62(2011 Aug)
Wang L,Zhang G,Lin F,Jiang B,Dong F,Liu H

Low-density lipoprotein-related receptor protein 1 (LRP-1) is not required for insulin-like growth factor binding protein 3 (IGFBP-3) to suppress L6 myogenic cell proliferation.
Domestic animal endocrinology40(4)197-204(2011 May)
Pampusch MS,Kamanga-Sollo E,Hathaway MR,White ME,Dayton WR

Genetic variation and association of insulin-like growth factor binding protein-3 with performance in Swine.
Biochemical genetics47(3-4)315-21(2009 Apr)
Wang W,Meng Q,Hu X,Li N

[Developmental expression changes of IGF2 and IGFBP3 genes in adipose tissue of two pig breeds].
Yi chuan = Hereditas30(5)602-6(2008 May)
Guo YJ,Tang GQ,Li XW,Zhu L,Li MZ