HSH155 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HSH155
Entrez Gene ID	855332
Full Name	U2 snRNP complex subunit HSH155
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182795.1	NP_014015.1	U2 snRNP complex subunit HSH155

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09537g	XP_451417.1
Saccharomyces cerevisiae (baker's yeast)	HSH155	NP_014015.1
Eremothecium gossypii	AGOS_ADR334W	NP_984430.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.
Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.

different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.

show here that hsh155 mutant alleles in Saccharomyces cerevisiae, counterparts of SF3B1 mutations frequently found in cancers, specifically change splicing of suboptimal BS pre-mRNA substrates
Title: SF3B1/Hsh155 HEAT motif mutations affect interaction with the spliceosomal ATPase Prp5, resulting in altered branch site selectivity in pre-mRNA splicing.

The following HSH155 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSH155 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04281	NM_001182795.1 Latest version!	Saccharomyces cerevisiae S288C U2 snRNP complex subunit HSH155 (HSH155), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSH155 ( NM_001182795.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014015.1 Saccharomyces cerevisiae S288C U2 snRNP complex subunit HSH155 (HSH155), partial mRNA.

Price & Availability↑

CloneID	OSi04281
Clone ID Related Accession (Same CDS sequence)	NM_001182795.1
Accession Version	NM_001182795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	U2 snRNP complex subunit HSH155
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTCATC CGATTCAATT TGTAAACGCT AATAATAGCG ATAAGAGCCA CCAATTAGGC 
GGTCAATATT CTATTCCTCA GGATTTGAGG GAAAACCTGC AGAAAGAAGC TGCACGTATT 
GGTGAGAACG AAAAAGATGT GCTACAGGAA AAAATGGAAA CTAGGACAGT GCAAAATCGA 
GAGGATTCAT ACCACAAAAG AAGGTTTGAT ATGAAGTTTG AGCCGGATTC CGATACACAA 
ACTGTTACGT CTTCAGAAAA TACCCAAGAC GCTGTCGTGC CAAGGAAAAG AAAATCTCGT 
TGGGATGTAA AAGGGTACGA GCCACCTGAT GAATCTTCAA CAGCGGTCAA AGAAAACTCT 
GATAGTGCTT TGGTTAATGT AGAAGGAATC CATGATTTGA TGTTTTTTAA ACCTTCTGAC 
CATAAATATT TCGCCGACGT TATATCCAAA AAACCCATAG ATGAGTTAAA TAAGGATGAA 
AAGAAAGAAA GAACCCTGTC GATGTTACTT CTGAAAATAA AAAATGGCAA TACAGCGAGT 
AGGAGGACTT CTATGCGGAT TTTAACCGAT AAAGCTGTTA CATTTGGTCC AGAAATGATA 
TTTAACCGCT TATTGCCCAT ATTACTAGAC AGAAGTTTGG AAGACCAGGA AAGACATCTA 
ATGATCAAGA CCATAGATCG CGTCCTTTAC CAGCTAGGAG ATTTAACAAA ACCTTACGTT 
CACAAAATAC TTGTAGTAGC TGCCCCATTG CTAATAGATG AAGATCCAAT GGTTCGTTCA 
ACAGGCCAGG AGATAATCAC CAATCTGTCT ACAGTCGCTG GCCTAAAAAC CATTCTAACA 
GTAATGAGAC CTGATATTGA GAATGAGGAT GAGTATGTTA GAAATGTAAC TTCAAGAGCG 
GCGGCTGTGG TGGCAAAAGC CCTTGGTGTT AATCAATTGC TTCCTTTCAT CAATGCAGCA 
TGCCACTCAC GGAAGTCTTG GAAAGCAAGG CATACTGGCA TAAAAATTGT GCAGCAAATA 
GGTATACTTT TGGGAATAGG TGTCTTGAAT CATCTTACTG GGCTGATGAG TTGTATCAAA 
GACTGCCTAA TGGATGATCA CGTTCCGGTG CGAATTGTTA CAGCACATAC TTTATCAACA 
CTAGCAGAAA ATTCTTATCC ATATGGTATC GAAGTTTTCA ACGTTGTACT GGAGCCATTA 
TGGAAGGGTA TAAGAAGTCA TCGTGGTAAA GTGCTATCTT CTTTTTTAAA AGCTGTTGGA 
TCTATGATCC CATTGATGGA CCCAGAGTAT GCCGGTTATT ATACCACAGA AGCAATGAGA 
ATAATTAGAC GTGAATTCGA CTCTCCCGAT GATGAAATGA AAAAGACCAT TCTTTTAGTA 
TTACAAAAAT GTAGCGCTGT AGAATCCATA ACGCCAAAAT TTCTGAGAGA AGAAATCGCG 
CCAGAATTCT TTCAAAAATT TTGGGTCAGA CGAGTGGCAT TGGATAGACC ATTAAACAAA 
GTTGTTACAT ATACTACTGT TACATTAGCA AAGAAATTAG GTTGTTCTTA TACCATTGAT 
AAATTATTAA CACCATTGAG AGATGAAGCT GAGCCGTTCA GAACAATGGC TGTCCATGCT 
GTTACTAGAA CAGTAAACTT ATTAGGGACA GCAGATTTAG ATGAGAGATT AGAAACGAGG 
CTTATTGATG CGCTCTTGAT AGCATTTCAA GAACAAACAA ATAGTGATTC TATAATCTTT 
AAAGGGTTTG GGGCAGTGAC GGTGTCACTT GACATCCGTA TGAAACCGTT TTTAGCACCC 
ATTGTGAGTA CGATTTTAAA TCATTTAAAG CATAAAACTC CCTTGGTTCG TCAACATGCA 
GCAGATCTAT GTGCCATACT AATACCAGTC ATCAAGAACT GTCACGAATT CGAAATGTTG 
AATAAATTGA ACATTATATT GTATGAATCC TTAGGCGAAG TATATCCAGA GGTATTAGGT 
TCTATAATCA ACGCTATGTA CTGTATTACT AGCGTAATGG ATTTGGATAA ACTACAACCT 
CCTATTAATC AAATTTTACC AACCTTAACT CCAATTTTAA GAAACAAGCA TAGAAAAGTC 
GAAGTAAACA CTATCAAATT TGTTGGACTT ATTGGTAAAT TAGCACCTAC CTATGCACCT 
CCTAAAGAAT GGATGAGAAT ATGTTTTGAA CTATTAGAAC TTTTGAAAAG TACAAATAAA 
GAAATAAGAA GATCAGCAAA TGCCACGTTT GGATTCATTG CTGAAGCAAT TGGACCTCAC 
GATGTTCTTG TTGCGTTATT GAACAATTTA AAGGTTCAAG AACGTCAGTT ACGTGTATGC 
ACGGCTGTTG CTATCGGTAT CGTTGCTAAA GTGTGTGGTC CTTACAACGT GCTGCCAGTA 
ATCATGAATG AATACACAAC ACCAGAGACT AATGTTCAAA ACGGTGTTCT CAAGGCCATG 
TCATTCATGT TTGAGTACAT TGGTAACATG TCTAAGGATT ACATATACTT CATAACACCA 
TTATTAGAAG ATGCACTCAC CGATAGGGAT TTAGTTCATC GTCAGACAGC GTCGAACGTA 
ATAACCCACT TAGCTTTAAA CTGTTCAGGT ACAGGTCACG AAGATGCCTT CATTCATTTA 
ATGAATCTTC TAATACCCAA TATTTTTGAA ACTTCACCAC ATGCTATTAT GCGTATTTTA 
GAAGGTTTGG AGGCACTGAG TCAAGCTCTT GGCCCTGGAC TATTCATGAA CTACATATGG 
GCAGGATTGT TCCATCCAGC GAAGAATGTA AGGAAAGCAT TTTGGAGGGT GTATAATAAC 
ATGTACGTAA TGTACCAGGA CGCCATGGTA CCTTTTTACC CCGTTACACC AGACAACAAT 
GAAGAATATA TAGAAGAACT GGATTTAGTT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014015.1
CDS	1..2916
Translation	MSHPIQFVNANNSDKSHQLGGQYSIPQDLRENLQKEAARIGENEKDVLQEKMETRTVQNREDSYHKRRFDMKFEPDSDTQTVTSSENTQDAVVPRKRKSRWDVKGYEPPDESSTAVKENSDSALVNVEGIHDLMFFKPSDHKYFADVISKKPIDELNKDEKKERTLSMLLLKIKNGNTASRRTSMRILTDKAVTFGPEMIFNRLLPILLDRSLEDQERHLMIKTIDRVLYQLGDLTKPYVHKILVVAAPLLIDEDPMVRSTGQEIITNLSTVAGLKTILTVMRPDIENEDEYVRNVTSRAAAVVAKALGVNQLLPFINAACHSRKSWKARHTGIKIVQQIGILLGIGVLNHLTGLMSCIKDCLMDDHVPVRIVTAHTLSTLAENSYPYGIEVFNVVLEPLWKGIRSHRGKVLSSFLKAVGSMIPLMDPEYAGYYTTEAMRIIRREFDSPDDEMKKTILLVLQKCSAVESITPKFLREEIAPEFFQKFWVRRVALDRPLNKVVTYTTVTLAKKLGCSYTIDKLLTPLRDEAEPFRTMAVHAVTRTVNLLGTADLDERLETRLIDALLIAFQEQTNSDSIIFKGFGAVTVSLDIRMKPFLAPIVSTILNHLKHKTPLVRQHAADLCAILIPVIKNCHEFEMLNKLNIILYESLGEVYPEVLGSIINAMYCITSVMDLDKLQPPINQILPTLTPILRNKHRKVEVNTIKFVGLIGKLAPTYAPPKEWMRICFELLELLKSTNKEIRRSANATFGFIAEAIGPHDVLVALLNNLKVQERQLRVCTAVAIGIVAKVCGPYNVLPVIMNEYTTPETNVQNGVLKAMSFMFEYIGNMSKDYIYFITPLLEDALTDRDLVHRQTASNVITHLALNCSGTGHEDAFIHLMNLLIPNIFETSPHAIMRILEGLEALSQALGPGLFMNYIWAGLFHPAKNVRKAFWRVYNNMYVMYQDAMVPFYPVTPDNNEEYIEELDLVL

Target ORF information:

RefSeq Version	NM_001182795.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C U2 snRNP complex subunit HSH155 (HSH155), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182795.1

ORF Insert Sequence:

ATGAGTCATC CGATTCAATT TGTAAACGCT AATAATAGCG ATAAGAGCCA CCAATTAGGC 
GGTCAATATT CTATTCCTCA GGATTTGAGG GAAAACCTGC AGAAAGAAGC TGCACGTATT 
GGTGAGAACG AAAAAGATGT GCTACAGGAA AAAATGGAAA CTAGGACAGT GCAAAATCGA 
GAGGATTCAT ACCACAAAAG AAGGTTTGAT ATGAAGTTTG AGCCGGATTC CGATACACAA 
ACTGTTACGT CTTCAGAAAA TACCCAAGAC GCTGTCGTGC CAAGGAAAAG AAAATCTCGT 
TGGGATGTAA AAGGGTACGA GCCACCTGAT GAATCTTCAA CAGCGGTCAA AGAAAACTCT 
GATAGTGCTT TGGTTAATGT AGAAGGAATC CATGATTTGA TGTTTTTTAA ACCTTCTGAC 
CATAAATATT TCGCCGACGT TATATCCAAA AAACCCATAG ATGAGTTAAA TAAGGATGAA 
AAGAAAGAAA GAACCCTGTC GATGTTACTT CTGAAAATAA AAAATGGCAA TACAGCGAGT 
AGGAGGACTT CTATGCGGAT TTTAACCGAT AAAGCTGTTA CATTTGGTCC AGAAATGATA 
TTTAACCGCT TATTGCCCAT ATTACTAGAC AGAAGTTTGG AAGACCAGGA AAGACATCTA 
ATGATCAAGA CCATAGATCG CGTCCTTTAC CAGCTAGGAG ATTTAACAAA ACCTTACGTT 
CACAAAATAC TTGTAGTAGC TGCCCCATTG CTAATAGATG AAGATCCAAT GGTTCGTTCA 
ACAGGCCAGG AGATAATCAC CAATCTGTCT ACAGTCGCTG GCCTAAAAAC CATTCTAACA 
GTAATGAGAC CTGATATTGA GAATGAGGAT GAGTATGTTA GAAATGTAAC TTCAAGAGCG 
GCGGCTGTGG TGGCAAAAGC CCTTGGTGTT AATCAATTGC TTCCTTTCAT CAATGCAGCA 
TGCCACTCAC GGAAGTCTTG GAAAGCAAGG CATACTGGCA TAAAAATTGT GCAGCAAATA 
GGTATACTTT TGGGAATAGG TGTCTTGAAT CATCTTACTG GGCTGATGAG TTGTATCAAA 
GACTGCCTAA TGGATGATCA CGTTCCGGTG CGAATTGTTA CAGCACATAC TTTATCAACA 
CTAGCAGAAA ATTCTTATCC ATATGGTATC GAAGTTTTCA ACGTTGTACT GGAGCCATTA 
TGGAAGGGTA TAAGAAGTCA TCGTGGTAAA GTGCTATCTT CTTTTTTAAA AGCTGTTGGA 
TCTATGATCC CATTGATGGA CCCAGAGTAT GCCGGTTATT ATACCACAGA AGCAATGAGA 
ATAATTAGAC GTGAATTCGA CTCTCCCGAT GATGAAATGA AAAAGACCAT TCTTTTAGTA 
TTACAAAAAT GTAGCGCTGT AGAATCCATA ACGCCAAAAT TTCTGAGAGA AGAAATCGCG 
CCAGAATTCT TTCAAAAATT TTGGGTCAGA CGAGTGGCAT TGGATAGACC ATTAAACAAA 
GTTGTTACAT ATACTACTGT TACATTAGCA AAGAAATTAG GTTGTTCTTA TACCATTGAT 
AAATTATTAA CACCATTGAG AGATGAAGCT GAGCCGTTCA GAACAATGGC TGTCCATGCT 
GTTACTAGAA CAGTAAACTT ATTAGGGACA GCAGATTTAG ATGAGAGATT AGAAACGAGG 
CTTATTGATG CGCTCTTGAT AGCATTTCAA GAACAAACAA ATAGTGATTC TATAATCTTT 
AAAGGGTTTG GGGCAGTGAC GGTGTCACTT GACATCCGTA TGAAACCGTT TTTAGCACCC 
ATTGTGAGTA CGATTTTAAA TCATTTAAAG CATAAAACTC CCTTGGTTCG TCAACATGCA 
GCAGATCTAT GTGCCATACT AATACCAGTC ATCAAGAACT GTCACGAATT CGAAATGTTG 
AATAAATTGA ACATTATATT GTATGAATCC TTAGGCGAAG TATATCCAGA GGTATTAGGT 
TCTATAATCA ACGCTATGTA CTGTATTACT AGCGTAATGG ATTTGGATAA ACTACAACCT 
CCTATTAATC AAATTTTACC AACCTTAACT CCAATTTTAA GAAACAAGCA TAGAAAAGTC 
GAAGTAAACA CTATCAAATT TGTTGGACTT ATTGGTAAAT TAGCACCTAC CTATGCACCT 
CCTAAAGAAT GGATGAGAAT ATGTTTTGAA CTATTAGAAC TTTTGAAAAG TACAAATAAA 
GAAATAAGAA GATCAGCAAA TGCCACGTTT GGATTCATTG CTGAAGCAAT TGGACCTCAC 
GATGTTCTTG TTGCGTTATT GAACAATTTA AAGGTTCAAG AACGTCAGTT ACGTGTATGC 
ACGGCTGTTG CTATCGGTAT CGTTGCTAAA GTGTGTGGTC CTTACAACGT GCTGCCAGTA 
ATCATGAATG AATACACAAC ACCAGAGACT AATGTTCAAA ACGGTGTTCT CAAGGCCATG 
TCATTCATGT TTGAGTACAT TGGTAACATG TCTAAGGATT ACATATACTT CATAACACCA 
TTATTAGAAG ATGCACTCAC CGATAGGGAT TTAGTTCATC GTCAGACAGC GTCGAACGTA 
ATAACCCACT TAGCTTTAAA CTGTTCAGGT ACAGGTCACG AAGATGCCTT CATTCATTTA 
ATGAATCTTC TAATACCCAA TATTTTTGAA ACTTCACCAC ATGCTATTAT GCGTATTTTA 
GAAGGTTTGG AGGCACTGAG TCAAGCTCTT GGCCCTGGAC TATTCATGAA CTACATATGG 
GCAGGATTGT TCCATCCAGC GAAGAATGTA AGGAAAGCAT TTTGGAGGGT GTATAATAAC 
ATGTACGTAA TGTACCAGGA CGCCATGGTA CCTTTTTACC CCGTTACACC AGACAACAAT 
GAAGAATATA TAGAAGAACT GGATTTAGTT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HSH155 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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