spef2 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	spef2
Entrez Gene ID	108938116
Full Name	sperm flagellar protein 2-like
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029259498.1	XP_029115331.1	sperm flagellar protein 2 isoform X2
XM_029259501.1	XP_029115334.1	sperm flagellar protein 2 isoform X5
XM_018758460.2	XP_018613976.2	sperm flagellar protein 2 isoform X1
XM_029259499.1	XP_029115332.1	sperm flagellar protein 2 isoform X3
XM_018758460.1	XP_018613976.1	LOW QUALITY PROTEIN: sperm flagellar protein 2-like
XM_029259500.1	XP_029115333.1	sperm flagellar protein 2 isoform X4

Pathways from BioSystems
Gene Ontology

The following spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc59488	XM_018758460.1 Latest version!	Scleropages formosus sperm flagellar protein 2-like (LOC108938116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSc135155	XM_018758460.2 Latest version!	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc135156	XM_029259498.1 Latest version!	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc135157	XM_029259499.1 Latest version!	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc135158	XM_029259500.1 Latest version!	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc135159	XM_029259501.1 Latest version!	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spef2 ( XM_018758460.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018613976.1 Scleropages formosus sperm flagellar protein 2-like (LOC108938116), mRNA.

Price & Availability↑

CloneID	OSc59488
Clone ID Related Accession (Same CDS sequence)	XM_018758460.1
Accession Version	XM_018758460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	LOW QUALITY PROTEIN: sperm flagellar protein 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372020.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GGTGCCGGGC GGCCCCCTTC GGGGGGGNNN NNNNNNNGAG 
AGCGCTTCCC CAGAGCCGAG CCCACAGGAG CTCGAGAAGC TGGAAGTCCT CAACAACCAG 
GACTATGATG ACTACACGAG TATGACCGGT GAGTGGGCCT GGCCTGAGGA GGGGGATGCG 
AAGCAGCCCC CGCCCAACAA CAACATCCTG GGTCACGTGG TGCACCGGCT CAACGTGATC 
GTGGACCCCC CTAAGCCGGA GACCCCCCCG CCCTCGTTCC CCAGTTTCAC GCTGAAGGCC 
TCCGTCCTGG GAAAGGTGTT TGCAGGGAAA AGCGGCTGTC TGGCAAGGAT GGCGCAAGTC 
CACGGCGTCC ACGTCCTGTC GACCAGCATT CTGATCCAGG AGGCGCTGGA AGCGTTCCTG 
GCCGGAGAGA AGGTGCCGCA GGCGCCCCCG AAGGAGCCGC AGTATCCCGA GGGATCCGTC 
TGTCTCAGGA GGACTGCGAA GAAGAAGGAT GTTGCCGAGA AGGAGAAATC TTCACGCCCC 
GACTCAGGCC ATGTCCTCCA AAGCCCCCAG CAGGAAAATG CCAAGAGGAC AGCTTCCAAG 
TCTGAGGTAC TGACCTCACC TCCCCAGCTG TCGGCGAGGG CTCAGCACGG AGCCGCCCTG 
GACGAGGTCC TCGGCAAGGG GCAGGCCATC CCAGACCAGC TCCTCGTGGA CATTGTTCTG 
GAAGCCATCA GGCGGGTCCC TCCAGAGTCG GGCTGGATCC TCGATGGGTT CCCCATGGAC 
CTGGATCAGG CCAAGCTCCT GGAGAAGGCT CTCAGTGGGA TTGACCCAGA GAAGGTGGAA 
AGGAAGAAGA ATAACAAGAA GATCAACCTT GTCGTGGACC CCCATGCGAA GAAAGAGGCC 
CCACCCCCTC TACCTGCCCT GGACGTCGTT CTGCTTATGG ACTTGTCCGA TGACCTCGTT 
CTGGACCGTG CTGCAAAGAA AGCCAATGAG ATGGAAGGCA AGCTGGTGCA AATTCAGACT 
CTGGACGAGA TGTGCGCCTT GGTGGAGGCG GGATCTCCAC CAGAGCCGTG CCCCTCGACG 
CAAACTCCTG ACCTTCGGGA CAAGAGCCTG GAGATGGCGC AGGTCCAGCA CAGGATCGCA 
GCCTTCCACG ACACCTGGCC CAAGCTGGAG TGGTGGTACT CCAACAGGCA GAAGATCCTG 
GTGAAGGTGA ACGCGGACGA AGAGCCCGAC GCCGTCTTCA AGGAAGTGGA ATCCATCGTG 
TACAAGATCA TGGCGCCGCC GGCAGACAAG GGTGTAGACG CGACGCCTAC AGCCCAGACT 
GCTCAGGCCC CTGGTGTCAC TCCACCTCCT CTTGCAATGT CCTCCTCTCC AGCGGGACCC 
CCGGCGCAAA AATCCCCATC GGAGTCCCGA ATCCACTCCC CTGATGAAGG CGTCCCGGCC 
GATGCCGCCC CAGAAGCGCC CGTCCCCGAA GTCCCTCAAG GCAAGAGACC GAGCCCCAGA 
GCGCTCCCTA TGAGCCAGTC CACCGAGGTG GTCTACGTGG ACAAGCCGCT GCCCAAGGAA 
TTCCCGGAAT ACCTCGTTCC ATACTGGGAG AACACGTGCA CGTCGTATGT GACAAATATC 
AAAGCGGCGA TGCAGAACCT CCGCGCCGAG CGGGACCTGA TCATCCAGCA TCTCTATGAC 
ATCAGGCAGG AGTTCAAGCA CTACTTGATG AGACCGGACC TGAAGCAGGA GTTTGTGACC 
CAGTGGCAGC AGGACTACAA CAGCATCCCT GAGGACATGA GGGCTGACGA AGACACCAAG 
GCAGAGCTGC ACCAGCGCCT CAATGACCTG AGCGAGCGGC TGTGGGACAT CAGTGAGCGG 
CGCAAGGAGG AGGCGCTGCA GGAGCGGACC GTGCTCATGG GCGACGGCTG GCTGGAGGAC 
CACACGGCCA TGCTCATCAA CCACTTCTCC ACCCTCATGC AGGTGGAGGT GGACAGGTTC 
CAGGACACGG TGCATCTGCT GCGGGACTAT TACAGGGGCA TGTCCGGGCA GAACCTCCCG 
GAAACGCGGT TCGCGCACGT CCCCATGGTG GACGTCGCCG AAGACGCTCG GGACGAGGAC 
CCCACTGGCT CTGTGCAGCC TGAGCAGCAG GACACGAGCG CTGGAACAAG CGAAGAAGAG 
GAGAGCAAGA CTAAAATAAT CCCTCTGATC CCACGGAGAC CTCGGTCGGG AGAAGCCGCG 
CTGATAAAGC CGAACGCCTC GACGGACCCG CACCCGGACG AGCAGCTTTT GCATGACATT 
TGGCAGACGG CCGTGACCGC TGTTAATAAC CTGGTGTCGC AGGAGATGCC CCAGCTGGAG 
CCCGAGGAGG GTAAGGAGGC CCAGTACGTG GCGGAGCAGG GGCCGTCTCA GCCCGCGGCC 
CAGAGCGCCA AGGACAACGA GAAGCCTGGA AACACAAAAG GAGGTGTCCC AGAGCCCTCG 
GCCCCAGAGC AAGCGCCTAG CCCACCGCCC CAAGTCGAGG AGGACGTGGA GGTTGTCCGC 
AGAAAGACAG TGAAAGCCCA GATTCGCAAG GAGTTTGCCG CAGCACTCAG TCACGAGGAC 
AACGCGGTGA AAGTACGCCT GGAGCTTGTC CGAGCGCGAG CGCTGCGCGT ATTGAGGAGC 
CTCCACTGCC AAGCTGACCA AGCTTATAGT GCCATGGAGG AGTGGCTTCA CACCCGCTTT 
CAAGCAGAGA TGAACAGCAT CAACCAGTTG GTGGAGGTGT CGCGTCACCA CATCGAAGCG 
GCCGCCCAGA TCCAGCACGA GCTCGTGCTG GAGGGTCCCG ACTTCTTCAT TGATGGGGAT 
ACGCGCGTCG TAACCGCTTC NNNNNNNNNN TACGCCGCCG CCGTGCCCGC CCCCCTGGGA 
GCTCCCGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018613976.1
CDS	101..4372
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKVQEERRMKDIEKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQEELNRDERLLACLTRQSQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLVPGGPLRGnullnullnullnullESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKSSRPDSGHVLQSPQQENAKRTASKSEVLTSPPQLSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALVEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGGVPEPSAPEQAPSPPPQVEEDVEVVRRKTVKAQIRKEFAAALSHEDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVTAnullnullnullnullYAAAVPAPLGAPG

Target ORF information:

RefSeq Version	XM_018758460.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar protein 2-like (LOC108938116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018758460.1

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GGTGCCGGGC GGCCCCCTTC GGGGGGGNNN NNNNNNNGAG 
AGCGCTTCCC CAGAGCCGAG CCCACAGGAG CTCGAGAAGC TGGAAGTCCT CAACAACCAG 
GACTATGATG ACTACACGAG TATGACCGGT GAGTGGGCCT GGCCTGAGGA GGGGGATGCG 
AAGCAGCCCC CGCCCAACAA CAACATCCTG GGTCACGTGG TGCACCGGCT CAACGTGATC 
GTGGACCCCC CTAAGCCGGA GACCCCCCCG CCCTCGTTCC CCAGTTTCAC GCTGAAGGCC 
TCCGTCCTGG GAAAGGTGTT TGCAGGGAAA AGCGGCTGTC TGGCAAGGAT GGCGCAAGTC 
CACGGCGTCC ACGTCCTGTC GACCAGCATT CTGATCCAGG AGGCGCTGGA AGCGTTCCTG 
GCCGGAGAGA AGGTGCCGCA GGCGCCCCCG AAGGAGCCGC AGTATCCCGA GGGATCCGTC 
TGTCTCAGGA GGACTGCGAA GAAGAAGGAT GTTGCCGAGA AGGAGAAATC TTCACGCCCC 
GACTCAGGCC ATGTCCTCCA AAGCCCCCAG CAGGAAAATG CCAAGAGGAC AGCTTCCAAG 
TCTGAGGTAC TGACCTCACC TCCCCAGCTG TCGGCGAGGG CTCAGCACGG AGCCGCCCTG 
GACGAGGTCC TCGGCAAGGG GCAGGCCATC CCAGACCAGC TCCTCGTGGA CATTGTTCTG 
GAAGCCATCA GGCGGGTCCC TCCAGAGTCG GGCTGGATCC TCGATGGGTT CCCCATGGAC 
CTGGATCAGG CCAAGCTCCT GGAGAAGGCT CTCAGTGGGA TTGACCCAGA GAAGGTGGAA 
AGGAAGAAGA ATAACAAGAA GATCAACCTT GTCGTGGACC CCCATGCGAA GAAAGAGGCC 
CCACCCCCTC TACCTGCCCT GGACGTCGTT CTGCTTATGG ACTTGTCCGA TGACCTCGTT 
CTGGACCGTG CTGCAAAGAA AGCCAATGAG ATGGAAGGCA AGCTGGTGCA AATTCAGACT 
CTGGACGAGA TGTGCGCCTT GGTGGAGGCG GGATCTCCAC CAGAGCCGTG CCCCTCGACG 
CAAACTCCTG ACCTTCGGGA CAAGAGCCTG GAGATGGCGC AGGTCCAGCA CAGGATCGCA 
GCCTTCCACG ACACCTGGCC CAAGCTGGAG TGGTGGTACT CCAACAGGCA GAAGATCCTG 
GTGAAGGTGA ACGCGGACGA AGAGCCCGAC GCCGTCTTCA AGGAAGTGGA ATCCATCGTG 
TACAAGATCA TGGCGCCGCC GGCAGACAAG GGTGTAGACG CGACGCCTAC AGCCCAGACT 
GCTCAGGCCC CTGGTGTCAC TCCACCTCCT CTTGCAATGT CCTCCTCTCC AGCGGGACCC 
CCGGCGCAAA AATCCCCATC GGAGTCCCGA ATCCACTCCC CTGATGAAGG CGTCCCGGCC 
GATGCCGCCC CAGAAGCGCC CGTCCCCGAA GTCCCTCAAG GCAAGAGACC GAGCCCCAGA 
GCGCTCCCTA TGAGCCAGTC CACCGAGGTG GTCTACGTGG ACAAGCCGCT GCCCAAGGAA 
TTCCCGGAAT ACCTCGTTCC ATACTGGGAG AACACGTGCA CGTCGTATGT GACAAATATC 
AAAGCGGCGA TGCAGAACCT CCGCGCCGAG CGGGACCTGA TCATCCAGCA TCTCTATGAC 
ATCAGGCAGG AGTTCAAGCA CTACTTGATG AGACCGGACC TGAAGCAGGA GTTTGTGACC 
CAGTGGCAGC AGGACTACAA CAGCATCCCT GAGGACATGA GGGCTGACGA AGACACCAAG 
GCAGAGCTGC ACCAGCGCCT CAATGACCTG AGCGAGCGGC TGTGGGACAT CAGTGAGCGG 
CGCAAGGAGG AGGCGCTGCA GGAGCGGACC GTGCTCATGG GCGACGGCTG GCTGGAGGAC 
CACACGGCCA TGCTCATCAA CCACTTCTCC ACCCTCATGC AGGTGGAGGT GGACAGGTTC 
CAGGACACGG TGCATCTGCT GCGGGACTAT TACAGGGGCA TGTCCGGGCA GAACCTCCCG 
GAAACGCGGT TCGCGCACGT CCCCATGGTG GACGTCGCCG AAGACGCTCG GGACGAGGAC 
CCCACTGGCT CTGTGCAGCC TGAGCAGCAG GACACGAGCG CTGGAACAAG CGAAGAAGAG 
GAGAGCAAGA CTAAAATAAT CCCTCTGATC CCACGGAGAC CTCGGTCGGG AGAAGCCGCG 
CTGATAAAGC CGAACGCCTC GACGGACCCG CACCCGGACG AGCAGCTTTT GCATGACATT 
TGGCAGACGG CCGTGACCGC TGTTAATAAC CTGGTGTCGC AGGAGATGCC CCAGCTGGAG 
CCCGAGGAGG GTAAGGAGGC CCAGTACGTG GCGGAGCAGG GGCCGTCTCA GCCCGCGGCC 
CAGAGCGCCA AGGACAACGA GAAGCCTGGA AACACAAAAG GAGGTGTCCC AGAGCCCTCG 
GCCCCAGAGC AAGCGCCTAG CCCACCGCCC CAAGTCGAGG AGGACGTGGA GGTTGTCCGC 
AGAAAGACAG TGAAAGCCCA GATTCGCAAG GAGTTTGCCG CAGCACTCAG TCACGAGGAC 
AACGCGGTGA AAGTACGCCT GGAGCTTGTC CGAGCGCGAG CGCTGCGCGT ATTGAGGAGC 
CTCCACTGCC AAGCTGACCA AGCTTATAGT GCCATGGAGG AGTGGCTTCA CACCCGCTTT 
CAAGCAGAGA TGAACAGCAT CAACCAGTTG GTGGAGGTGT CGCGTCACCA CATCGAAGCG 
GCCGCCCAGA TCCAGCACGA GCTCGTGCTG GAGGGTCCCG ACTTCTTCAT TGATGGGGAT 
ACGCGCGTCG TAACCGCTTC NNNNNNNNNN TACGCCGCCG CCGTGCCCGC CCCCCTGGGA 
GCTCCCGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_018758460.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018613976.2 Scleropages formosus sperm flagellar 2 (spef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc135155
Clone ID Related Accession (Same CDS sequence)	XM_018758460.2
Accession Version	XM_018758460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041822.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018758460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
GTACTGACCT CACCTCCCCA GCTGTCGGCG AGGGCTCAGC ACGGAGCCGC CCTGGACGAG 
GTCCTCGGCA AGGGGCAGGC CATCCCAGAC CAGCTCCTCG TGGACATTGT TCTGGAAGCC 
ATCAGGCGGG TCCCTCCAGA GTCGGGCTGG ATCCTCGATG GGTTCCCCAT GGACCTGGAT 
CAGGCCAAGC TCCTGGAGAA GGCTCTCAGT GGGATTGACC CAGAGAAGGT GGAAAGGAAG 
AAGAATAACA AGAAGATCAA CCTTGTCGTG GACCCCCATG CGAAGAAAGA GGCCCCACCC 
CCTCTACCTG CCCTGGACGT CGTTCTGCTT ATGGACTTGT CCGATGACCT CGTTCTGGAC 
CGTGCTGCAA AGAAAGCCAA TGAGATGGAA GGCAAGCTGG TGCAAATTCA GACTCTGGAC 
GAGATGTGCG CCTTGGCGGA GGCGGGATCT CCACCAGAGC CGTGCCCCTC GACGCAAACT 
CCTGACCTTC GGGACAAGAG CCTGGAGATG GCGCAGGTCC AGCACAGGAT CGCAGCCTTC 
CACGACACCT GGCCCAAGCT GGAGTGGTGG TACTCCAACA GGCAGAAGAT CCTGGTGAAG 
GTGAACGCGG ACGAAGAGCC CGACGCCGTC TTCAAGGAAG TGGAATCCAT CGTGTACAAG 
ATCATGGCGC CGCCGGCAGA CAAGGGTGTA GACGCGACGC CTACAGCCCA GACTGCTCAG 
GCCCCTGGTG TCACTCCACC TCCTCTTGCA ATGTCCTCCT CTCCAGCGGG ACCCCCGGCG 
CAAAAATCCC CATCGGAGTC CCGAATCCAC TCCCCTGATG AAGGCGTCCC GGCCGATGCC 
GCCCCAGAAG CGCCCGTCCC CGAAGTCCCT CAAGGCAAGA GACCGAGCCC CAGAGCGCTC 
CCTATGAGCC AGTCCACCGA GGTGGTCTAC GTGGACAAGC CGCTGCCCAA GGAATTCCCG 
GAATACCTCG TTCCATACTG GGAGAACACG TGCACGTCGT ATGTGACAAA TATCAAAGCG 
GCGATGCAGA ACCTCCGCGC CGAGCGGGAC CTGATCATCC AGCATCTCTA TGACATCAGG 
CAGGAGTTCA AGCACTACTT GATGAGACCG GACCTGAAGC AGGAGTTTGT GACCCAGTGG 
CAGCAGGACT ACAACAGCAT CCCTGAGGAC ATGAGGGCTG ACGAAGACAC CAAGGCAGAG 
CTGCACCAGC GCCTCAATGA CCTGAGCGAG CGGCTGTGGG ACATCAGTGA GCGGCGCAAG 
GAGGAGGCGC TGCAGGAGCG GACCGTGCTC ATGGGCGACG GCTGGCTGGA GGACCACACG 
GCCATGCTCA TCAACCACTT CTCCACCCTC ATGCAGGTGG AGGTGGACAG GTTCCAGGAC 
ACGGTGCATC TGCTGCGGGA CTATTACAGG GGCATGTCCG GGCAGAACCT CCCGGAAACG 
CGGTTCGCGC ACGTCCCCAT GGTGGACGTC GCCGAAGACG CTCGGGACGA GGACCCCACT 
GGCTCTGTGC AGCCTGAGCA GCAGGACACG AGCGCTGGAA CAAGCGAAGA AGAGGAGAGC 
AAGACTAAAA TAATCCCTCT GATCCCACGG AGACCTCGGT CGGGGGAAGC CGCGCTGATA 
AAGCCGAACG CCTCGACGGA CCCGCACCCG GACGAGCAGC TTTTGCATGA CATTTGGCAG 
ACGGCCGTGA CCGCTGTTAA TAACCTGGTG TCGCAGGAGA TGCCCCAGCT GGAGCCCGAG 
GAGGGTAAGG AGGCCCAGTA CGTGGCGGAG CAGGGGCCGT CTCAGCCCGC GGCCCAGAGC 
GCCAAGGACA ACGAGAAGCC CGGAAACACA AAAGGAGGTG TCCCAGAGCC CTCGGCCCCA 
GAGCAAGCGC CTAGCCCACC GCCCCAAGTC GAGGAGGACG TGGAGGTTGT CCGCAGAAAG 
ACAGTGAAAG CCCAGATTCG CAAGGAGTTT GCCGCAGCAC TCAGTCACGA GGACAACGCG 
GTGAAAGTAC GCCTGGAGCT TGTCCGAGCG CGAGCGCTGC GCGTATTGAG GAGCCTCCAC 
TGCCAAGCTG ACCAAGCTTA TAGTGCCATG GAGGAGTGGC TTCACACCCG CTTTCAAGCA 
GAGATGAACA GCATCAACCA GTTGGTGGAG GTGTCGCGTC ACCACATCGA AGCGGCCGCC 
CAGATCCAGC ACGAGCTCGT GCTGGAGGGT CCCGACTTCT TCATTGATGG GGATACGCGC 
GTCGTACCCG CTTCGACGCC GCCGCCGTGC CCGCCCCCCT GGGAGCTCCC GGTTGACAAT 
GCCTTCACCA TCCGCCAGCT CGAGGCATTG TACGCGCAGC TTCGCAAAGT TGCCCCGGCA 
GGTCGGCTGT GCGCTAAAGA GCTCTCGGAG ATCCTGGAGG ATCTCGCCTC CCTGGACATG 
GGCAGCGATG CCTTGCCCGA ACTCTGGAGC AATCTGTCCG CGTCTCAGAT CCAGGACATG 
GTGTCACTCG TGAGTCAGGA TTCCGAGGTG CTGGACTGGC GACGCTTTCT GCTGAGCGCA 
GCGCTGCCGT GGCCCCTTCC CTCGCAGTCC CAGCTGCTGG AGGTTCTGGA CCGATTCCGA 
GCGACCGACT CCCAGGGAAC GGGCTTCGTC ACCGAGGAGC TATACCGACA GACGGAGCTC 
TGGTTCTCCG GGGAGAGCGG CGCGTCTCCT CCGGCCGACT CTTCCGAGCC CTCGCCGTTC 
GACCGCTTGT CGAACTTGAA GAAGTTTTTC TTCGCTCTTT TTGCGGACCT CCGCTCCCCG 
GAACCCCGGC TCCACTACGA GACCATGCTG CTGTACTTCG CCGCGCACCC GGACCGCGTC 
CGGGGTTGGG TTCGAGCCCT TGGCGTGGCA ACGGGCCGGC TCATCTACCA CCGGCCTCGC 
AGCGCCGTGC TCCTGAAGTC TGTGCCGCAC GTGGGGGACG AGGAGTCGTA TTACGAGCGG 
ACGGACGGCG AAGCGGAGCC CGGCGGGACA GAAGAAGGGA AGGGCGTGTC CATCGCTGCC 
CTCCTTAAAG TGGTCTGCCA TGGGGGGACC AGGACATCCA GCTACAACCG CTTTCACCCC 
AACTGGAGGA CTTGGGAAGA GTATGAGGAG GATTTCAGGC AGCTCTACGC AGAACTTGGA 
TTTGGACCAG AAGAGGAGGT TCCCTTTGCC ACGCTGTCCC AGCATCCTCT GATACAGAAC 
CTGATGGAAG ATGCCGTCCA GTACCGGCTC ACTGACATCC ATCAACTTGT TTTGGCCCAG 
GCTATAGAAG GAGAGTCGCC GTGCTTCCCG GTGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018613976.2
CDS	101..5359
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKVQEERRMKDIEKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQEELNRDERLLACLTRQSQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLLLGLPLYGESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKEKSSRPDSGHVLQSPQQENAKRTASKSEVLTSPPQLSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALAEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGGVPEPSAPEQAPSPPPQVEEDVEVVRRKTVKAQIRKEFAAALSHEDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVPASTPPPCPPPWELPVDNAFTIRQLEALYAQLRKVAPAGRLCAKELSEILEDLASLDMGSDALPELWSNLSASQIQDMVSLVSQDSEVLDWRRFLLSAALPWPLPSQSQLLEVLDRFRATDSQGTGFVTEELYRQTELWFSGESGASPPADSSEPSPFDRLSNLKKFFFALFADLRSPEPRLHYETMLLYFAAHPDRVRGWVRALGVATGRLIYHRPRSAVLLKSVPHVGDEESYYERTDGEAEPGGTEEGKGVSIAALLKVVCHGGTRTSSYNRFHPNWRTWEEYEEDFRQLYAELGFGPEEEVPFATLSQHPLIQNLMEDAVQYRLTDIHQLVLAQAIEGESPCFPVP

Target ORF information:

RefSeq Version	XM_018758460.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018758460.2

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
GTACTGACCT CACCTCCCCA GCTGTCGGCG AGGGCTCAGC ACGGAGCCGC CCTGGACGAG 
GTCCTCGGCA AGGGGCAGGC CATCCCAGAC CAGCTCCTCG TGGACATTGT TCTGGAAGCC 
ATCAGGCGGG TCCCTCCAGA GTCGGGCTGG ATCCTCGATG GGTTCCCCAT GGACCTGGAT 
CAGGCCAAGC TCCTGGAGAA GGCTCTCAGT GGGATTGACC CAGAGAAGGT GGAAAGGAAG 
AAGAATAACA AGAAGATCAA CCTTGTCGTG GACCCCCATG CGAAGAAAGA GGCCCCACCC 
CCTCTACCTG CCCTGGACGT CGTTCTGCTT ATGGACTTGT CCGATGACCT CGTTCTGGAC 
CGTGCTGCAA AGAAAGCCAA TGAGATGGAA GGCAAGCTGG TGCAAATTCA GACTCTGGAC 
GAGATGTGCG CCTTGGCGGA GGCGGGATCT CCACCAGAGC CGTGCCCCTC GACGCAAACT 
CCTGACCTTC GGGACAAGAG CCTGGAGATG GCGCAGGTCC AGCACAGGAT CGCAGCCTTC 
CACGACACCT GGCCCAAGCT GGAGTGGTGG TACTCCAACA GGCAGAAGAT CCTGGTGAAG 
GTGAACGCGG ACGAAGAGCC CGACGCCGTC TTCAAGGAAG TGGAATCCAT CGTGTACAAG 
ATCATGGCGC CGCCGGCAGA CAAGGGTGTA GACGCGACGC CTACAGCCCA GACTGCTCAG 
GCCCCTGGTG TCACTCCACC TCCTCTTGCA ATGTCCTCCT CTCCAGCGGG ACCCCCGGCG 
CAAAAATCCC CATCGGAGTC CCGAATCCAC TCCCCTGATG AAGGCGTCCC GGCCGATGCC 
GCCCCAGAAG CGCCCGTCCC CGAAGTCCCT CAAGGCAAGA GACCGAGCCC CAGAGCGCTC 
CCTATGAGCC AGTCCACCGA GGTGGTCTAC GTGGACAAGC CGCTGCCCAA GGAATTCCCG 
GAATACCTCG TTCCATACTG GGAGAACACG TGCACGTCGT ATGTGACAAA TATCAAAGCG 
GCGATGCAGA ACCTCCGCGC CGAGCGGGAC CTGATCATCC AGCATCTCTA TGACATCAGG 
CAGGAGTTCA AGCACTACTT GATGAGACCG GACCTGAAGC AGGAGTTTGT GACCCAGTGG 
CAGCAGGACT ACAACAGCAT CCCTGAGGAC ATGAGGGCTG ACGAAGACAC CAAGGCAGAG 
CTGCACCAGC GCCTCAATGA CCTGAGCGAG CGGCTGTGGG ACATCAGTGA GCGGCGCAAG 
GAGGAGGCGC TGCAGGAGCG GACCGTGCTC ATGGGCGACG GCTGGCTGGA GGACCACACG 
GCCATGCTCA TCAACCACTT CTCCACCCTC ATGCAGGTGG AGGTGGACAG GTTCCAGGAC 
ACGGTGCATC TGCTGCGGGA CTATTACAGG GGCATGTCCG GGCAGAACCT CCCGGAAACG 
CGGTTCGCGC ACGTCCCCAT GGTGGACGTC GCCGAAGACG CTCGGGACGA GGACCCCACT 
GGCTCTGTGC AGCCTGAGCA GCAGGACACG AGCGCTGGAA CAAGCGAAGA AGAGGAGAGC 
AAGACTAAAA TAATCCCTCT GATCCCACGG AGACCTCGGT CGGGGGAAGC CGCGCTGATA 
AAGCCGAACG CCTCGACGGA CCCGCACCCG GACGAGCAGC TTTTGCATGA CATTTGGCAG 
ACGGCCGTGA CCGCTGTTAA TAACCTGGTG TCGCAGGAGA TGCCCCAGCT GGAGCCCGAG 
GAGGGTAAGG AGGCCCAGTA CGTGGCGGAG CAGGGGCCGT CTCAGCCCGC GGCCCAGAGC 
GCCAAGGACA ACGAGAAGCC CGGAAACACA AAAGGAGGTG TCCCAGAGCC CTCGGCCCCA 
GAGCAAGCGC CTAGCCCACC GCCCCAAGTC GAGGAGGACG TGGAGGTTGT CCGCAGAAAG 
ACAGTGAAAG CCCAGATTCG CAAGGAGTTT GCCGCAGCAC TCAGTCACGA GGACAACGCG 
GTGAAAGTAC GCCTGGAGCT TGTCCGAGCG CGAGCGCTGC GCGTATTGAG GAGCCTCCAC 
TGCCAAGCTG ACCAAGCTTA TAGTGCCATG GAGGAGTGGC TTCACACCCG CTTTCAAGCA 
GAGATGAACA GCATCAACCA GTTGGTGGAG GTGTCGCGTC ACCACATCGA AGCGGCCGCC 
CAGATCCAGC ACGAGCTCGT GCTGGAGGGT CCCGACTTCT TCATTGATGG GGATACGCGC 
GTCGTACCCG CTTCGACGCC GCCGCCGTGC CCGCCCCCCT GGGAGCTCCC GGTTGACAAT 
GCCTTCACCA TCCGCCAGCT CGAGGCATTG TACGCGCAGC TTCGCAAAGT TGCCCCGGCA 
GGTCGGCTGT GCGCTAAAGA GCTCTCGGAG ATCCTGGAGG ATCTCGCCTC CCTGGACATG 
GGCAGCGATG CCTTGCCCGA ACTCTGGAGC AATCTGTCCG CGTCTCAGAT CCAGGACATG 
GTGTCACTCG TGAGTCAGGA TTCCGAGGTG CTGGACTGGC GACGCTTTCT GCTGAGCGCA 
GCGCTGCCGT GGCCCCTTCC CTCGCAGTCC CAGCTGCTGG AGGTTCTGGA CCGATTCCGA 
GCGACCGACT CCCAGGGAAC GGGCTTCGTC ACCGAGGAGC TATACCGACA GACGGAGCTC 
TGGTTCTCCG GGGAGAGCGG CGCGTCTCCT CCGGCCGACT CTTCCGAGCC CTCGCCGTTC 
GACCGCTTGT CGAACTTGAA GAAGTTTTTC TTCGCTCTTT TTGCGGACCT CCGCTCCCCG 
GAACCCCGGC TCCACTACGA GACCATGCTG CTGTACTTCG CCGCGCACCC GGACCGCGTC 
CGGGGTTGGG TTCGAGCCCT TGGCGTGGCA ACGGGCCGGC TCATCTACCA CCGGCCTCGC 
AGCGCCGTGC TCCTGAAGTC TGTGCCGCAC GTGGGGGACG AGGAGTCGTA TTACGAGCGG 
ACGGACGGCG AAGCGGAGCC CGGCGGGACA GAAGAAGGGA AGGGCGTGTC CATCGCTGCC 
CTCCTTAAAG TGGTCTGCCA TGGGGGGACC AGGACATCCA GCTACAACCG CTTTCACCCC 
AACTGGAGGA CTTGGGAAGA GTATGAGGAG GATTTCAGGC AGCTCTACGC AGAACTTGGA 
TTTGGACCAG AAGAGGAGGT TCCCTTTGCC ACGCTGTCCC AGCATCCTCT GATACAGAAC 
CTGATGGAAG ATGCCGTCCA GTACCGGCTC ACTGACATCC ATCAACTTGT TTTGGCCCAG 
GCTATAGAAG GAGAGTCGCC GTGCTTCCCG GTGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_029259498.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029115331.1 Scleropages formosus sperm flagellar 2 (spef2), transcript variant X2, mRNA.

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CloneID	OSc135156
Clone ID Related Accession (Same CDS sequence)	XM_029259498.1
Accession Version	XM_029259498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041822.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
CTGTCGGCGA GGGCTCAGCA CGGAGCCGCC CTGGACGAGG TCCTCGGCAA GGGGCAGGCC 
ATCCCAGACC AGCTCCTCGT GGACATTGTT CTGGAAGCCA TCAGGCGGGT CCCTCCAGAG 
TCGGGCTGGA TCCTCGATGG GTTCCCCATG GACCTGGATC AGGCCAAGCT CCTGGAGAAG 
GCTCTCAGTG GGATTGACCC AGAGAAGGTG GAAAGGAAGA AGAATAACAA GAAGATCAAC 
CTTGTCGTGG ACCCCCATGC GAAGAAAGAG GCCCCACCCC CTCTACCTGC CCTGGACGTC 
GTTCTGCTTA TGGACTTGTC CGATGACCTC GTTCTGGACC GTGCTGCAAA GAAAGCCAAT 
GAGATGGAAG GCAAGCTGGT GCAAATTCAG ACTCTGGACG AGATGTGCGC CTTGGCGGAG 
GCGGGATCTC CACCAGAGCC GTGCCCCTCG ACGCAAACTC CTGACCTTCG GGACAAGAGC 
CTGGAGATGG CGCAGGTCCA GCACAGGATC GCAGCCTTCC ACGACACCTG GCCCAAGCTG 
GAGTGGTGGT ACTCCAACAG GCAGAAGATC CTGGTGAAGG TGAACGCGGA CGAAGAGCCC 
GACGCCGTCT TCAAGGAAGT GGAATCCATC GTGTACAAGA TCATGGCGCC GCCGGCAGAC 
AAGGGTGTAG ACGCGACGCC TACAGCCCAG ACTGCTCAGG CCCCTGGTGT CACTCCACCT 
CCTCTTGCAA TGTCCTCCTC TCCAGCGGGA CCCCCGGCGC AAAAATCCCC ATCGGAGTCC 
CGAATCCACT CCCCTGATGA AGGCGTCCCG GCCGATGCCG CCCCAGAAGC GCCCGTCCCC 
GAAGTCCCTC AAGGCAAGAG ACCGAGCCCC AGAGCGCTCC CTATGAGCCA GTCCACCGAG 
GTGGTCTACG TGGACAAGCC GCTGCCCAAG GAATTCCCGG AATACCTCGT TCCATACTGG 
GAGAACACGT GCACGTCGTA TGTGACAAAT ATCAAAGCGG CGATGCAGAA CCTCCGCGCC 
GAGCGGGACC TGATCATCCA GCATCTCTAT GACATCAGGC AGGAGTTCAA GCACTACTTG 
ATGAGACCGG ACCTGAAGCA GGAGTTTGTG ACCCAGTGGC AGCAGGACTA CAACAGCATC 
CCTGAGGACA TGAGGGCTGA CGAAGACACC AAGGCAGAGC TGCACCAGCG CCTCAATGAC 
CTGAGCGAGC GGCTGTGGGA CATCAGTGAG CGGCGCAAGG AGGAGGCGCT GCAGGAGCGG 
ACCGTGCTCA TGGGCGACGG CTGGCTGGAG GACCACACGG CCATGCTCAT CAACCACTTC 
TCCACCCTCA TGCAGGTGGA GGTGGACAGG TTCCAGGACA CGGTGCATCT GCTGCGGGAC 
TATTACAGGG GCATGTCCGG GCAGAACCTC CCGGAAACGC GGTTCGCGCA CGTCCCCATG 
GTGGACGTCG CCGAAGACGC TCGGGACGAG GACCCCACTG GCTCTGTGCA GCCTGAGCAG 
CAGGACACGA GCGCTGGAAC AAGCGAAGAA GAGGAGAGCA AGACTAAAAT AATCCCTCTG 
ATCCCACGGA GACCTCGGTC GGGGGAAGCC GCGCTGATAA AGCCGAACGC CTCGACGGAC 
CCGCACCCGG ACGAGCAGCT TTTGCATGAC ATTTGGCAGA CGGCCGTGAC CGCTGTTAAT 
AACCTGGTGT CGCAGGAGAT GCCCCAGCTG GAGCCCGAGG AGGGTAAGGA GGCCCAGTAC 
GTGGCGGAGC AGGGGCCGTC TCAGCCCGCG GCCCAGAGCG CCAAGGACAA CGAGAAGCCC 
GGAAACACAA AAGGAGGTGT CCCAGAGCCC TCGGCCCCAG AGCAAGCGCC TAGCCCACCG 
CCCCAAGTCG AGGAGGACGT GGAGGTTGTC CGCAGAAAGA CAGTGAAAGC CCAGATTCGC 
AAGGAGTTTG CCGCAGCACT CAGTCACGAG GACAACGCGG TGAAAGTACG CCTGGAGCTT 
GTCCGAGCGC GAGCGCTGCG CGTATTGAGG AGCCTCCACT GCCAAGCTGA CCAAGCTTAT 
AGTGCCATGG AGGAGTGGCT TCACACCCGC TTTCAAGCAG AGATGAACAG CATCAACCAG 
TTGGTGGAGG TGTCGCGTCA CCACATCGAA GCGGCCGCCC AGATCCAGCA CGAGCTCGTG 
CTGGAGGGTC CCGACTTCTT CATTGATGGG GATACGCGCG TCGTACCCGC TTCGACGCCG 
CCGCCGTGCC CGCCCCCCTG GGAGCTCCCG GTTGACAATG CCTTCACCAT CCGCCAGCTC 
GAGGCATTGT ACGCGCAGCT TCGCAAAGTT GCCCCGGCAG GTCGGCTGTG CGCTAAAGAG 
CTCTCGGAGA TCCTGGAGGA TCTCGCCTCC CTGGACATGG GCAGCGATGC CTTGCCCGAA 
CTCTGGAGCA ATCTGTCCGC GTCTCAGATC CAGGACATGG TGTCACTCGT GAGTCAGGAT 
TCCGAGGTGC TGGACTGGCG ACGCTTTCTG CTGAGCGCAG CGCTGCCGTG GCCCCTTCCC 
TCGCAGTCCC AGCTGCTGGA GGTTCTGGAC CGATTCCGAG CGACCGACTC CCAGGGAACG 
GGCTTCGTCA CCGAGGAGCT ATACCGACAG ACGGAGCTCT GGTTCTCCGG GGAGAGCGGC 
GCGTCTCCTC CGGCCGACTC TTCCGAGCCC TCGCCGTTCG ACCGCTTGTC GAACTTGAAG 
AAGTTTTTCT TCGCTCTTTT TGCGGACCTC CGCTCCCCGG AACCCCGGCT CCACTACGAG 
ACCATGCTGC TGTACTTCGC CGCGCACCCG GACCGCGTCC GGGGTTGGGT TCGAGCCCTT 
GGCGTGGCAA CGGGCCGGCT CATCTACCAC CGGCCTCGCA GCGCCGTGCT CCTGAAGTCT 
GTGCCGCACG TGGGGGACGA GGAGTCGTAT TACGAGCGGA CGGACGGCGA AGCGGAGCCC 
GGCGGGACAG AAGAAGGGAA GGGCGTGTCC ATCGCTGCCC TCCTTAAAGT GGTCTGCCAT 
GGGGGGACCA GGACATCCAG CTACAACCGC TTTCACCCCA ACTGGAGGAC TTGGGAAGAG 
TATGAGGAGG ATTTCAGGCA GCTCTACGCA GAACTTGGAT TTGGACCAGA AGAGGAGGTT 
CCCTTTGCCA CGCTGTCCCA GCATCCTCTG ATACAGAACC TGATGGAAGA TGCCGTCCAG 
TACCGGCTCA CTGACATCCA TCAACTTGTT TTGGCCCAGG CTATAGAAGG AGAGTCGCCG 
TGCTTCCCGG TGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029115331.1
CDS	101..5338
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKVQEERRMKDIEKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQEELNRDERLLACLTRQSQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLLLGLPLYGESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKEKSSRPDSGHVLQSPQQENAKRTASKSELSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALAEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGGVPEPSAPEQAPSPPPQVEEDVEVVRRKTVKAQIRKEFAAALSHEDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVPASTPPPCPPPWELPVDNAFTIRQLEALYAQLRKVAPAGRLCAKELSEILEDLASLDMGSDALPELWSNLSASQIQDMVSLVSQDSEVLDWRRFLLSAALPWPLPSQSQLLEVLDRFRATDSQGTGFVTEELYRQTELWFSGESGASPPADSSEPSPFDRLSNLKKFFFALFADLRSPEPRLHYETMLLYFAAHPDRVRGWVRALGVATGRLIYHRPRSAVLLKSVPHVGDEESYYERTDGEAEPGGTEEGKGVSIAALLKVVCHGGTRTSSYNRFHPNWRTWEEYEEDFRQLYAELGFGPEEEVPFATLSQHPLIQNLMEDAVQYRLTDIHQLVLAQAIEGESPCFPVP

Target ORF information:

RefSeq Version	XM_029259498.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029259498.1

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
CTGTCGGCGA GGGCTCAGCA CGGAGCCGCC CTGGACGAGG TCCTCGGCAA GGGGCAGGCC 
ATCCCAGACC AGCTCCTCGT GGACATTGTT CTGGAAGCCA TCAGGCGGGT CCCTCCAGAG 
TCGGGCTGGA TCCTCGATGG GTTCCCCATG GACCTGGATC AGGCCAAGCT CCTGGAGAAG 
GCTCTCAGTG GGATTGACCC AGAGAAGGTG GAAAGGAAGA AGAATAACAA GAAGATCAAC 
CTTGTCGTGG ACCCCCATGC GAAGAAAGAG GCCCCACCCC CTCTACCTGC CCTGGACGTC 
GTTCTGCTTA TGGACTTGTC CGATGACCTC GTTCTGGACC GTGCTGCAAA GAAAGCCAAT 
GAGATGGAAG GCAAGCTGGT GCAAATTCAG ACTCTGGACG AGATGTGCGC CTTGGCGGAG 
GCGGGATCTC CACCAGAGCC GTGCCCCTCG ACGCAAACTC CTGACCTTCG GGACAAGAGC 
CTGGAGATGG CGCAGGTCCA GCACAGGATC GCAGCCTTCC ACGACACCTG GCCCAAGCTG 
GAGTGGTGGT ACTCCAACAG GCAGAAGATC CTGGTGAAGG TGAACGCGGA CGAAGAGCCC 
GACGCCGTCT TCAAGGAAGT GGAATCCATC GTGTACAAGA TCATGGCGCC GCCGGCAGAC 
AAGGGTGTAG ACGCGACGCC TACAGCCCAG ACTGCTCAGG CCCCTGGTGT CACTCCACCT 
CCTCTTGCAA TGTCCTCCTC TCCAGCGGGA CCCCCGGCGC AAAAATCCCC ATCGGAGTCC 
CGAATCCACT CCCCTGATGA AGGCGTCCCG GCCGATGCCG CCCCAGAAGC GCCCGTCCCC 
GAAGTCCCTC AAGGCAAGAG ACCGAGCCCC AGAGCGCTCC CTATGAGCCA GTCCACCGAG 
GTGGTCTACG TGGACAAGCC GCTGCCCAAG GAATTCCCGG AATACCTCGT TCCATACTGG 
GAGAACACGT GCACGTCGTA TGTGACAAAT ATCAAAGCGG CGATGCAGAA CCTCCGCGCC 
GAGCGGGACC TGATCATCCA GCATCTCTAT GACATCAGGC AGGAGTTCAA GCACTACTTG 
ATGAGACCGG ACCTGAAGCA GGAGTTTGTG ACCCAGTGGC AGCAGGACTA CAACAGCATC 
CCTGAGGACA TGAGGGCTGA CGAAGACACC AAGGCAGAGC TGCACCAGCG CCTCAATGAC 
CTGAGCGAGC GGCTGTGGGA CATCAGTGAG CGGCGCAAGG AGGAGGCGCT GCAGGAGCGG 
ACCGTGCTCA TGGGCGACGG CTGGCTGGAG GACCACACGG CCATGCTCAT CAACCACTTC 
TCCACCCTCA TGCAGGTGGA GGTGGACAGG TTCCAGGACA CGGTGCATCT GCTGCGGGAC 
TATTACAGGG GCATGTCCGG GCAGAACCTC CCGGAAACGC GGTTCGCGCA CGTCCCCATG 
GTGGACGTCG CCGAAGACGC TCGGGACGAG GACCCCACTG GCTCTGTGCA GCCTGAGCAG 
CAGGACACGA GCGCTGGAAC AAGCGAAGAA GAGGAGAGCA AGACTAAAAT AATCCCTCTG 
ATCCCACGGA GACCTCGGTC GGGGGAAGCC GCGCTGATAA AGCCGAACGC CTCGACGGAC 
CCGCACCCGG ACGAGCAGCT TTTGCATGAC ATTTGGCAGA CGGCCGTGAC CGCTGTTAAT 
AACCTGGTGT CGCAGGAGAT GCCCCAGCTG GAGCCCGAGG AGGGTAAGGA GGCCCAGTAC 
GTGGCGGAGC AGGGGCCGTC TCAGCCCGCG GCCCAGAGCG CCAAGGACAA CGAGAAGCCC 
GGAAACACAA AAGGAGGTGT CCCAGAGCCC TCGGCCCCAG AGCAAGCGCC TAGCCCACCG 
CCCCAAGTCG AGGAGGACGT GGAGGTTGTC CGCAGAAAGA CAGTGAAAGC CCAGATTCGC 
AAGGAGTTTG CCGCAGCACT CAGTCACGAG GACAACGCGG TGAAAGTACG CCTGGAGCTT 
GTCCGAGCGC GAGCGCTGCG CGTATTGAGG AGCCTCCACT GCCAAGCTGA CCAAGCTTAT 
AGTGCCATGG AGGAGTGGCT TCACACCCGC TTTCAAGCAG AGATGAACAG CATCAACCAG 
TTGGTGGAGG TGTCGCGTCA CCACATCGAA GCGGCCGCCC AGATCCAGCA CGAGCTCGTG 
CTGGAGGGTC CCGACTTCTT CATTGATGGG GATACGCGCG TCGTACCCGC TTCGACGCCG 
CCGCCGTGCC CGCCCCCCTG GGAGCTCCCG GTTGACAATG CCTTCACCAT CCGCCAGCTC 
GAGGCATTGT ACGCGCAGCT TCGCAAAGTT GCCCCGGCAG GTCGGCTGTG CGCTAAAGAG 
CTCTCGGAGA TCCTGGAGGA TCTCGCCTCC CTGGACATGG GCAGCGATGC CTTGCCCGAA 
CTCTGGAGCA ATCTGTCCGC GTCTCAGATC CAGGACATGG TGTCACTCGT GAGTCAGGAT 
TCCGAGGTGC TGGACTGGCG ACGCTTTCTG CTGAGCGCAG CGCTGCCGTG GCCCCTTCCC 
TCGCAGTCCC AGCTGCTGGA GGTTCTGGAC CGATTCCGAG CGACCGACTC CCAGGGAACG 
GGCTTCGTCA CCGAGGAGCT ATACCGACAG ACGGAGCTCT GGTTCTCCGG GGAGAGCGGC 
GCGTCTCCTC CGGCCGACTC TTCCGAGCCC TCGCCGTTCG ACCGCTTGTC GAACTTGAAG 
AAGTTTTTCT TCGCTCTTTT TGCGGACCTC CGCTCCCCGG AACCCCGGCT CCACTACGAG 
ACCATGCTGC TGTACTTCGC CGCGCACCCG GACCGCGTCC GGGGTTGGGT TCGAGCCCTT 
GGCGTGGCAA CGGGCCGGCT CATCTACCAC CGGCCTCGCA GCGCCGTGCT CCTGAAGTCT 
GTGCCGCACG TGGGGGACGA GGAGTCGTAT TACGAGCGGA CGGACGGCGA AGCGGAGCCC 
GGCGGGACAG AAGAAGGGAA GGGCGTGTCC ATCGCTGCCC TCCTTAAAGT GGTCTGCCAT 
GGGGGGACCA GGACATCCAG CTACAACCGC TTTCACCCCA ACTGGAGGAC TTGGGAAGAG 
TATGAGGAGG ATTTCAGGCA GCTCTACGCA GAACTTGGAT TTGGACCAGA AGAGGAGGTT 
CCCTTTGCCA CGCTGTCCCA GCATCCTCTG ATACAGAACC TGATGGAAGA TGCCGTCCAG 
TACCGGCTCA CTGACATCCA TCAACTTGTT TTGGCCCAGG CTATAGAAGG AGAGTCGCCG 
TGCTTCCCGG TGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_029259499.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029115332.1 Scleropages formosus sperm flagellar 2 (spef2), transcript variant X3, mRNA.

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CloneID	OSc135157
Clone ID Related Accession (Same CDS sequence)	XM_029259499.1
Accession Version	XM_029259499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041822.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGC TCCGTCTGGC CCGGAAAAGG CGCAACGAGA TGATGGTTAA GATCCAAAAA 
GCCACTGTCC AGATCCCCAG GCCTCCCAGA AAGCAAGTCC TGAGGACCCT GGAGCACCAG 
CATCACAAAC AGCAGGAAGC TCAGGATTTT CAGAGGGACA TTGAGGACTT TGAGAGGAAC 
ATGAAAAAGC AACTTCCTGC CAGCGCTTTG GCTCTGAGCA CGCCCTTCAC GTTTTCCGTC 
AAGACGGATG CCGCCCTGCG GCCCGAGCAG CTGGCCCAGG CCAACGACGA GTACATCCAG 
AGGATCCGAC AGCGGCTGGA GGAGGACGCC GCTGCCCGGC AGCAACGGGA GAAACGCAGA 
AGCCGCATCC TGGTGTTGCA GCAGGAGGCT CAAGAGGAGC AGGAGGAGTT GAACCGCGAT 
GAACGGCTGC TGGCGTGTTT GACCCGTCAG TCACAGCAGG AGAGGCGACT CGCCGTGCAG 
CTCATGCAGG TGCGGCAGCA GAAGGAGTTC CTGAGACAGA ACCGGATCTT CCGGGAGAAA 
CAGTACCAGG AGCAACGCCT GCGGGAGTTC CAGGAGGCCC TGGACCGGAA GGCGGCTCTG 
GCCCAGCAGA CCCAATTGGA GCACGAGCAG GAGACCCAGA GGGAGACGGA GCTGCACCGG 
CAGATCACGG CCCAGCGAGC GCAAGCTCGG TACCGCAGCC ACTTCGATAC CTGCAGAGAG 
GTCCTGGACC AGATACTGAA CCTGGTCACC AAGGTTGGGG AGTACCGGCT CCTCATGGAC 
AACCTGATTC CAGTCAAGCT GATGAGGGAG TGGAAGGAGC TGCTGCTGCT CGGGCTGCCG 
CTCTACGGTG AGAGCGCTTC CCCAGAGCCG AGCCCACAGG AGCTCGAGAA GCTGGAAGTC 
CTCAACAACC AGGACTATGA TGACTACACG AGTATGACCG GTGAGTGGGC CTGGCCTGAG 
GAGGGGGATG CGAAGCAGCC CCCGCCCAAC AACAACATCC TGGGTCACGT GGTGCACCGG 
CTCAACGTGA TCGTGGACCC CCCTAAGCCG GAGACCCCCC CGCCCTCGTT CCCCAGTTTC 
ACGCTGAAGG CCTCCGTCCT GGGAAAGGTG TTTGCAGGGA AAAGCGGCTG TCTGGCAAGG 
ATGGCGCAAG TCCACGGCGT CCACGTCCTG TCGACCAGCA TTCTGATCCA GGAGGCGCTG 
GAAGCGTTCC TGGCCGGAGA GAAGGTGCCG CAGGCGCCCC CGAAGGAGCC GCAGTATCCC 
GAGGGATCCG TCTGTCTCAG GAGGACTGCG AAGAAGAAGG ATGTTGCCGA GAAGGAGAAG 
GAGAAATCTT CACGCCCCGA CTCAGGCCAT GTCCTCCAAA GCCCCCAGCA GGAAAATGCC 
AAGAGGACAG CTTCCAAGTC TGAGGTACTG ACCTCACCTC CCCAGCTGTC GGCGAGGGCT 
CAGCACGGAG CCGCCCTGGA CGAGGTCCTC GGCAAGGGGC AGGCCATCCC AGACCAGCTC 
CTCGTGGACA TTGTTCTGGA AGCCATCAGG CGGGTCCCTC CAGAGTCGGG CTGGATCCTC 
GATGGGTTCC CCATGGACCT GGATCAGGCC AAGCTCCTGG AGAAGGCTCT CAGTGGGATT 
GACCCAGAGA AGGTGGAAAG GAAGAAGAAT AACAAGAAGA TCAACCTTGT CGTGGACCCC 
CATGCGAAGA AAGAGGCCCC ACCCCCTCTA CCTGCCCTGG ACGTCGTTCT GCTTATGGAC 
TTGTCCGATG ACCTCGTTCT GGACCGTGCT GCAAAGAAAG CCAATGAGAT GGAAGGCAAG 
CTGGTGCAAA TTCAGACTCT GGACGAGATG TGCGCCTTGG CGGAGGCGGG ATCTCCACCA 
GAGCCGTGCC CCTCGACGCA AACTCCTGAC CTTCGGGACA AGAGCCTGGA GATGGCGCAG 
GTCCAGCACA GGATCGCAGC CTTCCACGAC ACCTGGCCCA AGCTGGAGTG GTGGTACTCC 
AACAGGCAGA AGATCCTGGT GAAGGTGAAC GCGGACGAAG AGCCCGACGC CGTCTTCAAG 
GAAGTGGAAT CCATCGTGTA CAAGATCATG GCGCCGCCGG CAGACAAGGG TGTAGACGCG 
ACGCCTACAG CCCAGACTGC TCAGGCCCCT GGTGTCACTC CACCTCCTCT TGCAATGTCC 
TCCTCTCCAG CGGGACCCCC GGCGCAAAAA TCCCCATCGG AGTCCCGAAT CCACTCCCCT 
GATGAAGGCG TCCCGGCCGA TGCCGCCCCA GAAGCGCCCG TCCCCGAAGT CCCTCAAGGC 
AAGAGACCGA GCCCCAGAGC GCTCCCTATG AGCCAGTCCA CCGAGGTGGT CTACGTGGAC 
AAGCCGCTGC CCAAGGAATT CCCGGAATAC CTCGTTCCAT ACTGGGAGAA CACGTGCACG 
TCGTATGTGA CAAATATCAA AGCGGCGATG CAGAACCTCC GCGCCGAGCG GGACCTGATC 
ATCCAGCATC TCTATGACAT CAGGCAGGAG TTCAAGCACT ACTTGATGAG ACCGGACCTG 
AAGCAGGAGT TTGTGACCCA GTGGCAGCAG GACTACAACA GCATCCCTGA GGACATGAGG 
GCTGACGAAG ACACCAAGGC AGAGCTGCAC CAGCGCCTCA ATGACCTGAG CGAGCGGCTG 
TGGGACATCA GTGAGCGGCG CAAGGAGGAG GCGCTGCAGG AGCGGACCGT GCTCATGGGC 
GACGGCTGGC TGGAGGACCA CACGGCCATG CTCATCAACC ACTTCTCCAC CCTCATGCAG 
GTGGAGGTGG ACAGGTTCCA GGACACGGTG CATCTGCTGC GGGACTATTA CAGGGGCATG 
TCCGGGCAGA ACCTCCCGGA AACGCGGTTC GCGCACGTCC CCATGGTGGA CGTCGCCGAA 
GACGCTCGGG ACGAGGACCC CACTGGCTCT GTGCAGCCTG AGCAGCAGGA CACGAGCGCT 
GGAACAAGCG AAGAAGAGGA GAGCAAGACT AAAATAATCC CTCTGATCCC ACGGAGACCT 
CGGTCGGGGG AAGCCGCGCT GATAAAGCCG AACGCCTCGA CGGACCCGCA CCCGGACGAG 
CAGCTTTTGC ATGACATTTG GCAGACGGCC GTGACCGCTG TTAATAACCT GGTGTCGCAG 
GAGATGCCCC AGCTGGAGCC CGAGGAGGGT AAGGAGGCCC AGTACGTGGC GGAGCAGGGG 
CCGTCTCAGC CCGCGGCCCA GAGCGCCAAG GACAACGAGA AGCCCGGAAA CACAAAAGGA 
GGTGTCCCAG AGCCCTCGGC CCCAGAGCAA GCGCCTAGCC CACCGCCCCA AGTCGAGGAG 
GACGTGGAGG TTGTCCGCAG AAAGACAGTG AAAGCCCAGA TTCGCAAGGA GTTTGCCGCA 
GCACTCAGTC ACGAGGACAA CGCGGTGAAA GTACGCCTGG AGCTTGTCCG AGCGCGAGCG 
CTGCGCGTAT TGAGGAGCCT CCACTGCCAA GCTGACCAAG CTTATAGTGC CATGGAGGAG 
TGGCTTCACA CCCGCTTTCA AGCAGAGATG AACAGCATCA ACCAGTTGGT GGAGGTGTCG 
CGTCACCACA TCGAAGCGGC CGCCCAGATC CAGCACGAGC TCGTGCTGGA GGGTCCCGAC 
TTCTTCATTG ATGGGGATAC GCGCGTCGTA CCCGCTTCGA CGCCGCCGCC GTGCCCGCCC 
CCCTGGGAGC TCCCGGTTGA CAATGCCTTC ACCATCCGCC AGCTCGAGGC ATTGTACGCG 
CAGCTTCGCA AAGTTGCCCC GGCAGGTCGG CTGTGCGCTA AAGAGCTCTC GGAGATCCTG 
GAGGATCTCG CCTCCCTGGA CATGGGCAGC GATGCCTTGC CCGAACTCTG GAGCAATCTG 
TCCGCGTCTC AGATCCAGGA CATGGTGTCA CTCGTGAGTC AGGATTCCGA GGTGCTGGAC 
TGGCGACGCT TTCTGCTGAG CGCAGCGCTG CCGTGGCCCC TTCCCTCGCA GTCCCAGCTG 
CTGGAGGTTC TGGACCGATT CCGAGCGACC GACTCCCAGG GAACGGGCTT CGTCACCGAG 
GAGCTATACC GACAGACGGA GCTCTGGTTC TCCGGGGAGA GCGGCGCGTC TCCTCCGGCC 
GACTCTTCCG AGCCCTCGCC GTTCGACCGC TTGTCGAACT TGAAGAAGTT TTTCTTCGCT 
CTTTTTGCGG ACCTCCGCTC CCCGGAACCC CGGCTCCACT ACGAGACCAT GCTGCTGTAC 
TTCGCCGCGC ACCCGGACCG CGTCCGGGGT TGGGTTCGAG CCCTTGGCGT GGCAACGGGC 
CGGCTCATCT ACCACCGGCC TCGCAGCGCC GTGCTCCTGA AGTCTGTGCC GCACGTGGGG 
GACGAGGAGT CGTATTACGA GCGGACGGAC GGCGAAGCGG AGCCCGGCGG GACAGAAGAA 
GGGAAGGGCG TGTCCATCGC TGCCCTCCTT AAAGTGGTCT GCCATGGGGG GACCAGGACA 
TCCAGCTACA ACCGCTTTCA CCCCAACTGG AGGACTTGGG AAGAGTATGA GGAGGATTTC 
AGGCAGCTCT ACGCAGAACT TGGATTTGGA CCAGAAGAGG AGGTTCCCTT TGCCACGCTG 
TCCCAGCATC CTCTGATACA GAACCTGATG GAAGATGCCG TCCAGTACCG GCTCACTGAC 
ATCCATCAAC TTGTTTTGGC CCAGGCTATA GAAGGAGAGT CGCCGTGCTT CCCGGTGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029115332.1
CDS	101..5323
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQEELNRDERLLACLTRQSQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLLLGLPLYGESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKEKSSRPDSGHVLQSPQQENAKRTASKSEVLTSPPQLSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALAEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGGVPEPSAPEQAPSPPPQVEEDVEVVRRKTVKAQIRKEFAAALSHEDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVPASTPPPCPPPWELPVDNAFTIRQLEALYAQLRKVAPAGRLCAKELSEILEDLASLDMGSDALPELWSNLSASQIQDMVSLVSQDSEVLDWRRFLLSAALPWPLPSQSQLLEVLDRFRATDSQGTGFVTEELYRQTELWFSGESGASPPADSSEPSPFDRLSNLKKFFFALFADLRSPEPRLHYETMLLYFAAHPDRVRGWVRALGVATGRLIYHRPRSAVLLKSVPHVGDEESYYERTDGEAEPGGTEEGKGVSIAALLKVVCHGGTRTSSYNRFHPNWRTWEEYEEDFRQLYAELGFGPEEEVPFATLSQHPLIQNLMEDAVQYRLTDIHQLVLAQAIEGESPCFPVP

Target ORF information:

RefSeq Version	XM_029259499.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029259499.1

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGC TCCGTCTGGC CCGGAAAAGG CGCAACGAGA TGATGGTTAA GATCCAAAAA 
GCCACTGTCC AGATCCCCAG GCCTCCCAGA AAGCAAGTCC TGAGGACCCT GGAGCACCAG 
CATCACAAAC AGCAGGAAGC TCAGGATTTT CAGAGGGACA TTGAGGACTT TGAGAGGAAC 
ATGAAAAAGC AACTTCCTGC CAGCGCTTTG GCTCTGAGCA CGCCCTTCAC GTTTTCCGTC 
AAGACGGATG CCGCCCTGCG GCCCGAGCAG CTGGCCCAGG CCAACGACGA GTACATCCAG 
AGGATCCGAC AGCGGCTGGA GGAGGACGCC GCTGCCCGGC AGCAACGGGA GAAACGCAGA 
AGCCGCATCC TGGTGTTGCA GCAGGAGGCT CAAGAGGAGC AGGAGGAGTT GAACCGCGAT 
GAACGGCTGC TGGCGTGTTT GACCCGTCAG TCACAGCAGG AGAGGCGACT CGCCGTGCAG 
CTCATGCAGG TGCGGCAGCA GAAGGAGTTC CTGAGACAGA ACCGGATCTT CCGGGAGAAA 
CAGTACCAGG AGCAACGCCT GCGGGAGTTC CAGGAGGCCC TGGACCGGAA GGCGGCTCTG 
GCCCAGCAGA CCCAATTGGA GCACGAGCAG GAGACCCAGA GGGAGACGGA GCTGCACCGG 
CAGATCACGG CCCAGCGAGC GCAAGCTCGG TACCGCAGCC ACTTCGATAC CTGCAGAGAG 
GTCCTGGACC AGATACTGAA CCTGGTCACC AAGGTTGGGG AGTACCGGCT CCTCATGGAC 
AACCTGATTC CAGTCAAGCT GATGAGGGAG TGGAAGGAGC TGCTGCTGCT CGGGCTGCCG 
CTCTACGGTG AGAGCGCTTC CCCAGAGCCG AGCCCACAGG AGCTCGAGAA GCTGGAAGTC 
CTCAACAACC AGGACTATGA TGACTACACG AGTATGACCG GTGAGTGGGC CTGGCCTGAG 
GAGGGGGATG CGAAGCAGCC CCCGCCCAAC AACAACATCC TGGGTCACGT GGTGCACCGG 
CTCAACGTGA TCGTGGACCC CCCTAAGCCG GAGACCCCCC CGCCCTCGTT CCCCAGTTTC 
ACGCTGAAGG CCTCCGTCCT GGGAAAGGTG TTTGCAGGGA AAAGCGGCTG TCTGGCAAGG 
ATGGCGCAAG TCCACGGCGT CCACGTCCTG TCGACCAGCA TTCTGATCCA GGAGGCGCTG 
GAAGCGTTCC TGGCCGGAGA GAAGGTGCCG CAGGCGCCCC CGAAGGAGCC GCAGTATCCC 
GAGGGATCCG TCTGTCTCAG GAGGACTGCG AAGAAGAAGG ATGTTGCCGA GAAGGAGAAG 
GAGAAATCTT CACGCCCCGA CTCAGGCCAT GTCCTCCAAA GCCCCCAGCA GGAAAATGCC 
AAGAGGACAG CTTCCAAGTC TGAGGTACTG ACCTCACCTC CCCAGCTGTC GGCGAGGGCT 
CAGCACGGAG CCGCCCTGGA CGAGGTCCTC GGCAAGGGGC AGGCCATCCC AGACCAGCTC 
CTCGTGGACA TTGTTCTGGA AGCCATCAGG CGGGTCCCTC CAGAGTCGGG CTGGATCCTC 
GATGGGTTCC CCATGGACCT GGATCAGGCC AAGCTCCTGG AGAAGGCTCT CAGTGGGATT 
GACCCAGAGA AGGTGGAAAG GAAGAAGAAT AACAAGAAGA TCAACCTTGT CGTGGACCCC 
CATGCGAAGA AAGAGGCCCC ACCCCCTCTA CCTGCCCTGG ACGTCGTTCT GCTTATGGAC 
TTGTCCGATG ACCTCGTTCT GGACCGTGCT GCAAAGAAAG CCAATGAGAT GGAAGGCAAG 
CTGGTGCAAA TTCAGACTCT GGACGAGATG TGCGCCTTGG CGGAGGCGGG ATCTCCACCA 
GAGCCGTGCC CCTCGACGCA AACTCCTGAC CTTCGGGACA AGAGCCTGGA GATGGCGCAG 
GTCCAGCACA GGATCGCAGC CTTCCACGAC ACCTGGCCCA AGCTGGAGTG GTGGTACTCC 
AACAGGCAGA AGATCCTGGT GAAGGTGAAC GCGGACGAAG AGCCCGACGC CGTCTTCAAG 
GAAGTGGAAT CCATCGTGTA CAAGATCATG GCGCCGCCGG CAGACAAGGG TGTAGACGCG 
ACGCCTACAG CCCAGACTGC TCAGGCCCCT GGTGTCACTC CACCTCCTCT TGCAATGTCC 
TCCTCTCCAG CGGGACCCCC GGCGCAAAAA TCCCCATCGG AGTCCCGAAT CCACTCCCCT 
GATGAAGGCG TCCCGGCCGA TGCCGCCCCA GAAGCGCCCG TCCCCGAAGT CCCTCAAGGC 
AAGAGACCGA GCCCCAGAGC GCTCCCTATG AGCCAGTCCA CCGAGGTGGT CTACGTGGAC 
AAGCCGCTGC CCAAGGAATT CCCGGAATAC CTCGTTCCAT ACTGGGAGAA CACGTGCACG 
TCGTATGTGA CAAATATCAA AGCGGCGATG CAGAACCTCC GCGCCGAGCG GGACCTGATC 
ATCCAGCATC TCTATGACAT CAGGCAGGAG TTCAAGCACT ACTTGATGAG ACCGGACCTG 
AAGCAGGAGT TTGTGACCCA GTGGCAGCAG GACTACAACA GCATCCCTGA GGACATGAGG 
GCTGACGAAG ACACCAAGGC AGAGCTGCAC CAGCGCCTCA ATGACCTGAG CGAGCGGCTG 
TGGGACATCA GTGAGCGGCG CAAGGAGGAG GCGCTGCAGG AGCGGACCGT GCTCATGGGC 
GACGGCTGGC TGGAGGACCA CACGGCCATG CTCATCAACC ACTTCTCCAC CCTCATGCAG 
GTGGAGGTGG ACAGGTTCCA GGACACGGTG CATCTGCTGC GGGACTATTA CAGGGGCATG 
TCCGGGCAGA ACCTCCCGGA AACGCGGTTC GCGCACGTCC CCATGGTGGA CGTCGCCGAA 
GACGCTCGGG ACGAGGACCC CACTGGCTCT GTGCAGCCTG AGCAGCAGGA CACGAGCGCT 
GGAACAAGCG AAGAAGAGGA GAGCAAGACT AAAATAATCC CTCTGATCCC ACGGAGACCT 
CGGTCGGGGG AAGCCGCGCT GATAAAGCCG AACGCCTCGA CGGACCCGCA CCCGGACGAG 
CAGCTTTTGC ATGACATTTG GCAGACGGCC GTGACCGCTG TTAATAACCT GGTGTCGCAG 
GAGATGCCCC AGCTGGAGCC CGAGGAGGGT AAGGAGGCCC AGTACGTGGC GGAGCAGGGG 
CCGTCTCAGC CCGCGGCCCA GAGCGCCAAG GACAACGAGA AGCCCGGAAA CACAAAAGGA 
GGTGTCCCAG AGCCCTCGGC CCCAGAGCAA GCGCCTAGCC CACCGCCCCA AGTCGAGGAG 
GACGTGGAGG TTGTCCGCAG AAAGACAGTG AAAGCCCAGA TTCGCAAGGA GTTTGCCGCA 
GCACTCAGTC ACGAGGACAA CGCGGTGAAA GTACGCCTGG AGCTTGTCCG AGCGCGAGCG 
CTGCGCGTAT TGAGGAGCCT CCACTGCCAA GCTGACCAAG CTTATAGTGC CATGGAGGAG 
TGGCTTCACA CCCGCTTTCA AGCAGAGATG AACAGCATCA ACCAGTTGGT GGAGGTGTCG 
CGTCACCACA TCGAAGCGGC CGCCCAGATC CAGCACGAGC TCGTGCTGGA GGGTCCCGAC 
TTCTTCATTG ATGGGGATAC GCGCGTCGTA CCCGCTTCGA CGCCGCCGCC GTGCCCGCCC 
CCCTGGGAGC TCCCGGTTGA CAATGCCTTC ACCATCCGCC AGCTCGAGGC ATTGTACGCG 
CAGCTTCGCA AAGTTGCCCC GGCAGGTCGG CTGTGCGCTA AAGAGCTCTC GGAGATCCTG 
GAGGATCTCG CCTCCCTGGA CATGGGCAGC GATGCCTTGC CCGAACTCTG GAGCAATCTG 
TCCGCGTCTC AGATCCAGGA CATGGTGTCA CTCGTGAGTC AGGATTCCGA GGTGCTGGAC 
TGGCGACGCT TTCTGCTGAG CGCAGCGCTG CCGTGGCCCC TTCCCTCGCA GTCCCAGCTG 
CTGGAGGTTC TGGACCGATT CCGAGCGACC GACTCCCAGG GAACGGGCTT CGTCACCGAG 
GAGCTATACC GACAGACGGA GCTCTGGTTC TCCGGGGAGA GCGGCGCGTC TCCTCCGGCC 
GACTCTTCCG AGCCCTCGCC GTTCGACCGC TTGTCGAACT TGAAGAAGTT TTTCTTCGCT 
CTTTTTGCGG ACCTCCGCTC CCCGGAACCC CGGCTCCACT ACGAGACCAT GCTGCTGTAC 
TTCGCCGCGC ACCCGGACCG CGTCCGGGGT TGGGTTCGAG CCCTTGGCGT GGCAACGGGC 
CGGCTCATCT ACCACCGGCC TCGCAGCGCC GTGCTCCTGA AGTCTGTGCC GCACGTGGGG 
GACGAGGAGT CGTATTACGA GCGGACGGAC GGCGAAGCGG AGCCCGGCGG GACAGAAGAA 
GGGAAGGGCG TGTCCATCGC TGCCCTCCTT AAAGTGGTCT GCCATGGGGG GACCAGGACA 
TCCAGCTACA ACCGCTTTCA CCCCAACTGG AGGACTTGGG AAGAGTATGA GGAGGATTTC 
AGGCAGCTCT ACGCAGAACT TGGATTTGGA CCAGAAGAGG AGGTTCCCTT TGCCACGCTG 
TCCCAGCATC CTCTGATACA GAACCTGATG GAAGATGCCG TCCAGTACCG GCTCACTGAC 
ATCCATCAAC TTGTTTTGGC CCAGGCTATA GAAGGAGAGT CGCCGTGCTT CCCGGTGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_029259500.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029115333.1 Scleropages formosus sperm flagellar 2 (spef2), transcript variant X4, mRNA.

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CloneID	OSc135158
Clone ID Related Accession (Same CDS sequence)	XM_029259500.1
Accession Version	XM_029259500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041822.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GTCACAGCAG GAGAGGCGAC TCGCCGTGCA GCTCATGCAG 
GTGCGGCAGC AGAAGGAGTT CCTGAGACAG AACCGGATCT TCCGGGAGAA ACAGTACCAG 
GAGCAACGCC TGCGGGAGTT CCAGGAGGCC CTGGACCGGA AGGCGGCTCT GGCCCAGCAG 
ACCCAATTGG AGCACGAGCA GGAGACCCAG AGGGAGACGG AGCTGCACCG GCAGATCACG 
GCCCAGCGAG CGCAAGCTCG GTACCGCAGC CACTTCGATA CCTGCAGAGA GGTCCTGGAC 
CAGATACTGA ACCTGGTCAC CAAGGTTGGG GAGTACCGGC TCCTCATGGA CAACCTGATT 
CCAGTCAAGC TGATGAGGGA GTGGAAGGAG CTGCTGCTGC TCGGGCTGCC GCTCTACGGT 
GAGAGCGCTT CCCCAGAGCC GAGCCCACAG GAGCTCGAGA AGCTGGAAGT CCTCAACAAC 
CAGGACTATG ATGACTACAC GAGTATGACC GGTGAGTGGG CCTGGCCTGA GGAGGGGGAT 
GCGAAGCAGC CCCCGCCCAA CAACAACATC CTGGGTCACG TGGTGCACCG GCTCAACGTG 
ATCGTGGACC CCCCTAAGCC GGAGACCCCC CCGCCCTCGT TCCCCAGTTT CACGCTGAAG 
GCCTCCGTCC TGGGAAAGGT GTTTGCAGGG AAAAGCGGCT GTCTGGCAAG GATGGCGCAA 
GTCCACGGCG TCCACGTCCT GTCGACCAGC ATTCTGATCC AGGAGGCGCT GGAAGCGTTC 
CTGGCCGGAG AGAAGGTGCC GCAGGCGCCC CCGAAGGAGC CGCAGTATCC CGAGGGATCC 
GTCTGTCTCA GGAGGACTGC GAAGAAGAAG GATGTTGCCG AGAAGGAGAA GGAGAAATCT 
TCACGCCCCG ACTCAGGCCA TGTCCTCCAA AGCCCCCAGC AGGAAAATGC CAAGAGGACA 
GCTTCCAAGT CTGAGGTACT GACCTCACCT CCCCAGCTGT CGGCGAGGGC TCAGCACGGA 
GCCGCCCTGG ACGAGGTCCT CGGCAAGGGG CAGGCCATCC CAGACCAGCT CCTCGTGGAC 
ATTGTTCTGG AAGCCATCAG GCGGGTCCCT CCAGAGTCGG GCTGGATCCT CGATGGGTTC 
CCCATGGACC TGGATCAGGC CAAGCTCCTG GAGAAGGCTC TCAGTGGGAT TGACCCAGAG 
AAGGTGGAAA GGAAGAAGAA TAACAAGAAG ATCAACCTTG TCGTGGACCC CCATGCGAAG 
AAAGAGGCCC CACCCCCTCT ACCTGCCCTG GACGTCGTTC TGCTTATGGA CTTGTCCGAT 
GACCTCGTTC TGGACCGTGC TGCAAAGAAA GCCAATGAGA TGGAAGGCAA GCTGGTGCAA 
ATTCAGACTC TGGACGAGAT GTGCGCCTTG GCGGAGGCGG GATCTCCACC AGAGCCGTGC 
CCCTCGACGC AAACTCCTGA CCTTCGGGAC AAGAGCCTGG AGATGGCGCA GGTCCAGCAC 
AGGATCGCAG CCTTCCACGA CACCTGGCCC AAGCTGGAGT GGTGGTACTC CAACAGGCAG 
AAGATCCTGG TGAAGGTGAA CGCGGACGAA GAGCCCGACG CCGTCTTCAA GGAAGTGGAA 
TCCATCGTGT ACAAGATCAT GGCGCCGCCG GCAGACAAGG GTGTAGACGC GACGCCTACA 
GCCCAGACTG CTCAGGCCCC TGGTGTCACT CCACCTCCTC TTGCAATGTC CTCCTCTCCA 
GCGGGACCCC CGGCGCAAAA ATCCCCATCG GAGTCCCGAA TCCACTCCCC TGATGAAGGC 
GTCCCGGCCG ATGCCGCCCC AGAAGCGCCC GTCCCCGAAG TCCCTCAAGG CAAGAGACCG 
AGCCCCAGAG CGCTCCCTAT GAGCCAGTCC ACCGAGGTGG TCTACGTGGA CAAGCCGCTG 
CCCAAGGAAT TCCCGGAATA CCTCGTTCCA TACTGGGAGA ACACGTGCAC GTCGTATGTG 
ACAAATATCA AAGCGGCGAT GCAGAACCTC CGCGCCGAGC GGGACCTGAT CATCCAGCAT 
CTCTATGACA TCAGGCAGGA GTTCAAGCAC TACTTGATGA GACCGGACCT GAAGCAGGAG 
TTTGTGACCC AGTGGCAGCA GGACTACAAC AGCATCCCTG AGGACATGAG GGCTGACGAA 
GACACCAAGG CAGAGCTGCA CCAGCGCCTC AATGACCTGA GCGAGCGGCT GTGGGACATC 
AGTGAGCGGC GCAAGGAGGA GGCGCTGCAG GAGCGGACCG TGCTCATGGG CGACGGCTGG 
CTGGAGGACC ACACGGCCAT GCTCATCAAC CACTTCTCCA CCCTCATGCA GGTGGAGGTG 
GACAGGTTCC AGGACACGGT GCATCTGCTG CGGGACTATT ACAGGGGCAT GTCCGGGCAG 
AACCTCCCGG AAACGCGGTT CGCGCACGTC CCCATGGTGG ACGTCGCCGA AGACGCTCGG 
GACGAGGACC CCACTGGCTC TGTGCAGCCT GAGCAGCAGG ACACGAGCGC TGGAACAAGC 
GAAGAAGAGG AGAGCAAGAC TAAAATAATC CCTCTGATCC CACGGAGACC TCGGTCGGGG 
GAAGCCGCGC TGATAAAGCC GAACGCCTCG ACGGACCCGC ACCCGGACGA GCAGCTTTTG 
CATGACATTT GGCAGACGGC CGTGACCGCT GTTAATAACC TGGTGTCGCA GGAGATGCCC 
CAGCTGGAGC CCGAGGAGGG TAAGGAGGCC CAGTACGTGG CGGAGCAGGG GCCGTCTCAG 
CCCGCGGCCC AGAGCGCCAA GGACAACGAG AAGCCCGGAA ACACAAAAGG AGGTGTCCCA 
GAGCCCTCGG CCCCAGAGCA AGCGCCTAGC CCACCGCCCC AAGTCGAGGA GGACGTGGAG 
GTTGTCCGCA GAAAGACAGT GAAAGCCCAG ATTCGCAAGG AGTTTGCCGC AGCACTCAGT 
CACGAGGACA ACGCGGTGAA AGTACGCCTG GAGCTTGTCC GAGCGCGAGC GCTGCGCGTA 
TTGAGGAGCC TCCACTGCCA AGCTGACCAA GCTTATAGTG CCATGGAGGA GTGGCTTCAC 
ACCCGCTTTC AAGCAGAGAT GAACAGCATC AACCAGTTGG TGGAGGTGTC GCGTCACCAC 
ATCGAAGCGG CCGCCCAGAT CCAGCACGAG CTCGTGCTGG AGGGTCCCGA CTTCTTCATT 
GATGGGGATA CGCGCGTCGT ACCCGCTTCG ACGCCGCCGC CGTGCCCGCC CCCCTGGGAG 
CTCCCGGTTG ACAATGCCTT CACCATCCGC CAGCTCGAGG CATTGTACGC GCAGCTTCGC 
AAAGTTGCCC CGGCAGGTCG GCTGTGCGCT AAAGAGCTCT CGGAGATCCT GGAGGATCTC 
GCCTCCCTGG ACATGGGCAG CGATGCCTTG CCCGAACTCT GGAGCAATCT GTCCGCGTCT 
CAGATCCAGG ACATGGTGTC ACTCGTGAGT CAGGATTCCG AGGTGCTGGA CTGGCGACGC 
TTTCTGCTGA GCGCAGCGCT GCCGTGGCCC CTTCCCTCGC AGTCCCAGCT GCTGGAGGTT 
CTGGACCGAT TCCGAGCGAC CGACTCCCAG GGAACGGGCT TCGTCACCGA GGAGCTATAC 
CGACAGACGG AGCTCTGGTT CTCCGGGGAG AGCGGCGCGT CTCCTCCGGC CGACTCTTCC 
GAGCCCTCGC CGTTCGACCG CTTGTCGAAC TTGAAGAAGT TTTTCTTCGC TCTTTTTGCG 
GACCTCCGCT CCCCGGAACC CCGGCTCCAC TACGAGACCA TGCTGCTGTA CTTCGCCGCG 
CACCCGGACC GCGTCCGGGG TTGGGTTCGA GCCCTTGGCG TGGCAACGGG CCGGCTCATC 
TACCACCGGC CTCGCAGCGC CGTGCTCCTG AAGTCTGTGC CGCACGTGGG GGACGAGGAG 
TCGTATTACG AGCGGACGGA CGGCGAAGCG GAGCCCGGCG GGACAGAAGA AGGGAAGGGC 
GTGTCCATCG CTGCCCTCCT TAAAGTGGTC TGCCATGGGG GGACCAGGAC ATCCAGCTAC 
AACCGCTTTC ACCCCAACTG GAGGACTTGG GAAGAGTATG AGGAGGATTT CAGGCAGCTC 
TACGCAGAAC TTGGATTTGG ACCAGAAGAG GAGGTTCCCT TTGCCACGCT GTCCCAGCAT 
CCTCTGATAC AGAACCTGAT GGAAGATGCC GTCCAGTACC GGCTCACTGA CATCCATCAA 
CTTGTTTTGG CCCAGGCTAT AGAAGGAGAG TCGCCGTGCT TCCCGGTGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029115333.1
CDS	101..5314
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKVQEERRMKDIEKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQESQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLLLGLPLYGESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKEKSSRPDSGHVLQSPQQENAKRTASKSEVLTSPPQLSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALAEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGGVPEPSAPEQAPSPPPQVEEDVEVVRRKTVKAQIRKEFAAALSHEDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVPASTPPPCPPPWELPVDNAFTIRQLEALYAQLRKVAPAGRLCAKELSEILEDLASLDMGSDALPELWSNLSASQIQDMVSLVSQDSEVLDWRRFLLSAALPWPLPSQSQLLEVLDRFRATDSQGTGFVTEELYRQTELWFSGESGASPPADSSEPSPFDRLSNLKKFFFALFADLRSPEPRLHYETMLLYFAAHPDRVRGWVRALGVATGRLIYHRPRSAVLLKSVPHVGDEESYYERTDGEAEPGGTEEGKGVSIAALLKVVCHGGTRTSSYNRFHPNWRTWEEYEEDFRQLYAELGFGPEEEVPFATLSQHPLIQNLMEDAVQYRLTDIHQLVLAQAIEGESPCFPVP

Target ORF information:

RefSeq Version	XM_029259500.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029259500.1

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GTCACAGCAG GAGAGGCGAC TCGCCGTGCA GCTCATGCAG 
GTGCGGCAGC AGAAGGAGTT CCTGAGACAG AACCGGATCT TCCGGGAGAA ACAGTACCAG 
GAGCAACGCC TGCGGGAGTT CCAGGAGGCC CTGGACCGGA AGGCGGCTCT GGCCCAGCAG 
ACCCAATTGG AGCACGAGCA GGAGACCCAG AGGGAGACGG AGCTGCACCG GCAGATCACG 
GCCCAGCGAG CGCAAGCTCG GTACCGCAGC CACTTCGATA CCTGCAGAGA GGTCCTGGAC 
CAGATACTGA ACCTGGTCAC CAAGGTTGGG GAGTACCGGC TCCTCATGGA CAACCTGATT 
CCAGTCAAGC TGATGAGGGA GTGGAAGGAG CTGCTGCTGC TCGGGCTGCC GCTCTACGGT 
GAGAGCGCTT CCCCAGAGCC GAGCCCACAG GAGCTCGAGA AGCTGGAAGT CCTCAACAAC 
CAGGACTATG ATGACTACAC GAGTATGACC GGTGAGTGGG CCTGGCCTGA GGAGGGGGAT 
GCGAAGCAGC CCCCGCCCAA CAACAACATC CTGGGTCACG TGGTGCACCG GCTCAACGTG 
ATCGTGGACC CCCCTAAGCC GGAGACCCCC CCGCCCTCGT TCCCCAGTTT CACGCTGAAG 
GCCTCCGTCC TGGGAAAGGT GTTTGCAGGG AAAAGCGGCT GTCTGGCAAG GATGGCGCAA 
GTCCACGGCG TCCACGTCCT GTCGACCAGC ATTCTGATCC AGGAGGCGCT GGAAGCGTTC 
CTGGCCGGAG AGAAGGTGCC GCAGGCGCCC CCGAAGGAGC CGCAGTATCC CGAGGGATCC 
GTCTGTCTCA GGAGGACTGC GAAGAAGAAG GATGTTGCCG AGAAGGAGAA GGAGAAATCT 
TCACGCCCCG ACTCAGGCCA TGTCCTCCAA AGCCCCCAGC AGGAAAATGC CAAGAGGACA 
GCTTCCAAGT CTGAGGTACT GACCTCACCT CCCCAGCTGT CGGCGAGGGC TCAGCACGGA 
GCCGCCCTGG ACGAGGTCCT CGGCAAGGGG CAGGCCATCC CAGACCAGCT CCTCGTGGAC 
ATTGTTCTGG AAGCCATCAG GCGGGTCCCT CCAGAGTCGG GCTGGATCCT CGATGGGTTC 
CCCATGGACC TGGATCAGGC CAAGCTCCTG GAGAAGGCTC TCAGTGGGAT TGACCCAGAG 
AAGGTGGAAA GGAAGAAGAA TAACAAGAAG ATCAACCTTG TCGTGGACCC CCATGCGAAG 
AAAGAGGCCC CACCCCCTCT ACCTGCCCTG GACGTCGTTC TGCTTATGGA CTTGTCCGAT 
GACCTCGTTC TGGACCGTGC TGCAAAGAAA GCCAATGAGA TGGAAGGCAA GCTGGTGCAA 
ATTCAGACTC TGGACGAGAT GTGCGCCTTG GCGGAGGCGG GATCTCCACC AGAGCCGTGC 
CCCTCGACGC AAACTCCTGA CCTTCGGGAC AAGAGCCTGG AGATGGCGCA GGTCCAGCAC 
AGGATCGCAG CCTTCCACGA CACCTGGCCC AAGCTGGAGT GGTGGTACTC CAACAGGCAG 
AAGATCCTGG TGAAGGTGAA CGCGGACGAA GAGCCCGACG CCGTCTTCAA GGAAGTGGAA 
TCCATCGTGT ACAAGATCAT GGCGCCGCCG GCAGACAAGG GTGTAGACGC GACGCCTACA 
GCCCAGACTG CTCAGGCCCC TGGTGTCACT CCACCTCCTC TTGCAATGTC CTCCTCTCCA 
GCGGGACCCC CGGCGCAAAA ATCCCCATCG GAGTCCCGAA TCCACTCCCC TGATGAAGGC 
GTCCCGGCCG ATGCCGCCCC AGAAGCGCCC GTCCCCGAAG TCCCTCAAGG CAAGAGACCG 
AGCCCCAGAG CGCTCCCTAT GAGCCAGTCC ACCGAGGTGG TCTACGTGGA CAAGCCGCTG 
CCCAAGGAAT TCCCGGAATA CCTCGTTCCA TACTGGGAGA ACACGTGCAC GTCGTATGTG 
ACAAATATCA AAGCGGCGAT GCAGAACCTC CGCGCCGAGC GGGACCTGAT CATCCAGCAT 
CTCTATGACA TCAGGCAGGA GTTCAAGCAC TACTTGATGA GACCGGACCT GAAGCAGGAG 
TTTGTGACCC AGTGGCAGCA GGACTACAAC AGCATCCCTG AGGACATGAG GGCTGACGAA 
GACACCAAGG CAGAGCTGCA CCAGCGCCTC AATGACCTGA GCGAGCGGCT GTGGGACATC 
AGTGAGCGGC GCAAGGAGGA GGCGCTGCAG GAGCGGACCG TGCTCATGGG CGACGGCTGG 
CTGGAGGACC ACACGGCCAT GCTCATCAAC CACTTCTCCA CCCTCATGCA GGTGGAGGTG 
GACAGGTTCC AGGACACGGT GCATCTGCTG CGGGACTATT ACAGGGGCAT GTCCGGGCAG 
AACCTCCCGG AAACGCGGTT CGCGCACGTC CCCATGGTGG ACGTCGCCGA AGACGCTCGG 
GACGAGGACC CCACTGGCTC TGTGCAGCCT GAGCAGCAGG ACACGAGCGC TGGAACAAGC 
GAAGAAGAGG AGAGCAAGAC TAAAATAATC CCTCTGATCC CACGGAGACC TCGGTCGGGG 
GAAGCCGCGC TGATAAAGCC GAACGCCTCG ACGGACCCGC ACCCGGACGA GCAGCTTTTG 
CATGACATTT GGCAGACGGC CGTGACCGCT GTTAATAACC TGGTGTCGCA GGAGATGCCC 
CAGCTGGAGC CCGAGGAGGG TAAGGAGGCC CAGTACGTGG CGGAGCAGGG GCCGTCTCAG 
CCCGCGGCCC AGAGCGCCAA GGACAACGAG AAGCCCGGAA ACACAAAAGG AGGTGTCCCA 
GAGCCCTCGG CCCCAGAGCA AGCGCCTAGC CCACCGCCCC AAGTCGAGGA GGACGTGGAG 
GTTGTCCGCA GAAAGACAGT GAAAGCCCAG ATTCGCAAGG AGTTTGCCGC AGCACTCAGT 
CACGAGGACA ACGCGGTGAA AGTACGCCTG GAGCTTGTCC GAGCGCGAGC GCTGCGCGTA 
TTGAGGAGCC TCCACTGCCA AGCTGACCAA GCTTATAGTG CCATGGAGGA GTGGCTTCAC 
ACCCGCTTTC AAGCAGAGAT GAACAGCATC AACCAGTTGG TGGAGGTGTC GCGTCACCAC 
ATCGAAGCGG CCGCCCAGAT CCAGCACGAG CTCGTGCTGG AGGGTCCCGA CTTCTTCATT 
GATGGGGATA CGCGCGTCGT ACCCGCTTCG ACGCCGCCGC CGTGCCCGCC CCCCTGGGAG 
CTCCCGGTTG ACAATGCCTT CACCATCCGC CAGCTCGAGG CATTGTACGC GCAGCTTCGC 
AAAGTTGCCC CGGCAGGTCG GCTGTGCGCT AAAGAGCTCT CGGAGATCCT GGAGGATCTC 
GCCTCCCTGG ACATGGGCAG CGATGCCTTG CCCGAACTCT GGAGCAATCT GTCCGCGTCT 
CAGATCCAGG ACATGGTGTC ACTCGTGAGT CAGGATTCCG AGGTGCTGGA CTGGCGACGC 
TTTCTGCTGA GCGCAGCGCT GCCGTGGCCC CTTCCCTCGC AGTCCCAGCT GCTGGAGGTT 
CTGGACCGAT TCCGAGCGAC CGACTCCCAG GGAACGGGCT TCGTCACCGA GGAGCTATAC 
CGACAGACGG AGCTCTGGTT CTCCGGGGAG AGCGGCGCGT CTCCTCCGGC CGACTCTTCC 
GAGCCCTCGC CGTTCGACCG CTTGTCGAAC TTGAAGAAGT TTTTCTTCGC TCTTTTTGCG 
GACCTCCGCT CCCCGGAACC CCGGCTCCAC TACGAGACCA TGCTGCTGTA CTTCGCCGCG 
CACCCGGACC GCGTCCGGGG TTGGGTTCGA GCCCTTGGCG TGGCAACGGG CCGGCTCATC 
TACCACCGGC CTCGCAGCGC CGTGCTCCTG AAGTCTGTGC CGCACGTGGG GGACGAGGAG 
TCGTATTACG AGCGGACGGA CGGCGAAGCG GAGCCCGGCG GGACAGAAGA AGGGAAGGGC 
GTGTCCATCG CTGCCCTCCT TAAAGTGGTC TGCCATGGGG GGACCAGGAC ATCCAGCTAC 
AACCGCTTTC ACCCCAACTG GAGGACTTGG GAAGAGTATG AGGAGGATTT CAGGCAGCTC 
TACGCAGAAC TTGGATTTGG ACCAGAAGAG GAGGTTCCCT TTGCCACGCT GTCCCAGCAT 
CCTCTGATAC AGAACCTGAT GGAAGATGCC GTCCAGTACC GGCTCACTGA CATCCATCAA 
CTTGTTTTGG CCCAGGCTAT AGAAGGAGAG TCGCCGTGCT TCCCGGTGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_029259501.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029115334.1 Scleropages formosus sperm flagellar 2 (spef2), transcript variant X5, mRNA.

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CloneID	OSc135159
Clone ID Related Accession (Same CDS sequence)	XM_029259501.1
Accession Version	XM_029259501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041822.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
GTACTGACCT CACCTCCCCA GCTGTCGGCG AGGGCTCAGC ACGGAGCCGC CCTGGACGAG 
GTCCTCGGCA AGGGGCAGGC CATCCCAGAC CAGCTCCTCG TGGACATTGT TCTGGAAGCC 
ATCAGGCGGG TCCCTCCAGA GTCGGGCTGG ATCCTCGATG GGTTCCCCAT GGACCTGGAT 
CAGGCCAAGC TCCTGGAGAA GGCTCTCAGT GGGATTGACC CAGAGAAGGT GGAAAGGAAG 
AAGAATAACA AGAAGATCAA CCTTGTCGTG GACCCCCATG CGAAGAAAGA GGCCCCACCC 
CCTCTACCTG CCCTGGACGT CGTTCTGCTT ATGGACTTGT CCGATGACCT CGTTCTGGAC 
CGTGCTGCAA AGAAAGCCAA TGAGATGGAA GGCAAGCTGG TGCAAATTCA GACTCTGGAC 
GAGATGTGCG CCTTGGCGGA GGCGGGATCT CCACCAGAGC CGTGCCCCTC GACGCAAACT 
CCTGACCTTC GGGACAAGAG CCTGGAGATG GCGCAGGTCC AGCACAGGAT CGCAGCCTTC 
CACGACACCT GGCCCAAGCT GGAGTGGTGG TACTCCAACA GGCAGAAGAT CCTGGTGAAG 
GTGAACGCGG ACGAAGAGCC CGACGCCGTC TTCAAGGAAG TGGAATCCAT CGTGTACAAG 
ATCATGGCGC CGCCGGCAGA CAAGGGTGTA GACGCGACGC CTACAGCCCA GACTGCTCAG 
GCCCCTGGTG TCACTCCACC TCCTCTTGCA ATGTCCTCCT CTCCAGCGGG ACCCCCGGCG 
CAAAAATCCC CATCGGAGTC CCGAATCCAC TCCCCTGATG AAGGCGTCCC GGCCGATGCC 
GCCCCAGAAG CGCCCGTCCC CGAAGTCCCT CAAGGCAAGA GACCGAGCCC CAGAGCGCTC 
CCTATGAGCC AGTCCACCGA GGTGGTCTAC GTGGACAAGC CGCTGCCCAA GGAATTCCCG 
GAATACCTCG TTCCATACTG GGAGAACACG TGCACGTCGT ATGTGACAAA TATCAAAGCG 
GCGATGCAGA ACCTCCGCGC CGAGCGGGAC CTGATCATCC AGCATCTCTA TGACATCAGG 
CAGGAGTTCA AGCACTACTT GATGAGACCG GACCTGAAGC AGGAGTTTGT GACCCAGTGG 
CAGCAGGACT ACAACAGCAT CCCTGAGGAC ATGAGGGCTG ACGAAGACAC CAAGGCAGAG 
CTGCACCAGC GCCTCAATGA CCTGAGCGAG CGGCTGTGGG ACATCAGTGA GCGGCGCAAG 
GAGGAGGCGC TGCAGGAGCG GACCGTGCTC ATGGGCGACG GCTGGCTGGA GGACCACACG 
GCCATGCTCA TCAACCACTT CTCCACCCTC ATGCAGGTGG AGGTGGACAG GTTCCAGGAC 
ACGGTGCATC TGCTGCGGGA CTATTACAGG GGCATGTCCG GGCAGAACCT CCCGGAAACG 
CGGTTCGCGC ACGTCCCCAT GGTGGACGTC GCCGAAGACG CTCGGGACGA GGACCCCACT 
GGCTCTGTGC AGCCTGAGCA GCAGGACACG AGCGCTGGAA CAAGCGAAGA AGAGGAGAGC 
AAGACTAAAA TAATCCCTCT GATCCCACGG AGACCTCGGT CGGGGGAAGC CGCGCTGATA 
AAGCCGAACG CCTCGACGGA CCCGCACCCG GACGAGCAGC TTTTGCATGA CATTTGGCAG 
ACGGCCGTGA CCGCTGTTAA TAACCTGGTG TCGCAGGAGA TGCCCCAGCT GGAGCCCGAG 
GAGGGTAAGG AGGCCCAGTA CGTGGCGGAG CAGGGGCCGT CTCAGCCCGC GGCCCAGAGC 
GCCAAGGACA ACGAGAAGCC CGGAAACACA AAAGGAGACA ACGCGGTGAA AGTACGCCTG 
GAGCTTGTCC GAGCGCGAGC GCTGCGCGTA TTGAGGAGCC TCCACTGCCA AGCTGACCAA 
GCTTATAGTG CCATGGAGGA GTGGCTTCAC ACCCGCTTTC AAGCAGAGAT GAACAGCATC 
AACCAGTTGG TGGAGGTGTC GCGTCACCAC ATCGAAGCGG CCGCCCAGAT CCAGCACGAG 
CTCGTGCTGG AGGGTCCCGA CTTCTTCATT GATGGGGATA CGCGCGTCGT ACCCGCTTCG 
ACGCCGCCGC CGTGCCCGCC CCCCTGGGAG CTCCCGGTTG ACAATGCCTT CACCATCCGC 
CAGCTCGAGG CATTGTACGC GCAGCTTCGC AAAGTTGCCC CGGCAGGTCG GCTGTGCGCT 
AAAGAGCTCT CGGAGATCCT GGAGGATCTC GCCTCCCTGG ACATGGGCAG CGATGCCTTG 
CCCGAACTCT GGAGCAATCT GTCCGCGTCT CAGATCCAGG ACATGGTGTC ACTCGTGAGT 
CAGGATTCCG AGGTGCTGGA CTGGCGACGC TTTCTGCTGA GCGCAGCGCT GCCGTGGCCC 
CTTCCCTCGC AGTCCCAGCT GCTGGAGGTT CTGGACCGAT TCCGAGCGAC CGACTCCCAG 
GGAACGGGCT TCGTCACCGA GGAGCTATAC CGACAGACGG AGCTCTGGTT CTCCGGGGAG 
AGCGGCGCGT CTCCTCCGGC CGACTCTTCC GAGCCCTCGC CGTTCGACCG CTTGTCGAAC 
TTGAAGAAGT TTTTCTTCGC TCTTTTTGCG GACCTCCGCT CCCCGGAACC CCGGCTCCAC 
TACGAGACCA TGCTGCTGTA CTTCGCCGCG CACCCGGACC GCGTCCGGGG TTGGGTTCGA 
GCCCTTGGCG TGGCAACGGG CCGGCTCATC TACCACCGGC CTCGCAGCGC CGTGCTCCTG 
AAGTCTGTGC CGCACGTGGG GGACGAGGAG TCGTATTACG AGCGGACGGA CGGCGAAGCG 
GAGCCCGGCG GGACAGAAGA AGGGAAGGGC GTGTCCATCG CTGCCCTCCT TAAAGTGGTC 
TGCCATGGGG GGACCAGGAC ATCCAGCTAC AACCGCTTTC ACCCCAACTG GAGGACTTGG 
GAAGAGTATG AGGAGGATTT CAGGCAGCTC TACGCAGAAC TTGGATTTGG ACCAGAAGAG 
GAGGTTCCCT TTGCCACGCT GTCCCAGCAT CCTCTGATAC AGAACCTGAT GGAAGATGCC 
GTCCAGTACC GGCTCACTGA CATCCATCAA CTTGTTTTGG CCCAGGCTAT AGAAGGAGAG 
TCGCCGTGCT TCCCGGTGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029115334.1
CDS	101..5224
Translation	MSDILCRWLNSELRLSKPVEPKTLCADLANGYLIGEVLHKYDLQPDFHRFSRSSTANAKLNNYTRLEPTMQLLGVHFDLCFAQALMQEHPGAATRLLYQLYFLLQKKKKAQLSGVAMETLQSTATAHLHRIERGIYSERLRTVVKRDVDNKLQKISQKYERKGQEMLRRSTMAQYEKELELQKVQEERRMKDIEKLRLARKRRNEMMVKIQKATVQIPRPPRKQVLRTLEHQHHKQQEAQDFQRDIEDFERNMKKQLPASALALSTPFTFSVKTDAALRPEQLAQANDEYIQRIRQRLEEDAAARQQREKRRSRILVLQQEAQEEQEELNRDERLLACLTRQSQQERRLAVQLMQVRQQKEFLRQNRIFREKQYQEQRLREFQEALDRKAALAQQTQLEHEQETQRETELHRQITAQRAQARYRSHFDTCREVLDQILNLVTKVGEYRLLMDNLIPVKLMREWKELLLLGLPLYGESASPEPSPQELEKLEVLNNQDYDDYTSMTGEWAWPEEGDAKQPPPNNNILGHVVHRLNVIVDPPKPETPPPSFPSFTLKASVLGKVFAGKSGCLARMAQVHGVHVLSTSILIQEALEAFLAGEKVPQAPPKEPQYPEGSVCLRRTAKKKDVAEKEKEKSSRPDSGHVLQSPQQENAKRTASKSEVLTSPPQLSARAQHGAALDEVLGKGQAIPDQLLVDIVLEAIRRVPPESGWILDGFPMDLDQAKLLEKALSGIDPEKVERKKNNKKINLVVDPHAKKEAPPPLPALDVVLLMDLSDDLVLDRAAKKANEMEGKLVQIQTLDEMCALAEAGSPPEPCPSTQTPDLRDKSLEMAQVQHRIAAFHDTWPKLEWWYSNRQKILVKVNADEEPDAVFKEVESIVYKIMAPPADKGVDATPTAQTAQAPGVTPPPLAMSSSPAGPPAQKSPSESRIHSPDEGVPADAAPEAPVPEVPQGKRPSPRALPMSQSTEVVYVDKPLPKEFPEYLVPYWENTCTSYVTNIKAAMQNLRAERDLIIQHLYDIRQEFKHYLMRPDLKQEFVTQWQQDYNSIPEDMRADEDTKAELHQRLNDLSERLWDISERRKEEALQERTVLMGDGWLEDHTAMLINHFSTLMQVEVDRFQDTVHLLRDYYRGMSGQNLPETRFAHVPMVDVAEDARDEDPTGSVQPEQQDTSAGTSEEEESKTKIIPLIPRRPRSGEAALIKPNASTDPHPDEQLLHDIWQTAVTAVNNLVSQEMPQLEPEEGKEAQYVAEQGPSQPAAQSAKDNEKPGNTKGDNAVKVRLELVRARALRVLRSLHCQADQAYSAMEEWLHTRFQAEMNSINQLVEVSRHHIEAAAQIQHELVLEGPDFFIDGDTRVVPASTPPPCPPPWELPVDNAFTIRQLEALYAQLRKVAPAGRLCAKELSEILEDLASLDMGSDALPELWSNLSASQIQDMVSLVSQDSEVLDWRRFLLSAALPWPLPSQSQLLEVLDRFRATDSQGTGFVTEELYRQTELWFSGESGASPPADSSEPSPFDRLSNLKKFFFALFADLRSPEPRLHYETMLLYFAAHPDRVRGWVRALGVATGRLIYHRPRSAVLLKSVPHVGDEESYYERTDGEAEPGGTEEGKGVSIAALLKVVCHGGTRTSSYNRFHPNWRTWEEYEEDFRQLYAELGFGPEEEVPFATLSQHPLIQNLMEDAVQYRLTDIHQLVLAQAIEGESPCFPVP

Target ORF information:

RefSeq Version	XM_029259501.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus sperm flagellar 2 (spef2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029259501.1

ORF Insert Sequence:

ATGTCCGATA TTTTATGCCG GTGGCTCAAC TCGGAACTGC GGCTGTCAAA ACCTGTCGAG 
CCGAAGACGC TGTGTGCCGA CCTGGCCAAC GGCTACCTGA TCGGAGAGGT TCTGCACAAA 
TACGACCTCC AGCCCGACTT CCACCGCTTC TCCAGGAGCA GCACAGCAAA TGCCAAGCTG 
AACAACTACA CGCGCCTGGA GCCCACCATG CAGCTGCTGG GTGTGCACTT TGACCTGTGC 
TTCGCACAAG CGCTGATGCA GGAGCACCCG GGCGCCGCCA CACGCCTCCT CTACCAGCTC 
TACTTCCTGC TGCAGAAGAA GAAGAAGGCG CAGCTGTCAG GAGTCGCCAT GGAGACGCTG 
CAGTCCACCG CCACGGCCCA CCTGCATCGC ATAGAGAGAG GCATTTACAG TGAGCGCCTC 
CGGACTGTGG TCAAGCGCGA CGTAGACAAT AAACTGCAGA AGATCTCCCA AAAGTACGAG 
CGCAAGGGGC AAGAGATGCT CCGGAGGTCC ACCATGGCAC AGTACGAGAA GGAGCTGGAG 
CTGCAGAAGG TGCAGGAGGA GAGGCGAATG AAGGACATTG AAAAGCTCCG TCTGGCCCGG 
AAAAGGCGCA ACGAGATGAT GGTTAAGATC CAAAAAGCCA CTGTCCAGAT CCCCAGGCCT 
CCCAGAAAGC AAGTCCTGAG GACCCTGGAG CACCAGCATC ACAAACAGCA GGAAGCTCAG 
GATTTTCAGA GGGACATTGA GGACTTTGAG AGGAACATGA AAAAGCAACT TCCTGCCAGC 
GCTTTGGCTC TGAGCACGCC CTTCACGTTT TCCGTCAAGA CGGATGCCGC CCTGCGGCCC 
GAGCAGCTGG CCCAGGCCAA CGACGAGTAC ATCCAGAGGA TCCGACAGCG GCTGGAGGAG 
GACGCCGCTG CCCGGCAGCA ACGGGAGAAA CGCAGAAGCC GCATCCTGGT GTTGCAGCAG 
GAGGCTCAAG AGGAGCAGGA GGAGTTGAAC CGCGATGAAC GGCTGCTGGC GTGTTTGACC 
CGTCAGTCAC AGCAGGAGAG GCGACTCGCC GTGCAGCTCA TGCAGGTGCG GCAGCAGAAG 
GAGTTCCTGA GACAGAACCG GATCTTCCGG GAGAAACAGT ACCAGGAGCA ACGCCTGCGG 
GAGTTCCAGG AGGCCCTGGA CCGGAAGGCG GCTCTGGCCC AGCAGACCCA ATTGGAGCAC 
GAGCAGGAGA CCCAGAGGGA GACGGAGCTG CACCGGCAGA TCACGGCCCA GCGAGCGCAA 
GCTCGGTACC GCAGCCACTT CGATACCTGC AGAGAGGTCC TGGACCAGAT ACTGAACCTG 
GTCACCAAGG TTGGGGAGTA CCGGCTCCTC ATGGACAACC TGATTCCAGT CAAGCTGATG 
AGGGAGTGGA AGGAGCTGCT GCTGCTCGGG CTGCCGCTCT ACGGTGAGAG CGCTTCCCCA 
GAGCCGAGCC CACAGGAGCT CGAGAAGCTG GAAGTCCTCA ACAACCAGGA CTATGATGAC 
TACACGAGTA TGACCGGTGA GTGGGCCTGG CCTGAGGAGG GGGATGCGAA GCAGCCCCCG 
CCCAACAACA ACATCCTGGG TCACGTGGTG CACCGGCTCA ACGTGATCGT GGACCCCCCT 
AAGCCGGAGA CCCCCCCGCC CTCGTTCCCC AGTTTCACGC TGAAGGCCTC CGTCCTGGGA 
AAGGTGTTTG CAGGGAAAAG CGGCTGTCTG GCAAGGATGG CGCAAGTCCA CGGCGTCCAC 
GTCCTGTCGA CCAGCATTCT GATCCAGGAG GCGCTGGAAG CGTTCCTGGC CGGAGAGAAG 
GTGCCGCAGG CGCCCCCGAA GGAGCCGCAG TATCCCGAGG GATCCGTCTG TCTCAGGAGG 
ACTGCGAAGA AGAAGGATGT TGCCGAGAAG GAGAAGGAGA AATCTTCACG CCCCGACTCA 
GGCCATGTCC TCCAAAGCCC CCAGCAGGAA AATGCCAAGA GGACAGCTTC CAAGTCTGAG 
GTACTGACCT CACCTCCCCA GCTGTCGGCG AGGGCTCAGC ACGGAGCCGC CCTGGACGAG 
GTCCTCGGCA AGGGGCAGGC CATCCCAGAC CAGCTCCTCG TGGACATTGT TCTGGAAGCC 
ATCAGGCGGG TCCCTCCAGA GTCGGGCTGG ATCCTCGATG GGTTCCCCAT GGACCTGGAT 
CAGGCCAAGC TCCTGGAGAA GGCTCTCAGT GGGATTGACC CAGAGAAGGT GGAAAGGAAG 
AAGAATAACA AGAAGATCAA CCTTGTCGTG GACCCCCATG CGAAGAAAGA GGCCCCACCC 
CCTCTACCTG CCCTGGACGT CGTTCTGCTT ATGGACTTGT CCGATGACCT CGTTCTGGAC 
CGTGCTGCAA AGAAAGCCAA TGAGATGGAA GGCAAGCTGG TGCAAATTCA GACTCTGGAC 
GAGATGTGCG CCTTGGCGGA GGCGGGATCT CCACCAGAGC CGTGCCCCTC GACGCAAACT 
CCTGACCTTC GGGACAAGAG CCTGGAGATG GCGCAGGTCC AGCACAGGAT CGCAGCCTTC 
CACGACACCT GGCCCAAGCT GGAGTGGTGG TACTCCAACA GGCAGAAGAT CCTGGTGAAG 
GTGAACGCGG ACGAAGAGCC CGACGCCGTC TTCAAGGAAG TGGAATCCAT CGTGTACAAG 
ATCATGGCGC CGCCGGCAGA CAAGGGTGTA GACGCGACGC CTACAGCCCA GACTGCTCAG 
GCCCCTGGTG TCACTCCACC TCCTCTTGCA ATGTCCTCCT CTCCAGCGGG ACCCCCGGCG 
CAAAAATCCC CATCGGAGTC CCGAATCCAC TCCCCTGATG AAGGCGTCCC GGCCGATGCC 
GCCCCAGAAG CGCCCGTCCC CGAAGTCCCT CAAGGCAAGA GACCGAGCCC CAGAGCGCTC 
CCTATGAGCC AGTCCACCGA GGTGGTCTAC GTGGACAAGC CGCTGCCCAA GGAATTCCCG 
GAATACCTCG TTCCATACTG GGAGAACACG TGCACGTCGT ATGTGACAAA TATCAAAGCG 
GCGATGCAGA ACCTCCGCGC CGAGCGGGAC CTGATCATCC AGCATCTCTA TGACATCAGG 
CAGGAGTTCA AGCACTACTT GATGAGACCG GACCTGAAGC AGGAGTTTGT GACCCAGTGG 
CAGCAGGACT ACAACAGCAT CCCTGAGGAC ATGAGGGCTG ACGAAGACAC CAAGGCAGAG 
CTGCACCAGC GCCTCAATGA CCTGAGCGAG CGGCTGTGGG ACATCAGTGA GCGGCGCAAG 
GAGGAGGCGC TGCAGGAGCG GACCGTGCTC ATGGGCGACG GCTGGCTGGA GGACCACACG 
GCCATGCTCA TCAACCACTT CTCCACCCTC ATGCAGGTGG AGGTGGACAG GTTCCAGGAC 
ACGGTGCATC TGCTGCGGGA CTATTACAGG GGCATGTCCG GGCAGAACCT CCCGGAAACG 
CGGTTCGCGC ACGTCCCCAT GGTGGACGTC GCCGAAGACG CTCGGGACGA GGACCCCACT 
GGCTCTGTGC AGCCTGAGCA GCAGGACACG AGCGCTGGAA CAAGCGAAGA AGAGGAGAGC 
AAGACTAAAA TAATCCCTCT GATCCCACGG AGACCTCGGT CGGGGGAAGC CGCGCTGATA 
AAGCCGAACG CCTCGACGGA CCCGCACCCG GACGAGCAGC TTTTGCATGA CATTTGGCAG 
ACGGCCGTGA CCGCTGTTAA TAACCTGGTG TCGCAGGAGA TGCCCCAGCT GGAGCCCGAG 
GAGGGTAAGG AGGCCCAGTA CGTGGCGGAG CAGGGGCCGT CTCAGCCCGC GGCCCAGAGC 
GCCAAGGACA ACGAGAAGCC CGGAAACACA AAAGGAGACA ACGCGGTGAA AGTACGCCTG 
GAGCTTGTCC GAGCGCGAGC GCTGCGCGTA TTGAGGAGCC TCCACTGCCA AGCTGACCAA 
GCTTATAGTG CCATGGAGGA GTGGCTTCAC ACCCGCTTTC AAGCAGAGAT GAACAGCATC 
AACCAGTTGG TGGAGGTGTC GCGTCACCAC ATCGAAGCGG CCGCCCAGAT CCAGCACGAG 
CTCGTGCTGG AGGGTCCCGA CTTCTTCATT GATGGGGATA CGCGCGTCGT ACCCGCTTCG 
ACGCCGCCGC CGTGCCCGCC CCCCTGGGAG CTCCCGGTTG ACAATGCCTT CACCATCCGC 
CAGCTCGAGG CATTGTACGC GCAGCTTCGC AAAGTTGCCC CGGCAGGTCG GCTGTGCGCT 
AAAGAGCTCT CGGAGATCCT GGAGGATCTC GCCTCCCTGG ACATGGGCAG CGATGCCTTG 
CCCGAACTCT GGAGCAATCT GTCCGCGTCT CAGATCCAGG ACATGGTGTC ACTCGTGAGT 
CAGGATTCCG AGGTGCTGGA CTGGCGACGC TTTCTGCTGA GCGCAGCGCT GCCGTGGCCC 
CTTCCCTCGC AGTCCCAGCT GCTGGAGGTT CTGGACCGAT TCCGAGCGAC CGACTCCCAG 
GGAACGGGCT TCGTCACCGA GGAGCTATAC CGACAGACGG AGCTCTGGTT CTCCGGGGAG 
AGCGGCGCGT CTCCTCCGGC CGACTCTTCC GAGCCCTCGC CGTTCGACCG CTTGTCGAAC 
TTGAAGAAGT TTTTCTTCGC TCTTTTTGCG GACCTCCGCT CCCCGGAACC CCGGCTCCAC 
TACGAGACCA TGCTGCTGTA CTTCGCCGCG CACCCGGACC GCGTCCGGGG TTGGGTTCGA 
GCCCTTGGCG TGGCAACGGG CCGGCTCATC TACCACCGGC CTCGCAGCGC CGTGCTCCTG 
AAGTCTGTGC CGCACGTGGG GGACGAGGAG TCGTATTACG AGCGGACGGA CGGCGAAGCG 
GAGCCCGGCG GGACAGAAGA AGGGAAGGGC GTGTCCATCG CTGCCCTCCT TAAAGTGGTC 
TGCCATGGGG GGACCAGGAC ATCCAGCTAC AACCGCTTTC ACCCCAACTG GAGGACTTGG 
GAAGAGTATG AGGAGGATTT CAGGCAGCTC TACGCAGAAC TTGGATTTGG ACCAGAAGAG 
GAGGTTCCCT TTGCCACGCT GTCCCAGCAT CCTCTGATAC AGAACCTGAT GGAAGATGCC 
GTCCAGTACC GGCTCACTGA CATCCATCAA CTTGTTTTGG CCCAGGCTAT AGAAGGAGAG 
TCGCCGTGCT TCCCGGTGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

spef2 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム