rad26 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad26
Entrez Gene ID	2543289
Full Name	ATRIP, ATR checkpoint kinase regulatory subunit Rad26
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020010.2	NP_594581.1	DNA repair protein
NM_001356167.1	NP_001342867.1	ATR checkpoint kinase regulatory subunit Rad26

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate that Rad26(ATRIP) participates in two genetically defined checkpoint pathways--one that responds to genomic damage and the other to microtubule damage.
Title: Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage.

The N-terminus of Nbs1 contributes to the recruitment of Rad3(ATR)-Rad26(ATRIP) to telomeres.
Title: A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.

DNA replication is stalled leads to release of Rad3 and Rad26 from chromatin and entry into an aberrant mitosis.
Title: Cdc18 enforces long-term maintenance of the S phase checkpoint by anchoring the Rad3-Rad26 complex to chromatin.

data presented here show that two elements of fission yeast DNA structure checkpoints, Rad26 and Rad24, define a pathway that responds to microtubule destabilizing conditions
Title: The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization.

The following rad26 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad26 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj04449	NM_001020010.2 Latest version!	Schizosaccharomyces pombe 972h- DNA repair protein (rad26), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OSc102692	NM_001356167.1 Latest version!	Schizosaccharomyces pombe ATR checkpoint kinase regulatory subunit Rad26 (rad26), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad26 ( NM_001020010.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594581.1 Schizosaccharomyces pombe 972h- DNA repair protein (rad26), mRNA.

Price & Availability↑

CloneID	OSj04449
Clone ID Related Accession (Same CDS sequence)	NM_001020010.2
Accession Version	NM_001020010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	DNA repair protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020010.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGATGGCTG ATGAAAGTTT TGACTTGGAG AGTTTGGGTT CGGATGAAAT ATTTGAAGGC 
GTGAATTTAG ATGAATTAGA GCAACAAGCT CAAACCCAGG TTCAAGCTCA AAGTTCTCAA 
GTAGTTGTAC CGAGTGAAAA GCAAAAGCAA AATTTAAATC TTCCAAATTC ATACACCAAT 
TCTTCTCAAA AAGTCCGGGA ATCTACCGTA AACTCACAAG CCTCATTATC CTCTAATGAC 
CTTCGAACAG AACTTTTAAT TAAGTCGGGA GAAAATGCAA TTCTTCGTGC TAACCTTCTA 
AAACAATCCG AAGCTAATAA TGCCGCCCTA GAATCGTTAA ATAATTCTAT AAAGCAAAAG 
CAAGATGAAT ACCAACGGAA ATTAGAGGAG CTTAAAAAAG AAATTGAATA CGCAAAAACG 
AAATCCCTAT TTCATGAGCG TGAAGCACAG GATGCTATCG AAACTATGAA AAAAATGAAA 
AGAGATGTAA AGAATTCTCC GATTATGAAA AAATCCCATG AAGAAGATGG TGACAATAAA 
TTGTTATCTT CTTCTGATCA GCTTGCTAAG AGCACAAAGC ATGCTGCAAA AAACAGCCCT 
TCTAAAAAGA AGCGCAAAAC AAGTGTTGCT ACTGCTGAAG ATGCTTCAAC GGATAGTGTG 
AGTTCTAGTA TTGCTATATC AGATGCAAGT TTGTCTTTGA GCCTGATGAA AGATTTACTT 
TCTCTACAAA AAAGAGAAGA TTTGTATTTT TCTTCTAGAA CTTTAGCATA CGTATTTGGT 
GGTTGTATGC ATTCATTAGA AACAATTGAA GGAGAGGAGG AAGGTGAATG TTTGTTTAAT 
AACTTGAAAG CTCTAATATA CAGTCCCGAT CTATCTATGG ATTCCTCCAA TTATGTACAA 
TCTGTCGTTC AAACATCATC GTCGATTTTA AATTATTCAA TGAAAAAATT ATTATATAAT 
GCCTCCTTTG CTATAACATC TTTGTTTAAT GCATTATTAA TTTTGGACCC TAAATCATCC 
ACCTTTATCT TTCAGGAAAA TGTCGTTTCT TTAATAAGTG GTTTTCTTCT TAAGGAATAC 
GAAAAAAGCA ATTTTTTGGA CTCAAAGTTT TACGTTTTAA TAGATTTTCT TTATTTATAC 
TTATCTATAG CACGTGAAAG TGCTGATGAC TTTGCAAACA TTACTAAAGC GGTTGATCCG 
TCGTTATTCG AATCCTGCAT TCGAGTGCAA AATGCTCCTT CTTTGATTAA ATGTGGTGTT 
TGTTTAATTA TTAGTTCCAC CACTCCTTCG TTTTGCGCTT CAGTTAACTT GTTAAATGCC 
GATGATAAAT CCCAAGAATC ACTCATGCAA CTTTTCACAA CTATGGCCCA TATTTTAGTT 
GTTACTACTA GGGAAAGGAT TAATTTTCCT GAATTGAATG AATGGATAAC GCTTCATCGC 
TTTGTCATAT CCTTTTTTAC CGTGTTTATA CAAATGTCTG GAAACATTGG TAAAGAGATT 
TTAAAAGTAT GCAATCCCTT AATAGTATGC ATCGGACTAG CCATCACTTG GTATCATCAG 
CAACTCCTTT CGTCAATGTA CCCTCAAAAT GAATGCGTAG AGATTTTAGT ATCTCTTGTT 
CGGCTTCTGT ACATTTTATC TTCCGAAGAT TTATCATCAA AATTTATGTT AGCTGAAAAT 
GCGTTACAAC CTCGTTTCGT TTATGCAATA GCATGCTGCG CATTCGGCGA TACTGAACAA 
AAAGCGTTTG GCAATTTGGG TGAGGAAATG TATTTTCTCA CTACAGAATT GTTGGAAGTT 
TGCGTCTCTC CCGAAGAGCT GGAGCAGTTG TACACTAATT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594581.1
CDS	527..2371
Translation	MMADESFDLESLGSDEIFEGVNLDELEQQAQTQVQAQSSQVVVPSEKQKQNLNLPNSYTNSSQKVRESTVNSQASLSSNDLRTELLIKSGENAILRANLLKQSEANNAALESLNNSIKQKQDEYQRKLEELKKEIEYAKTKSLFHEREAQDAIETMKKMKRDVKNSPIMKKSHEEDGDNKLLSSSDQLAKSTKHAAKNSPSKKKRKTSVATAEDASTDSVSSSIAISDASLSLSLMKDLLSLQKREDLYFSSRTLAYVFGGCMHSLETIEGEEEGECLFNNLKALIYSPDLSMDSSNYVQSVVQTSSSILNYSMKKLLYNASFAITSLFNALLILDPKSSTFIFQENVVSLISGFLLKEYEKSNFLDSKFYVLIDFLYLYLSIARESADDFANITKAVDPSLFESCIRVQNAPSLIKCGVCLIISSTTPSFCASVNLLNADDKSQESLMQLFTTMAHILVVTTRERINFPELNEWITLHRFVISFFTVFIQMSGNIGKEILKVCNPLIVCIGLAITWYHQQLLSSMYPQNECVEILVSLVRLLYILSSEDLSSKFMLAENALQPRFVYAIACCAFGDTEQKAFGNLGEEMYFLTTELLEVCVSPEELEQLYTNF

Target ORF information:

RefSeq Version	NM_001020010.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- DNA repair protein (rad26), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020010.2

ORF Insert Sequence:

ATGATGGCTG ATGAAAGTTT TGACTTGGAG AGTTTGGGTT CGGATGAAAT ATTTGAAGGC 
GTGAATTTAG ATGAATTAGA GCAACAAGCT CAAACCCAGG TTCAAGCTCA AAGTTCTCAA 
GTAGTTGTAC CGAGTGAAAA GCAAAAGCAA AATTTAAATC TTCCAAATTC ATACACCAAT 
TCTTCTCAAA AAGTCCGGGA ATCTACCGTA AACTCACAAG CCTCATTATC CTCTAATGAC 
CTTCGAACAG AACTTTTAAT TAAGTCGGGA GAAAATGCAA TTCTTCGTGC TAACCTTCTA 
AAACAATCCG AAGCTAATAA TGCCGCCCTA GAATCGTTAA ATAATTCTAT AAAGCAAAAG 
CAAGATGAAT ACCAACGGAA ATTAGAGGAG CTTAAAAAAG AAATTGAATA CGCAAAAACG 
AAATCCCTAT TTCATGAGCG TGAAGCACAG GATGCTATCG AAACTATGAA AAAAATGAAA 
AGAGATGTAA AGAATTCTCC GATTATGAAA AAATCCCATG AAGAAGATGG TGACAATAAA 
TTGTTATCTT CTTCTGATCA GCTTGCTAAG AGCACAAAGC ATGCTGCAAA AAACAGCCCT 
TCTAAAAAGA AGCGCAAAAC AAGTGTTGCT ACTGCTGAAG ATGCTTCAAC GGATAGTGTG 
AGTTCTAGTA TTGCTATATC AGATGCAAGT TTGTCTTTGA GCCTGATGAA AGATTTACTT 
TCTCTACAAA AAAGAGAAGA TTTGTATTTT TCTTCTAGAA CTTTAGCATA CGTATTTGGT 
GGTTGTATGC ATTCATTAGA AACAATTGAA GGAGAGGAGG AAGGTGAATG TTTGTTTAAT 
AACTTGAAAG CTCTAATATA CAGTCCCGAT CTATCTATGG ATTCCTCCAA TTATGTACAA 
TCTGTCGTTC AAACATCATC GTCGATTTTA AATTATTCAA TGAAAAAATT ATTATATAAT 
GCCTCCTTTG CTATAACATC TTTGTTTAAT GCATTATTAA TTTTGGACCC TAAATCATCC 
ACCTTTATCT TTCAGGAAAA TGTCGTTTCT TTAATAAGTG GTTTTCTTCT TAAGGAATAC 
GAAAAAAGCA ATTTTTTGGA CTCAAAGTTT TACGTTTTAA TAGATTTTCT TTATTTATAC 
TTATCTATAG CACGTGAAAG TGCTGATGAC TTTGCAAACA TTACTAAAGC GGTTGATCCG 
TCGTTATTCG AATCCTGCAT TCGAGTGCAA AATGCTCCTT CTTTGATTAA ATGTGGTGTT 
TGTTTAATTA TTAGTTCCAC CACTCCTTCG TTTTGCGCTT CAGTTAACTT GTTAAATGCC 
GATGATAAAT CCCAAGAATC ACTCATGCAA CTTTTCACAA CTATGGCCCA TATTTTAGTT 
GTTACTACTA GGGAAAGGAT TAATTTTCCT GAATTGAATG AATGGATAAC GCTTCATCGC 
TTTGTCATAT CCTTTTTTAC CGTGTTTATA CAAATGTCTG GAAACATTGG TAAAGAGATT 
TTAAAAGTAT GCAATCCCTT AATAGTATGC ATCGGACTAG CCATCACTTG GTATCATCAG 
CAACTCCTTT CGTCAATGTA CCCTCAAAAT GAATGCGTAG AGATTTTAGT ATCTCTTGTT 
CGGCTTCTGT ACATTTTATC TTCCGAAGAT TTATCATCAA AATTTATGTT AGCTGAAAAT 
GCGTTACAAC CTCGTTTCGT TTATGCAATA GCATGCTGCG CATTCGGCGA TACTGAACAA 
AAAGCGTTTG GCAATTTGGG TGAGGAAATG TATTTTCTCA CTACAGAATT GTTGGAAGTT 
TGCGTCTCTC CCGAAGAGCT GGAGCAGTTG TACACTAATT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad26 ( NM_001356167.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342867.1 Schizosaccharomyces pombe ATR checkpoint kinase regulatory subunit Rad26 (rad26), mRNA.

Price & Availability↑

CloneID	OSc102692
Clone ID Related Accession (Same CDS sequence)	NM_001356167.1
Accession Version	NM_001356167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	ATR checkpoint kinase regulatory subunit Rad26
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGATGGCTG ATGAAAGTTT TGACTTGGAG AGTTTGGGTT CGGATGAAAT ATTTGAAGGC 
GTGAATTTAG ATGAATTAGA GCAACAAGCT CAAACCCAGG TTCAAGCTCA AAGTTCTCAA 
GTAGTTGTAC CGAGTGAAAA GCAAAAGCAA AATTTAAATC TTCCAAATTC ATACACCAAT 
TCTTCTCAAA AAGTCCGGGA ATCTACCGTA AACTCACAAG CCTCATTATC CTCTAATGAC 
CTTCGAACAG AACTTTTAAT TAAGTCGGGA GAAAATGCAA TTCTTCGTGC TAACCTTCTA 
AAACAATCCG AAGCTAATAA TGCCGCCCTA GAATCGTTAA ATAATTCTAT AAAGCAAAAG 
CAAGATGAAT ACCAACGGAA ATTAGAGGAG CTTAAAAAAG AAATTGAATA CGCAAAAACG 
AAATCCCTAT TTCATGAGCG TGAAGCACAG GATGCTATCG AAACTATGAA AAAAATGAAA 
AGAGATGTAA AGAATTCTCC GATTATGAAA AAATCCCATG AAGAAGATGG TGACAATAAA 
TTGTTATCTT CTTCTGATCA GCTTGCTAAG AGCACAAAGC ATGCTGCAAA AAACAGCCCT 
TCTAAAAAGA AGCGCAAAAC AAGTGTTGCT ACTGCTGAAG ATGCTTCAAC GGATAGTGTG 
AGTTCTAGTA TTGCTATATC AGATGCAAGT TTGTCTTTGA GCCTGATGAA AGATTTACTT 
TCTCTACAAA AAAGAGAAGA TTTGTATTTT TCTTCTAGAA CTTTAGCATA CGTATTTGGT 
GGTTGTATGC ATTCATTAGA AACAATTGAA GGAGAGGAGG AAGGTGAATG TTTGTTTAAT 
AACTTGAAAG CTCTAATATA CAGTCCCGAT CTATCTATGG ATTCCTCCAA TTATGTACAA 
TCTGTCGTTC AAACATCATC GTCGATTTTA AATTATTCAA TGAAAAAATT ATTATATAAT 
GCCTCCTTTG CTATAACATC TTTGTTTAAT GCATTATTAA TTTTGGACCC TAAATCATCC 
ACCTTTATCT TTCAGGAAAA TGTCGTTTCT TTAATAAGTG GTTTTCTTCT TAAGGAATAC 
GAAAAAAGCA ATTTTTTGGA CTCAAAGTTT TACGTTTTAA TAGATTTTCT TTATTTATAC 
TTATCTATAG CACGTGAAAG TGCTGATGAC TTTGCAAACA TTACTAAAGC GGTTGATCCG 
TCGTTATTCG AATCCTGCAT TCGAGTGCAA AATGCTCCTT CTTTGATTAA ATGTGGTGTT 
TGTTTAATTA TTAGTTCCAC CACTCCTTCG TTTTGCGCTT CAGTTAACTT GTTAAATGCC 
GATGATAAAT CCCAAGAATC ACTCATGCAA CTTTTCACAA CTATGGCCCA TATTTTAGTT 
GTTACTACTA GGGAAAGGAT TAATTTTCCT GAATTGAATG AATGGATAAC GCTTCATCGC 
TTTGTCATAT CCTTTTTTAC CGTGTTTATA CAAATGTCTG GAAACATTGG TAAAGAGATT 
TTAAAAGTAT GCAATCCCTT AATAGTATGC ATCGGACTAG CCATCACTTG GTATCATCAG 
CAACTCCTTT CGTCAATGTA CCCTCAAAAT GAATGCGTAG AGATTTTAGT ATCTCTTGTT 
CGGCTTCTGT ACATTTTATC TTCCGAAGAT TTATCATCAA AATTTATGTT AGCTGAAAAT 
GCGTTACAAC CTCGTTTCGT TTATGCAATA GCATGCTGCG CATTCGGCGA TACTGAACAA 
AAAGCGTTTG GCAATTTGGG TGAGGAAATG TATTTTCTCA CTACAGAATT GTTGGAAGTT 
TGCGTCTCTC CCGAAGAGCT GGAGCAGTTG TACACTAATT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342867.1
CDS	447..2291
Translation	MMADESFDLESLGSDEIFEGVNLDELEQQAQTQVQAQSSQVVVPSEKQKQNLNLPNSYTNSSQKVRESTVNSQASLSSNDLRTELLIKSGENAILRANLLKQSEANNAALESLNNSIKQKQDEYQRKLEELKKEIEYAKTKSLFHEREAQDAIETMKKMKRDVKNSPIMKKSHEEDGDNKLLSSSDQLAKSTKHAAKNSPSKKKRKTSVATAEDASTDSVSSSIAISDASLSLSLMKDLLSLQKREDLYFSSRTLAYVFGGCMHSLETIEGEEEGECLFNNLKALIYSPDLSMDSSNYVQSVVQTSSSILNYSMKKLLYNASFAITSLFNALLILDPKSSTFIFQENVVSLISGFLLKEYEKSNFLDSKFYVLIDFLYLYLSIARESADDFANITKAVDPSLFESCIRVQNAPSLIKCGVCLIISSTTPSFCASVNLLNADDKSQESLMQLFTTMAHILVVTTRERINFPELNEWITLHRFVISFFTVFIQMSGNIGKEILKVCNPLIVCIGLAITWYHQQLLSSMYPQNECVEILVSLVRLLYILSSEDLSSKFMLAENALQPRFVYAIACCAFGDTEQKAFGNLGEEMYFLTTELLEVCVSPEELEQLYTNF

Target ORF information:

RefSeq Version	NM_001356167.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe ATR checkpoint kinase regulatory subunit Rad26 (rad26), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356167.1

ORF Insert Sequence:

ATGATGGCTG ATGAAAGTTT TGACTTGGAG AGTTTGGGTT CGGATGAAAT ATTTGAAGGC 
GTGAATTTAG ATGAATTAGA GCAACAAGCT CAAACCCAGG TTCAAGCTCA AAGTTCTCAA 
GTAGTTGTAC CGAGTGAAAA GCAAAAGCAA AATTTAAATC TTCCAAATTC ATACACCAAT 
TCTTCTCAAA AAGTCCGGGA ATCTACCGTA AACTCACAAG CCTCATTATC CTCTAATGAC 
CTTCGAACAG AACTTTTAAT TAAGTCGGGA GAAAATGCAA TTCTTCGTGC TAACCTTCTA 
AAACAATCCG AAGCTAATAA TGCCGCCCTA GAATCGTTAA ATAATTCTAT AAAGCAAAAG 
CAAGATGAAT ACCAACGGAA ATTAGAGGAG CTTAAAAAAG AAATTGAATA CGCAAAAACG 
AAATCCCTAT TTCATGAGCG TGAAGCACAG GATGCTATCG AAACTATGAA AAAAATGAAA 
AGAGATGTAA AGAATTCTCC GATTATGAAA AAATCCCATG AAGAAGATGG TGACAATAAA 
TTGTTATCTT CTTCTGATCA GCTTGCTAAG AGCACAAAGC ATGCTGCAAA AAACAGCCCT 
TCTAAAAAGA AGCGCAAAAC AAGTGTTGCT ACTGCTGAAG ATGCTTCAAC GGATAGTGTG 
AGTTCTAGTA TTGCTATATC AGATGCAAGT TTGTCTTTGA GCCTGATGAA AGATTTACTT 
TCTCTACAAA AAAGAGAAGA TTTGTATTTT TCTTCTAGAA CTTTAGCATA CGTATTTGGT 
GGTTGTATGC ATTCATTAGA AACAATTGAA GGAGAGGAGG AAGGTGAATG TTTGTTTAAT 
AACTTGAAAG CTCTAATATA CAGTCCCGAT CTATCTATGG ATTCCTCCAA TTATGTACAA 
TCTGTCGTTC AAACATCATC GTCGATTTTA AATTATTCAA TGAAAAAATT ATTATATAAT 
GCCTCCTTTG CTATAACATC TTTGTTTAAT GCATTATTAA TTTTGGACCC TAAATCATCC 
ACCTTTATCT TTCAGGAAAA TGTCGTTTCT TTAATAAGTG GTTTTCTTCT TAAGGAATAC 
GAAAAAAGCA ATTTTTTGGA CTCAAAGTTT TACGTTTTAA TAGATTTTCT TTATTTATAC 
TTATCTATAG CACGTGAAAG TGCTGATGAC TTTGCAAACA TTACTAAAGC GGTTGATCCG 
TCGTTATTCG AATCCTGCAT TCGAGTGCAA AATGCTCCTT CTTTGATTAA ATGTGGTGTT 
TGTTTAATTA TTAGTTCCAC CACTCCTTCG TTTTGCGCTT CAGTTAACTT GTTAAATGCC 
GATGATAAAT CCCAAGAATC ACTCATGCAA CTTTTCACAA CTATGGCCCA TATTTTAGTT 
GTTACTACTA GGGAAAGGAT TAATTTTCCT GAATTGAATG AATGGATAAC GCTTCATCGC 
TTTGTCATAT CCTTTTTTAC CGTGTTTATA CAAATGTCTG GAAACATTGG TAAAGAGATT 
TTAAAAGTAT GCAATCCCTT AATAGTATGC ATCGGACTAG CCATCACTTG GTATCATCAG 
CAACTCCTTT CGTCAATGTA CCCTCAAAAT GAATGCGTAG AGATTTTAGT ATCTCTTGTT 
CGGCTTCTGT ACATTTTATC TTCCGAAGAT TTATCATCAA AATTTATGTT AGCTGAAAAT 
GCGTTACAAC CTCGTTTCGT TTATGCAATA GCATGCTGCG CATTCGGCGA TACTGAACAA 
AAAGCGTTTG GCAATTTGGG TGAGGAAATG TATTTTCTCA CTACAGAATT GTTGGAAGTT 
TGCGTCTCTC CCGAAGAGCT GGAGCAGTTG TACACTAATT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rad26 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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