PAM17 cDNA ORF clone, Saccharomyces cerevisiae S288C

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
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Nature369(6479)371-8(1994 Jun)

The presequence translocase constituent Pam17 is specifically recruited by the receptor Tim50 to promote the transport of hypersensitive precursors into the mitochondrial matrix.
Title: Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation.

Tim23 mitochondrial motor components are tethered to translocon via the scaffold Tim44, with regulatory protein Pam17 having independent interaction site.
Title: Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor.

The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.
Title: Signal recognition initiates reorganization of the presequence translocase during protein import.

Genetic and biochemical analyses reveal synthetic interactions between PAM17 and TIM44.
Title: Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix.

Tim44 is not simply a scaffold for binding of motor subunits but plays a differential role in the recruitment of PAM modules to the inner membrane translocase.
Title: Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase.