SEC3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC3
Entrez Gene ID	856726
Full Name	GTP-Rho binding exocyst subunit SEC3
Synonyms	PSL1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178899.1	NP_010924.1	GTP-Rho binding exocyst subunit SEC3

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D13530g	XP_453666.1
Saccharomyces cerevisiae (baker's yeast)	SEC3	NP_010924.1
Eremothecium gossypii	AGOS_ADR012C	NP_984108.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate structural details of the interaction of exocyst complex Sec3p and Exo70p with lipid bilayers containing phosphatidylinositol (4,5)-bisphosphate (PIP2) by molecular dynamics simulations.
Title: Membrane targeting of the yeast exocyst complex.

only the ectopically located Sec3p subunit is capable of recruiting secretory vesicles to mitochondria.
Title: The role of Sec3p in secretory vesicle targeting and exocyst complex assembly.

Residues Phe77, Ile115 and Leu131 of Sec3 bind to an extended hydrophobic surface formed around switch regions I and II of Rho1.
Title: Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.

The interactions of Sec3 with phospholipids and Cdc42 play important roles in exocytosis and polarized cell growth.
Title: Membrane association and functional regulation of Sec3 by phospholipids and Cdc42.

Assembly of the exocyst occurs when the first subset of subunits, delivered on vesicles, joins Sec3p and Exo70p on the plasma membrane. Exocyst assembly serves to both target and tether vesicles to sites of exocytosis.
Title: Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p.

The following SEC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05495	NM_001178899.1 Latest version!	Saccharomyces cerevisiae S288C GTP-Rho binding exocyst subunit SEC3 (SEC3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC3 ( NM_001178899.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010924.1 Saccharomyces cerevisiae S288C GTP-Rho binding exocyst subunit SEC3 (SEC3), partial mRNA.

Price & Availability↑

CloneID	OSi05495
Clone ID Related Accession (Same CDS sequence)	NM_001178899.1
Accession Version	NM_001178899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	GTP-Rho binding exocyst subunit SEC3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGGTCCT CGAAGTCTCC TTTTAAAAGG AAGTCTCACA GTCGTGAGAC ATCACACGAT 
GAAAACACAT CGTTTTTCCA CAAGAGAACA ATATCCGGTA GCAGTGCTCA CCATTCTAGA 
AACGTCAGTC AAGGTGCAGT ACCCTCTTCC GCACCACCTG TTTCTGGTGG AAATTATTCG 
CATAAGAGAA ACGTGTCGAG AGCTTCAAAT TCCTCTCAAA CTTCGAATTT TTTAGCCGAA 
CAATATGAAA GGGATAGGAA AGCCATAATT AATTGCTGCT TTTCCAGACC CGACCATAAG 
ACAGGTGAAC CGCCGAATAA TTATATTACA CATGTCCGGA TTATAGAAGA TTCAAAATTC 
CCTAGTTCAA GACCTCCTCC AGATTCAAAA TTGGAGAACA AAAAGAAAAG ACTCCTTATT 
TTGAGTGCCA AGCCCAACAA TGCCAAGCTA ATACAAATTC ATAAAGCAAG AGAAAACTCA 
GACGGCTCTT TTCAAATTGG TAGAACTTGG CAGTTGACCG AACTAGTAAG AGTAGAGAAG 
GACCTGGAGA TATCTGAAGG CTTCATTCTT ACCATGAGTA AAAAATACTA CTGGGAAACG 
AATTCTGCGA AGGAAAGAAC TGTTTTCATT AAATCTCTAA TTACTCTATA CATTCAAACA 
TTCGAAGGTC ATGTCCCTGA ATTGGTCAAT TGGGATTTAT CCCTTTTTTA TCTTGACGAG 
AGGAGTTATC AAAGAGCAGT CATCACAAAT CGCCCCGGTT CCGTATCTCC AATAAAGTCA 
CCTACAAGTA ATTTCACGAC AAACACTACC CAATCGGTGG GCTCTGTCCC CTTTTCAGCA 
CCTACTGAGA GGACAAGAAG GTCTGAAACA GAAAGCGTTA ATCCAGTGTC AACGCCTGCA 
AGCGTGGAAT ATCATGCAGG AATGAAATCT CTAAATAAGG CCCCCTATTC TTCCAATTCG 
ACATTGAATG AAGTGAATAA AAGATACGAG TTAGAGCAAC AACAGCAACA AGAAGAGGCT 
GAACTGAGAC GATTAGAAGA GCAAAAAAGA CTTCAGTTAC AAAAGGAAAA TGAGATGAAA 
AGATTAGAAG AGGAAAGAAG AATAAAGCAG GAGGAGAGGA AAAGGCAAAT GGAATTGGAA 
CACCAACGAC AACTCGAAGA AGAGGAACGA AAACGTCAAA TGGAACTGGA GGCTAAAAAG 
CAAATGGAGT TGAAACGTCA ACGGCAATTC GAAGAAGAGC AACGCCTAAA AAAAGAGAGA 
GAACTATTGG AAATACAGAG AAAACAAAGA GAACAAGAAA CAGCTGAGAG ATTAAAAAAA 
GAAGAGCAAG AAGCGCTTGC TAAAAAGGAG GAGGAAGAAA AAAGTAAAAG AAACAAAGTA 
GACAATGAAA GTTATACTCA AGAAATAAAT GGTAAGGTTG ATAACCTTTT GGAGGACTTA 
AACGCAGTTT TGGCGGAAGA AACAGAAACC ACCCCTACTA TGCAAAATGG TACTTACGTG 
CCCGAGAGAA GTACAGCTAG AGCTCATGAC CAATTAAAAA AACCACTAAA TATTGCCAAA 
GTTGAGTCAC TCGGTGGAAG CGATTTGAAT GACTCGATCA GTTTGAGTGA CGAGATAGCA 
GGTTTGAATA CATCTAACTT ATCAGGAGAG GACCAAGATG AGAAGAACGA TCTTTCGTTC 
GAGAAGGGAG ATGAGGTTAG GTATAGCAAT AATTTTGAAG GTGAAGCTCC TCACGTGTAC 
CATGAAGTGT CAATTATTCA AGAAGAAGCA CCTGCCGTTT CGCAGAAATT AATCCTTCCG 
GAAGAGAATA ACGAATCTGA AGCGCTGATT GAATCTAAAG AGGAGATAAA AACGATGGAA 
AATATTGACG ATGAGGTTTT ATTAGAAATA TTAACAGATA TCAATTGGTC CATTGAAGAC 
GATGCTGATA GTATGATTGA AAGGATTGAC TTAAGATTGG CGGAAACGGA ATACTTATTC 
AACCAAAATT TATTATCTTT GCAAAAAATT GGGCCTAATA TCCGCCCCTA CGAAGACAAG 
GTCAATGACG AATGTCATCG CATAATCCCC ACTCTCTCTT TATTCTTGAT GGAAATGAGC 
AATTTTTCTA ATGATATCGA AAACGTAGAG AGCCAAGATA ACGGATTACA AGTTGAAAGT 
GCTAACAAGA AACTTCTATG GAACACACTT GATGAACTTT TGAAAACTGT ATCTCTTGAT 
GAAATTTCCC TAAATCAGTT ATTAGAATGT CCAATAAGAG AGAAGAACTT GCCATGGATG 
GAAAACCAAT TGAATTTATT ATTAAAAGCA TTTCAAGCAA TAGGCAGCGA TGGAAATGAA 
GTAGAATATA ATCTAAGAGA AATTTCCGGA CTAAAGCAGA GGCTACAGTT TTATGAGAAA 
GTTACCAAGA TATTTCTTAA CAGAATTGTG GAGGAAATGC AGAAGAAATT CTCAAATATA 
CGTGGACAAG ATATCTCACA CGATCAAATG ATTAGAATTT TGACGACTTT ATTGATATTT 
TCTCCTTTAA TACTGTTCTG TAAAGAAATT TCACAAAAAT CGTATCAAGC AATTGTGGAA 
AATTGGAACG TCAGTATTCA ACCAGTATAC ATGGAGTTAT GGACTAAGAA AATATCACAA 
TTACAAGGCA TTGACACAAA TGATGAAAAA ATGAATGAGC TGAGCTTAAG CCAGTTACTA 
AATGAATGGG ACACATTTAG AAAAGAGAGA AAAACAAATG ATATCAACCC GGTTTTCAAG 
AATAGTTTTT CTCTTCTTAC CGAGTGCCTT CAAACCATGA GGCAAGAATG TATTGTTTAT 
CAAAATTTTG TCGAAGTGTT CTTTCATATA TCTTCAAAGC ATAATTTCGA AGAATACATT 
AAACATTTCA ATGATCCAGA TGCTCCTCCA ATATTATTAG ATACTGTGAA AGTAATGCAA 
TCTGATAGAG AAGCAGCCGT TATTGAAACC CAGTTGGTCT CAAGAATATT CCAACCTATT 
GTCACTAGGC TGTCCTCATA TTTTGTAGAA TTAGTGAAAG CCGAACCAAC GGTAGCCCCA 
GCCTTAACTT TTTACTTGGA GAATGAAATT AAAAGCTTAG AATCATCCAA CCATGAATTT 
TTATTGTCTG CAGTGACTAG AATGTACACA CAAATAAAAC AAGTGTGGTC TGATAATGTA 
GAAGAGCAGG TTTTGCACTT CGAAAGAATT TCTAACGCAA CCACCAATGG AGAAATTCTT 
CCAGGTATAC TTGATTTACC AGTAGGCTTG AAGAATTCAG AAGATTTGTT CCAATTTGCT 
AAGAGGTCTA TGGATATTAA AGATACCGAT GAAGGCTACG AGTCCATCGA ATTAATGAAT 
TCGAGCTTCA GGAAGCTAAG CATAGCTGCC ACACGATCGA TAACTCACAA AGAAGTTAAT 
TCAAGCATTA ATCCAAACCT TTCAGATACT GCTGCTTTAA ATAATGACTA TATGGAAACT 
ATTTCTCTCC TAGTGAACAG TAACTGGCTA ACTGAAATGC TTTCCATGTT AAACTTCAAT 
AAGGATGGTA TATTTGATAC GTCATTGCAA AACGTTAAGA AAGTTTTCGA TGTGGAAAAA 
GAATCTTATG CTTCCTTTCT ACTCCGCGAT ACTATGCCTA AACTAACAGC CTTTGTTTAT 
GGTGTGAGCA ACATCATTGA AAATACCAAC AATGTGAACA TGACCAATCC TTCAAGATGG 
GCCGCATATA GCAGGCAGAA CCTAGAGAAC ATTCTTTTAG CATACACTTC CCATGAAATT 
GAAACTCTAG TAAAAAGGCT GCACACTCAT ATGGTAAATG ACTTCGGTTA CCACCAAGAA 
AATGCCATAA ACAACGTCCT ATGCGATAAG TTGTGGTCGT GCATTCAAGG TCAAACCGTC 
TCACTATACT TGAAACTATA CACTGTAATT GATAAACACT ATAGAGGGAC AAACATACGC 
TTCACGAAGA ACGATATCAT AAGTGCGTTC GAGGAATACA AGAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010924.1
CDS	1..4011
Translation	MRSSKSPFKRKSHSRETSHDENTSFFHKRTISGSSAHHSRNVSQGAVPSSAPPVSGGNYSHKRNVSRASNSSQTSNFLAEQYERDRKAIINCCFSRPDHKTGEPPNNYITHVRIIEDSKFPSSRPPPDSKLENKKKRLLILSAKPNNAKLIQIHKARENSDGSFQIGRTWQLTELVRVEKDLEISEGFILTMSKKYYWETNSAKERTVFIKSLITLYIQTFEGHVPELVNWDLSLFYLDERSYQRAVITNRPGSVSPIKSPTSNFTTNTTQSVGSVPFSAPTERTRRSETESVNPVSTPASVEYHAGMKSLNKAPYSSNSTLNEVNKRYELEQQQQQEEAELRRLEEQKRLQLQKENEMKRLEEERRIKQEERKRQMELEHQRQLEEEERKRQMELEAKKQMELKRQRQFEEEQRLKKERELLEIQRKQREQETAERLKKEEQEALAKKEEEEKSKRNKVDNESYTQEINGKVDNLLEDLNAVLAEETETTPTMQNGTYVPERSTARAHDQLKKPLNIAKVESLGGSDLNDSISLSDEIAGLNTSNLSGEDQDEKNDLSFEKGDEVRYSNNFEGEAPHVYHEVSIIQEEAPAVSQKLILPEENNESEALIESKEEIKTMENIDDEVLLEILTDINWSIEDDADSMIERIDLRLAETEYLFNQNLLSLQKIGPNIRPYEDKVNDECHRIIPTLSLFLMEMSNFSNDIENVESQDNGLQVESANKKLLWNTLDELLKTVSLDEISLNQLLECPIREKNLPWMENQLNLLLKAFQAIGSDGNEVEYNLREISGLKQRLQFYEKVTKIFLNRIVEEMQKKFSNIRGQDISHDQMIRILTTLLIFSPLILFCKEISQKSYQAIVENWNVSIQPVYMELWTKKISQLQGIDTNDEKMNELSLSQLLNEWDTFRKERKTNDINPVFKNSFSLLTECLQTMRQECIVYQNFVEVFFHISSKHNFEEYIKHFNDPDAPPILLDTVKVMQSDREAAVIETQLVSRIFQPIVTRLSSYFVELVKAEPTVAPALTFYLENEIKSLESSNHEFLLSAVTRMYTQIKQVWSDNVEEQVLHFERISNATTNGEILPGILDLPVGLKNSEDLFQFAKRSMDIKDTDEGYESIELMNSSFRKLSIAATRSITHKEVNSSINPNLSDTAALNNDYMETISLLVNSNWLTEMLSMLNFNKDGIFDTSLQNVKKVFDVEKESYASFLLRDTMPKLTAFVYGVSNIIENTNNVNMTNPSRWAAYSRQNLENILLAYTSHEIETLVKRLHTHMVNDFGYHQENAINNVLCDKLWSCIQGQTVSLYLKLYTVIDKHYRGTNIRFTKNDIISAFEEYKNA

Target ORF information:

RefSeq Version	NM_001178899.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C GTP-Rho binding exocyst subunit SEC3 (SEC3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178899.1

ORF Insert Sequence:

ATGAGGTCCT CGAAGTCTCC TTTTAAAAGG AAGTCTCACA GTCGTGAGAC ATCACACGAT 
GAAAACACAT CGTTTTTCCA CAAGAGAACA ATATCCGGTA GCAGTGCTCA CCATTCTAGA 
AACGTCAGTC AAGGTGCAGT ACCCTCTTCC GCACCACCTG TTTCTGGTGG AAATTATTCG 
CATAAGAGAA ACGTGTCGAG AGCTTCAAAT TCCTCTCAAA CTTCGAATTT TTTAGCCGAA 
CAATATGAAA GGGATAGGAA AGCCATAATT AATTGCTGCT TTTCCAGACC CGACCATAAG 
ACAGGTGAAC CGCCGAATAA TTATATTACA CATGTCCGGA TTATAGAAGA TTCAAAATTC 
CCTAGTTCAA GACCTCCTCC AGATTCAAAA TTGGAGAACA AAAAGAAAAG ACTCCTTATT 
TTGAGTGCCA AGCCCAACAA TGCCAAGCTA ATACAAATTC ATAAAGCAAG AGAAAACTCA 
GACGGCTCTT TTCAAATTGG TAGAACTTGG CAGTTGACCG AACTAGTAAG AGTAGAGAAG 
GACCTGGAGA TATCTGAAGG CTTCATTCTT ACCATGAGTA AAAAATACTA CTGGGAAACG 
AATTCTGCGA AGGAAAGAAC TGTTTTCATT AAATCTCTAA TTACTCTATA CATTCAAACA 
TTCGAAGGTC ATGTCCCTGA ATTGGTCAAT TGGGATTTAT CCCTTTTTTA TCTTGACGAG 
AGGAGTTATC AAAGAGCAGT CATCACAAAT CGCCCCGGTT CCGTATCTCC AATAAAGTCA 
CCTACAAGTA ATTTCACGAC AAACACTACC CAATCGGTGG GCTCTGTCCC CTTTTCAGCA 
CCTACTGAGA GGACAAGAAG GTCTGAAACA GAAAGCGTTA ATCCAGTGTC AACGCCTGCA 
AGCGTGGAAT ATCATGCAGG AATGAAATCT CTAAATAAGG CCCCCTATTC TTCCAATTCG 
ACATTGAATG AAGTGAATAA AAGATACGAG TTAGAGCAAC AACAGCAACA AGAAGAGGCT 
GAACTGAGAC GATTAGAAGA GCAAAAAAGA CTTCAGTTAC AAAAGGAAAA TGAGATGAAA 
AGATTAGAAG AGGAAAGAAG AATAAAGCAG GAGGAGAGGA AAAGGCAAAT GGAATTGGAA 
CACCAACGAC AACTCGAAGA AGAGGAACGA AAACGTCAAA TGGAACTGGA GGCTAAAAAG 
CAAATGGAGT TGAAACGTCA ACGGCAATTC GAAGAAGAGC AACGCCTAAA AAAAGAGAGA 
GAACTATTGG AAATACAGAG AAAACAAAGA GAACAAGAAA CAGCTGAGAG ATTAAAAAAA 
GAAGAGCAAG AAGCGCTTGC TAAAAAGGAG GAGGAAGAAA AAAGTAAAAG AAACAAAGTA 
GACAATGAAA GTTATACTCA AGAAATAAAT GGTAAGGTTG ATAACCTTTT GGAGGACTTA 
AACGCAGTTT TGGCGGAAGA AACAGAAACC ACCCCTACTA TGCAAAATGG TACTTACGTG 
CCCGAGAGAA GTACAGCTAG AGCTCATGAC CAATTAAAAA AACCACTAAA TATTGCCAAA 
GTTGAGTCAC TCGGTGGAAG CGATTTGAAT GACTCGATCA GTTTGAGTGA CGAGATAGCA 
GGTTTGAATA CATCTAACTT ATCAGGAGAG GACCAAGATG AGAAGAACGA TCTTTCGTTC 
GAGAAGGGAG ATGAGGTTAG GTATAGCAAT AATTTTGAAG GTGAAGCTCC TCACGTGTAC 
CATGAAGTGT CAATTATTCA AGAAGAAGCA CCTGCCGTTT CGCAGAAATT AATCCTTCCG 
GAAGAGAATA ACGAATCTGA AGCGCTGATT GAATCTAAAG AGGAGATAAA AACGATGGAA 
AATATTGACG ATGAGGTTTT ATTAGAAATA TTAACAGATA TCAATTGGTC CATTGAAGAC 
GATGCTGATA GTATGATTGA AAGGATTGAC TTAAGATTGG CGGAAACGGA ATACTTATTC 
AACCAAAATT TATTATCTTT GCAAAAAATT GGGCCTAATA TCCGCCCCTA CGAAGACAAG 
GTCAATGACG AATGTCATCG CATAATCCCC ACTCTCTCTT TATTCTTGAT GGAAATGAGC 
AATTTTTCTA ATGATATCGA AAACGTAGAG AGCCAAGATA ACGGATTACA AGTTGAAAGT 
GCTAACAAGA AACTTCTATG GAACACACTT GATGAACTTT TGAAAACTGT ATCTCTTGAT 
GAAATTTCCC TAAATCAGTT ATTAGAATGT CCAATAAGAG AGAAGAACTT GCCATGGATG 
GAAAACCAAT TGAATTTATT ATTAAAAGCA TTTCAAGCAA TAGGCAGCGA TGGAAATGAA 
GTAGAATATA ATCTAAGAGA AATTTCCGGA CTAAAGCAGA GGCTACAGTT TTATGAGAAA 
GTTACCAAGA TATTTCTTAA CAGAATTGTG GAGGAAATGC AGAAGAAATT CTCAAATATA 
CGTGGACAAG ATATCTCACA CGATCAAATG ATTAGAATTT TGACGACTTT ATTGATATTT 
TCTCCTTTAA TACTGTTCTG TAAAGAAATT TCACAAAAAT CGTATCAAGC AATTGTGGAA 
AATTGGAACG TCAGTATTCA ACCAGTATAC ATGGAGTTAT GGACTAAGAA AATATCACAA 
TTACAAGGCA TTGACACAAA TGATGAAAAA ATGAATGAGC TGAGCTTAAG CCAGTTACTA 
AATGAATGGG ACACATTTAG AAAAGAGAGA AAAACAAATG ATATCAACCC GGTTTTCAAG 
AATAGTTTTT CTCTTCTTAC CGAGTGCCTT CAAACCATGA GGCAAGAATG TATTGTTTAT 
CAAAATTTTG TCGAAGTGTT CTTTCATATA TCTTCAAAGC ATAATTTCGA AGAATACATT 
AAACATTTCA ATGATCCAGA TGCTCCTCCA ATATTATTAG ATACTGTGAA AGTAATGCAA 
TCTGATAGAG AAGCAGCCGT TATTGAAACC CAGTTGGTCT CAAGAATATT CCAACCTATT 
GTCACTAGGC TGTCCTCATA TTTTGTAGAA TTAGTGAAAG CCGAACCAAC GGTAGCCCCA 
GCCTTAACTT TTTACTTGGA GAATGAAATT AAAAGCTTAG AATCATCCAA CCATGAATTT 
TTATTGTCTG CAGTGACTAG AATGTACACA CAAATAAAAC AAGTGTGGTC TGATAATGTA 
GAAGAGCAGG TTTTGCACTT CGAAAGAATT TCTAACGCAA CCACCAATGG AGAAATTCTT 
CCAGGTATAC TTGATTTACC AGTAGGCTTG AAGAATTCAG AAGATTTGTT CCAATTTGCT 
AAGAGGTCTA TGGATATTAA AGATACCGAT GAAGGCTACG AGTCCATCGA ATTAATGAAT 
TCGAGCTTCA GGAAGCTAAG CATAGCTGCC ACACGATCGA TAACTCACAA AGAAGTTAAT 
TCAAGCATTA ATCCAAACCT TTCAGATACT GCTGCTTTAA ATAATGACTA TATGGAAACT 
ATTTCTCTCC TAGTGAACAG TAACTGGCTA ACTGAAATGC TTTCCATGTT AAACTTCAAT 
AAGGATGGTA TATTTGATAC GTCATTGCAA AACGTTAAGA AAGTTTTCGA TGTGGAAAAA 
GAATCTTATG CTTCCTTTCT ACTCCGCGAT ACTATGCCTA AACTAACAGC CTTTGTTTAT 
GGTGTGAGCA ACATCATTGA AAATACCAAC AATGTGAACA TGACCAATCC TTCAAGATGG 
GCCGCATATA GCAGGCAGAA CCTAGAGAAC ATTCTTTTAG CATACACTTC CCATGAAATT 
GAAACTCTAG TAAAAAGGCT GCACACTCAT ATGGTAAATG ACTTCGGTTA CCACCAAGAA 
AATGCCATAA ACAACGTCCT ATGCGATAAG TTGTGGTCGT GCATTCAAGG TCAAACCGTC 
TCACTATACT TGAAACTATA CACTGTAATT GATAAACACT ATAGAGGGAC AAACATACGC 
TTCACGAAGA ACGATATCAT AAGTGCGTTC GAGGAATACA AGAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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