Gene Symbol | PRP5 |
Entrez Gene ID | 852539 |
Full Name | DEAD-box RNA helicase PRP5 |
Synonyms | RNA5 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » PRP5 cDNA ORF clone
Gene Symbol | PRP5 |
Entrez Gene ID | 852539 |
Full Name | DEAD-box RNA helicase PRP5 |
Synonyms | RNA5 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001178585.1 | NP_009796.1 | DEAD-box RNA helicase PRP5 |
Saccharomyces cerevisiae (baker's yeast) | PRP5 | NP_009796.1 |
Eremothecium gossypii | AGOS_AFL027C | NP_985521.2 |
Kluyveromyces lactis | KLLA0F10505g | XP_455558.1 |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
Taken together, our results indicate that the Psis in U2 snRNA contribute to pre-mRNA splicing by directly altering the binding/ATPase activity of Prp5.
Title: Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly.
Prp5 binds to the spliceosome in association with U2 by interacting with the BSL and is released upon the base-pairing of U2 with the branch site to allow the recruitment of the tri-snRNP.
Title: A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.
The following PRP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi01933 | |
Clone ID Related Accession (Same CDS sequence) | NM_001178585.1 | |
Accession Version | NM_001178585.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | DEAD-box RNA helicase PRP5 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGGAAACTA TTGATTCGAA GCAAAATATT AATAGGGAGT CTTTATTGGA GGAAAGGAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_009796.1 |
CDS | 1..2550 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001178585.1 |
1 | ATGGAAACTA TTGATTCGAA GCAAAATATT AATAGGGAGT CTTTATTGGA GGAAAGGAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
Complete DNA sequence of yeast chromosome II. |