DDI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DDI1
Entrez Gene ID	856886
Full Name	Ddi1p
Synonyms	VSM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179033.3	NP_011070.3	Ddi1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	DDI1	NP_011070.3
Eremothecium gossypii	AGOS_AFR010C	NP_985557.1
Neurospora crassa	NCU05292	XP_962063.2
Kluyveromyces lactis	KLLA0E10341g	XP_454416.1
Schizosaccharomyces pombe (fission yeast)	mud1	NP_001018195.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The dual functionality of Ddi1-ubiquitin-like domain, capable of binding both ubiquitin and proteasome, suggests an intriguing mechanism for Ddi1 as a proteasomal shuttle.
Title: DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin.

The current study identifies residues in the LRR1 domain of Rpn1 that contribute to shuttle receptor docking. It validates Ddi1 as a proteasomal shuttle receptor whose stable binding to the proteasome depends on Rpn1 residue D517.
Title: Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1.

Results substantiate the hypothesis that Ddi1 functions in vivo as a catalytically-active aspartic proteinase.
Title: The retroviral proteinase active site and the N-terminus of Ddi1 are required for repression of protein secretion.

Ddi1 participates in multiple cellular processes via its different domains and phosphorylation may regulate exocytic functions
Title: Different domains of the UBL-UBA ubiquitin receptor, Ddi1/Vsm1, are involved in its multiple cellular roles.

However, our results revealed that Ddi1p and its associated protein Rad23p are not involved in the decrease in Pho81p level under high-phosphate condition.
Title: Ddi1p and Rad23p play a cooperative role as negative regulators in the PHO pathway in Saccharomyces cerevisiae.

The following DDI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05638	NM_001179033.3 Latest version!	Saccharomyces cerevisiae S288C Ddi1p (DDI1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDI1 ( NM_001179033.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011070.3 Saccharomyces cerevisiae S288C Ddi1p (DDI1), partial mRNA.

Price & Availability↑

CloneID	OSi05638
Clone ID Related Accession (Same CDS sequence)	NM_001179033.3
Accession Version	NM_001179033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ddi1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179033.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTTAA CAATTTCAAA CGAACTTACT GGCGAGATAT ACGGCCCAAT TGAAGTCAGT 
GAAGATATGG CTTTAACTGA TCTAATAGCA TTACTGCAGG CCGATTGCGG GTTTGACAAA 
ACAAAACACG ACTTATATTA CAATATGGAC ATTCTAGACT CAAACAGAAC TCAGTCTTTA 
AAAGAACTAG GGCTCAAAAC CGATGACTTA TTATTGATTA GGGGTAAGAT TTCCAATTCC 
ATTCAAACAG ATGCTGCTAC TTTGTCCGAC GAAGCGTTCA TAGAGCAATT TAGACAAGAG 
CTACTTAATA ATCAAATGCT TAGATCACAA CTCATTTTGC AAATCCCTGG ATTGAATGAT 
TTAGTAAATG ACCCACTGTT GTTTAGGGAA AGATTGGGCC CGCTCATATT ACAAAGACGT 
TATGGTGGCT ATAACACCGC CATGAATCCT TTTGGCATCC CCCAAGATGA ATATACTAGA 
CTAATGGCCA ACCCCGATGA TCCTGACAAC AAGAAGAGGA TTGCAGAGCT TTTAGACCAA 
CAAGCTATCG ATGAACAGTT GCGCAATGCT ATCGAATATA CACCTGAAAT GTTTACCCAG 
GTCCCTATGC TTTACATTAA TATCGAAATA AATAACTACC CCGTAAAGGC ATTTGTAGAT 
ACAGGGGCTC AAACAACGAT TATGTCCACC AGGTTAGCGA AGAAAACTGG CCTGTCGAGA 
ATGATTGACA AGAGATTTAT TGGAGAAGCT CGTGGCGTAG GAACCGGCAA AATTATTGGG 
AGAATTCACC AAGCCCAAGT CAAAATAGAA ACACAATATA TTCCATGCAG TTTTACCGTC 
TTAGATACTG ACATCGATGT TTTGATAGGA CTAGACATGC TGAAAAGGCA TTTGGCTTGT 
GTGGACTTAA AGGAAAACGT TCTTAGGATA GCAGAAGTGG AGACAAGCTT TTTGAGTGAA 
GCGGAAATCC CAAAAAGTTT TCAAGAAGGA CTTCCAGCAC CAACGTCAGT TACAACTTCG 
TCTGATAAGC CCCTAACACC CACCAAGACT AGTTCAACTC TACCACCACA ACCGGGGGCT 
GTTCCTGCGC TTGCTCCAAG GACTGGTATG GGGCCAACAC CTACGGGAAG AAGTACAGCC 
GGTGCTACAA CGGCAACCGG GAGAACGTTT CCCGAACAAA CGATTAAACA GCTTATGGAT 
CTAGGTTTCC CTAGGGATGC TGTGGTAAAG GCTCTTAAAC AGACTAACGG AAATGCAGAA 
TTTGCTGCAT CCCTCCTTTT CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011070.3
CDS	1..1287
Translation	MDLTISNELTGEIYGPIEVSEDMALTDLIALLQADCGFDKTKHDLYYNMDILDSNRTQSLKELGLKTDDLLLIRGKISNSIQTDAATLSDEAFIEQFRQELLNNQMLRSQLILQIPGLNDLVNDPLLFRERLGPLILQRRYGGYNTAMNPFGIPQDEYTRLMANPDDPDNKKRIAELLDQQAIDEQLRNAIEYTPEMFTQVPMLYINIEINNYPVKAFVDTGAQTTIMSTRLAKKTGLSRMIDKRFIGEARGVGTGKIIGRIHQAQVKIETQYIPCSFTVLDTDIDVLIGLDMLKRHLACVDLKENVLRIAEVETSFLSEAEIPKSFQEGLPAPTSVTTSSDKPLTPTKTSSTLPPQPGAVPALAPRTGMGPTPTGRSTAGATTATGRTFPEQTIKQLMDLGFPRDAVVKALKQTNGNAEFAASLLFQ

Target ORF information:

RefSeq Version	NM_001179033.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ddi1p (DDI1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179033.3

ORF Insert Sequence:

ATGGATTTAA CAATTTCAAA CGAACTTACT GGCGAGATAT ACGGCCCAAT TGAAGTCAGT 
GAAGATATGG CTTTAACTGA TCTAATAGCA TTACTGCAGG CCGATTGCGG GTTTGACAAA 
ACAAAACACG ACTTATATTA CAATATGGAC ATTCTAGACT CAAACAGAAC TCAGTCTTTA 
AAAGAACTAG GGCTCAAAAC CGATGACTTA TTATTGATTA GGGGTAAGAT TTCCAATTCC 
ATTCAAACAG ATGCTGCTAC TTTGTCCGAC GAAGCGTTCA TAGAGCAATT TAGACAAGAG 
CTACTTAATA ATCAAATGCT TAGATCACAA CTCATTTTGC AAATCCCTGG ATTGAATGAT 
TTAGTAAATG ACCCACTGTT GTTTAGGGAA AGATTGGGCC CGCTCATATT ACAAAGACGT 
TATGGTGGCT ATAACACCGC CATGAATCCT TTTGGCATCC CCCAAGATGA ATATACTAGA 
CTAATGGCCA ACCCCGATGA TCCTGACAAC AAGAAGAGGA TTGCAGAGCT TTTAGACCAA 
CAAGCTATCG ATGAACAGTT GCGCAATGCT ATCGAATATA CACCTGAAAT GTTTACCCAG 
GTCCCTATGC TTTACATTAA TATCGAAATA AATAACTACC CCGTAAAGGC ATTTGTAGAT 
ACAGGGGCTC AAACAACGAT TATGTCCACC AGGTTAGCGA AGAAAACTGG CCTGTCGAGA 
ATGATTGACA AGAGATTTAT TGGAGAAGCT CGTGGCGTAG GAACCGGCAA AATTATTGGG 
AGAATTCACC AAGCCCAAGT CAAAATAGAA ACACAATATA TTCCATGCAG TTTTACCGTC 
TTAGATACTG ACATCGATGT TTTGATAGGA CTAGACATGC TGAAAAGGCA TTTGGCTTGT 
GTGGACTTAA AGGAAAACGT TCTTAGGATA GCAGAAGTGG AGACAAGCTT TTTGAGTGAA 
GCGGAAATCC CAAAAAGTTT TCAAGAAGGA CTTCCAGCAC CAACGTCAGT TACAACTTCG 
TCTGATAAGC CCCTAACACC CACCAAGACT AGTTCAACTC TACCACCACA ACCGGGGGCT 
GTTCCTGCGC TTGCTCCAAG GACTGGTATG GGGCCAACAC CTACGGGAAG AAGTACAGCC 
GGTGCTACAA CGGCAACCGG GAGAACGTTT CCCGAACAAA CGATTAAACA GCTTATGGAT 
CTAGGTTTCC CTAGGGATGC TGTGGTAAAG GCTCTTAAAC AGACTAACGG AAATGCAGAA 
TTTGCTGCAT CCCTCCTTTT CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

DDI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報