PFA3 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Data show that for Pfa3 and its substrate Vac8, there are two consecutive steps in the acylation reaction: initial binding independent of cysteine in the DHHC box, but requiring myristoylation of Vac8, and a DHHC-motif dependent acylation.
Title: Analysis of DHHC acyltransferases implies overlapping substrate specificity and a two-step reaction mechanism.

Data describe a novel 16-amino-acid motif present at the cytosolic C-terminus of palmitoyltransferases, that is required for Swf1 and Pfa3 function in vivo.
Title: A novel motif at the C-terminus of palmitoyltransferases is essential for Swf1 and Pfa3 function in vivo.

The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.
Title: The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.

This study focusses on the function of PFA3 as the third member of the DHHC-CRD family found to have protein acyltransferase activity.
Title: The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p.