SSM4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SSM4
Entrez Gene ID	854781
Full Name	E3 ubiquitin-protein ligase SSM4
Synonyms	DOA10,KIS3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179380.3	NP_012234.3	E3 ubiquitin-protein ligase SSM4

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADL090W	NP_984006.1
Saccharomyces cerevisiae (baker's yeast)	SSM4	NP_012234.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sequential poly-ubiquitylation by specialized conjugating enzymes Ubc6 and Ubc7 expands the versatility of a quality control ubiquitin ligase Doa10.
Title: Sequential Poly-ubiquitylation by Specialized Conjugating Enzymes Expands the Versatility of a Quality Control Ubiquitin Ligase.

Here, the authors show that the ERAD ubiquitin ligase Doa10 controls the levels of some lipid droplet proteins.
Title: Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10.

Data suggest that human MARCH6 and Saccharomyces cerevisiae Doa10 ubiquitin ligases are functionally similar.
Title: A Conserved C-terminal Element in the Yeast Doa10 and Human MARCH6 Ubiquitin Ligases Required for Selective Substrate Degradation.

Results show Ypf1 is an Endoplasmic Reticulum-resident aspartyl intramembrane protease with a presenilin fold, and identified Doa10 and Dfm1 as its cofactors.
Title: The yeast ER-intramembrane protease Ypf1 refines nutrient sensing by regulating transporter abundance.

the TEB4-Doa10 domain regulates Doa10 association with the Ubc6 membrane anchor, thereby controlling the degradation rate of the E2.
Title: An unusual transmembrane helix in the endoplasmic reticulum ubiquitin ligase Doa10 modulates degradation of its cognate E2 enzyme.

The following SSM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03804	NM_001179380.3 Latest version!	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase SSM4 (SSM4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSM4 ( NM_001179380.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012234.3 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase SSM4 (SSM4), partial mRNA.

Price & Availability↑

CloneID	OSi03804
Clone ID Related Accession (Same CDS sequence)	NM_001179380.3
Accession Version	NM_001179380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	E3 ubiquitin-protein ligase SSM4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Jul 30, 2012 this sequence version replaced NM_001179380.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGTTG ATTCTGACGT TAATGTCTCC AGGTTAAGAG ATGAACTGCA TAAGGTGGCA 
AACGAGGAAA CAGATACCGC CACTTTCAAT GATGATGCGC CATCCGGCGC CACCTGCCGT 
ATTTGTCGTG GAGAAGCTAC TGAGGACAAT CCTCTTTTCC ATCCTTGTAA ATGCAGGGGC 
TCAATTAAAT ACATGCATGA ATCCTGTCTG TTGGAATGGG TAGCTTCAAA AAATATAGAC 
ATTTCAAAAC CAGGCGCGGA TGTTAAATGT GACATCTGTC ACTATCCCAT TCAATTCAAA 
ACGATATATG CGGAAAACAT GCCCGAAAAG ATACCTTTTT CTTTACTACT ATCCAAGAGC 
ATCTTGACAT TTTTCGAAAA AGCTCGTTTG GCTTTAACGA TTGGTTTGGC CGCTGTACTA 
TATATTATCG GTGTTCCTTT GGTATGGAAC ATGTTTGGAA AATTATACAC CATGATGCTA 
GATGGATCAT CCCCTTATCC TGGTGATTTC CTAAAGAGTT TGATTTATGG TTATGATCAG 
TCAGCAACTC CAGAATTAAC CACGAGAGCA ATTTTTTACC AACTTTTACA AAACCATTCG 
TTTACGTCTT TACAATTTAT TATGATAGTG ATACTACATA TAGCATTGTA CTTTCAATAC 
GATATGATAG TAAGAGAGGA TGTTTTCAGT AAAATGGTTT TCCACAAGAT AGGTCCAAGA 
TTATCTCCCA AGGACTTGAA ATCACGTTTG AAGGAAAGAT TCCCCATGAT GGATGATAGA 
ATGGTTGAAT ATTTAGCGAG GGAAATGAGA GCTCATGACG AAAATCGTCA AGAGCAAGGT 
CATGATAGAC TGAACATGCC AGCCGCAGCA GCAGATAATA ATAATAATGT TATTAATCCC 
AGAAACGATA ACGTTCCACC TCAGGATCCT AATGACCATC GGAATTTTGA AAACCTTCGC 
CACGTAGACG AATTAGATCA TGATGAGGCT ACGGAAGAGC ACGAGAACAA CGACTCTGAC 
AATAGTTTAC CATCAGGTGA TGATTCGTCA CGAATTTTAC CGGGGTCTTC AAGTGACAAC 
GAAGAAGATG AAGAAGCAGA GGGGCAACAG CAACAGCAGC AACCAGAAGA AGAGGCAGAT 
TACCGTGATC ATATAGAGCC CAATCCGATT GACATGTGGG CAAATCGTCG TGCGCAAAAT 
GAATTTGATG ACCTAATCGC TGCTCAACAG AACGCAATAA ACAGGCCCAA CGCTCCGGTT 
TTCATCCCAC CACCTGCCCA AAACAGAGCT GGAAATGTCG ATCAAGACGA ACAAGACTTT 
GGCGCAGCTG TTGGTGTGCC ACCAGCACAG GCTAACCCAG ACGATCAAGG ACAAGGCCCT 
CTAGTGATAA ATCTCAAATT GAAATTGTTG AATGTCATTG CTTATTTCAT AATTGCCGTA 
GTTTTCACCG CAATATACCT GGCAATTTCC TATTTGTTTC CAACTTTTAT TGGGTTCGGT 
TTGTTGAAAA TTTATTTTGG TATATTTAAG GTTATTTTGC GTGGGTTGTG CCATCTTTAC 
TACTTGTCTG GCGCTCATAT AGCTTACAAT GGGCTGACGA AACTGGTCCC TAAAGTAGAT 
GTCGCGATGA GTTGGATTTC AGACCACTTA ATTCATGACA TCATTTATCT TTACAACGGC 
TACACTGAAA ACACCATGAA GCACTCAATT TTCATCAGAG CTTTACCTGC TTTAACGACG 
TATCTAACCT CAGTCAGTAT CGTTTGTGCT TCATCCAATC TTGTCTCGAG GGGTTATGGC 
CGTGAGAACG GAATGAGCAA CCCTACTAGA AGGCTGATTT TCCAGATTTT ATTTGCACTC 
AAATGCACTT TCAAGGTGTT CACTTTATTT TTCATCGAAT TGGCTGGATT CCCCATTCTA 
GCTGGTGTAA TGCTAGATTT CTCATTATTT TGCCCAATCC TGGCATCCAA TAGTCGGATG 
CTTTGGGTTC CTTCAATATG CGCAATATGG CCTCCATTTT CCCTTTTCGT ATACTGGACG 
ATTGGTACTC TCTACATGTA TTGGTTTGCC AAATATATCG GAATGATTAG GAAAAATATC 
ATTAGACCAG GTGTTCTATT CTTTATCAGA TCTCCAGAAG ATCCTAATAT TAAGATCTTG 
CATGATAGTT TAATTCATCC GATGAGTATT CAATTATCAA GGCTTTGCTT ATCCATGTTT 
ATTTATGCGA TTTTTATTGT CTTAGGTTTT GGCTTCCATA CTAGAATTTT TTTCCCATTC 
ATGCTCAAAT CGAACTTATT ATCCGTTCCT GAAGCTTATA AACCAACATC AATTATTTCA 
TGGAAGTTCA ATACAATTTT GCTAACTTTA TATTTTACGA AGAGGATCCT GGAGTCTAGC 
TCTTACGTTA AACCCTTATT GGAAAGGTAT TGGAAAACAA TTTTTAAGTT ATGTTCAAGA 
AAATTACGTT TATCATCCTT TATCTTAGGT AAAGATACCC CAACAGAGAG AGGCCATATT 
GTTTACCGCA ATTTGTTTTA CAAGTACATT GCTGCCAAGA ATGCAGAATG GTCAAACCAA 
GAACTTTTCA CTAAACCAAA GACTCTAGAA CAAGCAGAAG AATTGTTTGG GCAAGTTCGT 
GACGTTCATG CTTATTTTGT CCCCGATGGC GTTTTAATGC GTGTTCCTTC TTCTGATATA 
GTATCCAGAA ATTATGTACA AACAATGTTT GTCCCCGTTA CAAAGGATGA TAAGCTGTTG 
AAACCATTAG ATTTGGAAAG AATTAAAGAG AGAAACAAAC GTGCTGCTGG TGAATTTGGT 
TATTTAGACG AACAAAACAC GGAATATGAT CAATATTATA TCGTTTACGT CCCTCCAGAT 
TTCCGTTTAC GCTATATGAC ATTGCTTGGC CTGGTTTGGT TATTTGCTTC CATTTTAATG 
CTTGGTGTTA CATTTATTAG TCAAGCTTTA ATAAACTTTG TTTGTAGTTT CGGCTTTCTT 
CCAGTCGTAA AGCTATTGTT AGGTGAAAGA AATAAGGTCT ATGTTGCATG GAAGGAACTC 
TCTGACATTA GCTACTCCTA TTTGAACATT TATTATGTCT GTGTCGGATC TGTTTGTTTA 
TCCAAGATTG CTAAGGATAT TCTTCATTTT ACAGAAGGCC AAAACACTCT CGATGAACAT 
GCCGTTGACG AAAACGAAGT AGAGGAAGTG GAACACGATA TACCAGAGAG AGATATCAAC 
AATGCCCCTG TTAATAATAT CAATAATGTG GAAGAGGGTC AAGGAATTTT CATGGCCATC 
TTTAACAGTA TATTTGATAG TATGTTGGTG AAGTATAACC TGATGGTGTT TATAGCTATC 
ATGATTGCGG TTATTAGAAC TATGGTTTCG TGGGTCGTCT TAACCGATGG TATACTAGCC 
TGTTACAATT ATCTCACCAT CAGAGTGTTT GGCAATTCTT CATACACTAT TGGTAATTCC 
AAATGGTTTA AATATGACGA GAGTTTGTTA TTCGTCGTTT GGATCATTTC GTCAATGGTC 
AATTTCGGAA CCGGATATAA GTCATTGAAA CTATTTTTCA GAAACAGAAA TACTAGTAAA 
TTGAACTTCT TGAAAACAAT GGCTTTGGAA CTGTTTAAAC AAGGTTTCCT GCACATGGTA 
ATTTACGTCT TGCCGATTAT TATTTTATCC TTAGTATTTT TACGTGATGT TTCTACAAAG 
CAGATTATCG ATATTAGCCA TGGATCCAGG AGTTTCACTT TATCGCTTAA CGAATCATTC 
CCAACATGGA CAAGGATGCA AGATATTTAT TTCGGACTAC TGATAGCATT AGAGTCATTT 
ACGTTCTTTT TCCAAGCAAC TGTGCTGTTC ATTCAATGGT TCAAAAGTAC TGTACAGAAT 
GTAAAAGACG AGGTTTACAC TAAGGGTAGA GCTTTAGAAA ATTTACCAGA TGAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012234.3
CDS	1..3960
Translation	MDVDSDVNVSRLRDELHKVANEETDTATFNDDAPSGATCRICRGEATEDNPLFHPCKCRGSIKYMHESCLLEWVASKNIDISKPGADVKCDICHYPIQFKTIYAENMPEKIPFSLLLSKSILTFFEKARLALTIGLAAVLYIIGVPLVWNMFGKLYTMMLDGSSPYPGDFLKSLIYGYDQSATPELTTRAIFYQLLQNHSFTSLQFIMIVILHIALYFQYDMIVREDVFSKMVFHKIGPRLSPKDLKSRLKERFPMMDDRMVEYLAREMRAHDENRQEQGHDRLNMPAAAADNNNNVINPRNDNVPPQDPNDHRNFENLRHVDELDHDEATEEHENNDSDNSLPSGDDSSRILPGSSSDNEEDEEAEGQQQQQQPEEEADYRDHIEPNPIDMWANRRAQNEFDDLIAAQQNAINRPNAPVFIPPPAQNRAGNVDQDEQDFGAAVGVPPAQANPDDQGQGPLVINLKLKLLNVIAYFIIAVVFTAIYLAISYLFPTFIGFGLLKIYFGIFKVILRGLCHLYYLSGAHIAYNGLTKLVPKVDVAMSWISDHLIHDIIYLYNGYTENTMKHSIFIRALPALTTYLTSVSIVCASSNLVSRGYGRENGMSNPTRRLIFQILFALKCTFKVFTLFFIELAGFPILAGVMLDFSLFCPILASNSRMLWVPSICAIWPPFSLFVYWTIGTLYMYWFAKYIGMIRKNIIRPGVLFFIRSPEDPNIKILHDSLIHPMSIQLSRLCLSMFIYAIFIVLGFGFHTRIFFPFMLKSNLLSVPEAYKPTSIISWKFNTILLTLYFTKRILESSSYVKPLLERYWKTIFKLCSRKLRLSSFILGKDTPTERGHIVYRNLFYKYIAAKNAEWSNQELFTKPKTLEQAEELFGQVRDVHAYFVPDGVLMRVPSSDIVSRNYVQTMFVPVTKDDKLLKPLDLERIKERNKRAAGEFGYLDEQNTEYDQYYIVYVPPDFRLRYMTLLGLVWLFASILMLGVTFISQALINFVCSFGFLPVVKLLLGERNKVYVAWKELSDISYSYLNIYYVCVGSVCLSKIAKDILHFTEGQNTLDEHAVDENEVEEVEHDIPERDINNAPVNNINNVEEGQGIFMAIFNSIFDSMLVKYNLMVFIAIMIAVIRTMVSWVVLTDGILACYNYLTIRVFGNSSYTIGNSKWFKYDESLLFVVWIISSMVNFGTGYKSLKLFFRNRNTSKLNFLKTMALELFKQGFLHMVIYVLPIIILSLVFLRDVSTKQIIDISHGSRSFTLSLNESFPTWTRMQDIYFGLLIALESFTFFFQATVLFIQWFKSTVQNVKDEVYTKGRALENLPDES

Target ORF information:

RefSeq Version	NM_001179380.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase SSM4 (SSM4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179380.3

ORF Insert Sequence:

ATGGATGTTG ATTCTGACGT TAATGTCTCC AGGTTAAGAG ATGAACTGCA TAAGGTGGCA 
AACGAGGAAA CAGATACCGC CACTTTCAAT GATGATGCGC CATCCGGCGC CACCTGCCGT 
ATTTGTCGTG GAGAAGCTAC TGAGGACAAT CCTCTTTTCC ATCCTTGTAA ATGCAGGGGC 
TCAATTAAAT ACATGCATGA ATCCTGTCTG TTGGAATGGG TAGCTTCAAA AAATATAGAC 
ATTTCAAAAC CAGGCGCGGA TGTTAAATGT GACATCTGTC ACTATCCCAT TCAATTCAAA 
ACGATATATG CGGAAAACAT GCCCGAAAAG ATACCTTTTT CTTTACTACT ATCCAAGAGC 
ATCTTGACAT TTTTCGAAAA AGCTCGTTTG GCTTTAACGA TTGGTTTGGC CGCTGTACTA 
TATATTATCG GTGTTCCTTT GGTATGGAAC ATGTTTGGAA AATTATACAC CATGATGCTA 
GATGGATCAT CCCCTTATCC TGGTGATTTC CTAAAGAGTT TGATTTATGG TTATGATCAG 
TCAGCAACTC CAGAATTAAC CACGAGAGCA ATTTTTTACC AACTTTTACA AAACCATTCG 
TTTACGTCTT TACAATTTAT TATGATAGTG ATACTACATA TAGCATTGTA CTTTCAATAC 
GATATGATAG TAAGAGAGGA TGTTTTCAGT AAAATGGTTT TCCACAAGAT AGGTCCAAGA 
TTATCTCCCA AGGACTTGAA ATCACGTTTG AAGGAAAGAT TCCCCATGAT GGATGATAGA 
ATGGTTGAAT ATTTAGCGAG GGAAATGAGA GCTCATGACG AAAATCGTCA AGAGCAAGGT 
CATGATAGAC TGAACATGCC AGCCGCAGCA GCAGATAATA ATAATAATGT TATTAATCCC 
AGAAACGATA ACGTTCCACC TCAGGATCCT AATGACCATC GGAATTTTGA AAACCTTCGC 
CACGTAGACG AATTAGATCA TGATGAGGCT ACGGAAGAGC ACGAGAACAA CGACTCTGAC 
AATAGTTTAC CATCAGGTGA TGATTCGTCA CGAATTTTAC CGGGGTCTTC AAGTGACAAC 
GAAGAAGATG AAGAAGCAGA GGGGCAACAG CAACAGCAGC AACCAGAAGA AGAGGCAGAT 
TACCGTGATC ATATAGAGCC CAATCCGATT GACATGTGGG CAAATCGTCG TGCGCAAAAT 
GAATTTGATG ACCTAATCGC TGCTCAACAG AACGCAATAA ACAGGCCCAA CGCTCCGGTT 
TTCATCCCAC CACCTGCCCA AAACAGAGCT GGAAATGTCG ATCAAGACGA ACAAGACTTT 
GGCGCAGCTG TTGGTGTGCC ACCAGCACAG GCTAACCCAG ACGATCAAGG ACAAGGCCCT 
CTAGTGATAA ATCTCAAATT GAAATTGTTG AATGTCATTG CTTATTTCAT AATTGCCGTA 
GTTTTCACCG CAATATACCT GGCAATTTCC TATTTGTTTC CAACTTTTAT TGGGTTCGGT 
TTGTTGAAAA TTTATTTTGG TATATTTAAG GTTATTTTGC GTGGGTTGTG CCATCTTTAC 
TACTTGTCTG GCGCTCATAT AGCTTACAAT GGGCTGACGA AACTGGTCCC TAAAGTAGAT 
GTCGCGATGA GTTGGATTTC AGACCACTTA ATTCATGACA TCATTTATCT TTACAACGGC 
TACACTGAAA ACACCATGAA GCACTCAATT TTCATCAGAG CTTTACCTGC TTTAACGACG 
TATCTAACCT CAGTCAGTAT CGTTTGTGCT TCATCCAATC TTGTCTCGAG GGGTTATGGC 
CGTGAGAACG GAATGAGCAA CCCTACTAGA AGGCTGATTT TCCAGATTTT ATTTGCACTC 
AAATGCACTT TCAAGGTGTT CACTTTATTT TTCATCGAAT TGGCTGGATT CCCCATTCTA 
GCTGGTGTAA TGCTAGATTT CTCATTATTT TGCCCAATCC TGGCATCCAA TAGTCGGATG 
CTTTGGGTTC CTTCAATATG CGCAATATGG CCTCCATTTT CCCTTTTCGT ATACTGGACG 
ATTGGTACTC TCTACATGTA TTGGTTTGCC AAATATATCG GAATGATTAG GAAAAATATC 
ATTAGACCAG GTGTTCTATT CTTTATCAGA TCTCCAGAAG ATCCTAATAT TAAGATCTTG 
CATGATAGTT TAATTCATCC GATGAGTATT CAATTATCAA GGCTTTGCTT ATCCATGTTT 
ATTTATGCGA TTTTTATTGT CTTAGGTTTT GGCTTCCATA CTAGAATTTT TTTCCCATTC 
ATGCTCAAAT CGAACTTATT ATCCGTTCCT GAAGCTTATA AACCAACATC AATTATTTCA 
TGGAAGTTCA ATACAATTTT GCTAACTTTA TATTTTACGA AGAGGATCCT GGAGTCTAGC 
TCTTACGTTA AACCCTTATT GGAAAGGTAT TGGAAAACAA TTTTTAAGTT ATGTTCAAGA 
AAATTACGTT TATCATCCTT TATCTTAGGT AAAGATACCC CAACAGAGAG AGGCCATATT 
GTTTACCGCA ATTTGTTTTA CAAGTACATT GCTGCCAAGA ATGCAGAATG GTCAAACCAA 
GAACTTTTCA CTAAACCAAA GACTCTAGAA CAAGCAGAAG AATTGTTTGG GCAAGTTCGT 
GACGTTCATG CTTATTTTGT CCCCGATGGC GTTTTAATGC GTGTTCCTTC TTCTGATATA 
GTATCCAGAA ATTATGTACA AACAATGTTT GTCCCCGTTA CAAAGGATGA TAAGCTGTTG 
AAACCATTAG ATTTGGAAAG AATTAAAGAG AGAAACAAAC GTGCTGCTGG TGAATTTGGT 
TATTTAGACG AACAAAACAC GGAATATGAT CAATATTATA TCGTTTACGT CCCTCCAGAT 
TTCCGTTTAC GCTATATGAC ATTGCTTGGC CTGGTTTGGT TATTTGCTTC CATTTTAATG 
CTTGGTGTTA CATTTATTAG TCAAGCTTTA ATAAACTTTG TTTGTAGTTT CGGCTTTCTT 
CCAGTCGTAA AGCTATTGTT AGGTGAAAGA AATAAGGTCT ATGTTGCATG GAAGGAACTC 
TCTGACATTA GCTACTCCTA TTTGAACATT TATTATGTCT GTGTCGGATC TGTTTGTTTA 
TCCAAGATTG CTAAGGATAT TCTTCATTTT ACAGAAGGCC AAAACACTCT CGATGAACAT 
GCCGTTGACG AAAACGAAGT AGAGGAAGTG GAACACGATA TACCAGAGAG AGATATCAAC 
AATGCCCCTG TTAATAATAT CAATAATGTG GAAGAGGGTC AAGGAATTTT CATGGCCATC 
TTTAACAGTA TATTTGATAG TATGTTGGTG AAGTATAACC TGATGGTGTT TATAGCTATC 
ATGATTGCGG TTATTAGAAC TATGGTTTCG TGGGTCGTCT TAACCGATGG TATACTAGCC 
TGTTACAATT ATCTCACCAT CAGAGTGTTT GGCAATTCTT CATACACTAT TGGTAATTCC 
AAATGGTTTA AATATGACGA GAGTTTGTTA TTCGTCGTTT GGATCATTTC GTCAATGGTC 
AATTTCGGAA CCGGATATAA GTCATTGAAA CTATTTTTCA GAAACAGAAA TACTAGTAAA 
TTGAACTTCT TGAAAACAAT GGCTTTGGAA CTGTTTAAAC AAGGTTTCCT GCACATGGTA 
ATTTACGTCT TGCCGATTAT TATTTTATCC TTAGTATTTT TACGTGATGT TTCTACAAAG 
CAGATTATCG ATATTAGCCA TGGATCCAGG AGTTTCACTT TATCGCTTAA CGAATCATTC 
CCAACATGGA CAAGGATGCA AGATATTTAT TTCGGACTAC TGATAGCATT AGAGTCATTT 
ACGTTCTTTT TCCAAGCAAC TGTGCTGTTC ATTCAATGGT TCAAAAGTAC TGTACAGAAT 
GTAAAAGACG AGGTTTACAC TAAGGGTAGA GCTTTAGAAA ATTTACCAGA TGAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SSM4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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