TRM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TRM2
Entrez Gene ID	853930
Full Name	Trm2p
Synonyms	NUC2,NUD1,RNC1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179846.1	NP_012982.2	tRNA (uracil(54)-C(5))-methyltransferase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A05665g	XP_451251.1
Oryza sativa (rice)	Os01g0193600	NP_001042282.1
Eremothecium gossypii	AGOS_ACR256C	NP_983658.1
Schizosaccharomyces pombe (fission yeast)	SPAC4G8.07c	NP_593067.1
Neurospora crassa	NCU06780	XP_963621.1
Magnaporthe oryzae (rice blast fungus)	MGG_03881	XP_003719998.1
Arabidopsis thaliana (thale cress)	AT3G21300	NP_188767.2
Saccharomyces cerevisiae (baker's yeast)	TRM2	NP_012982.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A defective processing in absence of TRM2, with a synergistic effect of TRM2, and EXO1 in such DNA repair processing.
Title: Synergistic effect of TRM2/RNC1 and EXO1 in DNA double-strand break repair in Saccharomyces cerevisiae.

These results propose an important role for TRM2 in DNA repair with a potential involvement of its nuclease function in homologous recombination based repair of DNA double strand break.
Title: Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair.

The following TRM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03109	NM_001179846.1 Latest version!	Saccharomyces cerevisiae S288C tRNA (uracil(54)-C(5))-methyltransferase (TRM2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRM2 ( NM_001179846.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012982.2 Saccharomyces cerevisiae S288C tRNA (uracil(54)-C(5))-methyltransferase (TRM2), partial mRNA.

Price & Availability↑

CloneID	OSi03109
Clone ID Related Accession (Same CDS sequence)	NM_001179846.1
Accession Version	NM_001179846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tRNA (uracil(54)-C(5))-methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTACGAAC AGTTTGAATT TTCTTTTTTT TTCTTCGAAA ATTCAGACAA TAAGGTTAAA 
TATAAAGCTC ATCTCATCTC ATCGATAAAA CGCTGGAGTA TTATCACATG CATGCGTTGC 
TTTTGGACCG TACAGAAGTC TATATTTAAA GCTAGGTTTT TCGCTTGCAG AAACTTTGTC 
AAGAAGCATA ATTATAAACT AATCAGCACC ATGACTGGAA GTACTGAAAT GGTACCACCA 
ACAATGAAAC ATACCGTTGA CAACAAAAGG CTTTCGTCTC CTTTGACAGA TTCTGGTAAC 
CGCCGGACTA AGAAGCCAAA GTTGAGAAAG TACAAGGCCA AAAAGGTTGA AACAACTTCT 
CCGATGGGTG TCCTAGAATT TGAAGTGAAC GATTTGTTAA AATCTCAAAA TTTGTCCAGG 
GAGCAGGTTC TGAACGATGT TACTTCAATT CTAAATGATA AGTCCTCAAC AGATGGACCT 
ATCGTCTTAC AATATCACCG AGAGGTAAAA AATGTCAAGG TCTTAGAAAT TACTTCCAAT 
GGCAACGGGT TGGCTTTGAT CGATAATCCT GTTGAAACAG AAAAGAAGCA AGTTGTTATC 
ATACCGTTTG GCCTGCCCGG TGATGTAGTT AATATCAAAG TCTTTAAGAC CCACCCTTAC 
TATGTCGAGA GTGATTTATT AGACGTAGTG GAAAAATCTC CAATGAGAAG AGATGATTTA 
ATTAGGGATA AATATTTCGG GAAATCTTCA GGAAGTCAAT TAGAGTTCTT AACTTACGAT 
GACCAACTAG AATTGAAAAG AAAAACAATT ATGAATGCCT ACAAGTTCTT CGCACCAAGG 
TTAGTTGCTG AAAAACTTTT ACCCCCATTT GACACCACCG TAGCTTCCCC TTTACAATTT 
GGCTACAGGA CCAAAATTAC GCCTCATTTC GATATGCCAA AAAGAAAACA AAAGGAACTA 
TCAGTAAGGC CTCCTTTAGG ATTTGGTCAA AAGGGTAGAC CTCAATGGAG AAAAGATACT 
TTGGATATCG GCGGACATGG TTCGATATTA GATATCGATG AATGTGTGCT TGCAACTGAA 
GTTCTCAACA AAGGATTGAC TAATGAAAGA AGAAAGTTTG AGCAAGAGTT TAAAAACTAC 
AAAAAAGGCG CGACTATTTT ACTGAGAGAG AATACCACTA TTTTAGACCC TTCCAAACCA 
ACTTTAGAAC AGTTAACCGA AGAAGCCTCT AGGGATGAAA ATGGTGATAT AAGTTATGTC 
GAAGTCGAAG ACAAAAAGAA CAATGTCAGG CTGGCTAAAA CTTGCGTTAC CAATCCTAGA 
CAAATTGTCA CTGAATATGT TGATGGATAT ACTTTTAATT TTAGTGCGGG TGAGTTTTTT 
CAAAATAATA ACTCTATCCT GCCAATAGTG ACCAAGTATG TCCGTGATAA CTTGCAAGCT 
CCCGCTAAAG GTGATGATAA TAAAACAAAG TTTCTAGTAG ATGCTTATTG TGGATCAGGT 
CTTTTCAGTA TATGCAGCTC CAAGGGCGTA GATAAAGTGA TTGGTGTAGA AATTTCCGCT 
GACAGTGTCT CTTTTGCAGA AAAAAATGCA AAGGCAAATG GTGTTGAAAA CTGTAGATTC 
ATCGTTGGAA AGGCTGAGAA ACTCTTTGAG TCTATTGATA CTCCAAGTGA AAACACTTCC 
GTTATCTTGG ATCCACCACG CAAGGGCTGT GACGAATTAT TCCTAAAGCA ATTAGCCGCA 
TATAATCCAG CCAAGATTAT TTACATATCG TGTAATGTCC ATTCCCAGGC ACGTGATGTC 
GAGTACTTCC TCAAAGAAAC AGAAAACGGT TCCGCCCACC AGATTGAAAG CATAAGAGGA 
TTTGATTTCT TTCCACAAAC GCACCACGTT GAGAGTGTGT GTATAATGAA GAGAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012982.2
CDS	1..1920
Translation	MYEQFEFSFFFFENSDNKVKYKAHLISSIKRWSIITCMRCFWTVQKSIFKARFFACRNFVKKHNYKLISTMTGSTEMVPPTMKHTVDNKRLSSPLTDSGNRRTKKPKLRKYKAKKVETTSPMGVLEFEVNDLLKSQNLSREQVLNDVTSILNDKSSTDGPIVLQYHREVKNVKVLEITSNGNGLALIDNPVETEKKQVVIIPFGLPGDVVNIKVFKTHPYYVESDLLDVVEKSPMRRDDLIRDKYFGKSSGSQLEFLTYDDQLELKRKTIMNAYKFFAPRLVAEKLLPPFDTTVASPLQFGYRTKITPHFDMPKRKQKELSVRPPLGFGQKGRPQWRKDTLDIGGHGSILDIDECVLATEVLNKGLTNERRKFEQEFKNYKKGATILLRENTTILDPSKPTLEQLTEEASRDENGDISYVEVEDKKNNVRLAKTCVTNPRQIVTEYVDGYTFNFSAGEFFQNNNSILPIVTKYVRDNLQAPAKGDDNKTKFLVDAYCGSGLFSICSSKGVDKVIGVEISADSVSFAEKNAKANGVENCRFIVGKAEKLFESIDTPSENTSVILDPPRKGCDELFLKQLAAYNPAKIIYISCNVHSQARDVEYFLKETENGSAHQIESIRGFDFFPQTHHVESVCIMKRI

Target ORF information:

RefSeq Version	NM_001179846.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tRNA (uracil(54)-C(5))-methyltransferase (TRM2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179846.1

ORF Insert Sequence:

ATGTACGAAC AGTTTGAATT TTCTTTTTTT TTCTTCGAAA ATTCAGACAA TAAGGTTAAA 
TATAAAGCTC ATCTCATCTC ATCGATAAAA CGCTGGAGTA TTATCACATG CATGCGTTGC 
TTTTGGACCG TACAGAAGTC TATATTTAAA GCTAGGTTTT TCGCTTGCAG AAACTTTGTC 
AAGAAGCATA ATTATAAACT AATCAGCACC ATGACTGGAA GTACTGAAAT GGTACCACCA 
ACAATGAAAC ATACCGTTGA CAACAAAAGG CTTTCGTCTC CTTTGACAGA TTCTGGTAAC 
CGCCGGACTA AGAAGCCAAA GTTGAGAAAG TACAAGGCCA AAAAGGTTGA AACAACTTCT 
CCGATGGGTG TCCTAGAATT TGAAGTGAAC GATTTGTTAA AATCTCAAAA TTTGTCCAGG 
GAGCAGGTTC TGAACGATGT TACTTCAATT CTAAATGATA AGTCCTCAAC AGATGGACCT 
ATCGTCTTAC AATATCACCG AGAGGTAAAA AATGTCAAGG TCTTAGAAAT TACTTCCAAT 
GGCAACGGGT TGGCTTTGAT CGATAATCCT GTTGAAACAG AAAAGAAGCA AGTTGTTATC 
ATACCGTTTG GCCTGCCCGG TGATGTAGTT AATATCAAAG TCTTTAAGAC CCACCCTTAC 
TATGTCGAGA GTGATTTATT AGACGTAGTG GAAAAATCTC CAATGAGAAG AGATGATTTA 
ATTAGGGATA AATATTTCGG GAAATCTTCA GGAAGTCAAT TAGAGTTCTT AACTTACGAT 
GACCAACTAG AATTGAAAAG AAAAACAATT ATGAATGCCT ACAAGTTCTT CGCACCAAGG 
TTAGTTGCTG AAAAACTTTT ACCCCCATTT GACACCACCG TAGCTTCCCC TTTACAATTT 
GGCTACAGGA CCAAAATTAC GCCTCATTTC GATATGCCAA AAAGAAAACA AAAGGAACTA 
TCAGTAAGGC CTCCTTTAGG ATTTGGTCAA AAGGGTAGAC CTCAATGGAG AAAAGATACT 
TTGGATATCG GCGGACATGG TTCGATATTA GATATCGATG AATGTGTGCT TGCAACTGAA 
GTTCTCAACA AAGGATTGAC TAATGAAAGA AGAAAGTTTG AGCAAGAGTT TAAAAACTAC 
AAAAAAGGCG CGACTATTTT ACTGAGAGAG AATACCACTA TTTTAGACCC TTCCAAACCA 
ACTTTAGAAC AGTTAACCGA AGAAGCCTCT AGGGATGAAA ATGGTGATAT AAGTTATGTC 
GAAGTCGAAG ACAAAAAGAA CAATGTCAGG CTGGCTAAAA CTTGCGTTAC CAATCCTAGA 
CAAATTGTCA CTGAATATGT TGATGGATAT ACTTTTAATT TTAGTGCGGG TGAGTTTTTT 
CAAAATAATA ACTCTATCCT GCCAATAGTG ACCAAGTATG TCCGTGATAA CTTGCAAGCT 
CCCGCTAAAG GTGATGATAA TAAAACAAAG TTTCTAGTAG ATGCTTATTG TGGATCAGGT 
CTTTTCAGTA TATGCAGCTC CAAGGGCGTA GATAAAGTGA TTGGTGTAGA AATTTCCGCT 
GACAGTGTCT CTTTTGCAGA AAAAAATGCA AAGGCAAATG GTGTTGAAAA CTGTAGATTC 
ATCGTTGGAA AGGCTGAGAA ACTCTTTGAG TCTATTGATA CTCCAAGTGA AAACACTTCC 
GTTATCTTGG ATCCACCACG CAAGGGCTGT GACGAATTAT TCCTAAAGCA ATTAGCCGCA 
TATAATCCAG CCAAGATTAT TTACATATCG TGTAATGTCC ATTCCCAGGC ACGTGATGTC 
GAGTACTTCC TCAAAGAAAC AGAAAACGGT TCCGCCCACC AGATTGAAAG CATAAGAGGA 
TTTGATTTCT TTCCACAAAC GCACCACGTT GAGAGTGTGT GTATAATGAA GAGAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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TRM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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