APP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	APP1
Entrez Gene ID	855630
Full Name	phosphatidate phosphatase APP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182932.1	NP_014305.1	phosphatidate phosphatase APP1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C13651g	XP_452809.1
Eremothecium gossypii	AGOS_ACL081C	NP_983323.2
Saccharomyces cerevisiae (baker's yeast)	APP1	NP_014305.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

App1p also utilized diacylglycerol pyrophosphate and lyso-PA as substrates with specificity constants 4- and 7-fold lower, respectively, when compared with PA.
Title: Characterization of the yeast actin patch protein App1p phosphatidate phosphatase.

App1p is a phosphatidate phosphatase enzyme.
Title: The Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme.

Pus4 and App1 dramatically inhibit Brome Mosaic Virus systemic spread in plants, reduce BMV genomic plus-strand RNA accumulation, and prevent the encapsidation of a BMV RNA.
Title: RNA-binding proteins that inhibit RNA virus infection.

The following APP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04538	NM_001182932.1 Latest version!	Saccharomyces cerevisiae S288C phosphatidate phosphatase APP1 (APP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APP1 ( NM_001182932.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014305.1 Saccharomyces cerevisiae S288C phosphatidate phosphatase APP1 (APP1), partial mRNA.

Price & Availability↑

CloneID	OSi04538
Clone ID Related Accession (Same CDS sequence)	NM_001182932.1
Accession Version	NM_001182932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphatidate phosphatase APP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATAGTC AAGGTTACGA TGAAAGCTCT TCCTCTACTG CTGCTACTAG CGGTCCTACT 
TCTGGAGACC CACGAATGGG TAAGAAACAA CGGTTTATGA ATCTCATAAG AACCACAAAG 
GATGTTTATA TTCCTAACCT GACGTCATCC ATTTCTCAAA AGACAATGGA TGGCATCAGG 
AGCACTACGA ATTCCTTCGA AGGTTATAAT GACTTGCCTA TGGAATTGCC ACATAATACC 
ACGATAACAT ATTTTCCAAC GTATACCACA ACTAATTTGG TTGACCCTGA CGGTTTGAGT 
GCGCCTCGGA AAGATTTTGA AACTACTGTC AGATGTGCTG TTTCTTATCC AGGAAATCCT 
ACAAGCAGGA GAAATAGGTG GTTGCTTTCT CTTTGTAAAC AATATCTTAG AACTGGAACG 
GCGGAGGCCG ATGTTGCACC TGTTGTTCCC CCTCATCTCG AAGAGGATTC TGGAGATTTA 
AACGACTCCC AATCAAGTAT AGAAAGTTCT TTGTCTTCAA AGAGCGAAAA TAGGTATTCA 
CATATGGGCA TCCAAGAGGA AGATGTTCTG AATGAGAGAA TTCAAGGATT CCTGTCCAAA 
AAAGTTCCTA ATACTCCAGT GGTGGTTGAC CTATTGCCCA AAGATAAGCT CAGAGGGGAT 
ACGGCTTCCT TTTTTGGTAC CACGGACTCT TATGGTAATT TACTAATCAA GGCAGAAACA 
GATTTTTTAC CGTCCAAGAT TAATATTACA TTAGATACCC CTATAGAAGG CCATGCAGAT 
CCCATATCTG AAACATTTCC GGCCAATTAT GTCTCGCCCT ACGGTATTGG TTTAATCAGT 
GATATTGACG ACACCATCAA GCATACTGGC GTTACGGGTG ACAGGAGATC GATGTTTCGT 
AATGTTTTCA TCCATGATGT TCAATCGTGG GTAATAGATG GTGTTCCTCT TTGGTACAAA 
ACTTTACATG ATGTCGCAGA CGTTGATTTC TTTTATGTTT CTAATTCACC CATCCAAACT 
TTTACTCTTT TGAAACAATA TATTTGCGCC AATTTTCCTC CGGGTCCAAT TTTTTTGAAA 
CAATACTCTG GTAATTTCTT TTCCACTATC ATGACTTCAA GTGCGAATAG AAAAATTCAG 
CCGATTGCAA ATATCTTAAA GGATTTTCCC AAAAAAAAAT TTATTTTGGT CGGTGATTCT 
GGTGAACATG ACTTAGAAGC GTATACCACT ACGGCTTTAC AATTTCCGAA CCAGATTCTG 
GCTATTTATA TAAGATGTTG CTCTAATTCC ATGAGTGATG TCCCATCGCA TGATGAAGAA 
GTTATGAATG AAGTGAACAA TATCATCGAA TTACAACAAC GGCCCATGCA AATGACCAAG 
TCTACCGTAC GTACCAGGAG AAGACCACCA CCACCTCCCA TTCCTTCCAC TCAAAAGCCA 
TCGTTGACAG AAGAGCAAAC CGAAAGTATA AGAATGTCAC GCCGTAATAA AGATGAGAAC 
AATGCTAAAA GAGTTGCCCC ACCACCGTTG CCGAACAGAC AGTTACCTAA CTTAGACGCT 
AATACTTACT ATGTACCATC CTCACAAAAT GACTATGGCA TGTATGGTGC TTTCATGGAT 
AAAAAAGCAG ATGAATGGAA GAGAAGAGTA ATGGACTCAA TTCAAAAATT GAGCAATCAA 
GACACCACAT TGATGTTCTT TTCGGATCCG GCATTAAGTT TGGAAGACAG TATTCGCAGA 
ATTAGGGAGA AGTATTCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014305.1
CDS	1..1764
Translation	MNSQGYDESSSSTAATSGPTSGDPRMGKKQRFMNLIRTTKDVYIPNLTSSISQKTMDGIRSTTNSFEGYNDLPMELPHNTTITYFPTYTTTNLVDPDGLSAPRKDFETTVRCAVSYPGNPTSRRNRWLLSLCKQYLRTGTAEADVAPVVPPHLEEDSGDLNDSQSSIESSLSSKSENRYSHMGIQEEDVLNERIQGFLSKKVPNTPVVVDLLPKDKLRGDTASFFGTTDSYGNLLIKAETDFLPSKINITLDTPIEGHADPISETFPANYVSPYGIGLISDIDDTIKHTGVTGDRRSMFRNVFIHDVQSWVIDGVPLWYKTLHDVADVDFFYVSNSPIQTFTLLKQYICANFPPGPIFLKQYSGNFFSTIMTSSANRKIQPIANILKDFPKKKFILVGDSGEHDLEAYTTTALQFPNQILAIYIRCCSNSMSDVPSHDEEVMNEVNNIIELQQRPMQMTKSTVRTRRRPPPPPIPSTQKPSLTEEQTESIRMSRRNKDENNAKRVAPPPLPNRQLPNLDANTYYVPSSQNDYGMYGAFMDKKADEWKRRVMDSIQKLSNQDTTLMFFSDPALSLEDSIRRIREKYSN

Target ORF information:

RefSeq Version	NM_001182932.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphatidate phosphatase APP1 (APP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182932.1

ORF Insert Sequence:

ATGAATAGTC AAGGTTACGA TGAAAGCTCT TCCTCTACTG CTGCTACTAG CGGTCCTACT 
TCTGGAGACC CACGAATGGG TAAGAAACAA CGGTTTATGA ATCTCATAAG AACCACAAAG 
GATGTTTATA TTCCTAACCT GACGTCATCC ATTTCTCAAA AGACAATGGA TGGCATCAGG 
AGCACTACGA ATTCCTTCGA AGGTTATAAT GACTTGCCTA TGGAATTGCC ACATAATACC 
ACGATAACAT ATTTTCCAAC GTATACCACA ACTAATTTGG TTGACCCTGA CGGTTTGAGT 
GCGCCTCGGA AAGATTTTGA AACTACTGTC AGATGTGCTG TTTCTTATCC AGGAAATCCT 
ACAAGCAGGA GAAATAGGTG GTTGCTTTCT CTTTGTAAAC AATATCTTAG AACTGGAACG 
GCGGAGGCCG ATGTTGCACC TGTTGTTCCC CCTCATCTCG AAGAGGATTC TGGAGATTTA 
AACGACTCCC AATCAAGTAT AGAAAGTTCT TTGTCTTCAA AGAGCGAAAA TAGGTATTCA 
CATATGGGCA TCCAAGAGGA AGATGTTCTG AATGAGAGAA TTCAAGGATT CCTGTCCAAA 
AAAGTTCCTA ATACTCCAGT GGTGGTTGAC CTATTGCCCA AAGATAAGCT CAGAGGGGAT 
ACGGCTTCCT TTTTTGGTAC CACGGACTCT TATGGTAATT TACTAATCAA GGCAGAAACA 
GATTTTTTAC CGTCCAAGAT TAATATTACA TTAGATACCC CTATAGAAGG CCATGCAGAT 
CCCATATCTG AAACATTTCC GGCCAATTAT GTCTCGCCCT ACGGTATTGG TTTAATCAGT 
GATATTGACG ACACCATCAA GCATACTGGC GTTACGGGTG ACAGGAGATC GATGTTTCGT 
AATGTTTTCA TCCATGATGT TCAATCGTGG GTAATAGATG GTGTTCCTCT TTGGTACAAA 
ACTTTACATG ATGTCGCAGA CGTTGATTTC TTTTATGTTT CTAATTCACC CATCCAAACT 
TTTACTCTTT TGAAACAATA TATTTGCGCC AATTTTCCTC CGGGTCCAAT TTTTTTGAAA 
CAATACTCTG GTAATTTCTT TTCCACTATC ATGACTTCAA GTGCGAATAG AAAAATTCAG 
CCGATTGCAA ATATCTTAAA GGATTTTCCC AAAAAAAAAT TTATTTTGGT CGGTGATTCT 
GGTGAACATG ACTTAGAAGC GTATACCACT ACGGCTTTAC AATTTCCGAA CCAGATTCTG 
GCTATTTATA TAAGATGTTG CTCTAATTCC ATGAGTGATG TCCCATCGCA TGATGAAGAA 
GTTATGAATG AAGTGAACAA TATCATCGAA TTACAACAAC GGCCCATGCA AATGACCAAG 
TCTACCGTAC GTACCAGGAG AAGACCACCA CCACCTCCCA TTCCTTCCAC TCAAAAGCCA 
TCGTTGACAG AAGAGCAAAC CGAAAGTATA AGAATGTCAC GCCGTAATAA AGATGAGAAC 
AATGCTAAAA GAGTTGCCCC ACCACCGTTG CCGAACAGAC AGTTACCTAA CTTAGACGCT 
AATACTTACT ATGTACCATC CTCACAAAAT GACTATGGCA TGTATGGTGC TTTCATGGAT 
AAAAAAGCAG ATGAATGGAA GAGAAGAGTA ATGGACTCAA TTCAAAAATT GAGCAATCAA 
GACACCACAT TGATGTTCTT TTCGGATCCG GCATTAAGTT TGGAAGACAG TATTCGCAGA 
ATTAGGGAGA AGTATTCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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APP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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