GAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GAS1
Entrez Gene ID	855355
Full Name	1,3-beta-glucanosyltransferase GAS1
Synonyms	CWH52,GGP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182818.1	NP_014038.1	1,3-beta-glucanosyltransferase GAS1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GAS1	NP_014038.1
Magnaporthe oryzae (rice blast fungus)	MGG_08370	XP_003715690.1
Kluyveromyces lactis	KLLA0C14091g	XP_452830.1
Eremothecium gossypii	AGOS_AGL352W	NP_986315.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ssays of heterologously expressed transglycosylases ScGas1, ScCrh1, and ScCrh2 from the yeast Saccharomyces cerevisiae, CaPhr1 and CaPhr2 from Candida albicans, and of a plant xyloglucan endotransglycosylase (XET) are demonstrated.
Title: Two Variants of a High-Throughput Fluorescent Microplate Assay of Polysaccharide Endotransglycosylases.

The results suggest that the dysfunction of Gas1 plays a positive role in the maintenance of rDNA integrity by decreasing PKA activity and inducing the accumulation of Msn2/4 in the nucleus.
Title: The ?-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae.

Analysis uncovers previously unsuspected functions for both Gas1 and Sas3 in DNA damage response and cell cycle regulation.
Title: Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiae.

Gas1p functions in cell wall formation, and is the primary glucan-remodeling enzyme required in vegetative growth and during reentry into the proliferative state.
Title: Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae.

Results suggest that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar, and reveal a new mechanism of protein immobilization at specific sites of the cell envelope.
Title: Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.

The following GAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04300	NM_001182818.1 Latest version!	Saccharomyces cerevisiae S288C 1,3-beta-glucanosyltransferase GAS1 (GAS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAS1 ( NM_001182818.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014038.1 Saccharomyces cerevisiae S288C 1,3-beta-glucanosyltransferase GAS1 (GAS1), partial mRNA.

Price & Availability↑

CloneID	OSi04300
Clone ID Related Accession (Same CDS sequence)	NM_001182818.1
Accession Version	NM_001182818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1,3-beta-glucanosyltransferase GAS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTGTTTA AATCCCTTTC AAAGTTAGCA ACCGCTGCTG CTTTTTTTGC TGGCGTCGCA 
ACTGCGGACG ATGTTCCAGC GATTGAAGTT GTTGGTAATA AGTTTTTCTA CTCCAACAAC 
GGTAGTCAGT TCTACATAAG AGGTGTTGCT TATCAGGCTG ATACCGCTAA TGAAACTAGC 
GGATCTACTG TCAACGATCC TTTGGCCAAT TATGAGAGTT GTTCCAGAGA TATTCCATAC 
CTCAAAAAAT TGAACACAAA TGTTATCCGT GTCTACGCTA TCAATACCAC TCTAGATCAC 
TCCGAATGTA TGAAGGCTTT GAATGATGCT GACATCTATG TCATCGCTGA TTTAGCAGCT 
CCAGCCACCT CTATCAATAG AGACGATCCA ACTTGGACTG TTGACTTGTT CAACAGCTAC 
AAAACCGTTG TTGACACTTT TGCTAATTAC ACCAACGTTT TGGGTTTCTT CGCCGGTAAT 
GAAGTTACTA ACAATTACAC CAACACAGAT GCATCTGCTT TCGTGAAGGC AGCTATTAGA 
GACGTCAGAC AATACATCAG CGACAAGAAC TACAGAAAAA TTCCAGTTGG CTACTCTTCC 
AATGATGACG AAGATACCAG AGTTAAGATG ACTGATTATT TCGCTTGTGG TGATGATGAT 
GTTAAGGCTG ATTTTTACGG TATTAATATG TATGAATGGT GTGGTAAATC TGACTTCAAA 
ACTTCTGGTT ATGCTGATAG AACTGCAGAA TTCAAAAACT TATCTATTCC TGTTTTCTTC 
TCTGAATACG GTTGTAACGA AGTAACACCA AGACTATTTA CTGAGGTTGA AGCCTTGTAC 
GGTTCTAATA TGACAGATGT CTGGTCTGGT GGTATCGTAT ACATGTACTT CGAAGAGACT 
AACAAATACG GTTTAGTTAG CATCGATGGT AATGATGTTA AAACTTTGGA TGACTTCAAC 
AACTATTCTT CTGAAATCAA CAAAATATCA CCAACTTCCG CCAACACAAA GTCTTACAGT 
GCAACAACAA GTGATGTTGC TTGTCCAGCT ACTGGTAAGT ACTGGTCCGC TGCAACAGAA 
TTACCACCAA CTCCAAACGG AGGCTTGTGT TCATGTATGA ATGCAGCCAA TAGTTGTGTT 
GTTTCCGATG ACGTTGATTC TGATGATTAC GAAACCTTAT TTAACTGGAT CTGTAATGAA 
GTCGACTGTA GCGGTATTTC AGCAAACGGT ACCGCCGGTA AGTATGGTGC TTACTCTTTC 
TGTACACCAA AGGAACAGCT ATCTTTCGTT ATGAATTTGT ACTACGAGAA GAGTGGTGGT 
AGCAAATCTG ACTGTAGCTT CAGCGGTTCT GCCACTCTAC AAACTGCCAC CACGCAAGCT 
AGTTGCTCCT CCGCTTTGAA AGAGATTGGT AGTATGGGTA CCAACTCTGC ATCAGGTAGT 
GTTGATTTGG GTTCCGGAAC TGAATCCAGT ACTGCCTCTT CTAACGCTTC GGGGTCTTCT 
TCCAAGTCTA ACTCCGGCTC TTCTGGTTCT TCCAGTTCTT CTTCTTCTTC TTCAGCTTCA 
TCTTCATCTT CTAGCAAGAA GAATGCTGCC ACCAACGTTA AAGCTAACTT AGCACAAGTG 
GTCTTTACCT CCATCATTTC CTTATCCATT GCCGCTGGTG TCGGTTTTGC TTTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014038.1
CDS	1..1680
Translation	MLFKSLSKLATAAAFFAGVATADDVPAIEVVGNKFFYSNNGSQFYIRGVAYQADTANETSGSTVNDPLANYESCSRDIPYLKKLNTNVIRVYAINTTLDHSECMKALNDADIYVIADLAAPATSINRDDPTWTVDLFNSYKTVVDTFANYTNVLGFFAGNEVTNNYTNTDASAFVKAAIRDVRQYISDKNYRKIPVGYSSNDDEDTRVKMTDYFACGDDDVKADFYGINMYEWCGKSDFKTSGYADRTAEFKNLSIPVFFSEYGCNEVTPRLFTEVEALYGSNMTDVWSGGIVYMYFEETNKYGLVSIDGNDVKTLDDFNNYSSEINKISPTSANTKSYSATTSDVACPATGKYWSAATELPPTPNGGLCSCMNAANSCVVSDDVDSDDYETLFNWICNEVDCSGISANGTAGKYGAYSFCTPKEQLSFVMNLYYEKSGGSKSDCSFSGSATLQTATTQASCSSALKEIGSMGTNSASGSVDLGSGTESSTASSNASGSSSKSNSGSSGSSSSSSSSSASSSSSSKKNAATNVKANLAQVVFTSIISLSIAAGVGFALV

Target ORF information:

RefSeq Version	NM_001182818.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1,3-beta-glucanosyltransferase GAS1 (GAS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182818.1

ORF Insert Sequence:

ATGTTGTTTA AATCCCTTTC AAAGTTAGCA ACCGCTGCTG CTTTTTTTGC TGGCGTCGCA 
ACTGCGGACG ATGTTCCAGC GATTGAAGTT GTTGGTAATA AGTTTTTCTA CTCCAACAAC 
GGTAGTCAGT TCTACATAAG AGGTGTTGCT TATCAGGCTG ATACCGCTAA TGAAACTAGC 
GGATCTACTG TCAACGATCC TTTGGCCAAT TATGAGAGTT GTTCCAGAGA TATTCCATAC 
CTCAAAAAAT TGAACACAAA TGTTATCCGT GTCTACGCTA TCAATACCAC TCTAGATCAC 
TCCGAATGTA TGAAGGCTTT GAATGATGCT GACATCTATG TCATCGCTGA TTTAGCAGCT 
CCAGCCACCT CTATCAATAG AGACGATCCA ACTTGGACTG TTGACTTGTT CAACAGCTAC 
AAAACCGTTG TTGACACTTT TGCTAATTAC ACCAACGTTT TGGGTTTCTT CGCCGGTAAT 
GAAGTTACTA ACAATTACAC CAACACAGAT GCATCTGCTT TCGTGAAGGC AGCTATTAGA 
GACGTCAGAC AATACATCAG CGACAAGAAC TACAGAAAAA TTCCAGTTGG CTACTCTTCC 
AATGATGACG AAGATACCAG AGTTAAGATG ACTGATTATT TCGCTTGTGG TGATGATGAT 
GTTAAGGCTG ATTTTTACGG TATTAATATG TATGAATGGT GTGGTAAATC TGACTTCAAA 
ACTTCTGGTT ATGCTGATAG AACTGCAGAA TTCAAAAACT TATCTATTCC TGTTTTCTTC 
TCTGAATACG GTTGTAACGA AGTAACACCA AGACTATTTA CTGAGGTTGA AGCCTTGTAC 
GGTTCTAATA TGACAGATGT CTGGTCTGGT GGTATCGTAT ACATGTACTT CGAAGAGACT 
AACAAATACG GTTTAGTTAG CATCGATGGT AATGATGTTA AAACTTTGGA TGACTTCAAC 
AACTATTCTT CTGAAATCAA CAAAATATCA CCAACTTCCG CCAACACAAA GTCTTACAGT 
GCAACAACAA GTGATGTTGC TTGTCCAGCT ACTGGTAAGT ACTGGTCCGC TGCAACAGAA 
TTACCACCAA CTCCAAACGG AGGCTTGTGT TCATGTATGA ATGCAGCCAA TAGTTGTGTT 
GTTTCCGATG ACGTTGATTC TGATGATTAC GAAACCTTAT TTAACTGGAT CTGTAATGAA 
GTCGACTGTA GCGGTATTTC AGCAAACGGT ACCGCCGGTA AGTATGGTGC TTACTCTTTC 
TGTACACCAA AGGAACAGCT ATCTTTCGTT ATGAATTTGT ACTACGAGAA GAGTGGTGGT 
AGCAAATCTG ACTGTAGCTT CAGCGGTTCT GCCACTCTAC AAACTGCCAC CACGCAAGCT 
AGTTGCTCCT CCGCTTTGAA AGAGATTGGT AGTATGGGTA CCAACTCTGC ATCAGGTAGT 
GTTGATTTGG GTTCCGGAAC TGAATCCAGT ACTGCCTCTT CTAACGCTTC GGGGTCTTCT 
TCCAAGTCTA ACTCCGGCTC TTCTGGTTCT TCCAGTTCTT CTTCTTCTTC TTCAGCTTCA 
TCTTCATCTT CTAGCAAGAA GAATGCTGCC ACCAACGTTA AAGCTAACTT AGCACAAGTG 
GTCTTTACCT CCATCATTTC CTTATCCATT GCCGCTGGTG TCGGTTTTGC TTTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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