GLE1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GLE1
Entrez Gene ID	851320
Full Name	nucleoporin GLE1
Synonyms	BRR3,NLE2,RSS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180267.1	NP_010074.1	nucleoporin GLE1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C15235g	XP_452883.1
Saccharomyces cerevisiae (baker's yeast)	GLE1	NP_010074.1
Schizosaccharomyces pombe (fission yeast)	gle1	NP_595101.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation.
Title: Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation.

Gle1 specifically binds IP(6); this interaction is required for the full potentiation of Dbp5 ATPase activity during both mRNA export and translation termination
Title: Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle1.

Study identified several charged surface residues that, when mutated, weaken the binding of Gle1 and inhibit the ability of Gle1 to stimulate Dbp5's ATPase activity.
Title: Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1.

Study shows that Gle1 and inositol hexakisphosphate are required for efficient translation termination, in addition, Gle1 has a conserved physical association with the initiation factor eIF3.
Title: The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation.

Our results define specific functions for Gle1 and InsP6 in mRNA export and suggest that local activation of Dbp5 at the nuclear pore is critical for mRNA export.
Title: Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export.

The following GLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00883	NM_001180267.1 Latest version!	Saccharomyces cerevisiae S288C nucleoporin GLE1 (GLE1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLE1 ( NM_001180267.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010074.1 Saccharomyces cerevisiae S288C nucleoporin GLE1 (GLE1), partial mRNA.

Price & Availability↑

CloneID	OSi00883
Clone ID Related Accession (Same CDS sequence)	NM_001180267.1
Accession Version	NM_001180267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	nucleoporin GLE1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGATTTG TGTTCGATGA GGTTTTCAAT TCAGATACTG ATTCTCCAGA ATTTGAGGAA 
ACATGTTCTA CAACATCTTC TACGTCATCG CAATGCCCTA CACCTGAGCC GAGCCCAGCA 
ATAAAGTTAC CTTCATTCAC GAAAGTTGGC ACGAAGAAAT TGGTAAATGA GTCGGTAGTT 
ATTTTAGATC CTGCTCTAGA AAACGCCTTG CGCGATTTGA ACCTTCAATC GAAACTGATC 
CCCATTAATG AGCCCATTGT AGCCGCGTCA TCGATAATAG TACCGCATTC AACCAATATG 
CCACTACCGA GAGCATCGCA TTCGTCATTG CTAGATAATG CTAAGAACTC AAATGCGACA 
GCACCCTTAC TCGAGGCAAT AGAAGAATCT TTTCAAAGGA AAATGCAAAA CTTGGTGCTT 
GCCAATCAGA AAGAAATTCA ATCTATTAGA GAAAATAAAC GTCGTGTTGA AGAGCAACGT 
AAGCGAAAAG AGGAGGAAGA GCGCAAACGT AAGGAAGCTG AGGAAAAGGC CAAGCGGGAG 
CAGGAATTAT TACGTCAAAA GAAGGATGAA GAAGAACGTA AACGCAAAGA AGCTGAAGCA 
AAGCTAGCGC AGCAGAAACA AGAAGAAGAA AGGAAGAAAA TAGAAGAACA GAATGAAAAG 
GAAAGACAAT TGAAAAAAGA ACATGAAGCC AAATTATTGC AGCAGAAGGA TAAATTGGGT 
AAAGCAGTTA CAAACTTTGA TAAAATCTCC AAAATGTTTT GGCATTATAA AGACAAAATT 
GCTCAAATAA AGCAAGACAT AGTTCTGCCT ATAAAAAAAG CTGATGTGAA TGTAAGGAAT 
TTGCTCTCAC GTCATAAAAG GAAAATTAAC CCGAAATTCG GACAATTAAC TAATAGCAAC 
CAGCAACTTT TTAAAATACA GAATGAACTA ACTCAATTGA TAAATGATAC TAAAGGCGAC 
TCTCTAGCAT ATCATTGGAT TTTAAACTTT ATTGCCAAAG CCGTAGTACA TCAGGCGGAA 
ACAGAAGTCA GGGTAAAACC AGAGTCTGCT CTGCCCCTAG GGAAATTGAC TTTATATTTG 
TTAGTCCAAT TCCCAGAGTT ACAGGAATTG TTTATGGCAA GACTTGTCAA GAAATGCCCC 
TTTGTGATAG GATTCACGTG TGAGATTGAC ACAGAAAAAG GCCGTCAAAA CATGGGATGG 
AAAAGAAACA ACGAGAATAA ATGGGAAGAT AACACATCCT ACGATGAGAG AATGGGCGGG 
ATCTTATCAT TATTTGCCAT AATAACGAGA TTACAGTTAC CTCAAGAATT TATCACCACC 
ACGAGTCATC CTTTCCCCAT CGCATTATCG TGGCATATTT TGGCTAGAAT ATGTAATACG 
CCACTAAATT TAATAACAAA TACCCATTTT GTCATACTCG GTTCGTGGTG GGATGCCGCT 
GCCGTACAGT TTCTTCAGGC GTATGGGAAC CAAGCCTCTA AATTGCTAAT CTTGATTGGT 
GAGGAATTGA CCTCCAGAAT GGCTGAAAAG AAATATGTGG GTGCTGCGAG ATTAAGAATT 
TTATTAGAGG CCTGGCAAAA TAATAATATG GAGTCCTTTC CGGAAATGTC TCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010074.1
CDS	1..1617
Translation	MRFVFDEVFNSDTDSPEFEETCSTTSSTSSQCPTPEPSPAIKLPSFTKVGTKKLVNESVVILDPALENALRDLNLQSKLIPINEPIVAASSIIVPHSTNMPLPRASHSSLLDNAKNSNATAPLLEAIEESFQRKMQNLVLANQKEIQSIRENKRRVEEQRKRKEEEERKRKEAEEKAKREQELLRQKKDEEERKRKEAEAKLAQQKQEEERKKIEEQNEKERQLKKEHEAKLLQQKDKLGKAVTNFDKISKMFWHYKDKIAQIKQDIVLPIKKADVNVRNLLSRHKRKINPKFGQLTNSNQQLFKIQNELTQLINDTKGDSLAYHWILNFIAKAVVHQAETEVRVKPESALPLGKLTLYLLVQFPELQELFMARLVKKCPFVIGFTCEIDTEKGRQNMGWKRNNENKWEDNTSYDERMGGILSLFAIITRLQLPQEFITTTSHPFPIALSWHILARICNTPLNLITNTHFVILGSWWDAAAVQFLQAYGNQASKLLILIGEELTSRMAEKKYVGAARLRILLEAWQNNNMESFPEMSP

Target ORF information:

RefSeq Version	NM_001180267.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C nucleoporin GLE1 (GLE1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180267.1

ORF Insert Sequence:

ATGAGATTTG TGTTCGATGA GGTTTTCAAT TCAGATACTG ATTCTCCAGA ATTTGAGGAA 
ACATGTTCTA CAACATCTTC TACGTCATCG CAATGCCCTA CACCTGAGCC GAGCCCAGCA 
ATAAAGTTAC CTTCATTCAC GAAAGTTGGC ACGAAGAAAT TGGTAAATGA GTCGGTAGTT 
ATTTTAGATC CTGCTCTAGA AAACGCCTTG CGCGATTTGA ACCTTCAATC GAAACTGATC 
CCCATTAATG AGCCCATTGT AGCCGCGTCA TCGATAATAG TACCGCATTC AACCAATATG 
CCACTACCGA GAGCATCGCA TTCGTCATTG CTAGATAATG CTAAGAACTC AAATGCGACA 
GCACCCTTAC TCGAGGCAAT AGAAGAATCT TTTCAAAGGA AAATGCAAAA CTTGGTGCTT 
GCCAATCAGA AAGAAATTCA ATCTATTAGA GAAAATAAAC GTCGTGTTGA AGAGCAACGT 
AAGCGAAAAG AGGAGGAAGA GCGCAAACGT AAGGAAGCTG AGGAAAAGGC CAAGCGGGAG 
CAGGAATTAT TACGTCAAAA GAAGGATGAA GAAGAACGTA AACGCAAAGA AGCTGAAGCA 
AAGCTAGCGC AGCAGAAACA AGAAGAAGAA AGGAAGAAAA TAGAAGAACA GAATGAAAAG 
GAAAGACAAT TGAAAAAAGA ACATGAAGCC AAATTATTGC AGCAGAAGGA TAAATTGGGT 
AAAGCAGTTA CAAACTTTGA TAAAATCTCC AAAATGTTTT GGCATTATAA AGACAAAATT 
GCTCAAATAA AGCAAGACAT AGTTCTGCCT ATAAAAAAAG CTGATGTGAA TGTAAGGAAT 
TTGCTCTCAC GTCATAAAAG GAAAATTAAC CCGAAATTCG GACAATTAAC TAATAGCAAC 
CAGCAACTTT TTAAAATACA GAATGAACTA ACTCAATTGA TAAATGATAC TAAAGGCGAC 
TCTCTAGCAT ATCATTGGAT TTTAAACTTT ATTGCCAAAG CCGTAGTACA TCAGGCGGAA 
ACAGAAGTCA GGGTAAAACC AGAGTCTGCT CTGCCCCTAG GGAAATTGAC TTTATATTTG 
TTAGTCCAAT TCCCAGAGTT ACAGGAATTG TTTATGGCAA GACTTGTCAA GAAATGCCCC 
TTTGTGATAG GATTCACGTG TGAGATTGAC ACAGAAAAAG GCCGTCAAAA CATGGGATGG 
AAAAGAAACA ACGAGAATAA ATGGGAAGAT AACACATCCT ACGATGAGAG AATGGGCGGG 
ATCTTATCAT TATTTGCCAT AATAACGAGA TTACAGTTAC CTCAAGAATT TATCACCACC 
ACGAGTCATC CTTTCCCCAT CGCATTATCG TGGCATATTT TGGCTAGAAT ATGTAATACG 
CCACTAAATT TAATAACAAA TACCCATTTT GTCATACTCG GTTCGTGGTG GGATGCCGCT 
GCCGTACAGT TTCTTCAGGC GTATGGGAAC CAAGCCTCTA AATTGCTAAT CTTGATTGGT 
GAGGAATTGA CCTCCAGAAT GGCTGAAAAG AAATATGTGG GTGCTGCGAG ATTAAGAATT 
TTATTAGAGG CCTGGCAAAA TAATAATATG GAGTCCTTTC CGGAAATGTC TCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GLE1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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