sre1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sre1
Entrez Gene ID	2540730
Full Name	sterol regulatory element binding protein, transcription factor Sre1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021591.2	NP_595694.1	transcription factor Sre1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sre1 and Mga2 are each regulated by the lipid product of the other transcription factor pathway, providing a source of coordination for these two branches of lipid synthesis
Title: Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.

we describe the impact of silencing and clustering on the mobility of an endogenous Tf2 element. Deletion of genes such as set1(+) or abp1(+) that both alleviate silencing and clustering, result in a corresponding increase in mobilization. Furthermore, expression of constitutively active Sre1, a transcriptional activator of Tf2 elements, also alleviates clustering and induces mobilization
Title: Restriction of Retrotransposon Mobilization in Schizosaccharomyces pombe by Transcriptional Silencing and Higher-Order Chromatin Organization.

Here we report the in vitro reconstitution of the complex between the C domains of Sre1 and Scp1 as well as the crystal structure of the WD40 domain of Scp1 at 2.1 A resolution.
Title: Structure of the WD40 domain of SCAP from fission yeast reveals the molecular basis for SREBP recognition.

We describe our isolation and characterization of the casein kinase 1 family member Hhp2 as a novel regulator of Sre1N.
Title: Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis.

Data indicate that when oxygen is present, the prolyl hydroxylase Ofd1 down-regulates sterol regulatory element-binding protein Sre1N activity in two ways: first, by inhibiting its binding to DNA, and second, by accelerating its degradation.
Title: Regulation of SREBP during hypoxia requires Ofd1-mediated control of both DNA binding and degradation.

The following sre1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sre1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101415	NM_001021591.2 Latest version!	Schizosaccharomyces pombe transcription factor Sre1 (sre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sre1 ( NM_001021591.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595694.1 Schizosaccharomyces pombe transcription factor Sre1 (sre1), mRNA.

Price & Availability↑

CloneID	OSc101415
Clone ID Related Accession (Same CDS sequence)	NM_001021591.2
Accession Version	NM_001021591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	transcription factor Sre1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021591.1.

ATGCAAAGCT CAATTCCGTC AGTTTCCGTG TCGGTCGCTT CTCCTGCAAT GGAAACTCCA 
ACAAAAGCTT CGCCTGACAG CAAATCACCC AATTCAGTGG GTGCGATTCC TTCTTCATCC 
CCTTTGGCTT CATCGACAAA GGCTTCAACT TCTACTCCCT TTGTGGAGAA TTGTTCCAAT 
CTGTTGTGTG ATTTGGCTAG CATTGTCGAG GACTCACCTC CAACCTTGAC CAACACCTCG 
TTGTCTCCCC ATTCGTTTTC CCTTTCTGAT ATGGAGTCAT CAATGAGCAA TTGGCTTAAC 
CCTTTTGCGT TCGACAATAC GATGAACTCG GCGCCTCCTC TGTTTACTTC GACCAACATG 
GGCTCCCCTA ACTCGTTAGA AAACTCTACA AATCCTCTTC TCTCTAATTG CGGCTCTCCA 
AATTCATTTC AGAATGAGAC CTTTACCGGC CCATCATTGA ATGAGTTCGA TGCCGATGAT 
AAAATTCAGC GCCAAATGAA AATCCTTCAC TCTGTTGATA CAATCGATCC ATCGACTGTT 
CAAAATTATC CTTCAGCTGA TATGTCAAAT CCAGAAGTCA AATTGAAAAC CGAAGAAATT 
ATTACACCCA TGGACACCAC TTGCAAGCCT GAACCTTCTG CCAAGAAAAT AAAGTTGTCT 
CCTTCCTCGG AAGATTCTTG CTCAATTCCT GAAACATTAC CTTTTTCGGC CCCCAAGTCT 
AGAGGCTCAT TATCTCCTTC TGAAACCCCA GATTTTGTAG CCGGTCCTGG AGGAAAACCA 
AAGAAGACAG CTCATAACAT GATTGAGAAG AGATACAGAA CCAACTTGAA CGACCGAATT 
TGTGAATTGC GTGATGCAGT TCCTTCACTT CGTGCTGCTG CTGCACTTCG TTGTGGTAAT 
TCTCTGGACG ATGAGGATTT GGGGGGTTTG ACACCCGCTC GTAAGCTTAA CAAGGGTACA 
ATATTGGCAA AAGCAACTGA GTATATCAGA CATTTGGAGG CCAAGAATAA GGAACTACAG 
AAGACTAACA AGCAGCTTTC AGATAGGCTG GCTTTCTACG AAGATCCTTC GATGGCTCCT 
CCTTCTAACG ATACTCGAGC CGTTAACTCT GTTAATGTTG TTTCCTCATC TGACTATTCT 
GTTCACCAAT CTTCTCGACC AAACCTAACC CAAAGAGCTT TTACTTCCCC AACCCTAAAT 
ACCATGGGCC GCACAGCACT AAATGGCATG GTTGGACTTG GTCTATTCAA TTATTTTGGA 
AACGATTCTT CTCAATCTGT TTATGGTTTA TTCGCTCTTC CACCTTTTCT TATGTCTCCA 
TTCACAGGTA CTGTTCTCTT TAACATGCTG AAAATTGGTG TTGTTTTACT CGGTTTATTC 
TATCTTTTGC ACGATAACTC TCTTTTCAAA GGCTTCAAGG GTGAAAAGAA ATCTAAAGTT 
AGTACCAGAT CTTCCATGTC ACCATCCTCA ATCTTATTCC GAAAAACCGT ATTCGAAAAG 
TATTGTTTAT TGGACCATTC TACTTCGACT ATTTCTCTAT TTTTTGGATT ACTGATCTTC 
ACTCTTAAAT CTGCTTATGG CTATTTGACT CATCGTTTGA GTGCTCTTTA TACCTCCTCT 
GAGAATTGGG TTTATAGTGA GCAACAACTT GCTGAAGTTC GCAACATGGA AAAGCTTTTG 
GATGCCCAAT TAATGGGTGG TGATGCTAAA GTTGACCGTT TGAGGCTCTT AATGGTATTT 
GCCAGTTCAT TTTCTCTTCC TCCTTCAAGT CATACTTGTG CTTTACAAGC AATGTATTGC 
CAACTAATTT TTTCCAACAC CTCTGTCCCA TCCGCTATCG TTTCGAAGTG CGTTGCTTTC 
TTCTGGAATG CTGCAAAGAA ACAACATAGC AAAAGTTCTG TACACGCGGA GTTACGTGAG 
CTTCCTGAGT GTACTGCTAA CCTAATTGAA AACTCCCACG CTGACGACGT TTTCTCTCCG 
AATATGGTTG AGCGATTGTG GGTTCTAGCA AAGTGCACCC GTGACTCTGC TCAAATGTCA 
GATAGTATTA TTAGCTCCTT ATCTGATGTT TTGGTTTTAT CTCCTCTAGA GGTGCTTGCC 
TCATGGTATG CCGCTGATTT GTTGGATGCC CTTTTGATGG AAAGTTTGAG CCGTAAAGTT 
GAGATATCCG AGATTGAGGA AATTATTTCC CTTTGTCCTA AGAACTCCTC TATTATTCGT 
CATGCTTTAC TAGCTAAATT GGTTTTGTTC CCCGAGAATA CAGCTGATTC TTTGAATGAA 
GTCCTTGCAG CATACAAGAA CACTTTGGAT TTATGCAGCC AAGATAAACG CAAGCAGTCA 
AGCGTGTTAA AGATCAACTT ATCCAAGCTA TTCACACTCC ACTCATGCCT ATCATTAGCA 
CTCCAACGCC TTGGATACGG GGATGTTTCT AAACGTATGT ATCAGGAAAT CTTTGTACCT 
GATAGTGATG CAGACATTAC ACCTTTATCT TTTATTATTT CCTGGACCGC TTTGAACACT 
TTTGCTCCTA TTTGCACATC TCCAAAGGAA AACGATGTCG TTGAAAAGAT GGCCATGTAC 
GTGCGGACGG CCATCGGAAC TTTGAAAATT CAAGATTTGA AATTATCTCG TAAGTTAATC 
AACTCTTGCA TAGATATTGG ATCTCGCTTA CAAGAAGATT TGGGTTATGT TTCATCAGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595694.1
CDS	559..3261
Translation	MQSSIPSVSVSVASPAMETPTKASPDSKSPNSVGAIPSSSPLASSTKASTSTPFVENCSNLLCDLASIVEDSPPTLTNTSLSPHSFSLSDMESSMSNWLNPFAFDNTMNSAPPLFTSTNMGSPNSLENSTNPLLSNCGSPNSFQNETFTGPSLNEFDADDKIQRQMKILHSVDTIDPSTVQNYPSADMSNPEVKLKTEEIITPMDTTCKPEPSAKKIKLSPSSEDSCSIPETLPFSAPKSRGSLSPSETPDFVAGPGGKPKKTAHNMIEKRYRTNLNDRICELRDAVPSLRAAAALRCGNSLDDEDLGGLTPARKLNKGTILAKATEYIRHLEAKNKELQKTNKQLSDRLAFYEDPSMAPPSNDTRAVNSVNVVSSSDYSVHQSSRPNLTQRAFTSPTLNTMGRTALNGMVGLGLFNYFGNDSSQSVYGLFALPPFLMSPFTGTVLFNMLKIGVVLLGLFYLLHDNSLFKGFKGEKKSKVSTRSSMSPSSILFRKTVFEKYCLLDHSTSTISLFFGLLIFTLKSAYGYLTHRLSALYTSSENWVYSEQQLAEVRNMEKLLDAQLMGGDAKVDRLRLLMVFASSFSLPPSSHTCALQAMYCQLIFSNTSVPSAIVSKCVAFFWNAAKKQHSKSSVHAELRELPECTANLIENSHADDVFSPNMVERLWVLAKCTRDSAQMSDSIISSLSDVLVLSPLEVLASWYAADLLDALLMESLSRKVEISEIEEIISLCPKNSSIIRHALLAKLVLFPENTADSLNEVLAAYKNTLDLCSQDKRKQSSVLKINLSKLFTLHSCLSLALQRLGYGDVSKRMYQEIFVPDSDADITPLSFIISWTALNTFAPICTSPKENDVVEKMAMYVRTAIGTLKIQDLKLSRKLINSCIDIGSRLQEDLGYVSSA

Target ORF information:

RefSeq Version	NM_001021591.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe transcription factor Sre1 (sre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021591.2

ORF Insert Sequence:

ATGCAAAGCT CAATTCCGTC AGTTTCCGTG TCGGTCGCTT CTCCTGCAAT GGAAACTCCA 
ACAAAAGCTT CGCCTGACAG CAAATCACCC AATTCAGTGG GTGCGATTCC TTCTTCATCC 
CCTTTGGCTT CATCGACAAA GGCTTCAACT TCTACTCCCT TTGTGGAGAA TTGTTCCAAT 
CTGTTGTGTG ATTTGGCTAG CATTGTCGAG GACTCACCTC CAACCTTGAC CAACACCTCG 
TTGTCTCCCC ATTCGTTTTC CCTTTCTGAT ATGGAGTCAT CAATGAGCAA TTGGCTTAAC 
CCTTTTGCGT TCGACAATAC GATGAACTCG GCGCCTCCTC TGTTTACTTC GACCAACATG 
GGCTCCCCTA ACTCGTTAGA AAACTCTACA AATCCTCTTC TCTCTAATTG CGGCTCTCCA 
AATTCATTTC AGAATGAGAC CTTTACCGGC CCATCATTGA ATGAGTTCGA TGCCGATGAT 
AAAATTCAGC GCCAAATGAA AATCCTTCAC TCTGTTGATA CAATCGATCC ATCGACTGTT 
CAAAATTATC CTTCAGCTGA TATGTCAAAT CCAGAAGTCA AATTGAAAAC CGAAGAAATT 
ATTACACCCA TGGACACCAC TTGCAAGCCT GAACCTTCTG CCAAGAAAAT AAAGTTGTCT 
CCTTCCTCGG AAGATTCTTG CTCAATTCCT GAAACATTAC CTTTTTCGGC CCCCAAGTCT 
AGAGGCTCAT TATCTCCTTC TGAAACCCCA GATTTTGTAG CCGGTCCTGG AGGAAAACCA 
AAGAAGACAG CTCATAACAT GATTGAGAAG AGATACAGAA CCAACTTGAA CGACCGAATT 
TGTGAATTGC GTGATGCAGT TCCTTCACTT CGTGCTGCTG CTGCACTTCG TTGTGGTAAT 
TCTCTGGACG ATGAGGATTT GGGGGGTTTG ACACCCGCTC GTAAGCTTAA CAAGGGTACA 
ATATTGGCAA AAGCAACTGA GTATATCAGA CATTTGGAGG CCAAGAATAA GGAACTACAG 
AAGACTAACA AGCAGCTTTC AGATAGGCTG GCTTTCTACG AAGATCCTTC GATGGCTCCT 
CCTTCTAACG ATACTCGAGC CGTTAACTCT GTTAATGTTG TTTCCTCATC TGACTATTCT 
GTTCACCAAT CTTCTCGACC AAACCTAACC CAAAGAGCTT TTACTTCCCC AACCCTAAAT 
ACCATGGGCC GCACAGCACT AAATGGCATG GTTGGACTTG GTCTATTCAA TTATTTTGGA 
AACGATTCTT CTCAATCTGT TTATGGTTTA TTCGCTCTTC CACCTTTTCT TATGTCTCCA 
TTCACAGGTA CTGTTCTCTT TAACATGCTG AAAATTGGTG TTGTTTTACT CGGTTTATTC 
TATCTTTTGC ACGATAACTC TCTTTTCAAA GGCTTCAAGG GTGAAAAGAA ATCTAAAGTT 
AGTACCAGAT CTTCCATGTC ACCATCCTCA ATCTTATTCC GAAAAACCGT ATTCGAAAAG 
TATTGTTTAT TGGACCATTC TACTTCGACT ATTTCTCTAT TTTTTGGATT ACTGATCTTC 
ACTCTTAAAT CTGCTTATGG CTATTTGACT CATCGTTTGA GTGCTCTTTA TACCTCCTCT 
GAGAATTGGG TTTATAGTGA GCAACAACTT GCTGAAGTTC GCAACATGGA AAAGCTTTTG 
GATGCCCAAT TAATGGGTGG TGATGCTAAA GTTGACCGTT TGAGGCTCTT AATGGTATTT 
GCCAGTTCAT TTTCTCTTCC TCCTTCAAGT CATACTTGTG CTTTACAAGC AATGTATTGC 
CAACTAATTT TTTCCAACAC CTCTGTCCCA TCCGCTATCG TTTCGAAGTG CGTTGCTTTC 
TTCTGGAATG CTGCAAAGAA ACAACATAGC AAAAGTTCTG TACACGCGGA GTTACGTGAG 
CTTCCTGAGT GTACTGCTAA CCTAATTGAA AACTCCCACG CTGACGACGT TTTCTCTCCG 
AATATGGTTG AGCGATTGTG GGTTCTAGCA AAGTGCACCC GTGACTCTGC TCAAATGTCA 
GATAGTATTA TTAGCTCCTT ATCTGATGTT TTGGTTTTAT CTCCTCTAGA GGTGCTTGCC 
TCATGGTATG CCGCTGATTT GTTGGATGCC CTTTTGATGG AAAGTTTGAG CCGTAAAGTT 
GAGATATCCG AGATTGAGGA AATTATTTCC CTTTGTCCTA AGAACTCCTC TATTATTCGT 
CATGCTTTAC TAGCTAAATT GGTTTTGTTC CCCGAGAATA CAGCTGATTC TTTGAATGAA 
GTCCTTGCAG CATACAAGAA CACTTTGGAT TTATGCAGCC AAGATAAACG CAAGCAGTCA 
AGCGTGTTAA AGATCAACTT ATCCAAGCTA TTCACACTCC ACTCATGCCT ATCATTAGCA 
CTCCAACGCC TTGGATACGG GGATGTTTCT AAACGTATGT ATCAGGAAAT CTTTGTACCT 
GATAGTGATG CAGACATTAC ACCTTTATCT TTTATTATTT CCTGGACCGC TTTGAACACT 
TTTGCTCCTA TTTGCACATC TCCAAAGGAA AACGATGTCG TTGAAAAGAT GGCCATGTAC 
GTGCGGACGG CCATCGGAAC TTTGAAAATT CAAGATTTGA AATTATCTCG TAAGTTAATC 
AACTCTTGCA TAGATATTGG ATCTCGCTTA CAAGAAGATT TGGGTTATGT TTCATCAGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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sre1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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