YME1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YME1
Entrez Gene ID	856135
Full Name	i-AAA protease YME1
Synonyms	OSD1,YTA11
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184121.1	NP_015349.1	i-AAA protease YME1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YME1	NP_015349.1
Eremothecium gossypii	AGOS_AGL274W	NP_986393.1
Oryza sativa (rice)	Os01g0574500	NP_001043385.1
Kluyveromyces lactis	KLLA0E06711g	XP_454250.1
Arabidopsis thaliana (thale cress)	ftsh4	NP_565616.1
Magnaporthe oryzae (rice blast fungus)	MGG_03926	XP_003719938.1
Oryza sativa (rice)	Os01g0574400	NP_001043384.1
Schizosaccharomyces pombe (fission yeast)	SPCC965.04c	NP_588514.1
Neurospora crassa	NCU03359	XP_956468.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify an important role for the mitochondrial protease Yme1 in the ability of cells that lack tafazzin function to maintain mitochondrial structural integrity and mitochondrial quality control and to undergo mitophagy
Title: The mitochondrial quality control protein Yme1 is necessary to prevent defective mitophagy in a yeast model of Barth syndrome.

Yme1 role in Tim10 degradation
Title: Mitochondrial Tim9 protects Tim10 from degradation by the protease Yme1.

No individual cysteine residue is required for the function of Tim9 or Tim10 in yeast and defective assembly of the small TIMs induces their proteolytic clearance from mitochondria via the mitochondrial ATP-dependent protease, Yme1.
Title: Impaired folding of the mitochondrial small TIM chaperones induces clearance by the i-AAA protease.

Inactivation of either Yme1 or Yta10/12, allows fermentative growth of hsc82 or hsp82 strains at 37 degrees C.
Title: Hsp90 and mitochondrial proteases Yme1 and Yta10/12 participate in ATP synthase assembly in Saccharomyces cerevisiae.

Yme1 probably chaperones the folding and/or assembly of Oxa1-exported Cox2 in the absence of Mrg1 or Mgr3, since respiratory growth and cytochrome c oxidase assembly in a cox18 mgr3 double-mutant strain overexpressing OXA1 is YME1 dependent
Title: Translocation and assembly of mitochondrially coded Saccharomyces cerevisiae cytochrome c oxidase subunit Cox2 by Oxa1 and Yme1 in the absence of Cox18.

The following YME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04985	NM_001184121.1 Latest version!	Saccharomyces cerevisiae S288C i-AAA protease YME1 (YME1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YME1 ( NM_001184121.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015349.1 Saccharomyces cerevisiae S288C i-AAA protease YME1 (YME1), partial mRNA.

Price & Availability↑

CloneID	OSi04985
Clone ID Related Accession (Same CDS sequence)	NM_001184121.1
Accession Version	NM_001184121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	i-AAA protease YME1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACGTTT CAAAAATACT TGTGTCGCCC ACGGTCACGA CAAATGTTTT ACGCATATTT 
GCTCCCAGGC TACCTCAAAT CGGTGCTTCT TTGTTAGTTC AAAAAAAATG GGCCTTAAGA 
TCAAAGAAGT TCTACCGTTT TTATTCTGAA AAGAATAGCG GTGAAATGCC TCCTAAGAAG 
GAAGCTGATA GCTCTGGAAA GGCATCCAAT AAATCCACGA TATCTTCAAT CGACAATTCG 
CAACCACCAC CTCCATCGAA CACTAATGAT AAAACCAAAC AAGCGAACGT AGCTGTGTCA 
CATGCTATGC TAGCAACTAG AGAACAAGAG GCCAATAAAG ACTTAACGAG TCCTGATGCA 
CAAGCAGCCT TTTACAAACT TCTCTTACAA TCAAACTACC CGCAATACGT GGTCTCTAGG 
TTTGAGACCC CCGGTATTGC GTCATCGCCT GAATGCATGG AACTGTACAT GGAGGCCCTG 
CAGAGGATAG GCAGACACTC GGAGGCTGAT GCCGTTAGAC AAAACCTACT GACAGCCAGC 
TCTGCTGGCG CAGTTAACCC ATCATTGGCG TCATCTTCAT CAAACCAGTC AGGTTATCAT 
GGTAACTTTC CATCGATGTA TTCTCCGCTT TATGGATCTC GCAAAGAGCC ACTACATGTT 
GTCGTATCTG AATCAACTTT TACTGTTGTA TCGAGATGGG TAAAGTGGCT GCTTGTTTTC 
GGTATCTTAA CCTACTCTTT TTCTGAAGGT TTTAAATACA TCACAGAAAA TACAACGCTA 
CTAAAGTCGT CAGAAGTAGC CGACAAATCA GTTGATGTAG CTAAGACAAA TGTTAAATTT 
GATGATGTCT GCGGTTGTGA TGAGGCCCGT GCGGAATTGG AAGAAATTGT TGATTTCCTG 
AAAGATCCAA CTAAGTACGA GTCCTTGGGT GGTAAACTAC CAAAGGGTGT ACTGTTGACT 
GGACCTCCTG GTACAGGTAA AACTTTGTTG GCTAGGGCCA CTGCCGGAGA GGCTGGTGTA 
GATTTTTTCT TTATGTCAGG TTCTGAATTT GATGAAGTTT ACGTCGGTGT TGGTGCTAAA 
CGTATCCGTG ATTTGTTTGC TCAAGCGCGT TCTCGTGCAC CAGCTATTAT TTTTATTGAT 
GAATTAGATG CCATCGGTGG TAAGCGTAAT CCAAAGGACC AAGCTTACGC CAAACAAACG 
TTGAATCAGT TATTGGTCGA ATTAGATGGT TTCTCACAAA CAAGTGGAAT TATTATTATT 
GGTGCCACAA ATTTCCCTGA GGCTTTAGAT AAGGCGTTAA CTAGACCAGG TAGATTTGAT 
AAGGTTGTGA ATGTGGATTT GCCAGATGTT CGTGGTCGTG CTGATATTTT AAAGCATCAC 
ATGAAGAAGA TTACATTGGC AGACAATGTG GATCCAACTA TTATTGCGCG TGGTACCCCC 
GGTTTATCAG GCGCTGAGCT GGCAAATTTA GTCAACCAAG CAGCAGTTTA CGCGTGTCAA 
AAAAATGCTG TTTCCGTTGA TATGTCCCAC TTCGAGTGGG CTAAGGATAA GATATTGATG 
GGTGCTGAGA GAAAGACTAT GGTCTTAACA GATGCAGCCA GAAAGGCCAC TGCTTTCCAC 
GAGGCTGGAC ATGCCATTAT GGCCAAATAC ACCAATGGTG CTACCCCGCT ATACAAGGCC 
ACGATATTGC CTAGAGGTAG GGCATTGGGT ATTACTTTTC AATTGCCAGA AATGGATAAG 
GTCGACATCA CCAAAAGGGA GTGTCAAGCC AGACTGGACG TGTGCATGGG GGGCAAAATT 
GCAGAAGAAT TAATTTATGG TAAAGATAAC ACCACAAGTG GTTGTGGGTC TGACTTGCAA 
AGCGCCACCG GCACAGCAAG GGCTATGGTT ACTCAATATG GTATGAGTGA TGATGTAGGT 
CCCGTTAACT TGTCAGAAAA TTGGGAATCT TGGTCTAATA AGATTCGCGA TATTGCTGAT 
AATGAAGTGA TTGAACTTTT GAAGGACTCC GAGGAAAGAG CAAGAAGACT ATTAACTAAG 
AAAAATGTTG AGCTACATAG ACTTGCGCAA GGTCTTATTG AATATGAAAC TCTAGATGCC 
CACGAAATCG AACAAGTTTG TAAAGGTGAA AAACTGGACA AACTGAAAAC TTCCACCAAT 
ACAGTCGTAG AAGGACCAGA CAGTGATGAA CGTAAAGATA TAGGCGATGA TAAACCCAAA 
ATTCCTACAA TGTTAAATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015349.1
CDS	1..2244
Translation	MNVSKILVSPTVTTNVLRIFAPRLPQIGASLLVQKKWALRSKKFYRFYSEKNSGEMPPKKEADSSGKASNKSTISSIDNSQPPPPSNTNDKTKQANVAVSHAMLATREQEANKDLTSPDAQAAFYKLLLQSNYPQYVVSRFETPGIASSPECMELYMEALQRIGRHSEADAVRQNLLTASSAGAVNPSLASSSSNQSGYHGNFPSMYSPLYGSRKEPLHVVVSESTFTVVSRWVKWLLVFGILTYSFSEGFKYITENTTLLKSSEVADKSVDVAKTNVKFDDVCGCDEARAELEEIVDFLKDPTKYESLGGKLPKGVLLTGPPGTGKTLLARATAGEAGVDFFFMSGSEFDEVYVGVGAKRIRDLFAQARSRAPAIIFIDELDAIGGKRNPKDQAYAKQTLNQLLVELDGFSQTSGIIIIGATNFPEALDKALTRPGRFDKVVNVDLPDVRGRADILKHHMKKITLADNVDPTIIARGTPGLSGAELANLVNQAAVYACQKNAVSVDMSHFEWAKDKILMGAERKTMVLTDAARKATAFHEAGHAIMAKYTNGATPLYKATILPRGRALGITFQLPEMDKVDITKRECQARLDVCMGGKIAEELIYGKDNTTSGCGSDLQSATGTARAMVTQYGMSDDVGPVNLSENWESWSNKIRDIADNEVIELLKDSEERARRLLTKKNVELHRLAQGLIEYETLDAHEIEQVCKGEKLDKLKTSTNTVVEGPDSDERKDIGDDKPKIPTMLNA

Target ORF information:

RefSeq Version	NM_001184121.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C i-AAA protease YME1 (YME1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184121.1

ORF Insert Sequence:

ATGAACGTTT CAAAAATACT TGTGTCGCCC ACGGTCACGA CAAATGTTTT ACGCATATTT 
GCTCCCAGGC TACCTCAAAT CGGTGCTTCT TTGTTAGTTC AAAAAAAATG GGCCTTAAGA 
TCAAAGAAGT TCTACCGTTT TTATTCTGAA AAGAATAGCG GTGAAATGCC TCCTAAGAAG 
GAAGCTGATA GCTCTGGAAA GGCATCCAAT AAATCCACGA TATCTTCAAT CGACAATTCG 
CAACCACCAC CTCCATCGAA CACTAATGAT AAAACCAAAC AAGCGAACGT AGCTGTGTCA 
CATGCTATGC TAGCAACTAG AGAACAAGAG GCCAATAAAG ACTTAACGAG TCCTGATGCA 
CAAGCAGCCT TTTACAAACT TCTCTTACAA TCAAACTACC CGCAATACGT GGTCTCTAGG 
TTTGAGACCC CCGGTATTGC GTCATCGCCT GAATGCATGG AACTGTACAT GGAGGCCCTG 
CAGAGGATAG GCAGACACTC GGAGGCTGAT GCCGTTAGAC AAAACCTACT GACAGCCAGC 
TCTGCTGGCG CAGTTAACCC ATCATTGGCG TCATCTTCAT CAAACCAGTC AGGTTATCAT 
GGTAACTTTC CATCGATGTA TTCTCCGCTT TATGGATCTC GCAAAGAGCC ACTACATGTT 
GTCGTATCTG AATCAACTTT TACTGTTGTA TCGAGATGGG TAAAGTGGCT GCTTGTTTTC 
GGTATCTTAA CCTACTCTTT TTCTGAAGGT TTTAAATACA TCACAGAAAA TACAACGCTA 
CTAAAGTCGT CAGAAGTAGC CGACAAATCA GTTGATGTAG CTAAGACAAA TGTTAAATTT 
GATGATGTCT GCGGTTGTGA TGAGGCCCGT GCGGAATTGG AAGAAATTGT TGATTTCCTG 
AAAGATCCAA CTAAGTACGA GTCCTTGGGT GGTAAACTAC CAAAGGGTGT ACTGTTGACT 
GGACCTCCTG GTACAGGTAA AACTTTGTTG GCTAGGGCCA CTGCCGGAGA GGCTGGTGTA 
GATTTTTTCT TTATGTCAGG TTCTGAATTT GATGAAGTTT ACGTCGGTGT TGGTGCTAAA 
CGTATCCGTG ATTTGTTTGC TCAAGCGCGT TCTCGTGCAC CAGCTATTAT TTTTATTGAT 
GAATTAGATG CCATCGGTGG TAAGCGTAAT CCAAAGGACC AAGCTTACGC CAAACAAACG 
TTGAATCAGT TATTGGTCGA ATTAGATGGT TTCTCACAAA CAAGTGGAAT TATTATTATT 
GGTGCCACAA ATTTCCCTGA GGCTTTAGAT AAGGCGTTAA CTAGACCAGG TAGATTTGAT 
AAGGTTGTGA ATGTGGATTT GCCAGATGTT CGTGGTCGTG CTGATATTTT AAAGCATCAC 
ATGAAGAAGA TTACATTGGC AGACAATGTG GATCCAACTA TTATTGCGCG TGGTACCCCC 
GGTTTATCAG GCGCTGAGCT GGCAAATTTA GTCAACCAAG CAGCAGTTTA CGCGTGTCAA 
AAAAATGCTG TTTCCGTTGA TATGTCCCAC TTCGAGTGGG CTAAGGATAA GATATTGATG 
GGTGCTGAGA GAAAGACTAT GGTCTTAACA GATGCAGCCA GAAAGGCCAC TGCTTTCCAC 
GAGGCTGGAC ATGCCATTAT GGCCAAATAC ACCAATGGTG CTACCCCGCT ATACAAGGCC 
ACGATATTGC CTAGAGGTAG GGCATTGGGT ATTACTTTTC AATTGCCAGA AATGGATAAG 
GTCGACATCA CCAAAAGGGA GTGTCAAGCC AGACTGGACG TGTGCATGGG GGGCAAAATT 
GCAGAAGAAT TAATTTATGG TAAAGATAAC ACCACAAGTG GTTGTGGGTC TGACTTGCAA 
AGCGCCACCG GCACAGCAAG GGCTATGGTT ACTCAATATG GTATGAGTGA TGATGTAGGT 
CCCGTTAACT TGTCAGAAAA TTGGGAATCT TGGTCTAATA AGATTCGCGA TATTGCTGAT 
AATGAAGTGA TTGAACTTTT GAAGGACTCC GAGGAAAGAG CAAGAAGACT ATTAACTAAG 
AAAAATGTTG AGCTACATAG ACTTGCGCAA GGTCTTATTG AATATGAAAC TCTAGATGCC 
CACGAAATCG AACAAGTTTG TAAAGGTGAA AAACTGGACA AACTGAAAAC TTCCACCAAT 
ACAGTCGTAG AAGGACCAGA CAGTGATGAA CGTAAAGATA TAGGCGATGA TAAACCCAAA 
ATTCCTACAA TGTTAAATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YME1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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