SPAC139.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC139.01c
Entrez Gene ID	2541762
Full Name	nuclease, XP-G family (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001712994.2	XP_001713046.1	nuclease, XP-G family (predicted)
NM_001355998.1	NP_001342957.1	putative XP-G family nuclease

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_08303	XP_003715774.1
Saccharomyces cerevisiae (baker's yeast)	MKT1	NP_014314.3
Kluyveromyces lactis	KLLA0C13926g	XP_452822.1
Schizosaccharomyces pombe (fission yeast)	SPAC139.01c	XP_001713046.1
Neurospora crassa	NCU03662	XP_960742.1
Eremothecium gossypii	AGOS_AER414W	NP_985269.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC139.01c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC139.01c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj03011	XM_001712994.2 Latest version!	Schizosaccharomyces pombe 972h- nuclease, XP-G family (predicted) (SPAC139.01c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSc101783	NM_001355998.1 Latest version!	Schizosaccharomyces pombe putative XP-G family nuclease (SPAC139.01c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC139.01c ( XM_001712994.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713046.1 Schizosaccharomyces pombe 972h- nuclease, XP-G family (predicted) (SPAC139.01c), mRNA.

Price & Availability↑

CloneID	OSj03011
Clone ID Related Accession (Same CDS sequence)	XM_001712994.2
Accession Version	XM_001712994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	nuclease, XP-G family (predicted)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712994.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGACAATTC GTTCATTGAA TTTGTTTATC ATAGACAAAA AGCATCAACA TAAATCTAGC 
TTGAGCAGTT TTCAAAACTG TAAGTTGGGT ATTGACGCAA GTTTTTACTT GACTCAAATT 
ATTCATTCCT TTACCCCACA GGAACTTCAA AGTTTGGCAG TAAATGGTGA ATCCGAATAT 
CTACAACACA GAATTTCGGA ATTCCTTGAG CAGTTGAGAA CTGAGAATAT CACACCCATT 
TTTGTTTTCA ATGGTATACC TCTTACCTTT GAAGCCAGCA GCCAGCTGGA AGTGCCGGGA 
AAGCAAAAAA GTCATAGTGC ATTGACAGAT TTCGAAGCTT TTGATCCTTA TGATGCAAAT 
ATTCAGCGTA ATATGTACCG CATGGACGCA AGTGGTCCTG CGAATTACGG CGAAAGTAAA 
CCAACACTTT TGTACACCAA TCAGCGAGAT CATTTAGATC GTCTTTGCGA CCAGGTAAAG 
TTTTACTTAG ATCAATGTAA TGTGGAGTAT TTTGTCGCCC CGTATCTGGC TATGGCTCAA 
TTAGCATATT TTCTAAACGG CACATCTTCA CCTTACATCG ATGCCATCTA TGGTTCCACG 
GATCTGCTTC TTTTTGGTGT TAAAAAATTT ATTACTTCTA TGAACACATC TTCTAACGTT 
AAAATCTCAT CTGACCCTTC ATCCCCATCA ACTCAAACAA CTATCAATTC GGCCGCTAAA 
AGTTCATTCA CATGGTTAGA TGGAAATGCC CTTCTTCAAG ACACGAACGG CCTTTCATGG 
CAGCAGTTCA TCGATTCCTG TTTATTGTGT GGTACTGCTA TTTCTCCTAC ATTTCCTCAG 
ATTGAGGGTA CTTTTCTAAT TAAAAGCGCA ATGGAGCTTG TTCGTATGTT TGGTTCTGCG 
TATCGTGCAG TCCTTCATTT TGCTGAAATC TTTCCCCAGC CCATTTTTCA AGATTATCTT 
CAACAGTACA AAAGGGCTGT TTGTTTCTCT AAGTTTGGAA TTGTGATGGA TACAAAAGGT 
CTCACTTTAC CACCAGTTCC CACAGAATCT GTTCCTAACG ACATAAATAT ATATTTTGGC 
ACCCGTCTTC CCAACGAGAT TTATTTTTAC ATTTCTCGCG GTCTTATTCC TTGCAAAATG 
ATAGGTGCTT TAGTATCTGG ATGTTTTAGT GATCCTGTTT CCATACTTGA ACAACATATT 
GGTGACAAAG CATCACAAGG AACATTTTCT GGTGCTAATT CTGGTTTAAA TCCTGGTCGC 
GACAATGCAG CAGCTGTAGC GGCTGTGGAT CAACGGCGAT TTGTTGACGA TTTAGAAGAA 
ATATGGTCGC AGGGGCTAAA TTTATTGACC CAACCGCTTA ACAGATTTTA CCAAGCCAGA 
GATATTGTTT CCCTTCATGG CCATAATCAG CAAGCTTCTT TGAAGGTAAT GCACAGCTAT 
GATCCTCCTT TGTATAATGA TACGAGAGCA TGGATGATAT ACGAAGAAAA CTTACCGAGT 
TATTTATCTA GTAATTTTTT AAAGGAAGAA CCCGTTGTAT TATTTGATCT ACTACGTGCT 
TTGAACGATC CGGTATTTGT AAAGAAAAGC TTTTGCACTG AAGGTAATAT TGGCATAAAA 
AACCCTCCCA AGCATCCTCT GTCTTCTACT GCGGAAATCG TCTTAAGTTC TTGCTACAGA 
TTTTTGCAAA TTAGATCTTT CGTTTTAACA TCTCATCAAT TAACATCTTG GGGATGCCCC 
TTGTTAAAAG CGTTGGAAAA TTGTCACTTA CAGAACCAAA CTTCCGTTGT TGTTTTATTC 
GAGCTTCTTA GACTAAGACA ATTAAAAGAG CCCTCTTTAC TTTCCTCCTC AAGTGCAAGC 
ATCGCCGATT TATCCATAAC TTCTGCCACA GAGTTCTTGG CAAAGGTTGC AACATTTCTA 
CCAATAAAAC AGAGACCTGA AGTTAGTAAA ATTTCTATAG TTGATGAGAA TTTATTACAG 
TTTTATCAGC TACAAACTAG CTTCGGATCC AACTTGAAGG AACTCATGGC CATGATTCTT 
GCCTCGGTCA TCTTGAACAG AAACGTCGAC AAAAGCAAAA TAGATCCTAA ACTTATCCGA 
AAAACCTTAC CTTTTCAAAA CATAAATGGT AGCCTTGTCT CAGGTTTTGT TGTCAAGCGC 
TTTTTTGAAA TCATTTCAAA AGAGCAAGAA GCCAGTTCTC AACAGGAAAA CATCCAGAAG 
GCTTATGAAA TAATAGAAAA GGAATTCCCA ACCATTGGAT CTGCGGAAAA CCATATTGCC 
CAGTTTTTGG AATTCTGGAA AACTTTTATG GAAGGGGTTA AGGAAGCAGA AAATACAAGT 
GCCATTGGCA AGCTCGTTTT AAGTAAACTC TTCCTTACTA ACCAATGGAT ATTTTCTCTT 
GGCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713046.1
CDS	157..2565
Translation	MTIRSLNLFIIDKKHQHKSSLSSFQNCKLGIDASFYLTQIIHSFTPQELQSLAVNGESEYLQHRISEFLEQLRTENITPIFVFNGIPLTFEASSQLEVPGKQKSHSALTDFEAFDPYDANIQRNMYRMDASGPANYGESKPTLLYTNQRDHLDRLCDQVKFYLDQCNVEYFVAPYLAMAQLAYFLNGTSSPYIDAIYGSTDLLLFGVKKFITSMNTSSNVKISSDPSSPSTQTTINSAAKSSFTWLDGNALLQDTNGLSWQQFIDSCLLCGTAISPTFPQIEGTFLIKSAMELVRMFGSAYRAVLHFAEIFPQPIFQDYLQQYKRAVCFSKFGIVMDTKGLTLPPVPTESVPNDINIYFGTRLPNEIYFYISRGLIPCKMIGALVSGCFSDPVSILEQHIGDKASQGTFSGANSGLNPGRDNAAAVAAVDQRRFVDDLEEIWSQGLNLLTQPLNRFYQARDIVSLHGHNQQASLKVMHSYDPPLYNDTRAWMIYEENLPSYLSSNFLKEEPVVLFDLLRALNDPVFVKKSFCTEGNIGIKNPPKHPLSSTAEIVLSSCYRFLQIRSFVLTSHQLTSWGCPLLKALENCHLQNQTSVVVLFELLRLRQLKEPSLLSSSSASIADLSITSATEFLAKVATFLPIKQRPEVSKISIVDENLLQFYQLQTSFGSNLKELMAMILASVILNRNVDKSKIDPKLIRKTLPFQNINGSLVSGFVVKRFFEIISKEQEASSQQENIQKAYEIIEKEFPTIGSAENHIAQFLEFWKTFMEGVKEAENTSAIGKLVLSKLFLTNQWIFSLGL

Target ORF information:

RefSeq Version	XM_001712994.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- nuclease, XP-G family (predicted) (SPAC139.01c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001712994.2

ORF Insert Sequence:

ATGACAATTC GTTCATTGAA TTTGTTTATC ATAGACAAAA AGCATCAACA TAAATCTAGC 
TTGAGCAGTT TTCAAAACTG TAAGTTGGGT ATTGACGCAA GTTTTTACTT GACTCAAATT 
ATTCATTCCT TTACCCCACA GGAACTTCAA AGTTTGGCAG TAAATGGTGA ATCCGAATAT 
CTACAACACA GAATTTCGGA ATTCCTTGAG CAGTTGAGAA CTGAGAATAT CACACCCATT 
TTTGTTTTCA ATGGTATACC TCTTACCTTT GAAGCCAGCA GCCAGCTGGA AGTGCCGGGA 
AAGCAAAAAA GTCATAGTGC ATTGACAGAT TTCGAAGCTT TTGATCCTTA TGATGCAAAT 
ATTCAGCGTA ATATGTACCG CATGGACGCA AGTGGTCCTG CGAATTACGG CGAAAGTAAA 
CCAACACTTT TGTACACCAA TCAGCGAGAT CATTTAGATC GTCTTTGCGA CCAGGTAAAG 
TTTTACTTAG ATCAATGTAA TGTGGAGTAT TTTGTCGCCC CGTATCTGGC TATGGCTCAA 
TTAGCATATT TTCTAAACGG CACATCTTCA CCTTACATCG ATGCCATCTA TGGTTCCACG 
GATCTGCTTC TTTTTGGTGT TAAAAAATTT ATTACTTCTA TGAACACATC TTCTAACGTT 
AAAATCTCAT CTGACCCTTC ATCCCCATCA ACTCAAACAA CTATCAATTC GGCCGCTAAA 
AGTTCATTCA CATGGTTAGA TGGAAATGCC CTTCTTCAAG ACACGAACGG CCTTTCATGG 
CAGCAGTTCA TCGATTCCTG TTTATTGTGT GGTACTGCTA TTTCTCCTAC ATTTCCTCAG 
ATTGAGGGTA CTTTTCTAAT TAAAAGCGCA ATGGAGCTTG TTCGTATGTT TGGTTCTGCG 
TATCGTGCAG TCCTTCATTT TGCTGAAATC TTTCCCCAGC CCATTTTTCA AGATTATCTT 
CAACAGTACA AAAGGGCTGT TTGTTTCTCT AAGTTTGGAA TTGTGATGGA TACAAAAGGT 
CTCACTTTAC CACCAGTTCC CACAGAATCT GTTCCTAACG ACATAAATAT ATATTTTGGC 
ACCCGTCTTC CCAACGAGAT TTATTTTTAC ATTTCTCGCG GTCTTATTCC TTGCAAAATG 
ATAGGTGCTT TAGTATCTGG ATGTTTTAGT GATCCTGTTT CCATACTTGA ACAACATATT 
GGTGACAAAG CATCACAAGG AACATTTTCT GGTGCTAATT CTGGTTTAAA TCCTGGTCGC 
GACAATGCAG CAGCTGTAGC GGCTGTGGAT CAACGGCGAT TTGTTGACGA TTTAGAAGAA 
ATATGGTCGC AGGGGCTAAA TTTATTGACC CAACCGCTTA ACAGATTTTA CCAAGCCAGA 
GATATTGTTT CCCTTCATGG CCATAATCAG CAAGCTTCTT TGAAGGTAAT GCACAGCTAT 
GATCCTCCTT TGTATAATGA TACGAGAGCA TGGATGATAT ACGAAGAAAA CTTACCGAGT 
TATTTATCTA GTAATTTTTT AAAGGAAGAA CCCGTTGTAT TATTTGATCT ACTACGTGCT 
TTGAACGATC CGGTATTTGT AAAGAAAAGC TTTTGCACTG AAGGTAATAT TGGCATAAAA 
AACCCTCCCA AGCATCCTCT GTCTTCTACT GCGGAAATCG TCTTAAGTTC TTGCTACAGA 
TTTTTGCAAA TTAGATCTTT CGTTTTAACA TCTCATCAAT TAACATCTTG GGGATGCCCC 
TTGTTAAAAG CGTTGGAAAA TTGTCACTTA CAGAACCAAA CTTCCGTTGT TGTTTTATTC 
GAGCTTCTTA GACTAAGACA ATTAAAAGAG CCCTCTTTAC TTTCCTCCTC AAGTGCAAGC 
ATCGCCGATT TATCCATAAC TTCTGCCACA GAGTTCTTGG CAAAGGTTGC AACATTTCTA 
CCAATAAAAC AGAGACCTGA AGTTAGTAAA ATTTCTATAG TTGATGAGAA TTTATTACAG 
TTTTATCAGC TACAAACTAG CTTCGGATCC AACTTGAAGG AACTCATGGC CATGATTCTT 
GCCTCGGTCA TCTTGAACAG AAACGTCGAC AAAAGCAAAA TAGATCCTAA ACTTATCCGA 
AAAACCTTAC CTTTTCAAAA CATAAATGGT AGCCTTGTCT CAGGTTTTGT TGTCAAGCGC 
TTTTTTGAAA TCATTTCAAA AGAGCAAGAA GCCAGTTCTC AACAGGAAAA CATCCAGAAG 
GCTTATGAAA TAATAGAAAA GGAATTCCCA ACCATTGGAT CTGCGGAAAA CCATATTGCC 
CAGTTTTTGG AATTCTGGAA AACTTTTATG GAAGGGGTTA AGGAAGCAGA AAATACAAGT 
GCCATTGGCA AGCTCGTTTT AAGTAAACTC TTCCTTACTA ACCAATGGAT ATTTTCTCTT 
GGCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAC139.01c ( NM_001355998.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342957.1 Schizosaccharomyces pombe putative XP-G family nuclease (SPAC139.01c), mRNA.

Price & Availability↑

CloneID	OSc101783
Clone ID Related Accession (Same CDS sequence)	NM_001355998.1
Accession Version	NM_001355998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative XP-G family nuclease
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGACAATTC GTTCATTGAA TTTGTTTATC ATAGACAAAA AGCATCAACA TAAATCTAGC 
TTGAGCAGTT TTCAAAACTG TAAGTTGGGT ATTGACGCAA GTTTTTACTT GACTCAAATT 
ATTCATTCCT TTACCCCACA GGAACTTCAA AGTTTGGCAG TAAATGGTGA ATCCGAATAT 
CTACAACACA GAATTTCGGA ATTCCTTGAG CAGTTGAGAA CTGAGAATAT CACACCCATT 
TTTGTTTTCA ATGGTATACC TCTTACCTTT GAAGCCAGCA GCCAGCTGGA AGTGCCGGGA 
AAGCAAAAAA GTCATAGTGC ATTGACAGAT TTCGAAGCTT TTGATCCTTA TGATGCAAAT 
ATTCAGCGTA ATATGTACCG CATGGACGCA AGTGGTCCTG CGAATTACGG CGAAAGTAAA 
CCAACACTTT TGTACACCAA TCAGCGAGAT CATTTAGATC GTCTTTGCGA CCAGGTAAAG 
TTTTACTTAG ATCAATGTAA TGTGGAGTAT TTTGTCGCCC CGTATCTGGC TATGGCTCAA 
TTAGCATATT TTCTAAACGG CACATCTTCA CCTTACATCG ATGCCATCTA TGGTTCCACG 
GATCTGCTTC TTTTTGGTGT TAAAAAATTT ATTACTTCTA TGAACACATC TTCTAACGTT 
AAAATCTCAT CTGACCCTTC ATCCCCATCA ACTCAAACAA CTATCAATTC GGCCGCTAAA 
AGTTCATTCA CATGGTTAGA TGGAAATGCC CTTCTTCAAG ACACGAACGG CCTTTCATGG 
CAGCAGTTCA TCGATTCCTG TTTATTGTGT GGTACTGCTA TTTCTCCTAC ATTTCCTCAG 
ATTGAGGGTA CTTTTCTAAT TAAAAGCGCA ATGGAGCTTG TTCGTATGTT TGGTTCTGCG 
TATCGTGCAG TCCTTCATTT TGCTGAAATC TTTCCCCAGC CCATTTTTCA AGATTATCTT 
CAACAGTACA AAAGGGCTGT TTGTTTCTCT AAGTTTGGAA TTGTGATGGA TACAAAAGGT 
CTCACTTTAC CACCAGTTCC CACAGAATCT GTTCCTAACG ACATAAATAT ATATTTTGGC 
ACCCGTCTTC CCAACGAGAT TTATTTTTAC ATTTCTCGCG GTCTTATTCC TTGCAAAATG 
ATAGGTGCTT TAGTATCTGG ATGTTTTAGT GATCCTGTTT CCATACTTGA ACAACATATT 
GGTGACAAAG CATCACAAGG AACATTTTCT GGTGCTAATT CTGGTTTAAA TCCTGGTCGC 
GACAATGCAG CAGCTGTAGC GGCTGTGGAT CAACGGCGAT TTGTTGACGA TTTAGAAGAA 
ATATGGTCGC AGGGGCTAAA TTTATTGACC CAACCGCTTA ACAGATTTTA CCAAGCCAGA 
GATATTGTTT CCCTTCATGG CCATAATCAG CAAGCTTCTT TGAAGGTAAT GCACAGCTAT 
GATCCTCCTT TGTATAATGA TACGAGAGCA TGGATGATAT ACGAAGAAAA CTTACCGAGT 
TATTTATCTA GTAATTTTTT AAAGGAAGAA CCCGTTGTAT TATTTGATCT ACTACGTGCT 
TTGAACGATC CGGTATTTGT AAAGAAAAGC TTTTGCACTG AAGGTAATAT TGGCATAAAA 
AACCCTCCCA AGCATCCTCT GTCTTCTACT GCGGAAATCG TCTTAAGTTC TTGCTACAGA 
TTTTTGCAAA TTAGATCTTT CGTTTTAACA TCTCATCAAT TAACATCTTG GGGATGCCCC 
TTGTTAAAAG CGTTGGAAAA TTGTCACTTA CAGAACCAAA CTTCCGTTGT TGTTTTATTC 
GAGCTTCTTA GACTAAGACA ATTAAAAGAG CCCTCTTTAC TTTCCTCCTC AAGTGCAAGC 
ATCGCCGATT TATCCATAAC TTCTGCCACA GAGTTCTTGG CAAAGGTTGC AACATTTCTA 
CCAATAAAAC AGAGACCTGA AGTTAGTAAA ATTTCTATAG TTGATGAGAA TTTATTACAG 
TTTTATCAGC TACAAACTAG CTTCGGATCC AACTTGAAGG AACTCATGGC CATGATTCTT 
GCCTCGGTCA TCTTGAACAG AAACGTCGAC AAAAGCAAAA TAGATCCTAA ACTTATCCGA 
AAAACCTTAC CTTTTCAAAA CATAAATGGT AGCCTTGTCT CAGGTTTTGT TGTCAAGCGC 
TTTTTTGAAA TCATTTCAAA AGAGCAAGAA GCCAGTTCTC AACAGGAAAA CATCCAGAAG 
GCTTATGAAA TAATAGAAAA GGAATTCCCA ACCATTGGAT CTGCGGAAAA CCATATTGCC 
CAGTTTTTGG AATTCTGGAA AACTTTTATG GAAGGGGTTA AGGAAGCAGA AAATACAAGT 
GCCATTGGCA AGCTCGTTTT AAGTAAACTC TTCCTTACTA ACCAATGGAT ATTTTCTCTT 
GGCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342957.1
CDS	157..2565
Translation	MTIRSLNLFIIDKKHQHKSSLSSFQNCKLGIDASFYLTQIIHSFTPQELQSLAVNGESEYLQHRISEFLEQLRTENITPIFVFNGIPLTFEASSQLEVPGKQKSHSALTDFEAFDPYDANIQRNMYRMDASGPANYGESKPTLLYTNQRDHLDRLCDQVKFYLDQCNVEYFVAPYLAMAQLAYFLNGTSSPYIDAIYGSTDLLLFGVKKFITSMNTSSNVKISSDPSSPSTQTTINSAAKSSFTWLDGNALLQDTNGLSWQQFIDSCLLCGTAISPTFPQIEGTFLIKSAMELVRMFGSAYRAVLHFAEIFPQPIFQDYLQQYKRAVCFSKFGIVMDTKGLTLPPVPTESVPNDINIYFGTRLPNEIYFYISRGLIPCKMIGALVSGCFSDPVSILEQHIGDKASQGTFSGANSGLNPGRDNAAAVAAVDQRRFVDDLEEIWSQGLNLLTQPLNRFYQARDIVSLHGHNQQASLKVMHSYDPPLYNDTRAWMIYEENLPSYLSSNFLKEEPVVLFDLLRALNDPVFVKKSFCTEGNIGIKNPPKHPLSSTAEIVLSSCYRFLQIRSFVLTSHQLTSWGCPLLKALENCHLQNQTSVVVLFELLRLRQLKEPSLLSSSSASIADLSITSATEFLAKVATFLPIKQRPEVSKISIVDENLLQFYQLQTSFGSNLKELMAMILASVILNRNVDKSKIDPKLIRKTLPFQNINGSLVSGFVVKRFFEIISKEQEASSQQENIQKAYEIIEKEFPTIGSAENHIAQFLEFWKTFMEGVKEAENTSAIGKLVLSKLFLTNQWIFSLGL

Target ORF information:

RefSeq Version	NM_001355998.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative XP-G family nuclease (SPAC139.01c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355998.1

ORF Insert Sequence:

ATGACAATTC GTTCATTGAA TTTGTTTATC ATAGACAAAA AGCATCAACA TAAATCTAGC 
TTGAGCAGTT TTCAAAACTG TAAGTTGGGT ATTGACGCAA GTTTTTACTT GACTCAAATT 
ATTCATTCCT TTACCCCACA GGAACTTCAA AGTTTGGCAG TAAATGGTGA ATCCGAATAT 
CTACAACACA GAATTTCGGA ATTCCTTGAG CAGTTGAGAA CTGAGAATAT CACACCCATT 
TTTGTTTTCA ATGGTATACC TCTTACCTTT GAAGCCAGCA GCCAGCTGGA AGTGCCGGGA 
AAGCAAAAAA GTCATAGTGC ATTGACAGAT TTCGAAGCTT TTGATCCTTA TGATGCAAAT 
ATTCAGCGTA ATATGTACCG CATGGACGCA AGTGGTCCTG CGAATTACGG CGAAAGTAAA 
CCAACACTTT TGTACACCAA TCAGCGAGAT CATTTAGATC GTCTTTGCGA CCAGGTAAAG 
TTTTACTTAG ATCAATGTAA TGTGGAGTAT TTTGTCGCCC CGTATCTGGC TATGGCTCAA 
TTAGCATATT TTCTAAACGG CACATCTTCA CCTTACATCG ATGCCATCTA TGGTTCCACG 
GATCTGCTTC TTTTTGGTGT TAAAAAATTT ATTACTTCTA TGAACACATC TTCTAACGTT 
AAAATCTCAT CTGACCCTTC ATCCCCATCA ACTCAAACAA CTATCAATTC GGCCGCTAAA 
AGTTCATTCA CATGGTTAGA TGGAAATGCC CTTCTTCAAG ACACGAACGG CCTTTCATGG 
CAGCAGTTCA TCGATTCCTG TTTATTGTGT GGTACTGCTA TTTCTCCTAC ATTTCCTCAG 
ATTGAGGGTA CTTTTCTAAT TAAAAGCGCA ATGGAGCTTG TTCGTATGTT TGGTTCTGCG 
TATCGTGCAG TCCTTCATTT TGCTGAAATC TTTCCCCAGC CCATTTTTCA AGATTATCTT 
CAACAGTACA AAAGGGCTGT TTGTTTCTCT AAGTTTGGAA TTGTGATGGA TACAAAAGGT 
CTCACTTTAC CACCAGTTCC CACAGAATCT GTTCCTAACG ACATAAATAT ATATTTTGGC 
ACCCGTCTTC CCAACGAGAT TTATTTTTAC ATTTCTCGCG GTCTTATTCC TTGCAAAATG 
ATAGGTGCTT TAGTATCTGG ATGTTTTAGT GATCCTGTTT CCATACTTGA ACAACATATT 
GGTGACAAAG CATCACAAGG AACATTTTCT GGTGCTAATT CTGGTTTAAA TCCTGGTCGC 
GACAATGCAG CAGCTGTAGC GGCTGTGGAT CAACGGCGAT TTGTTGACGA TTTAGAAGAA 
ATATGGTCGC AGGGGCTAAA TTTATTGACC CAACCGCTTA ACAGATTTTA CCAAGCCAGA 
GATATTGTTT CCCTTCATGG CCATAATCAG CAAGCTTCTT TGAAGGTAAT GCACAGCTAT 
GATCCTCCTT TGTATAATGA TACGAGAGCA TGGATGATAT ACGAAGAAAA CTTACCGAGT 
TATTTATCTA GTAATTTTTT AAAGGAAGAA CCCGTTGTAT TATTTGATCT ACTACGTGCT 
TTGAACGATC CGGTATTTGT AAAGAAAAGC TTTTGCACTG AAGGTAATAT TGGCATAAAA 
AACCCTCCCA AGCATCCTCT GTCTTCTACT GCGGAAATCG TCTTAAGTTC TTGCTACAGA 
TTTTTGCAAA TTAGATCTTT CGTTTTAACA TCTCATCAAT TAACATCTTG GGGATGCCCC 
TTGTTAAAAG CGTTGGAAAA TTGTCACTTA CAGAACCAAA CTTCCGTTGT TGTTTTATTC 
GAGCTTCTTA GACTAAGACA ATTAAAAGAG CCCTCTTTAC TTTCCTCCTC AAGTGCAAGC 
ATCGCCGATT TATCCATAAC TTCTGCCACA GAGTTCTTGG CAAAGGTTGC AACATTTCTA 
CCAATAAAAC AGAGACCTGA AGTTAGTAAA ATTTCTATAG TTGATGAGAA TTTATTACAG 
TTTTATCAGC TACAAACTAG CTTCGGATCC AACTTGAAGG AACTCATGGC CATGATTCTT 
GCCTCGGTCA TCTTGAACAG AAACGTCGAC AAAAGCAAAA TAGATCCTAA ACTTATCCGA 
AAAACCTTAC CTTTTCAAAA CATAAATGGT AGCCTTGTCT CAGGTTTTGT TGTCAAGCGC 
TTTTTTGAAA TCATTTCAAA AGAGCAAGAA GCCAGTTCTC AACAGGAAAA CATCCAGAAG 
GCTTATGAAA TAATAGAAAA GGAATTCCCA ACCATTGGAT CTGCGGAAAA CCATATTGCC 
CAGTTTTTGG AATTCTGGAA AACTTTTATG GAAGGGGTTA AGGAAGCAGA AAATACAAGT 
GCCATTGGCA AGCTCGTTTT AAGTAAACTC TTCCTTACTA ACCAATGGAT ATTTTCTCTT 
GGCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC139.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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