ankrd27 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankrd27
Entrez Gene ID	103372977
Full Name	ankyrin repeat domain 27
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008302765.1	XP_008300987.1	ankyrin repeat domain-containing protein 27 isoform X1
XM_008302766.1	XP_008300988.1	ankyrin repeat domain-containing protein 27 isoform X2

Pathways from BioSystems
Gene Ontology

The following ankrd27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankrd27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt60715	XM_008302765.1 Latest version!	Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OSt60716	XM_008302766.1 Latest version!	Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankrd27 ( XM_008302765.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008300987.1 Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSt60715
Clone ID Related Accession (Same CDS sequence)	XM_008302765.1
Accession Version	XM_008302765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007579606.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TGTCAGAGGA GGGTGATGAG AATCCAGATC TCCAGCTCCT TATGGCTGTA 
TATGATGAGA ACATCCTGAA GAATCCTTTC TACTTGGCGC TAGAGAAGCA GAGGCCGGAC 
CTCTGTAGCC GAGTGGCTGA GCTCCACGGC ATTGTTCTGG TGCCTTGTTG TGGAAGTTTG 
ACTGTTGGCA GCTACTCAGA CTCTCACTTT GACAGCTATG TCCTACAGGC TGTGGAGGAT 
GGATACAGAA GTGTGGATGG CAAGGAGGTC AGGATCCAGG ACAGGCAGGT TCTGCTGGGG 
TTCGGCTCCC CGAGTCCCGT GTCTGTCCCC ATTCTGTTTG AGGAAACCTT CTACAATGAC 
CAGGAGCAGA GCTACAGTAT CCTGTGCATC TCCAGGCCCA TGGAGGTGGA TTCGAGCCCA 
GCCGAAGTCA GCTCCCCTCC CCCCTACTGC CTGAAAAGCC TGGAGGATGT CAGGGAGTTC 
CTGGGCCGCC ATGCCCAGAA ACTGGACAAG TTCATCCACA GCTTCTGTCA GGCATTCAAA 
GAGCACGAGA GGAAGGGACT CCGACACCAC ATAGATTCAG TCAACAGTCT TTACACTAAA 
TGTCTTCAGT GTCTGCTCAG AGACTCTCGC TTGAAACTTC TGGCTAAGCA GGAGCTTCAG 
ATGACTCTTC TCAAACAGGC GGTGGAGATA TATGTTCATC ATGGTATCCA TGATTTAATC 
TTTAACTTTG TGGGAACGCT TGAAGCTAGC CAGGATGCTG CCTTCAACAA AACCACCAGG 
AGTCTGCAGG AGCTGCAGCA AAAGGACCTG GGAGTCAAAC CTGAGTTCTG TATAAACTTG 
TCTAGGGCAA AGAGGGACCT GAGTCAGCTG AACCAGCAGA CATCGCCCCT CCTCAAGCTG 
CTTTGCCTGC GCAGAGTCGC CCTGACCGCC ACGCAGACCC CCAGACGCAC TGTCAGTATA 
GAAGCTGTGA GTGCAGATGA CCTCCTCTCT GTCGTCCTTT ATCTGCTGGT GAAGACGGAA 
ATTCCCAACT GGATGGCCAA CCTGAGCTAC ATCAGGAACT TCTGCTTCAG CCAGTCCAGT 
AAAGATGAGC TCAGCTACTG TCTGAGCACC TTCGAGGCTG CAGTGGAGTA CATCAACCTG 
GGAAAGCTTC AGGACACGCA CTCTGGCTCA GGCGAACTCA ACGACAAGGC ACTGTTCAAA 
GAGAGGATGA GTCTACTGTC TCAGAGCGCT GCCATGCCCA TCCACTGTCT GTTTGAGCAC 
ATAGCAAATG GAAACGAAGC TGAGGTGGAA CGTCTGCTGA GTGAAGGAGA GGGTGATGAG 
GACGTCAGGA TGTGCCACCC CCTCTGCTCC TGTGACTTCT GTGACCTCCA GCTGTCCGGA 
CGGTTGAATG ATCCGTCCAT CATCACGCCG TTCTCCAGGG ATGACCGAGG TTACACGCCT 
TTGCATGCCG CTGCTGTCTG TGGTCAGGCC CAGCTGATTG ACGTGCTGGT TCGTAAAGGA 
GCTCCAGTCA ATGCCACAGA CTACCATGCC CTGACACCAC TGCACCTGGC CTGCCAGCGT 
GGATACCAGG GAGTCTCATT GCTGCTGCTG CACTACAAGG CCAACACAGA CGCTCAGGAC 
AACAACAGCA ACACTCCTCT GCACCTCGCC TGCATGTATG GACATGAGGA TTGTGTGAAG 
GCCTTGGTGT ACTATGATGT CCAAACGTGT CGTCTGGACC TTCAGAACGA TAAAGGTGAC 
ACAGCGCTGC ACATGGCGGC CCGCTGGGGT TACGAGGGAA TCATCCAGGT GCTGCTGGAA 
AACGGAGCCA GTGCCCACAT CCTTAACAGG AGCAAAGAGT CACCACTGCA GTGTGCACTC 
AACTCCAAGA TTGTCATGTT GTTGCAGTCG ACTCAGAACG GACGTCAGCG CAGCGGCAGT 
GGAGTAGACT CTCCAAGTTG CTCTCCTCTA GCCTCAGATT GCAGCAGCCG TCGCTCCTCC 
ATCTCCAGCA CATCCTCTCT GGGCTCAGAG GCCAAACCAG AGGGAGATCG AGTCCGACAC 
AGAGAGGTGG AGAAGCTTCT GCGTGCTGTG GCAGATGATG ATGTGGAGAT GGTGCGCTAC 
CTGCTGGAGT GGATGGATGA AGATGAGGAG GATGAAGGAG ACATTCGTTC TGAAGCTCTG 
CTCTGCCACC CATTGTGTCA GTGTCCAGAC TGCGCTCCCA CTCAGAAGCT GTCCGTGCTG 
CAGGCTGGAG TTCTTGGTGT CAACAGCTGC AACGTTGACG GTTTCACACC ACTTCATGTC 
GCAGCTCTGC ACGGCCACTC GGCGCTGGCT GCTCTGCTAA TCCGCCACGG GGCCAGTGTC 
AATGCCCGCA CCAACCAGAG CGCCACGCCG CTTCACCTGG CCAGCCAGAA TAGCCACATC 
CAGGTGGTGA GGTTTCTGCT GGAGTGCAAT GCCAAACTGA ATAAGAAGGA TCAGTATGGC 
AACACACCTC TGATCCATGC CTGTCTCCGT GGAAACCTGG AAACAGCCAC CACCCTGTTA 
CAGAGTAATG CGTTGGTGAA CATTGCGAAC CTTCAGGGGA ACACAGCTCT CCATGAGGCG 
GTGCGAGGTG GACACCAGGG CCTCGTGGAG CTGCTGCTGA GGGGCGGAGC CGTATCGGGC 
CTCAGGAATA AAAGGCAGAG AACACCACTG GACTGCGCCT ATGAACTGGG TGGCAAGAAC 
CATGAAATCC TAAGAGCTCT GCAGAAAGCT TCTGGACTTT CCCCTGATGC TGAACCAATC 
AAACTCCTCT CTGTGCCCAA AGGAGCCCTG GCTCATTCAT TTGTGCAGCG CCTGAGGCTC 
CAGGATCACT CCAACGGCAG GAGACAGAAG CTGGCCCAGT CCAGGATCCA GCAGATGAAG 
AAAGGTTCCT CTGGTCCTTC CTCCAGGTGT TCACCAGCCC TGAACCAGGT GAATCCAGAC 
AGGAGGAGGC TGAGGCGGGG GGAGACGGTG GAGGTCAGCA GCCCGTCAGG AAGCTTTGAT 
GCTGGGCTCC GTCAGAAGGA GCGGCCCCTG GTTCGCTGTC ACACGCTGGA CTCTGAAGTG 
CACACTCCAG AGCCACCTGA CTCTCCACAT GCTCCAAACA CGACTGAATA CATTTTAGAA 
GATCATGATC AGGCAAATTG CATACACACT CCCAAAACTA GCGAAGGTGC TGTGCCAGCA 
GGTGTCCAGA CATCCAGAGA GGCAGCTGAT GCTGAAGCTG ACACCAGTGA ACTGTGTTCC 
ACTGACGTCT CTAACTGCAG CACAGGTAGT GAGAGCTCGC CCCTCCCGCC CTCTGATGCA 
TCGCCACAAG CCAGCAGCTC ACCTGACTTC TCTTCTACAC ACATGCTGAC CGCTCAGTCT 
GCTCCGCCTC ACACCCACCG GCCTGTGAAC AGAGAATCAG AGGAAGCCGC CGTCAGCCAG 
ACTCAGCCTC TTGCTGCAGC TGAAGCCTTG CAGTCAGAAA GCGAGGATGA AGACCCCAAC 
GCCACTGACA TGCACCCCGT CATCCAGACT GACAGTGGCG ATCAGAGAAA CGGCAACCAA 
ACAGCAGAGA CGAGCGCACC GTCAAATCAA GGAGGCTATG ACCAGAATGA AGGTGTTGTA 
GAGCAACTAT TAGAAGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008300987.1
CDS	230..3853
Translation	MKLMSEEGDENPDLQLLMAVYDENILKNPFYLALEKQRPDLCSRVAELHGIVLVPCCGSLTVGSYSDSHFDSYVLQAVEDGYRSVDGKEVRIQDRQVLLGFGSPSPVSVPILFEETFYNDQEQSYSILCISRPMEVDSSPAEVSSPPPYCLKSLEDVREFLGRHAQKLDKFIHSFCQAFKEHERKGLRHHIDSVNSLYTKCLQCLLRDSRLKLLAKQELQMTLLKQAVEIYVHHGIHDLIFNFVGTLEASQDAAFNKTTRSLQELQQKDLGVKPEFCINLSRAKRDLSQLNQQTSPLLKLLCLRRVALTATQTPRRTVSIEAVSADDLLSVVLYLLVKTEIPNWMANLSYIRNFCFSQSSKDELSYCLSTFEAAVEYINLGKLQDTHSGSGELNDKALFKERMSLLSQSAAMPIHCLFEHIANGNEAEVERLLSEGEGDEDVRMCHPLCSCDFCDLQLSGRLNDPSIITPFSRDDRGYTPLHAAAVCGQAQLIDVLVRKGAPVNATDYHALTPLHLACQRGYQGVSLLLLHYKANTDAQDNNSNTPLHLACMYGHEDCVKALVYYDVQTCRLDLQNDKGDTALHMAARWGYEGIIQVLLENGASAHILNRSKESPLQCALNSKIVMLLQSTQNGRQRSGSGVDSPSCSPLASDCSSRRSSISSTSSLGSEAKPEGDRVRHREVEKLLRAVADDDVEMVRYLLEWMDEDEEDEGDIRSEALLCHPLCQCPDCAPTQKLSVLQAGVLGVNSCNVDGFTPLHVAALHGHSALAALLIRHGASVNARTNQSATPLHLASQNSHIQVVRFLLECNAKLNKKDQYGNTPLIHACLRGNLETATTLLQSNALVNIANLQGNTALHEAVRGGHQGLVELLLRGGAVSGLRNKRQRTPLDCAYELGGKNHEILRALQKASGLSPDAEPIKLLSVPKGALAHSFVQRLRLQDHSNGRRQKLAQSRIQQMKKGSSGPSSRCSPALNQVNPDRRRLRRGETVEVSSPSGSFDAGLRQKERPLVRCHTLDSEVHTPEPPDSPHAPNTTEYILEDHDQANCIHTPKTSEGAVPAGVQTSREAADAEADTSELCSTDVSNCSTGSESSPLPPSDASPQASSSPDFSSTHMLTAQSAPPHTHRPVNRESEEAAVSQTQPLAAAEALQSESEDEDPNATDMHPVIQTDSGDQRNGNQTAETSAPSNQGGYDQNEGVVEQLLEEN

Target ORF information:

RefSeq Version	XM_008302765.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008302765.1

ORF Insert Sequence:

ATGAAGCTGA TGTCAGAGGA GGGTGATGAG AATCCAGATC TCCAGCTCCT TATGGCTGTA 
TATGATGAGA ACATCCTGAA GAATCCTTTC TACTTGGCGC TAGAGAAGCA GAGGCCGGAC 
CTCTGTAGCC GAGTGGCTGA GCTCCACGGC ATTGTTCTGG TGCCTTGTTG TGGAAGTTTG 
ACTGTTGGCA GCTACTCAGA CTCTCACTTT GACAGCTATG TCCTACAGGC TGTGGAGGAT 
GGATACAGAA GTGTGGATGG CAAGGAGGTC AGGATCCAGG ACAGGCAGGT TCTGCTGGGG 
TTCGGCTCCC CGAGTCCCGT GTCTGTCCCC ATTCTGTTTG AGGAAACCTT CTACAATGAC 
CAGGAGCAGA GCTACAGTAT CCTGTGCATC TCCAGGCCCA TGGAGGTGGA TTCGAGCCCA 
GCCGAAGTCA GCTCCCCTCC CCCCTACTGC CTGAAAAGCC TGGAGGATGT CAGGGAGTTC 
CTGGGCCGCC ATGCCCAGAA ACTGGACAAG TTCATCCACA GCTTCTGTCA GGCATTCAAA 
GAGCACGAGA GGAAGGGACT CCGACACCAC ATAGATTCAG TCAACAGTCT TTACACTAAA 
TGTCTTCAGT GTCTGCTCAG AGACTCTCGC TTGAAACTTC TGGCTAAGCA GGAGCTTCAG 
ATGACTCTTC TCAAACAGGC GGTGGAGATA TATGTTCATC ATGGTATCCA TGATTTAATC 
TTTAACTTTG TGGGAACGCT TGAAGCTAGC CAGGATGCTG CCTTCAACAA AACCACCAGG 
AGTCTGCAGG AGCTGCAGCA AAAGGACCTG GGAGTCAAAC CTGAGTTCTG TATAAACTTG 
TCTAGGGCAA AGAGGGACCT GAGTCAGCTG AACCAGCAGA CATCGCCCCT CCTCAAGCTG 
CTTTGCCTGC GCAGAGTCGC CCTGACCGCC ACGCAGACCC CCAGACGCAC TGTCAGTATA 
GAAGCTGTGA GTGCAGATGA CCTCCTCTCT GTCGTCCTTT ATCTGCTGGT GAAGACGGAA 
ATTCCCAACT GGATGGCCAA CCTGAGCTAC ATCAGGAACT TCTGCTTCAG CCAGTCCAGT 
AAAGATGAGC TCAGCTACTG TCTGAGCACC TTCGAGGCTG CAGTGGAGTA CATCAACCTG 
GGAAAGCTTC AGGACACGCA CTCTGGCTCA GGCGAACTCA ACGACAAGGC ACTGTTCAAA 
GAGAGGATGA GTCTACTGTC TCAGAGCGCT GCCATGCCCA TCCACTGTCT GTTTGAGCAC 
ATAGCAAATG GAAACGAAGC TGAGGTGGAA CGTCTGCTGA GTGAAGGAGA GGGTGATGAG 
GACGTCAGGA TGTGCCACCC CCTCTGCTCC TGTGACTTCT GTGACCTCCA GCTGTCCGGA 
CGGTTGAATG ATCCGTCCAT CATCACGCCG TTCTCCAGGG ATGACCGAGG TTACACGCCT 
TTGCATGCCG CTGCTGTCTG TGGTCAGGCC CAGCTGATTG ACGTGCTGGT TCGTAAAGGA 
GCTCCAGTCA ATGCCACAGA CTACCATGCC CTGACACCAC TGCACCTGGC CTGCCAGCGT 
GGATACCAGG GAGTCTCATT GCTGCTGCTG CACTACAAGG CCAACACAGA CGCTCAGGAC 
AACAACAGCA ACACTCCTCT GCACCTCGCC TGCATGTATG GACATGAGGA TTGTGTGAAG 
GCCTTGGTGT ACTATGATGT CCAAACGTGT CGTCTGGACC TTCAGAACGA TAAAGGTGAC 
ACAGCGCTGC ACATGGCGGC CCGCTGGGGT TACGAGGGAA TCATCCAGGT GCTGCTGGAA 
AACGGAGCCA GTGCCCACAT CCTTAACAGG AGCAAAGAGT CACCACTGCA GTGTGCACTC 
AACTCCAAGA TTGTCATGTT GTTGCAGTCG ACTCAGAACG GACGTCAGCG CAGCGGCAGT 
GGAGTAGACT CTCCAAGTTG CTCTCCTCTA GCCTCAGATT GCAGCAGCCG TCGCTCCTCC 
ATCTCCAGCA CATCCTCTCT GGGCTCAGAG GCCAAACCAG AGGGAGATCG AGTCCGACAC 
AGAGAGGTGG AGAAGCTTCT GCGTGCTGTG GCAGATGATG ATGTGGAGAT GGTGCGCTAC 
CTGCTGGAGT GGATGGATGA AGATGAGGAG GATGAAGGAG ACATTCGTTC TGAAGCTCTG 
CTCTGCCACC CATTGTGTCA GTGTCCAGAC TGCGCTCCCA CTCAGAAGCT GTCCGTGCTG 
CAGGCTGGAG TTCTTGGTGT CAACAGCTGC AACGTTGACG GTTTCACACC ACTTCATGTC 
GCAGCTCTGC ACGGCCACTC GGCGCTGGCT GCTCTGCTAA TCCGCCACGG GGCCAGTGTC 
AATGCCCGCA CCAACCAGAG CGCCACGCCG CTTCACCTGG CCAGCCAGAA TAGCCACATC 
CAGGTGGTGA GGTTTCTGCT GGAGTGCAAT GCCAAACTGA ATAAGAAGGA TCAGTATGGC 
AACACACCTC TGATCCATGC CTGTCTCCGT GGAAACCTGG AAACAGCCAC CACCCTGTTA 
CAGAGTAATG CGTTGGTGAA CATTGCGAAC CTTCAGGGGA ACACAGCTCT CCATGAGGCG 
GTGCGAGGTG GACACCAGGG CCTCGTGGAG CTGCTGCTGA GGGGCGGAGC CGTATCGGGC 
CTCAGGAATA AAAGGCAGAG AACACCACTG GACTGCGCCT ATGAACTGGG TGGCAAGAAC 
CATGAAATCC TAAGAGCTCT GCAGAAAGCT TCTGGACTTT CCCCTGATGC TGAACCAATC 
AAACTCCTCT CTGTGCCCAA AGGAGCCCTG GCTCATTCAT TTGTGCAGCG CCTGAGGCTC 
CAGGATCACT CCAACGGCAG GAGACAGAAG CTGGCCCAGT CCAGGATCCA GCAGATGAAG 
AAAGGTTCCT CTGGTCCTTC CTCCAGGTGT TCACCAGCCC TGAACCAGGT GAATCCAGAC 
AGGAGGAGGC TGAGGCGGGG GGAGACGGTG GAGGTCAGCA GCCCGTCAGG AAGCTTTGAT 
GCTGGGCTCC GTCAGAAGGA GCGGCCCCTG GTTCGCTGTC ACACGCTGGA CTCTGAAGTG 
CACACTCCAG AGCCACCTGA CTCTCCACAT GCTCCAAACA CGACTGAATA CATTTTAGAA 
GATCATGATC AGGCAAATTG CATACACACT CCCAAAACTA GCGAAGGTGC TGTGCCAGCA 
GGTGTCCAGA CATCCAGAGA GGCAGCTGAT GCTGAAGCTG ACACCAGTGA ACTGTGTTCC 
ACTGACGTCT CTAACTGCAG CACAGGTAGT GAGAGCTCGC CCCTCCCGCC CTCTGATGCA 
TCGCCACAAG CCAGCAGCTC ACCTGACTTC TCTTCTACAC ACATGCTGAC CGCTCAGTCT 
GCTCCGCCTC ACACCCACCG GCCTGTGAAC AGAGAATCAG AGGAAGCCGC CGTCAGCCAG 
ACTCAGCCTC TTGCTGCAGC TGAAGCCTTG CAGTCAGAAA GCGAGGATGA AGACCCCAAC 
GCCACTGACA TGCACCCCGT CATCCAGACT GACAGTGGCG ATCAGAGAAA CGGCAACCAA 
ACAGCAGAGA CGAGCGCACC GTCAAATCAA GGAGGCTATG ACCAGAATGA AGGTGTTGTA 
GAGCAACTAT TAGAAGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd27 ( XM_008302766.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008300988.1 Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSt60716
Clone ID Related Accession (Same CDS sequence)	XM_008302766.1
Accession Version	XM_008302766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007579606.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAT ATGATGAGAA CATCCTGAAG AATCCTTTCT ACTTGGCGCT AGAGAAGCAG 
AGGCCGGACC TCTGTAGCCG AGTGGCTGAG CTCCACGGCA TTGTTCTGGT GCCTTGTTGT 
GGAAGTTTGA CTGTTGGCAG CTACTCAGAC TCTCACTTTG ACAGCTATGT CCTACAGGCT 
GTGGAGGATG GATACAGAAG TGTGGATGGC AAGGAGGTCA GGATCCAGGA CAGGCAGGTT 
CTGCTGGGGT TCGGCTCCCC GAGTCCCGTG TCTGTCCCCA TTCTGTTTGA GGAAACCTTC 
TACAATGACC AGGAGCAGAG CTACAGTATC CTGTGCATCT CCAGGCCCAT GGAGGTGGAT 
TCGAGCCCAG CCGAAGTCAG CTCCCCTCCC CCCTACTGCC TGAAAAGCCT GGAGGATGTC 
AGGGAGTTCC TGGGCCGCCA TGCCCAGAAA CTGGACAAGT TCATCCACAG CTTCTGTCAG 
GCATTCAAAG AGCACGAGAG GAAGGGACTC CGACACCACA TAGATTCAGT CAACAGTCTT 
TACACTAAAT GTCTTCAGTG TCTGCTCAGA GACTCTCGCT TGAAACTTCT GGCTAAGCAG 
GAGCTTCAGA TGACTCTTCT CAAACAGGCG GTGGAGATAT ATGTTCATCA TGGTATCCAT 
GATTTAATCT TTAACTTTGT GGGAACGCTT GAAGCTAGCC AGGATGCTGC CTTCAACAAA 
ACCACCAGGA GTCTGCAGGA GCTGCAGCAA AAGGACCTGG GAGTCAAACC TGAGTTCTGT 
ATAAACTTGT CTAGGGCAAA GAGGGACCTG AGTCAGCTGA ACCAGCAGAC ATCGCCCCTC 
CTCAAGCTGC TTTGCCTGCG CAGAGTCGCC CTGACCGCCA CGCAGACCCC CAGACGCACT 
GTCAGTATAG AAGCTGTGAG TGCAGATGAC CTCCTCTCTG TCGTCCTTTA TCTGCTGGTG 
AAGACGGAAA TTCCCAACTG GATGGCCAAC CTGAGCTACA TCAGGAACTT CTGCTTCAGC 
CAGTCCAGTA AAGATGAGCT CAGCTACTGT CTGAGCACCT TCGAGGCTGC AGTGGAGTAC 
ATCAACCTGG GAAAGCTTCA GGACACGCAC TCTGGCTCAG GCGAACTCAA CGACAAGGCA 
CTGTTCAAAG AGAGGATGAG TCTACTGTCT CAGAGCGCTG CCATGCCCAT CCACTGTCTG 
TTTGAGCACA TAGCAAATGG AAACGAAGCT GAGGTGGAAC GTCTGCTGAG TGAAGGAGAG 
GGTGATGAGG ACGTCAGGAT GTGCCACCCC CTCTGCTCCT GTGACTTCTG TGACCTCCAG 
CTGTCCGGAC GGTTGAATGA TCCGTCCATC ATCACGCCGT TCTCCAGGGA TGACCGAGGT 
TACACGCCTT TGCATGCCGC TGCTGTCTGT GGTCAGGCCC AGCTGATTGA CGTGCTGGTT 
CGTAAAGGAG CTCCAGTCAA TGCCACAGAC TACCATGCCC TGACACCACT GCACCTGGCC 
TGCCAGCGTG GATACCAGGG AGTCTCATTG CTGCTGCTGC ACTACAAGGC CAACACAGAC 
GCTCAGGACA ACAACAGCAA CACTCCTCTG CACCTCGCCT GCATGTATGG ACATGAGGAT 
TGTGTGAAGG CCTTGGTGTA CTATGATGTC CAAACGTGTC GTCTGGACCT TCAGAACGAT 
AAAGGTGACA CAGCGCTGCA CATGGCGGCC CGCTGGGGTT ACGAGGGAAT CATCCAGGTG 
CTGCTGGAAA ACGGAGCCAG TGCCCACATC CTTAACAGGA GCAAAGAGTC ACCACTGCAG 
TGTGCACTCA ACTCCAAGAT TGTCATGTTG TTGCAGTCGA CTCAGAACGG ACGTCAGCGC 
AGCGGCAGTG GAGTAGACTC TCCAAGTTGC TCTCCTCTAG CCTCAGATTG CAGCAGCCGT 
CGCTCCTCCA TCTCCAGCAC ATCCTCTCTG GGCTCAGAGG CCAAACCAGA GGGAGATCGA 
GTCCGACACA GAGAGGTGGA GAAGCTTCTG CGTGCTGTGG CAGATGATGA TGTGGAGATG 
GTGCGCTACC TGCTGGAGTG GATGGATGAA GATGAGGAGG ATGAAGGAGA CATTCGTTCT 
GAAGCTCTGC TCTGCCACCC ATTGTGTCAG TGTCCAGACT GCGCTCCCAC TCAGAAGCTG 
TCCGTGCTGC AGGCTGGAGT TCTTGGTGTC AACAGCTGCA ACGTTGACGG TTTCACACCA 
CTTCATGTCG CAGCTCTGCA CGGCCACTCG GCGCTGGCTG CTCTGCTAAT CCGCCACGGG 
GCCAGTGTCA ATGCCCGCAC CAACCAGAGC GCCACGCCGC TTCACCTGGC CAGCCAGAAT 
AGCCACATCC AGGTGGTGAG GTTTCTGCTG GAGTGCAATG CCAAACTGAA TAAGAAGGAT 
CAGTATGGCA ACACACCTCT GATCCATGCC TGTCTCCGTG GAAACCTGGA AACAGCCACC 
ACCCTGTTAC AGAGTAATGC GTTGGTGAAC ATTGCGAACC TTCAGGGGAA CACAGCTCTC 
CATGAGGCGG TGCGAGGTGG ACACCAGGGC CTCGTGGAGC TGCTGCTGAG GGGCGGAGCC 
GTATCGGGCC TCAGGAATAA AAGGCAGAGA ACACCACTGG ACTGCGCCTA TGAACTGGGT 
GGCAAGAACC ATGAAATCCT AAGAGCTCTG CAGAAAGCTT CTGGACTTTC CCCTGATGCT 
GAACCAATCA AACTCCTCTC TGTGCCCAAA GGAGCCCTGG CTCATTCATT TGTGCAGCGC 
CTGAGGCTCC AGGATCACTC CAACGGCAGG AGACAGAAGC TGGCCCAGTC CAGGATCCAG 
CAGATGAAGA AAGGTTCCTC TGGTCCTTCC TCCAGGTGTT CACCAGCCCT GAACCAGGTG 
AATCCAGACA GGAGGAGGCT GAGGCGGGGG GAGACGGTGG AGGTCAGCAG CCCGTCAGGA 
AGCTTTGATG CTGGGCTCCG TCAGAAGGAG CGGCCCCTGG TTCGCTGTCA CACGCTGGAC 
TCTGAAGTGC ACACTCCAGA GCCACCTGAC TCTCCACATG CTCCAAACAC GACTGAATAC 
ATTTTAGAAG ATCATGATCA GGCAAATTGC ATACACACTC CCAAAACTAG CGAAGGTGCT 
GTGCCAGCAG GTGTCCAGAC ATCCAGAGAG GCAGCTGATG CTGAAGCTGA CACCAGTGAA 
CTGTGTTCCA CTGACGTCTC TAACTGCAGC ACAGGTAGTG AGAGCTCGCC CCTCCCGCCC 
TCTGATGCAT CGCCACAAGC CAGCAGCTCA CCTGACTTCT CTTCTACACA CATGCTGACC 
GCTCAGTCTG CTCCGCCTCA CACCCACCGG CCTGTGAACA GAGAATCAGA GGAAGCCGCC 
GTCAGCCAGA CTCAGCCTCT TGCTGCAGCT GAAGCCTTGC AGTCAGAAAG CGAGGATGAA 
GACCCCAACG CCACTGACAT GCACCCCGTC ATCCAGACTG ACAGTGGCGA TCAGAGAAAC 
GGCAACCAAA CAGCAGAGAC GAGCGCACCG TCAAATCAAG GAGGCTATGA CCAGAATGAA 
GGTGTTGTAG AGCAACTATT AGAAGAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008300988.1
CDS	1483..5055
Translation	MAVYDENILKNPFYLALEKQRPDLCSRVAELHGIVLVPCCGSLTVGSYSDSHFDSYVLQAVEDGYRSVDGKEVRIQDRQVLLGFGSPSPVSVPILFEETFYNDQEQSYSILCISRPMEVDSSPAEVSSPPPYCLKSLEDVREFLGRHAQKLDKFIHSFCQAFKEHERKGLRHHIDSVNSLYTKCLQCLLRDSRLKLLAKQELQMTLLKQAVEIYVHHGIHDLIFNFVGTLEASQDAAFNKTTRSLQELQQKDLGVKPEFCINLSRAKRDLSQLNQQTSPLLKLLCLRRVALTATQTPRRTVSIEAVSADDLLSVVLYLLVKTEIPNWMANLSYIRNFCFSQSSKDELSYCLSTFEAAVEYINLGKLQDTHSGSGELNDKALFKERMSLLSQSAAMPIHCLFEHIANGNEAEVERLLSEGEGDEDVRMCHPLCSCDFCDLQLSGRLNDPSIITPFSRDDRGYTPLHAAAVCGQAQLIDVLVRKGAPVNATDYHALTPLHLACQRGYQGVSLLLLHYKANTDAQDNNSNTPLHLACMYGHEDCVKALVYYDVQTCRLDLQNDKGDTALHMAARWGYEGIIQVLLENGASAHILNRSKESPLQCALNSKIVMLLQSTQNGRQRSGSGVDSPSCSPLASDCSSRRSSISSTSSLGSEAKPEGDRVRHREVEKLLRAVADDDVEMVRYLLEWMDEDEEDEGDIRSEALLCHPLCQCPDCAPTQKLSVLQAGVLGVNSCNVDGFTPLHVAALHGHSALAALLIRHGASVNARTNQSATPLHLASQNSHIQVVRFLLECNAKLNKKDQYGNTPLIHACLRGNLETATTLLQSNALVNIANLQGNTALHEAVRGGHQGLVELLLRGGAVSGLRNKRQRTPLDCAYELGGKNHEILRALQKASGLSPDAEPIKLLSVPKGALAHSFVQRLRLQDHSNGRRQKLAQSRIQQMKKGSSGPSSRCSPALNQVNPDRRRLRRGETVEVSSPSGSFDAGLRQKERPLVRCHTLDSEVHTPEPPDSPHAPNTTEYILEDHDQANCIHTPKTSEGAVPAGVQTSREAADAEADTSELCSTDVSNCSTGSESSPLPPSDASPQASSSPDFSSTHMLTAQSAPPHTHRPVNRESEEAAVSQTQPLAAAEALQSESEDEDPNATDMHPVIQTDSGDQRNGNQTAETSAPSNQGGYDQNEGVVEQLLEEN

Target ORF information:

RefSeq Version	XM_008302766.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008302766.1

ORF Insert Sequence:

ATGGCTGTAT ATGATGAGAA CATCCTGAAG AATCCTTTCT ACTTGGCGCT AGAGAAGCAG 
AGGCCGGACC TCTGTAGCCG AGTGGCTGAG CTCCACGGCA TTGTTCTGGT GCCTTGTTGT 
GGAAGTTTGA CTGTTGGCAG CTACTCAGAC TCTCACTTTG ACAGCTATGT CCTACAGGCT 
GTGGAGGATG GATACAGAAG TGTGGATGGC AAGGAGGTCA GGATCCAGGA CAGGCAGGTT 
CTGCTGGGGT TCGGCTCCCC GAGTCCCGTG TCTGTCCCCA TTCTGTTTGA GGAAACCTTC 
TACAATGACC AGGAGCAGAG CTACAGTATC CTGTGCATCT CCAGGCCCAT GGAGGTGGAT 
TCGAGCCCAG CCGAAGTCAG CTCCCCTCCC CCCTACTGCC TGAAAAGCCT GGAGGATGTC 
AGGGAGTTCC TGGGCCGCCA TGCCCAGAAA CTGGACAAGT TCATCCACAG CTTCTGTCAG 
GCATTCAAAG AGCACGAGAG GAAGGGACTC CGACACCACA TAGATTCAGT CAACAGTCTT 
TACACTAAAT GTCTTCAGTG TCTGCTCAGA GACTCTCGCT TGAAACTTCT GGCTAAGCAG 
GAGCTTCAGA TGACTCTTCT CAAACAGGCG GTGGAGATAT ATGTTCATCA TGGTATCCAT 
GATTTAATCT TTAACTTTGT GGGAACGCTT GAAGCTAGCC AGGATGCTGC CTTCAACAAA 
ACCACCAGGA GTCTGCAGGA GCTGCAGCAA AAGGACCTGG GAGTCAAACC TGAGTTCTGT 
ATAAACTTGT CTAGGGCAAA GAGGGACCTG AGTCAGCTGA ACCAGCAGAC ATCGCCCCTC 
CTCAAGCTGC TTTGCCTGCG CAGAGTCGCC CTGACCGCCA CGCAGACCCC CAGACGCACT 
GTCAGTATAG AAGCTGTGAG TGCAGATGAC CTCCTCTCTG TCGTCCTTTA TCTGCTGGTG 
AAGACGGAAA TTCCCAACTG GATGGCCAAC CTGAGCTACA TCAGGAACTT CTGCTTCAGC 
CAGTCCAGTA AAGATGAGCT CAGCTACTGT CTGAGCACCT TCGAGGCTGC AGTGGAGTAC 
ATCAACCTGG GAAAGCTTCA GGACACGCAC TCTGGCTCAG GCGAACTCAA CGACAAGGCA 
CTGTTCAAAG AGAGGATGAG TCTACTGTCT CAGAGCGCTG CCATGCCCAT CCACTGTCTG 
TTTGAGCACA TAGCAAATGG AAACGAAGCT GAGGTGGAAC GTCTGCTGAG TGAAGGAGAG 
GGTGATGAGG ACGTCAGGAT GTGCCACCCC CTCTGCTCCT GTGACTTCTG TGACCTCCAG 
CTGTCCGGAC GGTTGAATGA TCCGTCCATC ATCACGCCGT TCTCCAGGGA TGACCGAGGT 
TACACGCCTT TGCATGCCGC TGCTGTCTGT GGTCAGGCCC AGCTGATTGA CGTGCTGGTT 
CGTAAAGGAG CTCCAGTCAA TGCCACAGAC TACCATGCCC TGACACCACT GCACCTGGCC 
TGCCAGCGTG GATACCAGGG AGTCTCATTG CTGCTGCTGC ACTACAAGGC CAACACAGAC 
GCTCAGGACA ACAACAGCAA CACTCCTCTG CACCTCGCCT GCATGTATGG ACATGAGGAT 
TGTGTGAAGG CCTTGGTGTA CTATGATGTC CAAACGTGTC GTCTGGACCT TCAGAACGAT 
AAAGGTGACA CAGCGCTGCA CATGGCGGCC CGCTGGGGTT ACGAGGGAAT CATCCAGGTG 
CTGCTGGAAA ACGGAGCCAG TGCCCACATC CTTAACAGGA GCAAAGAGTC ACCACTGCAG 
TGTGCACTCA ACTCCAAGAT TGTCATGTTG TTGCAGTCGA CTCAGAACGG ACGTCAGCGC 
AGCGGCAGTG GAGTAGACTC TCCAAGTTGC TCTCCTCTAG CCTCAGATTG CAGCAGCCGT 
CGCTCCTCCA TCTCCAGCAC ATCCTCTCTG GGCTCAGAGG CCAAACCAGA GGGAGATCGA 
GTCCGACACA GAGAGGTGGA GAAGCTTCTG CGTGCTGTGG CAGATGATGA TGTGGAGATG 
GTGCGCTACC TGCTGGAGTG GATGGATGAA GATGAGGAGG ATGAAGGAGA CATTCGTTCT 
GAAGCTCTGC TCTGCCACCC ATTGTGTCAG TGTCCAGACT GCGCTCCCAC TCAGAAGCTG 
TCCGTGCTGC AGGCTGGAGT TCTTGGTGTC AACAGCTGCA ACGTTGACGG TTTCACACCA 
CTTCATGTCG CAGCTCTGCA CGGCCACTCG GCGCTGGCTG CTCTGCTAAT CCGCCACGGG 
GCCAGTGTCA ATGCCCGCAC CAACCAGAGC GCCACGCCGC TTCACCTGGC CAGCCAGAAT 
AGCCACATCC AGGTGGTGAG GTTTCTGCTG GAGTGCAATG CCAAACTGAA TAAGAAGGAT 
CAGTATGGCA ACACACCTCT GATCCATGCC TGTCTCCGTG GAAACCTGGA AACAGCCACC 
ACCCTGTTAC AGAGTAATGC GTTGGTGAAC ATTGCGAACC TTCAGGGGAA CACAGCTCTC 
CATGAGGCGG TGCGAGGTGG ACACCAGGGC CTCGTGGAGC TGCTGCTGAG GGGCGGAGCC 
GTATCGGGCC TCAGGAATAA AAGGCAGAGA ACACCACTGG ACTGCGCCTA TGAACTGGGT 
GGCAAGAACC ATGAAATCCT AAGAGCTCTG CAGAAAGCTT CTGGACTTTC CCCTGATGCT 
GAACCAATCA AACTCCTCTC TGTGCCCAAA GGAGCCCTGG CTCATTCATT TGTGCAGCGC 
CTGAGGCTCC AGGATCACTC CAACGGCAGG AGACAGAAGC TGGCCCAGTC CAGGATCCAG 
CAGATGAAGA AAGGTTCCTC TGGTCCTTCC TCCAGGTGTT CACCAGCCCT GAACCAGGTG 
AATCCAGACA GGAGGAGGCT GAGGCGGGGG GAGACGGTGG AGGTCAGCAG CCCGTCAGGA 
AGCTTTGATG CTGGGCTCCG TCAGAAGGAG CGGCCCCTGG TTCGCTGTCA CACGCTGGAC 
TCTGAAGTGC ACACTCCAGA GCCACCTGAC TCTCCACATG CTCCAAACAC GACTGAATAC 
ATTTTAGAAG ATCATGATCA GGCAAATTGC ATACACACTC CCAAAACTAG CGAAGGTGCT 
GTGCCAGCAG GTGTCCAGAC ATCCAGAGAG GCAGCTGATG CTGAAGCTGA CACCAGTGAA 
CTGTGTTCCA CTGACGTCTC TAACTGCAGC ACAGGTAGTG AGAGCTCGCC CCTCCCGCCC 
TCTGATGCAT CGCCACAAGC CAGCAGCTCA CCTGACTTCT CTTCTACACA CATGCTGACC 
GCTCAGTCTG CTCCGCCTCA CACCCACCGG CCTGTGAACA GAGAATCAGA GGAAGCCGCC 
GTCAGCCAGA CTCAGCCTCT TGCTGCAGCT GAAGCCTTGC AGTCAGAAAG CGAGGATGAA 
GACCCCAACG CCACTGACAT GCACCCCGTC ATCCAGACTG ACAGTGGCGA TCAGAGAAAC 
GGCAACCAAA CAGCAGAGAC GAGCGCACCG TCAAATCAAG GAGGCTATGA CCAGAATGAA 
GGTGTTGTAG AGCAACTATT AGAAGAGAAC TGA