ORF » Species Summary » Schizosaccharomyces pombe » git5 cDNA ORF clone

git5 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following git5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the git5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101216 NM_001021508.2
Latest version!		 Schizosaccharomyces pombe heterotrimeric G protein beta subunit Git5 (git5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    git5 ( NM_001021508.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595613.1 Schizosaccharomyces pombe heterotrimeric G protein beta subunit Git5 (git5), mRNA.
    Price & Availability↑
    CloneID OSc101216
    Clone ID Related Accession (Same CDS sequence) NM_001021508.2
    Accession Version NM_001021508.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product heterotrimeric G protein beta subunit Git5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021508.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTCTG GGTCAAGAGT AAACGTCAAC ATTCAAGGGA CTCGAGTTCT CAAAAACAAG 
    CTGGGAAAAA TTCCTGACAT CGACATATCT ACAGATGGAA AGTACTTATT ATCTGCATCA 
    ACGAATGATG TTCTGTTAGT TTGGGATTTG CACACTTCCA ACAAAGTTGC GTTCTTTGAA 
    GCACCAAGCG TATGGATTAT GACTTGTGCA TTCTCACCCT CCACTAAGAG CATAGCAGCT 
    GGAGGGCTGA ACAACTTTTG CGTAGTATAC GATACCAGTG TTCCAGATGC GGATCCGGTC 
    GAATTGGTTG GGCATGCAGG ATTTGTTTCG TGCTGTAAGT ATGTCGATGA CGGTCATTTG 
    CTTACGGGTT CTGGCGATAA AACTTGCATG TTTTGGGACA TAGAGCAGGC AAAAGCCATT 
    AGCGTTTTGA AAGGTCATGA AATGGATATT GTCTCTCTAG ATTTCCTACC CTCAAATCCC 
    AATTTATTTG TTACCGGAGG CTGTGACAAA TTAGCCAAGC TCTGGGACCT TCGGGCTGCT 
    TATTGTTGTG CCACATTTCC CGGAAATACA TCGGATATTA ATTCCATTTC CTTTTTTCCT 
    TCTAACGCTG ATTTTGTTAC GGGTGCAGAG GATGGTATCG CTCGGTGCTT TGACATTCGT 
    GCTTCTGCTG AGATATTCCA GTATTCATCT CCATCATCCT CCCCCATTAA TTCAGTCTTA 
    TTTTCCAAGT CTGGAAAACT TCTTTTTATC GCAAAAGATA AAACATGCGA GGTTTGGGAT 
    TCCATCTCAA GCAAAACCAT CACATCATTA ACAGGCCATG AAAATCGAAT TAGCTCTTTA 
    GCTTTAACTT CTGATGGAAC AATGTTGGCA ACTGGCTCTT GGGACGAATG TGTTCGTCTC 
    TGGTCTTCGT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595613.1
    CDS498..1415
    Translation

    Target ORF information:

    RefSeq Version NM_001021508.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe heterotrimeric G protein beta subunit Git5 (git5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001021508.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTCTG GGTCAAGAGT AAACGTCAAC ATTCAAGGGA CTCGAGTTCT CAAAAACAAG 
    CTGGGAAAAA TTCCTGACAT CGACATATCT ACAGATGGAA AGTACTTATT ATCTGCATCA 
    ACGAATGATG TTCTGTTAGT TTGGGATTTG CACACTTCCA ACAAAGTTGC GTTCTTTGAA 
    GCACCAAGCG TATGGATTAT GACTTGTGCA TTCTCACCCT CCACTAAGAG CATAGCAGCT 
    GGAGGGCTGA ACAACTTTTG CGTAGTATAC GATACCAGTG TTCCAGATGC GGATCCGGTC 
    GAATTGGTTG GGCATGCAGG ATTTGTTTCG TGCTGTAAGT ATGTCGATGA CGGTCATTTG 
    CTTACGGGTT CTGGCGATAA AACTTGCATG TTTTGGGACA TAGAGCAGGC AAAAGCCATT 
    AGCGTTTTGA AAGGTCATGA AATGGATATT GTCTCTCTAG ATTTCCTACC CTCAAATCCC 
    AATTTATTTG TTACCGGAGG CTGTGACAAA TTAGCCAAGC TCTGGGACCT TCGGGCTGCT 
    TATTGTTGTG CCACATTTCC CGGAAATACA TCGGATATTA ATTCCATTTC CTTTTTTCCT 
    TCTAACGCTG ATTTTGTTAC GGGTGCAGAG GATGGTATCG CTCGGTGCTT TGACATTCGT 
    GCTTCTGCTG AGATATTCCA GTATTCATCT CCATCATCCT CCCCCATTAA TTCAGTCTTA 
    TTTTCCAAGT CTGGAAAACT TCTTTTTATC GCAAAAGATA AAACATGCGA GGTTTGGGAT 
    TCCATCTCAA GCAAAACCAT CACATCATTA ACAGGCCATG AAAATCGAAT TAGCTCTTTA 
    GCTTTAACTT CTGATGGAAC AATGTTGGCA ACTGGCTCTT GGGACGAATG TGTTCGTCTC 
    TGGTCTTCGT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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