spc7 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	spc7
Entrez Gene ID	2539155
Full Name	kinetochore protein Spc7
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022949.2	NP_587958.1	kinetochore protein Spc7

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit spindle assembly checkpoint proteins and promote chromosome alignment.
Title: MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.

Spc7 interacts genetically with the Mal2-containing Sim4 complex.
Title: The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.

spc7 can suppress the mal3 mutant phenotype. Spc7, an essential component of the kinetochore that associates with the central centromere region, interacts with the microtubule plus-end-associated protein Mal3 at the microtubule-kinetochore interface.
Title: The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.

The following spc7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spc7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99191	NM_001022949.2 Latest version!	Schizosaccharomyces pombe kinetochore protein Spc7 (spc7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spc7 ( NM_001022949.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587958.1 Schizosaccharomyces pombe kinetochore protein Spc7 (spc7), mRNA.

Price & Availability↑

CloneID	OSc99191
Clone ID Related Accession (Same CDS sequence)	NM_001022949.2
Accession Version	NM_001022949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	kinetochore protein Spc7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022949.1.

ATGCCAACAT CGCCTCGTCG CAATAGTATA GCGACGACCG ATAATGTTAT AGGAAGAAAC 
AAAAGTAGGA AGCGTCCACA CTCGCTTGGA GGACCTGGTG CGCTTCAAGA ACTTAAAGAA 
CACACAAATC CAGCAAAAGG CATTCTAAAG AGTTATTCAT CCTTTTCCGT AGATCCCGCT 
TTCACAGGAG ATGAAGACTT TAATGATATA CACACACAAA TAAATAACAC GGTCATTGGA 
ATCTCTAGTA ATGTTATGGC TGCGTCAAAG AGACTTCAAA TGGAAGACCT ACAGCAATTG 
TCGCGTGAGA CGTCTAGAAA AAGTTTGAAC AGAAGAGTTT CCTTTGCTAG TCATGCACGT 
GTACGTTGGT ATCCTAAAGA TCACCAGAGT GACTCGGAAA AGTCGACATC TAATCACAGC 
ACACCTGAGC GAACTTTTGC ATCTGATGCT AAAAACCATT CCCCTAAAGG TCCAACAACG 
ACAAGTTTTT CGAGAAACGA AACACAATCA TCTCCCCATA GTCATAGTGC CTCGATTATT 
TCAGACGGTA GTGATATGGA CATTGCGTCC CCTATTCGGT CAACCGAATC AGATATGGTC 
TCTGAGGCAT TGAATGCTGG CCATCCGCCT CCTTCTCTGT ATCCGGAGAA TGATGATTTA 
AGTATCCAGA ATCCAACAAA AGCCTTACCT GAAGCAGAAA AGGCACTTGA TGTTCACGAT 
GCCACGAGAG AGCAGGTTAA TGATAGAGAG GAAACTAACA TGGATTTGAC TATCCAATTC 
CAAGAAGCAG ATTCGTTTTT ATCACATTCC GAATCAATTA AAGGTCTTAG CTCTAGCGAG 
CAAGGTACTG TTTATAGTTT AAAAGCTTCT CATGACCCTT CAAACCAAAC TCAACTTTCA 
TCCCCTAACA AATCATCTAG TCCAACTAGT ATTGAAATTT CGGATTTCTC CAAAAATAAC 
GAAAATCACG ATCAGTCGGA AAATAAAGAA GAAGAAGAAG ATATGATGCT AACTCGACCT 
ATTGAAATTC CGCAACACTT TTCCCCCATC GCTCGCCCTT TAACTTCCCA AGAAGCTATT 
GTTGACATGG ATATTACCTC TAATAACATT AATTTATCTC CAGTGTCTCA TTTTTCCAAT 
GGCTTAGATT TACAAAACTT AGAAGAAGCG CCTATGAACC TTACCCGACC AATTAATGCT 
AATCCTCACT TAACTAATCA TTCTCCGAAC GATTTAACTA ATGGAGAAGA AGAAATGGAT 
ACCACCTCCG CCTTTAATAT CGAAAACTCA CATTTAACTC TCCTTTCCCC CATTCGTCCA 
TCGTCACGAT CCATGGAAGA GCAAATAATG GACTTGACAC AACCCATTAG CTCTACAAAT 
GCACCCACCC ATTTGAATGA AGATGATTTA AATCAATTTA CCTCAAATAT ATCCTCTTCT 
TCAAAACCGA GAAAGGATAA TAACAAGACT GCCAATTCTT CAAAGCCCAT TCCCGACAGT 
GAGGACTTTA TGGACATTAC TAGACCTTTC AATATATTAT CCCCTTCTAA AGAAGCACTT 
TCCGAAGAGC AACCAATGGA ATTAACCTCA ACAGTTTTTC CTTGTGAGAA TTCTACATCT 
CATCTTGAGG TGGAAGAAGC TGCTATGGAC GAAACAGTGG CTTTTCAAAT CCGTGGAAAC 
AATGTTGAGT TACCTTCAGC AGACAAGGAA AATGCCGAGA GAGAAGAAAT ACCCTCATAT 
AGTGATAAGT CAGAAAATTT CAATACTACC TCCTTTACCA ACCATGAACG GTCACCAAAT 
GGGAACAATA ATCTAAAATT TTCGAAAGAT CCTAACTCTT CTTCTCCAAG TAGACATGTG 
GTAGCGACTC CTACTGATAA GCTTGGTACT CGGAAAAGAC GCTTAAGATA TAGCACTTCA 
AGTTTTGATC AAAGTACCTT GCGTCGCAAT CGTCTTGCAA CAATCCGTAA TGCTCGAAAA 
TCAATTTCAA CTTTGAATGA CAGAGAACTT CTACCGGTTA ACTTTTTCGA AAAAAAAGTA 
AATTCTGGTC TCTATAAATC GGTTGAGCGA TCCGAAAATT ATCGTCTAGG AGCTACTCCA 
CTTACCGCAG AGAAGCCATT CACCACAGAA AAGCCGCTTT CAAGTCTTCC TGAAGAAGTT 
TCAAGGCAAC CCACTGATGA CAAAGGCGAA CAAGTGTCTA ATGCAGATGT TGATTCTGGA 
TTGAGTAAAA CAGAGCGACT AACAATTCAA CAGACTAACG AAATTAAGCA CGTTCCAACT 
AATACGACTT CTTCTGTTAA GCTTCCTCAA CAACCTTCGA ATGAGGATGA AAAAGAGCGT 
ATAACAACTG CAGATTACGC CGATTCAACT AGTTTGGAGC GATTGGAATC TCAGGAACCC 
AACCGGAATG AACTTGTTCA AGTTGGTTCG TCAAATGCAG GTAATACAAC TTCCGTGGGC 
ATGAATGAAC ATGAGAAGTC ACCCGTCAAA TTATCAAAAG GTGTGAGCAA TGTTGATACA 
TCGTTGGGCG CTTCGACTAT TAACACGAAC ATTTTGAATC AAGACTCTGG TCCAAATGAA 
GAAATACCTG TTGGTAATGA GCCAGAATTT GATACGATGC CAACTCTACC AAACGTCGAG 
CCGATAAGCC TATCAGATTT TCTTAAAATG ACCGGAATTG AATTTTTGGA TAATCTTACT 
ATTGCTAAAA GACGAGAAAC CCTATTGCCA AATGCTGAAG AAAATAAGAA ATGCAGTATC 
CAAGAACTTT TGGAATCTTT TTACATACAA TTCCCTTTGT TAGAGTTATA CAAATTTAGT 
TGTCAGCAAT TACAGGATTA TATTGCCGAA GGGAAGGATT TTGTTACTAA AATTGAAGAG 
GAAACTTTGA AAGAGAACCC GTTACTATTC TATGAGTATA GGAAAGCATC CAGTGATATG 
AGGGTTTTAA TGGATTCACA ATTTTTAATG ATGAAAACTT TTGCTAGACT TCAAGCAAAA 
GGTGATTGGT ACGAATGGCG TGAGGGTTTG ATGCAAGGAA TCAAACATGA ATTAAATTTA 
AATTTAACTG GGATGCAAAG AAGCTTGACC CATTTAATGG ATGTCGCGAA TGTTATTCAT 
CCGTATGCGC AGGAAATTCA AGAGAGATAC AATGGTAGTA TTACTACAGT ACAGACATTA 
AAGAAGCAGA AGGAATTTGC AAATCAGTAT GACTCAACTC TTCTAGCGCA GGCGCAAGAA 
AAGTTAGAGA AACTCAAGGT TGAGGTTGAG AGGAGACGTC GGCTTCTGAG TGAAAAAGAA 
GAAAGAAGAA AGGAGTTGGC AATAAAGATA GAACAAGTGA CAAACTCTTG CTCTGATTTG 
GAATTACGTA CCAACGCTGA ACAAGATTTT TATGCGAAAA ACCAAGATTT TGAATTTGAC 
GAAATTAAGC GATACGAGGA GCAGTTGTTA AATCTTAAAA ATGAACTTGG CTGGACCATA 
GTTTCTTTGA CCGCGGGCGG AATTAAATTA GCTACAAATA ACACTGCTCT TTCTCCTTAT 
TCAGCCGAGG TTACGGTTGA GATTTTGAGG CAAAATTTCC AAGTTAACGT TGATATTGCC 
TGTAAGTTCC CTAACGAAAG TAATGCTTGC AGCTCAAATG TGCTCGAGCA TGTTGCATCG 
TCGTTTTCTA AATGGCATTC CAAGGTATTT TCTAGGAACT TACGTCTTCT GAAGAAGTAT 
CTTAACGATG TTTCAATTTG TTGGGAACAA ATTGTTTATC TTGTCCAAGA TTTCCAGCGA 
TTATGGTACC ATTGGCCCTT TTTGTCAGTT GAAAATGATG ATAAGTCTAT TATCATAAAT 
GTTGAGCTAT ACCTGCGTTC GGTATCATCT AAGGTAAAGG TTGTTTTCGG TCTCCCAATT 
GATACTATCT ACCAAACTAC TGAAGTAGGA AAGTTTTATG CTTCTACATC AGTCGCTGTG 
AAACAGATGT ATGCGGAAAG TGAAGGAGAC TCTTATGTTA GTGAGGTTTT GAATACTCTT 
TCGGAAGTGG TGCATTGTAC ATCAACTTAT GCCCTTTCGT CTGCTTGTCT TACTGTTTGG 
AACAAATACA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_587958.1
CDS	550..4644
Translation	MPTSPRRNSIATTDNVIGRNKSRKRPHSLGGPGALQELKEHTNPAKGILKSYSSFSVDPAFTGDEDFNDIHTQINNTVIGISSNVMAASKRLQMEDLQQLSRETSRKSLNRRVSFASHARVRWYPKDHQSDSEKSTSNHSTPERTFASDAKNHSPKGPTTTSFSRNETQSSPHSHSASIISDGSDMDIASPIRSTESDMVSEALNAGHPPPSLYPENDDLSIQNPTKALPEAEKALDVHDATREQVNDREETNMDLTIQFQEADSFLSHSESIKGLSSSEQGTVYSLKASHDPSNQTQLSSPNKSSSPTSIEISDFSKNNENHDQSENKEEEEDMMLTRPIEIPQHFSPIARPLTSQEAIVDMDITSNNINLSPVSHFSNGLDLQNLEEAPMNLTRPINANPHLTNHSPNDLTNGEEEMDTTSAFNIENSHLTLLSPIRPSSRSMEEQIMDLTQPISSTNAPTHLNEDDLNQFTSNISSSSKPRKDNNKTANSSKPIPDSEDFMDITRPFNILSPSKEALSEEQPMELTSTVFPCENSTSHLEVEEAAMDETVAFQIRGNNVELPSADKENAEREEIPSYSDKSENFNTTSFTNHERSPNGNNNLKFSKDPNSSSPSRHVVATPTDKLGTRKRRLRYSTSSFDQSTLRRNRLATIRNARKSISTLNDRELLPVNFFEKKVNSGLYKSVERSENYRLGATPLTAEKPFTTEKPLSSLPEEVSRQPTDDKGEQVSNADVDSGLSKTERLTIQQTNEIKHVPTNTTSSVKLPQQPSNEDEKERITTADYADSTSLERLESQEPNRNELVQVGSSNAGNTTSVGMNEHEKSPVKLSKGVSNVDTSLGASTINTNILNQDSGPNEEIPVGNEPEFDTMPTLPNVEPISLSDFLKMTGIEFLDNLTIAKRRETLLPNAEENKKCSIQELLESFYIQFPLLELYKFSCQQLQDYIAEGKDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQAKGDWYEWREGLMQGIKHELNLNLTGMQRSLTHLMDVANVIHPYAQEIQERYNGSITTVQTLKKQKEFANQYDSTLLAQAQEKLEKLKVEVERRRRLLSEKEERRKELAIKIEQVTNSCSDLELRTNAEQDFYAKNQDFEFDEIKRYEEQLLNLKNELGWTIVSLTAGGIKLATNNTALSPYSAEVTVEILRQNFQVNVDIACKFPNESNACSSNVLEHVASSFSKWHSKVFSRNLRLLKKYLNDVSICWEQIVYLVQDFQRLWYHWPFLSVENDDKSIIINVELYLRSVSSKVKVVFGLPIDTIYQTTEVGKFYASTSVAVKQMYAESEGDSYVSEVLNTLSEVVHCTSTYALSSACLTVWNKYS

Target ORF information:

RefSeq Version	NM_001022949.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe kinetochore protein Spc7 (spc7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022949.2

ORF Insert Sequence:

ATGCCAACAT CGCCTCGTCG CAATAGTATA GCGACGACCG ATAATGTTAT AGGAAGAAAC 
AAAAGTAGGA AGCGTCCACA CTCGCTTGGA GGACCTGGTG CGCTTCAAGA ACTTAAAGAA 
CACACAAATC CAGCAAAAGG CATTCTAAAG AGTTATTCAT CCTTTTCCGT AGATCCCGCT 
TTCACAGGAG ATGAAGACTT TAATGATATA CACACACAAA TAAATAACAC GGTCATTGGA 
ATCTCTAGTA ATGTTATGGC TGCGTCAAAG AGACTTCAAA TGGAAGACCT ACAGCAATTG 
TCGCGTGAGA CGTCTAGAAA AAGTTTGAAC AGAAGAGTTT CCTTTGCTAG TCATGCACGT 
GTACGTTGGT ATCCTAAAGA TCACCAGAGT GACTCGGAAA AGTCGACATC TAATCACAGC 
ACACCTGAGC GAACTTTTGC ATCTGATGCT AAAAACCATT CCCCTAAAGG TCCAACAACG 
ACAAGTTTTT CGAGAAACGA AACACAATCA TCTCCCCATA GTCATAGTGC CTCGATTATT 
TCAGACGGTA GTGATATGGA CATTGCGTCC CCTATTCGGT CAACCGAATC AGATATGGTC 
TCTGAGGCAT TGAATGCTGG CCATCCGCCT CCTTCTCTGT ATCCGGAGAA TGATGATTTA 
AGTATCCAGA ATCCAACAAA AGCCTTACCT GAAGCAGAAA AGGCACTTGA TGTTCACGAT 
GCCACGAGAG AGCAGGTTAA TGATAGAGAG GAAACTAACA TGGATTTGAC TATCCAATTC 
CAAGAAGCAG ATTCGTTTTT ATCACATTCC GAATCAATTA AAGGTCTTAG CTCTAGCGAG 
CAAGGTACTG TTTATAGTTT AAAAGCTTCT CATGACCCTT CAAACCAAAC TCAACTTTCA 
TCCCCTAACA AATCATCTAG TCCAACTAGT ATTGAAATTT CGGATTTCTC CAAAAATAAC 
GAAAATCACG ATCAGTCGGA AAATAAAGAA GAAGAAGAAG ATATGATGCT AACTCGACCT 
ATTGAAATTC CGCAACACTT TTCCCCCATC GCTCGCCCTT TAACTTCCCA AGAAGCTATT 
GTTGACATGG ATATTACCTC TAATAACATT AATTTATCTC CAGTGTCTCA TTTTTCCAAT 
GGCTTAGATT TACAAAACTT AGAAGAAGCG CCTATGAACC TTACCCGACC AATTAATGCT 
AATCCTCACT TAACTAATCA TTCTCCGAAC GATTTAACTA ATGGAGAAGA AGAAATGGAT 
ACCACCTCCG CCTTTAATAT CGAAAACTCA CATTTAACTC TCCTTTCCCC CATTCGTCCA 
TCGTCACGAT CCATGGAAGA GCAAATAATG GACTTGACAC AACCCATTAG CTCTACAAAT 
GCACCCACCC ATTTGAATGA AGATGATTTA AATCAATTTA CCTCAAATAT ATCCTCTTCT 
TCAAAACCGA GAAAGGATAA TAACAAGACT GCCAATTCTT CAAAGCCCAT TCCCGACAGT 
GAGGACTTTA TGGACATTAC TAGACCTTTC AATATATTAT CCCCTTCTAA AGAAGCACTT 
TCCGAAGAGC AACCAATGGA ATTAACCTCA ACAGTTTTTC CTTGTGAGAA TTCTACATCT 
CATCTTGAGG TGGAAGAAGC TGCTATGGAC GAAACAGTGG CTTTTCAAAT CCGTGGAAAC 
AATGTTGAGT TACCTTCAGC AGACAAGGAA AATGCCGAGA GAGAAGAAAT ACCCTCATAT 
AGTGATAAGT CAGAAAATTT CAATACTACC TCCTTTACCA ACCATGAACG GTCACCAAAT 
GGGAACAATA ATCTAAAATT TTCGAAAGAT CCTAACTCTT CTTCTCCAAG TAGACATGTG 
GTAGCGACTC CTACTGATAA GCTTGGTACT CGGAAAAGAC GCTTAAGATA TAGCACTTCA 
AGTTTTGATC AAAGTACCTT GCGTCGCAAT CGTCTTGCAA CAATCCGTAA TGCTCGAAAA 
TCAATTTCAA CTTTGAATGA CAGAGAACTT CTACCGGTTA ACTTTTTCGA AAAAAAAGTA 
AATTCTGGTC TCTATAAATC GGTTGAGCGA TCCGAAAATT ATCGTCTAGG AGCTACTCCA 
CTTACCGCAG AGAAGCCATT CACCACAGAA AAGCCGCTTT CAAGTCTTCC TGAAGAAGTT 
TCAAGGCAAC CCACTGATGA CAAAGGCGAA CAAGTGTCTA ATGCAGATGT TGATTCTGGA 
TTGAGTAAAA CAGAGCGACT AACAATTCAA CAGACTAACG AAATTAAGCA CGTTCCAACT 
AATACGACTT CTTCTGTTAA GCTTCCTCAA CAACCTTCGA ATGAGGATGA AAAAGAGCGT 
ATAACAACTG CAGATTACGC CGATTCAACT AGTTTGGAGC GATTGGAATC TCAGGAACCC 
AACCGGAATG AACTTGTTCA AGTTGGTTCG TCAAATGCAG GTAATACAAC TTCCGTGGGC 
ATGAATGAAC ATGAGAAGTC ACCCGTCAAA TTATCAAAAG GTGTGAGCAA TGTTGATACA 
TCGTTGGGCG CTTCGACTAT TAACACGAAC ATTTTGAATC AAGACTCTGG TCCAAATGAA 
GAAATACCTG TTGGTAATGA GCCAGAATTT GATACGATGC CAACTCTACC AAACGTCGAG 
CCGATAAGCC TATCAGATTT TCTTAAAATG ACCGGAATTG AATTTTTGGA TAATCTTACT 
ATTGCTAAAA GACGAGAAAC CCTATTGCCA AATGCTGAAG AAAATAAGAA ATGCAGTATC 
CAAGAACTTT TGGAATCTTT TTACATACAA TTCCCTTTGT TAGAGTTATA CAAATTTAGT 
TGTCAGCAAT TACAGGATTA TATTGCCGAA GGGAAGGATT TTGTTACTAA AATTGAAGAG 
GAAACTTTGA AAGAGAACCC GTTACTATTC TATGAGTATA GGAAAGCATC CAGTGATATG 
AGGGTTTTAA TGGATTCACA ATTTTTAATG ATGAAAACTT TTGCTAGACT TCAAGCAAAA 
GGTGATTGGT ACGAATGGCG TGAGGGTTTG ATGCAAGGAA TCAAACATGA ATTAAATTTA 
AATTTAACTG GGATGCAAAG AAGCTTGACC CATTTAATGG ATGTCGCGAA TGTTATTCAT 
CCGTATGCGC AGGAAATTCA AGAGAGATAC AATGGTAGTA TTACTACAGT ACAGACATTA 
AAGAAGCAGA AGGAATTTGC AAATCAGTAT GACTCAACTC TTCTAGCGCA GGCGCAAGAA 
AAGTTAGAGA AACTCAAGGT TGAGGTTGAG AGGAGACGTC GGCTTCTGAG TGAAAAAGAA 
GAAAGAAGAA AGGAGTTGGC AATAAAGATA GAACAAGTGA CAAACTCTTG CTCTGATTTG 
GAATTACGTA CCAACGCTGA ACAAGATTTT TATGCGAAAA ACCAAGATTT TGAATTTGAC 
GAAATTAAGC GATACGAGGA GCAGTTGTTA AATCTTAAAA ATGAACTTGG CTGGACCATA 
GTTTCTTTGA CCGCGGGCGG AATTAAATTA GCTACAAATA ACACTGCTCT TTCTCCTTAT 
TCAGCCGAGG TTACGGTTGA GATTTTGAGG CAAAATTTCC AAGTTAACGT TGATATTGCC 
TGTAAGTTCC CTAACGAAAG TAATGCTTGC AGCTCAAATG TGCTCGAGCA TGTTGCATCG 
TCGTTTTCTA AATGGCATTC CAAGGTATTT TCTAGGAACT TACGTCTTCT GAAGAAGTAT 
CTTAACGATG TTTCAATTTG TTGGGAACAA ATTGTTTATC TTGTCCAAGA TTTCCAGCGA 
TTATGGTACC ATTGGCCCTT TTTGTCAGTT GAAAATGATG ATAAGTCTAT TATCATAAAT 
GTTGAGCTAT ACCTGCGTTC GGTATCATCT AAGGTAAAGG TTGTTTTCGG TCTCCCAATT 
GATACTATCT ACCAAACTAC TGAAGTAGGA AAGTTTTATG CTTCTACATC AGTCGCTGTG 
AAACAGATGT ATGCGGAAAG TGAAGGAGAC TCTTATGTTA GTGAGGTTTT GAATACTCTT 
TCGGAAGTGG TGCATTGTAC ATCAACTTAT GCCCTTTCGT CTGCTTGTCT TACTGTTTGG 
AACAAATACA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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spc7 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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