SEC12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC12
Entrez Gene ID	855760
Full Name	Sar family guanine nucleotide exchange factor SEC12
Synonyms	SED2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183203.1	NP_014423.1	Sar family guanine nucleotide exchange factor SEC12

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SEC12	NP_014423.1
Eremothecium gossypii	AGOS_AAR048W	NP_982589.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structure of Sec12 implicates potassium ion coordination in Sar1 activation.
Title: The structure of Sec12 implicates potassium ion coordination in Sar1 activation.

The following SEC12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04649	NM_001183203.1 Latest version!	Saccharomyces cerevisiae S288C Sar family guanine nucleotide exchange factor SEC12 (SEC12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC12 ( NM_001183203.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014423.1 Saccharomyces cerevisiae S288C Sar family guanine nucleotide exchange factor SEC12 (SEC12), partial mRNA.

Price & Availability↑

CloneID	OSi04649
Clone ID Related Accession (Same CDS sequence)	NM_001183203.1
Accession Version	NM_001183203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Sar family guanine nucleotide exchange factor SEC12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGTTCG TGACAGCTAG TTATAACGTC GGGTATCCTG CGTACGGTGC AAAATTTTTG 
AATAACGACA CATTACTTGT GGCAGGCGGT GGAGGAGAAG GAAACAATGG CATACCAAAC 
AAGCTGACGG TCTTGCGCGT GGATCCTACC AAAGATACTG AGAAGGAACA GTTTCATATA 
TTGAGCGAGT TTGCATTGGA AGACAACGAC GACTCTCCTA CTGCAATTGA CGCTTCCAAG 
GGTATCATTT TGGTTGGCTG CAATGAAAAT AGCACTAAGA TTACCCAAGG TAAAGGTAAT 
AAGCACTTGA GAAAATTTAA ATACGATAAA GTGAATGATC AATTGGAGTT CCTCACTAGT 
GTAGACTTTG ACGCATCTAC AAATGCGGAT GACTACACGA AGCTGGTTTA TATTTCACGA 
GAAGGTACCG TTGCAGCTAT CGCATCATCT AAAGTACCTG CTATAATGAG AATCATTGAC 
CCGAGCGACT TGACAGAGAA GTTTGAGATC GAGACTAGGG GTGAAGTAAA GGATTTACAC 
TTTTCCACTG ATGGTAAGGT TGTTGCTTAT ATCACCGGTT CTAGCTTGGA AGTGATTTCA 
ACAGTGACTG GAAGTTGCAT TGCTAGGAAA ACAGATTTTG ATAAGAATTG GAGTTTATCT 
AAAATAAACT TCATAGCCGA TGACACAGTA TTGATAGCAG CCTCTTTAAA AAAAGGGAAA 
GGTATTGTGC TGACCAAAAT AAGCATCAAA TCAGGAAACA CTTCCGTATT AAGATCCAAA 
CAAGTGACAA ACAGATTCAA AGGGATTACT TCTATGGATG TCGACATGAA GGGTGAATTG 
GCGGTACTGG CAAGTAATGA CAATTCCATA GCTCTTGTGA AACTAAAAGA CCTGTCAATG 
TCTAAAATAT TCAAACAAGC TCATAGTTTT GCCATTACAG AGGTCACTAT CTCTCCGGAC 
TCTACATATG TGGCGAGTGT TTCGGCAGCC AACACTATCC ACATAATAAA ATTACCGCTT 
AACTACGCCA ACTACACCTC AATGAAACAA AAAATCTCTA AATTTTTCAC CAACTTCATC 
CTTATTGTGC TGCTTTCTTA CATTTTACAG TTCTCCTATA AGCACAATTT GCATTCCATG 
CTTTTCAATT ACGCGAAGGA CAATTTTCTA ACGAAAAGAG ACACCATCTC TTCGCCCTAC 
GTAGTTGATG AAGACTTACA TCAAACAACT TTGTTTGGCA ACCACGGTAC AAAAACATCT 
GTACCTAGCG TAGATTCCAT AAAAGTGCAT GGCGTGCATG AGACGAGTTC TGTGAATGGA 
ACTGAAGTCT TATGTACTGA AAGTAACATT ATTAATACTG GAGGGGCAGA GTTTGAGATC 
ACCAACGCAA CTTTTCGAGA AATAGATGAT GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014423.1
CDS	1..1416
Translation	MKFVTASYNVGYPAYGAKFLNNDTLLVAGGGGEGNNGIPNKLTVLRVDPTKDTEKEQFHILSEFALEDNDDSPTAIDASKGIILVGCNENSTKITQGKGNKHLRKFKYDKVNDQLEFLTSVDFDASTNADDYTKLVYISREGTVAAIASSKVPAIMRIIDPSDLTEKFEIETRGEVKDLHFSTDGKVVAYITGSSLEVISTVTGSCIARKTDFDKNWSLSKINFIADDTVLIAASLKKGKGIVLTKISIKSGNTSVLRSKQVTNRFKGITSMDVDMKGELAVLASNDNSIALVKLKDLSMSKIFKQAHSFAITEVTISPDSTYVASVSAANTIHIIKLPLNYANYTSMKQKISKFFTNFILIVLLSYILQFSYKHNLHSMLFNYAKDNFLTKRDTISSPYVVDEDLHQTTLFGNHGTKTSVPSVDSIKVHGVHETSSVNGTEVLCTESNIINTGGAEFEITNATFREIDDA

Target ORF information:

RefSeq Version	NM_001183203.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Sar family guanine nucleotide exchange factor SEC12 (SEC12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183203.1

ORF Insert Sequence:

ATGAAGTTCG TGACAGCTAG TTATAACGTC GGGTATCCTG CGTACGGTGC AAAATTTTTG 
AATAACGACA CATTACTTGT GGCAGGCGGT GGAGGAGAAG GAAACAATGG CATACCAAAC 
AAGCTGACGG TCTTGCGCGT GGATCCTACC AAAGATACTG AGAAGGAACA GTTTCATATA 
TTGAGCGAGT TTGCATTGGA AGACAACGAC GACTCTCCTA CTGCAATTGA CGCTTCCAAG 
GGTATCATTT TGGTTGGCTG CAATGAAAAT AGCACTAAGA TTACCCAAGG TAAAGGTAAT 
AAGCACTTGA GAAAATTTAA ATACGATAAA GTGAATGATC AATTGGAGTT CCTCACTAGT 
GTAGACTTTG ACGCATCTAC AAATGCGGAT GACTACACGA AGCTGGTTTA TATTTCACGA 
GAAGGTACCG TTGCAGCTAT CGCATCATCT AAAGTACCTG CTATAATGAG AATCATTGAC 
CCGAGCGACT TGACAGAGAA GTTTGAGATC GAGACTAGGG GTGAAGTAAA GGATTTACAC 
TTTTCCACTG ATGGTAAGGT TGTTGCTTAT ATCACCGGTT CTAGCTTGGA AGTGATTTCA 
ACAGTGACTG GAAGTTGCAT TGCTAGGAAA ACAGATTTTG ATAAGAATTG GAGTTTATCT 
AAAATAAACT TCATAGCCGA TGACACAGTA TTGATAGCAG CCTCTTTAAA AAAAGGGAAA 
GGTATTGTGC TGACCAAAAT AAGCATCAAA TCAGGAAACA CTTCCGTATT AAGATCCAAA 
CAAGTGACAA ACAGATTCAA AGGGATTACT TCTATGGATG TCGACATGAA GGGTGAATTG 
GCGGTACTGG CAAGTAATGA CAATTCCATA GCTCTTGTGA AACTAAAAGA CCTGTCAATG 
TCTAAAATAT TCAAACAAGC TCATAGTTTT GCCATTACAG AGGTCACTAT CTCTCCGGAC 
TCTACATATG TGGCGAGTGT TTCGGCAGCC AACACTATCC ACATAATAAA ATTACCGCTT 
AACTACGCCA ACTACACCTC AATGAAACAA AAAATCTCTA AATTTTTCAC CAACTTCATC 
CTTATTGTGC TGCTTTCTTA CATTTTACAG TTCTCCTATA AGCACAATTT GCATTCCATG 
CTTTTCAATT ACGCGAAGGA CAATTTTCTA ACGAAAAGAG ACACCATCTC TTCGCCCTAC 
GTAGTTGATG AAGACTTACA TCAAACAACT TTGTTTGGCA ACCACGGTAC AAAAACATCT 
GTACCTAGCG TAGATTCCAT AAAAGTGCAT GGCGTGCATG AGACGAGTTC TGTGAATGGA 
ACTGAAGTCT TATGTACTGA AAGTAACATT ATTAATACTG GAGGGGCAGA GTTTGAGATC 
ACCAACGCAA CTTTTCGAGA AATAGATGAT GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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