CAK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CAK1
Entrez Gene ID	850515
Full Name	cyclin-dependent protein kinase-activating kinase CAK1
Synonyms	CIV1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179937.1	NP_116624.1	cyclin-dependent protein kinase-activating kinase CAK1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	CAK1	NP_116624.1
Kluyveromyces lactis	KLLA0F08877g	XP_455483.1
Eremothecium gossypii	AGOS_AFR150C	NP_985697.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

unusual stability of this Cak1:Cdc37 association may partly reflect unique structural features of the fungal Cak1
Title: Cdc37 engages in stable, S14A mutation-reinforced association with the most atypical member of the yeast kinome, Cdk-activating kinase (Cak1).

While Cak1 is constitutively active and expressed, dividing cells tightly regulate Cak1 protein levels to ensure presence of adequate levels of Cdc28 CDK activity.
Title: A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1.

Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Title: Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.

the interactions linking Cak1 with the PAF1 complex and with Ssu72 are not direct but mediated via Ctk1 and Bur1
Title: Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1.

Ctk1 phosphorylation on Thr-338 is carried out by Cak1 and is required for normal gene transcription during the transition into stationary phase
Title: Phosphorylation by Cak1 regulates the C-terminal domain kinase Ctk1 in Saccharomyces cerevisiae.

The following CAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00207	NM_001179937.1 Latest version!	Saccharomyces cerevisiae S288C cyclin-dependent protein kinase-activating kinase CAK1 (CAK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAK1 ( NM_001179937.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116624.1 Saccharomyces cerevisiae S288C cyclin-dependent protein kinase-activating kinase CAK1 (CAK1), partial mRNA.

Price & Availability↑

CloneID	OSi00207
Clone ID Related Accession (Same CDS sequence)	NM_001179937.1
Accession Version	NM_001179937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cyclin-dependent protein kinase-activating kinase CAK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAACTGG ATAGTATAGA CATTACACAC TGTCAGTTGG TCAAATCTAC TAGAACTGCT 
AGGATTTATA GGTCGGATAC ATATGCCATT AAATGTCTAG CACTAGATTT CGATATCCCG 
CCACATAACG CCAAATTCGA AGTATCGATA TTAAACAAAC TGGGCAACAA ATGTAAGCAC 
ATCTTACCTC TTCTAGAGTC TAAGGCTACC GATAATAATG ACCTATTGTT GTTGTTTCCC 
TTTGAAGAGA TGAACCTTTA TGAGTTCATG CAAATGCACT ATAAAAGAGA TAGAAGAAAA 
AAAAATCCCT ATTACGATTT GCTAAATCCC AGTATCCCAA TTGTTGCGGA CCCCCCCGTT 
CAGAAATATA CTAATCAATT GGACGTCAAT CGGTATTCTT TGTCCTTTTT CCGGCAAATG 
GTTGAAGGGA TTGCATTCTT ACATGAGAAC AAGATCATTC ACCGCGACAT CAAACCGCAA 
AATATCATGC TAACAAACAA TACCAGCACC GTATCCCCAA AGTTGTACAT AATTGATTTT 
GGCATCTCTT ATGACATGGC AAATAACTCA CAAACAAGTG CGGAACCCAT GGATAGCAAG 
GTGACGGATA TAAGCACAGG AATTTACAAG GCCCCAGAAG TGCTTTTTGG AGTAAAATGC 
TATGATGGTG GCGTGGACGT GTGGTCGTTG TTGATAATTA TTTCTCAGTG GTTCCAGAGA 
GAAACAAGCC GTATGGGGCA CGTTCCGGCC ATGATTGATG ACGGCAGCGA CGACATGAAC 
TCAGATGGAA GCGATTTCAG ACTGATTTGC TCAATATTCG AAAAGTTGGG CATACCGTCC 
ATTCAGAAAT GGGAAGAGGT TGCGCAACAC GGCTCGGTTG ATGCATTTGT TGGTATGTTT 
GGTGCAGATG GCGATGGCAA GTATGTACTG GACCAGGAGA AAGATGTACA GATTAGCATT 
GTTGAGAGGA ATATGCCTCG ACTGGACGAG ATTGCGGATG TCAAAGTCAA GCAGAAGTTC 
ATTAATTGTA TCCTGGGGAT GGTTTCATTT TCACCAAACG AAAGATGGAG CTGTCAAAGA 
ATCTTGCAAG AATTAGAAAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116624.1
CDS	1..1107
Translation	MKLDSIDITHCQLVKSTRTARIYRSDTYAIKCLALDFDIPPHNAKFEVSILNKLGNKCKHILPLLESKATDNNDLLLLFPFEEMNLYEFMQMHYKRDRRKKNPYYDLLNPSIPIVADPPVQKYTNQLDVNRYSLSFFRQMVEGIAFLHENKIIHRDIKPQNIMLTNNTSTVSPKLYIIDFGISYDMANNSQTSAEPMDSKVTDISTGIYKAPEVLFGVKCYDGGVDVWSLLIIISQWFQRETSRMGHVPAMIDDGSDDMNSDGSDFRLICSIFEKLGIPSIQKWEEVAQHGSVDAFVGMFGADGDGKYVLDQEKDVQISIVERNMPRLDEIADVKVKQKFINCILGMVSFSPNERWSCQRILQELEKP

Target ORF information:

RefSeq Version	NM_001179937.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cyclin-dependent protein kinase-activating kinase CAK1 (CAK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179937.1

ORF Insert Sequence:

ATGAAACTGG ATAGTATAGA CATTACACAC TGTCAGTTGG TCAAATCTAC TAGAACTGCT 
AGGATTTATA GGTCGGATAC ATATGCCATT AAATGTCTAG CACTAGATTT CGATATCCCG 
CCACATAACG CCAAATTCGA AGTATCGATA TTAAACAAAC TGGGCAACAA ATGTAAGCAC 
ATCTTACCTC TTCTAGAGTC TAAGGCTACC GATAATAATG ACCTATTGTT GTTGTTTCCC 
TTTGAAGAGA TGAACCTTTA TGAGTTCATG CAAATGCACT ATAAAAGAGA TAGAAGAAAA 
AAAAATCCCT ATTACGATTT GCTAAATCCC AGTATCCCAA TTGTTGCGGA CCCCCCCGTT 
CAGAAATATA CTAATCAATT GGACGTCAAT CGGTATTCTT TGTCCTTTTT CCGGCAAATG 
GTTGAAGGGA TTGCATTCTT ACATGAGAAC AAGATCATTC ACCGCGACAT CAAACCGCAA 
AATATCATGC TAACAAACAA TACCAGCACC GTATCCCCAA AGTTGTACAT AATTGATTTT 
GGCATCTCTT ATGACATGGC AAATAACTCA CAAACAAGTG CGGAACCCAT GGATAGCAAG 
GTGACGGATA TAAGCACAGG AATTTACAAG GCCCCAGAAG TGCTTTTTGG AGTAAAATGC 
TATGATGGTG GCGTGGACGT GTGGTCGTTG TTGATAATTA TTTCTCAGTG GTTCCAGAGA 
GAAACAAGCC GTATGGGGCA CGTTCCGGCC ATGATTGATG ACGGCAGCGA CGACATGAAC 
TCAGATGGAA GCGATTTCAG ACTGATTTGC TCAATATTCG AAAAGTTGGG CATACCGTCC 
ATTCAGAAAT GGGAAGAGGT TGCGCAACAC GGCTCGGTTG ATGCATTTGT TGGTATGTTT 
GGTGCAGATG GCGATGGCAA GTATGTACTG GACCAGGAGA AAGATGTACA GATTAGCATT 
GTTGAGAGGA ATATGCCTCG ACTGGACGAG ATTGCGGATG TCAAAGTCAA GCAGAAGTTC 
ATTAATTGTA TCCTGGGGAT GGTTTCATTT TCACCAAACG AAAGATGGAG CTGTCAAAGA 
ATCTTGCAAG AATTAGAAAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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CAK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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