SEC24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC24
Entrez Gene ID	854697
Full Name	COPII subunit SEC24
Synonyms	ANU1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179457.3	NP_012157.3	COPII subunit SEC24

Pathways from BioSystems

Homologies

	SEC24B	XP_002804217.1
Canis lupus familiaris (dog)	SEC24B	XP_545021.2
Danio rerio (zebrafish)	wu:fa16f04	XP_001921634.3
Caenorhabditis elegans (roundworm)	sec-24.2	NP_502354.2
Kluyveromyces lactis	KLLA0F03729g	XP_455249.1
Schizosaccharomyces pombe (fission yeast)	sec24	NP_594731.1
Pan troglodytes (chimpanzee)	SEC24B	XP_001136519.3
Rattus norvegicus (Norway rat)	Sec24b	NP_001099944.1
Gallus gallus (chicken)	SEC24B	NP_001026306.1
Saccharomyces cerevisiae (baker's yeast)	SEC24	NP_012157.3
Eremothecium gossypii	AGOS_ADL239C	NP_983857.1
Xenopus tropicalis (tropical clawed frog)	sec24b	XP_002939332.2
Homo sapiens (human)	SEC24B	NP_006314.2
Bos taurus (cattle)	SEC24B	XP_005207729.1
Mus musculus (house mouse)	Sec24b	NP_997092.1
Magnaporthe oryzae (rice blast fungus)	MGG_09564	XP_003712177.1
Neurospora crassa	NCU02391	XP_959711.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here the authors show that phosphorylation of conserved amino acids on the membrane-distal surface of the Saccharomyces cerevisiae COPII cargo adaptor, Sec24, reprograms COPII vesicles for autophagy.
Title: Sec24 phosphorylation regulates autophagosome abundance during nutrient deprivation.

Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae.
Title: Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae.

Erv14 interaction with a novel cargo-binding surface on Sec24 is needed for efficient trafficking of all its clients. Some clients also engage an adjacent cargo-binding domain of Sec24, suggesting a novel mode of dual interaction between cargo and coat.
Title: Sec24 is a coincidence detector that simultaneously binds two signals to drive ER export.

the cytoplasmic N-terminal domain of Chs2p, which contains the CDK1 phosphorylation sites, interacts with the COPII component Sec24p in a yeast two-hybrid assay and that phosphomimetic substitutions of serines at the CDK1 consensus sites reduces the interaction.
Title: Phosphorylation of Chs2p regulates interaction with COPII.

association of Sec24p with Sec16p creates a regulatory complex that retards the GTPase activity of the COPII coat to prevent premature vesicle scission
Title: Sec24p and Sec16p cooperate to regulate the GTP cycle of the COPII coat.

The following SEC24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03733	NM_001179457.3 Latest version!	Saccharomyces cerevisiae S288C COPII subunit SEC24 (SEC24), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC24 ( NM_001179457.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012157.3 Saccharomyces cerevisiae S288C COPII subunit SEC24 (SEC24), partial mRNA.

Price & Availability↑

CloneID	OSi03733
Clone ID Related Accession (Same CDS sequence)	NM_001179457.3
Accession Version	NM_001179457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	COPII subunit SEC24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Jul 30, 2012 this sequence version replaced NM_001179457.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTCATC ACAAGAAACG TGTTTACCCA CAAGCTCAGC TCCAGTATGG ACAAAATGCC 
ACCCCTCTAC AACAGCCAGC ACAATTCATG CCTCCGCAGG ATCCTGCTGC AGCTGGAATG 
AGCTATGGTC AGATGGGAAT GCCGCCACAG GGTGCAGTCC CTTCCATGGG GCAGCAACAA 
TTCCTAACCC CTGCTCAAGA ACAGTTACAC CAGCAAATTG ATCAGGCCAC CACTTCCATG 
AACGATATGC ATCTGCACAA CGTACCGCTA GTCGATCCTA ACGCTTATAT GCAGCCACAG 
GTTCCCGTGC AAATGGGCAC ACCTTTGCAG CAACAGCAAC AACCAATGGC AGCGCCTGCG 
TATGGCCAAC CTAGCGCCGC AATGGGCCAA AACATGAGGC CCATGAACCA GTTGTATCCA 
ATCGATCTGT TGACGGAATT GCCTCCTCCA ATTACCGATC TGACCTTGCC TCCCCCACCG 
TTGGTTATTC CTCCAGAAAG GATGTTAGTT CCTTCTGAAT TGTCCAATGC GTCTCCAGAT 
TATATCAGGT CGACCTTGAA CGCCGTTCCT AAGAACAGCT CCCTGCTGAA GAAGTCTAAA 
CTTCCATTTG GGCTTGTTAT CAGACCATAC CAACACTTGT ACGACGATAT CGACCCGCCT 
CCATTGAATG AAGATGGACT TATTGTCCGT TGTCGTCGTT GTCGTTCTTA CATGAACCCT 
TTTGTCACTT TTATCGAGCA AGGCAGAAGA TGGAGATGTA ACTTTTGTCG TCTGGCCAAT 
GACGTTCCTA TGCAAATGGA CCAGAGCGAT CCAAACGACC CAAAGAGTAG ATACGACAGA 
AATGAAATCA AATGTGCTGT GATGGAATAT ATGGCCCCAA AGGAATATAC TCTAAGACAA 
CCACCTCCAG CCACTTACTG TTTCCTGATC GATGTCTCCC AGTCCTCTAT CAAGAGTGGG 
TTACTGGCCA CTACCATCAA CACCCTTTTG CAAAATCTTG ATTCCATTCC AAACCATGAT 
GAAAGAACCA GAATTTCCAT CTTATGTGTA GACAACGCCA TTCACTACTT TAAAATCCCA 
TTGGATTCTG AAAACAATGA AGAGTCCGCC GATCAAATCA ACATGATGGA TATTGCCGAC 
CTGGAAGAGC CTTTCTTGCC CAGACCAAAC TCTATGGTTG TGTCCTTAAA GGCCTGCAGA 
CAAAACATTG AAACTTTATT GACCAAGATT CCTCAAATCT TCCAATCTAA TTTAATCACA 
AATTTCGCAC TGGGCCCCGC TTTGAAATCC GCTTATCATT TAATTGGTGG CGTTGGTGGT 
AAAATAATTG TTGTGTCAGG TACTTTGCCC AACTTAGGCA TAGGAAAATT ACAAAGGAGA 
AATGAAAGTG GTGTTGTTAA TACATCCAAG GAAACTGCAC AACTACTTTC CTGTCAAGAC 
TCATTTTATA AGAATTTCAC CATCGATTGT TCTAAAGTAC AGATTACTGT CGATTTATTT 
TTGGCTTCTG AAGATTATAT GGACGTTGCA TCACTATCGA ATTTGAGTCG TTTCACTGCT 
GGTCAAACGC ATTTCTATCC TGGTTTTTCA GGCAAGAACC CAAATGATAT TGTCAAATTT 
TCCACTGAGT TTGCCAAGCA CATTTCCATG GATTTCTGTA TGGAAACCGT AATGAGAGCC 
CGTGGTTCCA CAGGTTTGAG AATGAGTAGA TTCTATGGTC ACTTCTTCAA TAGATCATCT 
GATTTATGTG CATTTTCCAC CATGCCAAGA GATCAATCAT ACTTATTCGA AGTTAACGTT 
GATGAATCCA TTATGGCTGA CTATTGTTAT GTTCAAGTAG CGGTACTTTT ATCTCTGAAT 
AATAGTCAAC GTAGAATAAG AATTATAACA TTGGCTATGC CAACCACGGA ATCGTTGGCA 
GAAGTCTACG CATCTGCTGA CCAATTAGCT ATAGCGTCGT TTTACAACTC GAAGGCGGTG 
GAGAAAGCAT TGAATTCCAG TTTGGACGAT GCAAGAGTAT TGATCAACAA ATCCGTGCAA 
GATATCTTAG CCACGTACAA GAAGGAAATA GTGGTGTCCA ACACTGCAGG GGGGGCCCCA 
TTGAGGTTAT GTGCCAATTT GAGAATGTTC CCATTGTTAA TGCATTCTTT AACTAAGCAT 
ATGGCATTTA GATCTGGTAT TGTCCCAAGT GACCATCGCG CCTCGGCCTT GAACAACTTA 
GAATCATTAC CTTTGAAATA TCTAATCAAA AACATCTACC CTGATGTTTA TTCGCTGCAT 
GATATGGCTG ATGAGGCTGG GTTACCAGTG CAAACTGAAG ATGGTGAAGC TACTGGCACA 
ATTGTTTTGC CTCAACCAAT CAATGCTACC TCTTCGTTGT TTGAAAGATA CGGTCTATAC 
TTGATAGATA ATGGTAACGA ATTGTTTTTG TGGATGGGTG GTGACGCTGT ACCAGCGCTA 
GTATTCGATG TGTTTGGAAC ACAGGATATC TTTGACATTC CAATTGGCAA ACAGGAAATA 
CCTGTTGTTG AAAATTCTGA ATTCAATCAA AGAGTAAGAA ACATTATCAA CCAATTGAGA 
AACCATGATG ATGTCATCAC TTACCAATCA TTGTATATTG TTAGGGGTGC CTCTTTGAGC 
GAGCCCGTTA ACCATGCATC TGCTAGGGAA GTTGCAACGC TAAGACTATG GGCTTCTAGC 
ACCTTAGTGG AGGATAAGAT TTTGAATAAC GAAAGTTACA GAGAATTCTT ACAAATCATG 
AAAGCCAGAA TTAGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012157.3
CDS	1..2781
Translation	MSHHKKRVYPQAQLQYGQNATPLQQPAQFMPPQDPAAAGMSYGQMGMPPQGAVPSMGQQQFLTPAQEQLHQQIDQATTSMNDMHLHNVPLVDPNAYMQPQVPVQMGTPLQQQQQPMAAPAYGQPSAAMGQNMRPMNQLYPIDLLTELPPPITDLTLPPPPLVIPPERMLVPSELSNASPDYIRSTLNAVPKNSSLLKKSKLPFGLVIRPYQHLYDDIDPPPLNEDGLIVRCRRCRSYMNPFVTFIEQGRRWRCNFCRLANDVPMQMDQSDPNDPKSRYDRNEIKCAVMEYMAPKEYTLRQPPPATYCFLIDVSQSSIKSGLLATTINTLLQNLDSIPNHDERTRISILCVDNAIHYFKIPLDSENNEESADQINMMDIADLEEPFLPRPNSMVVSLKACRQNIETLLTKIPQIFQSNLITNFALGPALKSAYHLIGGVGGKIIVVSGTLPNLGIGKLQRRNESGVVNTSKETAQLLSCQDSFYKNFTIDCSKVQITVDLFLASEDYMDVASLSNLSRFTAGQTHFYPGFSGKNPNDIVKFSTEFAKHISMDFCMETVMRARGSTGLRMSRFYGHFFNRSSDLCAFSTMPRDQSYLFEVNVDESIMADYCYVQVAVLLSLNNSQRRIRIITLAMPTTESLAEVYASADQLAIASFYNSKAVEKALNSSLDDARVLINKSVQDILATYKKEIVVSNTAGGAPLRLCANLRMFPLLMHSLTKHMAFRSGIVPSDHRASALNNLESLPLKYLIKNIYPDVYSLHDMADEAGLPVQTEDGEATGTIVLPQPINATSSLFERYGLYLIDNGNELFLWMGGDAVPALVFDVFGTQDIFDIPIGKQEIPVVENSEFNQRVRNIINQLRNHDDVITYQSLYIVRGASLSEPVNHASAREVATLRLWASSTLVEDKILNNESYREFLQIMKARISK

Target ORF information:

RefSeq Version	NM_001179457.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C COPII subunit SEC24 (SEC24), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179457.3

ORF Insert Sequence:

ATGTCTCATC ACAAGAAACG TGTTTACCCA CAAGCTCAGC TCCAGTATGG ACAAAATGCC 
ACCCCTCTAC AACAGCCAGC ACAATTCATG CCTCCGCAGG ATCCTGCTGC AGCTGGAATG 
AGCTATGGTC AGATGGGAAT GCCGCCACAG GGTGCAGTCC CTTCCATGGG GCAGCAACAA 
TTCCTAACCC CTGCTCAAGA ACAGTTACAC CAGCAAATTG ATCAGGCCAC CACTTCCATG 
AACGATATGC ATCTGCACAA CGTACCGCTA GTCGATCCTA ACGCTTATAT GCAGCCACAG 
GTTCCCGTGC AAATGGGCAC ACCTTTGCAG CAACAGCAAC AACCAATGGC AGCGCCTGCG 
TATGGCCAAC CTAGCGCCGC AATGGGCCAA AACATGAGGC CCATGAACCA GTTGTATCCA 
ATCGATCTGT TGACGGAATT GCCTCCTCCA ATTACCGATC TGACCTTGCC TCCCCCACCG 
TTGGTTATTC CTCCAGAAAG GATGTTAGTT CCTTCTGAAT TGTCCAATGC GTCTCCAGAT 
TATATCAGGT CGACCTTGAA CGCCGTTCCT AAGAACAGCT CCCTGCTGAA GAAGTCTAAA 
CTTCCATTTG GGCTTGTTAT CAGACCATAC CAACACTTGT ACGACGATAT CGACCCGCCT 
CCATTGAATG AAGATGGACT TATTGTCCGT TGTCGTCGTT GTCGTTCTTA CATGAACCCT 
TTTGTCACTT TTATCGAGCA AGGCAGAAGA TGGAGATGTA ACTTTTGTCG TCTGGCCAAT 
GACGTTCCTA TGCAAATGGA CCAGAGCGAT CCAAACGACC CAAAGAGTAG ATACGACAGA 
AATGAAATCA AATGTGCTGT GATGGAATAT ATGGCCCCAA AGGAATATAC TCTAAGACAA 
CCACCTCCAG CCACTTACTG TTTCCTGATC GATGTCTCCC AGTCCTCTAT CAAGAGTGGG 
TTACTGGCCA CTACCATCAA CACCCTTTTG CAAAATCTTG ATTCCATTCC AAACCATGAT 
GAAAGAACCA GAATTTCCAT CTTATGTGTA GACAACGCCA TTCACTACTT TAAAATCCCA 
TTGGATTCTG AAAACAATGA AGAGTCCGCC GATCAAATCA ACATGATGGA TATTGCCGAC 
CTGGAAGAGC CTTTCTTGCC CAGACCAAAC TCTATGGTTG TGTCCTTAAA GGCCTGCAGA 
CAAAACATTG AAACTTTATT GACCAAGATT CCTCAAATCT TCCAATCTAA TTTAATCACA 
AATTTCGCAC TGGGCCCCGC TTTGAAATCC GCTTATCATT TAATTGGTGG CGTTGGTGGT 
AAAATAATTG TTGTGTCAGG TACTTTGCCC AACTTAGGCA TAGGAAAATT ACAAAGGAGA 
AATGAAAGTG GTGTTGTTAA TACATCCAAG GAAACTGCAC AACTACTTTC CTGTCAAGAC 
TCATTTTATA AGAATTTCAC CATCGATTGT TCTAAAGTAC AGATTACTGT CGATTTATTT 
TTGGCTTCTG AAGATTATAT GGACGTTGCA TCACTATCGA ATTTGAGTCG TTTCACTGCT 
GGTCAAACGC ATTTCTATCC TGGTTTTTCA GGCAAGAACC CAAATGATAT TGTCAAATTT 
TCCACTGAGT TTGCCAAGCA CATTTCCATG GATTTCTGTA TGGAAACCGT AATGAGAGCC 
CGTGGTTCCA CAGGTTTGAG AATGAGTAGA TTCTATGGTC ACTTCTTCAA TAGATCATCT 
GATTTATGTG CATTTTCCAC CATGCCAAGA GATCAATCAT ACTTATTCGA AGTTAACGTT 
GATGAATCCA TTATGGCTGA CTATTGTTAT GTTCAAGTAG CGGTACTTTT ATCTCTGAAT 
AATAGTCAAC GTAGAATAAG AATTATAACA TTGGCTATGC CAACCACGGA ATCGTTGGCA 
GAAGTCTACG CATCTGCTGA CCAATTAGCT ATAGCGTCGT TTTACAACTC GAAGGCGGTG 
GAGAAAGCAT TGAATTCCAG TTTGGACGAT GCAAGAGTAT TGATCAACAA ATCCGTGCAA 
GATATCTTAG CCACGTACAA GAAGGAAATA GTGGTGTCCA ACACTGCAGG GGGGGCCCCA 
TTGAGGTTAT GTGCCAATTT GAGAATGTTC CCATTGTTAA TGCATTCTTT AACTAAGCAT 
ATGGCATTTA GATCTGGTAT TGTCCCAAGT GACCATCGCG CCTCGGCCTT GAACAACTTA 
GAATCATTAC CTTTGAAATA TCTAATCAAA AACATCTACC CTGATGTTTA TTCGCTGCAT 
GATATGGCTG ATGAGGCTGG GTTACCAGTG CAAACTGAAG ATGGTGAAGC TACTGGCACA 
ATTGTTTTGC CTCAACCAAT CAATGCTACC TCTTCGTTGT TTGAAAGATA CGGTCTATAC 
TTGATAGATA ATGGTAACGA ATTGTTTTTG TGGATGGGTG GTGACGCTGT ACCAGCGCTA 
GTATTCGATG TGTTTGGAAC ACAGGATATC TTTGACATTC CAATTGGCAA ACAGGAAATA 
CCTGTTGTTG AAAATTCTGA ATTCAATCAA AGAGTAAGAA ACATTATCAA CCAATTGAGA 
AACCATGATG ATGTCATCAC TTACCAATCA TTGTATATTG TTAGGGGTGC CTCTTTGAGC 
GAGCCCGTTA ACCATGCATC TGCTAGGGAA GTTGCAACGC TAAGACTATG GGCTTCTAGC 
ACCTTAGTGG AGGATAAGAT TTTGAATAAC GAAAGTTACA GAGAATTCTT ACAAATCATG 
AAAGCCAGAA TTAGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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