RASGRF1 cDNA ORF clone, Sus scrofa(Pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF1
Entrez Gene ID	100157221
Full Name	Ras protein specific guanine nucleotide releasing factor 1
Gene Type	protein-coding
Organism	Sus scrofa(Pig)
Genome	Chromosome: 7 Map Location: 7\|7

mRNA and Protein(s)

mRNA	Protein	Name
XM_003128446.6	XP_003128494.3	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_013988986.1	XP_013844440.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_013988989.1	XP_013844443.1	ras-specific guanine nucleotide-releasing factor 1 isoform X5
XM_013988987.1	XP_013844441.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3
XM_013988986.2	XP_013844440.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_021098844.1	XP_020954503.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3
XM_003128446.5	XP_003128494.3	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_013988988.1	XP_013844442.1	ras-specific guanine nucleotide-releasing factor 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe13869	XM_003128446.5 Latest version!	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe222891	XM_013988986.2 Latest version!	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe222892	XM_003128446.6 Latest version!	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe222893	XM_021098844.1 Latest version!	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe38100	XM_013988986.1 Latest version!	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe38101	XM_013988987.1 Latest version!	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe38102	XM_013988988.1 Latest version!	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe38103	XM_013988989.1 Latest version!	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF1 ( XM_003128446.5 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_003128494.3 Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe13869
Clone ID Related Accession (Same CDS sequence)	XM_003128446.5
Accession Version	XM_003128446.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003128446.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGTGA CCCACCGTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAAGAGGG GACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA TATTATCAGA GAGATTCGCC AGTTTCAACA AACAGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAGTCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003128494.3
CDS	1..3774
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_003128446.5
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003128446.5

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGTGA CCCACCGTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAAGAGGG GACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA TATTATCAGA GAGATTCGCC AGTTTCAACA AACAGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAGTCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_013988986.2 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844440.1 Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe222891
Clone ID Related Accession (Same CDS sequence)	XM_013988986.2
Accession Version	XM_013988986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013988986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTGTA AGTGCCTCTC CCAGATGCCT 
CTGCACCCTG CTCATGGGGC GGAGGTGGCC TTCGGGCGTC CTGGGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013844440.1
CDS	404..4234
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQLFHPSVLSGRLSSAWAPQSPCGLCLRGAAPMHSPACPSAPRALLHLPPSHGFLVHRRRRLLCLRGPPFCLSPHPDCLPSFGLPVGLCKCLSQMPLHPAHGAEVAFGRPGH

Target ORF information:

RefSeq Version	XM_013988986.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013988986.2

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTGTA AGTGCCTCTC CCAGATGCCT 
CTGCACCCTG CTCATGGGGC GGAGGTGGCC TTCGGGCGTC CTGGGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_003128446.6 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_003128494.3 Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

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CloneID	OSe222892
Clone ID Related Accession (Same CDS sequence)	XM_003128446.6
Accession Version	XM_003128446.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003128446.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGTGA CCCACCGTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAAGAGGG GACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA TATTATCAGA GAGATTCGCC AGTTTCAACA AACAGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAGTCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003128494.3
CDS	462..4235
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_003128446.6
Organism	Sus scrofa(pig)
Definition	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003128446.6

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGTGA CCCACCGTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAAGAGGG GACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA TATTATCAGA GAGATTCGCC AGTTTCAACA AACAGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAGTCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_021098844.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_020954503.1 Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe222893
Clone ID Related Accession (Same CDS sequence)	XM_021098844.1
Accession Version	XM_021098844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATAAAT GGAGGCTCAG TTTCCTCTGC 
AATAATCAGC AGGAGAGCCT TGCTTTGAAC CCACGCAATC TGCCTCCGGA GGGCTGCCCA 
CATCACTGCC TGGCATTCGG CCATCTCTGT TCAAATCTAC CTCTTTTTGA CCAGGTACAC 
AGACTGTGTG CCTCTGTGAT GATGATAATC ATGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954503.1
CDS	1..3399
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDKWRLSFLCNNQQESLALNPRNLPPEGCPHHCLAFGHLCSNLPLFDQVHRLCASVMMIIMA

Target ORF information:

RefSeq Version	XM_021098844.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098844.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGC 
GGCCAAAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATAAAT GGAGGCTCAG TTTCCTCTGC 
AATAATCAGC AGGAGAGCCT TGCTTTGAAC CCACGCAATC TGCCTCCGGA GGGCTGCCCA 
CATCACTGCC TGGCATTCGG CCATCTCTGT TCAAATCTAC CTCTTTTTGA CCAGGTACAC 
AGACTGTGTG CCTCTGTGAT GATGATAATC ATGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_013988986.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844440.1 Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe38100
Clone ID Related Accession (Same CDS sequence)	XM_013988986.1
Accession Version	XM_013988986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTGTA AGTGCCTCTC CCAGATGCCT 
CTGCACCCTG CTCATGGGGC GGAGGTGGCC TTCGGGCGTC CTGGGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013844440.1
CDS	453..4283
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQLFHPSVLSGRLSSAWAPQSPCGLCLRGAAPMHSPACPSAPRALLHLPPSHGFLVHRRRRLLCLRGPPFCLSPHPDCLPSFGLPVGLCKCLSQMPLHPAHGAEVAFGRPGH

Target ORF information:

RefSeq Version	XM_013988986.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013988986.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTGTA AGTGCCTCTC CCAGATGCCT 
CTGCACCCTG CTCATGGGGC GGAGGTGGCC TTCGGGCGTC CTGGGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_013988987.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844441.1 Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

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CloneID	OSe38101
Clone ID Related Accession (Same CDS sequence)	XM_013988987.1
Accession Version	XM_013988987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013844441.1
CDS	1..3759
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQLFHPSVLSGRLSSAWAPQSPCGLCLRGAAPMHSPACPSAPRALLHLPPSHGFLVHRRRRLLCLRGPPFCLSPHPDCLPSFGLPVGL

Target ORF information:

RefSeq Version	XM_013988987.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013988987.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGTT GTTCCACCCA TCTGTGCTGT CCGGCCGTCT GTCCTCCGCC 
TGGGCTCCCC AGAGCCCCTG TGGACTGTGC TTGCGGGGTG CTGCCCCCAT GCATTCACCA 
GCCTGTCCGT CCGCCCCCAG GGCGCTGCTC CATCTGCCTC CTTCACATGG CTTCCTCGTC 
CACCGCCGCC GCCGCCTCCT CTGCCTCCGT GGTCCCCCAT TCTGTCTGAG TCCCCATCCT 
GACTGTCTTC CCTCTTTCGG GTTACCCGTG GGCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_013988988.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844442.1 Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe38102
Clone ID Related Accession (Same CDS sequence)	XM_013988988.1
Accession Version	XM_013988988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAACTTGAA CAGGAAGCCC GCGGGAGGAA CGCAGGAACC AGGCACTGCC 
GTAAAAGCCC TTCCCCTCTG CTCCCCTAAG TTGAGGATGG AAGCGGGTCT GCCAACTGAC 
CAGCAGGCCG TCCCCGTGCA CCCTCCAAAG GACGGGGACC TCCATCTATC TGCATGGATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013844442.1
CDS	1..3663
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNLNRKPAGGTQEPGTAVKALPLCSPKLRMEAGLPTDQQAVPVHPPKDGDLHLSAWI

Target ORF information:

RefSeq Version	XM_013988988.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013988988.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCGCTCTTTT CCTACAATAA TGGAGTCGTC ATGACCTCCT GTCGCGAGTT GGACAATAAC 
CGCAGTGCCC TGTCAGCAGC GTCCGCCTTT GCCATAGCGA CTGCGGGCGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA TCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCACCCGAC 
CAGAGGAACG GAGACAAGGA GTTTGTGATC CGCAGAGCGG CCACCAATCG CGTCCTGAAC 
GTGCTCCGCC ACTGGGTCTC CAAGCATTCT CAGGACTTTG AGACCAACGA CGAGCTCAAA 
TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTACT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACGCTGG AGGAGATCAC ACAGCTGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCTGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTGG ACCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT TGGACAAGGC TGGATGAAAC TGGAAAAGAA CGAAAGGACC 
CCTTACATCA TGAAAACCAC CAAGCATTTC AACGATATCA GCAACCTGAT TGCTTCCGAA 
ATCATCCGCA ATGAGGACAT CAACGCGAGG GTGAGCGCCA TTGAGAAGTG GGTGGCTGTG 
GCGGACATAT GCCGCTGCTT ACACAACTAC AATGCTGTTC TGGAGATCAC CTCGTCCATG 
AACCGGAGTG CAATCTTCCG ACTGAAAAAG ACGTGGCTCA AAGTATCTAA GCAGACAAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAACTTGAA CAGGAAGCCC GCGGGAGGAA CGCAGGAACC AGGCACTGCC 
GTAAAAGCCC TTCCCCTCTG CTCCCCTAAG TTGAGGATGG AAGCGGGTCT GCCAACTGAC 
CAGCAGGCCG TCCCCGTGCA CCCTCCAAAG GACGGGGACC TCCATCTATC TGCATGGATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_013988989.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844443.1 Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe38103
Clone ID Related Accession (Same CDS sequence)	XM_013988989.1
Accession Version	XM_013988989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535196.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGGGTTT 
CCACCCGACC AGAGGAACGG AGACAAGGAG TTTGTGATCC GCAGAGCGGC CACCAATCGC 
GTCCTGAACG TGCTCCGCCA CTGGGTCTCC AAGCATTCTC AGGACTTTGA GACCAACGAC 
GAGCTCAAAT GCAAGGTGAT CAGCTTCCTG GAGGAGGTCA TGCACGACCC AGAGCTACTG 
ACCCAGGAGC GGAAGGCTGC GGCCAACATC ATCAGGACTC TGACCCAGGA TGACCCAGGC 
GACAACCAGA TCACGCTGGA GGAGATCACA CAGCTGGCTG AAGGCGTGAA GGCCGAGCCC 
TTTGAAAACC ACTCTGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTGGA CCACCTCGTC 
TTCAAGAAGA TCCCTTATGA GGAGTTCTTT GGACAAGGCT GGATGAAACT GGAAAAGAAC 
GAAAGGACCC CTTACATCAT GAAAACCACC AAGCATTTCA ACGATATCAG CAACCTGATT 
GCTTCCGAAA TCATCCGCAA TGAGGACATC AACGCGAGGG TGAGCGCCAT TGAGAAGTGG 
GTGGCTGTGG CGGACATATG CCGCTGCTTA CACAACTACA ATGCTGTTCT GGAGATCACC 
TCGTCCATGA ACCGGAGTGC AATCTTCCGA CTGAAAAAGA CGTGGCTCAA AGTATCTAAG 
CAGACAAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCAGAGGG CAGATTTAAG 
AATCTGAGAG AAGCTCTGAA AAAACAGTTG TTCCACCCAT CTGTGCTGTC CGGCCGTCTG 
TCCTCCGCCT GGGCTCCCCA GAGCCCCTGT GGACTGTGCT TGCGGGGTGC TGCCCCCATG 
CATTCACCAG CCTGTCCGTC CGCCCCCAGG GCGCTGCTCC ATCTGCCTCC TTCACATGGC 
TTCCTCGTCC ACCGCCGCCG CCGCCTCCTC TGCCTCCGTG GTCCCCCATT CTGTCTGAGT 
CCCCATCCTG ACTGTCTTCC CTCTTTCGGG TTACCCGTGG GCCTGTGTAA GTGCCTCTCC 
CAGATGCCTC TGCACCCTGC TCATGGGGCG GAGGTGGCCT TCGGGCGTCC TGGGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013844443.1
CDS	1..3660
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQPVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEDPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFASKIPDEGDTTAEKPEEPSALRKQSSEISMREESDNDQNQSDDGDAETSPTKSPTTPKSVKSKNSSGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQLAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQLFHPSVLSGRLSSAWAPQSPCGLCLRGAAPMHSPACPSAPRALLHLPPSHGFLVHRRRRLLCLRGPPFCLSPHPDCLPSFGLPVGLCKCLSQMPLHPAHGAEVAFGRPGH

Target ORF information:

RefSeq Version	XM_013988989.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013988989.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGACT GCTGGCACGC 
AAGGACGGCA CGCGCAAAGG CTACCTAAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCATCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTACGCTCT CAGCCAAGGA GCCACTGGAG AAACAGCATT ACTTCACCGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGATTG CGACGAATGG 
GTGGCGGCCA TTGCTCACGC CAGCTACCGG ACCCTGGCCA CAGAGCATGA GGCACTGATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTA 
CGACAGCAGA TCGAGGATGG GGAGATCGAG ATTGAGCGGC TGAAGGCAGA GATCGCGTCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGCCCG TCACCCCCAA TGATGAAGAC 
AGTGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCGCCGC ACGCCGACAG CATGCGGAAA 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTCA ACAACTTCCT GCGCCCGCTG CGCATGGCTG CCAGCTCCAA GAAGCCGCCC 
ATCACGCACG ACGATGTCAG CAGCATCTTC CTGAACAGTG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACTCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTTGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT TGCCCACTGC AAACAGAACC GCGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC CCACACCCCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAACTGTCC 
AGGATAATGC ATGATGAAGT AAGCGAAACA GAGAACATCC GAAAAAACCT GGCCATTGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT CGTCAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCCGAA AAGGGCAAGA TCACCCGGGG TCGCCTGGGT 
TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTTTCTAA GCATCTGATC 
ATCTGTACCA GAGGCTCTGG CGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CCTTGTTGGA GGACCCAGAA AGCACGGAGG AAGAAGCCAA AGGATCCGGC 
CAAGACATTG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAGGACTC CCCGCCCTTC 
ACAGTCATCC TCGTGGCCTC ATCCAGGCAG GAGAAGGCGG CCTGGACCAG TGACATCAGC 
CAGTGCGTGG ATAACATCCG GTGCAACGGG CTCATGATGA ACGCGTTTGA GGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TGTATTGTGA TGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTCCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTGC TGGAGAGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACG GCCGTTGTGG TCCTGGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTCGCCAGC 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCCCCCAAGT CCCCACGAGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCGT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ATATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCTTTCAG CAAGGCCACC 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA GCAAGATTCC AGATGAGGGC 
GATACGACCG CTGAGAAGCC TGAAGAACCC TCAGCTCTCA GAAAGCAGAG CTCAGAAATC 
TCCATGAGGG AAGAGTCAGA TAACGACCAA AACCAGAGTG ACGATGGTGA CGCCGAGACT 
TCACCAACCA AGTCTCCGAC CACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGGGTTT 
CCACCCGACC AGAGGAACGG AGACAAGGAG TTTGTGATCC GCAGAGCGGC CACCAATCGC 
GTCCTGAACG TGCTCCGCCA CTGGGTCTCC AAGCATTCTC AGGACTTTGA GACCAACGAC 
GAGCTCAAAT GCAAGGTGAT CAGCTTCCTG GAGGAGGTCA TGCACGACCC AGAGCTACTG 
ACCCAGGAGC GGAAGGCTGC GGCCAACATC ATCAGGACTC TGACCCAGGA TGACCCAGGC 
GACAACCAGA TCACGCTGGA GGAGATCACA CAGCTGGCTG AAGGCGTGAA GGCCGAGCCC 
TTTGAAAACC ACTCTGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTGGA CCACCTCGTC 
TTCAAGAAGA TCCCTTATGA GGAGTTCTTT GGACAAGGCT GGATGAAACT GGAAAAGAAC 
GAAAGGACCC CTTACATCAT GAAAACCACC AAGCATTTCA ACGATATCAG CAACCTGATT 
GCTTCCGAAA TCATCCGCAA TGAGGACATC AACGCGAGGG TGAGCGCCAT TGAGAAGTGG 
GTGGCTGTGG CGGACATATG CCGCTGCTTA CACAACTACA ATGCTGTTCT GGAGATCACC 
TCGTCCATGA ACCGGAGTGC AATCTTCCGA CTGAAAAAGA CGTGGCTCAA AGTATCTAAG 
CAGACAAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCAGAGGG CAGATTTAAG 
AATCTGAGAG AAGCTCTGAA AAAACAGTTG TTCCACCCAT CTGTGCTGTC CGGCCGTCTG 
TCCTCCGCCT GGGCTCCCCA GAGCCCCTGT GGACTGTGCT TGCGGGGTGC TGCCCCCATG 
CATTCACCAG CCTGTCCGTC CGCCCCCAGG GCGCTGCTCC ATCTGCCTCC TTCACATGGC 
TTCCTCGTCC ACCGCCGCCG CCGCCTCCTC TGCCTCCGTG GTCCCCCATT CTGTCTGAGT 
CCCCATCCTG ACTGTCTTCC CTCTTTCGGG TTACCCGTGG GCCTGTGTAA GTGCCTCTCC 
CAGATGCCTC TGCACCCTGC TCATGGGGCG GAGGTGGCCT TCGGGCGTCC TGGGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

RASGRF1 cDNA ORF clone, Sus scrofa(Pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム