DEF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
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Nature369(6479)371-8(1994 Jun)

Def1, known to promote ubiquitination and degradation of stalled RNA polymerase complex, also directs NER to AP lesions on the transcribed strand of an actively transcribed gene but that its function is dependent on metabolic state of the yeast cells.
Title: Def1 and Dst1 play distinct roles in repair of AP lesions in highly transcribed genomic regions.

TLS polymerase REV1 carries out DNA lesion bypass only after the Def1-assisted removal of Pol3 from the stalled replication fork.
Title: Def1 promotes the degradation of Pol3 for polymerase exchange to occur during DNA-damage--induced mutagenesis in Saccharomyces cerevisiae.

These results indicate that Def1 bridges the interaction between RNAPII and the Elongin-Cullin ubiquitin ligase complex via an interaction between its CUE domain and a UbH in the Ela1 subunit of the Ela1-Elc1 adaptor complex.
Title: Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress.

Def1p plays multiple roles in yeast, including telomere maintenance
Title: Def1p is involved in telomere maintenance in budding yeast.