PRT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRT1
Entrez Gene ID	854543
Full Name	translation initiation factor eIF3 core subunit b
Synonyms	CDC63,DNA26
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183781.3	NP_015006.3	translation initiation factor eIF3 core subunit b

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EIF3B	NP_001095826.1
Mus musculus (house mouse)	Eif3b	NP_598677.1
Rattus norvegicus (Norway rat)	Eif3b	NP_001026810.1
Magnaporthe oryzae (rice blast fungus)	MGG_01978	XP_003708716.1
Oryza sativa (rice)	Os10g0569200	NP_001065446.1
Gallus gallus (chicken)	EIF3B	XP_003642190.2
Arabidopsis thaliana (thale cress)	EIF3B-2	NP_568477.1
Anopheles gambiae (African malaria mosquito)	EIF3B_ANOGA	XP_320387.3
Eremothecium gossypii	AGOS_ADR399C	NP_984494.1
Arabidopsis thaliana (thale cress)	TIF3B1	NP_568498.1
Pan troglodytes (chimpanzee)	EIF3B	XP_003318263.1
Kluyveromyces lactis	KLLA0A03531g	XP_451155.1
Neurospora crassa	NCU02208	XP_959527.1
Homo sapiens (human)	EIF3B	NP_001032360.1
	EIF3B	XP_001086876.1
Canis lupus familiaris (dog)	EIF3B	XP_536894.3
Danio rerio (zebrafish)	eif3ba	XP_001335480.2
Drosophila melanogaster (fruit fly)	eIF3-S9	NP_725691.1
Saccharomyces cerevisiae (baker's yeast)	PRT1	NP_015006.3
Schizosaccharomyces pombe (fission yeast)	SPAC25G10.08	NP_594528.1
Xenopus tropicalis (tropical clawed frog)	eif3b	NP_001016724.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that the eIF3b-eIF3j/eIF3a module binds near the mRNA entry channel and regulates the transition between scanning-conducive and initiation-competent conformations of the preinitiation complex.
Title: The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.

Yeast eIF3b exhibits RNA binding activity but huamn eIF3b doesn't.
Title: Crystal structure of the RNA recognition motif of yeast translation initiation factor eIF3b reveals differences to human eIF3b.

eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RNA recognition motif and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition
Title: The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection.

Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eIF3.
Title: Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast.

The following PRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03645	NM_001183781.3 Latest version!	Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit b (PRT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRT1 ( NM_001183781.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015006.3 Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit b (PRT1), partial mRNA.

Price & Availability↑

CloneID	OSi03645
Clone ID Related Accession (Same CDS sequence)	NM_001183781.3
Accession Version	NM_001183781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation initiation factor eIF3 core subunit b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183781.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAAATT TTCTTCCACG CACATTGAAA AATATTTACG AATTATATTT CAATAACATC 
TCAGTACATA GTATAGTATC TCGCAACACA CAGCTCAAAA GGTCAAAGAT AATTCAAATG 
ACTACCGAGA CTTTCGAAGA CATTAAGCTA GAGGACATCC CTGTTGACGA CATTGATTTT 
TCCGACCTGG AAGAACAATA CAAGGTCACT GAAGAATTCA ATTTCGATCA GTACATCGTC 
GTTAATGGTG CCCCAGTCAT CCCATCCGCC AAAGTTCCTG TTTTGAAAAA GGCTTTGACT 
TCTTTGTTTT CCAAAGCTGG TAAAGTTGTT AACATGGAAT TTCCAATTGA TGAAGCCACT 
GGTAAGACGA AAGGTTTTCT CTTCGTGGAA TGTGGCTCAA TGAACGATGC TAAAAAAATT 
ATCAAGAGTT TCCACGGTAA AAGACTGGAT TTAAAACATC GTTTGTTTCT TTATACTATG 
AAAGATGTTG AAAGATATAA TTCTGACGAC TTTGACACCG AATTCAGGGA GCCTGATATG 
CCTACATTCG TTCCATCTAG TTCCTTAAAA TCCTGGTTAA TGGACGACAA AGTGAGAGAT 
CAGTTTGTAT TACAAGATGA CGTGAAGACT AGTGTGTTCT GGAACTCTAT GTTCAATGAA 
GAAGATTCTT TGGTGGAATC TAGAGAGAAC TGGTCTACCA ATTATGTTAG ATTCTCCCCA 
AAGGGTACCT ATTTGTTTTC TTACCATCAA CAGGGTGTAA CTGCATGGGG TGGTCCAAAC 
TTCGATCGTT TGAGAAGATT CTATCATCCA GATGTAAGAA ACTCTTCCGT CTCTCCGAAT 
GAAAAGTACC TGGTCACTTT CTCCACGGAA CCAATCATTG TAGAAGAAGA TAACGAATTC 
TCTCCATTTA CCAAGAAAAA TGAGGGTCAT CAATTATGCA TCTGGGACAT TGCTTCTGGT 
CTGTTGATGG CAACTTTCCC AGTGATAAAG AGCCCATATC TGAAATGGCC TTTAGTTAGA 
TGGTCTTATA ATGATAAATA TTGTGCTCGT ATGGTTGGAG ACAGTTTAAT AGTTCACGAT 
GCTACTAAAA ACTTCATGCC ATTAGAAGCT AAGGCTTTGA AGCCTTCTGG TATTAGAGAT 
TTCTCATTTG CTCCAGAAGG TGTTAAATTA CAACCATTCA GAAACGGTGA CGAGCCTTCT 
GTTTTATTGG CTTATTGGAC TCCTGAGACT AATAATTCCG CTTGTACTGC TACCATTGCT 
GAAGTTCCTC GCGGTAGAGT GCTAAAAACC GTTAACTTAG TTCAAGTCTC CAACGTAACT 
CTGCACTGGC AAAACCAAGC TGAGTTCCTG TGCTTCAATG TCGAACGTCA CACAAAGTCT 
GGTAAGACTC AATTCAGTAA TCTACAAATT TGTAGGTTGA CTGAAAGAGA TATACCAGTT 
GAAAAGGTTG AATTGAAAGA TAGTGTCTTT GAATTCGGTT GGGAACCCCA CGGAAATAGA 
TTTGTTACAA TTTCTGTTCA TGAAGTAGCT GATATGAATT ATGCTATCCC AGCAAATACA 
ATCAGATTTT ACGCTCCAGA AACTAAAGAA AAGACTGACG TAATTAAAAG ATGGAGCTTG 
GTCAAAGAAA TTCCAAAAAC TTTTGCCAAC ACTGTTTCTT GGTCTCCAGC GGGTAGATTT 
GTGGTTGTAG GTGCTTTAGT GGGTCCAAAC ATGCGTAGAT CAGACCTACA ATTTTACGAT 
ATGGACTATC CAGGTGAAAA GAATATTAAT GACAATAATG ATGTTTCAGC TTCTTTGAAA 
GATGTTGCTC ATCCTACCTA CTCTGCGGCT ACTAATATCA CTTGGGATCC ATCTGGTAGA 
TACGTTACCG CATGGTCAAG TTCTTTGAAA CACAAGGTGG AGCATGGTTA CAAAATTTTC 
AATATTGCTG GTAACTTAGT TAAAGAAGAC ATCATTGCTG GCTTCAAGAA CTTTGCCTGG 
AGACCAAGGC CAGCTTCCAT TTTGTCTAAC GCCGAAAGGA AAAAGGTTAG GAAAAACTTG 
AGGGAATGGT CTGCCCAATT CGAAGAGCAA GATGCAATGG AAGCTGACAC CGCCATGAGA 
GATTTGATTC TACACCAACG TGAATTATTG AAGCAATGGA CCGAATATAG AGAAAAAATT 
GGCCAAGAAA TGGAAAAATC TATGAATTTC AAAATCTTTG ACGTTCAACC AGAAGATGCC 
AGCGATGACT TTACCACCAT CGAAGAGATA GTTGAAGAAG TTTTGGAAGA AACAAAGGAA 
AAGGTCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015006.3
CDS	1..2292
Translation	MKNFLPRTLKNIYELYFNNISVHSIVSRNTQLKRSKIIQMTTETFEDIKLEDIPVDDIDFSDLEEQYKVTEEFNFDQYIVVNGAPVIPSAKVPVLKKALTSLFSKAGKVVNMEFPIDEATGKTKGFLFVECGSMNDAKKIIKSFHGKRLDLKHRLFLYTMKDVERYNSDDFDTEFREPDMPTFVPSSSLKSWLMDDKVRDQFVLQDDVKTSVFWNSMFNEEDSLVESRENWSTNYVRFSPKGTYLFSYHQQGVTAWGGPNFDRLRRFYHPDVRNSSVSPNEKYLVTFSTEPIIVEEDNEFSPFTKKNEGHQLCIWDIASGLLMATFPVIKSPYLKWPLVRWSYNDKYCARMVGDSLIVHDATKNFMPLEAKALKPSGIRDFSFAPEGVKLQPFRNGDEPSVLLAYWTPETNNSACTATIAEVPRGRVLKTVNLVQVSNVTLHWQNQAEFLCFNVERHTKSGKTQFSNLQICRLTERDIPVEKVELKDSVFEFGWEPHGNRFVTISVHEVADMNYAIPANTIRFYAPETKEKTDVIKRWSLVKEIPKTFANTVSWSPAGRFVVVGALVGPNMRRSDLQFYDMDYPGEKNINDNNDVSASLKDVAHPTYSAATNITWDPSGRYVTAWSSSLKHKVEHGYKIFNIAGNLVKEDIIAGFKNFAWRPRPASILSNAERKKVRKNLREWSAQFEEQDAMEADTAMRDLILHQRELLKQWTEYREKIGQEMEKSMNFKIFDVQPEDASDDFTTIEEIVEEVLEETKEKVE

Target ORF information:

RefSeq Version	NM_001183781.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit b (PRT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183781.3

ORF Insert Sequence:

ATGAAAAATT TTCTTCCACG CACATTGAAA AATATTTACG AATTATATTT CAATAACATC 
TCAGTACATA GTATAGTATC TCGCAACACA CAGCTCAAAA GGTCAAAGAT AATTCAAATG 
ACTACCGAGA CTTTCGAAGA CATTAAGCTA GAGGACATCC CTGTTGACGA CATTGATTTT 
TCCGACCTGG AAGAACAATA CAAGGTCACT GAAGAATTCA ATTTCGATCA GTACATCGTC 
GTTAATGGTG CCCCAGTCAT CCCATCCGCC AAAGTTCCTG TTTTGAAAAA GGCTTTGACT 
TCTTTGTTTT CCAAAGCTGG TAAAGTTGTT AACATGGAAT TTCCAATTGA TGAAGCCACT 
GGTAAGACGA AAGGTTTTCT CTTCGTGGAA TGTGGCTCAA TGAACGATGC TAAAAAAATT 
ATCAAGAGTT TCCACGGTAA AAGACTGGAT TTAAAACATC GTTTGTTTCT TTATACTATG 
AAAGATGTTG AAAGATATAA TTCTGACGAC TTTGACACCG AATTCAGGGA GCCTGATATG 
CCTACATTCG TTCCATCTAG TTCCTTAAAA TCCTGGTTAA TGGACGACAA AGTGAGAGAT 
CAGTTTGTAT TACAAGATGA CGTGAAGACT AGTGTGTTCT GGAACTCTAT GTTCAATGAA 
GAAGATTCTT TGGTGGAATC TAGAGAGAAC TGGTCTACCA ATTATGTTAG ATTCTCCCCA 
AAGGGTACCT ATTTGTTTTC TTACCATCAA CAGGGTGTAA CTGCATGGGG TGGTCCAAAC 
TTCGATCGTT TGAGAAGATT CTATCATCCA GATGTAAGAA ACTCTTCCGT CTCTCCGAAT 
GAAAAGTACC TGGTCACTTT CTCCACGGAA CCAATCATTG TAGAAGAAGA TAACGAATTC 
TCTCCATTTA CCAAGAAAAA TGAGGGTCAT CAATTATGCA TCTGGGACAT TGCTTCTGGT 
CTGTTGATGG CAACTTTCCC AGTGATAAAG AGCCCATATC TGAAATGGCC TTTAGTTAGA 
TGGTCTTATA ATGATAAATA TTGTGCTCGT ATGGTTGGAG ACAGTTTAAT AGTTCACGAT 
GCTACTAAAA ACTTCATGCC ATTAGAAGCT AAGGCTTTGA AGCCTTCTGG TATTAGAGAT 
TTCTCATTTG CTCCAGAAGG TGTTAAATTA CAACCATTCA GAAACGGTGA CGAGCCTTCT 
GTTTTATTGG CTTATTGGAC TCCTGAGACT AATAATTCCG CTTGTACTGC TACCATTGCT 
GAAGTTCCTC GCGGTAGAGT GCTAAAAACC GTTAACTTAG TTCAAGTCTC CAACGTAACT 
CTGCACTGGC AAAACCAAGC TGAGTTCCTG TGCTTCAATG TCGAACGTCA CACAAAGTCT 
GGTAAGACTC AATTCAGTAA TCTACAAATT TGTAGGTTGA CTGAAAGAGA TATACCAGTT 
GAAAAGGTTG AATTGAAAGA TAGTGTCTTT GAATTCGGTT GGGAACCCCA CGGAAATAGA 
TTTGTTACAA TTTCTGTTCA TGAAGTAGCT GATATGAATT ATGCTATCCC AGCAAATACA 
ATCAGATTTT ACGCTCCAGA AACTAAAGAA AAGACTGACG TAATTAAAAG ATGGAGCTTG 
GTCAAAGAAA TTCCAAAAAC TTTTGCCAAC ACTGTTTCTT GGTCTCCAGC GGGTAGATTT 
GTGGTTGTAG GTGCTTTAGT GGGTCCAAAC ATGCGTAGAT CAGACCTACA ATTTTACGAT 
ATGGACTATC CAGGTGAAAA GAATATTAAT GACAATAATG ATGTTTCAGC TTCTTTGAAA 
GATGTTGCTC ATCCTACCTA CTCTGCGGCT ACTAATATCA CTTGGGATCC ATCTGGTAGA 
TACGTTACCG CATGGTCAAG TTCTTTGAAA CACAAGGTGG AGCATGGTTA CAAAATTTTC 
AATATTGCTG GTAACTTAGT TAAAGAAGAC ATCATTGCTG GCTTCAAGAA CTTTGCCTGG 
AGACCAAGGC CAGCTTCCAT TTTGTCTAAC GCCGAAAGGA AAAAGGTTAG GAAAAACTTG 
AGGGAATGGT CTGCCCAATT CGAAGAGCAA GATGCAATGG AAGCTGACAC CGCCATGAGA 
GATTTGATTC TACACCAACG TGAATTATTG AAGCAATGGA CCGAATATAG AGAAAAAATT 
GGCCAAGAAA TGGAAAAATC TATGAATTTC AAAATCTTTG ACGTTCAACC AGAAGATGCC 
AGCGATGACT TTACCACCAT CGAAGAGATA GTTGAAGAAG TTTTGGAAGA AACAAAGGAA 
AAGGTCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PRT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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