MYO4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO4
Entrez Gene ID	851204
Full Name	myosin 4
Synonyms	FUN22,SHE1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178174.1	NP_009373.1	myosin 4

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MYO4	NP_009373.1
Arabidopsis thaliana (thale cress)	XIJ	NP_191375.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells.
Title: Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.

In Saccharomyces cerevisiae, the MyoV motor Myo4p interacts with She3p to localize asymmetric synthesis of HO 1 (ASH1) mRNA into the bud of dividing cells
Title: The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.

multiple, nonprocessive Myo4 motors can generate continuous transport of mRNA to the bud tip
Title: Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.

The most important feature for correct localization is the retention of coupling between all the members of the complex (Myo4p-She3p-She2p-ASH1 mRNA), which is aided by She3p being a tightly bound subunit of Myo4p.
Title: Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.

continuous transport of mRNA by Myo4p-She3p in yeast is accomplished either by multiple high duty cycle monomers or by molecules that may be dimerized by She2p, the homodimeric downstream binding partner of She3p
Title: She3p binds to the rod of yeast myosin V and prevents it from dimerizing, forming a single-headed motor complex.

The following MYO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00790	NM_001178174.1 Latest version!	Saccharomyces cerevisiae S288C myosin 4 (MYO4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO4 ( NM_001178174.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009373.1 Saccharomyces cerevisiae S288C myosin 4 (MYO4), partial mRNA.

Price & Availability↑

CloneID	OSi00790
Clone ID Related Accession (Same CDS sequence)	NM_001178174.1
Accession Version	NM_001178174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	myosin 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATTTG AAGTAGGAAC TAAGTGTTGG TACCCTCACA AAGAACAAGG CTGGATAGGC 
GGCGAAGTCA CCAAGAATGA CTTCTTTGAA GGAACGTTCC ACTTAGAGTT GAAATTGGAG 
GATGGAGAAA CTGTATCCAT TGAAACAAAT AGCTTCGAAA ATGATGATGA TCATCCCACG 
CTACCTGTAC TACGAAACCC ACCTATTTTA GAGTCTACTG ATGACTTAAC CACCCTATCA 
TATCTGAACG AACCGGCAGT GCTGCATGCC ATAAAAAAAA GATACATGAA TGGACAGATA 
TATACTTACT CTGGTATTGT CCTTATTGCC GCTAATCCCT TTGATAAAGT CGACCATTTG 
TACTCTCGCG AGATGATACA GAATTATTCT AGTAAGCGTA AAGATGAGCT GGAGCCGCAC 
CTTTTTGCCA TAGCAGAGGA GGCGTACAGG TTCATGGTCC ATGAAAAGGC TAACCAGACT 
GTTGTAGTCA GTGGTGAATC CGGTGCTGGT AAGACCGTCT CTGCTAAATA CATTATGAGA 
TACTTTGCCT CAGTCCAAGA AAGCAACAAT CGTGAAGGGG AAGTGGAAAT GTCTCAAATC 
GAGAGCCAAA TTTTAGCTAC TAACCCGATC ATGGAAGCCT TTGGTAACGC CAAAACTACC 
AGAAATGATA ATTCCTCTCG TTTCGGCAAA TATCTGCAAA TTTTATTTGA TGAGAATACT 
ACCATCAGGG GGTCCAAAAT TAGAACCTAT CTCTTGGAAA AATCCAGGTT AGTTTATCAG 
CCAGAGACAG AAAGAAATTA CCATATTTTT TACCAAATTT TGGAAGGATT GCCAGAACCG 
GTAAAACAAG AACTGCATTT GTCATCTCCC AAGGACTACC ATTATACTAA CCAAGGTGGA 
CAGCCGAACA TAGCTGGTAT AGATGAAGCT CGAGAATACA AGATAACCAC TGATGCCCTG 
TCATTAGTAG GTATAAACCA CGAGACTCAG CTTGGAATTT TCAAAATTCT TGCAGGCTTG 
CTACATATAG GTAATATCGA GATGAAAATG ACTAGAAACG ACGCATCACT ATCATCGGAA 
GAGCAAAACC TGCAAATCGC ATGTGAATTA TTGGGTATTG ATCCCTTCAA TTTTGCAAAA 
TGGATTGTCA AGAAACAAAT CGTCACAAGG TCTGAAAAAA TTGTCACTAA TTTGAATTAC 
AATCAAGCGC TAATTGCTAG GGACTCTGTG GCGAAATTTA TTTATTCCAC GCTTTTTGAT 
TGGCTAGTAG ACAACATTAA CAAGACATTA TACGATCCTG AGCTGGATCA ACAAGATCAT 
GTCTTTTCCT TTATTGGTAT TTTAGATATC TACGGGTTTG AGCATTTTGA GAAAAACTCA 
TTCGAACAAT TCTGTATAAA CTATGCGAAT GAAAAATTAC AACAAGAGTT CAACCAGCAC 
GTTTTCAAAT TGGAGCAAGA AGAATATGTA AAGGAGGAAA TTGAGTGGTC TTTCATTGAG 
TTCAGCGACA ACCAACCTTG CATAGACTTG ATTGAGAATA AACTTGGAAT ACTTTCTCTA 
TTAGATGAAG AAAGTAGATT ACCATCAGGC TCAGATGAAT CATGGGCCTC AAAACTATAT 
TCTGCCTTTA ATAAACCGCC CTCAAATGAA GTTTTTTCGA AGCCAAGATT CGGACAAACG 
AAGTTTATAG TAAGCCATTA TGCTGTTGAT GTAGAATATG AAGTTGAAGG GTTTATTGAA 
AAGAATAGAG ACAGCGTTTC CCTAGGTCAT CTGGATGTTT TCAAAGCAAC GACAAATCCA 
ATTTTCAAAC AAATTTTAGA CAACAGAGAA CTTAGGAGTG ACGATGCTCC TGAAGAGCAA 
AATACTGAAA AAAAGATAAT GATCCCAGCA AGATTAAGTC AAAAGAAACC AACCCTGGGA 
TCTATGTTCA AAAAATCGCT CGGGGAATTG ATGGCCATCA TAAATTCTAC TAATGTTCAT 
TATATTCGTT GCATAAAACC AAATTCTGAA AAAAAGCCAT GGGAGTTCGA TAATTTGATG 
GTCTTATCGC AATTAAGAGC TTGTGGTGTG CTGGAAACAA TCAGGATTTC ATGTGCAGGC 
TTTCCATCAA GATGGACTTT TGACGAATTC GTTCAAAGAT ATTTCCTCCT GACGGACTAC 
AGCTTATGGA GTGGAATTCT TTATAATCCA GACCTCCCAA AAGAGGCAAT CGTCAACTTC 
TGTCAATCTA TTCTAGATGC TACTATCTCT GATTCAGCAA AATACCAAAT TGGTAATACC 
AAAATTTTCT TTAAAGCAGG AATGCTTGCA TTTTTGGAAA AGCTAAGGAC TAATAAAATG 
AATGAAATAT GCATCATAAT ACAGAAAAAG ATAAGGGCAA GATACTATAG GCTTCAGTAT 
TTGCAGACCA TGGAATCAAT AAAGAAGTGC CAAAGCCAAA TTAGAAGTTT GTTGGTTCGT 
ACAAGGGTCG ATCATGAGCT CAAGACAAGA GCTGCCATAT TACTACAAAC GAACATTAGA 
GCATTATGGA AACGGGAATA CTATAGAGCT GCCATTGGGC AAATAATAAA ACTACAATGT 
ACCTGTAAAA GAAAATTGAT TTTAGATAGT GTTAACAGGA AATTCATGCT CATGGCGGCA 
GTAATTATCC AAAGCTACAT CAGATCATAT GGTCACAAAA CAGATTACAG AACATTGAAA 
AGGAGCTCCA TTCTTGTACA AAGCGCCATG AGAATGCAGT TAGCTAGACG AAGGTATATA 
GTTCTGCAAA AAGAAGTTGA AGAACGGAAT ATTAGGGCTA GTTACGGGAT TGGATTACTG 
GAGGAAGCTA TCGAATTTAA GAACTCTTTC ATTTTGAATC TAGAGATGTT AAATGACTCA 
TATACAAGAT TAACACAGTT GTTGCAGGGA GATTTGTCAA ATATTCCATC TAAGCAAAGG 
CAAGAGTACG AAACGATAGT AAATGGTTAT AATGATAAGA TTTCCAAGCT GAAGACGTTA 
CAAGTTGAAA TCATGAATAC TTTAAACAAG AAGAATGCCT TGAAGGAGAG AAAAAAGAAG 
CAATCTTCTT TAATACAGTC ACATATGCAA AGTTTGGCTG CTATAAAAGG TAACAAACCT 
TCAAGATTAA GTGATGAAGT CAAATCAATG AAGCAAGAAC TAGCGTTTAT TGAAAACGTA 
ATAGCACAAG ATTTCACCAC CACATATTCT GCTAATAAGA ATGATAAAGT GAAAGGTTTG 
GGAATCGCAG GACAACAAGT AAAACCGAAG CTAGTCAATG TAATTCGTAG AGAATCAGGA 
AATCCTGATC TTCTAGAATT ACTAATGGAC CTGAATTGTT ACACATTAGA AGTCACAGAA 
GGTTACTTGA AAAAAGTAAA TGTTACTGAG GTGAATGGAG ACAATGTTCT GGGTCCTATA 
CACGTCATTA CTACAGTGGT AAGTAGCTTA GTGAGAAATG GGTTATTGAT TCAAAGTAGC 
AAATTCATCT CGAAAGTCCT ATTGACTGTA GAAAGCATTG TTATGAGTCT ACCAAAGGAC 
GAAACAATGC TTGGTGGTAT CTTTTGGTTA AGTAATCTTT CCAGACTTCC CGCATTTGCT 
GCAAATCAAA AAACATTATA TGAAGCGAAT GGTGGTGATG AAAAAGATAA GTTAACACTG 
ATATATTTGA ATGATTTGGA GAATGAAACT TTAAAAGTAT TCGACAAGAT TTATTCCACT 
TGGTTAGTGA AATTCATGAA GCATGCTTCA GCCCACATCG AGATCTTCGA TATGGTTTTA 
AACGAGAAGC TATTTAAAAA TTCTGGTGAT GAGAAGTTCG CTAAATTATT CACATTCTTA 
AATGAATTTG ATGCTGTCTT GTGTAAATTT CAAGTTGTTG ACTCAATGCA TACAAAAATT 
TTTAACGACA CCCTGAAGTA TCTGAACGTA ATGCTATTTA ATGACCTAAT AACCAAATGT 
CCCGCGTTAA ATTGGAAGTA TGGGTACGAA GTGGATAGAA ATATCGAGCG GTTGGTGAGC 
TGGTTTGAAC CAAGAATTGA AGACGTTCGT CCCAATTTGA TTCAAATAAT TCAGGCTGTC 
AAGATATTAC AGTTGAAGAT AAGCAACTTG AACGAGTTCA AGTTACTATT CGATTTCTGG 
TATGCCTTGA ATCCAGCCCA AATACAAGCA ATTCTGTTGA AATACAAGCC TGCTAACAAG 
GGCGAAGCTG GAGTACCGAA CGAAATCTTG AATTATTTAG CTAACGTGAT CAAAAGAGAA 
AATCTGTCTT TGCCAGGGAA GATGGAAATT ATGCTTTCTG CCCAATTTGA CTCTGCTAAA 
AACCATCTGC GTTATGACAC TAGTGCAATA ACACAGAACA GCAATACAGA GGGCTTAGCT 
ACTGTCAGTA AAATTATAAA ATTAGACAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009373.1
CDS	1..4416
Translation	MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVKPKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQKTLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKCPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK

Target ORF information:

RefSeq Version	NM_001178174.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C myosin 4 (MYO4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178174.1

ORF Insert Sequence:

ATGTCATTTG AAGTAGGAAC TAAGTGTTGG TACCCTCACA AAGAACAAGG CTGGATAGGC 
GGCGAAGTCA CCAAGAATGA CTTCTTTGAA GGAACGTTCC ACTTAGAGTT GAAATTGGAG 
GATGGAGAAA CTGTATCCAT TGAAACAAAT AGCTTCGAAA ATGATGATGA TCATCCCACG 
CTACCTGTAC TACGAAACCC ACCTATTTTA GAGTCTACTG ATGACTTAAC CACCCTATCA 
TATCTGAACG AACCGGCAGT GCTGCATGCC ATAAAAAAAA GATACATGAA TGGACAGATA 
TATACTTACT CTGGTATTGT CCTTATTGCC GCTAATCCCT TTGATAAAGT CGACCATTTG 
TACTCTCGCG AGATGATACA GAATTATTCT AGTAAGCGTA AAGATGAGCT GGAGCCGCAC 
CTTTTTGCCA TAGCAGAGGA GGCGTACAGG TTCATGGTCC ATGAAAAGGC TAACCAGACT 
GTTGTAGTCA GTGGTGAATC CGGTGCTGGT AAGACCGTCT CTGCTAAATA CATTATGAGA 
TACTTTGCCT CAGTCCAAGA AAGCAACAAT CGTGAAGGGG AAGTGGAAAT GTCTCAAATC 
GAGAGCCAAA TTTTAGCTAC TAACCCGATC ATGGAAGCCT TTGGTAACGC CAAAACTACC 
AGAAATGATA ATTCCTCTCG TTTCGGCAAA TATCTGCAAA TTTTATTTGA TGAGAATACT 
ACCATCAGGG GGTCCAAAAT TAGAACCTAT CTCTTGGAAA AATCCAGGTT AGTTTATCAG 
CCAGAGACAG AAAGAAATTA CCATATTTTT TACCAAATTT TGGAAGGATT GCCAGAACCG 
GTAAAACAAG AACTGCATTT GTCATCTCCC AAGGACTACC ATTATACTAA CCAAGGTGGA 
CAGCCGAACA TAGCTGGTAT AGATGAAGCT CGAGAATACA AGATAACCAC TGATGCCCTG 
TCATTAGTAG GTATAAACCA CGAGACTCAG CTTGGAATTT TCAAAATTCT TGCAGGCTTG 
CTACATATAG GTAATATCGA GATGAAAATG ACTAGAAACG ACGCATCACT ATCATCGGAA 
GAGCAAAACC TGCAAATCGC ATGTGAATTA TTGGGTATTG ATCCCTTCAA TTTTGCAAAA 
TGGATTGTCA AGAAACAAAT CGTCACAAGG TCTGAAAAAA TTGTCACTAA TTTGAATTAC 
AATCAAGCGC TAATTGCTAG GGACTCTGTG GCGAAATTTA TTTATTCCAC GCTTTTTGAT 
TGGCTAGTAG ACAACATTAA CAAGACATTA TACGATCCTG AGCTGGATCA ACAAGATCAT 
GTCTTTTCCT TTATTGGTAT TTTAGATATC TACGGGTTTG AGCATTTTGA GAAAAACTCA 
TTCGAACAAT TCTGTATAAA CTATGCGAAT GAAAAATTAC AACAAGAGTT CAACCAGCAC 
GTTTTCAAAT TGGAGCAAGA AGAATATGTA AAGGAGGAAA TTGAGTGGTC TTTCATTGAG 
TTCAGCGACA ACCAACCTTG CATAGACTTG ATTGAGAATA AACTTGGAAT ACTTTCTCTA 
TTAGATGAAG AAAGTAGATT ACCATCAGGC TCAGATGAAT CATGGGCCTC AAAACTATAT 
TCTGCCTTTA ATAAACCGCC CTCAAATGAA GTTTTTTCGA AGCCAAGATT CGGACAAACG 
AAGTTTATAG TAAGCCATTA TGCTGTTGAT GTAGAATATG AAGTTGAAGG GTTTATTGAA 
AAGAATAGAG ACAGCGTTTC CCTAGGTCAT CTGGATGTTT TCAAAGCAAC GACAAATCCA 
ATTTTCAAAC AAATTTTAGA CAACAGAGAA CTTAGGAGTG ACGATGCTCC TGAAGAGCAA 
AATACTGAAA AAAAGATAAT GATCCCAGCA AGATTAAGTC AAAAGAAACC AACCCTGGGA 
TCTATGTTCA AAAAATCGCT CGGGGAATTG ATGGCCATCA TAAATTCTAC TAATGTTCAT 
TATATTCGTT GCATAAAACC AAATTCTGAA AAAAAGCCAT GGGAGTTCGA TAATTTGATG 
GTCTTATCGC AATTAAGAGC TTGTGGTGTG CTGGAAACAA TCAGGATTTC ATGTGCAGGC 
TTTCCATCAA GATGGACTTT TGACGAATTC GTTCAAAGAT ATTTCCTCCT GACGGACTAC 
AGCTTATGGA GTGGAATTCT TTATAATCCA GACCTCCCAA AAGAGGCAAT CGTCAACTTC 
TGTCAATCTA TTCTAGATGC TACTATCTCT GATTCAGCAA AATACCAAAT TGGTAATACC 
AAAATTTTCT TTAAAGCAGG AATGCTTGCA TTTTTGGAAA AGCTAAGGAC TAATAAAATG 
AATGAAATAT GCATCATAAT ACAGAAAAAG ATAAGGGCAA GATACTATAG GCTTCAGTAT 
TTGCAGACCA TGGAATCAAT AAAGAAGTGC CAAAGCCAAA TTAGAAGTTT GTTGGTTCGT 
ACAAGGGTCG ATCATGAGCT CAAGACAAGA GCTGCCATAT TACTACAAAC GAACATTAGA 
GCATTATGGA AACGGGAATA CTATAGAGCT GCCATTGGGC AAATAATAAA ACTACAATGT 
ACCTGTAAAA GAAAATTGAT TTTAGATAGT GTTAACAGGA AATTCATGCT CATGGCGGCA 
GTAATTATCC AAAGCTACAT CAGATCATAT GGTCACAAAA CAGATTACAG AACATTGAAA 
AGGAGCTCCA TTCTTGTACA AAGCGCCATG AGAATGCAGT TAGCTAGACG AAGGTATATA 
GTTCTGCAAA AAGAAGTTGA AGAACGGAAT ATTAGGGCTA GTTACGGGAT TGGATTACTG 
GAGGAAGCTA TCGAATTTAA GAACTCTTTC ATTTTGAATC TAGAGATGTT AAATGACTCA 
TATACAAGAT TAACACAGTT GTTGCAGGGA GATTTGTCAA ATATTCCATC TAAGCAAAGG 
CAAGAGTACG AAACGATAGT AAATGGTTAT AATGATAAGA TTTCCAAGCT GAAGACGTTA 
CAAGTTGAAA TCATGAATAC TTTAAACAAG AAGAATGCCT TGAAGGAGAG AAAAAAGAAG 
CAATCTTCTT TAATACAGTC ACATATGCAA AGTTTGGCTG CTATAAAAGG TAACAAACCT 
TCAAGATTAA GTGATGAAGT CAAATCAATG AAGCAAGAAC TAGCGTTTAT TGAAAACGTA 
ATAGCACAAG ATTTCACCAC CACATATTCT GCTAATAAGA ATGATAAAGT GAAAGGTTTG 
GGAATCGCAG GACAACAAGT AAAACCGAAG CTAGTCAATG TAATTCGTAG AGAATCAGGA 
AATCCTGATC TTCTAGAATT ACTAATGGAC CTGAATTGTT ACACATTAGA AGTCACAGAA 
GGTTACTTGA AAAAAGTAAA TGTTACTGAG GTGAATGGAG ACAATGTTCT GGGTCCTATA 
CACGTCATTA CTACAGTGGT AAGTAGCTTA GTGAGAAATG GGTTATTGAT TCAAAGTAGC 
AAATTCATCT CGAAAGTCCT ATTGACTGTA GAAAGCATTG TTATGAGTCT ACCAAAGGAC 
GAAACAATGC TTGGTGGTAT CTTTTGGTTA AGTAATCTTT CCAGACTTCC CGCATTTGCT 
GCAAATCAAA AAACATTATA TGAAGCGAAT GGTGGTGATG AAAAAGATAA GTTAACACTG 
ATATATTTGA ATGATTTGGA GAATGAAACT TTAAAAGTAT TCGACAAGAT TTATTCCACT 
TGGTTAGTGA AATTCATGAA GCATGCTTCA GCCCACATCG AGATCTTCGA TATGGTTTTA 
AACGAGAAGC TATTTAAAAA TTCTGGTGAT GAGAAGTTCG CTAAATTATT CACATTCTTA 
AATGAATTTG ATGCTGTCTT GTGTAAATTT CAAGTTGTTG ACTCAATGCA TACAAAAATT 
TTTAACGACA CCCTGAAGTA TCTGAACGTA ATGCTATTTA ATGACCTAAT AACCAAATGT 
CCCGCGTTAA ATTGGAAGTA TGGGTACGAA GTGGATAGAA ATATCGAGCG GTTGGTGAGC 
TGGTTTGAAC CAAGAATTGA AGACGTTCGT CCCAATTTGA TTCAAATAAT TCAGGCTGTC 
AAGATATTAC AGTTGAAGAT AAGCAACTTG AACGAGTTCA AGTTACTATT CGATTTCTGG 
TATGCCTTGA ATCCAGCCCA AATACAAGCA ATTCTGTTGA AATACAAGCC TGCTAACAAG 
GGCGAAGCTG GAGTACCGAA CGAAATCTTG AATTATTTAG CTAACGTGAT CAAAAGAGAA 
AATCTGTCTT TGCCAGGGAA GATGGAAATT ATGCTTTCTG CCCAATTTGA CTCTGCTAAA 
AACCATCTGC GTTATGACAC TAGTGCAATA ACACAGAACA GCAATACAGA GGGCTTAGCT 
ACTGTCAGTA AAATTATAAA ATTAGACAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB

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MYO4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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