TRF5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TRF5
Entrez Gene ID	855417
Full Name	non-canonical poly(A) polymerase TRF5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183137.1	NP_014100.2	non-canonical poly(A) polymerase TRF5

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A07359g	XP_451327.1
Saccharomyces cerevisiae (baker's yeast)	TRF5	NP_014100.2
Eremothecium gossypii	AGOS_AEL207W	NP_984654.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cotranscriptional recruitment of RNA exosome cofactors Rrp47p and Mpp6p and two distinct Trf-Air-Mtr4 polyadenylation (TRAMP) complexes assists the exonuclease Rrp6p in the targeting and degradation of an aberrant messenger
Title: Cotranscriptional recruitment of RNA exosome cofactors Rrp47p and Mpp6p and two distinct Trf-Air-Mtr4 polyadenylation (TRAMP) complexes assists the exonuclease Rrp6p in the targeting and degradation of an aberrant messenger ribonucleoprotein particle (mRNP) in yeast.

Air1 zinc knuckles 4 and 5 and a conserved IWRXY motif are critical for the function and integrity of the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) RNA quality control complex.
Title: Air1 zinc knuckles 4 and 5 and a conserved IWRXY motif are critical for the function and integrity of the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) RNA quality control complex.

Study demonstrates that TRF4, the exosome, and TRF5 participate in antisense RNA-mediated regulation of genes involved in phosphate metabolism.
Title: Distinct roles of non-canonical poly(A) polymerases in RNA metabolism.

Trf5 colocalized with nascent pre-rRNPs, indicating that this nucleolar surveillance initiates cotranscriptionally.
Title: The nuclear poly(A) polymerase and Exosome cofactor Trf5 is recruited cotranscriptionally to nucleolar surveillance.

TRF4, TRF5, and RRP6 play a role in the regulation of histone mRNA levels in yeast.
Title: Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae.

The following TRF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04352	NM_001183137.1 Latest version!	Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase TRF5 (TRF5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRF5 ( NM_001183137.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014100.2 Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase TRF5 (TRF5), partial mRNA.

Price & Availability↑

CloneID	OSi04352
Clone ID Related Accession (Same CDS sequence)	NM_001183137.1
Accession Version	NM_001183137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	non-canonical poly(A) polymerase TRF5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAAGGC TCAAAGCAAA ATATTCACCT ACAAAAGGTA AAAGAAAAGA AGATAAACAT 
ACAAAACGTA TGCGTAAATC ATCCTTCACA AGAACGCAAA AAATGCTTGA AGTATTTAAC 
GATAATCGCT CGCATTTTAA CAAGTACGAG AGCCTCGCTA TCGACGTTGA AGACGATGAC 
ACTTTCGGAA ACCTAGTCTT AATGGAAAAT GATAAAAGTG ATGTCGACAT ACCTGTAATA 
GAAGAAGTCA CATCAAGTGA AGATGAACAA AGGGCAGAAT CTAGCAAAAG AAACAATTCT 
CTCGAAGATA ATCAAGATTT TATTGCTTTT TCGGACAGCT CTGAAGATGA AACAGAACAA 
ATAAAGGAAG ATGATGATGA AAGAAGCAGT TTTCTGTTGA CCGATGAACA TGAAGTTTCT 
AAGCTTACTT CACAGCAATC TCTGAACACT GAAAGCGCTT GCAATGTGGA GTATCCTTGG 
ATAAGAAATC ATTGTCATTC GAAACAAAGG AGAATAGCAG ATTGGCTAAC TTCAGAAATC 
AAAGATTTCG TTCATTATAT CTCTCCAAGC AAGAACGAAA TCAAGTGCAG AAATAGAACG 
ATCGATAAAC TACGCCGGGC GGTCAAGGAA CTGTGGTCGG ATGCAGATTT GCATGTGTTT 
GGTTCTTTTG CCACCGATTT ATATTTGCCG GGTTCTGACA TTGACTGTGT CGTAAACAGC 
CGGAATCGCG ATAAAGAGGA CAGAAATTAC ATTTATGAAT TGGCGAGACA TTTGAAAAAC 
AAAGGGCTAG CTATAAGAAT GGAGGTTATT GTTAAAACGC GAGTGCCTAT TATCAAATTC 
ATAGAGCCCC AATCACAGTT ACATATCGAC GTTTCATTTG AAAGAACGAA CGGGTTAGAG 
GCTGCAAAAC TCATCAGGGA ATGGCTAAGA GACTCTCCAG GGTTACGTGA ACTGGTACTG 
ATAATAAAAC AATTTTTGCA TTCAAGACGA TTGAACAATG TGCACACAGG AGGATTGGGA 
GGTTTCACAG TAATCTGTTT GGTTTATTCT TTTTTAAATA TGCATCCGCG AATAAAATCA 
AACGATATCG ACGTTCTAGA TAACCTGGGT GTTCTACTGA TTGACTTCTT CGAACTTTAC 
GGAAAAAATT TTGGTTATGA TGACGTTGCT ATAAGCATAT CTGATGGTTA CGCCTCTTAT 
ATTCCTAAAT CGTGTTGGAG AACTTTGGAA CCTTCAAGGA GTAAATTCTC GCTAGCTATT 
CAAGATCCTG GTGATCCTAA CAATAATATA AGCAGAGGCT CCTTTAATAT GAAGGATATA 
AAAAAGGCCT TTGCTGGTGC CTTCGAACTG TTGGTCAATA AATGCTGGGA ATTGAATTCT 
GCTACATTCA AAGACCGCGT TGGAAAAAGT ATACTCGGAA ACGTGATCAA ATACCGTGGG 
CAAAAGAGAG ATTTCAACGA CGAAAGAGAT TTAGTGCAGA ATAAAGCCAT CATTGAAAAC 
GAGCGCTACC ACAAGAGGCG CACGAGGATA GTTCAAGAAG ATTTATTTAT AAACGACACC 
GAAGACTTGC CAGTAGAGGA GATCTACAAA CTTGACGAAC CTGCAAAGAA GAAACAGAAG 
GCAAAAAAGG ATAAGAGAGA AGGGGAAATC AAAAAGAGTG CCATTCCATC TCCACCTCCG 
GATTTTGGAG TGAGCAGAAG CAAATTGAAA CGCAAAGTGA AAAAAACTGA TCAGGGCTCT 
CTCCTACACC AAAACAACCT CAGCATAGAT GACTTGATGG GACTTTCTGA GAATGATCAA 
GAATCCGATC AAGATCAAAA AGGAAGAGAT ACCCCTTCGG GACAAGATGA GAAATCACCA 
CTGGAAACTA AGACAGTAGA TGCTCAAACG AGAAGGGACT ACTGGCTCTC TAAAGGCCAG 
GCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014100.2
CDS	1..1929
Translation	MTRLKAKYSPTKGKRKEDKHTKRMRKSSFTRTQKMLEVFNDNRSHFNKYESLAIDVEDDDTFGNLVLMENDKSDVDIPVIEEVTSSEDEQRAESSKRNNSLEDNQDFIAFSDSSEDETEQIKEDDDERSSFLLTDEHEVSKLTSQQSLNTESACNVEYPWIRNHCHSKQRRIADWLTSEIKDFVHYISPSKNEIKCRNRTIDKLRRAVKELWSDADLHVFGSFATDLYLPGSDIDCVVNSRNRDKEDRNYIYELARHLKNKGLAIRMEVIVKTRVPIIKFIEPQSQLHIDVSFERTNGLEAAKLIREWLRDSPGLRELVLIIKQFLHSRRLNNVHTGGLGGFTVICLVYSFLNMHPRIKSNDIDVLDNLGVLLIDFFELYGKNFGYDDVAISISDGYASYIPKSCWRTLEPSRSKFSLAIQDPGDPNNNISRGSFNMKDIKKAFAGAFELLVNKCWELNSATFKDRVGKSILGNVIKYRGQKRDFNDERDLVQNKAIIENERYHKRRTRIVQEDLFINDTEDLPVEEIYKLDEPAKKKQKAKKDKREGEIKKSAIPSPPPDFGVSRSKLKRKVKKTDQGSLLHQNNLSIDDLMGLSENDQESDQDQKGRDTPSGQDEKSPLETKTVDAQTRRDYWLSKGQAL

Target ORF information:

RefSeq Version	NM_001183137.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase TRF5 (TRF5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183137.1

ORF Insert Sequence:

ATGACAAGGC TCAAAGCAAA ATATTCACCT ACAAAAGGTA AAAGAAAAGA AGATAAACAT 
ACAAAACGTA TGCGTAAATC ATCCTTCACA AGAACGCAAA AAATGCTTGA AGTATTTAAC 
GATAATCGCT CGCATTTTAA CAAGTACGAG AGCCTCGCTA TCGACGTTGA AGACGATGAC 
ACTTTCGGAA ACCTAGTCTT AATGGAAAAT GATAAAAGTG ATGTCGACAT ACCTGTAATA 
GAAGAAGTCA CATCAAGTGA AGATGAACAA AGGGCAGAAT CTAGCAAAAG AAACAATTCT 
CTCGAAGATA ATCAAGATTT TATTGCTTTT TCGGACAGCT CTGAAGATGA AACAGAACAA 
ATAAAGGAAG ATGATGATGA AAGAAGCAGT TTTCTGTTGA CCGATGAACA TGAAGTTTCT 
AAGCTTACTT CACAGCAATC TCTGAACACT GAAAGCGCTT GCAATGTGGA GTATCCTTGG 
ATAAGAAATC ATTGTCATTC GAAACAAAGG AGAATAGCAG ATTGGCTAAC TTCAGAAATC 
AAAGATTTCG TTCATTATAT CTCTCCAAGC AAGAACGAAA TCAAGTGCAG AAATAGAACG 
ATCGATAAAC TACGCCGGGC GGTCAAGGAA CTGTGGTCGG ATGCAGATTT GCATGTGTTT 
GGTTCTTTTG CCACCGATTT ATATTTGCCG GGTTCTGACA TTGACTGTGT CGTAAACAGC 
CGGAATCGCG ATAAAGAGGA CAGAAATTAC ATTTATGAAT TGGCGAGACA TTTGAAAAAC 
AAAGGGCTAG CTATAAGAAT GGAGGTTATT GTTAAAACGC GAGTGCCTAT TATCAAATTC 
ATAGAGCCCC AATCACAGTT ACATATCGAC GTTTCATTTG AAAGAACGAA CGGGTTAGAG 
GCTGCAAAAC TCATCAGGGA ATGGCTAAGA GACTCTCCAG GGTTACGTGA ACTGGTACTG 
ATAATAAAAC AATTTTTGCA TTCAAGACGA TTGAACAATG TGCACACAGG AGGATTGGGA 
GGTTTCACAG TAATCTGTTT GGTTTATTCT TTTTTAAATA TGCATCCGCG AATAAAATCA 
AACGATATCG ACGTTCTAGA TAACCTGGGT GTTCTACTGA TTGACTTCTT CGAACTTTAC 
GGAAAAAATT TTGGTTATGA TGACGTTGCT ATAAGCATAT CTGATGGTTA CGCCTCTTAT 
ATTCCTAAAT CGTGTTGGAG AACTTTGGAA CCTTCAAGGA GTAAATTCTC GCTAGCTATT 
CAAGATCCTG GTGATCCTAA CAATAATATA AGCAGAGGCT CCTTTAATAT GAAGGATATA 
AAAAAGGCCT TTGCTGGTGC CTTCGAACTG TTGGTCAATA AATGCTGGGA ATTGAATTCT 
GCTACATTCA AAGACCGCGT TGGAAAAAGT ATACTCGGAA ACGTGATCAA ATACCGTGGG 
CAAAAGAGAG ATTTCAACGA CGAAAGAGAT TTAGTGCAGA ATAAAGCCAT CATTGAAAAC 
GAGCGCTACC ACAAGAGGCG CACGAGGATA GTTCAAGAAG ATTTATTTAT AAACGACACC 
GAAGACTTGC CAGTAGAGGA GATCTACAAA CTTGACGAAC CTGCAAAGAA GAAACAGAAG 
GCAAAAAAGG ATAAGAGAGA AGGGGAAATC AAAAAGAGTG CCATTCCATC TCCACCTCCG 
GATTTTGGAG TGAGCAGAAG CAAATTGAAA CGCAAAGTGA AAAAAACTGA TCAGGGCTCT 
CTCCTACACC AAAACAACCT CAGCATAGAT GACTTGATGG GACTTTCTGA GAATGATCAA 
GAATCCGATC AAGATCAAAA AGGAAGAGAT ACCCCTTCGG GACAAGATGA GAAATCACCA 
CTGGAAACTA AGACAGTAGA TGCTCAAACG AGAAGGGACT ACTGGCTCTC TAAAGGCCAG 
GCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TRF5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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