SPAC31G5.19 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC31G5.19
Entrez Gene ID	2543084
Full Name	ATPase with bromodomain protein (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356095.1	NP_001342853.1	putative bromodomain-containing ATPase
NM_001019446.2	NP_594020.1	ATPase with bromodomain protein (predicted)

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC31G5.19	NP_594020.1
Eremothecium gossypii	AGOS_ABR139W	NP_983086.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC31G5.19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC31G5.19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102516	NM_001356095.1 Latest version!	Schizosaccharomyces pombe putative bromodomain-containing ATPase (SPAC31G5.19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OSj04258	NM_001019446.2 Latest version!	Schizosaccharomyces pombe 972h- ATPase with bromodomain protein (predicted) (SPAC31G5.19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC31G5.19 ( NM_001356095.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342853.1 Schizosaccharomyces pombe putative bromodomain-containing ATPase (SPAC31G5.19), mRNA.

Price & Availability↑

CloneID	OSc102516
Clone ID Related Accession (Same CDS sequence)	NM_001356095.1
Accession Version	NM_001356095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative bromodomain-containing ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGAAGGAAG AAGCAAGTGA ACATGGTGGA TCTGCGGATG AAACTCAAGA GTTGTCACCA 
GTGTCCGATT CTTCTGATGA GATGCCCAAC AATGCTAAGC GTCGTCGCCG TTCCCAGTCC 
ATGATTGCTA ACAAACGCAT TCATCAAGCT TTCCAGGAAG ATGAAGGTGA CGAGGATTGG 
GAAGAAGAGG AACATAAGCC CAAAGCGAAA CGGCGATATA ATACTCGGTC AAATGAAAGC 
TTTTCAGAGG GTGATGATGA ACCTTTTGAA GTATCAGAGT CCTCGGCTTT GGAAGATGAA 
CTATCAGATT CTGAAGATTC TTTTATACGA TCAGTCCGTT CTAAACCCAA GTACAAACCT 
GGAACTCGTC GCTCAACACG CCTTAGAAAT CGTAGATCAC AGGATGAAGA AGAATCCGAA 
GAAGAGCATA GACCTATACT GAGAGAACGG ACAAGTCGTA TAAATTACAG CGTTCCGTTG 
GCTTTCCCTC CAGTCGATGA AATGGATGGT GATCCTAGCT CTCAAGTCAA TCAAAGCAGG 
AGTAGGAAGA CGCATTCTGA ACTTGCTATT ACTAAGCTTC TCCGACAACA AGTTAGTAGT 
TTTATGCCAT ATATTGATTC TAGTGGGTCT GAATCTGAAA GCGACAATAC AAGAATTAAA 
AAGTCGTCTG CAAAAACCAT AAAAGCTTTG ACTGACCCAG CTAATTCCGG TGGGCCGCCT 
GATTTTGGCC GTATCAGGGA GAAAAGCGAT CTTGCCGACT CAGATCCTCT GGGAGTCGAC 
TCTTCTTTAT CCTTCGAATC CGTCGGTGGA CTGGACAATT ACATTAATCA ATTAAAAGAA 
ATGGTCATGC TTCCTTTGTT ATACCCTGAA ATATTTCAGC GTTTTAACAT GCAACCACCA 
AGGGGAGTAC TTTTTCATGG TCCTCCTGGT ACAGGAAAAA CTTTAATGGC TCGAGCTCTT 
GCTGCAGCTT GTTCTTCCGA AAACAAAAAA GTATCTTTCT ATATGAGAAA AGGCGCTGAT 
TGCCTCAGTA AATGGGTTGG CGAGGCTGAG CGTCAGCTTC GATTACTTTT CGAAGAAGCA 
AAAAGTACTC AACCAAGTAT AATTTTCTTT GATGAAATAG ATGGTCTGGC TCCTGTGAGA 
TCGAGTAAAC AAGAGCAAAT TCACGCATCT ATTGTTTCTA CATTATTGGC GTTAATGGAT 
GGCATGGAAA GCCGAGGCCA GGTCATTATA ATAGGTGCAA CAAACCGACC TGACGCAGTT 
GATCCAGCTC TCCGTCGTCC TGGTCGGTTT GACAGAGAAT TTTATTTTCC TTTGCCTGAT 
CGTGATGCCA GGAAAAAGAT AATAGAGATT CACACGCGAA ATTGGGATCC ACCAGTTCCT 
GAATGGCTAT GTTCGATGCT TGCCGAAAAG TCAAAAGGCT ATGGTGGTGC AGATTTGCGT 
GCTCTATGCA CGGAGGCTGC TCTCAACTCT ATCAAACGTA CCTATCCTCA ATTATATAGG 
AGTACAAAGC GTCTTCAAAT TGATCCTAAA ACCATAAAAG TGAAGGTAAA GGACTTTGTA 
ATGTCGATGA AACGCATGAT CCCTTCTTCT GAGCGCTCAT CAATATCTCC AAGTAAGCCT 
CTATCACCGG AATTGAAACC ACTACTCAAC GAAGCTTTTC AAGATATTGA GAAGACTTTA 
CAGAAATTAA TGCCTGTTGC CTCTAAATTA AATCCTCTTG AGGAAGTAAT GTATGACGAT 
CCAAAAGAAA ATGACTTTGA GTACCAACAG CGCTTGGAGA CTTTTGAAAC TCTAAGAATT 
TATAAGCCTA GATTCCTTAT TTGTGGTAGA AAGGGACTGG GACAAACCGC GCTTGGTCCA 
GCCATTTTGC AGCAATATGA AGGAGTGCAT GTTCAATCAT TTGACATGTC TACTCTACTT 
CAAGACTCCA CTCAAAGTAT CGAGACCTCT ATAATTCATC TCTTTTTAGA AGTAAGGCGT 
CACACTCCAA GTATTATCTA TATTCCAGAT ATAGATAATT GGTTGAATGT CTTACCTTTG 
ACAGCTATTA CGACCTTTTC TTCTATGCTT GAGAGACTTG ATTTTTCGGA TCAAATACTC 
TTTCTTGCCT TATCTTCAAG TCCGTTAAGT GAATTGCATC CTCAATTAAG AGAATGGTTC 
TCTTCAAAGC AATCCGTTTA CTCATTACAG TATCCGACTC GTGATAGCAT TATCGCATTT 
TTTCAGCCAA TTTTGGAACT AATTAAAGCC TCACCAACCG AACTTCCTGG TGGCATACCT 
CGAAAACGCA GAGTTTTACC AGAGTTACCT CTTGCTCCCG ATCCTCCACC ATTTACCAGT 
CAAAAAATTA CCCTAAAACA AACTAAGCAG GCCGACATGC GTCTTCTTAA CAAGCTAAAA 
ATAAAGCTGA ATGCCCTACT TGGAAGCCTT CGAGCAAGAT ATCGAAAGTT TAAGAAACCA 
TTGATTGATT TCAATGATAT ATACTGTGTA GATCCCGAAA CAGGTCATTC TTATCGTAGT 
CGCGAGGAAT GCCATTATGA GTTTGTTGAT GATGTAGTTA AGCAAATTGG AAGCGATCAA 
AAGTTTAGCA TGATGAGTTT AGAAGAGATT GAGAAGCGAA CTTGGGATAA TTGCTACTGT 
ACTCCGAAAC AGTTTGTACA TGATATTAAG CTCATTTTAC GTGATGCACT GCAACTGGAA 
GATTCAGAAA CTATCAAAAG AGCCCAGGAA ATGTATGCAA ACGTTCTCTT GGGTGTCGAA 
GATATGGAAG ACGATCAATT TTCCCAGAGA TGTGAAAGGA TGGCGTTACG TGAGGCTGAA 
CGAAGAAAAT TAAGGCACGG AAAGCTTCAA AAACATTTGG ATGAAACAAA AGCAGATATG 
CAGTTTACTT CTGAGAAGCC TTCTGTTGAC GAATCAATTA CTGAAGTTGA CGATGCCATA 
AAAGATGGAC CACCCGTACT AGCTGAAACC CTTACCAATT CACTGATGGA GGATGTTGGG 
CCGGAGAACG TTGACATGGA CATAGAAGAT AATGAAATTT TCACTAATCA GTCTACTATG 
TCTGTTCCTT CCATGTTAGT TGAGAATGAA GAGTCTCCCA AGCCTGACGA ATATATTGAT 
CAAAAAGACA AAGTACAATC TCCATTGTTA AATGGGAAGA GTCCAGTAGG CGTTCCATCA 
GAAGCCGCAT TACGTGTTTC TACTGATGTC TCTACGAATA TCTCTTCTAA TGGCCGTGCT 
GATATCCCTG TTGACACCCT CATCACGTCT CCTGCTGATG TTCCTAATAA TGCTCCTACT 
GATGCCCATA ATATTACATC CGCTGATGGT CATATTGAGA ATATCGAACA AGAGGTGGTA 
TTCCCTGATT TGGTGTTTGA TGAGGACAGA CTTACTCCAT TGAAGCAGTT GCTTATTGAT 
TCAACAACAG GCTTCACCGT TGACCAGCTT TTACATCTTC ATTCATTTTT GTACCAGATC 
ATTTGGAATA CGAAGTCCGA ATGGAATAGA AACTCTGTGG TTGATGAATG CGAGCGAGCA 
GTAAAAGAGT TTATGATTAA TGCACTACAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342853.1
CDS	1306..4878
Translation	MKEEASEHGGSADETQELSPVSDSSDEMPNNAKRRRRSQSMIANKRIHQAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSEGDDEPFEVSESSALEDELSDSEDSFIRSVRSKPKYKPGTRRSTRLRNRRSQDEEESEEEHRPILRERTSRINYSVPLAFPPVDEMDGDPSSQVNQSRSRKTHSELAITKLLRQQVSSFMPYIDSSGSESESDNTRIKKSSAKTIKALTDPANSGGPPDFGRIREKSDLADSDPLGVDSSLSFESVGGLDNYINQLKEMVMLPLLYPEIFQRFNMQPPRGVLFHGPPGTGKTLMARALAAACSSENKKVSFYMRKGADCLSKWVGEAERQLRLLFEEAKSTQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMESRGQVIIIGATNRPDAVDPALRRPGRFDREFYFPLPDRDARKKIIEIHTRNWDPPVPEWLCSMLAEKSKGYGGADLRALCTEAALNSIKRTYPQLYRSTKRLQIDPKTIKVKVKDFVMSMKRMIPSSERSSISPSKPLSPELKPLLNEAFQDIEKTLQKLMPVASKLNPLEEVMYDDPKENDFEYQQRLETFETLRIYKPRFLICGRKGLGQTALGPAILQQYEGVHVQSFDMSTLLQDSTQSIETSIIHLFLEVRRHTPSIIYIPDIDNWLNVLPLTAITTFSSMLERLDFSDQILFLALSSSPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPTELPGGIPRKRRVLPELPLAPDPPPFTSQKITLKQTKQADMRLLNKLKIKLNALLGSLRARYRKFKKPLIDFNDIYCVDPETGHSYRSREECHYEFVDDVVKQIGSDQKFSMMSLEEIEKRTWDNCYCTPKQFVHDIKLILRDALQLEDSETIKRAQEMYANVLLGVEDMEDDQFSQRCERMALREAERRKLRHGKLQKHLDETKADMQFTSEKPSVDESITEVDDAIKDGPPVLAETLTNSLMEDVGPENVDMDIEDNEIFTNQSTMSVPSMLVENEESPKPDEYIDQKDKVQSPLLNGKSPVGVPSEAALRVSTDVSTNISSNGRADIPVDTLITSPADVPNNAPTDAHNITSADGHIENIEQEVVFPDLVFDEDRLTPLKQLLIDSTTGFTVDQLLHLHSFLYQIIWNTKSEWNRNSVVDECERAVKEFMINALQ

Target ORF information:

RefSeq Version	NM_001356095.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative bromodomain-containing ATPase (SPAC31G5.19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356095.1

ORF Insert Sequence:

ATGAAGGAAG AAGCAAGTGA ACATGGTGGA TCTGCGGATG AAACTCAAGA GTTGTCACCA 
GTGTCCGATT CTTCTGATGA GATGCCCAAC AATGCTAAGC GTCGTCGCCG TTCCCAGTCC 
ATGATTGCTA ACAAACGCAT TCATCAAGCT TTCCAGGAAG ATGAAGGTGA CGAGGATTGG 
GAAGAAGAGG AACATAAGCC CAAAGCGAAA CGGCGATATA ATACTCGGTC AAATGAAAGC 
TTTTCAGAGG GTGATGATGA ACCTTTTGAA GTATCAGAGT CCTCGGCTTT GGAAGATGAA 
CTATCAGATT CTGAAGATTC TTTTATACGA TCAGTCCGTT CTAAACCCAA GTACAAACCT 
GGAACTCGTC GCTCAACACG CCTTAGAAAT CGTAGATCAC AGGATGAAGA AGAATCCGAA 
GAAGAGCATA GACCTATACT GAGAGAACGG ACAAGTCGTA TAAATTACAG CGTTCCGTTG 
GCTTTCCCTC CAGTCGATGA AATGGATGGT GATCCTAGCT CTCAAGTCAA TCAAAGCAGG 
AGTAGGAAGA CGCATTCTGA ACTTGCTATT ACTAAGCTTC TCCGACAACA AGTTAGTAGT 
TTTATGCCAT ATATTGATTC TAGTGGGTCT GAATCTGAAA GCGACAATAC AAGAATTAAA 
AAGTCGTCTG CAAAAACCAT AAAAGCTTTG ACTGACCCAG CTAATTCCGG TGGGCCGCCT 
GATTTTGGCC GTATCAGGGA GAAAAGCGAT CTTGCCGACT CAGATCCTCT GGGAGTCGAC 
TCTTCTTTAT CCTTCGAATC CGTCGGTGGA CTGGACAATT ACATTAATCA ATTAAAAGAA 
ATGGTCATGC TTCCTTTGTT ATACCCTGAA ATATTTCAGC GTTTTAACAT GCAACCACCA 
AGGGGAGTAC TTTTTCATGG TCCTCCTGGT ACAGGAAAAA CTTTAATGGC TCGAGCTCTT 
GCTGCAGCTT GTTCTTCCGA AAACAAAAAA GTATCTTTCT ATATGAGAAA AGGCGCTGAT 
TGCCTCAGTA AATGGGTTGG CGAGGCTGAG CGTCAGCTTC GATTACTTTT CGAAGAAGCA 
AAAAGTACTC AACCAAGTAT AATTTTCTTT GATGAAATAG ATGGTCTGGC TCCTGTGAGA 
TCGAGTAAAC AAGAGCAAAT TCACGCATCT ATTGTTTCTA CATTATTGGC GTTAATGGAT 
GGCATGGAAA GCCGAGGCCA GGTCATTATA ATAGGTGCAA CAAACCGACC TGACGCAGTT 
GATCCAGCTC TCCGTCGTCC TGGTCGGTTT GACAGAGAAT TTTATTTTCC TTTGCCTGAT 
CGTGATGCCA GGAAAAAGAT AATAGAGATT CACACGCGAA ATTGGGATCC ACCAGTTCCT 
GAATGGCTAT GTTCGATGCT TGCCGAAAAG TCAAAAGGCT ATGGTGGTGC AGATTTGCGT 
GCTCTATGCA CGGAGGCTGC TCTCAACTCT ATCAAACGTA CCTATCCTCA ATTATATAGG 
AGTACAAAGC GTCTTCAAAT TGATCCTAAA ACCATAAAAG TGAAGGTAAA GGACTTTGTA 
ATGTCGATGA AACGCATGAT CCCTTCTTCT GAGCGCTCAT CAATATCTCC AAGTAAGCCT 
CTATCACCGG AATTGAAACC ACTACTCAAC GAAGCTTTTC AAGATATTGA GAAGACTTTA 
CAGAAATTAA TGCCTGTTGC CTCTAAATTA AATCCTCTTG AGGAAGTAAT GTATGACGAT 
CCAAAAGAAA ATGACTTTGA GTACCAACAG CGCTTGGAGA CTTTTGAAAC TCTAAGAATT 
TATAAGCCTA GATTCCTTAT TTGTGGTAGA AAGGGACTGG GACAAACCGC GCTTGGTCCA 
GCCATTTTGC AGCAATATGA AGGAGTGCAT GTTCAATCAT TTGACATGTC TACTCTACTT 
CAAGACTCCA CTCAAAGTAT CGAGACCTCT ATAATTCATC TCTTTTTAGA AGTAAGGCGT 
CACACTCCAA GTATTATCTA TATTCCAGAT ATAGATAATT GGTTGAATGT CTTACCTTTG 
ACAGCTATTA CGACCTTTTC TTCTATGCTT GAGAGACTTG ATTTTTCGGA TCAAATACTC 
TTTCTTGCCT TATCTTCAAG TCCGTTAAGT GAATTGCATC CTCAATTAAG AGAATGGTTC 
TCTTCAAAGC AATCCGTTTA CTCATTACAG TATCCGACTC GTGATAGCAT TATCGCATTT 
TTTCAGCCAA TTTTGGAACT AATTAAAGCC TCACCAACCG AACTTCCTGG TGGCATACCT 
CGAAAACGCA GAGTTTTACC AGAGTTACCT CTTGCTCCCG ATCCTCCACC ATTTACCAGT 
CAAAAAATTA CCCTAAAACA AACTAAGCAG GCCGACATGC GTCTTCTTAA CAAGCTAAAA 
ATAAAGCTGA ATGCCCTACT TGGAAGCCTT CGAGCAAGAT ATCGAAAGTT TAAGAAACCA 
TTGATTGATT TCAATGATAT ATACTGTGTA GATCCCGAAA CAGGTCATTC TTATCGTAGT 
CGCGAGGAAT GCCATTATGA GTTTGTTGAT GATGTAGTTA AGCAAATTGG AAGCGATCAA 
AAGTTTAGCA TGATGAGTTT AGAAGAGATT GAGAAGCGAA CTTGGGATAA TTGCTACTGT 
ACTCCGAAAC AGTTTGTACA TGATATTAAG CTCATTTTAC GTGATGCACT GCAACTGGAA 
GATTCAGAAA CTATCAAAAG AGCCCAGGAA ATGTATGCAA ACGTTCTCTT GGGTGTCGAA 
GATATGGAAG ACGATCAATT TTCCCAGAGA TGTGAAAGGA TGGCGTTACG TGAGGCTGAA 
CGAAGAAAAT TAAGGCACGG AAAGCTTCAA AAACATTTGG ATGAAACAAA AGCAGATATG 
CAGTTTACTT CTGAGAAGCC TTCTGTTGAC GAATCAATTA CTGAAGTTGA CGATGCCATA 
AAAGATGGAC CACCCGTACT AGCTGAAACC CTTACCAATT CACTGATGGA GGATGTTGGG 
CCGGAGAACG TTGACATGGA CATAGAAGAT AATGAAATTT TCACTAATCA GTCTACTATG 
TCTGTTCCTT CCATGTTAGT TGAGAATGAA GAGTCTCCCA AGCCTGACGA ATATATTGAT 
CAAAAAGACA AAGTACAATC TCCATTGTTA AATGGGAAGA GTCCAGTAGG CGTTCCATCA 
GAAGCCGCAT TACGTGTTTC TACTGATGTC TCTACGAATA TCTCTTCTAA TGGCCGTGCT 
GATATCCCTG TTGACACCCT CATCACGTCT CCTGCTGATG TTCCTAATAA TGCTCCTACT 
GATGCCCATA ATATTACATC CGCTGATGGT CATATTGAGA ATATCGAACA AGAGGTGGTA 
TTCCCTGATT TGGTGTTTGA TGAGGACAGA CTTACTCCAT TGAAGCAGTT GCTTATTGAT 
TCAACAACAG GCTTCACCGT TGACCAGCTT TTACATCTTC ATTCATTTTT GTACCAGATC 
ATTTGGAATA CGAAGTCCGA ATGGAATAGA AACTCTGTGG TTGATGAATG CGAGCGAGCA 
GTAAAAGAGT TTATGATTAA TGCACTACAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAC31G5.19 ( NM_001019446.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_594020.1 Schizosaccharomyces pombe 972h- ATPase with bromodomain protein (predicted) (SPAC31G5.19), mRNA.

Price & Availability↑

CloneID	OSj04258
Clone ID Related Accession (Same CDS sequence)	NM_001019446.2
Accession Version	NM_001019446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	ATPase with bromodomain protein (predicted)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019446.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAAGGAAG AAGCAAGTGA ACATGGTGGA TCTGCGGATG AAACTCAAGA GTTGTCACCA 
GTGTCCGATT CTTCTGATGA GATGCCCAAC AATGCTAAGC GTCGTCGCCG TTCCCAGTCC 
ATGATTGCTA ACAAACGCAT TCATCAAGCT TTCCAGGAAG ATGAAGGTGA CGAGGATTGG 
GAAGAAGAGG AACATAAGCC CAAAGCGAAA CGGCGATATA ATACTCGGTC AAATGAAAGC 
TTTTCAGAGG GTGATGATGA ACCTTTTGAA GTATCAGAGT CCTCGGCTTT GGAAGATGAA 
CTATCAGATT CTGAAGATTC TTTTATACGA TCAGTCCGTT CTAAACCCAA GTACAAACCT 
GGAACTCGTC GCTCAACACG CCTTAGAAAT CGTAGATCAC AGGATGAAGA AGAATCCGAA 
GAAGAGCATA GACCTATACT GAGAGAACGG ACAAGTCGTA TAAATTACAG CGTTCCGTTG 
GCTTTCCCTC CAGTCGATGA AATGGATGGT GATCCTAGCT CTCAAGTCAA TCAAAGCAGG 
AGTAGGAAGA CGCATTCTGA ACTTGCTATT ACTAAGCTTC TCCGACAACA AGTTAGTAGT 
TTTATGCCAT ATATTGATTC TAGTGGGTCT GAATCTGAAA GCGACAATAC AAGAATTAAA 
AAGTCGTCTG CAAAAACCAT AAAAGCTTTG ACTGACCCAG CTAATTCCGG TGGGCCGCCT 
GATTTTGGCC GTATCAGGGA GAAAAGCGAT CTTGCCGACT CAGATCCTCT GGGAGTCGAC 
TCTTCTTTAT CCTTCGAATC CGTCGGTGGA CTGGACAATT ACATTAATCA ATTAAAAGAA 
ATGGTCATGC TTCCTTTGTT ATACCCTGAA ATATTTCAGC GTTTTAACAT GCAACCACCA 
AGGGGAGTAC TTTTTCATGG TCCTCCTGGT ACAGGAAAAA CTTTAATGGC TCGAGCTCTT 
GCTGCAGCTT GTTCTTCCGA AAACAAAAAA GTATCTTTCT ATATGAGAAA AGGCGCTGAT 
TGCCTCAGTA AATGGGTTGG CGAGGCTGAG CGTCAGCTTC GATTACTTTT CGAAGAAGCA 
AAAAGTACTC AACCAAGTAT AATTTTCTTT GATGAAATAG ATGGTCTGGC TCCTGTGAGA 
TCGAGTAAAC AAGAGCAAAT TCACGCATCT ATTGTTTCTA CATTATTGGC GTTAATGGAT 
GGCATGGAAA GCCGAGGCCA GGTCATTATA ATAGGTGCAA CAAACCGACC TGACGCAGTT 
GATCCAGCTC TCCGTCGTCC TGGTCGGTTT GACAGAGAAT TTTATTTTCC TTTGCCTGAT 
CGTGATGCCA GGAAAAAGAT AATAGAGATT CACACGCGAA ATTGGGATCC ACCAGTTCCT 
GAATGGCTAT GTTCGATGCT TGCCGAAAAG TCAAAAGGCT ATGGTGGTGC AGATTTGCGT 
GCTCTATGCA CGGAGGCTGC TCTCAACTCT ATCAAACGTA CCTATCCTCA ATTATATAGG 
AGTACAAAGC GTCTTCAAAT TGATCCTAAA ACCATAAAAG TGAAGGTAAA GGACTTTGTA 
ATGTCGATGA AACGCATGAT CCCTTCTTCT GAGCGCTCAT CAATATCTCC AAGTAAGCCT 
CTATCACCGG AATTGAAACC ACTACTCAAC GAAGCTTTTC AAGATATTGA GAAGACTTTA 
CAGAAATTAA TGCCTGTTGC CTCTAAATTA AATCCTCTTG AGGAAGTAAT GTATGACGAT 
CCAAAAGAAA ATGACTTTGA GTACCAACAG CGCTTGGAGA CTTTTGAAAC TCTAAGAATT 
TATAAGCCTA GATTCCTTAT TTGTGGTAGA AAGGGACTGG GACAAACCGC GCTTGGTCCA 
GCCATTTTGC AGCAATATGA AGGAGTGCAT GTTCAATCAT TTGACATGTC TACTCTACTT 
CAAGACTCCA CTCAAAGTAT CGAGACCTCT ATAATTCATC TCTTTTTAGA AGTAAGGCGT 
CACACTCCAA GTATTATCTA TATTCCAGAT ATAGATAATT GGTTGAATGT CTTACCTTTG 
ACAGCTATTA CGACCTTTTC TTCTATGCTT GAGAGACTTG ATTTTTCGGA TCAAATACTC 
TTTCTTGCCT TATCTTCAAG TCCGTTAAGT GAATTGCATC CTCAATTAAG AGAATGGTTC 
TCTTCAAAGC AATCCGTTTA CTCATTACAG TATCCGACTC GTGATAGCAT TATCGCATTT 
TTTCAGCCAA TTTTGGAACT AATTAAAGCC TCACCAACCG AACTTCCTGG TGGCATACCT 
CGAAAACGCA GAGTTTTACC AGAGTTACCT CTTGCTCCCG ATCCTCCACC ATTTACCAGT 
CAAAAAATTA CCCTAAAACA AACTAAGCAG GCCGACATGC GTCTTCTTAA CAAGCTAAAA 
ATAAAGCTGA ATGCCCTACT TGGAAGCCTT CGAGCAAGAT ATCGAAAGTT TAAGAAACCA 
TTGATTGATT TCAATGATAT ATACTGTGTA GATCCCGAAA CAGGTCATTC TTATCGTAGT 
CGCGAGGAAT GCCATTATGA GTTTGTTGAT GATGTAGTTA AGCAAATTGG AAGCGATCAA 
AAGTTTAGCA TGATGAGTTT AGAAGAGATT GAGAAGCGAA CTTGGGATAA TTGCTACTGT 
ACTCCGAAAC AGTTTGTACA TGATATTAAG CTCATTTTAC GTGATGCACT GCAACTGGAA 
GATTCAGAAA CTATCAAAAG AGCCCAGGAA ATGTATGCAA ACGTTCTCTT GGGTGTCGAA 
GATATGGAAG ACGATCAATT TTCCCAGAGA TGTGAAAGGA TGGCGTTACG TGAGGCTGAA 
CGAAGAAAAT TAAGGCACGG AAAGCTTCAA AAACATTTGG ATGAAACAAA AGCAGATATG 
CAGTTTACTT CTGAGAAGCC TTCTGTTGAC GAATCAATTA CTGAAGTTGA CGATGCCATA 
AAAGATGGAC CACCCGTACT AGCTGAAACC CTTACCAATT CACTGATGGA GGATGTTGGG 
CCGGAGAACG TTGACATGGA CATAGAAGAT AATGAAATTT TCACTAATCA GTCTACTATG 
TCTGTTCCTT CCATGTTAGT TGAGAATGAA GAGTCTCCCA AGCCTGACGA ATATATTGAT 
CAAAAAGACA AAGTACAATC TCCATTGTTA AATGGGAAGA GTCCAGTAGG CGTTCCATCA 
GAAGCCGCAT TACGTGTTTC TACTGATGTC TCTACGAATA TCTCTTCTAA TGGCCGTGCT 
GATATCCCTG TTGACACCCT CATCACGTCT CCTGCTGATG TTCCTAATAA TGCTCCTACT 
GATGCCCATA ATATTACATC CGCTGATGGT CATATTGAGA ATATCGAACA AGAGGTGGTA 
TTCCCTGATT TGGTGTTTGA TGAGGACAGA CTTACTCCAT TGAAGCAGTT GCTTATTGAT 
TCAACAACAG GCTTCACCGT TGACCAGCTT TTACATCTTC ATTCATTTTT GTACCAGATC 
ATTTGGAATA CGAAGTCCGA ATGGAATAGA AACTCTGTGG TTGATGAATG CGAGCGAGCA 
GTAAAAGAGT TTATGATTAA TGCACTACAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594020.1
CDS	1368..4940
Translation	MKEEASEHGGSADETQELSPVSDSSDEMPNNAKRRRRSQSMIANKRIHQAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSEGDDEPFEVSESSALEDELSDSEDSFIRSVRSKPKYKPGTRRSTRLRNRRSQDEEESEEEHRPILRERTSRINYSVPLAFPPVDEMDGDPSSQVNQSRSRKTHSELAITKLLRQQVSSFMPYIDSSGSESESDNTRIKKSSAKTIKALTDPANSGGPPDFGRIREKSDLADSDPLGVDSSLSFESVGGLDNYINQLKEMVMLPLLYPEIFQRFNMQPPRGVLFHGPPGTGKTLMARALAAACSSENKKVSFYMRKGADCLSKWVGEAERQLRLLFEEAKSTQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMESRGQVIIIGATNRPDAVDPALRRPGRFDREFYFPLPDRDARKKIIEIHTRNWDPPVPEWLCSMLAEKSKGYGGADLRALCTEAALNSIKRTYPQLYRSTKRLQIDPKTIKVKVKDFVMSMKRMIPSSERSSISPSKPLSPELKPLLNEAFQDIEKTLQKLMPVASKLNPLEEVMYDDPKENDFEYQQRLETFETLRIYKPRFLICGRKGLGQTALGPAILQQYEGVHVQSFDMSTLLQDSTQSIETSIIHLFLEVRRHTPSIIYIPDIDNWLNVLPLTAITTFSSMLERLDFSDQILFLALSSSPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPTELPGGIPRKRRVLPELPLAPDPPPFTSQKITLKQTKQADMRLLNKLKIKLNALLGSLRARYRKFKKPLIDFNDIYCVDPETGHSYRSREECHYEFVDDVVKQIGSDQKFSMMSLEEIEKRTWDNCYCTPKQFVHDIKLILRDALQLEDSETIKRAQEMYANVLLGVEDMEDDQFSQRCERMALREAERRKLRHGKLQKHLDETKADMQFTSEKPSVDESITEVDDAIKDGPPVLAETLTNSLMEDVGPENVDMDIEDNEIFTNQSTMSVPSMLVENEESPKPDEYIDQKDKVQSPLLNGKSPVGVPSEAALRVSTDVSTNISSNGRADIPVDTLITSPADVPNNAPTDAHNITSADGHIENIEQEVVFPDLVFDEDRLTPLKQLLIDSTTGFTVDQLLHLHSFLYQIIWNTKSEWNRNSVVDECERAVKEFMINALQ

Target ORF information:

RefSeq Version	NM_001019446.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- ATPase with bromodomain protein (predicted) (SPAC31G5.19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019446.2

ORF Insert Sequence:

ATGAAGGAAG AAGCAAGTGA ACATGGTGGA TCTGCGGATG AAACTCAAGA GTTGTCACCA 
GTGTCCGATT CTTCTGATGA GATGCCCAAC AATGCTAAGC GTCGTCGCCG TTCCCAGTCC 
ATGATTGCTA ACAAACGCAT TCATCAAGCT TTCCAGGAAG ATGAAGGTGA CGAGGATTGG 
GAAGAAGAGG AACATAAGCC CAAAGCGAAA CGGCGATATA ATACTCGGTC AAATGAAAGC 
TTTTCAGAGG GTGATGATGA ACCTTTTGAA GTATCAGAGT CCTCGGCTTT GGAAGATGAA 
CTATCAGATT CTGAAGATTC TTTTATACGA TCAGTCCGTT CTAAACCCAA GTACAAACCT 
GGAACTCGTC GCTCAACACG CCTTAGAAAT CGTAGATCAC AGGATGAAGA AGAATCCGAA 
GAAGAGCATA GACCTATACT GAGAGAACGG ACAAGTCGTA TAAATTACAG CGTTCCGTTG 
GCTTTCCCTC CAGTCGATGA AATGGATGGT GATCCTAGCT CTCAAGTCAA TCAAAGCAGG 
AGTAGGAAGA CGCATTCTGA ACTTGCTATT ACTAAGCTTC TCCGACAACA AGTTAGTAGT 
TTTATGCCAT ATATTGATTC TAGTGGGTCT GAATCTGAAA GCGACAATAC AAGAATTAAA 
AAGTCGTCTG CAAAAACCAT AAAAGCTTTG ACTGACCCAG CTAATTCCGG TGGGCCGCCT 
GATTTTGGCC GTATCAGGGA GAAAAGCGAT CTTGCCGACT CAGATCCTCT GGGAGTCGAC 
TCTTCTTTAT CCTTCGAATC CGTCGGTGGA CTGGACAATT ACATTAATCA ATTAAAAGAA 
ATGGTCATGC TTCCTTTGTT ATACCCTGAA ATATTTCAGC GTTTTAACAT GCAACCACCA 
AGGGGAGTAC TTTTTCATGG TCCTCCTGGT ACAGGAAAAA CTTTAATGGC TCGAGCTCTT 
GCTGCAGCTT GTTCTTCCGA AAACAAAAAA GTATCTTTCT ATATGAGAAA AGGCGCTGAT 
TGCCTCAGTA AATGGGTTGG CGAGGCTGAG CGTCAGCTTC GATTACTTTT CGAAGAAGCA 
AAAAGTACTC AACCAAGTAT AATTTTCTTT GATGAAATAG ATGGTCTGGC TCCTGTGAGA 
TCGAGTAAAC AAGAGCAAAT TCACGCATCT ATTGTTTCTA CATTATTGGC GTTAATGGAT 
GGCATGGAAA GCCGAGGCCA GGTCATTATA ATAGGTGCAA CAAACCGACC TGACGCAGTT 
GATCCAGCTC TCCGTCGTCC TGGTCGGTTT GACAGAGAAT TTTATTTTCC TTTGCCTGAT 
CGTGATGCCA GGAAAAAGAT AATAGAGATT CACACGCGAA ATTGGGATCC ACCAGTTCCT 
GAATGGCTAT GTTCGATGCT TGCCGAAAAG TCAAAAGGCT ATGGTGGTGC AGATTTGCGT 
GCTCTATGCA CGGAGGCTGC TCTCAACTCT ATCAAACGTA CCTATCCTCA ATTATATAGG 
AGTACAAAGC GTCTTCAAAT TGATCCTAAA ACCATAAAAG TGAAGGTAAA GGACTTTGTA 
ATGTCGATGA AACGCATGAT CCCTTCTTCT GAGCGCTCAT CAATATCTCC AAGTAAGCCT 
CTATCACCGG AATTGAAACC ACTACTCAAC GAAGCTTTTC AAGATATTGA GAAGACTTTA 
CAGAAATTAA TGCCTGTTGC CTCTAAATTA AATCCTCTTG AGGAAGTAAT GTATGACGAT 
CCAAAAGAAA ATGACTTTGA GTACCAACAG CGCTTGGAGA CTTTTGAAAC TCTAAGAATT 
TATAAGCCTA GATTCCTTAT TTGTGGTAGA AAGGGACTGG GACAAACCGC GCTTGGTCCA 
GCCATTTTGC AGCAATATGA AGGAGTGCAT GTTCAATCAT TTGACATGTC TACTCTACTT 
CAAGACTCCA CTCAAAGTAT CGAGACCTCT ATAATTCATC TCTTTTTAGA AGTAAGGCGT 
CACACTCCAA GTATTATCTA TATTCCAGAT ATAGATAATT GGTTGAATGT CTTACCTTTG 
ACAGCTATTA CGACCTTTTC TTCTATGCTT GAGAGACTTG ATTTTTCGGA TCAAATACTC 
TTTCTTGCCT TATCTTCAAG TCCGTTAAGT GAATTGCATC CTCAATTAAG AGAATGGTTC 
TCTTCAAAGC AATCCGTTTA CTCATTACAG TATCCGACTC GTGATAGCAT TATCGCATTT 
TTTCAGCCAA TTTTGGAACT AATTAAAGCC TCACCAACCG AACTTCCTGG TGGCATACCT 
CGAAAACGCA GAGTTTTACC AGAGTTACCT CTTGCTCCCG ATCCTCCACC ATTTACCAGT 
CAAAAAATTA CCCTAAAACA AACTAAGCAG GCCGACATGC GTCTTCTTAA CAAGCTAAAA 
ATAAAGCTGA ATGCCCTACT TGGAAGCCTT CGAGCAAGAT ATCGAAAGTT TAAGAAACCA 
TTGATTGATT TCAATGATAT ATACTGTGTA GATCCCGAAA CAGGTCATTC TTATCGTAGT 
CGCGAGGAAT GCCATTATGA GTTTGTTGAT GATGTAGTTA AGCAAATTGG AAGCGATCAA 
AAGTTTAGCA TGATGAGTTT AGAAGAGATT GAGAAGCGAA CTTGGGATAA TTGCTACTGT 
ACTCCGAAAC AGTTTGTACA TGATATTAAG CTCATTTTAC GTGATGCACT GCAACTGGAA 
GATTCAGAAA CTATCAAAAG AGCCCAGGAA ATGTATGCAA ACGTTCTCTT GGGTGTCGAA 
GATATGGAAG ACGATCAATT TTCCCAGAGA TGTGAAAGGA TGGCGTTACG TGAGGCTGAA 
CGAAGAAAAT TAAGGCACGG AAAGCTTCAA AAACATTTGG ATGAAACAAA AGCAGATATG 
CAGTTTACTT CTGAGAAGCC TTCTGTTGAC GAATCAATTA CTGAAGTTGA CGATGCCATA 
AAAGATGGAC CACCCGTACT AGCTGAAACC CTTACCAATT CACTGATGGA GGATGTTGGG 
CCGGAGAACG TTGACATGGA CATAGAAGAT AATGAAATTT TCACTAATCA GTCTACTATG 
TCTGTTCCTT CCATGTTAGT TGAGAATGAA GAGTCTCCCA AGCCTGACGA ATATATTGAT 
CAAAAAGACA AAGTACAATC TCCATTGTTA AATGGGAAGA GTCCAGTAGG CGTTCCATCA 
GAAGCCGCAT TACGTGTTTC TACTGATGTC TCTACGAATA TCTCTTCTAA TGGCCGTGCT 
GATATCCCTG TTGACACCCT CATCACGTCT CCTGCTGATG TTCCTAATAA TGCTCCTACT 
GATGCCCATA ATATTACATC CGCTGATGGT CATATTGAGA ATATCGAACA AGAGGTGGTA 
TTCCCTGATT TGGTGTTTGA TGAGGACAGA CTTACTCCAT TGAAGCAGTT GCTTATTGAT 
TCAACAACAG GCTTCACCGT TGACCAGCTT TTACATCTTC ATTCATTTTT GTACCAGATC 
ATTTGGAATA CGAAGTCCGA ATGGAATAGA AACTCTGTGG TTGATGAATG CGAGCGAGCA 
GTAAAAGAGT TTATGATTAA TGCACTACAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC31G5.19 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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