def1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following def1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the def1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100430 NM_001021140.2
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Schizosaccharomyces pombe putative RNAPII degradation factor Def1 (def1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100430
    Clone ID Related Accession (Same CDS sequence) NM_001021140.2
    Accession Version NM_001021140.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative RNAPII degradation factor Def1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021140.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTGAGA ATAAAAAGTT TCGGCCAAAG AAGGTCGGAA ATACCGAACA AGTTTCAGAA 
    AAGCAACACA CAGATACAGC GTTAAATATA TCGCAAGATA ATAAAACTTC ATCCTCTTCC
    GCCAAGAGAG GAGCGAAGGG AAGGCGAGGC ACCAAAGGAA AATCTGGCCC GGCAAATTCG
    TCTCAGACTA GTAATATAGA GGGATCAAAT GCGCTTGAGC TCGAATTTGA TCGTGATTCG
    CAGTTAAGCG TATTGCAAGA GCTGTTTCCT AGCTGGACGA TTGATGATTT AAGTTTTGCT
    TTAGAAGAAG CGGACCGGGA TTTGGAACTG ACCATATTGC ATATTACTGA AGGTCACGCC
    TCCAAATGGG GTGAAGTTAA AAAGAAGCCA ATCCCTAAAA CAAAGTCCAA ACCAACAAGT
    CATGCTCCAG TTTCCGATAA TGTCTCTTCC ACCTTTCGTA ATGCTACACG TAAATCAAAG
    AAACCTTCCG CTTCTAAGGA TACTTCTCGG GGGGTTCGAA AATCAAAAGC AGGAGCTCCC
    TCAGATCCTT CTTCCGTTCA TGCACCATCC TCTTTAGAAA AGCCTGCTGG TACTGGCGAT
    CTTCCTTCCT CAGAAATTTC TACCAAAGCT CCTGCATCAA CGACAGTGTC TTCTTCCGTT
    GATCCTGGTA CTATTAATGA AGATTCCTCA ATGAAAGATC ATACTACATC TAATGAACAA
    GCTTCAGTTT TAACATCGGC TAATACCGCT GCTTCAACAA ATACGAACGG CACCGCAGGC
    GGTACTTCGG CCTCAGCAAA GTCGACCAGT GCGGCTGATC AGGCTGTTGC ATCTTCAAAG
    CCTATAAAGA AGGCTTGGGC TTCAGTTGCA AAGTCGAAGA AAAAGGTCAC TCCCGCTCCC
    GCTCCCGCTC CCGAATCCGA ACCTTCTAAA CCCTCTATCG CTCCTTCTCA ACCCTCTAAA
    ACTAATGTTT CCGCTGCTTA CGAAAAACCT GCTGAACTGT CATCCTCATC TGTTCCTTTC
    CCTCACAAAT CCCAGGACTC TGCAACTCCC GCAAATGTTG AAACGACCCC CTCCACAGCT
    ACCTCAGCTC CCAAAAAGAG TACTGCCCCA TTTGCCATAA ATGCAGTCAA GCCCGCTCCT
    GGGCTTTCCA ACATCTCTTC TGCTTCCCTA CCTAAGCCCT CCTTTGCGAA GCAAGCCGCT
    GTTGGCTCTC AAAGCTCTAC AACTAGTATG ACTACTGCTC GATTACTTTC TGATAGATTC
    CCCGTCGTTA TGCCTGTTGC TAATACTGCT GCTATTCCCG AAAAGGTTCA AGTGCGTTTC
    GGAAGCTTGA CACTTGGGGG TGAGGACAAA AAATCGACAA AGTCTTCTTC AGACAACATA
    GCACAGTCTG GACCAAGAAG TTCCTATTTT CCTAAAAAGA CAGTTTCACC AAAGCCAGAG
    GCAAAAAAAG AGGCGAGCAA AGTTGCTGAA TCCACCAAAA TCCCAAAGAA ACAACACACT
    TCAGCATATG AAAGCCGTGC TCCACAATCG AAGGTTCCCG AAAACTTGAA GGAATCTCAT
    GTTAACGAGA CACCATATCG TGGTTTACAC GATGTAAATT TACCGGCCAG TGGAAACGCC
    TCTTCTGTCT CGGCCATCCC TCCTCAAGTA TCAGCCCAAA CGACTATACC CGCTGCATCC
    ACAAGTGCTA CAAGTGGCCC GGTGGTTGGT AAACAAAGTG CCGGATATGG CGGTATTTAT
    TCTCCTGCTA CACATGGAAT TTCCCCTCAA CCATATGCTG GTGCTAGTCT TATTCCTCCG
    TCTACCGGAG CTGCTTTTAA CAACGAAACT GCATCTTCAA ATGCTGGGGA GACAACTGCA
    CTTCCATATT CTTCTAGATT TATGAATCCT GTGGAAAACC GCTCCGCCAA TAGCCCATTC
    GACAATTTTC AACACATCGA CCATAGTACT GAATTGGGAT ATGCTTCAAA TACTTCTCAA
    CAATATCAAC AGACTAGTCA TCCAGCATAC ACTACTATGT CTCCTATTGA ATATGGTCAA
    GCTGCACCAG CAGCTCATTA CTATAATGAG CGTAATCAAT ACTCACAATA TGATAATGTC
    GCTGGTAGTG CGTCGAATAC ACCAGATGTG CACTCGGTAA TGCATAACAA GGTTGCATCT
    TCTAATGCTA CTTCTCTACC TGCTGCTGGA ACAGCGACTC CTAGCCCTGT TGTTTCTCAA
    CAACAGCCCC AACCTTATGC ATTTCCTCCA ATGTATCCTA TTCCTTATGT GTCTTATGGA
    TACGGTACGA TGCCTTACAA TAATAACAAG TTTGGACAGC CCCAGCAAGG ATACATGTCT
    CAGAGCGGCT TTAATGATTT CCCTCCCATC TTTGGTGGAC ACAGCAACGT GTACAACAGA
    CAACAGCCTG GCAATGTAAG TGGTATGAGT GGAACACAAA CTAGCAACCC AATTAATAAT
    GCCACTGCAA ATACTAGCGG CATGACTGAG AAAGCGGCTG GACATCGTGA CTCTGTGATT
    GGAAATGGAG GCGTAACCGG GGCGAACAGC ATGTCAAGTA CTTTGGGTGG TTTGTCTTCC
    ATGTCAGGGA TGGGACGTGC GGCTCCTGGT ATGTTTATGG GTAATGTTGG AAGTTTTGAA
    AACCTTAGTG CCAATGCGGC CTCTCCTTAT GGCTTACCTC CTCAACAAAC GCCATTGACC
    AATGCTGCTA CTCAGCAGAC AACCTCTTTT CAAAGCAATG CTAACAAGGT CAACAATGCA
    AACAGCCAAC CCGCTGGGTT TCCGTTCAAC TCTGGAGCAT CTACCAATGC AGGTAGTAAT
    GGATTAGGAG GCTATAATGC ATACAACCAG TCATTTATAA ACCGACCAGG AGGTTGGTAC
    GGTAATGCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595234.1
    CDS157..3048
    Translation

    Target ORF information:

    RefSeq Version NM_001021140.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative RNAPII degradation factor Def1 (def1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021140.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTGAGA ATAAAAAGTT TCGGCCAAAG AAGGTCGGAA ATACCGAACA AGTTTCAGAA 
    AAGCAACACA CAGATACAGC GTTAAATATA TCGCAAGATA ATAAAACTTC ATCCTCTTCC
    GCCAAGAGAG GAGCGAAGGG AAGGCGAGGC ACCAAAGGAA AATCTGGCCC GGCAAATTCG
    TCTCAGACTA GTAATATAGA GGGATCAAAT GCGCTTGAGC TCGAATTTGA TCGTGATTCG
    CAGTTAAGCG TATTGCAAGA GCTGTTTCCT AGCTGGACGA TTGATGATTT AAGTTTTGCT
    TTAGAAGAAG CGGACCGGGA TTTGGAACTG ACCATATTGC ATATTACTGA AGGTCACGCC
    TCCAAATGGG GTGAAGTTAA AAAGAAGCCA ATCCCTAAAA CAAAGTCCAA ACCAACAAGT
    CATGCTCCAG TTTCCGATAA TGTCTCTTCC ACCTTTCGTA ATGCTACACG TAAATCAAAG
    AAACCTTCCG CTTCTAAGGA TACTTCTCGG GGGGTTCGAA AATCAAAAGC AGGAGCTCCC
    TCAGATCCTT CTTCCGTTCA TGCACCATCC TCTTTAGAAA AGCCTGCTGG TACTGGCGAT
    CTTCCTTCCT CAGAAATTTC TACCAAAGCT CCTGCATCAA CGACAGTGTC TTCTTCCGTT
    GATCCTGGTA CTATTAATGA AGATTCCTCA ATGAAAGATC ATACTACATC TAATGAACAA
    GCTTCAGTTT TAACATCGGC TAATACCGCT GCTTCAACAA ATACGAACGG CACCGCAGGC
    GGTACTTCGG CCTCAGCAAA GTCGACCAGT GCGGCTGATC AGGCTGTTGC ATCTTCAAAG
    CCTATAAAGA AGGCTTGGGC TTCAGTTGCA AAGTCGAAGA AAAAGGTCAC TCCCGCTCCC
    GCTCCCGCTC CCGAATCCGA ACCTTCTAAA CCCTCTATCG CTCCTTCTCA ACCCTCTAAA
    ACTAATGTTT CCGCTGCTTA CGAAAAACCT GCTGAACTGT CATCCTCATC TGTTCCTTTC
    CCTCACAAAT CCCAGGACTC TGCAACTCCC GCAAATGTTG AAACGACCCC CTCCACAGCT
    ACCTCAGCTC CCAAAAAGAG TACTGCCCCA TTTGCCATAA ATGCAGTCAA GCCCGCTCCT
    GGGCTTTCCA ACATCTCTTC TGCTTCCCTA CCTAAGCCCT CCTTTGCGAA GCAAGCCGCT
    GTTGGCTCTC AAAGCTCTAC AACTAGTATG ACTACTGCTC GATTACTTTC TGATAGATTC
    CCCGTCGTTA TGCCTGTTGC TAATACTGCT GCTATTCCCG AAAAGGTTCA AGTGCGTTTC
    GGAAGCTTGA CACTTGGGGG TGAGGACAAA AAATCGACAA AGTCTTCTTC AGACAACATA
    GCACAGTCTG GACCAAGAAG TTCCTATTTT CCTAAAAAGA CAGTTTCACC AAAGCCAGAG
    GCAAAAAAAG AGGCGAGCAA AGTTGCTGAA TCCACCAAAA TCCCAAAGAA ACAACACACT
    TCAGCATATG AAAGCCGTGC TCCACAATCG AAGGTTCCCG AAAACTTGAA GGAATCTCAT
    GTTAACGAGA CACCATATCG TGGTTTACAC GATGTAAATT TACCGGCCAG TGGAAACGCC
    TCTTCTGTCT CGGCCATCCC TCCTCAAGTA TCAGCCCAAA CGACTATACC CGCTGCATCC
    ACAAGTGCTA CAAGTGGCCC GGTGGTTGGT AAACAAAGTG CCGGATATGG CGGTATTTAT
    TCTCCTGCTA CACATGGAAT TTCCCCTCAA CCATATGCTG GTGCTAGTCT TATTCCTCCG
    TCTACCGGAG CTGCTTTTAA CAACGAAACT GCATCTTCAA ATGCTGGGGA GACAACTGCA
    CTTCCATATT CTTCTAGATT TATGAATCCT GTGGAAAACC GCTCCGCCAA TAGCCCATTC
    GACAATTTTC AACACATCGA CCATAGTACT GAATTGGGAT ATGCTTCAAA TACTTCTCAA
    CAATATCAAC AGACTAGTCA TCCAGCATAC ACTACTATGT CTCCTATTGA ATATGGTCAA
    GCTGCACCAG CAGCTCATTA CTATAATGAG CGTAATCAAT ACTCACAATA TGATAATGTC
    GCTGGTAGTG CGTCGAATAC ACCAGATGTG CACTCGGTAA TGCATAACAA GGTTGCATCT
    TCTAATGCTA CTTCTCTACC TGCTGCTGGA ACAGCGACTC CTAGCCCTGT TGTTTCTCAA
    CAACAGCCCC AACCTTATGC ATTTCCTCCA ATGTATCCTA TTCCTTATGT GTCTTATGGA
    TACGGTACGA TGCCTTACAA TAATAACAAG TTTGGACAGC CCCAGCAAGG ATACATGTCT
    CAGAGCGGCT TTAATGATTT CCCTCCCATC TTTGGTGGAC ACAGCAACGT GTACAACAGA
    CAACAGCCTG GCAATGTAAG TGGTATGAGT GGAACACAAA CTAGCAACCC AATTAATAAT
    GCCACTGCAA ATACTAGCGG CATGACTGAG AAAGCGGCTG GACATCGTGA CTCTGTGATT
    GGAAATGGAG GCGTAACCGG GGCGAACAGC ATGTCAAGTA CTTTGGGTGG TTTGTCTTCC
    ATGTCAGGGA TGGGACGTGC GGCTCCTGGT ATGTTTATGG GTAATGTTGG AAGTTTTGAA
    AACCTTAGTG CCAATGCGGC CTCTCCTTAT GGCTTACCTC CTCAACAAAC GCCATTGACC
    AATGCTGCTA CTCAGCAGAC AACCTCTTTT CAAAGCAATG CTAACAAGGT CAACAATGCA
    AACAGCCAAC CCGCTGGGTT TCCGTTCAAC TCTGGAGCAT CTACCAATGC AGGTAGTAAT
    GGATTAGGAG GCTATAATGC ATACAACCAG TCATTTATAA ACCGACCAGG AGGTTGGTAC
    GGTAATGCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L