rad3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad3
Entrez Gene ID	2540719
Full Name	ATR checkpoint kinase Rad3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021264.2	NP_595357.1	ATR checkpoint kinase Rad3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MEC1	NP_009694.3
Kluyveromyces lactis	KLLA0C15785g	XP_452905.1
Eremothecium gossypii	AGOS_ABR108C	NP_983055.2
Schizosaccharomyces pombe (fission yeast)	rad3	NP_595357.1
Magnaporthe oryzae (rice blast fungus)	MGG_02656	XP_003721126.1
Neurospora crassa	NCU11188	XP_001728458.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is a DNA damage sensor kinases and plays a roll in telomere maintenance.
Title: [Telomere maintenance by DNA damage sensor kinases, ATM and ATR].

fission yeast shelterin maintains telomere length homeostasis by coordinating the differential arrival of leading (Polepsilon) and lagging (Polalpha) strand DNA polymerases at telomeres to modulate Rad3(ATR) association
Title: Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.

Tel1(ATM)/Rad3(ATR)-dependent Ccq1 Thr93 phosphorylation is a critical regulator of telomere maintenance in fission yeast.
Title: Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast.

Rad3 phosphorylates histone H2A to mobilize Brc1 to critical genomic domains during S-phase, and this pathway functions in parallel with Rqh1 DNA helicase in maintaining genome integrity.
Title: Rad3 decorates critical chromosomal domains with gammaH2A to protect genome integrity during S-Phase in fission yeast.

The N-terminus of Nbs1 contributes to the recruitment of Rad3(ATR)-Rad26(ATRIP) to telomeres.
Title: A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.

The following rad3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101297	NM_001021264.2 Latest version!	Schizosaccharomyces pombe ATR checkpoint kinase Rad3 (rad3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad3 ( NM_001021264.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595357.1 Schizosaccharomyces pombe ATR checkpoint kinase Rad3 (rad3), mRNA.

Price & Availability↑

CloneID	OSc101297
Clone ID Related Accession (Same CDS sequence)	NM_001021264.2
Accession Version	NM_001021264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	ATR checkpoint kinase Rad3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021264.1.

ATGAGCCAAC ACGCAAAAAG GAAAGCTGGG TCACTCGATC TTTCACCCAG AGGCTTAGAT 
GACAGACAGG CTTTCGGACA GCTTTTGAAA GAAGTATTAG CATTAGACAA AGAACATGAG 
TTAGGTAGAA GTAATTCTTT ACCATCTATG ACCTCCGAGC TTGTTGAAGT TTTAATTGAA 
GTTGGTCTTC TAGCTTTTAA ACATGATGAT TCAAAATCTG AATTTATCTC TCCTAAGATG 
CTAAAAGAAG CCCATCTCTC TCTACAAGCG TTAATGCTAA TCTTAAAAAG GTCTCCGACA 
GTTTTGCGGG AGATTAAATC ATCTGTTACT CTTTTGGATT GGATTTTACC CAGGACTATA 
TCATTGTTTG CTGATATTCG TTTTATTAAG TTATTTGACT CATTAAAAGA GTTTCATAAG 
CTAATTTATC AGCTAATCAG TGAAAAGTCA TTCCTATGGG ACTTATATGC TTCGTTTATG 
CGTTATTGGA AATATTATAT TACAAACGTT TCTTCTATAG TTCTCCAAAT CACTAATGCT 
ACATTCCCTT ACAAGATGCC CTCACCCAAT TCTCAACCAT TGCAGAGTAT CACCCCAAAT 
TATCCAACCC ATCGAGAGGA CAAATTTGAT TTACTTATCA TTAATATAGA GGAGGCTTGT 
ACATTTTTCT TTGAAAGTGC CCATTTTTTT GCACAATGCT CATATTTAAA GAAATCCAAT 
TTTCCTAGTC CACCTCTCTT TACAGCGTGG ACTTGGATCA AGCCATGTTT TTTTAATTTT 
GTTATTTTAT TAAAACGAAT CAGCATCGGA GACTCACAGC TCTTTCTACA TTTGCATTCA 
CGTATAGTCC AAACTTTATG CTGTTTTTCC TTGAATTTTA TATATCATGG CCTTCCCATT 
TGTGAAAAAT CTAAACATAT TTTAATGTCC TCCATCAACT TAACATTGGG ATCATTGAAG 
AAAACTTATA CAGTTGCTAA TACTGCTATA TCTCTTTTTT TTCTCTCTTT ATTTGTTTTA 
CCCAAAACTG TAGCTGGTCT ATTCTATCCT TTTGGGGTTT CCTTACTTTC TGACTTCAAG 
GTATTAGAGC AACTTGAACC AGATTCTGAT CTCAAAAAGG CAATAATATT ATTTAAGTGC 
AGATACCAAA GTTCAGAAAT AGATCAAACA ACTCTCCGTG CTTTTGGCGA AATTTGTACT 
GGTAAACTTG AAAACACGTT GTTTTCTAAC TCTGAATTAA ACCTTTTTCT TTTACATTAT 
CTTTCCTTGG ACAATGACTT GTCAAATATT CTTAAAGTGG ATTTCCAGAA TGGTCATAAC 
ATATGTACAT TTGCAAAATG GTGTATAAAC AACAACTTAG ATGAACCGTC TAATTTAAAG 
CACTTTCGTG AAATGTTAGA TTATTATAGC TCTCATAATG TTACAATAAG TGAGGACGAC 
CTGAAGAACT TCTCTTTAGT TTTGTGTACT CATGTTGCAA AGGTGAATGA GAAAACAAAT 
AGTATTTTCC GCACATATGA AGTACATGGT TGTGAAGTTT GTAACTCATT TTGTTTACTA 
TTTGATGAGC GGTCGCTCTT TAAAATTCCT TATCACGAAT TGTTTTGTGC ATTGCTAAAA 
AATCCCGACA TAATTTCCTC TTCTGTTAAA CAATCATTGT TGCTTGATGG CTTTTTTCGG 
TGGAGCCAGC ATTGCTCAAA CTTTAATAAA GAATCAATGT TAAGTTTAAG AGAATTTATT 
ATGAAAGCAT TAGCCAGTAC TTCAAGATGT TTACGTGTTG TTGCTGCAAA AGTTTTGCCC 
ATTTTCATTA AGGGACCTAA TAATCTTGAT ATAGTTGAAT TTCACAAGGA AAGTAAAGCC 
TTGATTTTTA ATACGTTGAA AATATTGGCG GTGGAAAATA CAGCTATTTT AGAAACGGTC 
ATTCTTTCCT GGATCTCCTT ATCTAGAGTG GTAGAAGAAG AAGAATTACA TTTTGTACTA 
TTGGAAGTTA TATCTTCTGT GATAAACAGC GGAATATTTT ATCAAGGCAT TGGTCTCAGC 
GCTCTGCAAC AAATTGCCTC GACGCGTCAT ATATCCGTTT GGCAATTACT TTCTCCATAT 
TGGCCAACAG TGTCCGTTGC GATTGTCCAA GGTATGGGTA AAAAACCGAA CATAGCCAGT 
TTATTTGCTC AGCTTATGAA TATTTCCGAG GGCGATTTTC TTATTCGAAC ACAGGCGTAC 
ACTTTACCAT TCCTTGTACT TACTAAAAAC AAAGCGTTAA TAGTACGTAT AGCTGAACTT 
TCACAAAGTG ATGTTGCTAC TTTGTGCCTT ACCAATATGC ATAAAATCCT TGCTTCGCTA 
CTTACTACGG ATCATCCTAA TTTGGAAGAG AGTGTGATGC TTCTTCTTTC ACTGGCCACT 
TCTGATTTTG AAAAAGTTGA TTTAACGTCT TTGTTACGCT CTGATCCTAT TTCTATTACT 
GTGGAGTTGT TACAGCTTTA TCAGAATGAT GTTCCTCATG AAAAAATTGA AAATGCTTTA 
AGAAAGGTAG CAATGATTGT CTCTCAAGTG GTTAATGACG AAGACTTGAG CAATAAGGAA 
TTACTTTATG ATTTTTTTAA TAATCACATT TTGGGTATCT TAGCAGAATT TTCTAATATC 
CTTAACGACC TGAAAGGAAA GACTTCAATT AATGAAAAGA TTAAGACAAT TGTCGGCATT 
GAAAAAATGT TATCTTTATG TGGAGGTGCA GTCAAACTTG GATTACCACA GATACTTTCT 
AATTTACAAA GTGCTTTTCA AAATGAGCAC TTAAGGTTTT ATGCAATCAA AGCTTGGTTC 
AGTTTGATAT TAGCAACCAA GGAGCCCGAG TATAGTTCAA TTGCTGGTTT AAGTCTTGTA 
ATTTTACCTC CTTTATTCCC TTATTTAGAA CCACAAGAAG CAGAGCTAGT AATTCAAATA 
TTTGATTTTA TTTCTTCTGA CACACACAAG TGCCTACAAG GATTAAAGTG GGCTATCCCC 
ACCAGTCTGG ATTCAGCGTG CTTTAGCCTT AAGGCTAAAG AAATATTCTG TTCGCTTCAA 
AATGAAGATT TTTACTCTGA GCTTCAAAGT ATAATTAAGT GTTTAACTAA CGAAAATGAG 
CCAGTTTGTT ATTTAGGTTT ACAAAAATTA GAACTTTTTT TTCAAGCCAA GGTGGACGAG 
TTACATGACA CACTAAATTT GGACATATCC AACGAAGTTC TGGACCAATT ACTAAGATGC 
CTTTTAGATT GTTGTGTAAA ATATGCTTCA ACAAATATGC AAATATCATA TCTTGCTGCA 
AAAAATCTTG GTGAATTGGG TGCGATAGAT CCCAGCCGCG CCAAGGCTCA ACATATTATT 
AAAGAAACAG TTGTTCTTGA TAACTTTGAA AACGGAGAAG AAAGTTTGAA GTTTATTCTA 
GATTTTATGC AATCGCAGTT AATTCCAGCT TTCCTTGTTA CTACTGATAC TAAAGCACAA 
GGTTTTCTTG CCTATGCTCT GCAAGAGTTT CTAAAGCTTG GTGGATTCAA GTCCGCAGTG 
ATTAATAAAA AAAAGGGACT AACTGTGGTA ACAGAACATT GGATGTCTTT GCCTGATTTA 
TCCAAACGTG TGCTTATACC ATTTTTAACT TCCAAGTATC ATTTAACACC AATCCCCAAA 
ATTGACATTC GGTACCCTAT TTATAAAGAA AATGTTACTA TTCATACTTG GATGCAGTTG 
TTTTCTCTTA AATTGATGGA GTACGCCCAT TCGCAAAACG CTGAAAAAAT ATTTGGTATT 
TGTTCGAAAG TAGTGAAAGA CCAAGAGGTT AACATTCCCT GTTTTCTTCT TCCCTTTCTT 
GTTTTAAATG TTATTTTAAC CGAGTCAGAA CTGGAAGTTA ATAAAGTCAT TGAAGAATTC 
CAGCTTGTTA TTAATCAACC GGGACCTGAT GGATTAAATT CCGTGGGGCA ACAAAGATAC 
ACCTCATTTG TAGATGTATT TTTTAAGATT GTGGATTACC TTAACAAATG GCTTCGCATG 
CGAAAGAAGA GGAATTGGGA TAGACGTTCT GCCATTGCAA GGAAAGAGAA CCGTTATATG 
TCGGTGGAAG ATGCTACCTC TCGAGAATCA TCGATCTCAA AAGTTGAGTC ATTTCTTTCT 
CGATTTCCTT CAAAAACATT AGGTATTGTC TCTTTAAATT GTGGATTTCA TGCTCGTGCA 
TTGTTTTATT GGGAGCAACA CATACGTAAT GCTACAGCTC CATATGCAGC TTTAGAGTCC 
GATTATAGAG TTTTGCAGGA AATATATGCT GGAATTGATG ATCCAGATGA AATCGAAGCA 
GTGTCTTTAA ATTTCCATGA TTACTCGTTT GATCAACAAC TCCTTTTACA TGAAAATTCA 
GGAACATGGG ACTCGGCTTT GAGTTGTTAC GAAATTATTA TTCAAAAGGA TCCTGAAAAT 
AAAAAGGCGA AAATCGGTTT GCTTAACAGC ATGCTGCAAT CGGGGCATTA TGAATCTCTT 
GTTTTGAGTT TAGATTCTTT TATAATCAAT GACAACCACG AGTATTCGAA GATGTTAAAT 
TTGGGTATTG AAGCTTCATG GCGTTCGCTA TCTATTGATT CGTTAAAAAA GTGTCTTTCA 
AAAAGCAACT TGGAATCTTT CGAAGCTAAA TTGGGTAGCA TATTTTACCA ATACCTACGG 
AAGGATTCTT TTGCTGAATT GACGGAGCGG CTGCAACCCT TGTACGTTGA TGCTGCTACA 
GCAATTGCAA ACACAGGCGC CCATTCAGCC TATGATTGTT ATGATATTTT ATCTAAGCTG 
CACGCAATTA ATGACTTTAG TAGGATTGCT GAAACTGACG GAATTGTTTC CGACAATCTT 
GATATTGTTC TTCGCCGTCG GCTTAGCCAA GTAGCTCCGT ACGGTAAATT CAAGCACCAA 
ATCCTGTCCA CTCACTTAGT TGGCTATGAA AAATTTGAAA ACACGAAGAA AACTGCTGAA 
ATATATCTCG AGATTGCAAG AATATCTCGA AAAAATGGTC AATTTCAAAG AGCCTTCAAT 
GCCATCCTCA AAGCAATGGA TTTAGATAAA CCGCTAGCAA CAATAGAGCA CGCACAATGG 
TGGTGGCATC AAGGGCAACA TCGTAAAGCT ATTTCTGAAT TGAATTTTTC GCTTAATAAC 
AACATGTTTG ATTTGGTTGA TGAGCATGAA GAAAGACCTA AAAATCGTAA AGAAACTTTA 
GGAAATCCAC TTAAAGGAAA AGTGTTCTTG AAACTTACAA AATGGCTCGG AAAAGCTGGC 
CAACTGGGAT TGAAGGATTT GGAGACGTAT TATCATAAAG CGGTAGAGAT TTACTCAGAA 
TGTGAGAATA CGCATTATTA TCTTGGCCAT CATCGAGTTT TAATGTATGA AGAAGAACAA 
AAGCTCCCAG TTAATGAACA GAGCGAACGA TTTTTAAGTG GTGAGTTAGT AACTCGCATA 
ATTAACGAAT TTGGTCGATC TTTGTACTAT GGTACAAATC ATATATATGA AAGTATGCCA 
AAATTGCTCA CACTGTGGCT TGATTTTGGG GCCGAAGAAC TTCGCTTATC TAAAGATGAC 
GGCGAAAAGT ACTTTCGTGA ACACATTATC TCTTCGAGAA AAAAATCTTT GGAACTTATG 
AATTCGAATG TTTGTCGCCT TTCTATGAAA ATTCCTCAAT ACTTTTTTCT GGTTGCATTA 
TCCCAAATGA TATCCAGAGT ATGCCATCCA AATAATAAAG TTTATAAAAT TTTGGAACAT 
ATAATTGCAA ACGTTGTAGC ATCTTATCCT GGGGAGACGC TATGGCAATT AATGGCAACA 
ATAAAATCGA CTTCTCAAAA GCGCTCGCTT CGTGGAAAAA GCATTTTAAA TGTTTTACAT 
TCTAGGAAGC TTTCTATGTC TTCCAAAGTT GATATAAAAG CACTCAGTCA ATCTGCAATT 
CTCATTACTG AAAAGTTAAT CAATTTGTGC AATACAAGGA TTAACAGTAA ATCTGTAAAA 
ATGAGCTTAA AGGATCATTT TCGGCTTTCT TTTGATGATC CGGTAGATTT AGTCATTCCT 
GCTAAATCAT TTTTAGACAT TACTTTACCA GCTAAAGATG CTAACAGAGC TAGTCATTAT 
CCATTTCCAA AAACTCAGCC TACTCTGTTG AAATTTGAGG ATGAGGTGGA TATAATGAAC 
TCTCTTCAAA AACCAAGAAA AGTGTACGTT AGAGGTACGG ATGGCAACTT ATACCCATTC 
TTGTGCAAAC CCAAAGATGA TCTTCGTAAG GATGCTAGAT TGATGGAATT TAATAATCTT 
ATTTGTAAAA TATTGAGGAA AGATCAAGAA GCGAACAGAA GGAACTTGTG TATTAGAACT 
TATGTTGTTA TTCCTTTAAA TGAAGAATGC GGATTTATCG AATGGGTAAA TCATACTCGT 
CCATTTAGAG AAATTTTGTT AAAAAGCTAT AGACAGAAAA ACATTCCCAT ATCATATCAA 
GAAATCAAAG TTGATTTAGA CTTTGCACTG CGAAGTCCTA ACCCTGGTGA TATATTTGAA 
AAGAAAATCT TACCGAAATT TCCTCCAGTT TTTTATGAGT GGTTTGTTGA ATCTTTCCCA 
GAACCAAATA ATTGGGTTAC TAGTAGACAA AACTATTGCC GAACTTTAGC AGTAATGTCA 
ATAGTTGGCT ACGTTTTGGG TTTGGGAGAT CGCCATGGCG AAAACATATT GTTTGATGAA 
TTTACAGGTG AAGCTATCCA TGTCGATTTC AACTGTCTTT TTGATAAAGG TCTTACTTTT 
GAAAAACCTG AAAAGGTGCC GTTCAGATTA ACTCATAATA TGGTAGATGC AATGGGTCCG 
ACAGGTTATG AAGGGGGTTT CAGGAAAGCT AGCGAAATAA CGATGCGGCT TCTTCGCTCA 
AACCAAGATA CATTGATGAG CGTACTAGAG TCTTTCCTAC ATGATCCTTT AGTCGAGTGG 
AATAGAAAGA AGTCGTCAAG CAAGTACCCG AATAATGAAG CAAATGAAGT TTTGGATATA 
ATTCGCAAAA AATTTCAAGG CTTTATGCCA GGGGAGACGA TACCTTTATC TATTGAAGGG 
CAAATTCAAG AATTGATCAA ATCTGCTGTC AACCCAAAAA ACCTGGTAGA AATGTACATT 
GGTTGGGCTG CTTATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595357.1
CDS	1113..8273
Translation	MSQHAKRKAGSLDLSPRGLDDRQAFGQLLKEVLALDKEHELGRSNSLPSMTSELVEVLIEVGLLAFKHDDSKSEFISPKMLKEAHLSLQALMLILKRSPTVLREIKSSVTLLDWILPRTISLFADIRFIKLFDSLKEFHKLIYQLISEKSFLWDLYASFMRYWKYYITNVSSIVLQITNATFPYKMPSPNSQPLQSITPNYPTHREDKFDLLIINIEEACTFFFESAHFFAQCSYLKKSNFPSPPLFTAWTWIKPCFFNFVILLKRISIGDSQLFLHLHSRIVQTLCCFSLNFIYHGLPICEKSKHILMSSINLTLGSLKKTYTVANTAISLFFLSLFVLPKTVAGLFYPFGVSLLSDFKVLEQLEPDSDLKKAIILFKCRYQSSEIDQTTLRAFGEICTGKLENTLFSNSELNLFLLHYLSLDNDLSNILKVDFQNGHNICTFAKWCINNNLDEPSNLKHFREMLDYYSSHNVTISEDDLKNFSLVLCTHVAKVNEKTNSIFRTYEVHGCEVCNSFCLLFDERSLFKIPYHELFCALLKNPDIISSSVKQSLLLDGFFRWSQHCSNFNKESMLSLREFIMKALASTSRCLRVVAAKVLPIFIKGPNNLDIVEFHKESKALIFNTLKILAVENTAILETVILSWISLSRVVEEEELHFVLLEVISSVINSGIFYQGIGLSALQQIASTRHISVWQLLSPYWPTVSVAIVQGMGKKPNIASLFAQLMNISEGDFLIRTQAYTLPFLVLTKNKALIVRIAELSQSDVATLCLTNMHKILASLLTTDHPNLEESVMLLLSLATSDFEKVDLTSLLRSDPISITVELLQLYQNDVPHEKIENALRKVAMIVSQVVNDEDLSNKELLYDFFNNHILGILAEFSNILNDLKGKTSINEKIKTIVGIEKMLSLCGGAVKLGLPQILSNLQSAFQNEHLRFYAIKAWFSLILATKEPEYSSIAGLSLVILPPLFPYLEPQEAELVIQIFDFISSDTHKCLQGLKWAIPTSLDSACFSLKAKEIFCSLQNEDFYSELQSIIKCLTNENEPVCYLGLQKLELFFQAKVDELHDTLNLDISNEVLDQLLRCLLDCCVKYASTNMQISYLAAKNLGELGAIDPSRAKAQHIIKETVVLDNFENGEESLKFILDFMQSQLIPAFLVTTDTKAQGFLAYALQEFLKLGGFKSAVINKKKGLTVVTEHWMSLPDLSKRVLIPFLTSKYHLTPIPKIDIRYPIYKENVTIHTWMQLFSLKLMEYAHSQNAEKIFGICSKVVKDQEVNIPCFLLPFLVLNVILTESELEVNKVIEEFQLVINQPGPDGLNSVGQQRYTSFVDVFFKIVDYLNKWLRMRKKRNWDRRSAIARKENRYMSVEDATSRESSISKVESFLSRFPSKTLGIVSLNCGFHARALFYWEQHIRNATAPYAALESDYRVLQEIYAGIDDPDEIEAVSLNFHDYSFDQQLLLHENSGTWDSALSCYEIIIQKDPENKKAKIGLLNSMLQSGHYESLVLSLDSFIINDNHEYSKMLNLGIEASWRSLSIDSLKKCLSKSNLESFEAKLGSIFYQYLRKDSFAELTERLQPLYVDAATAIANTGAHSAYDCYDILSKLHAINDFSRIAETDGIVSDNLDIVLRRRLSQVAPYGKFKHQILSTHLVGYEKFENTKKTAEIYLEIARISRKNGQFQRAFNAILKAMDLDKPLATIEHAQWWWHQGQHRKAISELNFSLNNNMFDLVDEHEERPKNRKETLGNPLKGKVFLKLTKWLGKAGQLGLKDLETYYHKAVEIYSECENTHYYLGHHRVLMYEEEQKLPVNEQSERFLSGELVTRIINEFGRSLYYGTNHIYESMPKLLTLWLDFGAEELRLSKDDGEKYFREHIISSRKKSLELMNSNVCRLSMKIPQYFFLVALSQMISRVCHPNNKVYKILEHIIANVVASYPGETLWQLMATIKSTSQKRSLRGKSILNVLHSRKLSMSSKVDIKALSQSAILITEKLINLCNTRINSKSVKMSLKDHFRLSFDDPVDLVIPAKSFLDITLPAKDANRASHYPFPKTQPTLLKFEDEVDIMNSLQKPRKVYVRGTDGNLYPFLCKPKDDLRKDARLMEFNNLICKILRKDQEANRRNLCIRTYVVIPLNEECGFIEWVNHTRPFREILLKSYRQKNIPISYQEIKVDLDFALRSPNPGDIFEKKILPKFPPVFYEWFVESFPEPNNWVTSRQNYCRTLAVMSIVGYVLGLGDRHGENILFDEFTGEAIHVDFNCLFDKGLTFEKPEKVPFRLTHNMVDAMGPTGYEGGFRKASEITMRLLRSNQDTLMSVLESFLHDPLVEWNRKKSSSKYPNNEANEVLDIIRKKFQGFMPGETIPLSIEGQIQELIKSAVNPKNLVEMYIGWAAYF

Target ORF information:

RefSeq Version	NM_001021264.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe ATR checkpoint kinase Rad3 (rad3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021264.2

ORF Insert Sequence:

ATGAGCCAAC ACGCAAAAAG GAAAGCTGGG TCACTCGATC TTTCACCCAG AGGCTTAGAT 
GACAGACAGG CTTTCGGACA GCTTTTGAAA GAAGTATTAG CATTAGACAA AGAACATGAG 
TTAGGTAGAA GTAATTCTTT ACCATCTATG ACCTCCGAGC TTGTTGAAGT TTTAATTGAA 
GTTGGTCTTC TAGCTTTTAA ACATGATGAT TCAAAATCTG AATTTATCTC TCCTAAGATG 
CTAAAAGAAG CCCATCTCTC TCTACAAGCG TTAATGCTAA TCTTAAAAAG GTCTCCGACA 
GTTTTGCGGG AGATTAAATC ATCTGTTACT CTTTTGGATT GGATTTTACC CAGGACTATA 
TCATTGTTTG CTGATATTCG TTTTATTAAG TTATTTGACT CATTAAAAGA GTTTCATAAG 
CTAATTTATC AGCTAATCAG TGAAAAGTCA TTCCTATGGG ACTTATATGC TTCGTTTATG 
CGTTATTGGA AATATTATAT TACAAACGTT TCTTCTATAG TTCTCCAAAT CACTAATGCT 
ACATTCCCTT ACAAGATGCC CTCACCCAAT TCTCAACCAT TGCAGAGTAT CACCCCAAAT 
TATCCAACCC ATCGAGAGGA CAAATTTGAT TTACTTATCA TTAATATAGA GGAGGCTTGT 
ACATTTTTCT TTGAAAGTGC CCATTTTTTT GCACAATGCT CATATTTAAA GAAATCCAAT 
TTTCCTAGTC CACCTCTCTT TACAGCGTGG ACTTGGATCA AGCCATGTTT TTTTAATTTT 
GTTATTTTAT TAAAACGAAT CAGCATCGGA GACTCACAGC TCTTTCTACA TTTGCATTCA 
CGTATAGTCC AAACTTTATG CTGTTTTTCC TTGAATTTTA TATATCATGG CCTTCCCATT 
TGTGAAAAAT CTAAACATAT TTTAATGTCC TCCATCAACT TAACATTGGG ATCATTGAAG 
AAAACTTATA CAGTTGCTAA TACTGCTATA TCTCTTTTTT TTCTCTCTTT ATTTGTTTTA 
CCCAAAACTG TAGCTGGTCT ATTCTATCCT TTTGGGGTTT CCTTACTTTC TGACTTCAAG 
GTATTAGAGC AACTTGAACC AGATTCTGAT CTCAAAAAGG CAATAATATT ATTTAAGTGC 
AGATACCAAA GTTCAGAAAT AGATCAAACA ACTCTCCGTG CTTTTGGCGA AATTTGTACT 
GGTAAACTTG AAAACACGTT GTTTTCTAAC TCTGAATTAA ACCTTTTTCT TTTACATTAT 
CTTTCCTTGG ACAATGACTT GTCAAATATT CTTAAAGTGG ATTTCCAGAA TGGTCATAAC 
ATATGTACAT TTGCAAAATG GTGTATAAAC AACAACTTAG ATGAACCGTC TAATTTAAAG 
CACTTTCGTG AAATGTTAGA TTATTATAGC TCTCATAATG TTACAATAAG TGAGGACGAC 
CTGAAGAACT TCTCTTTAGT TTTGTGTACT CATGTTGCAA AGGTGAATGA GAAAACAAAT 
AGTATTTTCC GCACATATGA AGTACATGGT TGTGAAGTTT GTAACTCATT TTGTTTACTA 
TTTGATGAGC GGTCGCTCTT TAAAATTCCT TATCACGAAT TGTTTTGTGC ATTGCTAAAA 
AATCCCGACA TAATTTCCTC TTCTGTTAAA CAATCATTGT TGCTTGATGG CTTTTTTCGG 
TGGAGCCAGC ATTGCTCAAA CTTTAATAAA GAATCAATGT TAAGTTTAAG AGAATTTATT 
ATGAAAGCAT TAGCCAGTAC TTCAAGATGT TTACGTGTTG TTGCTGCAAA AGTTTTGCCC 
ATTTTCATTA AGGGACCTAA TAATCTTGAT ATAGTTGAAT TTCACAAGGA AAGTAAAGCC 
TTGATTTTTA ATACGTTGAA AATATTGGCG GTGGAAAATA CAGCTATTTT AGAAACGGTC 
ATTCTTTCCT GGATCTCCTT ATCTAGAGTG GTAGAAGAAG AAGAATTACA TTTTGTACTA 
TTGGAAGTTA TATCTTCTGT GATAAACAGC GGAATATTTT ATCAAGGCAT TGGTCTCAGC 
GCTCTGCAAC AAATTGCCTC GACGCGTCAT ATATCCGTTT GGCAATTACT TTCTCCATAT 
TGGCCAACAG TGTCCGTTGC GATTGTCCAA GGTATGGGTA AAAAACCGAA CATAGCCAGT 
TTATTTGCTC AGCTTATGAA TATTTCCGAG GGCGATTTTC TTATTCGAAC ACAGGCGTAC 
ACTTTACCAT TCCTTGTACT TACTAAAAAC AAAGCGTTAA TAGTACGTAT AGCTGAACTT 
TCACAAAGTG ATGTTGCTAC TTTGTGCCTT ACCAATATGC ATAAAATCCT TGCTTCGCTA 
CTTACTACGG ATCATCCTAA TTTGGAAGAG AGTGTGATGC TTCTTCTTTC ACTGGCCACT 
TCTGATTTTG AAAAAGTTGA TTTAACGTCT TTGTTACGCT CTGATCCTAT TTCTATTACT 
GTGGAGTTGT TACAGCTTTA TCAGAATGAT GTTCCTCATG AAAAAATTGA AAATGCTTTA 
AGAAAGGTAG CAATGATTGT CTCTCAAGTG GTTAATGACG AAGACTTGAG CAATAAGGAA 
TTACTTTATG ATTTTTTTAA TAATCACATT TTGGGTATCT TAGCAGAATT TTCTAATATC 
CTTAACGACC TGAAAGGAAA GACTTCAATT AATGAAAAGA TTAAGACAAT TGTCGGCATT 
GAAAAAATGT TATCTTTATG TGGAGGTGCA GTCAAACTTG GATTACCACA GATACTTTCT 
AATTTACAAA GTGCTTTTCA AAATGAGCAC TTAAGGTTTT ATGCAATCAA AGCTTGGTTC 
AGTTTGATAT TAGCAACCAA GGAGCCCGAG TATAGTTCAA TTGCTGGTTT AAGTCTTGTA 
ATTTTACCTC CTTTATTCCC TTATTTAGAA CCACAAGAAG CAGAGCTAGT AATTCAAATA 
TTTGATTTTA TTTCTTCTGA CACACACAAG TGCCTACAAG GATTAAAGTG GGCTATCCCC 
ACCAGTCTGG ATTCAGCGTG CTTTAGCCTT AAGGCTAAAG AAATATTCTG TTCGCTTCAA 
AATGAAGATT TTTACTCTGA GCTTCAAAGT ATAATTAAGT GTTTAACTAA CGAAAATGAG 
CCAGTTTGTT ATTTAGGTTT ACAAAAATTA GAACTTTTTT TTCAAGCCAA GGTGGACGAG 
TTACATGACA CACTAAATTT GGACATATCC AACGAAGTTC TGGACCAATT ACTAAGATGC 
CTTTTAGATT GTTGTGTAAA ATATGCTTCA ACAAATATGC AAATATCATA TCTTGCTGCA 
AAAAATCTTG GTGAATTGGG TGCGATAGAT CCCAGCCGCG CCAAGGCTCA ACATATTATT 
AAAGAAACAG TTGTTCTTGA TAACTTTGAA AACGGAGAAG AAAGTTTGAA GTTTATTCTA 
GATTTTATGC AATCGCAGTT AATTCCAGCT TTCCTTGTTA CTACTGATAC TAAAGCACAA 
GGTTTTCTTG CCTATGCTCT GCAAGAGTTT CTAAAGCTTG GTGGATTCAA GTCCGCAGTG 
ATTAATAAAA AAAAGGGACT AACTGTGGTA ACAGAACATT GGATGTCTTT GCCTGATTTA 
TCCAAACGTG TGCTTATACC ATTTTTAACT TCCAAGTATC ATTTAACACC AATCCCCAAA 
ATTGACATTC GGTACCCTAT TTATAAAGAA AATGTTACTA TTCATACTTG GATGCAGTTG 
TTTTCTCTTA AATTGATGGA GTACGCCCAT TCGCAAAACG CTGAAAAAAT ATTTGGTATT 
TGTTCGAAAG TAGTGAAAGA CCAAGAGGTT AACATTCCCT GTTTTCTTCT TCCCTTTCTT 
GTTTTAAATG TTATTTTAAC CGAGTCAGAA CTGGAAGTTA ATAAAGTCAT TGAAGAATTC 
CAGCTTGTTA TTAATCAACC GGGACCTGAT GGATTAAATT CCGTGGGGCA ACAAAGATAC 
ACCTCATTTG TAGATGTATT TTTTAAGATT GTGGATTACC TTAACAAATG GCTTCGCATG 
CGAAAGAAGA GGAATTGGGA TAGACGTTCT GCCATTGCAA GGAAAGAGAA CCGTTATATG 
TCGGTGGAAG ATGCTACCTC TCGAGAATCA TCGATCTCAA AAGTTGAGTC ATTTCTTTCT 
CGATTTCCTT CAAAAACATT AGGTATTGTC TCTTTAAATT GTGGATTTCA TGCTCGTGCA 
TTGTTTTATT GGGAGCAACA CATACGTAAT GCTACAGCTC CATATGCAGC TTTAGAGTCC 
GATTATAGAG TTTTGCAGGA AATATATGCT GGAATTGATG ATCCAGATGA AATCGAAGCA 
GTGTCTTTAA ATTTCCATGA TTACTCGTTT GATCAACAAC TCCTTTTACA TGAAAATTCA 
GGAACATGGG ACTCGGCTTT GAGTTGTTAC GAAATTATTA TTCAAAAGGA TCCTGAAAAT 
AAAAAGGCGA AAATCGGTTT GCTTAACAGC ATGCTGCAAT CGGGGCATTA TGAATCTCTT 
GTTTTGAGTT TAGATTCTTT TATAATCAAT GACAACCACG AGTATTCGAA GATGTTAAAT 
TTGGGTATTG AAGCTTCATG GCGTTCGCTA TCTATTGATT CGTTAAAAAA GTGTCTTTCA 
AAAAGCAACT TGGAATCTTT CGAAGCTAAA TTGGGTAGCA TATTTTACCA ATACCTACGG 
AAGGATTCTT TTGCTGAATT GACGGAGCGG CTGCAACCCT TGTACGTTGA TGCTGCTACA 
GCAATTGCAA ACACAGGCGC CCATTCAGCC TATGATTGTT ATGATATTTT ATCTAAGCTG 
CACGCAATTA ATGACTTTAG TAGGATTGCT GAAACTGACG GAATTGTTTC CGACAATCTT 
GATATTGTTC TTCGCCGTCG GCTTAGCCAA GTAGCTCCGT ACGGTAAATT CAAGCACCAA 
ATCCTGTCCA CTCACTTAGT TGGCTATGAA AAATTTGAAA ACACGAAGAA AACTGCTGAA 
ATATATCTCG AGATTGCAAG AATATCTCGA AAAAATGGTC AATTTCAAAG AGCCTTCAAT 
GCCATCCTCA AAGCAATGGA TTTAGATAAA CCGCTAGCAA CAATAGAGCA CGCACAATGG 
TGGTGGCATC AAGGGCAACA TCGTAAAGCT ATTTCTGAAT TGAATTTTTC GCTTAATAAC 
AACATGTTTG ATTTGGTTGA TGAGCATGAA GAAAGACCTA AAAATCGTAA AGAAACTTTA 
GGAAATCCAC TTAAAGGAAA AGTGTTCTTG AAACTTACAA AATGGCTCGG AAAAGCTGGC 
CAACTGGGAT TGAAGGATTT GGAGACGTAT TATCATAAAG CGGTAGAGAT TTACTCAGAA 
TGTGAGAATA CGCATTATTA TCTTGGCCAT CATCGAGTTT TAATGTATGA AGAAGAACAA 
AAGCTCCCAG TTAATGAACA GAGCGAACGA TTTTTAAGTG GTGAGTTAGT AACTCGCATA 
ATTAACGAAT TTGGTCGATC TTTGTACTAT GGTACAAATC ATATATATGA AAGTATGCCA 
AAATTGCTCA CACTGTGGCT TGATTTTGGG GCCGAAGAAC TTCGCTTATC TAAAGATGAC 
GGCGAAAAGT ACTTTCGTGA ACACATTATC TCTTCGAGAA AAAAATCTTT GGAACTTATG 
AATTCGAATG TTTGTCGCCT TTCTATGAAA ATTCCTCAAT ACTTTTTTCT GGTTGCATTA 
TCCCAAATGA TATCCAGAGT ATGCCATCCA AATAATAAAG TTTATAAAAT TTTGGAACAT 
ATAATTGCAA ACGTTGTAGC ATCTTATCCT GGGGAGACGC TATGGCAATT AATGGCAACA 
ATAAAATCGA CTTCTCAAAA GCGCTCGCTT CGTGGAAAAA GCATTTTAAA TGTTTTACAT 
TCTAGGAAGC TTTCTATGTC TTCCAAAGTT GATATAAAAG CACTCAGTCA ATCTGCAATT 
CTCATTACTG AAAAGTTAAT CAATTTGTGC AATACAAGGA TTAACAGTAA ATCTGTAAAA 
ATGAGCTTAA AGGATCATTT TCGGCTTTCT TTTGATGATC CGGTAGATTT AGTCATTCCT 
GCTAAATCAT TTTTAGACAT TACTTTACCA GCTAAAGATG CTAACAGAGC TAGTCATTAT 
CCATTTCCAA AAACTCAGCC TACTCTGTTG AAATTTGAGG ATGAGGTGGA TATAATGAAC 
TCTCTTCAAA AACCAAGAAA AGTGTACGTT AGAGGTACGG ATGGCAACTT ATACCCATTC 
TTGTGCAAAC CCAAAGATGA TCTTCGTAAG GATGCTAGAT TGATGGAATT TAATAATCTT 
ATTTGTAAAA TATTGAGGAA AGATCAAGAA GCGAACAGAA GGAACTTGTG TATTAGAACT 
TATGTTGTTA TTCCTTTAAA TGAAGAATGC GGATTTATCG AATGGGTAAA TCATACTCGT 
CCATTTAGAG AAATTTTGTT AAAAAGCTAT AGACAGAAAA ACATTCCCAT ATCATATCAA 
GAAATCAAAG TTGATTTAGA CTTTGCACTG CGAAGTCCTA ACCCTGGTGA TATATTTGAA 
AAGAAAATCT TACCGAAATT TCCTCCAGTT TTTTATGAGT GGTTTGTTGA ATCTTTCCCA 
GAACCAAATA ATTGGGTTAC TAGTAGACAA AACTATTGCC GAACTTTAGC AGTAATGTCA 
ATAGTTGGCT ACGTTTTGGG TTTGGGAGAT CGCCATGGCG AAAACATATT GTTTGATGAA 
TTTACAGGTG AAGCTATCCA TGTCGATTTC AACTGTCTTT TTGATAAAGG TCTTACTTTT 
GAAAAACCTG AAAAGGTGCC GTTCAGATTA ACTCATAATA TGGTAGATGC AATGGGTCCG 
ACAGGTTATG AAGGGGGTTT CAGGAAAGCT AGCGAAATAA CGATGCGGCT TCTTCGCTCA 
AACCAAGATA CATTGATGAG CGTACTAGAG TCTTTCCTAC ATGATCCTTT AGTCGAGTGG 
AATAGAAAGA AGTCGTCAAG CAAGTACCCG AATAATGAAG CAAATGAAGT TTTGGATATA 
ATTCGCAAAA AATTTCAAGG CTTTATGCCA GGGGAGACGA TACCTTTATC TATTGAAGGG 
CAAATTCAAG AATTGATCAA ATCTGCTGTC AACCCAAAAA ACCTGGTAGA AATGTACATT 
GGTTGGGCTG CTTATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rad3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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