LOC108919738 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108919738
Entrez Gene ID	108919738
Full Name	NACHT, LRR and PYD domains-containing protein 4E-like
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018727975.1	XP_018583491.1	NACHT, LRR and PYD domains-containing protein 4E-like
XM_029251964.1	XP_029107797.1	uncharacterized protein LOC108919738

Pathways from BioSystems
Gene Ontology

The following LOC108919738 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108919738 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc139267	XM_029251964.1 Latest version!	Scleropages formosus uncharacterized LOC108919738 (LOC108919738), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSc67606	XM_018727975.1 Latest version!	Scleropages formosus NACHT, LRR and PYD domains-containing protein 4E-like (LOC108919738), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108919738 ( XM_029251964.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107797.1 Scleropages formosus uncharacterized LOC108919738 (LOC108919738), mRNA.

Price & Availability↑

CloneID	OSc139267
Clone ID Related Accession (Same CDS sequence)	XM_029251964.1
Accession Version	XM_029251964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	uncharacterized protein LOC108919738
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTC ACAAAAGCGT TCAGGAGCAG AGACCAGGAT CTCCAGCAGC CATCTGTATG 
TCAATGAGGA ATGACCGGTC CATGTCTGAA CCCATTAGGT CCAGTGGTGG AACTACCATG 
GCACAGGACC AGAGACCATG GTCTCCAGCA CCCAGCTGTA TGTCGATGAA GAGTGACCGG 
TCCATGCCTG AACCCATTAG GTTCAGTGGT GGAACTGCAA TGGCACAGGA CCAGAGACCA 
TGGTCTCCAG CACCCAGCTG TATGTCGATG AAGAGTGACC GGTCCATGCC TGAACCCATT 
AGGTTCAGTG GTGGAACTGC CATGGCACAG GACCAGAGAC CATGGTCTCC AGCAGTCATC 
TATATGTCAA TGAGGAATGA CCAGTCCATG TCTGAACCCA TTAGGTTCAG TGGTGGAACT 
GCCATGGCAC AGGACCAGAG ACCATGGTCT CCAGCAGTCA TCTATATGTC AATGAGGAAT 
GACCAGTCCA TGTCTGAACC CATTAGGTTC AGTGGTGGAA CTGCCATGGC ACAGGACCAG 
AGACCAGGAT CTCCAGCAGC CATCTGTATG TCAATGAGGA ATGACCGGTC CATGCCTGAA 
CCCATTAGGT TCAGTGGTGG AACTGCCATG GCACAGGACC AGAGACCATG GTCTCCAGCA 
CCCAGCTGTA TGTCGATGAA GAGTGACCGG TCCATGCCTG AACCCATTAG GTTCAGTGGT 
GGAACTGCCA TGGCACAGGA CCAGAGACCA TGGTCTCCAG GACCCAGCTG TATGTCGATG 
AAGAGTGACC GGTCCATGCC TGAACCCATT AGGTTCAGTG GTGGAACTGC CATGGCACAG 
GACCAGAGAC CATGGTCTCC AGCACCCAGC TGTATGTCGA TGAAGAGTGA CCGGTCCATG 
CCTGAACCCA TTAGGTTCAG TGGTGGAACT GCCATGGCAC AGGACCAGAG ACCATGGTCT 
CCAGGACCCA GCTGTATGTC GATGAAGAGT GACCGGTCCA TGCCTGAACC CATTAGGTTC 
AGTGGTGGAA CTGCCATGGC ACAGGACCAG AGACCAGGGT CTCCAGCACC CAGCTGCATG 
TTGATGAAGA GTGACCGGTC CATGCCTGAA CCCATTAGGT TCAGTGGTGG AACTGCCATG 
GCACAGGACC AGAGACTATG GTCTCCAGGA CCCAGCTGTA TGTCGATGAA GAGTGACCGG 
TCCATGCCTG AACCCATTAG GTTCAGTGGT GGAACTGCCA TGGCACAGGA CCAGAGACCA 
TGGTCTCCAG CACCCAGCTG TATGTCGATG AAGAGTGACC GGTCCATGCC TGAACCCATT 
AGGTTCAGTG GTGGAACTGC CATGGCACAG GACCAGAGAC CATGGTCTCC AGCAGTCATC 
TATATGTCAA TGAGGAATGA CCAGTCCATG TCTGAACCCA TTAGGTTCAG TGGTGGAACT 
GCCATGGCAC AGGACCAGAG ACCAGGATCT CTAGCAGCCA TCTGTATGTC AATGAGGAAT 
GACTGGTCCA TGCCTGAACC CATTAGGCTC AAAAATGGAG CTGAACAAGA TGCTTCAGTC 
ATTTACAGTA GTGGACACAC TAATGCAAGG GGGAAGAGAA AAGCGCCCCC AGTGGCCAGC 
CGTGTGTCTG TGAAGAGACT CTGTGTGAGG GGAAGATCCA ATGAGTTTAC TGGTGTGACG 
TTCCCTATTG GTCAAGGCTG TTTATACACT GGATACACCA GTACAAGTGA GAAACAACAG 
GAAATGGAGC CTAGTGATGA CAAGGAGGAG ATGCTGGACC AGCAGTCCTA CATTGTAGTA 
CTGCAGAGAC ACCAACTGAT GGAGCCCAGT GAAAATCTGG ACCCAGAGTC TTTCCAGAGG 
TCAAAGTCAA ATGCCTCATT GTTGAGAGAA ATACACAGAG ACTATGATGT GAGGAGAGTC 
CAGATGAAAC ACAAGACCAA ACTAGAATCT ACATTTAAAC ACATTTTTGA AGGGATTGCA 
AAGCAGGGAA ACCCAACCCT CCTGAACAGA ATCTACACTG AGCTATACAT CACAGAGGGA 
GAGAGAGAAG GAGTCAATGA TGAACATGAG GTGTGGCAGA TCGAGAGTGC TTACAGGACA 
CACAGTTCAC TAGACACTCC TATCAACTGC AACGACATTT TCAAGTGCCT ACCTGGTCAA 
GAGAGAACCA TCAGAACTGT GCTGACCAAG GGCATTGCTG GCATTGGAAA AACAGTTTCT 
GTGCAGAAGT TCATCCTTGA CTGGGCTGAA GGAAAAGCCA ATCAGGACAT TGATTTCATT 
TTTGTTCTTC CTTTTCGGGA ACTCAATTTA ATTAAAGATG AGCAGTACAG TCTTCTTGAA 
CTTCTTCATG AGTTCTATCC AGAAATAGAA AATATCAAAG ACATCAGATT TAATAAATAT 
AAAGTTATGC TGATCTTTGA TGGTCTGGAT GAAAGCAGAC TTCCGTTGAA TTTTCATCAC 
ATGAGGTTGT CTGATGTTAA GGAGGCCACA TCAGTCGATG TGTTGTTGAC CAACCTCATT 
AAGAGGATTC TTCTTCCTTC TGCTCTCCTG TGGATAACAA CCAGGCCAGC AGCAGCCAGT 
CAGATCCCTC CTGAGTATGT TGACCAGGTG ACAGAGATTC GGGGGTTTAA TGACCCACAA 
AAAGAGGAGT ATTTTCATAA AAGATTCAGT GATGAGGATC TGGCCAACAG AATTATATCT 
CACATACAGT CATCAAGGAG CCTCTACATC ATGTGTCACA TACCAGTTTT CTGTTGGATT 
TCAGCAACTG TTCTTGAGAA AATGCTAGGT AATGATGGTG GTGAAGAAAT ACCTACAACT 
CTGACACAAA TGTATACACA CTTCCTGCTC ATTCAGACCA ACATAAAGAA CAAGAAGTAT 
CATGGAACCA ATGAGACCGA CTCCAGAAAC GTGTCACAGT CAGACAGAGA TCTTATACTT 
AAGTTGGCAG AGTTAGCTTT CCACCAGCTG GAAAAGGGAA ATATCATATT TTATGAAACG 
GACTTGAAAG ACTGTGACAT TGATGTCAGT GAAGTATCAG TGTATTCTGG GGTGTGCACC 
CAAATCTTCA AGGAGGAATC AGTGATGTAT AAGAAGAAGG TGTACTGCTT TGTGCATTTG 
AGCATTCAGG AGTTTCTTGC TGCTCTGTAT GTCATCCACT GTCATGCCAG CAATAAAATG 
GATTCCCTCA AACTTTTTTC TGAAAAAAAG TCAAGAGTCT CACATCTCGA AATGCTTTTG 
AATGAGTTGC ACAAACTCGC AGTTAACAAG GCCTTGGAGA GTAAGAATGG ACATCTGGAC 
CTCTTCCTTC GCTTCCTTCT TGGCCTCTCA ATGGACTCCA ACAAGAGTCT CTTACAAGGG 
TTGTTTACAC AGACTGAGAG CAGCTCAGAG AGCATCAAGA AAACAGTTAA ATACATTAAA 
GTACTGAGGA CGAAGTATCC TTCACCTGAG AGGTATATGA ATCTTTTCCT CTGTTTGGTT 
GAATTGAATG ACTTTTCCCT TCTGAAGAAA GTCCAGAAGT ACCGGGACAG TTCTTCAGGG 
GAGAAACTCA CTCCTGCAGA GTGTTCCTGT CTGGCCTATG TGCTCCTCAT GTCGAATGAG 
GTATTGGATG TGTTTGATAT CTCAACATTC AACACGTCAC CAGAGGGTCG TATCAGACTC 
ATCCCAGCTG TGAAGTGCTG CAGAAAGGCT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107797.1
CDS	99..3794
Translation	MDPHKSVQEQRPGSPAAICMSMRNDRSMSEPIRSSGGTTMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAVIYMSMRNDQSMSEPIRFSGGTAMAQDQRPWSPAVIYMSMRNDQSMSEPIRFSGGTAMAQDQRPGSPAAICMSMRNDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPGPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPGPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPGSPAPSCMLMKSDRSMPEPIRFSGGTAMAQDQRLWSPGPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAVIYMSMRNDQSMSEPIRFSGGTAMAQDQRPGSLAAICMSMRNDWSMPEPIRLKNGAEQDASVIYSSGHTNARGKRKAPPVASRVSVKRLCVRGRSNEFTGVTFPIGQGCLYTGYTSTSEKQQEMEPSDDKEEMLDQQSYIVVLQRHQLMEPSENLDPESFQRSKSNASLLREIHRDYDVRRVQMKHKTKLESTFKHIFEGIAKQGNPTLLNRIYTELYITEGEREGVNDEHEVWQIESAYRTHSSLDTPINCNDIFKCLPGQERTIRTVLTKGIAGIGKTVSVQKFILDWAEGKANQDIDFIFVLPFRELNLIKDEQYSLLELLHEFYPEIENIKDIRFNKYKVMLIFDGLDESRLPLNFHHMRLSDVKEATSVDVLLTNLIKRILLPSALLWITTRPAAASQIPPEYVDQVTEIRGFNDPQKEEYFHKRFSDEDLANRIISHIQSSRSLYIMCHIPVFCWISATVLEKMLGNDGGEEIPTTLTQMYTHFLLIQTNIKNKKYHGTNETDSRNVSQSDRDLILKLAELAFHQLEKGNIIFYETDLKDCDIDVSEVSVYSGVCTQIFKEESVMYKKKVYCFVHLSIQEFLAALYVIHCHASNKMDSLKLFSEKKSRVSHLEMLLNELHKLAVNKALESKNGHLDLFLRFLLGLSMDSNKSLLQGLFTQTESSSESIKKTVKYIKVLRTKYPSPERYMNLFLCLVELNDFSLLKKVQKYRDSSSGEKLTPAECSCLAYVLLMSNEVLDVFDISTFNTSPEGRIRLIPAVKCCRKAV

Target ORF information:

RefSeq Version	XM_029251964.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus uncharacterized LOC108919738 (LOC108919738), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029251964.1

ORF Insert Sequence:

ATGGATCCTC ACAAAAGCGT TCAGGAGCAG AGACCAGGAT CTCCAGCAGC CATCTGTATG 
TCAATGAGGA ATGACCGGTC CATGTCTGAA CCCATTAGGT CCAGTGGTGG AACTACCATG 
GCACAGGACC AGAGACCATG GTCTCCAGCA CCCAGCTGTA TGTCGATGAA GAGTGACCGG 
TCCATGCCTG AACCCATTAG GTTCAGTGGT GGAACTGCAA TGGCACAGGA CCAGAGACCA 
TGGTCTCCAG CACCCAGCTG TATGTCGATG AAGAGTGACC GGTCCATGCC TGAACCCATT 
AGGTTCAGTG GTGGAACTGC CATGGCACAG GACCAGAGAC CATGGTCTCC AGCAGTCATC 
TATATGTCAA TGAGGAATGA CCAGTCCATG TCTGAACCCA TTAGGTTCAG TGGTGGAACT 
GCCATGGCAC AGGACCAGAG ACCATGGTCT CCAGCAGTCA TCTATATGTC AATGAGGAAT 
GACCAGTCCA TGTCTGAACC CATTAGGTTC AGTGGTGGAA CTGCCATGGC ACAGGACCAG 
AGACCAGGAT CTCCAGCAGC CATCTGTATG TCAATGAGGA ATGACCGGTC CATGCCTGAA 
CCCATTAGGT TCAGTGGTGG AACTGCCATG GCACAGGACC AGAGACCATG GTCTCCAGCA 
CCCAGCTGTA TGTCGATGAA GAGTGACCGG TCCATGCCTG AACCCATTAG GTTCAGTGGT 
GGAACTGCCA TGGCACAGGA CCAGAGACCA TGGTCTCCAG GACCCAGCTG TATGTCGATG 
AAGAGTGACC GGTCCATGCC TGAACCCATT AGGTTCAGTG GTGGAACTGC CATGGCACAG 
GACCAGAGAC CATGGTCTCC AGCACCCAGC TGTATGTCGA TGAAGAGTGA CCGGTCCATG 
CCTGAACCCA TTAGGTTCAG TGGTGGAACT GCCATGGCAC AGGACCAGAG ACCATGGTCT 
CCAGGACCCA GCTGTATGTC GATGAAGAGT GACCGGTCCA TGCCTGAACC CATTAGGTTC 
AGTGGTGGAA CTGCCATGGC ACAGGACCAG AGACCAGGGT CTCCAGCACC CAGCTGCATG 
TTGATGAAGA GTGACCGGTC CATGCCTGAA CCCATTAGGT TCAGTGGTGG AACTGCCATG 
GCACAGGACC AGAGACTATG GTCTCCAGGA CCCAGCTGTA TGTCGATGAA GAGTGACCGG 
TCCATGCCTG AACCCATTAG GTTCAGTGGT GGAACTGCCA TGGCACAGGA CCAGAGACCA 
TGGTCTCCAG CACCCAGCTG TATGTCGATG AAGAGTGACC GGTCCATGCC TGAACCCATT 
AGGTTCAGTG GTGGAACTGC CATGGCACAG GACCAGAGAC CATGGTCTCC AGCAGTCATC 
TATATGTCAA TGAGGAATGA CCAGTCCATG TCTGAACCCA TTAGGTTCAG TGGTGGAACT 
GCCATGGCAC AGGACCAGAG ACCAGGATCT CTAGCAGCCA TCTGTATGTC AATGAGGAAT 
GACTGGTCCA TGCCTGAACC CATTAGGCTC AAAAATGGAG CTGAACAAGA TGCTTCAGTC 
ATTTACAGTA GTGGACACAC TAATGCAAGG GGGAAGAGAA AAGCGCCCCC AGTGGCCAGC 
CGTGTGTCTG TGAAGAGACT CTGTGTGAGG GGAAGATCCA ATGAGTTTAC TGGTGTGACG 
TTCCCTATTG GTCAAGGCTG TTTATACACT GGATACACCA GTACAAGTGA GAAACAACAG 
GAAATGGAGC CTAGTGATGA CAAGGAGGAG ATGCTGGACC AGCAGTCCTA CATTGTAGTA 
CTGCAGAGAC ACCAACTGAT GGAGCCCAGT GAAAATCTGG ACCCAGAGTC TTTCCAGAGG 
TCAAAGTCAA ATGCCTCATT GTTGAGAGAA ATACACAGAG ACTATGATGT GAGGAGAGTC 
CAGATGAAAC ACAAGACCAA ACTAGAATCT ACATTTAAAC ACATTTTTGA AGGGATTGCA 
AAGCAGGGAA ACCCAACCCT CCTGAACAGA ATCTACACTG AGCTATACAT CACAGAGGGA 
GAGAGAGAAG GAGTCAATGA TGAACATGAG GTGTGGCAGA TCGAGAGTGC TTACAGGACA 
CACAGTTCAC TAGACACTCC TATCAACTGC AACGACATTT TCAAGTGCCT ACCTGGTCAA 
GAGAGAACCA TCAGAACTGT GCTGACCAAG GGCATTGCTG GCATTGGAAA AACAGTTTCT 
GTGCAGAAGT TCATCCTTGA CTGGGCTGAA GGAAAAGCCA ATCAGGACAT TGATTTCATT 
TTTGTTCTTC CTTTTCGGGA ACTCAATTTA ATTAAAGATG AGCAGTACAG TCTTCTTGAA 
CTTCTTCATG AGTTCTATCC AGAAATAGAA AATATCAAAG ACATCAGATT TAATAAATAT 
AAAGTTATGC TGATCTTTGA TGGTCTGGAT GAAAGCAGAC TTCCGTTGAA TTTTCATCAC 
ATGAGGTTGT CTGATGTTAA GGAGGCCACA TCAGTCGATG TGTTGTTGAC CAACCTCATT 
AAGAGGATTC TTCTTCCTTC TGCTCTCCTG TGGATAACAA CCAGGCCAGC AGCAGCCAGT 
CAGATCCCTC CTGAGTATGT TGACCAGGTG ACAGAGATTC GGGGGTTTAA TGACCCACAA 
AAAGAGGAGT ATTTTCATAA AAGATTCAGT GATGAGGATC TGGCCAACAG AATTATATCT 
CACATACAGT CATCAAGGAG CCTCTACATC ATGTGTCACA TACCAGTTTT CTGTTGGATT 
TCAGCAACTG TTCTTGAGAA AATGCTAGGT AATGATGGTG GTGAAGAAAT ACCTACAACT 
CTGACACAAA TGTATACACA CTTCCTGCTC ATTCAGACCA ACATAAAGAA CAAGAAGTAT 
CATGGAACCA ATGAGACCGA CTCCAGAAAC GTGTCACAGT CAGACAGAGA TCTTATACTT 
AAGTTGGCAG AGTTAGCTTT CCACCAGCTG GAAAAGGGAA ATATCATATT TTATGAAACG 
GACTTGAAAG ACTGTGACAT TGATGTCAGT GAAGTATCAG TGTATTCTGG GGTGTGCACC 
CAAATCTTCA AGGAGGAATC AGTGATGTAT AAGAAGAAGG TGTACTGCTT TGTGCATTTG 
AGCATTCAGG AGTTTCTTGC TGCTCTGTAT GTCATCCACT GTCATGCCAG CAATAAAATG 
GATTCCCTCA AACTTTTTTC TGAAAAAAAG TCAAGAGTCT CACATCTCGA AATGCTTTTG 
AATGAGTTGC ACAAACTCGC AGTTAACAAG GCCTTGGAGA GTAAGAATGG ACATCTGGAC 
CTCTTCCTTC GCTTCCTTCT TGGCCTCTCA ATGGACTCCA ACAAGAGTCT CTTACAAGGG 
TTGTTTACAC AGACTGAGAG CAGCTCAGAG AGCATCAAGA AAACAGTTAA ATACATTAAA 
GTACTGAGGA CGAAGTATCC TTCACCTGAG AGGTATATGA ATCTTTTCCT CTGTTTGGTT 
GAATTGAATG ACTTTTCCCT TCTGAAGAAA GTCCAGAAGT ACCGGGACAG TTCTTCAGGG 
GAGAAACTCA CTCCTGCAGA GTGTTCCTGT CTGGCCTATG TGCTCCTCAT GTCGAATGAG 
GTATTGGATG TGTTTGATAT CTCAACATTC AACACGTCAC CAGAGGGTCG TATCAGACTC 
ATCCCAGCTG TGAAGTGCTG CAGAAAGGCT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108919738 ( XM_018727975.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018583491.1 Scleropages formosus NACHT, LRR and PYD domains-containing protein 4E-like (LOC108919738), mRNA.

Price & Availability↑

CloneID	OSc67606
Clone ID Related Accession (Same CDS sequence)	XM_018727975.1
Accession Version	XM_018727975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	NACHT, LRR and PYD domains-containing protein 4E-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372350.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGATGA AGAGTGACCG GTCCATGCCT GAACCCATTA GGTCCAGTGG TGGAACTGCC 
ATGGCACAGG ACCAGAGACC AGGGTCTCCA GCACCCAGCT GCATGTTGAT GAAGAGTGAC 
CGGTCCATGC CTGAACCCAT TAGGTTCAGT GGTGGAACTG CCATGGCACA GGACCAGAGA 
CCATGGTCTC CAGCACCCAG CTGTATGTCG ATGAAGAGTG ACCGGTCCAT GCCTGAACCC 
ATTAGGTTCA GTGGTGGAAC TGCCATGGCA CAGGACCAGA GACCATGGTC TCCAGCACCC 
AGCTGTATGT CGATGAAGAG TGACCGGTCC ATGCCTGAAC CCATTAGGTT CAGTGGTGGA 
ACTGCCATGG CACAGGACCA GAGACCATGG TCTCCAGCAC CCAGCTGTAT GTCGATGAAG 
AGTGACCGGT CCATGCCTGA ACCCATTAGG TTCAGTGGTG GAACTGCCAT GGCACAGGAC 
CAGAGACCAT GGTCTCCAGC AGTCATCTAT ATGTCAATGA GGAATGACCA GTCCATGTCT 
GAACCCATTA GGTTCAGTGG TGGAACTGCC ATGGCACAGG ACCAGAGACC AGGATCTCTA 
GCAGCCATCT GTATGTCAAT GAGGAATGAC TGGTCCATGC CTGAACCCAT TAGGCTCAAA 
AATGGAGCTG AACAAGATGC TTCAGTCATT TACAGTAGTG GACACACTAA TGCAAGGGGG 
AAGAGAAAAG CGCCCCCAGT GGCCAGCCGT GTGTCTGTGA AGAGACTCTG TGTGAGGGGA 
AGATCCAATG AGTTTACTGG TGTGACGTTC CCTATTGGTC AAGGCTGTTT ATACACTGGA 
TACACCAGTA CAAGTGAGAA ACAACAGGAA ATGGAGCCTA GTGATGACAA GGAGGAGATG 
CTGGACCAGC AGTCCTACAT TGTAGTACTG CAGAGACACC AACTGATGGA GCCCAGTGAA 
AATCTGGACC CAGAGTCTTT CCAGAGGTCA AAGTCAAATG CCTCATTGTT GAGAGAAATA 
CACAGAGACT ATGATGTGAG GAGAGTCCAG ATGAAACACA AGACCAAACT AGAATCTACA 
TTTAAACACA TTTTTGAAGG GATTGCAAAG CAGGGAAACC CAACCCTCCT GAACAGAATC 
TACACTGAGC TATACATCAC AGAGGGAGAG AGAGAAGGAG TCAATGATGA ACATGAGGTG 
TGGCAGATCG AGAGTGCTTA CAGGACACAC AGTTCACTAG ACACTCCTAT CAACTGCAAC 
GACATTTTCA AGTGCCTACC TGGTCAAGAG AAAACCATCA GAACTGTGCT GACCAAGGGC 
ATTGCTGGCA TTGGAAAAAC AGTTTCTGTG CAGAAGTTCA TCCTTGACTG GGCTGAAGGA 
AAAGCCAATC AGGACATTGA TTTCATTTTT GTTCTTCCTT TTCGGGAACT CAATTTAATT 
AAAGATGAGC AGTACAGTCT TCTTGAACTT CTTCATGAGT TCTATCCAGA AATAGAAAAT 
ATCAAAGACA TCAGATTTAA TAAATATAAA GTTATGCTGA TCTTTGATGG TCTGGATGAA 
AGCAGACTTC CGTTGAATTT TCATCACACG AGGTTGTCTG ATGTTAAGGA GGCCACATCA 
GTCGATGTGT TGTTGACCAA CCTCATTAAG AGGATTCTTC TTCCTTCTGC TCTCCTGTGG 
ATAACAACCA GGCCAGCAGC AGCCAGTCAG ATCCCTCCTG AGTATGTTGA CCAGGTGACA 
GAGATTCGGG GGTTTAATGA CCCACAAAAA GAGGAGTATT TTCATAAAAG ATTCAGTGAT 
GAGGATCTGG CCAACAGAAT TATATCTCAC ATACAGTCAT CAAGGAGCCT CTACATCATG 
TGTCACATAC CAGTTTTCTG TTGGATTTCA GCAACTGTTC TTGAGAAAAT GCTAGGTAAT 
GATGGTGGTG AAGAAATACC TACAACTCTG ACACAAATGT ATACACACTT CCTGCTCATT 
CAGACCAACA TAAAGAACAA GAAGTATCAT GGAACCAATG AGACCGACTC CAGAAACGTG 
TCACAGTCAG ACAGAGATCT TATACTTAAG TTGGCAGAGT TAGCTTTCCA CCAGCTGGAA 
AAGGGAAATA TCATATTTTA TGAAACGGAC TTGAAAGACT GTGACATTGA TGTCAGTGAA 
GCATCAGTGT ATTCTGGGGT GTGCACCCAA ATCTTCAAGG AGGAATCAGT GATGTATAAG 
AAGAAGGTGT ACTGCTTTGT GCATTTGAGC GTTCAGGAGT TTCTTGCTGC TCTGTATGTC 
ATCCACTGTC ATGCCAGCAA TAAAATGGAT TCCCTCAAAC TTTTCTCTGA AAGAAAGTCA 
AGAGTCTCAC ATCTCGAAAT GCTTTTGAAT GAGTTGCACA AACTCGCAGT TAACAAGGCC 
TTGGAAAGTA AGAATGGACA TCTGGACCTC TTCCTTCGCT TCCTTCTTGG CCTCTCAATG 
GACTCCAACA AGAGTCTCTT ACAAGGGTTG TTTACACAGA CTGAGAGCAG CTCAGAGAGC 
ATCAAGAAAA CAGTTAAATA CATTAAAGTA CTGAGGACGA AGTATCCTTC ACCTGAGAGG 
TATATGAATC TTTTCCTCTG TTTGGTTGAA TTGAATGACT TTTCCCTTCT GAAGAAAGTC 
CAGAAGTACC GGGACAGTTA TTCAGGGGAG AAACTCACTC CTGCAGAGTG TTCCTGTCTG 
GCCTATGTAC TCCTCATGTC GAATGAGGTA TTGGATGTGT TTGATATCTC AACATTCAAC 
ACGTCACCAG AGGGTCGTAT CAGACTCATC CCAGCTGTGA AGTGCTGCAG AAAGGCTGTA 
TTAACTCACT GTAAACTGAC AGGAGATTCC TGTGGATCTG TGGTCTCAGC TCTCCAGTCA 
GTGAACTCAC AGCTGACAGA ACTGGATCTC AGCTACAATC ACCTGGGGGA TTCAGGAGTG 
AAGGAGCTCT GTACTGGACT GATGAGTCCT CACTGTAAAC TACACACTCT GAGGTTGTCC 
CTGTGTAGAA TCACAGAGAG AGGCTGTTCT TCTCTGGCTT CAGCTCTGGG TTCGAACCCC 
CGCTCACAGC TGAGAGAGCT GGATCTGAGC TATAATCACC CAGGGGACTC AGGAGTGAAG 
CTGCTCTCTG CTCTACCGGA GAAAGTGAAT GTGGACCATG CTGGACAATG CAGGATCAGA 
CCAGGGCTCC TCAAGTACTC CTGCCAGTTC ACACTGGACC CCCACACAGC AAACAGAAAA 
CTAGTCCTGT CTGAGGACAA GAGGACAGTG ACATGGAGGG GAGAGGAACA AAAATATCCT 
GATCATCCAG AGAGATTTGA CTGTTTGAGA CAAGTTCTGT GTGTAGAGAG TGTGTCTCAT 
CGCTCTTACT GGGAGGTTCA GTGGACTGGA AATGAGGCTG AAATAGGAGT GACTTATAAA 
GGAATCAAGA GGAAAGGAGA TGGTGATGAC TGTCGGCTTG GATACAATGG CAAGTCCTGG 
GTTCTGTGCT GCACTGATAA GAGATACTCT GTGAGACACA ATAACACAAA CACTGCCATA 
GCTGCACCCC CCTCCCCCAG AGTAGGAGTG TATGTGGACT GTGTGTCCGG CACTCTGTCC 
TTCTACAGCG TCTCCTCTGG TGAACCGACC CTCCTGTACA GCTTCACCTC CACATTCACT 
GAGCCCCTCT ATCCTGTGTT TTCGCTTTGG GGTTCAGATT CTTCAGTGTC CCTGTGTGAC 
CTGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018583491.1
CDS	1..3729
Translation	MSMKSDRSMPEPIRSSGGTAMAQDQRPGSPAPSCMLMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAPSCMSMKSDRSMPEPIRFSGGTAMAQDQRPWSPAVIYMSMRNDQSMSEPIRFSGGTAMAQDQRPGSLAAICMSMRNDWSMPEPIRLKNGAEQDASVIYSSGHTNARGKRKAPPVASRVSVKRLCVRGRSNEFTGVTFPIGQGCLYTGYTSTSEKQQEMEPSDDKEEMLDQQSYIVVLQRHQLMEPSENLDPESFQRSKSNASLLREIHRDYDVRRVQMKHKTKLESTFKHIFEGIAKQGNPTLLNRIYTELYITEGEREGVNDEHEVWQIESAYRTHSSLDTPINCNDIFKCLPGQEKTIRTVLTKGIAGIGKTVSVQKFILDWAEGKANQDIDFIFVLPFRELNLIKDEQYSLLELLHEFYPEIENIKDIRFNKYKVMLIFDGLDESRLPLNFHHTRLSDVKEATSVDVLLTNLIKRILLPSALLWITTRPAAASQIPPEYVDQVTEIRGFNDPQKEEYFHKRFSDEDLANRIISHIQSSRSLYIMCHIPVFCWISATVLEKMLGNDGGEEIPTTLTQMYTHFLLIQTNIKNKKYHGTNETDSRNVSQSDRDLILKLAELAFHQLEKGNIIFYETDLKDCDIDVSEASVYSGVCTQIFKEESVMYKKKVYCFVHLSVQEFLAALYVIHCHASNKMDSLKLFSERKSRVSHLEMLLNELHKLAVNKALESKNGHLDLFLRFLLGLSMDSNKSLLQGLFTQTESSSESIKKTVKYIKVLRTKYPSPERYMNLFLCLVELNDFSLLKKVQKYRDSYSGEKLTPAECSCLAYVLLMSNEVLDVFDISTFNTSPEGRIRLIPAVKCCRKAVLTHCKLTGDSCGSVVSALQSVNSQLTELDLSYNHLGDSGVKELCTGLMSPHCKLHTLRLSLCRITERGCSSLASALGSNPRSQLRELDLSYNHPGDSGVKLLSALPEKVNVDHAGQCRIRPGLLKYSCQFTLDPHTANRKLVLSEDKRTVTWRGEEQKYPDHPERFDCLRQVLCVESVSHRSYWEVQWTGNEAEIGVTYKGIKRKGDGDDCRLGYNGKSWVLCCTDKRYSVRHNNTNTAIAAPPSPRVGVYVDCVSGTLSFYSVSSGEPTLLYSFTSTFTEPLYPVFSLWGSDSSVSLCDLE

Target ORF information:

RefSeq Version	XM_018727975.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus NACHT, LRR and PYD domains-containing protein 4E-like (LOC108919738), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018727975.1

ORF Insert Sequence:

ATGTCGATGA AGAGTGACCG GTCCATGCCT GAACCCATTA GGTCCAGTGG TGGAACTGCC 
ATGGCACAGG ACCAGAGACC AGGGTCTCCA GCACCCAGCT GCATGTTGAT GAAGAGTGAC 
CGGTCCATGC CTGAACCCAT TAGGTTCAGT GGTGGAACTG CCATGGCACA GGACCAGAGA 
CCATGGTCTC CAGCACCCAG CTGTATGTCG ATGAAGAGTG ACCGGTCCAT GCCTGAACCC 
ATTAGGTTCA GTGGTGGAAC TGCCATGGCA CAGGACCAGA GACCATGGTC TCCAGCACCC 
AGCTGTATGT CGATGAAGAG TGACCGGTCC ATGCCTGAAC CCATTAGGTT CAGTGGTGGA 
ACTGCCATGG CACAGGACCA GAGACCATGG TCTCCAGCAC CCAGCTGTAT GTCGATGAAG 
AGTGACCGGT CCATGCCTGA ACCCATTAGG TTCAGTGGTG GAACTGCCAT GGCACAGGAC 
CAGAGACCAT GGTCTCCAGC AGTCATCTAT ATGTCAATGA GGAATGACCA GTCCATGTCT 
GAACCCATTA GGTTCAGTGG TGGAACTGCC ATGGCACAGG ACCAGAGACC AGGATCTCTA 
GCAGCCATCT GTATGTCAAT GAGGAATGAC TGGTCCATGC CTGAACCCAT TAGGCTCAAA 
AATGGAGCTG AACAAGATGC TTCAGTCATT TACAGTAGTG GACACACTAA TGCAAGGGGG 
AAGAGAAAAG CGCCCCCAGT GGCCAGCCGT GTGTCTGTGA AGAGACTCTG TGTGAGGGGA 
AGATCCAATG AGTTTACTGG TGTGACGTTC CCTATTGGTC AAGGCTGTTT ATACACTGGA 
TACACCAGTA CAAGTGAGAA ACAACAGGAA ATGGAGCCTA GTGATGACAA GGAGGAGATG 
CTGGACCAGC AGTCCTACAT TGTAGTACTG CAGAGACACC AACTGATGGA GCCCAGTGAA 
AATCTGGACC CAGAGTCTTT CCAGAGGTCA AAGTCAAATG CCTCATTGTT GAGAGAAATA 
CACAGAGACT ATGATGTGAG GAGAGTCCAG ATGAAACACA AGACCAAACT AGAATCTACA 
TTTAAACACA TTTTTGAAGG GATTGCAAAG CAGGGAAACC CAACCCTCCT GAACAGAATC 
TACACTGAGC TATACATCAC AGAGGGAGAG AGAGAAGGAG TCAATGATGA ACATGAGGTG 
TGGCAGATCG AGAGTGCTTA CAGGACACAC AGTTCACTAG ACACTCCTAT CAACTGCAAC 
GACATTTTCA AGTGCCTACC TGGTCAAGAG AAAACCATCA GAACTGTGCT GACCAAGGGC 
ATTGCTGGCA TTGGAAAAAC AGTTTCTGTG CAGAAGTTCA TCCTTGACTG GGCTGAAGGA 
AAAGCCAATC AGGACATTGA TTTCATTTTT GTTCTTCCTT TTCGGGAACT CAATTTAATT 
AAAGATGAGC AGTACAGTCT TCTTGAACTT CTTCATGAGT TCTATCCAGA AATAGAAAAT 
ATCAAAGACA TCAGATTTAA TAAATATAAA GTTATGCTGA TCTTTGATGG TCTGGATGAA 
AGCAGACTTC CGTTGAATTT TCATCACACG AGGTTGTCTG ATGTTAAGGA GGCCACATCA 
GTCGATGTGT TGTTGACCAA CCTCATTAAG AGGATTCTTC TTCCTTCTGC TCTCCTGTGG 
ATAACAACCA GGCCAGCAGC AGCCAGTCAG ATCCCTCCTG AGTATGTTGA CCAGGTGACA 
GAGATTCGGG GGTTTAATGA CCCACAAAAA GAGGAGTATT TTCATAAAAG ATTCAGTGAT 
GAGGATCTGG CCAACAGAAT TATATCTCAC ATACAGTCAT CAAGGAGCCT CTACATCATG 
TGTCACATAC CAGTTTTCTG TTGGATTTCA GCAACTGTTC TTGAGAAAAT GCTAGGTAAT 
GATGGTGGTG AAGAAATACC TACAACTCTG ACACAAATGT ATACACACTT CCTGCTCATT 
CAGACCAACA TAAAGAACAA GAAGTATCAT GGAACCAATG AGACCGACTC CAGAAACGTG 
TCACAGTCAG ACAGAGATCT TATACTTAAG TTGGCAGAGT TAGCTTTCCA CCAGCTGGAA 
AAGGGAAATA TCATATTTTA TGAAACGGAC TTGAAAGACT GTGACATTGA TGTCAGTGAA 
GCATCAGTGT ATTCTGGGGT GTGCACCCAA ATCTTCAAGG AGGAATCAGT GATGTATAAG 
AAGAAGGTGT ACTGCTTTGT GCATTTGAGC GTTCAGGAGT TTCTTGCTGC TCTGTATGTC 
ATCCACTGTC ATGCCAGCAA TAAAATGGAT TCCCTCAAAC TTTTCTCTGA AAGAAAGTCA 
AGAGTCTCAC ATCTCGAAAT GCTTTTGAAT GAGTTGCACA AACTCGCAGT TAACAAGGCC 
TTGGAAAGTA AGAATGGACA TCTGGACCTC TTCCTTCGCT TCCTTCTTGG CCTCTCAATG 
GACTCCAACA AGAGTCTCTT ACAAGGGTTG TTTACACAGA CTGAGAGCAG CTCAGAGAGC 
ATCAAGAAAA CAGTTAAATA CATTAAAGTA CTGAGGACGA AGTATCCTTC ACCTGAGAGG 
TATATGAATC TTTTCCTCTG TTTGGTTGAA TTGAATGACT TTTCCCTTCT GAAGAAAGTC 
CAGAAGTACC GGGACAGTTA TTCAGGGGAG AAACTCACTC CTGCAGAGTG TTCCTGTCTG 
GCCTATGTAC TCCTCATGTC GAATGAGGTA TTGGATGTGT TTGATATCTC AACATTCAAC 
ACGTCACCAG AGGGTCGTAT CAGACTCATC CCAGCTGTGA AGTGCTGCAG AAAGGCTGTA 
TTAACTCACT GTAAACTGAC AGGAGATTCC TGTGGATCTG TGGTCTCAGC TCTCCAGTCA 
GTGAACTCAC AGCTGACAGA ACTGGATCTC AGCTACAATC ACCTGGGGGA TTCAGGAGTG 
AAGGAGCTCT GTACTGGACT GATGAGTCCT CACTGTAAAC TACACACTCT GAGGTTGTCC 
CTGTGTAGAA TCACAGAGAG AGGCTGTTCT TCTCTGGCTT CAGCTCTGGG TTCGAACCCC 
CGCTCACAGC TGAGAGAGCT GGATCTGAGC TATAATCACC CAGGGGACTC AGGAGTGAAG 
CTGCTCTCTG CTCTACCGGA GAAAGTGAAT GTGGACCATG CTGGACAATG CAGGATCAGA 
CCAGGGCTCC TCAAGTACTC CTGCCAGTTC ACACTGGACC CCCACACAGC AAACAGAAAA 
CTAGTCCTGT CTGAGGACAA GAGGACAGTG ACATGGAGGG GAGAGGAACA AAAATATCCT 
GATCATCCAG AGAGATTTGA CTGTTTGAGA CAAGTTCTGT GTGTAGAGAG TGTGTCTCAT 
CGCTCTTACT GGGAGGTTCA GTGGACTGGA AATGAGGCTG AAATAGGAGT GACTTATAAA 
GGAATCAAGA GGAAAGGAGA TGGTGATGAC TGTCGGCTTG GATACAATGG CAAGTCCTGG 
GTTCTGTGCT GCACTGATAA GAGATACTCT GTGAGACACA ATAACACAAA CACTGCCATA 
GCTGCACCCC CCTCCCCCAG AGTAGGAGTG TATGTGGACT GTGTGTCCGG CACTCTGTCC 
TTCTACAGCG TCTCCTCTGG TGAACCGACC CTCCTGTACA GCTTCACCTC CACATTCACT 
GAGCCCCTCT ATCCTGTGTT TTCGCTTTGG GGTTCAGATT CTTCAGTGTC CCTGTGTGAC 
CTGGAATAG