KIN4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	KIN4
Entrez Gene ID	854408
Full Name	putative serine/threonine protein kinase KIN4
Synonyms	KIN3,KIN31
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183652.1	NP_014876.1	putative serine/threonine protein kinase KIN4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that bud-localized MEN regulator Lte1 prevents Kin4 that escapes into the bud from associating with SPBs in the daughter cell.
Title: Lte1 promotes mitotic exit by controlling the localization of the spindle position checkpoint kinase Kin4.

Data suggest that Lte1 promotes mitotic exit by inhibiting Kin4 activity at the daughter spindle pole body.
Title: The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4.

The bud neck-associated kinase Elm1 participates in Kin4 activation and SPOC signaling by phosphorylating a conserved residue within the activation loop of Kin4.
Title: Elm1 kinase activates the spindle position checkpoint kinase Kin4.

show that the protein phosphatase functions upstream of Kin4, regulating the kinase's phosphorylation and localization during an unperturbed cell cycle and during SPOC activation, thus defining the phosphatase as a key regulator of SPOC function
Title: The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4.

The following KIN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03529	NM_001183652.1 Latest version!	Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KIN4 (KIN4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIN4 ( NM_001183652.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014876.1 Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KIN4 (KIN4), partial mRNA.

Price & Availability↑

CloneID	OSi03529
Clone ID Related Accession (Same CDS sequence)	NM_001183652.1
Accession Version	NM_001183652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative serine/threonine protein kinase KIN4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTTCTG TACCTAAACG CCATACATAC GGTGGCAACG TTGTCACTGA TAGGGACCGT 
CATTCTCTTC AAAGAAACAA TGAGATTTTG CATCCTATAC ATAAAAATCA GCGAAAACAT 
GCTACCTTCG GACCATACAT AATCGGTTCC ACTTTGGGTG AGGGAGAATT TGGTAAAGTT 
AAGCTGGGTT GGACCAAGGC TTCCTCCTCG AACGAGGTTC CTAAGCAAGT GGCAATCAAA 
TTAATTAGAA GAGATACAAT CAAGAAAGAT GCCGATAAAG AAATTAAAAT ATACCGCGAA 
ATTAACGCAC TGAAGCATTT AACTCATCCT AACATTATCT ATTTAGAAGA GGTCCTGCAG 
AATTCGAAAT ACATTGGAAT AGTGCTAGAG TTTGTATCTG GGGGAGAATT CTATAAGTAT 
ATTCAACGTA AGAGGAGATT AAAGGAATCT TCTGCATGCA GATTGTTTGC CCAATTAATT 
AGTGGGGTCA ATTATATGCA CTACAAAGGA CTTGTTCATA GGGACTTAAA ACTGGAAAAT 
TTATTATTAG ATAAGCACGA AAATTTAGTC ATCACGGATT TTGGTTTTGT GAATGAATTT 
TTTGAAGATA ACGAATTAAT GAAAACTTCT TGTGGTTCGC CCTGTTATGC AGCACCAGAA 
CTAGTTGTTA GTACCAAGGC ATATGAAGCA AGAAAGGCAG ATGTTTGGTC GTGTGGTGTT 
ATTCTTTATG CAATGCTCGC TGGATATTTG CCGTGGGATG ACGATCATGA AAATCCAACG 
GGTGACGATA TTGCTAGACT GTACAAATAC ATCACGCAAA CACCTCTTAA ATTTCCCGAA 
TATATAACCC CTATTCCAAG AGATTTGCTG AGGCGAATAC TGGTACCCAA TCCAAGAAGA 
AGAATAAACT TACAAACAAT AAAAAGGCAT GTGTGGTTGA AGCCTCATGA AGCGTTTTTG 
AGCATTCAGC CGAACTATTG GGATGAACAC TTACAGAAGG AACGTCCGAA ACCGCCAAAC 
AAAGGTGACG TTGGTCGCCA CTCAACTTAT TCTTCATCAG CATCTTCGTA CTCAAAAAGT 
AGGGATAGAA ACTCTTTAAT TATAGAGTCG ACTCTAGAGC AACATCGAAT GTCTCCACAG 
CTTGCGACCA GCAGACCAGC ATCACCAACA TTCTCTACTG GTTCCAAAGT GGTGTTAAAC 
GATACGAAAA ATGACATGAA AGAATCTAAT ATAAATGGCG AGCGTACTAG TGCATCATGC 
AGGTACACAC GTGATTCGAA GGGCAACGGC CAGACCCAAA TAGAACAAGT ATCAGCTAGA 
CACTCATCCA GAGGCAATAA GCACACATCG GTCGCGGGAC TCGTAACAAT CCCTGGCTCC 
CCCACAACTG CAAGAACAAG GAATGCACCA TCATCAAAGC TTACTGAGCA TGTAAAAGAT 
TCTAGTCAGA CAAGCTTTAC TCAGGAGGAA TTCCACCGTA TTGGTAATTA TCACGTGCCT 
CGCAGTAGAC CTAGGCCAAC TTCATATTAT CCTGGACTCA GTCGTAACAC CGCGGATAAT 
AGTTTAGCGG ATATTCCAGT AAATAAGTTG GGTTCGAATG GTAGGTTGAC AGATGCCAAA 
GACCCGGTAC CGTTGAACGC AATACACGAT ACCAACAAAG CTACAATCTC CAACAATTCC 
ATTATGCTAT TATCAGAAGG TCCGGCTGCA AAGACTTCCC CAGTAGATTA CCATTATGCT 
ATTGGCGACT TAAACCATGG TGACAAACCC ATAACTGAGG TTATCGACAA AATTAATAAG 
GACCTTACTC ATAAAGCAGC TGAAAATGGT TTTCCTCGAG AGAGCATCGA CCCAGAGAGT 
ACCTCAACGA TATTAGTTAC GAAAGAACCC ACTAACTCCA CCGATGAAGA TCATGTAGAA 
TCTCAGCTGG AGAATGTTGG CCACTCTTCT AATAAATCAG ACGCTTCTTC TGATAAAGAT 
AGTAAAAAGA TCTACGAAAA AAAAAGGTTC AGCTTCATGT CGTTGTACTC ATCCTTAAAT 
GGTTCAAGAT CCACTGTTGA ATCTCGTACC TCAAAAGGGA ATGCACCTCC TGTTTCATCT 
AGAAATCCAT CAGGACAATC GAATAGAAGT AACATTAAAA TAACTCAGCA GCAACCACGT 
AACCTTTCCG ACAGAGTTCC AAATCCCGAT AAGAAAATTA ATGATAATAG AATAAGAGAC 
AACGCACCTA GCTATGCTGA AAGCGAGAAT CCGGGAAGGT CTGTACGGGC TTCTGTTATG 
GTGTCAACAC TACGAGAAGA AAATCGATCG GAATTGTCGA ATGAAGGGAA TAATGTCGAG 
GCACAGACTT CAACAGCAAG AAAGGTTCTG AATTTTTTCA AAAGAAGGAG CATGAGGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014876.1
CDS	1..2403
Translation	MASVPKRHTYGGNVVTDRDRHSLQRNNEILHPIHKNQRKHATFGPYIIGSTLGEGEFGKVKLGWTKASSSNEVPKQVAIKLIRRDTIKKDADKEIKIYREINALKHLTHPNIIYLEEVLQNSKYIGIVLEFVSGGEFYKYIQRKRRLKESSACRLFAQLISGVNYMHYKGLVHRDLKLENLLLDKHENLVITDFGFVNEFFEDNELMKTSCGSPCYAAPELVVSTKAYEARKADVWSCGVILYAMLAGYLPWDDDHENPTGDDIARLYKYITQTPLKFPEYITPIPRDLLRRILVPNPRRRINLQTIKRHVWLKPHEAFLSIQPNYWDEHLQKERPKPPNKGDVGRHSTYSSSASSYSKSRDRNSLIIESTLEQHRMSPQLATSRPASPTFSTGSKVVLNDTKNDMKESNINGERTSASCRYTRDSKGNGQTQIEQVSARHSSRGNKHTSVAGLVTIPGSPTTARTRNAPSSKLTEHVKDSSQTSFTQEEFHRIGNYHVPRSRPRPTSYYPGLSRNTADNSLADIPVNKLGSNGRLTDAKDPVPLNAIHDTNKATISNNSIMLLSEGPAAKTSPVDYHYAIGDLNHGDKPITEVIDKINKDLTHKAAENGFPRESIDPESTSTILVTKEPTNSTDEDHVESQLENVGHSSNKSDASSDKDSKKIYEKKRFSFMSLYSSLNGSRSTVESRTSKGNAPPVSSRNPSGQSNRSNIKITQQQPRNLSDRVPNPDKKINDNRIRDNAPSYAESENPGRSVRASVMVSTLREENRSELSNEGNNVEAQTSTARKVLNFFKRRSMRV

Target ORF information:

RefSeq Version	NM_001183652.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KIN4 (KIN4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183652.1

ORF Insert Sequence:

ATGGCTTCTG TACCTAAACG CCATACATAC GGTGGCAACG TTGTCACTGA TAGGGACCGT 
CATTCTCTTC AAAGAAACAA TGAGATTTTG CATCCTATAC ATAAAAATCA GCGAAAACAT 
GCTACCTTCG GACCATACAT AATCGGTTCC ACTTTGGGTG AGGGAGAATT TGGTAAAGTT 
AAGCTGGGTT GGACCAAGGC TTCCTCCTCG AACGAGGTTC CTAAGCAAGT GGCAATCAAA 
TTAATTAGAA GAGATACAAT CAAGAAAGAT GCCGATAAAG AAATTAAAAT ATACCGCGAA 
ATTAACGCAC TGAAGCATTT AACTCATCCT AACATTATCT ATTTAGAAGA GGTCCTGCAG 
AATTCGAAAT ACATTGGAAT AGTGCTAGAG TTTGTATCTG GGGGAGAATT CTATAAGTAT 
ATTCAACGTA AGAGGAGATT AAAGGAATCT TCTGCATGCA GATTGTTTGC CCAATTAATT 
AGTGGGGTCA ATTATATGCA CTACAAAGGA CTTGTTCATA GGGACTTAAA ACTGGAAAAT 
TTATTATTAG ATAAGCACGA AAATTTAGTC ATCACGGATT TTGGTTTTGT GAATGAATTT 
TTTGAAGATA ACGAATTAAT GAAAACTTCT TGTGGTTCGC CCTGTTATGC AGCACCAGAA 
CTAGTTGTTA GTACCAAGGC ATATGAAGCA AGAAAGGCAG ATGTTTGGTC GTGTGGTGTT 
ATTCTTTATG CAATGCTCGC TGGATATTTG CCGTGGGATG ACGATCATGA AAATCCAACG 
GGTGACGATA TTGCTAGACT GTACAAATAC ATCACGCAAA CACCTCTTAA ATTTCCCGAA 
TATATAACCC CTATTCCAAG AGATTTGCTG AGGCGAATAC TGGTACCCAA TCCAAGAAGA 
AGAATAAACT TACAAACAAT AAAAAGGCAT GTGTGGTTGA AGCCTCATGA AGCGTTTTTG 
AGCATTCAGC CGAACTATTG GGATGAACAC TTACAGAAGG AACGTCCGAA ACCGCCAAAC 
AAAGGTGACG TTGGTCGCCA CTCAACTTAT TCTTCATCAG CATCTTCGTA CTCAAAAAGT 
AGGGATAGAA ACTCTTTAAT TATAGAGTCG ACTCTAGAGC AACATCGAAT GTCTCCACAG 
CTTGCGACCA GCAGACCAGC ATCACCAACA TTCTCTACTG GTTCCAAAGT GGTGTTAAAC 
GATACGAAAA ATGACATGAA AGAATCTAAT ATAAATGGCG AGCGTACTAG TGCATCATGC 
AGGTACACAC GTGATTCGAA GGGCAACGGC CAGACCCAAA TAGAACAAGT ATCAGCTAGA 
CACTCATCCA GAGGCAATAA GCACACATCG GTCGCGGGAC TCGTAACAAT CCCTGGCTCC 
CCCACAACTG CAAGAACAAG GAATGCACCA TCATCAAAGC TTACTGAGCA TGTAAAAGAT 
TCTAGTCAGA CAAGCTTTAC TCAGGAGGAA TTCCACCGTA TTGGTAATTA TCACGTGCCT 
CGCAGTAGAC CTAGGCCAAC TTCATATTAT CCTGGACTCA GTCGTAACAC CGCGGATAAT 
AGTTTAGCGG ATATTCCAGT AAATAAGTTG GGTTCGAATG GTAGGTTGAC AGATGCCAAA 
GACCCGGTAC CGTTGAACGC AATACACGAT ACCAACAAAG CTACAATCTC CAACAATTCC 
ATTATGCTAT TATCAGAAGG TCCGGCTGCA AAGACTTCCC CAGTAGATTA CCATTATGCT 
ATTGGCGACT TAAACCATGG TGACAAACCC ATAACTGAGG TTATCGACAA AATTAATAAG 
GACCTTACTC ATAAAGCAGC TGAAAATGGT TTTCCTCGAG AGAGCATCGA CCCAGAGAGT 
ACCTCAACGA TATTAGTTAC GAAAGAACCC ACTAACTCCA CCGATGAAGA TCATGTAGAA 
TCTCAGCTGG AGAATGTTGG CCACTCTTCT AATAAATCAG ACGCTTCTTC TGATAAAGAT 
AGTAAAAAGA TCTACGAAAA AAAAAGGTTC AGCTTCATGT CGTTGTACTC ATCCTTAAAT 
GGTTCAAGAT CCACTGTTGA ATCTCGTACC TCAAAAGGGA ATGCACCTCC TGTTTCATCT 
AGAAATCCAT CAGGACAATC GAATAGAAGT AACATTAAAA TAACTCAGCA GCAACCACGT 
AACCTTTCCG ACAGAGTTCC AAATCCCGAT AAGAAAATTA ATGATAATAG AATAAGAGAC 
AACGCACCTA GCTATGCTGA AAGCGAGAAT CCGGGAAGGT CTGTACGGGC TTCTGTTATG 
GTGTCAACAC TACGAGAAGA AAATCGATCG GAATTGTCGA ATGAAGGGAA TAATGTCGAG 
GCACAGACTT CAACAGCAAG AAAGGTTCTG AATTTTTTCA AAAGAAGGAG CATGAGGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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KIN4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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