SPBC83.15 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPBC83.15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC83.15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101175 NM_001021541.2
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Schizosaccharomyces pombe putative ribosome biogenesis protein Nsa1 (SPBC83.15), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc101175
    Clone ID Related Accession (Same CDS sequence) NM_001021541.2
    Accession Version NM_001021541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ribosome biogenesis protein Nsa1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021541.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAATTGC TTCTTGGTGA TGAAATCGGG CAATTAAAAT TCATAGAAAT AAAAAAAGGT 
    ACTGACACCT CAAACCCCGA GTCAGAAGCA CCAGTTATTC AGAAATTTGG AGAGTTAGAT
    CGAGAAAAAG GAGTGCTTTT TATGTTGAAG CATGAAATGA ATGTATTTGT CGCTCGGAAA
    AATGGTACTA TTGAATGTTG GAACGTTAAC CAAGAACCAC CTATTTTGTC TAGTCTTTGG
    CAATTGGACA GCTCTTTATT AGAGACTGCT TCCATAGTTT CCATGAAATA CTCTAATGGT
    TGGCTAATGC TTGCTTTAAG TGATGGAAAT TTGTTGTTTA GACACATTGA ATCTTCGAAG
    CTTCGAAAAC TTCAATTACA TGGCCCCCTG TCCGCTGTTG AGTTACATCC AAGAATACCA
    GGAATAATCG CTGCTGGAGG AAAAGAAAAC GATGTTTGTT TGTATTCTTG CAATCCTACG
    TGCAAATCGA ACATTGATGA ACTAGAACTT TGGCGAACAG AAAACGTGGT AAAAGTTTTC
    CAGGGAAAAA ATGTAAAAAA TGACTCCCTG AATCTTAGAG TGCGCGTGTG GATCACTGGA
    ATTGTGTTCA CGGAAGATAT TATTAATGTT ATTGACGGAA AGTCCGAAGA CGATGAAAGT
    CTGTGCTTCC ATTTTGCTAC GATTACTCAT TATGGTCAGT TACGCTTTTA TGATACGAAA
    CACGGCCGCC GCCCGGTTTC CACATTTGAT GTCTCTACTT CACCATTAAG TCATGTTGGT
    TTGCTTCCTT CAATCAAGTT ACTTTACTTT GCTGATAAGC GTGCTCAAAT CTCAATTTTT
    GACCATTCAA AGAAAAAAGT CATCGGAAGA TTTCAAGGAG TAAAGGGTGC CCCTTCTTCA
    ATTCATTGCC TTGGTAATGT AGTTGCTATT ACTGGTTTAG ATAGAAACGT CAGGATTTTT
    GATGCTGATA GAAAACCGCT AGCAAATGCA TATATTAAAG CTCTTCCTAC CAGTATTATT
    GTTATTAATG AAAGAGATGC CGAAATAATT AAGAAAGAAG AGGAATTAGA AGCGGCAAAA
    GAAGAAGAGG AAGAGATTTG GCGAAACATG GAACAGTTAG AAGATACAGA AGATAAAAAG
    CCTTCGAAGA GGATAAAACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595647.1
    CDS97..1260
    Translation

    Target ORF information:

    RefSeq Version NM_001021541.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ribosome biogenesis protein Nsa1 (SPBC83.15), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAATTGC TTCTTGGTGA TGAAATCGGG CAATTAAAAT TCATAGAAAT AAAAAAAGGT 
    ACTGACACCT CAAACCCCGA GTCAGAAGCA CCAGTTATTC AGAAATTTGG AGAGTTAGAT
    CGAGAAAAAG GAGTGCTTTT TATGTTGAAG CATGAAATGA ATGTATTTGT CGCTCGGAAA
    AATGGTACTA TTGAATGTTG GAACGTTAAC CAAGAACCAC CTATTTTGTC TAGTCTTTGG
    CAATTGGACA GCTCTTTATT AGAGACTGCT TCCATAGTTT CCATGAAATA CTCTAATGGT
    TGGCTAATGC TTGCTTTAAG TGATGGAAAT TTGTTGTTTA GACACATTGA ATCTTCGAAG
    CTTCGAAAAC TTCAATTACA TGGCCCCCTG TCCGCTGTTG AGTTACATCC AAGAATACCA
    GGAATAATCG CTGCTGGAGG AAAAGAAAAC GATGTTTGTT TGTATTCTTG CAATCCTACG
    TGCAAATCGA ACATTGATGA ACTAGAACTT TGGCGAACAG AAAACGTGGT AAAAGTTTTC
    CAGGGAAAAA ATGTAAAAAA TGACTCCCTG AATCTTAGAG TGCGCGTGTG GATCACTGGA
    ATTGTGTTCA CGGAAGATAT TATTAATGTT ATTGACGGAA AGTCCGAAGA CGATGAAAGT
    CTGTGCTTCC ATTTTGCTAC GATTACTCAT TATGGTCAGT TACGCTTTTA TGATACGAAA
    CACGGCCGCC GCCCGGTTTC CACATTTGAT GTCTCTACTT CACCATTAAG TCATGTTGGT
    TTGCTTCCTT CAATCAAGTT ACTTTACTTT GCTGATAAGC GTGCTCAAAT CTCAATTTTT
    GACCATTCAA AGAAAAAAGT CATCGGAAGA TTTCAAGGAG TAAAGGGTGC CCCTTCTTCA
    ATTCATTGCC TTGGTAATGT AGTTGCTATT ACTGGTTTAG ATAGAAACGT CAGGATTTTT
    GATGCTGATA GAAAACCGCT AGCAAATGCA TATATTAAAG CTCTTCCTAC CAGTATTATT
    GTTATTAATG AAAGAGATGC CGAAATAATT AAGAAAGAAG AGGAATTAGA AGCGGCAAAA
    GAAGAAGAGG AAGAGATTTG GCGAAACATG GAACAGTTAG AAGATACAGA AGATAAAAAG
    CCTTCGAAGA GGATAAAACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L