MTF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA.
Title: The Yeast Mitochondrial RNA Polymerase and Transcription Factor Complex Catalyzes Efficient Priming of DNA Synthesis on Single-stranded DNA.

The study examined a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis.
Title: Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41.

This study identifies the importance of the +1+2 AA promoter sequence interactions with Rpo41-Mtf1 in dictating pre-transcribing complex formation, which regulates the binding of initiating NTPs and the catalytic efficiency of transcription initiation.
Title: Interactions of the yeast mitochondrial RNA polymerase with the +1 and +2 promoter bases dictate transcription initiation efficiency.

While Rpo41 induces a slight bend upon binding both the DNAs, addition of the Mtf1 results in severe bending of the promoter and unbending of the non-promoter DNA
Title: Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase.

Data show that he promoter DNA is sandwiched between Mtf1 and Rpo41 in the structural model.
Title: Mitochondrial transcription factor Mtf1 traps the unwound non-template strand to facilitate open complex formation.