SPAC12B10.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC12B10.01c
Entrez Gene ID	2541575
Full Name	ubiquitin-protein ligase E3 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020061.2	NP_594633.2	putative ubiquitin-protein ligase E3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UFD4	NP_012915.3
Magnaporthe oryzae (rice blast fungus)	MGG_03841	XP_003720049.1
Kluyveromyces lactis	KLLA0B13585g	XP_452136.1
Neurospora crassa	NCU09866	XP_960183.1
Schizosaccharomyces pombe (fission yeast)	SPAC12B10.01c	NP_594633.2
Eremothecium gossypii	AGOS_ABR013W	NP_982959.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC12B10.01c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC12B10.01c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102068	NM_001020061.2 Latest version!	Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPAC12B10.01c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC12B10.01c ( NM_001020061.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594633.2 Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPAC12B10.01c), mRNA.

Price & Availability↑

CloneID	OSc102068
Clone ID Related Accession (Same CDS sequence)	NM_001020061.2
Accession Version	NM_001020061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative ubiquitin-protein ligase E3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020061.1.

ATGTCTGTTC AAGCGTATAC GAATACTCCG AATTTACCGA ATTTAATACA TAGTGAATCT 
ATAACCAACT CACCTGTGTT TATACCAAAC CTACGAAGTA AGTCCGACAA CAAACATAGA 
TTAAACACTT CTGAAGAATT GCCATTTACA AAGAAACGCA GAGCAACTAA ACATTTTTTA 
AAAATGGAGG TATCTGCCAA AATTTCTGAA CCACCTTCTA GTAAAAGTGT GGAATTTGGA 
AGGCATTTAA ATATGCGGCA TGATTTTTCC GAATTGCTTG AACAACCACT TACAACGAGC 
AAAACTAGGG TCTTTAAATC CTTTAATGAA AAGCTCAAAG GTAAACCCCA GTTAGTGAAA 
TCCGATAAAG GTGTGTTGCC TCTTAATAAG CAATCGGATA TACTGAATTC TTATGAACGC 
GTTTTAAGCA ATGCTTCGGT TGATTCTGCA TTAGACAAAA AGGCGGAAGT TGATATCATT 
CCATTTGATA AATCAGTTAT GGAACCAAAG AACATAACTT TTAATGACAA AAGTGGCTTA 
ACCAAGTTTG GGAATTCAAA CTCTTATGAA ACTACGTATT CTGACTCCAG CAACTATCAT 
ACTTCTACTG ATTCGTCCCA ATATAATGAC CAAGATGACC ACGTCTATGA TGATACTAAT 
GATGGAACTG ATGATGATAT AAATAATAAC GAGTACAAAG ACGATGAATC CAGTAGTGGC 
AGTGAAAGTT ATGAAAGAGA TGAAGATGTT GATGAAGAGG AGGAAGAAGA CGATGATGAA 
AACAATGATG AGGGAGACGA TGAAGATGAA AATGAAAATG ACGAGTTAAG AAGCGAAAAC 
GGTTCACCTA ATCCTTCAGC CGCTGTTATT AATGAACAAG AAAACATGAA CATGAGTGGA 
ATCGAAGAAT ACGATGAAAC TAACTTGTTA AATGAACTGG GAAGAATCAG AAGAGGTAAT 
CAGTTTCAAA GTTTAGCGTC TTCTATACTT TCTTCCGGTA ATTTGGAGAA CTCTGATGAC 
GAAGATGTTT CTTTAGGTCC ACAGACATAC CGTTTCAATA GTTCCTTTCA TCCTTTTCGT 
TCTGCATTTT CTGCTCCAGG TGTTCAATTT GGACGCTTGT TAGAAGGAAT AAAGGATTTT 
AGTGATCCTA CTGTTCAAAT GCTTTCTCTT CAAGAACTTT CCGAAGCGTT TGCTATGAGT 
ACTGAAGATA TGCTTGTTGG ATTGTTTTCA ACCGATTCAT ACATTGCGGC TTTTTCCGAA 
ATCTTATCTG GGCGAAATTA TGATTTCGGA GAAGTCTCTA TTCAACTTAT GCTATCCTGT 
ACTACCTGTG TATCTAATAT GATGGAAGCT TTGCCTTTAT GTATGGCTAA AATAGCATAT 
AGTCCTATTG TTCGCATTCT GTGTGAAAGA ATGTTTGATA TGCAATACAT TGATATTGCT 
GAACAAGCTT TAGGAGTTTT AGAACGGCTT TCTAAAGATT TTGGTATTTG TATTTTGGAA 
CACAGAGGAA TGTTAGCTGC TCTCCAATAC TTTGATTTTT TTTATACTAC AGTTCAACGG 
ACAGCTATTT CTCTGGCTGC TAATTGCTGT AAGTTTTTGG ATGAATCCAA TGCTTCCGCA 
GCAGAAGAAA TTATTCCATT GCTTTCTAAT ATTCTACAAT CTTCTGATAC CATAGTCGTT 
AGTAAGGCAT ATTCTTGCCT GGAGACAATT ATTGAATCTT TGAAAACTTC ACCAAACATA 
ATTGAAACGA TAATTTCCGA GGATTTGATC ACCACTATTG TCAATGCTTT GACTAATTCT 
ACCTCACAAA ATAAGTCAAT GCACTTACAA GTGCAGCTAT TACATATAAT ATCATCTTTG 
TGTCAGTCAT CTTCTGCTTT GATTTTACCT TTTCTTAACC ATAATTTGCC GGATGTGATG 
TACGAAATGC TGTGTGGAAT ACCACCTTCT GATACATCCC ATCAGGCTGA TATGATTACA 
ATGCAAAGTT TGTATTATTG TCCGATCGAG TTAATAGAAA ACTTGTTAAG AGCTATAACT 
TCTTTGCTTC CTAAAAACAC TAGTAACTTG TCTGATGAAT TTAACACGAA ACTATATCGA 
TTAAACAGTA TACTCTTAAC TGTTGTAATG GACATTTATT TTATTGTGCC CCTTCATGAT 
ATCCGATCGT TAGCTGTGAC TACGGCATTG AAAATGTTAT GTTCAATTAG GGAAAATAAT 
TTAGATGGCT TAGTTTGTTC TCTTCCTTTA TCATCATTCA TTGCTTCAAT TTTAAATTCA 
CGCAAATCTG ACAATTTCTT GAAGCGAGAT GCTTTAGAAA TGTGTCTATT TTTGCTTGAA 
GCACTTCCTA ATGTTTATTC TTCGTTATTT ATACGAGAAG GCGTTGTACA GGAGGTTGGA 
TTTTTAGTTC GCTCTACCAA TGCAGACATG AAGAAAATAA AGTTAAGTAT TTCCTTTTCA 
CAAAACAAAT CTGCTGCTCG TCATGAGGAG TTGAAGAATT TATCGACGTT AAAGAGCCTT 
GCCAAGGAGT TTCTCTCTAA TTATAAGGAA GAAAATTTAG AAAACTCAAC ATTAGTTCAA 
CTTAAACAAT TGTCTAAACA TTTATTAAGT GAGACCAAAC AAGACGAATC TTTTTCAGAA 
CTAGCCAAAA TATTTCAAGA AGGATCTAAT ATTACTAGTC ATGAATTACT TCATTCTGGA 
TTAATTCATA ATTTGCTTTT ATCACTCAAA AAATTTGGAA GTTCATCTCT TCGAACATTT 
TTGTTAGCTA TGAATACATG TGATGAACGC GAAGTTTTAG AATTTGGTAA TGGTCCCTTA 
GTTTCACTTA TCTTTTGTTT GCAAAATCTA CTTTCAACTG TTGAGAATTT TCAACTTTCA 
ACGTTACCCC CAGATACTGA GAATGCGGTA GATCATGTTT TTTCGCGGCA GTTTAAACTT 
CGTTTAATGG CTCTTCCGGG ATCAAGAATT CGACCACCTT TCCGATCTCT TGTCGTTTCT 
ATTAATGGGC TAGCTACTAT TCGTACACTT GATAATTATC TTCACAGTCG CATTTCTGTG 
CGTAATGAAA CTGGACGTCG TTTTTCAATC CTCCGCGAGG CCGGTTCTTT GAGGGAGTCA 
ATGAGTGGAT CATCTCGTAA CTCTTCAGGA GACTATACTG ACTCAATGTC TCAAGATGCT 
CCTAATCACA CAACAGAGCC ATCAGAGCGA CGTGACTCCT CGACATCATC ACATTTCGAG 
GAACATTTTG TATTCAGTTT GATGGGTAAA AAGGTACCCC GAAACAAAAC AATATTCAGA 
ATTTTATATG AGTATATACA GTTGTCTGAC GATCATACTC TCGATGATTT TTGGAAAACT 
CCTGTCCCAA TATTTTATGG TGAGCCTGAT TGCATTCACA ATGATATGAA AGGGGAGTTA 
AACTATGAGA ATGAAACAGA GGGATTTTCT ATAAATATTA GAGAAATATT GGATTTATTG 
AGTATTTTGT ATTATGGGAT TAGAGACGTT CATACCCTCT TTCCTGACAA ACATTTCCGT 
GGCAACATTG AAAACATTCT TACAGATTTT AGCAACTGGA AGTTGAGTGC CAAATTGAAT 
AGACAACTTG AAGAGCAACA ACTTGTTGTT CACGGTTGTT TACCCTCCTG GTGTATATCA 
CTAACCTCTG CATATCCATT CCTAGTACCG TTTGAAACTC GATATCTTTT ATTGCAATCT 
ACTGCTTTTG GTTTATCAAG ATCCGTTTCT TTTCTTTTGT CCCGAAATCC TGAGCTATCA 
AAAAACGAAA GTTCCTCCAT ACTTCAATTT GTCAGCTTAC ATCGCCAGAA AATTCGTATT 
TCTCGGAAAA AAATATTTAA TTACGCTTTG CATTTGCTTG CAACTTATGC GGCTTCTGAA 
AATATTCTTG AAATAGAATA CGAAGATGAA GTTGGTTCAG GTCTTGGGCC CACTTTGGAG 
TTCTATACTT CAGTATCTAA AGAATTTACC TTAAATTCTC TCGATATTTG GCGGAATGAT 
CAACCCAACT CTAAATTTGT GTACCAAGCG TCTGGACTTT TTCCTTCTCC TATTCCATTA 
TTAGGGTCCA GTCCAGAAAA TGAAAGAAAA ATTTCGTTGT TCTTTGCTCT TGGACAGTTC 
GTTGCACGCT CGATTTATGA TTCAAGGATA ATAAGCATTC AGTTTAATCC ACTTTTCTTT 
GCACGAAATA TACCTTTAAC CATTTCCTCT GTAGCGAAAG TTGACAAGGG CTTAGCTAAC 
TCGTTACGAT ATTTAGAGAA ATTAATACCG GGTAAAAACC CTACAAACGC CGAGACGGAC 
ATTAAGCTTG AAGACCTTCA TCTCGATTTT ACTCTACCGG GATTTCCGTC TATTGAACTC 
ATACCTGATG GAGCTAGTAC ACCTGTGACA ACATTCAATG TGAGTGATTA TTTAAATTAT 
GTCATTGACT ATACTGTTGG AAAGGGTGTT CAACAACAAT TAGAAGCATT TCAAAATGGG 
TTTTCCAGCG TTTTTCCTTA TACTTCTTTG CAAGTTTTGA CAGAACATGA ATTGGTCACA 
CTATTCGGTA CTGTCGATGA GGATTGGAGT TACGCGACCT TGATGAAATC TATAGTAGCA 
GATCACGGTT ACACCATGGA AAGTCCTACA ATCCAGAGAT TACTGACTTT GATGAGTCAA 
ATGAATTTTC AAGAACAAAG AGACTTTTTG CAATTTATTA CTGGAAGCCG AAAACTTCCT 
ATTGGAGGAT TTGCTGGCCT AAATCCCCCG TTAACTGTTG TTAGGCGCTT AAATGAACCT 
CCTTATGTAC CTGATGATTA TCTTCCAAGC GTGATGACTT GTGTGAATTA TTTGAAACTA 
CCTGAGTATT CTTCAAGTGA GGTTTTAGGA AGTCGACTTT CTAAGGCTAT TTTAGAAGGA 
CAGGGCAGTT TTCATTTATC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594633.2
CDS	216..5159
Translation	MSVQAYTNTPNLPNLIHSESITNSPVFIPNLRSKSDNKHRLNTSEELPFTKKRRATKHFLKMEVSAKISEPPSSKSVEFGRHLNMRHDFSELLEQPLTTSKTRVFKSFNEKLKGKPQLVKSDKGVLPLNKQSDILNSYERVLSNASVDSALDKKAEVDIIPFDKSVMEPKNITFNDKSGLTKFGNSNSYETTYSDSSNYHTSTDSSQYNDQDDHVYDDTNDGTDDDINNNEYKDDESSSGSESYERDEDVDEEEEEDDDENNDEGDDEDENENDELRSENGSPNPSAAVINEQENMNMSGIEEYDETNLLNELGRIRRGNQFQSLASSILSSGNLENSDDEDVSLGPQTYRFNSSFHPFRSAFSAPGVQFGRLLEGIKDFSDPTVQMLSLQELSEAFAMSTEDMLVGLFSTDSYIAAFSEILSGRNYDFGEVSIQLMLSCTTCVSNMMEALPLCMAKIAYSPIVRILCERMFDMQYIDIAEQALGVLERLSKDFGICILEHRGMLAALQYFDFFYTTVQRTAISLAANCCKFLDESNASAAEEIIPLLSNILQSSDTIVVSKAYSCLETIIESLKTSPNIIETIISEDLITTIVNALTNSTSQNKSMHLQVQLLHIISSLCQSSSALILPFLNHNLPDVMYEMLCGIPPSDTSHQADMITMQSLYYCPIELIENLLRAITSLLPKNTSNLSDEFNTKLYRLNSILLTVVMDIYFIVPLHDIRSLAVTTALKMLCSIRENNLDGLVCSLPLSSFIASILNSRKSDNFLKRDALEMCLFLLEALPNVYSSLFIREGVVQEVGFLVRSTNADMKKIKLSISFSQNKSAARHEELKNLSTLKSLAKEFLSNYKEENLENSTLVQLKQLSKHLLSETKQDESFSELAKIFQEGSNITSHELLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDEREVLEFGNGPLVSLIFCLQNLLSTVENFQLSTLPPDTENAVDHVFSRQFKLRLMALPGSRIRPPFRSLVVSINGLATIRTLDNYLHSRISVRNETGRRFSILREAGSLRESMSGSSRNSSGDYTDSMSQDAPNHTTEPSERRDSSTSSHFEEHFVFSLMGKKVPRNKTIFRILYEYIQLSDDHTLDDFWKTPVPIFYGEPDCIHNDMKGELNYENETEGFSINIREILDLLSILYYGIRDVHTLFPDKHFRGNIENILTDFSNWKLSAKLNRQLEEQQLVVHGCLPSWCISLTSAYPFLVPFETRYLLLQSTAFGLSRSVSFLLSRNPELSKNESSSILQFVSLHRQKIRISRKKIFNYALHLLATYAASENILEIEYEDEVGSGLGPTLEFYTSVSKEFTLNSLDIWRNDQPNSKFVYQASGLFPSPIPLLGSSPENERKISLFFALGQFVARSIYDSRIISIQFNPLFFARNIPLTISSVAKVDKGLANSLRYLEKLIPGKNPTNAETDIKLEDLHLDFTLPGFPSIELIPDGASTPVTTFNVSDYLNYVIDYTVGKGVQQQLEAFQNGFSSVFPYTSLQVLTEHELVTLFGTVDEDWSYATLMKSIVADHGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPPLTVVRRLNEPPYVPDDYLPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS

Target ORF information:

RefSeq Version	NM_001020061.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPAC12B10.01c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020061.2

ORF Insert Sequence:

ATGTCTGTTC AAGCGTATAC GAATACTCCG AATTTACCGA ATTTAATACA TAGTGAATCT 
ATAACCAACT CACCTGTGTT TATACCAAAC CTACGAAGTA AGTCCGACAA CAAACATAGA 
TTAAACACTT CTGAAGAATT GCCATTTACA AAGAAACGCA GAGCAACTAA ACATTTTTTA 
AAAATGGAGG TATCTGCCAA AATTTCTGAA CCACCTTCTA GTAAAAGTGT GGAATTTGGA 
AGGCATTTAA ATATGCGGCA TGATTTTTCC GAATTGCTTG AACAACCACT TACAACGAGC 
AAAACTAGGG TCTTTAAATC CTTTAATGAA AAGCTCAAAG GTAAACCCCA GTTAGTGAAA 
TCCGATAAAG GTGTGTTGCC TCTTAATAAG CAATCGGATA TACTGAATTC TTATGAACGC 
GTTTTAAGCA ATGCTTCGGT TGATTCTGCA TTAGACAAAA AGGCGGAAGT TGATATCATT 
CCATTTGATA AATCAGTTAT GGAACCAAAG AACATAACTT TTAATGACAA AAGTGGCTTA 
ACCAAGTTTG GGAATTCAAA CTCTTATGAA ACTACGTATT CTGACTCCAG CAACTATCAT 
ACTTCTACTG ATTCGTCCCA ATATAATGAC CAAGATGACC ACGTCTATGA TGATACTAAT 
GATGGAACTG ATGATGATAT AAATAATAAC GAGTACAAAG ACGATGAATC CAGTAGTGGC 
AGTGAAAGTT ATGAAAGAGA TGAAGATGTT GATGAAGAGG AGGAAGAAGA CGATGATGAA 
AACAATGATG AGGGAGACGA TGAAGATGAA AATGAAAATG ACGAGTTAAG AAGCGAAAAC 
GGTTCACCTA ATCCTTCAGC CGCTGTTATT AATGAACAAG AAAACATGAA CATGAGTGGA 
ATCGAAGAAT ACGATGAAAC TAACTTGTTA AATGAACTGG GAAGAATCAG AAGAGGTAAT 
CAGTTTCAAA GTTTAGCGTC TTCTATACTT TCTTCCGGTA ATTTGGAGAA CTCTGATGAC 
GAAGATGTTT CTTTAGGTCC ACAGACATAC CGTTTCAATA GTTCCTTTCA TCCTTTTCGT 
TCTGCATTTT CTGCTCCAGG TGTTCAATTT GGACGCTTGT TAGAAGGAAT AAAGGATTTT 
AGTGATCCTA CTGTTCAAAT GCTTTCTCTT CAAGAACTTT CCGAAGCGTT TGCTATGAGT 
ACTGAAGATA TGCTTGTTGG ATTGTTTTCA ACCGATTCAT ACATTGCGGC TTTTTCCGAA 
ATCTTATCTG GGCGAAATTA TGATTTCGGA GAAGTCTCTA TTCAACTTAT GCTATCCTGT 
ACTACCTGTG TATCTAATAT GATGGAAGCT TTGCCTTTAT GTATGGCTAA AATAGCATAT 
AGTCCTATTG TTCGCATTCT GTGTGAAAGA ATGTTTGATA TGCAATACAT TGATATTGCT 
GAACAAGCTT TAGGAGTTTT AGAACGGCTT TCTAAAGATT TTGGTATTTG TATTTTGGAA 
CACAGAGGAA TGTTAGCTGC TCTCCAATAC TTTGATTTTT TTTATACTAC AGTTCAACGG 
ACAGCTATTT CTCTGGCTGC TAATTGCTGT AAGTTTTTGG ATGAATCCAA TGCTTCCGCA 
GCAGAAGAAA TTATTCCATT GCTTTCTAAT ATTCTACAAT CTTCTGATAC CATAGTCGTT 
AGTAAGGCAT ATTCTTGCCT GGAGACAATT ATTGAATCTT TGAAAACTTC ACCAAACATA 
ATTGAAACGA TAATTTCCGA GGATTTGATC ACCACTATTG TCAATGCTTT GACTAATTCT 
ACCTCACAAA ATAAGTCAAT GCACTTACAA GTGCAGCTAT TACATATAAT ATCATCTTTG 
TGTCAGTCAT CTTCTGCTTT GATTTTACCT TTTCTTAACC ATAATTTGCC GGATGTGATG 
TACGAAATGC TGTGTGGAAT ACCACCTTCT GATACATCCC ATCAGGCTGA TATGATTACA 
ATGCAAAGTT TGTATTATTG TCCGATCGAG TTAATAGAAA ACTTGTTAAG AGCTATAACT 
TCTTTGCTTC CTAAAAACAC TAGTAACTTG TCTGATGAAT TTAACACGAA ACTATATCGA 
TTAAACAGTA TACTCTTAAC TGTTGTAATG GACATTTATT TTATTGTGCC CCTTCATGAT 
ATCCGATCGT TAGCTGTGAC TACGGCATTG AAAATGTTAT GTTCAATTAG GGAAAATAAT 
TTAGATGGCT TAGTTTGTTC TCTTCCTTTA TCATCATTCA TTGCTTCAAT TTTAAATTCA 
CGCAAATCTG ACAATTTCTT GAAGCGAGAT GCTTTAGAAA TGTGTCTATT TTTGCTTGAA 
GCACTTCCTA ATGTTTATTC TTCGTTATTT ATACGAGAAG GCGTTGTACA GGAGGTTGGA 
TTTTTAGTTC GCTCTACCAA TGCAGACATG AAGAAAATAA AGTTAAGTAT TTCCTTTTCA 
CAAAACAAAT CTGCTGCTCG TCATGAGGAG TTGAAGAATT TATCGACGTT AAAGAGCCTT 
GCCAAGGAGT TTCTCTCTAA TTATAAGGAA GAAAATTTAG AAAACTCAAC ATTAGTTCAA 
CTTAAACAAT TGTCTAAACA TTTATTAAGT GAGACCAAAC AAGACGAATC TTTTTCAGAA 
CTAGCCAAAA TATTTCAAGA AGGATCTAAT ATTACTAGTC ATGAATTACT TCATTCTGGA 
TTAATTCATA ATTTGCTTTT ATCACTCAAA AAATTTGGAA GTTCATCTCT TCGAACATTT 
TTGTTAGCTA TGAATACATG TGATGAACGC GAAGTTTTAG AATTTGGTAA TGGTCCCTTA 
GTTTCACTTA TCTTTTGTTT GCAAAATCTA CTTTCAACTG TTGAGAATTT TCAACTTTCA 
ACGTTACCCC CAGATACTGA GAATGCGGTA GATCATGTTT TTTCGCGGCA GTTTAAACTT 
CGTTTAATGG CTCTTCCGGG ATCAAGAATT CGACCACCTT TCCGATCTCT TGTCGTTTCT 
ATTAATGGGC TAGCTACTAT TCGTACACTT GATAATTATC TTCACAGTCG CATTTCTGTG 
CGTAATGAAA CTGGACGTCG TTTTTCAATC CTCCGCGAGG CCGGTTCTTT GAGGGAGTCA 
ATGAGTGGAT CATCTCGTAA CTCTTCAGGA GACTATACTG ACTCAATGTC TCAAGATGCT 
CCTAATCACA CAACAGAGCC ATCAGAGCGA CGTGACTCCT CGACATCATC ACATTTCGAG 
GAACATTTTG TATTCAGTTT GATGGGTAAA AAGGTACCCC GAAACAAAAC AATATTCAGA 
ATTTTATATG AGTATATACA GTTGTCTGAC GATCATACTC TCGATGATTT TTGGAAAACT 
CCTGTCCCAA TATTTTATGG TGAGCCTGAT TGCATTCACA ATGATATGAA AGGGGAGTTA 
AACTATGAGA ATGAAACAGA GGGATTTTCT ATAAATATTA GAGAAATATT GGATTTATTG 
AGTATTTTGT ATTATGGGAT TAGAGACGTT CATACCCTCT TTCCTGACAA ACATTTCCGT 
GGCAACATTG AAAACATTCT TACAGATTTT AGCAACTGGA AGTTGAGTGC CAAATTGAAT 
AGACAACTTG AAGAGCAACA ACTTGTTGTT CACGGTTGTT TACCCTCCTG GTGTATATCA 
CTAACCTCTG CATATCCATT CCTAGTACCG TTTGAAACTC GATATCTTTT ATTGCAATCT 
ACTGCTTTTG GTTTATCAAG ATCCGTTTCT TTTCTTTTGT CCCGAAATCC TGAGCTATCA 
AAAAACGAAA GTTCCTCCAT ACTTCAATTT GTCAGCTTAC ATCGCCAGAA AATTCGTATT 
TCTCGGAAAA AAATATTTAA TTACGCTTTG CATTTGCTTG CAACTTATGC GGCTTCTGAA 
AATATTCTTG AAATAGAATA CGAAGATGAA GTTGGTTCAG GTCTTGGGCC CACTTTGGAG 
TTCTATACTT CAGTATCTAA AGAATTTACC TTAAATTCTC TCGATATTTG GCGGAATGAT 
CAACCCAACT CTAAATTTGT GTACCAAGCG TCTGGACTTT TTCCTTCTCC TATTCCATTA 
TTAGGGTCCA GTCCAGAAAA TGAAAGAAAA ATTTCGTTGT TCTTTGCTCT TGGACAGTTC 
GTTGCACGCT CGATTTATGA TTCAAGGATA ATAAGCATTC AGTTTAATCC ACTTTTCTTT 
GCACGAAATA TACCTTTAAC CATTTCCTCT GTAGCGAAAG TTGACAAGGG CTTAGCTAAC 
TCGTTACGAT ATTTAGAGAA ATTAATACCG GGTAAAAACC CTACAAACGC CGAGACGGAC 
ATTAAGCTTG AAGACCTTCA TCTCGATTTT ACTCTACCGG GATTTCCGTC TATTGAACTC 
ATACCTGATG GAGCTAGTAC ACCTGTGACA ACATTCAATG TGAGTGATTA TTTAAATTAT 
GTCATTGACT ATACTGTTGG AAAGGGTGTT CAACAACAAT TAGAAGCATT TCAAAATGGG 
TTTTCCAGCG TTTTTCCTTA TACTTCTTTG CAAGTTTTGA CAGAACATGA ATTGGTCACA 
CTATTCGGTA CTGTCGATGA GGATTGGAGT TACGCGACCT TGATGAAATC TATAGTAGCA 
GATCACGGTT ACACCATGGA AAGTCCTACA ATCCAGAGAT TACTGACTTT GATGAGTCAA 
ATGAATTTTC AAGAACAAAG AGACTTTTTG CAATTTATTA CTGGAAGCCG AAAACTTCCT 
ATTGGAGGAT TTGCTGGCCT AAATCCCCCG TTAACTGTTG TTAGGCGCTT AAATGAACCT 
CCTTATGTAC CTGATGATTA TCTTCCAAGC GTGATGACTT GTGTGAATTA TTTGAAACTA 
CCTGAGTATT CTTCAAGTGA GGTTTTAGGA AGTCGACTTT CTAAGGCTAT TTTAGAAGGA 
CAGGGCAGTT TTCATTTATC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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SPAC12B10.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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