brl2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following brl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99355 NM_001022838.2
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Schizosaccharomyces pombe ubiquitin-protein ligase E3 Brl2 (brl2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99355
    Clone ID Related Accession (Same CDS sequence) NM_001022838.2
    Accession Version NM_001022838.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ubiquitin-protein ligase E3 Brl2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022838.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTATCAGA ATGGTAAACC AGATGCCCCC ACAATCTTAG GCCAGAAAAG AGAACTTGAA 
    GATGTAGAAA TACAAGATGA TGATATTCAG GAAGTCTCTA AAGAAGACTT ATTAAAGGAT
    GTAAGAGTGA GGAGCATTCA ATTTGATGAG TTAGAAAGTA AAATAGAGGG ACTTCAAAAT
    TTAGCAGAAG AGAAATTAAA AGTTTTAGCT ACTTTGGTCT CTTGGTGGCC TGAAATACTT
    CAGCAGTTTA GCGTTGTGTT TCAAGGCAAT GAGCTGAAGG ACTTCGAGAG TGAAGGTGTT
    TTTTCGATTT TGGAGAAATT CCCGGAGCTT TCATACTTCA ATGACGCTGT GAAAAATAAT
    AAAACTAAAG CCTTGAGCAT CATTCAAAAA CTCTTGTCTA CTGTTGACTC AAGCACAAAT
    TCGGTCTCCA GAGATCCTTT TTCAGTTTTA TCAATTGATG ACTCTGCCCT TACTGAGAAA
    TTAAACACCA TTAATTTGGA TATTGACAAG ATTTTAGATG AATTGGATAC CACTCGGTCT
    CAGCTTCATT CAATTATAAA ACTACCAGAT CGCTCGTCTA GTTTTACTTT GCAATGCATT
    AATGAATCAG TGAGACCCCA GTCTACTAAA GTGAAAGAGG AAGCAACCAC CTCCTCTAAG
    GGGAAAGATG AAGAAAAGAA AGTTAGCACG GTTGAACAAC GTACTCAACT GCAACAATTA
    TCCAGGCTTC AAGACCAACA AAATGGTTTA ATGGAGTCTC GCTCACAAAG CCTTAAAATT
    TTGGATTCGA ACGTTAATGA AATGGATAAA TTAATTATGG AACGAGAAAA CGCATTAAAC
    AACGTCGAAA CTACAAACTT GAAAAAATAC TCTTCCTTTT TAGCACTTAA AGAAGCTGTT
    TCTATGACAT CTGAGCAGTT ACGCGTATTA GAGCACTTAT TGTCCGAATG TTCTCACGAA
    ATAAATGTAT TGTCGCAACA GTCAAAGAAT TTTAACGGTG TTTTTGAATC TTCCTATCAG
    CCGTTAATAA ACGATCTTGA TCATCAAATA TCTGTTATGC AAAACGATGA AAAAAGAATT
    AATAACGCAA AGACTGAATT AAGCTTATCC CTTGAAAAGA AGTTGGAAGC TAAAAAGCAA
    AAAGAGAAGG TCTACAAAGA CAAGTTAGAT GAACTTGCGA ATTTAGAGAC AATGGTATTG
    GAAAAGAAAA AAGCGGTGGC TACCCGTGAA GCTGCAAACA AAATCCGGCT CGTAGACCTT
    AACGATTTGG AGTTACAAAA AGATCTGTCA ACTTACCTTT CTAAAGAGTT GGCTTCTACT
    GAAAAAGCAT TTAGGTTGGT AAAACAGCAA ACTGTGAAAA GTAGCCACTC CCATTATCAA
    GAATTGATAA CCAAATTTAG TGTCGAAAAG GAAAAGGCGG AGCAAAAATA TTTTCTGACC
    ATGAAGTCTA CAGATAGTCT TCATGCTGAA GTAAAGTTAC TGCGCCAGAA GTACCAGAAG
    ACCAACGAAA TTATCTCAAA AATGTTAAAT AGCCAAGACA CTGCTGTCCA TAGAATAATA
    GAGTTTGAAG ATCAGCTAGC CCGTCTATCA TCGGTTCGAA ATAACTCTAT AAAACAATCA
    ACAACGTTTC AAGTCAAAAA GTCATCTCAA AAATCAACTA TTCAAAACCT GGAAGAAAAG
    GTGTCTTATT TACAGCAATT TATGGATAAA AATAATGCTA CATTAACAGA CCTTGAATTT
    CAATGCTCAG ATTTATCATC TTCTATAGAT ATACTTTCCA AGCAAGATGA AGAGCATGAA
    AAAGAGAAGC GAAAGTTAAA GGATACTGGG GTATCAACGT CAGCTGAAGA GTTAAAAACT
    TTTAGGGCCA TGTGTAAGTG TTCAGTTTGT AACTTCGAGC GCTGGAAAGA TCGAATTATT
    TCTCTCTGTG GTCATGGCTT TTGTTACCAA TGCATACAAA AACGGATTGA AACACGGCAA
    CGCAGATGCC CGATTTGCGG TCGTGGATTT GGTGCTTCTG ATGTTATTCC GATACATCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587845.1
    CDS240..2282
    Translation

    Target ORF information:

    RefSeq Version NM_001022838.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ubiquitin-protein ligase E3 Brl2 (brl2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022838.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTATCAGA ATGGTAAACC AGATGCCCCC ACAATCTTAG GCCAGAAAAG AGAACTTGAA 
    GATGTAGAAA TACAAGATGA TGATATTCAG GAAGTCTCTA AAGAAGACTT ATTAAAGGAT
    GTAAGAGTGA GGAGCATTCA ATTTGATGAG TTAGAAAGTA AAATAGAGGG ACTTCAAAAT
    TTAGCAGAAG AGAAATTAAA AGTTTTAGCT ACTTTGGTCT CTTGGTGGCC TGAAATACTT
    CAGCAGTTTA GCGTTGTGTT TCAAGGCAAT GAGCTGAAGG ACTTCGAGAG TGAAGGTGTT
    TTTTCGATTT TGGAGAAATT CCCGGAGCTT TCATACTTCA ATGACGCTGT GAAAAATAAT
    AAAACTAAAG CCTTGAGCAT CATTCAAAAA CTCTTGTCTA CTGTTGACTC AAGCACAAAT
    TCGGTCTCCA GAGATCCTTT TTCAGTTTTA TCAATTGATG ACTCTGCCCT TACTGAGAAA
    TTAAACACCA TTAATTTGGA TATTGACAAG ATTTTAGATG AATTGGATAC CACTCGGTCT
    CAGCTTCATT CAATTATAAA ACTACCAGAT CGCTCGTCTA GTTTTACTTT GCAATGCATT
    AATGAATCAG TGAGACCCCA GTCTACTAAA GTGAAAGAGG AAGCAACCAC CTCCTCTAAG
    GGGAAAGATG AAGAAAAGAA AGTTAGCACG GTTGAACAAC GTACTCAACT GCAACAATTA
    TCCAGGCTTC AAGACCAACA AAATGGTTTA ATGGAGTCTC GCTCACAAAG CCTTAAAATT
    TTGGATTCGA ACGTTAATGA AATGGATAAA TTAATTATGG AACGAGAAAA CGCATTAAAC
    AACGTCGAAA CTACAAACTT GAAAAAATAC TCTTCCTTTT TAGCACTTAA AGAAGCTGTT
    TCTATGACAT CTGAGCAGTT ACGCGTATTA GAGCACTTAT TGTCCGAATG TTCTCACGAA
    ATAAATGTAT TGTCGCAACA GTCAAAGAAT TTTAACGGTG TTTTTGAATC TTCCTATCAG
    CCGTTAATAA ACGATCTTGA TCATCAAATA TCTGTTATGC AAAACGATGA AAAAAGAATT
    AATAACGCAA AGACTGAATT AAGCTTATCC CTTGAAAAGA AGTTGGAAGC TAAAAAGCAA
    AAAGAGAAGG TCTACAAAGA CAAGTTAGAT GAACTTGCGA ATTTAGAGAC AATGGTATTG
    GAAAAGAAAA AAGCGGTGGC TACCCGTGAA GCTGCAAACA AAATCCGGCT CGTAGACCTT
    AACGATTTGG AGTTACAAAA AGATCTGTCA ACTTACCTTT CTAAAGAGTT GGCTTCTACT
    GAAAAAGCAT TTAGGTTGGT AAAACAGCAA ACTGTGAAAA GTAGCCACTC CCATTATCAA
    GAATTGATAA CCAAATTTAG TGTCGAAAAG GAAAAGGCGG AGCAAAAATA TTTTCTGACC
    ATGAAGTCTA CAGATAGTCT TCATGCTGAA GTAAAGTTAC TGCGCCAGAA GTACCAGAAG
    ACCAACGAAA TTATCTCAAA AATGTTAAAT AGCCAAGACA CTGCTGTCCA TAGAATAATA
    GAGTTTGAAG ATCAGCTAGC CCGTCTATCA TCGGTTCGAA ATAACTCTAT AAAACAATCA
    ACAACGTTTC AAGTCAAAAA GTCATCTCAA AAATCAACTA TTCAAAACCT GGAAGAAAAG
    GTGTCTTATT TACAGCAATT TATGGATAAA AATAATGCTA CATTAACAGA CCTTGAATTT
    CAATGCTCAG ATTTATCATC TTCTATAGAT ATACTTTCCA AGCAAGATGA AGAGCATGAA
    AAAGAGAAGC GAAAGTTAAA GGATACTGGG GTATCAACGT CAGCTGAAGA GTTAAAAACT
    TTTAGGGCCA TGTGTAAGTG TTCAGTTTGT AACTTCGAGC GCTGGAAAGA TCGAATTATT
    TCTCTCTGTG GTCATGGCTT TTGTTACCAA TGCATACAAA AACGGATTGA AACACGGCAA
    CGCAGATGCC CGATTTGCGG TCGTGGATTT GGTGCTTCTG ATGTTATTCC GATACATCTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L