NLRC5 gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_023494216.1	XP_023349984.1	protein NLRC5 isoform X1
XM_012553827.3	XP_012409281.1	protein NLRC5
XM_023494218.1	XP_023349986.1	protein NLRC5 isoform X1
XM_023494215.2	XP_023349983.1	protein NLRC5
XM_023494219.1	XP_023349987.1	protein NLRC5 isoform X2
XM_012553827.1	XP_012409281.1	protein NLRC5
XM_023494217.1	XP_023349985.1	protein NLRC5 isoform X1
XM_023494215.1	XP_023349983.1	protein NLRC5 isoform X1
XM_012553827.2	XP_012409281.1	protein NLRC5 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa118850	XM_023494217.1 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSa118850	XM_023494218.1 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSa118850	XM_023494216.1 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSa118850	XM_012553827.3 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa118850	XM_023494215.2 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150289	XM_023494219.1 Latest version!	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSa118850	XM_012553827.1	Sarcophilus harrisii NLR family, CARD domain containing 5 (NLRC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSa118850	XM_023494215.1	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSa118850	XM_012553827.2	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

NLRC5 ( XM_023494216.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349984.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_023494216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349984.1
CDS	357..6038
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_023494216.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494216.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_012553827.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409281.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

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CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_012553827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012553827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409281.1
CDS	605..6286
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_012553827.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553827.3

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_023494218.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349986.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_023494218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349986.1
CDS	507..6188
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_023494218.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494218.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_023494215.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349983.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_023494215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023494215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349983.1
CDS	666..6347
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_023494215.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494215.2

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_012553827.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409281.1 Sarcophilus harrisii NLR family, CARD domain containing 5 (NLRC5), mRNA.

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CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_012553827.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409281.1
CDS	274..5955
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_012553827.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family, CARD domain containing 5 (NLRC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553827.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_023494217.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349985.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.

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CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_023494217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349985.1
CDS	197..5878
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_023494217.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494217.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_023494215.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349983.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

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CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_023494215.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349983.1
CDS	516..6197
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_023494215.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494215.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_012553827.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409281.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

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CloneID	OSa118850
Clone ID Related Accession (Same CDS sequence)	XM_023494216.1 , XM_012553827.3 , XM_023494218.1 , XM_023494215.2 , XM_012553827.1 , XM_023494217.1 , XM_023494215.1 , XM_012553827.2
Accession Version	XM_012553827.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012553827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409281.1
CDS	455..6136
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEILLAWNSLGNEGATELAYILPQMKALKKFDLEGNQIGSLGAQLLAKGLAKNPGIQVIRLWKNPVPKDTAQLLQGQDPRLNFS

Target ORF information:

RefSeq Version	XM_012553827.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553827.2

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT TGGCCTGGAA TTCCCTTGGG 
AATGAAGGGG CCACTGAGCT AGCCTATATT CTACCTCAGA TGAAAGCACT GAAGAAATTT 
GATTTGGAAG GCAATCAGAT TGGCTCCCTG GGAGCACAGC TGCTGGCAAA GGGGCTAGCC 
AAGAATCCTG GAATTCAAGT CATACGCTTG TGGAAGAATC CAGTCCCCAA GGACACAGCC 
CAGTTGCTCC AGGGCCAGGA TCCCAGACTG AACTTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_023494219.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023349987.1 Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X6, mRNA.

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CloneID	OSa150289
Clone ID Related Accession (Same CDS sequence)	XM_023494219.1
Accession Version	XM_023494219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein NLRC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816660.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023349987.1
CDS	517..5958
Translation	MDSLSWQNDEESLQQHLVGFLSKDLEWLILKVKFFLPDLDLTPVDDLPDPTQKVSLLLDKLRAHSLSTWRTFIHCVCMEHTMPLHLETMLLSVWGMHSEIPSQNIFCKDALPKSQLLHSGVKRPSSEIGSSPPRKWSRQQQLELVRKYEKRLRTLFLKKYEDVPGRTSGQWQQWPFCFSQTYVDPVVRPGKVAWLKEGPEKSQVETLAEPLEVEDGANFKVQDLFRGQSGSGTKVTVLLGKPGTGKSTLVQRICRKWARGELDRFSQVFLFEFRQLNLVKQELTLPQLLFEVYENLEEEPGAVFQHLQENDHQVLLIFDGLDECLFPWPGRGPCARDSQDPFLSRPVASLFCSLCSGSLLPGCWVVTTSRPGKIPDFLLETATSVFEVWGFDQLRIENYVNNFFQGLISPERALACLRASAQLQSLCSNPALCSIACFCLHHLLCQDHTIKFPTTVTQLFTQMLHIFVGQKQGTPGDKGTWLSKDSQALLGLGELAFKGLNEKQIVFYPGHIPEQVVAFAATYNLLSSFSVRTGTGQRDTCYAFVHFSLQEFFAALYLMVSPSIDSSKLRHHFYLKSRWPLRTEAKTELLDRFHTFLSGFSSQTYLSFLTRLSQQGRAWVSTKQAAVRQVLRGLATQSLTGPKMIELCHCVGETQDPELMGQAGRSLPFSLDFHNFPLNHSDLTALTSLINHAPASIYLNFSGCSLESQCLNALTGCGKIESFRVRSRKCRDAFAEALSKILPTMGNLKKLELTGNNISVQGIDHLAQAFTDCLQLEEINLQDNRLNDSSCLKIITILHKMQRLQKIDLSQNAVSVQTVLALAKEAVSYPRVTTLEIRQAQMILLFFVSSGKALEMQRSQNVKKNEPSDKIVTQDRKLTFRLQKCNLAVHHAKEIAEMLKHRPYLEEVDLSGNHLEDEGCRELAEALPDLQIAKQFNISDNGLSESGVYYLLTSANACKNVMELDISLKNKTAVFKFAGDQEKDQAPLGAAVSNPQLSSNSSEPVSSLSARVIRLRHCGFQCRELEKLCLKLGNCSHLDLSNNRLGNKGIEKLIQLLPSLRSLQFLTLNDNRVSLKAVFCLASTFSDLEWVHEVDISLGRQQSIHVRFLERTRKRSVSEEWTKQKTQQESLEWGAQFPKSFCLRECQLEPPVLEKLIQILKTCQGLLEIKFSHVTMNDQSIGMLLNHLPQFHNLNLVQFSQTTLSLNSVFSLASAISLCEQIQKMEVRSLKYACLYFTPGKRKGVSCGLTDCCINQMDMKQLCCILGQCKDLSHLNLSGNLLGDEGLKYLLEHLPQMNLSGSLNLSHNGISEEGALLFTVALGTCRQAHEVSVSLNLEQKLLIHFTEHQEPRKTLRLTGFNLQQEQIKKLATLVGQVPKLNDLILATNTMSLAGLQLLLNSVRMPSGILRISVEEPWVTRTSVTTLLEASVQASGNITKISICRQQSLLQIEETFPQKTGNFAADTLRPIQCDQGASLTFPYDLSQCDQGDNQTLVLRQMVEKYMRLQELSWSQVYLCDTSSQLLKTLFPSLLELKKFSLTASTVSPEGVCHVASGLSHCNTMEELDLSKNEFSVEGTRVLIGALEGKCQLRSLNLSFLQLDNLALSELAQRLQGMSLLQKLDLSHSGLSSESCFSLSDSLKDATNLKDLNLSHNKIEDAGVQHLSAILPKLLQLSKLDLSWNSISPAGGRTLAEALACCEQVEELHLGANTMGDLMAIRLAQNLPPHLKILYLCSNKIGAKGTLSLGQALVRCQWIEEISLAENIFAEWILPLSKGLLKLRKINLTSCRINDQMIKPLASSLVLCHDLEEIL

Target ORF information:

RefSeq Version	XM_023494219.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii NLR family CARD domain containing 5 (NLRC5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494219.1

ORF Insert Sequence:

ATGGACTCCC TAAGCTGGCA GAATGATGAG GAAAGCCTTC AACAGCATCT GGTTGGATTT 
CTCAGTAAGG ATCTTGAATG GCTGATCTTA AAGGTCAAGT TCTTCCTGCC TGACCTAGAC 
CTGACTCCTG TGGATGACCT CCCAGATCCC ACCCAGAAAG TCTCCCTGCT CCTTGACAAA 
CTGAGAGCCC ACAGCCTCTC CACATGGCGG ACCTTCATCC ATTGTGTCTG CATGGAACAC 
ACCATGCCCC TACACCTGGA GACCATGCTC CTGAGTGTCT GGGGTATGCA TAGTGAGATT 
CCAAGCCAGA ACATTTTCTG TAAAGATGCC CTTCCTAAGT CCCAGCTCCT TCATTCAGGC 
GTCAAGAGAC CTTCCTCTGA GATAGGATCC TCACCTCCTC GAAAATGGTC CAGGCAGCAG 
CAGCTGGAGT TGGTCAGAAA GTATGAGAAG CGCCTGCGGA CGTTGTTCCT GAAGAAGTAT 
GAGGATGTAC CAGGAAGGAC GTCGGGGCAG TGGCAACAAT GGCCGTTCTG CTTCAGCCAG 
ACCTATGTGG ACCCCGTCGT GCGGCCGGGC AAGGTAGCCT GGCTGAAGGA GGGCCCAGAG 
AAGTCGCAGG TTGAGACTCT GGCAGAGCCT CTTGAGGTGG AGGATGGGGC TAATTTCAAA 
GTCCAGGACT TGTTCAGAGG CCAGTCGGGT TCAGGCACCA AGGTGACAGT CCTGCTGGGG 
AAGCCAGGGA CAGGGAAAAG CACGCTGGTG CAGCGTATCT GCCGGAAGTG GGCGCGTGGG 
GAGCTGGACC GCTTCAGCCA GGTGTTCCTT TTTGAGTTCC GCCAGCTCAA CTTGGTGAAG 
CAGGAGCTGA CCCTTCCCCA GCTCCTCTTT GAGGTATATG AGAACCTGGA GGAGGAGCCC 
GGCGCCGTCT TTCAGCATCT GCAGGAGAAC GACCATCAGG TTCTGCTGAT CTTTGATGGG 
CTGGATGAGT GCCTCTTCCC CTGGCCGGGC CGAGGGCCCT GTGCCCGGGA TTCCCAAGAC 
CCTTTCCTGT CGCGGCCTGT TGCCAGCCTT TTCTGCAGCC TCTGTAGTGG ATCCCTCCTG 
CCAGGCTGCT GGGTGGTAAC CACCTCACGC CCCGGAAAGA TCCCAGACTT CCTGCTGGAA 
ACAGCCACAT CCGTGTTTGA GGTCTGGGGG TTTGACCAGC TGCGGATTGA GAATTACGTC 
AACAACTTTT TCCAGGGACT GATCTCCCCA GAGAGGGCCC TGGCCTGCCT CAGGGCCAGT 
GCCCAGCTCC AGAGCCTGTG CTCCAACCCA GCTCTGTGCT CTATCGCCTG CTTCTGCCTC 
CACCACCTCC TATGCCAGGA CCACACAATT AAGTTCCCCA CAACAGTCAC CCAGCTCTTT 
ACTCAGATGC TCCATATCTT TGTAGGTCAG AAACAGGGGA CCCCTGGAGA CAAAGGCACC 
TGGCTGAGCA AGGACTCCCA GGCCCTGCTG GGCCTTGGAG AACTAGCCTT CAAGGGGCTG 
AATGAAAAGC AGATAGTCTT CTATCCAGGT CACATCCCTG AGCAGGTGGT GGCCTTTGCA 
GCCACCTACA ACCTCCTGTC CTCCTTCTCA GTCAGGACAG GGACTGGGCA GCGGGACACA 
TGCTATGCCT TTGTCCACTT TAGCCTACAA GAATTCTTTG CAGCCCTGTA CCTGATGGTG 
AGCCCTTCCA TAGACAGCAG CAAGCTGCGC CACCACTTCT ACCTCAAGTC CAGATGGCCC 
CTGAGGACTG AAGCTAAGAC GGAGCTCCTG GATCGCTTCC ACACCTTCTT GTCGGGCTTC 
TCCTCTCAGA CCTATCTGTC CTTCCTGACC CGGTTGTCCC AGCAGGGCAG AGCCTGGGTC 
AGTACTAAGC AAGCGGCCGT GCGGCAAGTG TTACGGGGCC TGGCCACCCA GAGCCTGACA 
GGCCCCAAGA TGATAGAGCT GTGCCACTGT GTGGGCGAGA CTCAGGACCC AGAGCTGATG 
GGTCAGGCTG GCAGGAGCCT GCCTTTCAGT CTGGACTTCC ATAATTTCCC CCTGAACCAC 
AGCGATCTGA CCGCCCTGAC TTCTCTCATC AACCATGCTC CTGCTTCTAT ATATCTCAAT 
TTTTCTGGCT GCTCTTTAGA GTCCCAGTGC CTCAATGCCC TAACTGGCTG TGGGAAAATT 
GAGAGTTTCA GAGTTAGAAG TAGGAAGTGC AGAGATGCTT TTGCAGAAGC CCTCTCTAAG 
ATATTGCCAA CAATGGGAAA TCTGAAGAAA CTTGAATTGA CTGGAAATAA CATCTCAGTT 
CAAGGCATTG ACCATCTGGC ACAAGCATTT ACAGACTGTC TTCAGCTGGA GGAGATTAAT 
TTGCAAGACA ACAGATTGAA TGATTCTTCA TGTCTAAAGA TCATCACGAT CCTTCATAAG 
ATGCAACGGT TACAGAAAAT TGACTTAAGC CAAAATGCAG TTTCTGTCCA GACAGTCCTG 
GCCCTGGCAA AGGAAGCAGT TTCTTATCCC CGAGTCACCA CCTTGGAGAT TAGGCAAGCA 
CAAATGATCC TTCTCTTCTT TGTATCTTCG GGAAAGGCTC TGGAAATGCA AAGATCCCAG 
AATGTGAAGA AAAATGAGCC TTCAGATAAA ATTGTGACTC AAGACAGAAA GCTAACATTC 
AGGCTTCAGA AATGCAACCT TGCTGTCCAC CATGCAAAGG AGATAGCTGA GATGCTCAAA 
CACAGGCCCT ACTTGGAAGA AGTGGATTTA TCAGGAAACC ATTTGGAAGA TGAAGGCTGC 
CGGGAGTTAG CAGAGGCCCT ACCTGATTTG CAGATTGCCA AACAGTTTAA CATCAGTGAT 
AATGGGCTGT CTGAGTCCGG GGTGTATTAC TTGCTGACTT CAGCCAATGC CTGCAAGAAT 
GTGATGGAGC TAGATATCAG CCTGAAGAAC AAAACTGCTG TCTTCAAATT TGCTGGGGAC 
CAGGAGAAGG ATCAAGCCCC ACTCGGAGCT GCAGTTTCAA ATCCCCAGCT ATCCAGCAAC 
AGCTCTGAGC CAGTTTCATC CCTTTCTGCC AGGGTGATCA GGTTAAGACA CTGTGGTTTC 
CAGTGCAGGG AACTTGAGAA GCTGTGTCTA AAGCTGGGCA ACTGCAGCCA TCTTGACTTA 
TCCAACAACC GGTTGGGCAA CAAAGGCATT GAGAAATTGA TTCAGCTCCT TCCAAGCCTG 
AGATCCTTGC AATTCCTGAC CTTGAATGAT AATAGGGTAT CCTTGAAAGC TGTGTTCTGC 
TTAGCTTCAA CCTTCTCTGA CCTGGAGTGG GTCCATGAAG TAGATATCAG TTTGGGAAGA 
CAACAATCTA TCCATGTGAG ATTCCTTGAG AGAACAAGGA AACGAAGTGT CTCTGAAGAG 
TGGACAAAGC AGAAGACTCA GCAGGAATCT CTAGAATGGG GTGCACAGTT TCCCAAGAGC 
TTTTGTCTCA GAGAGTGTCA ATTGGAACCA CCAGTCTTAG AGAAGCTGAT CCAGATTCTG 
AAGACATGCC AGGGACTACT AGAGATCAAA TTCTCCCATG TTACCATGAA TGATCAGAGC 
ATTGGGATGC TACTGAACCA CTTACCCCAG TTCCACAATC TGAACCTGGT GCAATTCAGC 
CAAACCACTT TGTCTCTAAA TAGTGTTTTC TCGCTGGCCA GTGCCATTAG CCTATGTGAA 
CAAATTCAAA AGATGGAAGT CAGGTCCCTG AAGTATGCCT GTCTGTACTT TACTCCTGGC 
AAGAGGAAAG GAGTGTCCTG TGGGCTCACA GATTGTTGCA TCAACCAAAT GGATATGAAG 
CAACTGTGTT GTATACTAGG GCAGTGCAAA GATCTGAGTC ACCTTAATTT GTCAGGAAAC 
TTGCTGGGTG ATGAAGGACT CAAATATCTT CTGGAGCATT TACCTCAGAT GAATCTCTCT 
GGCTCACTTA ACCTAAGCCA CAATGGCATC TCTGAGGAGG GTGCTCTGCT CTTCACTGTT 
GCTCTCGGCA CCTGCAGACA GGCCCATGAG GTCTCAGTAA GCCTGAATCT AGAGCAGAAA 
CTTCTGATAC ACTTCACAGA ACACCAAGAG CCCAGAAAAA CTCTGAGGCT AACCGGATTC 
AACCTCCAGC AGGAGCAAAT CAAAAAGTTG GCCACCCTGG TGGGACAAGT GCCAAAGCTG 
AATGATCTCA TATTGGCCAC TAACACAATG TCACTAGCAG GGTTGCAACT TCTCCTGAAT 
TCAGTGAGGA TGCCATCAGG GATCCTGAGG ATCAGTGTTG AAGAGCCATG GGTGACCAGG 
ACCAGTGTTA CCACCTTGCT GGAGGCAAGT GTTCAGGCTT CAGGCAACAT CACCAAGATA 
AGCATATGCA GACAACAATC TCTCCTCCAG ATTGAAGAGA CATTTCCCCA GAAGACGGGG 
AATTTTGCAG CTGACACTTT AAGACCGATC CAGTGTGACC AAGGAGCCAG CCTGACTTTT 
CCATATGATC TGTCCCAGTG TGACCAAGGA GACAACCAGA CCCTTGTCCT CAGGCAGATG 
GTTGAAAAGT ACATGAGACT ACAGGAGCTT AGCTGGTCCC AGGTTTACTT GTGTGATACC 
AGCAGCCAGC TACTCAAGAC TCTGTTTCCG TCTCTTCTTG AGTTGAAGAA ATTTAGCCTG 
ACAGCCAGCA CAGTGAGCCC TGAGGGGGTC TGCCATGTAG CGTCTGGACT AAGTCACTGC 
AATACTATGG AAGAGCTAGA TCTATCCAAG AATGAGTTCA GTGTGGAAGG TACTAGGGTA 
CTAATTGGAG CTCTGGAAGG GAAGTGCCAA CTTAGGAGTC TGAACCTCAG TTTCCTACAG 
CTGGATAACC TAGCATTGAG TGAACTAGCC CAAAGACTTC AAGGAATGTC ACTTCTGCAG 
AAACTTGACT TGAGCCACAG TGGCCTCAGC AGTGAAAGCT GTTTCTCCCT GTCAGATTCC 
CTCAAGGATG CTACCAACTT AAAAGATCTG AACTTGAGCC ACAACAAGAT CGAAGATGCT 
GGGGTGCAGC ATTTATCAGC CATCCTGCCT AAGCTGCTAC AGCTCAGTAA ACTTGACCTT 
TCATGGAACA GCATCAGCCC AGCAGGAGGC AGGACACTGG CTGAAGCACT GGCATGCTGT 
GAGCAGGTGG AGGAGTTACA CTTAGGGGCC AATACAATGG GGGACCTGAT GGCCATAAGG 
CTGGCCCAGA ACCTGCCACC TCACCTGAAA ATCTTGTACC TTTGTTCCAA TAAGATTGGT 
GCTAAGGGGA CACTGAGCCT GGGTCAGGCC CTTGTCAGAT GCCAATGGAT AGAAGAGATC 
AGCTTGGCAG AGAACATTTT TGCTGAATGG ATCCTGCCTC TCTCAAAGGG ACTCCTGAAA 
CTCAGAAAGA TAAACCTTAC ATCCTGCAGG ATTAATGACC AGATGATCAA ACCTCTTGCC 
AGCAGCTTGG TCCTCTGTCA TGATCTTGAG GAGATCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NLRC5
Entrez Gene ID	100920685
Full Name	NLR family CARD domain containing 5
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NLRC5 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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