EBP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	EBP2
Entrez Gene ID	853682
Full Name	Ebp2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179738.1	NP_012749.1	Ebp2p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	EBP2	NP_012749.1
Pan troglodytes (chimpanzee)	EBNA1BP2	XP_003308087.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Smy2 and Syh1 may be involved in ribosome biogenesis in relation to Ebp2.
Title: SMY2 and SYH1 suppress defects in ribosome biogenesis caused by ebp2 mutations.

Depletion of either Ebp2 or Brx1 revealed that Brx1 requires Ebp2 for its stable association with pre-ribosomes, but Ebp2 does not depend on the presence of Brx1 to enter pre-ribosomes.
Title: Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae.

These results suggest that the ribosome biogenesis factors Ebp2 and Rrs1 cooperate with Mps3 to mediate telomere clustering, but not telomere tethering, by binding Sir4.
Title: Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres.

SUMO mediates the interaction between Ebp2p and SUMO related proteins and Ebp2p switches its interaction partners via sumoylation.
Title: SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1p.

Ebp2p is implicated in the secretory response, including changes in nucleolar architecture
Title: A ribosome assembly factor Ebp2p, the yeast homolog of EBNA1-binding protein 2, is involved in the secretory response.

The following EBP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02900	NM_001179738.1 Latest version!	Saccharomyces cerevisiae S288C Ebp2p (EBP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EBP2 ( NM_001179738.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012749.1 Saccharomyces cerevisiae S288C Ebp2p (EBP2), partial mRNA.

Price & Availability↑

CloneID	OSi02900
Clone ID Related Accession (Same CDS sequence)	NM_001179738.1
Accession Version	NM_001179738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ebp2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTAAAG GTTTCAAGTT GAAGGAGTTG CTTTCGCATC AAAAGGAAAT TGAAAAAGCT 
GAAAAGCTTG AAAATGACCT AAAGAAAAAG AAATCCCAAG AATTGAAGAA AGAAGAACCA 
ACTATCGTTA CAGCTTCTAA TTTGAAAAAG CTTGAGAAGA AAGAAAAGAA GGCCGATGTT 
AAGAAGGAAG TTGCTGCAGA TACTGAAGAA TATCAAAGCC AAGCCCTTTC TAAAAAAGAG 
AAAAGAAAGT TGAAAAAAGA ATTGAAAAAA ATGCAAGAAC AGGACGCTAC CGAAGCTCAA 
AAGCATATGT CTGGCGATGA AGATGAATCT GGCGACGATA GAGAGGAAGA AGAAGAAGAA 
GAAGAAGAAG AAGAAGGAAG ACTGGATCTT GAGAAATTGG CCAAAAGTGA CTCTGAGTCT 
GAAGATGATT CTGAGTCTGA AAATGATTCT GAAGAGGATG AGGATGTCGT TGCTAAAGAA 
GAAAGCGAGG AAAAGGAAGA GCAAGAAGAA GAACAAGACG TTCCATTGTC AGACGTGGAA 
TTTGATTCTG ATGCAGATGT CGTTCCACAT CATAAACTAA CCGTCAACAA CACAAAAGCG 
ATGAAACATG CCTTAGAAAG GGTACAATTG CCATGGAAAA AACATTCATT CCAAGAGCAC 
CAAAGCGTTA CATCAGAAAC TAATACGGAT GAACATATTA AGGACATATA CGATGATACT 
GAAAGGGAAT TAGCATTTTA CAAGCAATCA CTGGACGCTG TTTTAGTAGC ACGTGACGAA 
TTGAAACGAC TAAAAGTACC CTTCAAAAGA CCATTGGATT ATTTTGCTGA AATGGTGAAA 
AGTGATGAAC ATATGGACAA GATTAAGGGT AAGTTGATTG AAGAAGCCAG TGACAAGAAG 
GCACGTGAAG AAGCTAGAAG ACAAAGGCAA TTGAAGAAAT TTGGTAAGCA AGTCCAAAAC 
GCTACCTTAC AGAAACGTCA ATTGGAGAAA AGAGAAACAC TGGAAAAGAT CAAATCTTTG 
AAGAACAAAA GAAAGCACAA CGAGATCGAT CACTCCGAGT TCAACGTCGG TGTAGAGGAA 
GAAGTTGAAG GAAAAAGGTT CGATAGAGGC AGGCCTAACG GAAAGAGAGC CGCAAAAAAC 
GCTAAGTATG GCCAAGGAGG CATGAAGAGA TTCAAGAGGA AGAATGATGC TACCTCTTCT 
GCTGATGTCA GCGGCTTTTC TTCCAGAAAA ATGAAGGGGA AGACCAATAG ACCAGGAAAG 
AGTAGACGTG CCAGAAGATT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012749.1
CDS	1..1284
Translation	MAKGFKLKELLSHQKEIEKAEKLENDLKKKKSQELKKEEPTIVTASNLKKLEKKEKKADVKKEVAADTEEYQSQALSKKEKRKLKKELKKMQEQDATEAQKHMSGDEDESGDDREEEEEEEEEEEGRLDLEKLAKSDSESEDDSESENDSEEDEDVVAKEESEEKEEQEEEQDVPLSDVEFDSDADVVPHHKLTVNNTKAMKHALERVQLPWKKHSFQEHQSVTSETNTDEHIKDIYDDTERELAFYKQSLDAVLVARDELKRLKVPFKRPLDYFAEMVKSDEHMDKIKGKLIEEASDKKAREEARRQRQLKKFGKQVQNATLQKRQLEKRETLEKIKSLKNKRKHNEIDHSEFNVGVEEEVEGKRFDRGRPNGKRAAKNAKYGQGGMKRFKRKNDATSSADVSGFSSRKMKGKTNRPGKSRRARRF

Target ORF information:

RefSeq Version	NM_001179738.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ebp2p (EBP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179738.1

ORF Insert Sequence:

ATGGCTAAAG GTTTCAAGTT GAAGGAGTTG CTTTCGCATC AAAAGGAAAT TGAAAAAGCT 
GAAAAGCTTG AAAATGACCT AAAGAAAAAG AAATCCCAAG AATTGAAGAA AGAAGAACCA 
ACTATCGTTA CAGCTTCTAA TTTGAAAAAG CTTGAGAAGA AAGAAAAGAA GGCCGATGTT 
AAGAAGGAAG TTGCTGCAGA TACTGAAGAA TATCAAAGCC AAGCCCTTTC TAAAAAAGAG 
AAAAGAAAGT TGAAAAAAGA ATTGAAAAAA ATGCAAGAAC AGGACGCTAC CGAAGCTCAA 
AAGCATATGT CTGGCGATGA AGATGAATCT GGCGACGATA GAGAGGAAGA AGAAGAAGAA 
GAAGAAGAAG AAGAAGGAAG ACTGGATCTT GAGAAATTGG CCAAAAGTGA CTCTGAGTCT 
GAAGATGATT CTGAGTCTGA AAATGATTCT GAAGAGGATG AGGATGTCGT TGCTAAAGAA 
GAAAGCGAGG AAAAGGAAGA GCAAGAAGAA GAACAAGACG TTCCATTGTC AGACGTGGAA 
TTTGATTCTG ATGCAGATGT CGTTCCACAT CATAAACTAA CCGTCAACAA CACAAAAGCG 
ATGAAACATG CCTTAGAAAG GGTACAATTG CCATGGAAAA AACATTCATT CCAAGAGCAC 
CAAAGCGTTA CATCAGAAAC TAATACGGAT GAACATATTA AGGACATATA CGATGATACT 
GAAAGGGAAT TAGCATTTTA CAAGCAATCA CTGGACGCTG TTTTAGTAGC ACGTGACGAA 
TTGAAACGAC TAAAAGTACC CTTCAAAAGA CCATTGGATT ATTTTGCTGA AATGGTGAAA 
AGTGATGAAC ATATGGACAA GATTAAGGGT AAGTTGATTG AAGAAGCCAG TGACAAGAAG 
GCACGTGAAG AAGCTAGAAG ACAAAGGCAA TTGAAGAAAT TTGGTAAGCA AGTCCAAAAC 
GCTACCTTAC AGAAACGTCA ATTGGAGAAA AGAGAAACAC TGGAAAAGAT CAAATCTTTG 
AAGAACAAAA GAAAGCACAA CGAGATCGAT CACTCCGAGT TCAACGTCGG TGTAGAGGAA 
GAAGTTGAAG GAAAAAGGTT CGATAGAGGC AGGCCTAACG GAAAGAGAGC CGCAAAAAAC 
GCTAAGTATG GCCAAGGAGG CATGAAGAGA TTCAAGAGGA AGAATGATGC TACCTCTTCT 
GCTGATGTCA GCGGCTTTTC TTCCAGAAAA ATGAAGGGGA AGACCAATAG ACCAGGAAAG 
AGTAGACGTG CCAGAAGATT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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EBP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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