cut4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cut4
Entrez Gene ID	2539639
Full Name	anaphase-promoting complex subunit Apc1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021067.2	NP_595158.1	anaphase-promoting complex subunit Apc1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cut4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cut4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101464	NM_001021067.2 Latest version!	Schizosaccharomyces pombe anaphase-promoting complex subunit Apc1 (cut4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cut4 ( NM_001021067.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595158.1 Schizosaccharomyces pombe anaphase-promoting complex subunit Apc1 (cut4), mRNA.

Price & Availability↑

CloneID	OSc101464
Clone ID Related Accession (Same CDS sequence)	NM_001021067.2
Accession Version	NM_001021067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	anaphase-promoting complex subunit Apc1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021067.1.

ATGCTAGAAT TAAAAACAAA TGTTATTCAA GACCCTGAAT TAAAGGATTC ATTAGATTTG 
CAAGAGAATG ATCGGGTTGA AATCATTGGA GATGCTGTGC ATTATATTGT GAATGACCAT 
TTAAATCGTG TATTTAATTA TACCGTAGAT CAACAAAAGA TACATGCTGC CCTTATTACT 
ACATTTGCAT CTGGAAAAAA GGCAATTGTT GTGATTCTGG ACGACATTGG ATATGTATAC 
TATGTGGGCG ACAATAATAA TGATTCTTAT ATTATAAACG TTCCTTTTTC AACAGAAAGC 
GCATGGAGTT CACCAAGTGG ACTATATCTT CAGCGTCATC GTTCGAGCGA TGAGAATCTG 
AATACTGACT TGCCTCATAT TTTTTGTCTA AACGATCCTT TAGATGAACT TACGTTAATA 
AAATTTGACG GAAAAAAAAT ACTATCTCTG TTTGATTCAA TCGTTTATGT CGTGGGAGAG 
ATCGTCGTAA CACATAATAA AAAAGAAAAA AAATTGAGTT TTTGGAGGAG TCGCTTTATT 
GATCCAGAGT CAGACCAGTC AATAAAAAGC TCACGTAACA GAAGAAGGGA AAGCTCTTTT 
TCTCGTGAAA AGAATCCTGA CTTAACTCGC TCTGATTCTA TCCATTATAC CGCCAATACT 
CGTCTTTCTG AAAAATTCGA GGAACAAGGA CTCGCTTATT CGACCATATT TTCTCACATA 
GAGTCTTTTC CTATCACCGG AAGTACTTCG TTTGATTCAA TCCTTGGAAA TGGTGTTTTG 
GTCATCACCA CTTTAGTGAA AGAGCTTGAA AAAGGCTACA TGATGTTGTT TCGCCTTGTT 
CGTGACAGAT CTCCTTACTT CTTAGATTCG CTTCAACTAC ATGCAATAAA TTTGAGTGCT 
ATTAAATCTA AGCATTTGCA AAAAATTGTT GTTCTGTCTT CAAAGGGGAA GGTGAGTCTC 
GAGTCTCCAA TGTCGCCATC TTTGCCTATT GAGGGTACCT TTCGTTCATT TCGTGTGCAT 
GGTGCCACAC TTTACTTAGA GGACACCGAT GGAGTGCAGA GGTATATATC TCTAGATAAC 
AGAGCATCCA ATAGCTTGGT AAAATGGTGT TTAAGTGTTA TTCGTTATGT TTTGCCCTTG 
CGTGAATATG AAATTTTTTA TACTGGACAT CTTTACGCGC TTTTTGCTTT TAAGCTTAGC 
CATGATGAGG CTTTCATATC TTCAATACTC GCCTGTTTCA CTTTCTTTTC TCGAGATAAA 
GTTCATGTTG AACCAATTGA AGATTGCAAT GAAGCTTACA GTCTGTCCTC TAAGTTTCAT 
TTTAAAAAAG AAATACTTAT TGCTTCTCAG CTCTCATCTC ATCTTGATTA CTCAACATTC 
AAAAATTACT TGATGCCCTT AGCTATTACC TTACATTTTA TATCTGAAGA ATTGCGGTTG 
GATTCGGTTG TAAAGCCTCG TAAAGATCAG CTTGTAGCTT TATTACTTCA AATCACCACG 
TGGTTGAAGT GGCCACGGTA TTGCGAGTAT TATAACTTTG ATATCGCTGA AACATTTCTA 
TCTATACCCT TATCGATTCA AGTTGATGTT GAGGAACCTG TTGGTCCAAC ATCGATTTTA 
CAGTGGATTA TTGAATGTTT AAGGTCGCAA TCTACAGTAC CTTTTTATGG CTTGGAAAGT 
TATGGGCTAC CACATTCTTG CTCTACTATG TTTCCCCAAA CCCTTTCTTT AATGCAGTTA 
CTCGATTGCT TGCTAAATCC TAATATGACC CTTCAAAACT TAGTTGAAGA AATGGTACGG 
TTAGGAATTT CAAGAAAGAG GTGTGAAAGA TACCCATTTG GAATTTTATG CATTATTTTT 
ACAGTACTCG AGATTGCCGC TGAAGAGTAT TCTCCGAATT GGGAAAGCGA AGAGCTTCGA 
TTAGTCAATC GCCTTGACGT TGATTCATTC TTACATCCAA AGACACCTAA ATGGGTTTTT 
AATAAGCAGG ATCAAGAAGT CAAAGAAATA AAAGCACTCA CTAGTACAGT TACCGATTCT 
ACACTTGTTG ACACGCAGTC GTTTCATCCT TATAAGGTTG TAACGGATAT GATTTTTAGA 
GAGGATCGAC GACTGGCAGA AGTGAATAAA CTTTTAAATT ATTCAAGTCA AATCACCATA 
ATGACTGAAC ACTTTGATGT CGACTTGAGT TCAGTACCAA TGCAACAAAA AGTTGCTCAA 
TGCATTTGCG TTAGAACATT GTCCGTGCCA ATTGGAGCGG GTATGCTTAC GTATGGTAGT 
AAAAATCCTT TACCTACGGA AAAGGTCACT CCACGATTAT TTAATTTTAC ATTGCACTTG 
CATCCTGGAA CTTTAATTAT ACAACCTAAT AAAGAGTTCG TAACACAGGA ATTAACCGAG 
TGGCCAGAGT TTAATGTTGG CGTTGCATTA GGATTATCGA TATCTAAGTT TTCAAAGGAA 
ATAAACACTA GTTGGATTAT GTTCAATCGA CCTGAAACTT TGACTGCTTA CCATGCGGGA 
TTTCTTTTTG GTCTAGGGCT AAACGGTCAT TTAAAGGCCC TTGCTACCTG GCATTCTTTT 
ATTTATCTTA CTAGCAAGCA TGATACAACC AGTATAGGCC TTTTACTTGG ACTTGCATCC 
TCGTATTTGG GGAGTATGGA TGCAAAGGTT ACAAAATTGC TAAGTGTGCA CATTTCAGCA 
TTACTGCCAG TTGGATCTAA TGAATTGAAT ATTTCACCAT TAACTCAGAC TGCAGGAATT 
CTAGGAATTG GTTTATTATT TCATGATTCG TGTCATAGAC GCATGTCTGA AGTGACCATG 
GAAGAGATTT TGGCTTCTAA TGAAAGCGAA CTAAAGAATG AAGGCTATAA GTTAGCTGCT 
GGATTTTCTC TTGGTTTAAT TAATTTAGGG AGAGGATCTA ATCTTCCAGG CATGTCTGAT 
TTAAAACTCG TTTCAAGGCT ACAAGTGGGA ATATCTAGTC AGGCTACATT TCAGTCTTTA 
GAAGCCGGTT CTCCCGGGGC CATTATGGCT TTGACTATGA TATATATGAA GACTAATGAT 
TTGGAAGTTG CGAAAAAAAT TGATATTCCT AAATCGCGAT ATCTTCTTGA TTTCTATCGT 
CCAGATCTAA TACTTTTGCG AGTTGCCGGT AAAAACTTAA TTATGTGGGA TGAAGTCAAA 
GCAGATTATG AATGGGTGAA ATATCAAATT CCTGATATAA TGCTCTCACA ATTTGATTTA 
CAGGAAAAAA AGGTTCTCTC TTCTGACGAC TTGCTGCTTT ATAATGTCTT AGCAGGGATT 
TGTTTTTCTT TAGGTTTAAG GTTTGCTGGA ACGGGTAATC CGAAAGCCAA GGAAATTCTC 
ATCAACTTTT TAGATAGCTT CATTCGGTTG TGTCATCTGC CTGCTAAAAC ACACGATGAA 
AGAGTAACTG CAGTGACTGT AATTCGCTGT ACACAAATTG TCGCTTTGTC TTCTTCTTGT 
GTTATGGCGG GTTATTGTGA TCTAGATGTT TTGAGAAGGT TAAGAGTACT ACATGGGAGA 
ATGGAGCCTG TGAACTACGG GGCTCAAATG GCTACTCATA TGGCGCTCGG AATTCTTTCG 
CTAGGTGGAG GAAGGTATAG TTTGTCTCGC AGTAATTTAG CAATCGCGGC TTTGTTAATA 
TCATTTTATC CGCAATTTCC CAGGACTACT CAAGATAATC GTGCACATTT GCAAGCTGCT 
CGAAATTTGT GGGCTTTAGC TGTGGAAGAA CGTTGTATTA TCCCGAGAAA TCAAGATACC 
AAACAGCCAT GCATAGTGCC ATTAAATGTT GTTCAAAAAA GCGGCGCAGT TCAAAAACTA 
GAAGCGCCTA TACTTTTGCC GCCGTACGAT AGCATTAGTT CAGTGTCGAC GTTGGGTGAT 
AAGTATTGGA ATTTAAAAAT TGATTTGGAC AATAACAGTG ATTACCGTGA ACTATTAAGG 
GAATCACAAA CACTGACACT GATGCCTTAT GACCGAACAA GTTCAAAAGA AGAGCCACTT 
AATCTATTCC CTAAGCTTAA AGATACCTCA AGCCCACTTT GGAACTTGGT AAAAACAAGC 
AGGTTGTTCC AAAGCTCGAA TAGCCCCTTA AACGTTGCAT CGTTACAAGA ATCAAATAAT 
AAAACTTCTC TAGGCGTTAA ATTACTCCTG TCTATGGACT TTGATAATTT AACTAGAGAC 
CGTTTATTAA GTTTGCAAAT ACTGTTACAA TTTTTTGAGT CTTGTTGGAC TGGTGTGCTC 
TTGAATAAGT TTCACTCCCG TCAATATCTT TTCCTTTCTC GTGATTTGGT AGAAGATTTG 
AGTTTGAGAG TATGGGAGTA TGTGTATTCT CATAATCATA ATGAAGAATC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595158.1
CDS	65..4441
Translation	MLELKTNVIQDPELKDSLDLQENDRVEIIGDAVHYIVNDHLNRVFNYTVDQQKIHAALITTFASGKKAIVVILDDIGYVYYVGDNNNDSYIINVPFSTESAWSSPSGLYLQRHRSSDENLNTDLPHIFCLNDPLDELTLIKFDGKKILSLFDSIVYVVGEIVVTHNKKEKKLSFWRSRFIDPESDQSIKSSRNRRRESSFSREKNPDLTRSDSIHYTANTRLSEKFEEQGLAYSTIFSHIESFPITGSTSFDSILGNGVLVITTLVKELEKGYMMLFRLVRDRSPYFLDSLQLHAINLSAIKSKHLQKIVVLSSKGKVSLESPMSPSLPIEGTFRSFRVHGATLYLEDTDGVQRYISLDNRASNSLVKWCLSVIRYVLPLREYEIFYTGHLYALFAFKLSHDEAFISSILACFTFFSRDKVHVEPIEDCNEAYSLSSKFHFKKEILIASQLSSHLDYSTFKNYLMPLAITLHFISEELRLDSVVKPRKDQLVALLLQITTWLKWPRYCEYYNFDIAETFLSIPLSIQVDVEEPVGPTSILQWIIECLRSQSTVPFYGLESYGLPHSCSTMFPQTLSLMQLLDCLLNPNMTLQNLVEEMVRLGISRKRCERYPFGILCIIFTVLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKTPKWVFNKQDQEVKEIKALTSTVTDSTLVDTQSFHPYKVVTDMIFREDRRLAEVNKLLNYSSQITIMTEHFDVDLSSVPMQQKVAQCICVRTLSVPIGAGMLTYGSKNPLPTEKVTPRLFNFTLHLHPGTLIIQPNKEFVTQELTEWPEFNVGVALGLSISKFSKEINTSWIMFNRPETLTAYHAGFLFGLGLNGHLKALATWHSFIYLTSKHDTTSIGLLLGLASSYLGSMDAKVTKLLSVHISALLPVGSNELNISPLTQTAGILGIGLLFHDSCHRRMSEVTMEEILASNESELKNEGYKLAAGFSLGLINLGRGSNLPGMSDLKLVSRLQVGISSQATFQSLEAGSPGAIMALTMIYMKTNDLEVAKKIDIPKSRYLLDFYRPDLILLRVAGKNLIMWDEVKADYEWVKYQIPDIMLSQFDLQEKKVLSSDDLLLYNVLAGICFSLGLRFAGTGNPKAKEILINFLDSFIRLCHLPAKTHDERVTAVTVIRCTQIVALSSSCVMAGYCDLDVLRRLRVLHGRMEPVNYGAQMATHMALGILSLGGGRYSLSRSNLAIAALLISFYPQFPRTTQDNRAHLQAARNLWALAVEERCIIPRNQDTKQPCIVPLNVVQKSGAVQKLEAPILLPPYDSISSVSTLGDKYWNLKIDLDNNSDYRELLRESQTLTLMPYDRTSSKEEPLNLFPKLKDTSSPLWNLVKTSRLFQSSNSPLNVASLQESNNKTSLGVKLLLSMDFDNLTRDRLLSLQILLQFFESCWTGVLLNKFHSRQYLFLSRDLVEDLSLRVWEYVYSHNHNEESV

Target ORF information:

RefSeq Version	NM_001021067.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe anaphase-promoting complex subunit Apc1 (cut4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021067.2

ORF Insert Sequence:

ATGCTAGAAT TAAAAACAAA TGTTATTCAA GACCCTGAAT TAAAGGATTC ATTAGATTTG 
CAAGAGAATG ATCGGGTTGA AATCATTGGA GATGCTGTGC ATTATATTGT GAATGACCAT 
TTAAATCGTG TATTTAATTA TACCGTAGAT CAACAAAAGA TACATGCTGC CCTTATTACT 
ACATTTGCAT CTGGAAAAAA GGCAATTGTT GTGATTCTGG ACGACATTGG ATATGTATAC 
TATGTGGGCG ACAATAATAA TGATTCTTAT ATTATAAACG TTCCTTTTTC AACAGAAAGC 
GCATGGAGTT CACCAAGTGG ACTATATCTT CAGCGTCATC GTTCGAGCGA TGAGAATCTG 
AATACTGACT TGCCTCATAT TTTTTGTCTA AACGATCCTT TAGATGAACT TACGTTAATA 
AAATTTGACG GAAAAAAAAT ACTATCTCTG TTTGATTCAA TCGTTTATGT CGTGGGAGAG 
ATCGTCGTAA CACATAATAA AAAAGAAAAA AAATTGAGTT TTTGGAGGAG TCGCTTTATT 
GATCCAGAGT CAGACCAGTC AATAAAAAGC TCACGTAACA GAAGAAGGGA AAGCTCTTTT 
TCTCGTGAAA AGAATCCTGA CTTAACTCGC TCTGATTCTA TCCATTATAC CGCCAATACT 
CGTCTTTCTG AAAAATTCGA GGAACAAGGA CTCGCTTATT CGACCATATT TTCTCACATA 
GAGTCTTTTC CTATCACCGG AAGTACTTCG TTTGATTCAA TCCTTGGAAA TGGTGTTTTG 
GTCATCACCA CTTTAGTGAA AGAGCTTGAA AAAGGCTACA TGATGTTGTT TCGCCTTGTT 
CGTGACAGAT CTCCTTACTT CTTAGATTCG CTTCAACTAC ATGCAATAAA TTTGAGTGCT 
ATTAAATCTA AGCATTTGCA AAAAATTGTT GTTCTGTCTT CAAAGGGGAA GGTGAGTCTC 
GAGTCTCCAA TGTCGCCATC TTTGCCTATT GAGGGTACCT TTCGTTCATT TCGTGTGCAT 
GGTGCCACAC TTTACTTAGA GGACACCGAT GGAGTGCAGA GGTATATATC TCTAGATAAC 
AGAGCATCCA ATAGCTTGGT AAAATGGTGT TTAAGTGTTA TTCGTTATGT TTTGCCCTTG 
CGTGAATATG AAATTTTTTA TACTGGACAT CTTTACGCGC TTTTTGCTTT TAAGCTTAGC 
CATGATGAGG CTTTCATATC TTCAATACTC GCCTGTTTCA CTTTCTTTTC TCGAGATAAA 
GTTCATGTTG AACCAATTGA AGATTGCAAT GAAGCTTACA GTCTGTCCTC TAAGTTTCAT 
TTTAAAAAAG AAATACTTAT TGCTTCTCAG CTCTCATCTC ATCTTGATTA CTCAACATTC 
AAAAATTACT TGATGCCCTT AGCTATTACC TTACATTTTA TATCTGAAGA ATTGCGGTTG 
GATTCGGTTG TAAAGCCTCG TAAAGATCAG CTTGTAGCTT TATTACTTCA AATCACCACG 
TGGTTGAAGT GGCCACGGTA TTGCGAGTAT TATAACTTTG ATATCGCTGA AACATTTCTA 
TCTATACCCT TATCGATTCA AGTTGATGTT GAGGAACCTG TTGGTCCAAC ATCGATTTTA 
CAGTGGATTA TTGAATGTTT AAGGTCGCAA TCTACAGTAC CTTTTTATGG CTTGGAAAGT 
TATGGGCTAC CACATTCTTG CTCTACTATG TTTCCCCAAA CCCTTTCTTT AATGCAGTTA 
CTCGATTGCT TGCTAAATCC TAATATGACC CTTCAAAACT TAGTTGAAGA AATGGTACGG 
TTAGGAATTT CAAGAAAGAG GTGTGAAAGA TACCCATTTG GAATTTTATG CATTATTTTT 
ACAGTACTCG AGATTGCCGC TGAAGAGTAT TCTCCGAATT GGGAAAGCGA AGAGCTTCGA 
TTAGTCAATC GCCTTGACGT TGATTCATTC TTACATCCAA AGACACCTAA ATGGGTTTTT 
AATAAGCAGG ATCAAGAAGT CAAAGAAATA AAAGCACTCA CTAGTACAGT TACCGATTCT 
ACACTTGTTG ACACGCAGTC GTTTCATCCT TATAAGGTTG TAACGGATAT GATTTTTAGA 
GAGGATCGAC GACTGGCAGA AGTGAATAAA CTTTTAAATT ATTCAAGTCA AATCACCATA 
ATGACTGAAC ACTTTGATGT CGACTTGAGT TCAGTACCAA TGCAACAAAA AGTTGCTCAA 
TGCATTTGCG TTAGAACATT GTCCGTGCCA ATTGGAGCGG GTATGCTTAC GTATGGTAGT 
AAAAATCCTT TACCTACGGA AAAGGTCACT CCACGATTAT TTAATTTTAC ATTGCACTTG 
CATCCTGGAA CTTTAATTAT ACAACCTAAT AAAGAGTTCG TAACACAGGA ATTAACCGAG 
TGGCCAGAGT TTAATGTTGG CGTTGCATTA GGATTATCGA TATCTAAGTT TTCAAAGGAA 
ATAAACACTA GTTGGATTAT GTTCAATCGA CCTGAAACTT TGACTGCTTA CCATGCGGGA 
TTTCTTTTTG GTCTAGGGCT AAACGGTCAT TTAAAGGCCC TTGCTACCTG GCATTCTTTT 
ATTTATCTTA CTAGCAAGCA TGATACAACC AGTATAGGCC TTTTACTTGG ACTTGCATCC 
TCGTATTTGG GGAGTATGGA TGCAAAGGTT ACAAAATTGC TAAGTGTGCA CATTTCAGCA 
TTACTGCCAG TTGGATCTAA TGAATTGAAT ATTTCACCAT TAACTCAGAC TGCAGGAATT 
CTAGGAATTG GTTTATTATT TCATGATTCG TGTCATAGAC GCATGTCTGA AGTGACCATG 
GAAGAGATTT TGGCTTCTAA TGAAAGCGAA CTAAAGAATG AAGGCTATAA GTTAGCTGCT 
GGATTTTCTC TTGGTTTAAT TAATTTAGGG AGAGGATCTA ATCTTCCAGG CATGTCTGAT 
TTAAAACTCG TTTCAAGGCT ACAAGTGGGA ATATCTAGTC AGGCTACATT TCAGTCTTTA 
GAAGCCGGTT CTCCCGGGGC CATTATGGCT TTGACTATGA TATATATGAA GACTAATGAT 
TTGGAAGTTG CGAAAAAAAT TGATATTCCT AAATCGCGAT ATCTTCTTGA TTTCTATCGT 
CCAGATCTAA TACTTTTGCG AGTTGCCGGT AAAAACTTAA TTATGTGGGA TGAAGTCAAA 
GCAGATTATG AATGGGTGAA ATATCAAATT CCTGATATAA TGCTCTCACA ATTTGATTTA 
CAGGAAAAAA AGGTTCTCTC TTCTGACGAC TTGCTGCTTT ATAATGTCTT AGCAGGGATT 
TGTTTTTCTT TAGGTTTAAG GTTTGCTGGA ACGGGTAATC CGAAAGCCAA GGAAATTCTC 
ATCAACTTTT TAGATAGCTT CATTCGGTTG TGTCATCTGC CTGCTAAAAC ACACGATGAA 
AGAGTAACTG CAGTGACTGT AATTCGCTGT ACACAAATTG TCGCTTTGTC TTCTTCTTGT 
GTTATGGCGG GTTATTGTGA TCTAGATGTT TTGAGAAGGT TAAGAGTACT ACATGGGAGA 
ATGGAGCCTG TGAACTACGG GGCTCAAATG GCTACTCATA TGGCGCTCGG AATTCTTTCG 
CTAGGTGGAG GAAGGTATAG TTTGTCTCGC AGTAATTTAG CAATCGCGGC TTTGTTAATA 
TCATTTTATC CGCAATTTCC CAGGACTACT CAAGATAATC GTGCACATTT GCAAGCTGCT 
CGAAATTTGT GGGCTTTAGC TGTGGAAGAA CGTTGTATTA TCCCGAGAAA TCAAGATACC 
AAACAGCCAT GCATAGTGCC ATTAAATGTT GTTCAAAAAA GCGGCGCAGT TCAAAAACTA 
GAAGCGCCTA TACTTTTGCC GCCGTACGAT AGCATTAGTT CAGTGTCGAC GTTGGGTGAT 
AAGTATTGGA ATTTAAAAAT TGATTTGGAC AATAACAGTG ATTACCGTGA ACTATTAAGG 
GAATCACAAA CACTGACACT GATGCCTTAT GACCGAACAA GTTCAAAAGA AGAGCCACTT 
AATCTATTCC CTAAGCTTAA AGATACCTCA AGCCCACTTT GGAACTTGGT AAAAACAAGC 
AGGTTGTTCC AAAGCTCGAA TAGCCCCTTA AACGTTGCAT CGTTACAAGA ATCAAATAAT 
AAAACTTCTC TAGGCGTTAA ATTACTCCTG TCTATGGACT TTGATAATTT AACTAGAGAC 
CGTTTATTAA GTTTGCAAAT ACTGTTACAA TTTTTTGAGT CTTGTTGGAC TGGTGTGCTC 
TTGAATAAGT TTCACTCCCG TCAATATCTT TTCCTTTCTC GTGATTTGGT AGAAGATTTG 
AGTTTGAGAG TATGGGAGTA TGTGTATTCT CATAATCATA ATGAAGAATC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cut4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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