EDN1 cDNA ORF clone, Sus scrofa(Pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDN1
Entrez Gene ID	396915
Full Name	endothelin 1
Synonyms	EDN,ET-1,ET1,PPET1
Gene Type	protein-coding
Organism	Sus scrofa(Pig)
Genome	Chromosome: 7 Map Location: 7\|7p1.3-p1.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_013987639.1	XP_013843093.1	endothelin-1 isoform X2
NM_213882.1	NP_999047.1	endothelin-1 preproprotein
XM_013987638.1	XP_013843092.1	endothelin-1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells.
Dushpanova A, Agostini S, Ciofini E, Cabiati M, Casieri V, Matteucci M, Del Ry S, Clerico A, Berti S, Lionetti V
Scientific reports630048(2016 Jul)

Endothelin-1 impairs coronary arteriolar dilation: Role of p38 kinase-mediated superoxide production from NADPH oxidase.
Thengchaisri N, Hein TW, Ren Y, Kuo L
Journal of molecular and cellular cardiology8675-84(2015 Sep)

Comparison of endothelial nitric oxide synthase and endothelin-1 levels in kidneys removed from living pigs after cardiac arrest and brain death.
Oczkowicz G, Caban A, Budzi?ski G, Suszka-?witek A, Doli?ska B, Czech E, Wiaderkiewicz R, Ryszka F, Cierpka L
Transplantation proceedings46(8)2542-4(2014 Oct)

Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema?
Persson BP, Halldorsdottir H, Lindbom L, Rossi P, Herwald H, Weitzberg E, Oldner A
Acta anaesthesiologica Scandinavica58(5)549-59(2014 May)

Therapeutic hypothermia activates the endothelin and nitric oxide systems after cardiac arrest in a pig model of cardiopulmonary resuscitation.
Zoerner F, Wiklund L, Miclescu A, Martijn C
PloS one8(5)e64792(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

sub-vasomotor concentration of ET-1 leads to vascular dysfunction by impairing endothelium-dependent NO-mediated dilation via p38 kinase-mediated production of superoxide from NADPH oxidase following ETA receptor activation
Title: Endothelin-1 impairs coronary arteriolar dilation: Role of p38 kinase-mediated superoxide production from NADPH oxidase.

ET1 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest.
Title: Comparison of endothelial nitric oxide synthase and endothelin-1 levels in kidneys removed from living pigs after cardiac arrest and brain death.

ET-1 contributes to formation of oedema during experimental sepsis by a novel mechanism involving increased HBP release from neutrophils.
Title: Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema?

Our experiment with isolated porcine ophthalmic ciliary arteries revealed a refractoriness phase to ET after an acute stimulation with ET. This refractoriness was transient and disappeared after 4 h.
Title: Refractoriness to the effect of endothelin-1 in porcine ciliary arteries.

Endothelin-1 expression is upregulated following therapeutic hypothermia after cardiac arrest.
Title: Therapeutic hypothermia activates the endothelin and nitric oxide systems after cardiac arrest in a pig model of cardiopulmonary resuscitation.

The following EDN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe30395	NM_213882.1 Latest version!	Sus scrofa endothelin 1 (EDN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OSe37657	XM_013987638.1 Latest version!	Sus scrofa endothelin 1 (EDN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price
OSe37658	XM_013987639.1 Latest version!	Sus scrofa endothelin 1 (EDN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDN1 ( NM_213882.1 ) cDNA ORF clone, Sus scrofa(Pig) -> NP_999047.1 Sus scrofa endothelin 1 (EDN1), mRNA.

Price & Availability↑

CloneID	OSe30395
Clone ID Related Accession (Same CDS sequence)	NM_213882.1
Accession Version	NM_213882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Sus scrofa(pig)
Product	endothelin-1 preproprotein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X07383.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07383.1, AK348522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2040998, SAMEA3179995 [ECO:0000348] ##Evidence-Data-END##

ATGGATTATT TCCCCATGAT TATCGCTCTG CTGTTTGTGG CTTTCCAAGG AGCTCCAGAA 
ACAGCGGTCC TGGGCGCCGA GCTCAGCCCG GAAGCCGAGA GCCAAGGGGA GACGCCCTCT 
CCCCATGCAT CCTGGAGGCC CCGTCGGTCC AAGCGCTGCT CCTGCTCTTC CCTGATGGAT 
AAAGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACTCC AGAACACATT 
GTCCCATACG GACTTGGAAG CCCTTCTAGG TCCAGGCGAT CCTTAAAGGA TTTGTTTCCT 
GCAAAGGCAG CAGACCGCAG GGATAGATGC CAGTGTGCCA GCCAAAAAGA CAAGAAGTGC 
TGGAGTTTCT GCCAAGCAGG AAAAGAAATC GGCAGGGACC AAGACACAAT GGAGAAACGC 
TGGGATAACC AAAAGAAAGG AACAGACTGT TCCAAGCTTG GAGAGAAGTG TATTCATCGG 
CAGCTGGTGA TGGGAAGAAA AATAAGAAGG TTGGAGGCCA TCAGCAACAG CATCAAAACA 
TCTTTTCACA TCGCCAAGCT GAAAGCCGAG CTCTACAGAG ATAAGAAAGT GACCCATAAC 
CGAACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999047.1
CDS	75..686
Translation	MDYFPMIIALLFVAFQGAPETAVLGAELSPEAESQGETPSPHASWRPRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHIVPYGLGSPSRSRRSLKDLFPAKAADRRDRCQCASQKDKKCWSFCQAGKEIGRDQDTMEKRWDNQKKGTDCSKLGEKCIHRQLVMGRKIRRLEAISNSIKTSFHIAKLKAELYRDKKVTHNRTH

Target ORF information:

RefSeq Version	NM_213882.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa endothelin 1 (EDN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213882.1

ORF Insert Sequence:

ATGGATTATT TCCCCATGAT TATCGCTCTG CTGTTTGTGG CTTTCCAAGG AGCTCCAGAA 
ACAGCGGTCC TGGGCGCCGA GCTCAGCCCG GAAGCCGAGA GCCAAGGGGA GACGCCCTCT 
CCCCATGCAT CCTGGAGGCC CCGTCGGTCC AAGCGCTGCT CCTGCTCTTC CCTGATGGAT 
AAAGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACTCC AGAACACATT 
GTCCCATACG GACTTGGAAG CCCTTCTAGG TCCAGGCGAT CCTTAAAGGA TTTGTTTCCT 
GCAAAGGCAG CAGACCGCAG GGATAGATGC CAGTGTGCCA GCCAAAAAGA CAAGAAGTGC 
TGGAGTTTCT GCCAAGCAGG AAAAGAAATC GGCAGGGACC AAGACACAAT GGAGAAACGC 
TGGGATAACC AAAAGAAAGG AACAGACTGT TCCAAGCTTG GAGAGAAGTG TATTCATCGG 
CAGCTGGTGA TGGGAAGAAA AATAAGAAGG TTGGAGGCCA TCAGCAACAG CATCAAAACA 
TCTTTTCACA TCGCCAAGCT GAAAGCCGAG CTCTACAGAG ATAAGAAAGT GACCCATAAC 
CGAACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN1 ( XM_013987638.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013843092.1 Sus scrofa endothelin 1 (EDN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe37657
Clone ID Related Accession (Same CDS sequence)	XM_013987638.1
Accession Version	XM_013987638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	endothelin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001886400.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCACA CAGGGAAGGA GAGAGGAAAA GACACAGGCA TCCAAGGTGA CTTAATGGAT 
TATTTCCCCA TGATTATCGC TCTGCTGTTT GTGGCTTTCC AAGGAGCTCC AGAAACAGCG 
GTCCTGGGCG CCGAGCTCAG CCCGGGAGCC GAGAGCCAAG GGGAGACGCC CTCTCCCCAT 
GCATCCTGGA GGCCCCGTCG GTCCAAGCGC TGCTCCTGCT CTTCCCTGAT GGATAAAGAG 
TGTGTCTACT TCTGCCACCT GGACATCATC TGGGTCAACA CTCCAGAACA CATTGTCCCA 
TACGGACTTG GAAGCCCTTC TAGGTCCAGG CGATCCTTAA AGGATTTGTT TCCTGCAAAG 
GCAGCAGACC GCAGGGATAG ATGCCAGTGT GCCAGCCAAA AAGACAAGAA GTGCTGGAGT 
TTCTGCCAAG CAGGAAAAGA AATCGGCAGG GACCAAGACA CAATGGAGAA ACGCTGGGAT 
AACCAAAAGA AAGGAACAGA CTGTTCCAAG CTTGGAGAGA AGTGTATTCA TCGGCAGCTG 
GTGATGGGAA GAAAAATAAG AAGGTTGGAG GCCATCAGCA ACAGCATCAA AACATCTTTT 
CACATCGCCA AGCTGAAAGC CGAGCTCTAC AGAGATAAGA AAGTGACCCA TAACCGAACA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013843092.1
CDS	202..867
Translation	MDHTGKERGKDTGIQGDLMDYFPMIIALLFVAFQGAPETAVLGAELSPGAESQGETPSPHASWRPRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHIVPYGLGSPSRSRRSLKDLFPAKAADRRDRCQCASQKDKKCWSFCQAGKEIGRDQDTMEKRWDNQKKGTDCSKLGEKCIHRQLVMGRKIRRLEAISNSIKTSFHIAKLKAELYRDKKVTHNRTH

Target ORF information:

RefSeq Version	XM_013987638.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa endothelin 1 (EDN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013987638.1

ORF Insert Sequence:

ATGGATCACA CAGGGAAGGA GAGAGGAAAA GACACAGGCA TCCAAGGTGA CTTAATGGAT 
TATTTCCCCA TGATTATCGC TCTGCTGTTT GTGGCTTTCC AAGGAGCTCC AGAAACAGCG 
GTCCTGGGCG CCGAGCTCAG CCCGGGAGCC GAGAGCCAAG GGGAGACGCC CTCTCCCCAT 
GCATCCTGGA GGCCCCGTCG GTCCAAGCGC TGCTCCTGCT CTTCCCTGAT GGATAAAGAG 
TGTGTCTACT TCTGCCACCT GGACATCATC TGGGTCAACA CTCCAGAACA CATTGTCCCA 
TACGGACTTG GAAGCCCTTC TAGGTCCAGG CGATCCTTAA AGGATTTGTT TCCTGCAAAG 
GCAGCAGACC GCAGGGATAG ATGCCAGTGT GCCAGCCAAA AAGACAAGAA GTGCTGGAGT 
TTCTGCCAAG CAGGAAAAGA AATCGGCAGG GACCAAGACA CAATGGAGAA ACGCTGGGAT 
AACCAAAAGA AAGGAACAGA CTGTTCCAAG CTTGGAGAGA AGTGTATTCA TCGGCAGCTG 
GTGATGGGAA GAAAAATAAG AAGGTTGGAG GCCATCAGCA ACAGCATCAA AACATCTTTT 
CACATCGCCA AGCTGAAAGC CGAGCTCTAC AGAGATAAGA AAGTGACCCA TAACCGAACA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN1 ( XM_013987639.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013843093.1 Sus scrofa endothelin 1 (EDN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe37658
Clone ID Related Accession (Same CDS sequence)	XM_013987639.1
Accession Version	XM_013987639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	endothelin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001886400.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTATT TCCCCATGAT TATCGCTCTG CTGTTTGTGG CTTTCCAAGG AGCTCCAGAA 
ACAGCGGTCC TGGGCGCCGA GCTCAGCCCG GGAGCCGAGA GCCAAGGGGA GACGCCCTCT 
CCCCATGCAT CCTGGAGGCC CCGTCGGTCC AAGCGCTGCT CCTGCTCTTC CCTGATGGAT 
AAAGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACTCC AGAACACATT 
GTCCCATACG GACTTGGAAG CCCTTCTAGG TCCAGGCGAT CCTTAAAGGA TTTGTTTCCT 
GCAAAGGCAG CAGACCGCAG GGATAGATGC CAGTGTGCCA GCCAAAAAGA CAAGAAGTGC 
TGGAGTTTCT GCCAAGCAGG AAAAGAAATC GGCAGGGACC AAGACACAAT GGAGAAACGC 
TGGGATAACC AAAAGAAAGG AACAGACTGT TCCAAGCTTG GAGAGAAGTG TATTCATCGG 
CAGCTGGTGA TGGGAAGAAA AATAAGAAGG TTGGAGGCCA TCAGCAACAG CATCAAAACA 
TCTTTTCACA TCGCCAAGCT GAAAGCCGAG CTCTACAGAG ATAAGAAAGT GACCCATAAC 
CGAACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013843093.1
CDS	51..662
Translation	MDYFPMIIALLFVAFQGAPETAVLGAELSPGAESQGETPSPHASWRPRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHIVPYGLGSPSRSRRSLKDLFPAKAADRRDRCQCASQKDKKCWSFCQAGKEIGRDQDTMEKRWDNQKKGTDCSKLGEKCIHRQLVMGRKIRRLEAISNSIKTSFHIAKLKAELYRDKKVTHNRTH

Target ORF information:

RefSeq Version	XM_013987639.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa endothelin 1 (EDN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013987639.1

ORF Insert Sequence:

ATGGATTATT TCCCCATGAT TATCGCTCTG CTGTTTGTGG CTTTCCAAGG AGCTCCAGAA 
ACAGCGGTCC TGGGCGCCGA GCTCAGCCCG GGAGCCGAGA GCCAAGGGGA GACGCCCTCT 
CCCCATGCAT CCTGGAGGCC CCGTCGGTCC AAGCGCTGCT CCTGCTCTTC CCTGATGGAT 
AAAGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACTCC AGAACACATT 
GTCCCATACG GACTTGGAAG CCCTTCTAGG TCCAGGCGAT CCTTAAAGGA TTTGTTTCCT 
GCAAAGGCAG CAGACCGCAG GGATAGATGC CAGTGTGCCA GCCAAAAAGA CAAGAAGTGC 
TGGAGTTTCT GCCAAGCAGG AAAAGAAATC GGCAGGGACC AAGACACAAT GGAGAAACGC 
TGGGATAACC AAAAGAAAGG AACAGACTGT TCCAAGCTTG GAGAGAAGTG TATTCATCGG 
CAGCTGGTGA TGGGAAGAAA AATAAGAAGG TTGGAGGCCA TCAGCAACAG CATCAAAACA 
TCTTTTCACA TCGCCAAGCT GAAAGCCGAG CTCTACAGAG ATAAGAAAGT GACCCATAAC 
CGAACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells.
Scientific reports630048(2016 Jul)
Dushpanova A,Agostini S,Ciofini E,Cabiati M,Casieri V,Matteucci M,Del Ry S,Clerico A,Berti S,Lionetti V

Endothelin-1 impairs coronary arteriolar dilation: Role of p38 kinase-mediated superoxide production from NADPH oxidase.
Journal of molecular and cellular cardiology8675-84(2015 Sep)
Thengchaisri N,Hein TW,Ren Y,Kuo L

Comparison of endothelial nitric oxide synthase and endothelin-1 levels in kidneys removed from living pigs after cardiac arrest and brain death.
Transplantation proceedings46(8)2542-4(2014 Oct)
Oczkowicz G,Caban A,Budzi?ski G,Suszka-?witek A,Doli?ska B,Czech E,Wiaderkiewicz R,Ryszka F,Cierpka L

Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema?
Acta anaesthesiologica Scandinavica58(5)549-59(2014 May)
Persson BP,Halldorsdottir H,Lindbom L,Rossi P,Herwald H,Weitzberg E,Oldner A

Therapeutic hypothermia activates the endothelin and nitric oxide systems after cardiac arrest in a pig model of cardiopulmonary resuscitation.
PloS one8(5)e64792(2013)
Zoerner F,Wiklund L,Miclescu A,Martijn C