cyr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cyr1
Entrez Gene ID	2540810
Full Name	adenylate cyclase
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022079.2	NP_596159.1	adenylate cyclase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gpa2 exhibits a direct and GTP-dependent binding to the Ras-associating domain (RAD) of cyr1
Title: Direct activation of fission yeast adenylyl cyclase by heterotrimeric G protein gpa2.

The following cyr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cyr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100682	NM_001022079.2 Latest version!	Schizosaccharomyces pombe adenylate cyclase (cyr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cyr1 ( NM_001022079.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596159.1 Schizosaccharomyces pombe adenylate cyclase (cyr1), mRNA.

Price & Availability↑

CloneID	OSc100682
Clone ID Related Accession (Same CDS sequence)	NM_001022079.2
Accession Version	NM_001022079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	adenylate cyclase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022079.1.

ATGGATCAAA GCAAGCGATT GTTAAAGTCA GCTGTTCCAA ACCCGCCTGA ACATTTTAAA 
ACAGGTATAT CCTGGCTTGA TGACCTTGAC GAGAAGGACG ATGACTCAGC GACGTCTGTG 
AACTATGATA TACCAGAAAT AACCGAAGCA AATCTCTGTA ATGACTCCCA TGAAGCTCTT 
TCACCTTGCA CTCAACCTGT CGGTAATTCT GGACGACCTG TCGAAGCTTT TAAGACCTAT 
CCTAGCACTC CTGCAGTACC TAGCAAAAGC GTGCTTTTCC ATTTTTATGA ACCGGATGAG 
AACTTTAGCT TATCTGACAC GGGACGTACT AAATCGGATA CCGCCTTAGC TGCAAGAGAA 
AGCTCTGAAA AGTCTGAAGT GCCACGTGAT ACCCGTAGTG CTGGGATAAA GCCATACAAA 
GAAAATAACT CGTCTAATTG TGCAATTTCT AAAGAAGCAG GCCTTCGAAG ACTTATTGAT 
AAGGACAGAG AATCTTTCGA CAAAAACCTG AATCAGTCAT TTACCAATCT AACTTTTCCA 
GAACCGATTT CTGATGACAG TGACAGTGTG GAGTTTCAAC GTGATTCTCT TAATAACAAT 
TGGCCAGCTA GTTTGGAAGG TAGCATACAC GAATTACCCA GGAATAGTGA TGATGATGGA 
ATCCCTGCTT CTGCGGCTCA TATCCTGGAC CTCGATTATC ATAGAGATAG TTATGATAGC 
CCTTGGAAGA AGTTTTTACC GTACCCTTCC ATTTTATCCG ATGATTCTTG GAAAGCTCCG 
GAGAGTTGGG GAACTAGTTT GCCCACTGAG GCTATTCCAA AGCAGGTTTT TACTACTAGA 
TTTTTTGCTC GTCCTTCCCT GGGCAATAGA AAGAAAGAGT TCTTTCTTCG AGTATACCGT 
GATGATCGAA CTTCCGTATC ATTCATTTGC CCTATTGGCA TACAAACTCA TGAAGTGATT 
AAGCTGTTAG CAAGGTTATT TTTTCTTCCC TCGTCTGCTA ATTTTTACCT CTTATTAATC 
CAATTCAATA CAGAACGTAT TTTGTTGCCT CATGAACAGC CATGCATTAT ATTTGAACGA 
TTATTGAGTT TGTTTGGGTG TAAGGTAACT TCCGATGAAG AAATAAATGA AGAAGATAAT 
TATAGTGTTG CTAGATTGGT GTTTACTACG ATGGATATTG GAGCGGATGT ATTGCGTAAA 
TTCTCTGAAA AAAAAATTAC TGCGAACCTT GACATTAGTA GGTCAAACCT GGAGGTTATT 
CCGGTAAAGA TCTATCCCTA TGCCCATGAA CTTATCTCGT TGAATGTTTC GCACAATTTA 
TCGCTGGACC TGCCTTTAGA TTTCATGGAG CGCTGTGTCA AGCTTAAGCG GTTAGACATT 
TCCAATAATT TAAGGTCTCC AAGAGGAAAA CCGATTACTG CTCTTCGCCA ATTGGAAGTT 
TTGAACATGT CTCGTAACGA TATATATGAA TTGGATCCTC TTATATTCTC TGGGCTTAGT 
CGAAATTCTC TGAAAGAATT GAATATAGCT AATAATAAGC TGTTCTTCCT TCCCCATTCT 
ACTAGATATT TGGTGAATCT AACCTATCTT GATTTATCCT ACAATAATTT TGTGACTTTC 
CCTTTAATAA TTACTGAGTT GTCCCAATTG GAGACTCTTA ACTTTTCGCA TAATTTATTG 
TCACAGATAT CTAGCAAAAT TGGCTCTCTT GTTAAATTGA AGCATCTATA TCTACAATTT 
AATGATTTAT CTAATCGGCT TCCACAGGAA ATAGGCTTGC TAAAAAATCT GGAAACAATT 
GACCTTAGTT ATAATGCGAT TACTAACATC GCCAGTTTAT CTGAATGTCC GAAACTAAAT 
AGCATCAATG TAGCTTGCAA TTTACTTTCT TTTTACGAAT ATTCCAATCC GTCAGCTACA 
TTCATCGACT TCTCGTTTTG TCCACTGACT ACAATTGATC CGGCATTTAG CTATAGCAAT 
CTCGTCTACT TTGATATCTC ACATGCGAAG CTTATTGGGC TCAAGGATTC TGTCATTGAA 
ACTTTAGTAA ATGTAGAAAC AGTGAAAGTG AATTACAACC ACTTTACTAG CATTTCCGAT 
GCAATTTCTG CTATGCAAAA TTTGAAATAT TTGTCTTGCA CGAACTGTGA AATGTCTTAT 
GTTTCACCAA ACCTTGGCAA ATTAAAGCAT TTAGTTCACC TGGATTTACA TGCAAATAAT 
ATCAAAATAT TCCCTGAGGA AGTATGGCAA GTCTCTTCAC TAAAAGTTGT TAACCTGTCT 
TCCAATATCC TGGAGAAAAT CAAGTTACCA GTTGCAACGT CAAAAAAATT AACTAGGACA 
ATTAGCCAAT TAAAAATCAT GCGTACTTTA TCAGGAAATC CGGTATCGAG CCTCTCCTCC 
CAAGAATTTG TTATGCCTAC CGTTGAAGAA TTATACTTGG TGGACAACAG ATTGGGCAAT 
GACTGTTTTA CGGCTTTAGA ATACTTTAAG TGTTTAAAAG TCTTAAATTT ATCTTACAAT 
TATTTAACGG AAATTCCAAG CAAGTTTTTC CAAAATTTTT CTGATCTTAA ACACCTTTTT 
GTGTCAGGAA ATGAGCTTGC AAATCTTTCC ATTTCCAGTA CAGCGCAGGT CCTACTTGAA 
ACTTTGTACG CGAATGGAAA TCGTCTTTCC TCTTTTCCTA AAAATGAAGC TTTGTCTAAA 
AGTTTAAGAT TTTTGGACAT AAGTACCAAT AATCTGCAGA ATTTAGCAGT AGAAAAAGCT 
GAAAAGAAGA GTTTAACTAA ACTTCCTCAA TTGGAATACC TTAATCTATC TGGTAACACA 
TGGTTTCGAT TCTCTGAGCA TGAAGATACA AACTTTACAA AATCATATTT GAAGAATTTA 
AAGTTTTTGA GCATCATGGA TTTGAATACA AAATTTTCTA ATGCGCCTTC TGATGTTCTA 
AACCATTTTA TTCAACGCAA CTCTCCTCAA CCTAACATTT TGAGGTATGG AGTATGTGGA 
TACCTTTCTC GTTCTATTCC CGTCATCTCT GCATGTGAAT TAGTTGTTAA CAACTTTTTG 
CATCCTCAAT CATCTTTATA CTGTGTGCTT GACAGTGACA TCAGCGCTGG AAAAAACAAC 
CGGGTTTTAA AATTTGTGTA CGACAATTTG GCTTCATGCC TAGCGCATGA GATTAATGCA 
GCAGATTCCT CTTCTGAACA AATTTGTAAC GCTTTGAGAA GAGGATTTCT TCGTTTAAAC 
AAAAAATTAG GAAATGTCAT TCATTATGAC CTTAGGAAGT CTTCAGAAGG AGACGTGGAT 
AGTAATTACG TTACCACTAT GAATATTTCA GAAAAGGGTT ATTCTATGGA CAGTTCTTGC 
TTGGATATAG GAGTTAGCAT TATCTTAGTG TACGTCAGGG ATACAAGAGC TTTCGTTGCC 
AATGTGGGCA CATCAATGGC TATAATGTCT ACGAGAAATG ATTCGGAACC TACAACTTTA 
AGTGTCATGC ATGATGTGTA CAATAGGGAT GAAATTCGTC GCATAGTTGA TTCATGTGGT 
TTCATATCTG GTGAAATAAA ATCGACCACT ACACGTGCTA TTGGTCGTTT GTCTCAATTT 
CCTGGCGTCC AAGCAGTTCC CTATGTTAAT GTGCAGTACT TATCTGAACT AAACGAATTT 
ATCATTCTAG CAAATCAGGA ATTTTGGAGC GTATTATCAA AACGCACAGT AATTGACGTT 
GTTCGTGCCA ACAGACATTC TCCTCTATTA GCCTCTACGA AGCTACGTGA CTATGCTATT 
GCATATGGTG CAGAAAAAAA TGTTCTTGTA GTTATCGTCG AATTGAACGG ATTGTTTGAA 
GAGAATTCCC TTAATTTCAA CCAACTACGG GGGGATGAAA AAACTTTAGC AATTTCTGAA 
AAGAACGATA ATATGTCATT TGTCCAAGAT TTACCCGATG ATTCTTCTTT AGCACGAATG 
AATAGAGAAG TCTCTCCCCC GAAAGGTTGT ATTGCCATGG TTTTTACTGA TATTAAGAAT 
TCCACATTAT TGTGGGAAAG ACATCCTATT GCAATGAGAT CTGCGATCAA AACTCATAAT 
ACTATTATGC GTCGGCAACT CCGTGCAACT GGAGGCTATG AAGTAAAAAC CGAAGGAGAT 
GCGTTTATGG TTTGTTTTCA AACAGTTCCT GCTGCATTAC TTTGGTGTTT TTCAGTACAA 
TTACAGTTAC TTTCGGCAGA TTGGCCCAAC GAAATAGTTG AGTCAGTGCA AGGACGGTTG 
GTACTCGGCT CAAAAAATGA GGTGTTATAT CGAGGGCTTA GTGTTCGAAT TGGTGTCAAT 
TATGGTGTAA CCGTGAGTGA ACTAGATCCC ATCACTAGAC GTATGGACTA TTACGGGCCT 
GTAGTAAACA GAACATCTAG GGTTGTATCA GTCGCTGATG GTGGTCAAAT TGCTGTTTCT 
GCTGAGGTGG TATCTGTATT GAATCAGCTT GATTCAGAAA CAATGTCATC AGAGAAGACG 
AATGTCAACG AAATGGAAGT TCGTGCTCTT AAACAAATCG GTTATATTAT CCATAACCTT 
GGAGAATTTA AGTTAAAAGG TTTGGATACT ACTGAAATGA TTTCATTGGT TTATCCTGTG 
CAATTGCAAG GAAGACTGGA GAGATTGATA AAGAGCCGAA GTTTGGGAAC ACCCACAGCC 
CTCCCGGAAA CTCAGACTTA TACTCCCGTT CGTAGTAGAA GCAACAGCTT GCGACCCATG 
TTAGCAAGAT TGAGTGATTC AAAATCTGTC CATGGAGAGG AGGGTGGTTC TGGGAAGAGA 
TCGGTTTCAT CCTTGCGCAA CGTATCACCA TCAGAGAGTA CTGGTGGATA TGAAGGTTGT 
ATTTTTGATG ACCAACAGTA TCAATTACTT TATGAACTTT GTGAGCGTCT TGAAGACCAT 
GCCGCTATAC TGCATGGGTT TCCTGAACCA CCGCCTTGCG ATACCGGTCT GGCAGCTCCC 
GTAAACCAGG CCGAGGAGTA TTCATTGTTC TACCGTCTGA CTTTGCGTAT CGAGAATACT 
ATTTATTGTG TCAGTCAAAT GCTTGGACAC ACTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596159.1
CDS	273..5351
Translation	MDQSKRLLKSAVPNPPEHFKTGISWLDDLDEKDDDSATSVNYDIPEITEANLCNDSHEALSPCTQPVGNSGRPVEAFKTYPSTPAVPSKSVLFHFYEPDENFSLSDTGRTKSDTALAARESSEKSEVPRDTRSAGIKPYKENNSSNCAISKEAGLRRLIDKDRESFDKNLNQSFTNLTFPEPISDDSDSVEFQRDSLNNNWPASLEGSIHELPRNSDDDGIPASAAHILDLDYHRDSYDSPWKKFLPYPSILSDDSWKAPESWGTSLPTEAIPKQVFTTRFFARPSLGNRKKEFFLRVYRDDRTSVSFICPIGIQTHEVIKLLARLFFLPSSANFYLLLIQFNTERILLPHEQPCIIFERLLSLFGCKVTSDEEINEEDNYSVARLVFTTMDIGADVLRKFSEKKITANLDISRSNLEVIPVKIYPYAHELISLNVSHNLSLDLPLDFMERCVKLKRLDISNNLRSPRGKPITALRQLEVLNMSRNDIYELDPLIFSGLSRNSLKELNIANNKLFFLPHSTRYLVNLTYLDLSYNNFVTFPLIITELSQLETLNFSHNLLSQISSKIGSLVKLKHLYLQFNDLSNRLPQEIGLLKNLETIDLSYNAITNIASLSECPKLNSINVACNLLSFYEYSNPSATFIDFSFCPLTTIDPAFSYSNLVYFDISHAKLIGLKDSVIETLVNVETVKVNYNHFTSISDAISAMQNLKYLSCTNCEMSYVSPNLGKLKHLVHLDLHANNIKIFPEEVWQVSSLKVVNLSSNILEKIKLPVATSKKLTRTISQLKIMRTLSGNPVSSLSSQEFVMPTVEELYLVDNRLGNDCFTALEYFKCLKVLNLSYNYLTEIPSKFFQNFSDLKHLFVSGNELANLSISSTAQVLLETLYANGNRLSSFPKNEALSKSLRFLDISTNNLQNLAVEKAEKKSLTKLPQLEYLNLSGNTWFRFSEHEDTNFTKSYLKNLKFLSIMDLNTKFSNAPSDVLNHFIQRNSPQPNILRYGVCGYLSRSIPVISACELVVNNFLHPQSSLYCVLDSDISAGKNNRVLKFVYDNLASCLAHEINAADSSSEQICNALRRGFLRLNKKLGNVIHYDLRKSSEGDVDSNYVTTMNISEKGYSMDSSCLDIGVSIILVYVRDTRAFVANVGTSMAIMSTRNDSEPTTLSVMHDVYNRDEIRRIVDSCGFISGEIKSTTTRAIGRLSQFPGVQAVPYVNVQYLSELNEFIILANQEFWSVLSKRTVIDVVRANRHSPLLASTKLRDYAIAYGAEKNVLVVIVELNGLFEENSLNFNQLRGDEKTLAISEKNDNMSFVQDLPDDSSLARMNREVSPPKGCIAMVFTDIKNSTLLWERHPIAMRSAIKTHNTIMRRQLRATGGYEVKTEGDAFMVCFQTVPAALLWCFSVQLQLLSADWPNEIVESVQGRLVLGSKNEVLYRGLSVRIGVNYGVTVSELDPITRRMDYYGPVVNRTSRVVSVADGGQIAVSAEVVSVLNQLDSETMSSEKTNVNEMEVRALKQIGYIIHNLGEFKLKGLDTTEMISLVYPVQLQGRLERLIKSRSLGTPTALPETQTYTPVRSRSNSLRPMLARLSDSKSVHGEEGGSGKRSVSSLRNVSPSESTGGYEGCIFDDQQYQLLYELCERLEDHAAILHGFPEPPPCDTGLAAPVNQAEEYSLFYRLTLRIENTIYCVSQMLGHTG

Target ORF information:

RefSeq Version	NM_001022079.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe adenylate cyclase (cyr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022079.2

ORF Insert Sequence:

ATGGATCAAA GCAAGCGATT GTTAAAGTCA GCTGTTCCAA ACCCGCCTGA ACATTTTAAA 
ACAGGTATAT CCTGGCTTGA TGACCTTGAC GAGAAGGACG ATGACTCAGC GACGTCTGTG 
AACTATGATA TACCAGAAAT AACCGAAGCA AATCTCTGTA ATGACTCCCA TGAAGCTCTT 
TCACCTTGCA CTCAACCTGT CGGTAATTCT GGACGACCTG TCGAAGCTTT TAAGACCTAT 
CCTAGCACTC CTGCAGTACC TAGCAAAAGC GTGCTTTTCC ATTTTTATGA ACCGGATGAG 
AACTTTAGCT TATCTGACAC GGGACGTACT AAATCGGATA CCGCCTTAGC TGCAAGAGAA 
AGCTCTGAAA AGTCTGAAGT GCCACGTGAT ACCCGTAGTG CTGGGATAAA GCCATACAAA 
GAAAATAACT CGTCTAATTG TGCAATTTCT AAAGAAGCAG GCCTTCGAAG ACTTATTGAT 
AAGGACAGAG AATCTTTCGA CAAAAACCTG AATCAGTCAT TTACCAATCT AACTTTTCCA 
GAACCGATTT CTGATGACAG TGACAGTGTG GAGTTTCAAC GTGATTCTCT TAATAACAAT 
TGGCCAGCTA GTTTGGAAGG TAGCATACAC GAATTACCCA GGAATAGTGA TGATGATGGA 
ATCCCTGCTT CTGCGGCTCA TATCCTGGAC CTCGATTATC ATAGAGATAG TTATGATAGC 
CCTTGGAAGA AGTTTTTACC GTACCCTTCC ATTTTATCCG ATGATTCTTG GAAAGCTCCG 
GAGAGTTGGG GAACTAGTTT GCCCACTGAG GCTATTCCAA AGCAGGTTTT TACTACTAGA 
TTTTTTGCTC GTCCTTCCCT GGGCAATAGA AAGAAAGAGT TCTTTCTTCG AGTATACCGT 
GATGATCGAA CTTCCGTATC ATTCATTTGC CCTATTGGCA TACAAACTCA TGAAGTGATT 
AAGCTGTTAG CAAGGTTATT TTTTCTTCCC TCGTCTGCTA ATTTTTACCT CTTATTAATC 
CAATTCAATA CAGAACGTAT TTTGTTGCCT CATGAACAGC CATGCATTAT ATTTGAACGA 
TTATTGAGTT TGTTTGGGTG TAAGGTAACT TCCGATGAAG AAATAAATGA AGAAGATAAT 
TATAGTGTTG CTAGATTGGT GTTTACTACG ATGGATATTG GAGCGGATGT ATTGCGTAAA 
TTCTCTGAAA AAAAAATTAC TGCGAACCTT GACATTAGTA GGTCAAACCT GGAGGTTATT 
CCGGTAAAGA TCTATCCCTA TGCCCATGAA CTTATCTCGT TGAATGTTTC GCACAATTTA 
TCGCTGGACC TGCCTTTAGA TTTCATGGAG CGCTGTGTCA AGCTTAAGCG GTTAGACATT 
TCCAATAATT TAAGGTCTCC AAGAGGAAAA CCGATTACTG CTCTTCGCCA ATTGGAAGTT 
TTGAACATGT CTCGTAACGA TATATATGAA TTGGATCCTC TTATATTCTC TGGGCTTAGT 
CGAAATTCTC TGAAAGAATT GAATATAGCT AATAATAAGC TGTTCTTCCT TCCCCATTCT 
ACTAGATATT TGGTGAATCT AACCTATCTT GATTTATCCT ACAATAATTT TGTGACTTTC 
CCTTTAATAA TTACTGAGTT GTCCCAATTG GAGACTCTTA ACTTTTCGCA TAATTTATTG 
TCACAGATAT CTAGCAAAAT TGGCTCTCTT GTTAAATTGA AGCATCTATA TCTACAATTT 
AATGATTTAT CTAATCGGCT TCCACAGGAA ATAGGCTTGC TAAAAAATCT GGAAACAATT 
GACCTTAGTT ATAATGCGAT TACTAACATC GCCAGTTTAT CTGAATGTCC GAAACTAAAT 
AGCATCAATG TAGCTTGCAA TTTACTTTCT TTTTACGAAT ATTCCAATCC GTCAGCTACA 
TTCATCGACT TCTCGTTTTG TCCACTGACT ACAATTGATC CGGCATTTAG CTATAGCAAT 
CTCGTCTACT TTGATATCTC ACATGCGAAG CTTATTGGGC TCAAGGATTC TGTCATTGAA 
ACTTTAGTAA ATGTAGAAAC AGTGAAAGTG AATTACAACC ACTTTACTAG CATTTCCGAT 
GCAATTTCTG CTATGCAAAA TTTGAAATAT TTGTCTTGCA CGAACTGTGA AATGTCTTAT 
GTTTCACCAA ACCTTGGCAA ATTAAAGCAT TTAGTTCACC TGGATTTACA TGCAAATAAT 
ATCAAAATAT TCCCTGAGGA AGTATGGCAA GTCTCTTCAC TAAAAGTTGT TAACCTGTCT 
TCCAATATCC TGGAGAAAAT CAAGTTACCA GTTGCAACGT CAAAAAAATT AACTAGGACA 
ATTAGCCAAT TAAAAATCAT GCGTACTTTA TCAGGAAATC CGGTATCGAG CCTCTCCTCC 
CAAGAATTTG TTATGCCTAC CGTTGAAGAA TTATACTTGG TGGACAACAG ATTGGGCAAT 
GACTGTTTTA CGGCTTTAGA ATACTTTAAG TGTTTAAAAG TCTTAAATTT ATCTTACAAT 
TATTTAACGG AAATTCCAAG CAAGTTTTTC CAAAATTTTT CTGATCTTAA ACACCTTTTT 
GTGTCAGGAA ATGAGCTTGC AAATCTTTCC ATTTCCAGTA CAGCGCAGGT CCTACTTGAA 
ACTTTGTACG CGAATGGAAA TCGTCTTTCC TCTTTTCCTA AAAATGAAGC TTTGTCTAAA 
AGTTTAAGAT TTTTGGACAT AAGTACCAAT AATCTGCAGA ATTTAGCAGT AGAAAAAGCT 
GAAAAGAAGA GTTTAACTAA ACTTCCTCAA TTGGAATACC TTAATCTATC TGGTAACACA 
TGGTTTCGAT TCTCTGAGCA TGAAGATACA AACTTTACAA AATCATATTT GAAGAATTTA 
AAGTTTTTGA GCATCATGGA TTTGAATACA AAATTTTCTA ATGCGCCTTC TGATGTTCTA 
AACCATTTTA TTCAACGCAA CTCTCCTCAA CCTAACATTT TGAGGTATGG AGTATGTGGA 
TACCTTTCTC GTTCTATTCC CGTCATCTCT GCATGTGAAT TAGTTGTTAA CAACTTTTTG 
CATCCTCAAT CATCTTTATA CTGTGTGCTT GACAGTGACA TCAGCGCTGG AAAAAACAAC 
CGGGTTTTAA AATTTGTGTA CGACAATTTG GCTTCATGCC TAGCGCATGA GATTAATGCA 
GCAGATTCCT CTTCTGAACA AATTTGTAAC GCTTTGAGAA GAGGATTTCT TCGTTTAAAC 
AAAAAATTAG GAAATGTCAT TCATTATGAC CTTAGGAAGT CTTCAGAAGG AGACGTGGAT 
AGTAATTACG TTACCACTAT GAATATTTCA GAAAAGGGTT ATTCTATGGA CAGTTCTTGC 
TTGGATATAG GAGTTAGCAT TATCTTAGTG TACGTCAGGG ATACAAGAGC TTTCGTTGCC 
AATGTGGGCA CATCAATGGC TATAATGTCT ACGAGAAATG ATTCGGAACC TACAACTTTA 
AGTGTCATGC ATGATGTGTA CAATAGGGAT GAAATTCGTC GCATAGTTGA TTCATGTGGT 
TTCATATCTG GTGAAATAAA ATCGACCACT ACACGTGCTA TTGGTCGTTT GTCTCAATTT 
CCTGGCGTCC AAGCAGTTCC CTATGTTAAT GTGCAGTACT TATCTGAACT AAACGAATTT 
ATCATTCTAG CAAATCAGGA ATTTTGGAGC GTATTATCAA AACGCACAGT AATTGACGTT 
GTTCGTGCCA ACAGACATTC TCCTCTATTA GCCTCTACGA AGCTACGTGA CTATGCTATT 
GCATATGGTG CAGAAAAAAA TGTTCTTGTA GTTATCGTCG AATTGAACGG ATTGTTTGAA 
GAGAATTCCC TTAATTTCAA CCAACTACGG GGGGATGAAA AAACTTTAGC AATTTCTGAA 
AAGAACGATA ATATGTCATT TGTCCAAGAT TTACCCGATG ATTCTTCTTT AGCACGAATG 
AATAGAGAAG TCTCTCCCCC GAAAGGTTGT ATTGCCATGG TTTTTACTGA TATTAAGAAT 
TCCACATTAT TGTGGGAAAG ACATCCTATT GCAATGAGAT CTGCGATCAA AACTCATAAT 
ACTATTATGC GTCGGCAACT CCGTGCAACT GGAGGCTATG AAGTAAAAAC CGAAGGAGAT 
GCGTTTATGG TTTGTTTTCA AACAGTTCCT GCTGCATTAC TTTGGTGTTT TTCAGTACAA 
TTACAGTTAC TTTCGGCAGA TTGGCCCAAC GAAATAGTTG AGTCAGTGCA AGGACGGTTG 
GTACTCGGCT CAAAAAATGA GGTGTTATAT CGAGGGCTTA GTGTTCGAAT TGGTGTCAAT 
TATGGTGTAA CCGTGAGTGA ACTAGATCCC ATCACTAGAC GTATGGACTA TTACGGGCCT 
GTAGTAAACA GAACATCTAG GGTTGTATCA GTCGCTGATG GTGGTCAAAT TGCTGTTTCT 
GCTGAGGTGG TATCTGTATT GAATCAGCTT GATTCAGAAA CAATGTCATC AGAGAAGACG 
AATGTCAACG AAATGGAAGT TCGTGCTCTT AAACAAATCG GTTATATTAT CCATAACCTT 
GGAGAATTTA AGTTAAAAGG TTTGGATACT ACTGAAATGA TTTCATTGGT TTATCCTGTG 
CAATTGCAAG GAAGACTGGA GAGATTGATA AAGAGCCGAA GTTTGGGAAC ACCCACAGCC 
CTCCCGGAAA CTCAGACTTA TACTCCCGTT CGTAGTAGAA GCAACAGCTT GCGACCCATG 
TTAGCAAGAT TGAGTGATTC AAAATCTGTC CATGGAGAGG AGGGTGGTTC TGGGAAGAGA 
TCGGTTTCAT CCTTGCGCAA CGTATCACCA TCAGAGAGTA CTGGTGGATA TGAAGGTTGT 
ATTTTTGATG ACCAACAGTA TCAATTACTT TATGAACTTT GTGAGCGTCT TGAAGACCAT 
GCCGCTATAC TGCATGGGTT TCCTGAACCA CCGCCTTGCG ATACCGGTCT GGCAGCTCCC 
GTAAACCAGG CCGAGGAGTA TTCATTGTTC TACCGTCTGA CTTTGCGTAT CGAGAATACT 
ATTTATTGTG TCAGTCAAAT GCTTGGACAC ACTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cyr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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