FAS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FAS2
Entrez Gene ID	855845
Full Name	trifunctional fatty acid synthase subunit FAS2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184045.1	NP_015093.1	trifunctional fatty acid synthase subunit FAS2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FAS2	NP_015093.1
Kluyveromyces lactis	KLLA0C15983g	XP_452914.1
Neurospora crassa	NCU07308	XP_962466.1
Eremothecium gossypii	AGOS_AFL138W	NP_985412.2
Schizosaccharomyces pombe (fission yeast)	fas2	NP_593823.1
Magnaporthe oryzae (rice blast fungus)	MGG_12154	XP_003719713.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FAS2 molecular structure shows how acyl chain interacts with the hydrophobic cavity and gives it stability.
Title: Interactions of the acyl chain with the Saccharomyces cerevisiae acyl carrier protein.

The major ubiquitin ligase targeting the superfluous Fas2 subunit to the proteasome is Ubr1. The ubiquitin-conjugating enzymes Ubc2 and Ubc4 assist the degradation process.
Title: Quality control of a cytoplasmic protein complex: chaperone motors and the ubiquitin-proteasome system govern the fate of orphan fatty acid synthase subunit Fas2 of yeast.

Overexpression of FAS1, FAS2 and Acc1 leads to accumulation of sterols.
Title: Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae.

The following FAS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04726	NM_001184045.1 Latest version!	Saccharomyces cerevisiae S288C trifunctional fatty acid synthase subunit FAS2 (FAS2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAS2 ( NM_001184045.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015093.1 Saccharomyces cerevisiae S288C trifunctional fatty acid synthase subunit FAS2 (FAS2), partial mRNA.

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CloneID	OSi04726
Clone ID Related Accession (Same CDS sequence)	NM_001184045.1
Accession Version	NM_001184045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	trifunctional fatty acid synthase subunit FAS2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGCCGG AAGTTGAGCA AGAATTAGCT CATATTTTGC TAACTGAATT GTTAGCTTAT 
CAATTTGCCT CTCCTGTGAG ATGGATTGAA ACTCAAGATG TTTTTTTGAA GGATTTTAAC 
ACTGAAAGGG TTGTTGAAAT CGGTCCTTCT CCAACTTTGG CTGGGATGGC TCAAAGAACC 
TTGAAGAATA AATACGAATC TTACGATGCT GCTCTGTCTT TACATAGAGA AATCTTATGC 
TATTCGAAGG ATGCCAAAGA GATTTATTAT ACCCCAGATC CATCCGAACT AGCTGCAAAG 
GAAGAGCCCG CTAAGGAAGA AGCTCCTGCT CCAACTCCAG CTGCTAGTGC TCCTGCTCCT 
GCAGCAGCAG CCCCAGCTCC CGTCGCGGCA GCAGCCCCAG CTGCAGCAGC TGCTGAGATT 
GCCGATGAAC CTGTCAAGGC TTCCCTATTG TTGCACGTTT TGGTTGCTCA CAAGTTGAAG 
AAGTCGTTAG ATTCCATTCC AATGTCCAAG ACAATCAAAG ACTTGGTCGG TGGTAAATCT 
ACAGTCCAAA ATGAAATTTT GGGTGATTTA GGTAAAGAAT TTGGTACTAC TCCTGAAAAA 
CCAGAAGAAA CTCCATTAGA AGAATTGGCA GAAACTTTCC AAGATACCTT CTCTGGAGCA 
TTGGGTAAGC AATCTTCCTC GTTATTATCA AGATTAATCT CATCTAAGAT GCCTGGTGGG 
TTTACTATTA CTGTCGCTAG AAAATACTTA CAAACTCGCT GGGGACTACC ATCTGGTAGA 
CAAGATGGTG TCCTTTTGGT AGCTTTATCT AACGAGCCTG CTGCTCGTCT AGGTTCTGAA 
GCTGATGCCA AGGCTTTCTT GGACTCCATG GCTCAAAAAT ACGCTTCCAT TGTTGGTGTT 
GACTTATCAT CAGCTGCTAG CGCTAGTGGT GCTGCCGGTG CAGGTGCTGC TGCCGGTGCA 
GCTATGATCG ATGCTGGCGC TCTGGAAGAA ATAACCAAAG ACCACAAGGT TTTGGCGCGT 
CAACAACTGC AAGTATTGGC TCGTTATCTA AAAATGGACT TGGATAACGG TGAAAGAAAG 
TTCTTGAAAG AAAAGGACAC TGTTGCTGAA CTTCAAGCTC AGTTGGATTA CTTGAATGCC 
GAATTAGGTG AATTCTTTGT TAACGGTGTT GCTACTTCTT TCTCTAGAAA AAAGGCCAGA 
ACCTTCGATT CTTCCTGGAA CTGGGCTAAA CAATCTTTAT TATCATTATA CTTTGAGATA 
ATTCATGGTG TCTTGAAAAA CGTTGATAGA GAGGTTGTTA GTGAAGCTAT CAATATCATG 
AACAGATCTA ACGATGCTTT GATTAAATTC ATGGAATACC ATATCTCTAA CACTGATGAA 
ACAAAAGGTG AAAACTATCA ATTGGTTAAA ACTCTTGGTG AGCAGTTGAT TGAAAACTGT 
AAACAAGTTT TGGATGTTGA TCCAGTTTAC AAAGATGTTG CTAAGCCTAC CGGTCCAAAA 
ACTGCTATTG ACAAGAACGG TAACATTACA TACTCAGAAG AGCCAAGAGA AAAGGTTAGG 
AAATTATCTC AATACGTACA AGAAATGGCC CTTGGTGGTC CAATCACCAA AGAATCTCAA 
CCTACTATTG AAGAGGATTT GACTCGTGTT TACAAGGCAA TCAGTGCTCA AGCTGATAAA 
CAAGATATTT CCAGCTCCAC CAGGGTTGAA TTTGAAAAAC TATATAGTGA TTTGATGAAG 
TTCTTGGAAA GCTCCAAAGA AATCGATCCT TCTCAAACAA CCCAATTGGC CGGTATGGAT 
GTTGAGGATG CTTTGGACAA AGATTCCACC AAAGAAGTTG CTTCTTTGCC AAACAAATCT 
ACCATTTCTA AGACGGTATC TTCAACTATT CCAAGAGAAA CTATTCCGTT CTTACATTTG 
AGAAAGAAGA CTCCTGCCGG AGATTGGAAA TATGACCGCC AATTGTCTTC TCTTTTCTTA 
GATGGTTTAG AAAAGGCTGC CTTCAACGGT GTCACCTTCA AGGACAAATA CGTCTTGATC 
ACTGGTGCTG GTAAGGGTTC TATTGGTGCT GAAGTCTTGC AAGGTTTGTT ACAAGGTGGT 
GCTAAGGTTG TTGTTACCAC CTCTCGTTTC TCTAAGCAAG TTACAGACTA CTACCAATCC 
ATTTACGCCA AATATGGTGC TAAGGGTTCT ACTTTGATTG TTGTTCCATT CAACCAAGGT 
TCTAAGCAAG ACGTTGAAGC TTTGATTGAA TTTATCTACG ACACTGAAAA GAATGGTGGT 
TTAGGTTGGG ATCTAGATGC TATTATTCCA TTCGCGGCCA TTCCAGAACA AGGTATTGAA 
TTAGAACATA TTGATTCTAA GTCTGAATTT GCTCATAGAA TCATGTTGAC CAATATCTTA 
AGAATGATGG GTTGTGTCAA GAAGCAAAAA TCTGCAAGAG GTATTGAAAC AAGACCAGCT 
CAAGTCATTC TACCAATGTC TCCAAACCAT GGTACTTTCG GTGGTGATGG TATGTATTCA 
GAATCCAAGT TGTCTTTGGA AACTTTGTTC AACAGATGGC ACTCTGAATC CTGGGCCAAT 
CAATTAACCG TTTGCGGTGC TATTATTGGT TGGACTAGAG GTACTGGTTT AATGAGCGCT 
AATAACATCA TTGCTGAAGG CATTGAAAAG ATGGGTGTTC GTACTTTCTC TCAAAAGGAA 
ATGGCTTTCA ACTTATTGGG TCTATTGACT CCAGAAGTCG TAGAATTGTG CCAAAAATCA 
CCTGTTATGG CTGACTTGAA TGGTGGTTTG CAATTTGTTC CTGAATTGAA GGAATTCACT 
GCTAAATTGC GTAAAGAGTT GGTTGAAACT TCTGAAGTTA GAAAGGCAGT TTCCATCGAA 
ACTGCTTTGG AGCATAAGGT TGTCAATGGC AATAGCGCTG ATGCTGCATA TGCTCAAGTC 
GAAATTCAAC CAAGAGCTAA CATTCAACTG GACTTCCCAG AATTGAAACC ATACAAACAG 
GTTAAACAAA TTGCTCCCGC TGAGCTTGAA GGTTTGTTGG ATTTGGAAAG AGTTATTGTA 
GTTACCGGTT TTGCTGAAGT CGGCCCATGG GGTTCGGCCA GAACAAGATG GGAAATGGAA 
GCTTTTGGTG AATTTTCGTT GGAAGGTTGC GTTGAAATGG CCTGGATTAT GGGCTTCATT 
TCATACCATA ACGGTAATTT GAAGGGTCGT CCATACACTG GTTGGGTTGA TTCCAAAACA 
AAAGAACCAG TTGATGACAA GGACGTTAAG GCCAAGTATG AAACATCAAT CCTAGAACAC 
AGTGGTATCA GATTGATCGA ACCAGAGTTA TTCAATGGTT ACAACCCAGA AAAGAAGGAA 
ATGATTCAAG AAGTCATTGT CGAAGAAGAC TTGGAACCAT TTGAGGCTTC GAAGGAAACT 
GCCGAACAAT TTAAACACCA ACATGGTGAC AAAGTGGATA TCTTCGAAAT CCCAGAAACA 
GGAGAGTACT CTGTTAAGTT ACTAAAGGGT GCCACTTTAT ACATTCCAAA GGCTTTGAGA 
TTTGACCGTT TGGTTGCAGG TCAAATTCCA ACTGGTTGGA ATGCTAAGAC TTATGGTATC 
TCTGATGATA TCATTTCTCA GGTTGACCCA ATCACATTAT TCGTTTTGGT CTCTGTTGTG 
GAAGCATTTA TTGCATCTGG TATCACCGAC CCATACGAAA TGTACAAATA CGTACATGTT 
TCTGAGGTTG GTAACTGTTC TGGTTCTGGT ATGGGTGGTG TTTCTGCCTT ACGTGGTATG 
TTTAAGGACC GTTTCAAGGA TGAGCCTGTC CAAAATGATA TTTTACAAGA ATCATTTATC 
AACACCATGT CCGCTTGGGT TAATATGTTG TTGATTTCCT CATCTGGTCC AATCAAGACA 
CCTGTTGGTG CCTGTGCCAC ATCCGTGGAA TCTGTTGACA TTGGTGTAGA AACCATCTTG 
TCTGGTAAGG CTAGAATCTG TATTGTCGGT GGTTACGATG ATTTCCAAGA AGAAGGCTCC 
TTTGAGTTCG GTAACATGAA GGCCACTTCC AACACTTTGG AAGAATTTGA ACATGGTCGT 
ACCCCAGCGG AAATGTCCAG ACCTGCCACC ACTACCCGTA ACGGTTTTAT GGAAGCTCAA 
GGTGCTGGTA TTCAAATCAT CATGCAAGCT GATTTAGCTT TGAAGATGGG TGTGCCAATT 
TACGGTATTG TTGCCATGGC TGCTACCGCC ACCGATAAGA TTGGTAGATC TGTGCCAGCT 
CCAGGTAAGG GTATTTTAAC CACTGCTCGT GAACACCACT CCAGTGTTAA GTATGCTTCA 
CCAAACTTGA ACATGAAGTA CAGAAAGCGC CAATTGGTTA CTCGTGAAGC TCAGATTAAA 
GATTGGGTAG AAAACGAATT GGAAGCTTTG AAGTTGGAGG CCGAAGAAAT TCCAAGCGAA 
GACCAAAACG AGTTCTTACT TGAACGTACC AGAGAAATCC ACAACGAAGC TGAAAGTCAA 
TTGAGAGCTG CACAACAACA ATGGGGTAAC GACTTCTACA AGAGGGACCC ACGTATTGCT 
CCATTGAGAG GAGCACTGGC TACTTACGGT TTAACTATTG ATGACTTGGG TGTCGCTTCA 
TTCCACGGTA CATCCACAAA GGCTAATGAC AAGAACGAAT CTGCCACAAT TAATGAAATG 
ATGAAGCATT TGGGTAGATC TGAAGGTAAT CCCGTCATTG GTGTTTTCCA AAAGTTCTTG 
ACTGGTCATC CAAAGGGTGC TGCTGGTGCA TGGATGATGA ATGGTGCTTT GCAAATTCTA 
AACAGTGGTA TTATTCCAGG TAACCGTAAC GCTGATAACG TGGATAAGAT CTTGGAGCAA 
TTTGAATACG TCTTGTACCC ATCCAAGACT TTAAAGACCG ACGGTGTCAG AGCCGTGTCC 
ATCACTTCTT TCGGTTTTGG TCAAAAGGGT GGTCAAGCTA TTGTGGTTCA TCCAGACTAC 
TTATACGGTG CTATCACTGA AGACAGATAC AACGAGTATG TCGCCAAGGT TAGTGCCAGA 
GAGAAAAGTG CCTACAAATT CTTCCATAAT GGTATGATCT ACAACAAGTT GTTCGTAAGT 
AAAGAGCATG CTCCATACAC TGATGAATTG GAAGAGGATG TTTACTTGGA CCCATTAGCC 
CGTGTATCTA AGGATAAGAA ATCAGGCTCC TTGACTTTCA ACTCTAAAAA CATCCAAAGC 
AAGGACAGTT ACATCAATGC TAACACCATT GAAACTGCCA AGATGATTGA AAACATGACC 
AAGGAGAAAG TCTCTAACGG TGGCGTCGGT GTAGATGTTG AATTAATCAC TAGCATCAAC 
GTTGAAAATG ATACTTTTAT CGAGCGCAAT TTCACCCCGC AAGAAATAGA GTACTGCAGC 
GCGCAGCCTA GTGTGCAAAG CTCTTTCGCT GGGACATGGT CCGCCAAAGA GGCTGTTTTC 
AAGTCCTTAG GCGTCAAGTC CTTAGGCGGT GGTGCTGCAT TGAAAGACAT CGAAATCGTA 
CGCGTTAACA AAAACGCTCC AGCCGTTGAA CTGCACGGTA ACGCCAAAAA GGCTGCCGAA 
GAAGCTGGTG TTACCGATGT GAAGGTATCT ATTTCTCACG ATGACCTCCA AGCTGTCGCG 
GTCGCCGTTT CTACTAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015093.1
CDS	1..5664
Translation	MKPEVEQELAHILLTELLAYQFASPVRWIETQDVFLKDFNTERVVEIGPSPTLAGMAQRTLKNKYESYDAALSLHREILCYSKDAKEIYYTPDPSELAAKEEPAKEEAPAPTPAASAPAPAAAAPAPVAAAAPAAAAAEIADEPVKASLLLHVLVAHKLKKSLDSIPMSKTIKDLVGGKSTVQNEILGDLGKEFGTTPEKPEETPLEELAETFQDTFSGALGKQSSSLLSRLISSKMPGGFTITVARKYLQTRWGLPSGRQDGVLLVALSNEPAARLGSEADAKAFLDSMAQKYASIVGVDLSSAASASGAAGAGAAAGAAMIDAGALEEITKDHKVLARQQLQVLARYLKMDLDNGERKFLKEKDTVAELQAQLDYLNAELGEFFVNGVATSFSRKKARTFDSSWNWAKQSLLSLYFEIIHGVLKNVDREVVSEAINIMNRSNDALIKFMEYHISNTDETKGENYQLVKTLGEQLIENCKQVLDVDPVYKDVAKPTGPKTAIDKNGNITYSEEPREKVRKLSQYVQEMALGGPITKESQPTIEEDLTRVYKAISAQADKQDISSSTRVEFEKLYSDLMKFLESSKEIDPSQTTQLAGMDVEDALDKDSTKEVASLPNKSTISKTVSSTIPRETIPFLHLRKKTPAGDWKYDRQLSSLFLDGLEKAAFNGVTFKDKYVLITGAGKGSIGAEVLQGLLQGGAKVVVTTSRFSKQVTDYYQSIYAKYGAKGSTLIVVPFNQGSKQDVEALIEFIYDTEKNGGLGWDLDAIIPFAAIPEQGIELEHIDSKSEFAHRIMLTNILRMMGCVKKQKSARGIETRPAQVILPMSPNHGTFGGDGMYSESKLSLETLFNRWHSESWANQLTVCGAIIGWTRGTGLMSANNIIAEGIEKMGVRTFSQKEMAFNLLGLLTPEVVELCQKSPVMADLNGGLQFVPELKEFTAKLRKELVETSEVRKAVSIETALEHKVVNGNSADAAYAQVEIQPRANIQLDFPELKPYKQVKQIAPAELEGLLDLERVIVVTGFAEVGPWGSARTRWEMEAFGEFSLEGCVEMAWIMGFISYHNGNLKGRPYTGWVDSKTKEPVDDKDVKAKYETSILEHSGIRLIEPELFNGYNPEKKEMIQEVIVEEDLEPFEASKETAEQFKHQHGDKVDIFEIPETGEYSVKLLKGATLYIPKALRFDRLVAGQIPTGWNAKTYGISDDIISQVDPITLFVLVSVVEAFIASGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGMFKDRFKDEPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATSVESVDIGVETILSGKARICIVGGYDDFQEEGSFEFGNMKATSNTLEEFEHGRTPAEMSRPATTTRNGFMEAQGAGIQIIMQADLALKMGVPIYGIVAMAATATDKIGRSVPAPGKGILTTAREHHSSVKYASPNLNMKYRKRQLVTREAQIKDWVENELEALKLEAEEIPSEDQNEFLLERTREIHNEAESQLRAAQQQWGNDFYKRDPRIAPLRGALATYGLTIDDLGVASFHGTSTKANDKNESATINEMMKHLGRSEGNPVIGVFQKFLTGHPKGAAGAWMMNGALQILNSGIIPGNRNADNVDKILEQFEYVLYPSKTLKTDGVRAVSITSFGFGQKGGQAIVVHPDYLYGAITEDRYNEYVAKVSAREKSAYKFFHNGMIYNKLFVSKEHAPYTDELEEDVYLDPLARVSKDKKSGSLTFNSKNIQSKDSYINANTIETAKMIENMTKEKVSNGGVGVDVELITSINVENDTFIERNFTPQEIEYCSAQPSVQSSFAGTWSAKEAVFKSLGVKSLGGGAALKDIEIVRVNKNAPAVELHGNAKKAAEEAGVTDVKVSISHDDLQAVAVAVSTKK

Target ORF information:

RefSeq Version	NM_001184045.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C trifunctional fatty acid synthase subunit FAS2 (FAS2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184045.1

ORF Insert Sequence:

ATGAAGCCGG AAGTTGAGCA AGAATTAGCT CATATTTTGC TAACTGAATT GTTAGCTTAT 
CAATTTGCCT CTCCTGTGAG ATGGATTGAA ACTCAAGATG TTTTTTTGAA GGATTTTAAC 
ACTGAAAGGG TTGTTGAAAT CGGTCCTTCT CCAACTTTGG CTGGGATGGC TCAAAGAACC 
TTGAAGAATA AATACGAATC TTACGATGCT GCTCTGTCTT TACATAGAGA AATCTTATGC 
TATTCGAAGG ATGCCAAAGA GATTTATTAT ACCCCAGATC CATCCGAACT AGCTGCAAAG 
GAAGAGCCCG CTAAGGAAGA AGCTCCTGCT CCAACTCCAG CTGCTAGTGC TCCTGCTCCT 
GCAGCAGCAG CCCCAGCTCC CGTCGCGGCA GCAGCCCCAG CTGCAGCAGC TGCTGAGATT 
GCCGATGAAC CTGTCAAGGC TTCCCTATTG TTGCACGTTT TGGTTGCTCA CAAGTTGAAG 
AAGTCGTTAG ATTCCATTCC AATGTCCAAG ACAATCAAAG ACTTGGTCGG TGGTAAATCT 
ACAGTCCAAA ATGAAATTTT GGGTGATTTA GGTAAAGAAT TTGGTACTAC TCCTGAAAAA 
CCAGAAGAAA CTCCATTAGA AGAATTGGCA GAAACTTTCC AAGATACCTT CTCTGGAGCA 
TTGGGTAAGC AATCTTCCTC GTTATTATCA AGATTAATCT CATCTAAGAT GCCTGGTGGG 
TTTACTATTA CTGTCGCTAG AAAATACTTA CAAACTCGCT GGGGACTACC ATCTGGTAGA 
CAAGATGGTG TCCTTTTGGT AGCTTTATCT AACGAGCCTG CTGCTCGTCT AGGTTCTGAA 
GCTGATGCCA AGGCTTTCTT GGACTCCATG GCTCAAAAAT ACGCTTCCAT TGTTGGTGTT 
GACTTATCAT CAGCTGCTAG CGCTAGTGGT GCTGCCGGTG CAGGTGCTGC TGCCGGTGCA 
GCTATGATCG ATGCTGGCGC TCTGGAAGAA ATAACCAAAG ACCACAAGGT TTTGGCGCGT 
CAACAACTGC AAGTATTGGC TCGTTATCTA AAAATGGACT TGGATAACGG TGAAAGAAAG 
TTCTTGAAAG AAAAGGACAC TGTTGCTGAA CTTCAAGCTC AGTTGGATTA CTTGAATGCC 
GAATTAGGTG AATTCTTTGT TAACGGTGTT GCTACTTCTT TCTCTAGAAA AAAGGCCAGA 
ACCTTCGATT CTTCCTGGAA CTGGGCTAAA CAATCTTTAT TATCATTATA CTTTGAGATA 
ATTCATGGTG TCTTGAAAAA CGTTGATAGA GAGGTTGTTA GTGAAGCTAT CAATATCATG 
AACAGATCTA ACGATGCTTT GATTAAATTC ATGGAATACC ATATCTCTAA CACTGATGAA 
ACAAAAGGTG AAAACTATCA ATTGGTTAAA ACTCTTGGTG AGCAGTTGAT TGAAAACTGT 
AAACAAGTTT TGGATGTTGA TCCAGTTTAC AAAGATGTTG CTAAGCCTAC CGGTCCAAAA 
ACTGCTATTG ACAAGAACGG TAACATTACA TACTCAGAAG AGCCAAGAGA AAAGGTTAGG 
AAATTATCTC AATACGTACA AGAAATGGCC CTTGGTGGTC CAATCACCAA AGAATCTCAA 
CCTACTATTG AAGAGGATTT GACTCGTGTT TACAAGGCAA TCAGTGCTCA AGCTGATAAA 
CAAGATATTT CCAGCTCCAC CAGGGTTGAA TTTGAAAAAC TATATAGTGA TTTGATGAAG 
TTCTTGGAAA GCTCCAAAGA AATCGATCCT TCTCAAACAA CCCAATTGGC CGGTATGGAT 
GTTGAGGATG CTTTGGACAA AGATTCCACC AAAGAAGTTG CTTCTTTGCC AAACAAATCT 
ACCATTTCTA AGACGGTATC TTCAACTATT CCAAGAGAAA CTATTCCGTT CTTACATTTG 
AGAAAGAAGA CTCCTGCCGG AGATTGGAAA TATGACCGCC AATTGTCTTC TCTTTTCTTA 
GATGGTTTAG AAAAGGCTGC CTTCAACGGT GTCACCTTCA AGGACAAATA CGTCTTGATC 
ACTGGTGCTG GTAAGGGTTC TATTGGTGCT GAAGTCTTGC AAGGTTTGTT ACAAGGTGGT 
GCTAAGGTTG TTGTTACCAC CTCTCGTTTC TCTAAGCAAG TTACAGACTA CTACCAATCC 
ATTTACGCCA AATATGGTGC TAAGGGTTCT ACTTTGATTG TTGTTCCATT CAACCAAGGT 
TCTAAGCAAG ACGTTGAAGC TTTGATTGAA TTTATCTACG ACACTGAAAA GAATGGTGGT 
TTAGGTTGGG ATCTAGATGC TATTATTCCA TTCGCGGCCA TTCCAGAACA AGGTATTGAA 
TTAGAACATA TTGATTCTAA GTCTGAATTT GCTCATAGAA TCATGTTGAC CAATATCTTA 
AGAATGATGG GTTGTGTCAA GAAGCAAAAA TCTGCAAGAG GTATTGAAAC AAGACCAGCT 
CAAGTCATTC TACCAATGTC TCCAAACCAT GGTACTTTCG GTGGTGATGG TATGTATTCA 
GAATCCAAGT TGTCTTTGGA AACTTTGTTC AACAGATGGC ACTCTGAATC CTGGGCCAAT 
CAATTAACCG TTTGCGGTGC TATTATTGGT TGGACTAGAG GTACTGGTTT AATGAGCGCT 
AATAACATCA TTGCTGAAGG CATTGAAAAG ATGGGTGTTC GTACTTTCTC TCAAAAGGAA 
ATGGCTTTCA ACTTATTGGG TCTATTGACT CCAGAAGTCG TAGAATTGTG CCAAAAATCA 
CCTGTTATGG CTGACTTGAA TGGTGGTTTG CAATTTGTTC CTGAATTGAA GGAATTCACT 
GCTAAATTGC GTAAAGAGTT GGTTGAAACT TCTGAAGTTA GAAAGGCAGT TTCCATCGAA 
ACTGCTTTGG AGCATAAGGT TGTCAATGGC AATAGCGCTG ATGCTGCATA TGCTCAAGTC 
GAAATTCAAC CAAGAGCTAA CATTCAACTG GACTTCCCAG AATTGAAACC ATACAAACAG 
GTTAAACAAA TTGCTCCCGC TGAGCTTGAA GGTTTGTTGG ATTTGGAAAG AGTTATTGTA 
GTTACCGGTT TTGCTGAAGT CGGCCCATGG GGTTCGGCCA GAACAAGATG GGAAATGGAA 
GCTTTTGGTG AATTTTCGTT GGAAGGTTGC GTTGAAATGG CCTGGATTAT GGGCTTCATT 
TCATACCATA ACGGTAATTT GAAGGGTCGT CCATACACTG GTTGGGTTGA TTCCAAAACA 
AAAGAACCAG TTGATGACAA GGACGTTAAG GCCAAGTATG AAACATCAAT CCTAGAACAC 
AGTGGTATCA GATTGATCGA ACCAGAGTTA TTCAATGGTT ACAACCCAGA AAAGAAGGAA 
ATGATTCAAG AAGTCATTGT CGAAGAAGAC TTGGAACCAT TTGAGGCTTC GAAGGAAACT 
GCCGAACAAT TTAAACACCA ACATGGTGAC AAAGTGGATA TCTTCGAAAT CCCAGAAACA 
GGAGAGTACT CTGTTAAGTT ACTAAAGGGT GCCACTTTAT ACATTCCAAA GGCTTTGAGA 
TTTGACCGTT TGGTTGCAGG TCAAATTCCA ACTGGTTGGA ATGCTAAGAC TTATGGTATC 
TCTGATGATA TCATTTCTCA GGTTGACCCA ATCACATTAT TCGTTTTGGT CTCTGTTGTG 
GAAGCATTTA TTGCATCTGG TATCACCGAC CCATACGAAA TGTACAAATA CGTACATGTT 
TCTGAGGTTG GTAACTGTTC TGGTTCTGGT ATGGGTGGTG TTTCTGCCTT ACGTGGTATG 
TTTAAGGACC GTTTCAAGGA TGAGCCTGTC CAAAATGATA TTTTACAAGA ATCATTTATC 
AACACCATGT CCGCTTGGGT TAATATGTTG TTGATTTCCT CATCTGGTCC AATCAAGACA 
CCTGTTGGTG CCTGTGCCAC ATCCGTGGAA TCTGTTGACA TTGGTGTAGA AACCATCTTG 
TCTGGTAAGG CTAGAATCTG TATTGTCGGT GGTTACGATG ATTTCCAAGA AGAAGGCTCC 
TTTGAGTTCG GTAACATGAA GGCCACTTCC AACACTTTGG AAGAATTTGA ACATGGTCGT 
ACCCCAGCGG AAATGTCCAG ACCTGCCACC ACTACCCGTA ACGGTTTTAT GGAAGCTCAA 
GGTGCTGGTA TTCAAATCAT CATGCAAGCT GATTTAGCTT TGAAGATGGG TGTGCCAATT 
TACGGTATTG TTGCCATGGC TGCTACCGCC ACCGATAAGA TTGGTAGATC TGTGCCAGCT 
CCAGGTAAGG GTATTTTAAC CACTGCTCGT GAACACCACT CCAGTGTTAA GTATGCTTCA 
CCAAACTTGA ACATGAAGTA CAGAAAGCGC CAATTGGTTA CTCGTGAAGC TCAGATTAAA 
GATTGGGTAG AAAACGAATT GGAAGCTTTG AAGTTGGAGG CCGAAGAAAT TCCAAGCGAA 
GACCAAAACG AGTTCTTACT TGAACGTACC AGAGAAATCC ACAACGAAGC TGAAAGTCAA 
TTGAGAGCTG CACAACAACA ATGGGGTAAC GACTTCTACA AGAGGGACCC ACGTATTGCT 
CCATTGAGAG GAGCACTGGC TACTTACGGT TTAACTATTG ATGACTTGGG TGTCGCTTCA 
TTCCACGGTA CATCCACAAA GGCTAATGAC AAGAACGAAT CTGCCACAAT TAATGAAATG 
ATGAAGCATT TGGGTAGATC TGAAGGTAAT CCCGTCATTG GTGTTTTCCA AAAGTTCTTG 
ACTGGTCATC CAAAGGGTGC TGCTGGTGCA TGGATGATGA ATGGTGCTTT GCAAATTCTA 
AACAGTGGTA TTATTCCAGG TAACCGTAAC GCTGATAACG TGGATAAGAT CTTGGAGCAA 
TTTGAATACG TCTTGTACCC ATCCAAGACT TTAAAGACCG ACGGTGTCAG AGCCGTGTCC 
ATCACTTCTT TCGGTTTTGG TCAAAAGGGT GGTCAAGCTA TTGTGGTTCA TCCAGACTAC 
TTATACGGTG CTATCACTGA AGACAGATAC AACGAGTATG TCGCCAAGGT TAGTGCCAGA 
GAGAAAAGTG CCTACAAATT CTTCCATAAT GGTATGATCT ACAACAAGTT GTTCGTAAGT 
AAAGAGCATG CTCCATACAC TGATGAATTG GAAGAGGATG TTTACTTGGA CCCATTAGCC 
CGTGTATCTA AGGATAAGAA ATCAGGCTCC TTGACTTTCA ACTCTAAAAA CATCCAAAGC 
AAGGACAGTT ACATCAATGC TAACACCATT GAAACTGCCA AGATGATTGA AAACATGACC 
AAGGAGAAAG TCTCTAACGG TGGCGTCGGT GTAGATGTTG AATTAATCAC TAGCATCAAC 
GTTGAAAATG ATACTTTTAT CGAGCGCAAT TTCACCCCGC AAGAAATAGA GTACTGCAGC 
GCGCAGCCTA GTGTGCAAAG CTCTTTCGCT GGGACATGGT CCGCCAAAGA GGCTGTTTTC 
AAGTCCTTAG GCGTCAAGTC CTTAGGCGGT GGTGCTGCAT TGAAAGACAT CGAAATCGTA 
CGCGTTAACA AAAACGCTCC AGCCGTTGAA CTGCACGGTA ACGCCAAAAA GGCTGCCGAA 
GAAGCTGGTG TTACCGATGT GAAGGTATCT ATTTCTCACG ATGACCTCCA AGCTGTCGCG 
GTCGCCGTTT CTACTAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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FAS2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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