NSP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NSP1
Entrez Gene ID	853409
Full Name	FG-nucleoporin NSP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181474.3	NP_012494.3	FG-nucleoporin NSP1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	NSP1	NP_012494.3
Schizosaccharomyces pombe (fission yeast)	nsp1	NP_594158.1
Kluyveromyces lactis	KLLA0E11793g	XP_454482.1
Eremothecium gossypii	AGOS_ACR060W	NP_983463.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nsp1 releases a barrier that otherwise prevents nuclear pore complex passage through the bud neck to daughter cells.
Title: The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1.

Nic96 without its N domain binds in vitro to Phe-GLy repeats of the Nsp1-Nup49-Nup57 complex, suggesting how Nic96 could organize a transport module with coiled-coil domains and FG repeats in the central pore channel.
Title: Structural basis of the nic96 subcomplex organization in the nuclear pore channel.

findings show that phenylalanine-mediated inter-repeat interactions cross-link FG-repeat domains into elastic and reversible hydrogels; evidence obtained that such hydrogel formation is required for viability in yeast
Title: FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties.

The following NSP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02670	NM_001181474.3 Latest version!	Saccharomyces cerevisiae S288C FG-nucleoporin NSP1 (NSP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSP1 ( NM_001181474.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012494.3 Saccharomyces cerevisiae S288C FG-nucleoporin NSP1 (NSP1), partial mRNA.

Price & Availability↑

CloneID	OSi02670
Clone ID Related Accession (Same CDS sequence)	NM_001181474.3
Accession Version	NM_001181474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	FG-nucleoporin NSP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181474.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACTTCA ATACACCTCA ACAAAACAAA ACGCCCTTTT CGTTCGGGAC TGCCAACAAT 
AACTCTAACA CCACCAATCA GAATTCCTCT ACTGGTGCGG GCGCCTTCGG AACAGGTCAA 
TCAACATTTG GTTTCAACAA TTCTGCGCCA AATAACACGA ACAATGCAAA CTCTTCAATC 
ACACCTGCAT TTGGTAGCAA TAACACTGGT AATACTGCAT TCGGTAACAG TAACCCCACA 
AGTAACGTTT TTGGCAGCAA CAATTCTACG ACTAATACAT TTGGTAGTAA TAGTGCAGGG 
ACATCCCTTT TTGGATCTTC CAGTGCTCAA CAAACGAAGA GCAATGGAAC TGCTGGAGGA 
AATACATTTG GTTCTTCATC CTTATTCAAT AACAGTACTA ATAGCAATAC GACAAAACCT 
GCGTTCGGCG GTTTGAATTT TGGCGGCGGC AATAACACAA CTCCTAGCTC GACCGGTAAT 
GCTAACACAA GTAATAACTT ATTTGGCGCT ACCGCAAATG CGAATAAGCC AGCCTTTTCC 
TTTGGTGCAA CCACTAACGA CGATAAAAAA ACAGAACCAG ACAAGCCTGC TTTCTCCTTT 
AATTCAAGTG TCGGTAATAA GACAGACGCT CAAGCTCCCA CTACAGGATT TTCCTTTGGT 
TCACAACTAG GTGGAAACAA AACGGTTAAC GAGGCTGCAA AGCCTTCCCT TTCATTTGGT 
TCAGGATCAG CTGGTGCTAA CCCAGCTGGA GCTTCGCAAC CCGAACCTAC AACTAATGAA 
CCAGCAAAAC CAGCCCTCTC TTTTGGTACT GCAACATCCG ACAATAAGAC AACTAACACA 
ACCCCAAGTT TTTCGTTTGG TGCTAAATCG GATGAAAATA AAGCTGGTGC TACTTCTAAG 
CCTGCCTTCT CATTCGGTGC TAAGCCAGAA GAAAAGAAGG ATGATAATTC CTCAAAACCA 
GCTTTCTCAT TTGGTGCCAA ATCAAATGAA GACAAGCAAG ATGGAACGGC TAAGCCAGCA 
TTTTCCTTTG GTGCCAAGCC CGCGGAGAAG AATAACAATG AAACCTCCAA GCCGGCATTC 
TCTTTTGGAG CAAAGTCTGA TGAGAAAAAG GATGGTGATG CCTCTAAACC TGCCTTCTCT 
TTTGGCGCTA AACCGGATGA AAATAAAGCT AGTGCTACTT CTAAGCCTGC CTTCTCATTC 
GGCGCTAAGC CAGAAGAAAA GAAGGATGAT AATTCCTCAA AACCAGCTTT CTCATTTGGT 
GCCAAATCAA ATGAAGACAA GCAAGATGGA ACGGCTAAGC CAGCATTTTC CTTTGGTGCC 
AAGCCCGCGG AAAAGAATAA CAATGAAACT TCCAAGCCGG CATTCTCTTT TGGAGCAAAG 
TCCGATGAGA AAAAGGATGG CGATGCCTCT AAACCTGCCT TCTCATTTGG TGCTAAATCG 
GATGAAAAAA AGGACAGTGA TTCTTCGAAG CCTGCCTTCT CATTTGGTAC TAAATCGAAT 
GAAAAAAAGG ACAGTGGTTC TTCGAAGCCT GCCTTCTCAT TCGGTGCTAA ACCCGATGAA 
AAGAAGAATG ATGAAGTCTC CAAGCCTGCC TTCTCATTCG GAGCAAAGGC TAACGAAAAG 
AAAGAAAGTG ATGAATCAAA ATCAGCCTTT TCATTTGGTT CTAAGCCTAC AGGAAAGGAG 
GAAGGCGATG GGGCGAAGGC TGCCATTTCC TTTGGTGCTA AACCCGAAGA ACAGAAATCC 
AGTGATACGA GCAAGCCAGC GTTTACTTTT GGTGCTCAAA AAGATAATGA GAAGAAAACA 
GAGGAATCTT CTACGGGGAA GTCAACCGCA GATGTCAAAT CAAGTGATAG CTTGAAATTA 
AATTCTAAAC CAGTAGAATT GAAGCCAGTT TCTCTCGACA ATAAAACACT GGACGATTTA 
GTAACGAAAT GGACTAACCA ACTAACAGAA TCGGCCTCTC ATTTTGAACA GTATACCAAA 
AAAATTAATA GTTGGGATCA GGTACTAGTG AAAGGAGGTG AACAAATTAG TCAGTTATAC 
TCAGATGCTG TGATGGCGGA ACATAGTCAA AACAAAATAG ATCAATCTCT ACAATATATC 
GAGAGGCAAC AGGATGAATT AGAAAACTTC TTAGACAATT TTGAAACCAA GACAGAGGCA 
CTTCTGTCCG ATGTTGTGTC TACAAGTTCA GGTGCTGCGG CCAACAATAA TGACCAAAAA 
AGACAACAGG CCTATAAGAC AGCCCAGACT TTGGACGAAA ATTTAAACTC GTTGTCATCC 
AATCTTTCTT CTCTAATAGT AGAAATCAAC AATGTTAGTA ATACATTCAA CAAAACCACT 
AACATCGATA TAAACAATGA AGACGAAAAT ATTCAACTAA TCAAAATCTT GAATTCACAT 
TTCGACGCTT TAAGGTCACT AGACGATAAC TCCACCTCTC TGGAAAAACA AATCAACTCG 
ATAAAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012494.3
CDS	1..2472
Translation	MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFGSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSHFDALRSLDDNSTSLEKQINSIKK

Target ORF information:

RefSeq Version	NM_001181474.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C FG-nucleoporin NSP1 (NSP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181474.3

ORF Insert Sequence:

ATGAACTTCA ATACACCTCA ACAAAACAAA ACGCCCTTTT CGTTCGGGAC TGCCAACAAT 
AACTCTAACA CCACCAATCA GAATTCCTCT ACTGGTGCGG GCGCCTTCGG AACAGGTCAA 
TCAACATTTG GTTTCAACAA TTCTGCGCCA AATAACACGA ACAATGCAAA CTCTTCAATC 
ACACCTGCAT TTGGTAGCAA TAACACTGGT AATACTGCAT TCGGTAACAG TAACCCCACA 
AGTAACGTTT TTGGCAGCAA CAATTCTACG ACTAATACAT TTGGTAGTAA TAGTGCAGGG 
ACATCCCTTT TTGGATCTTC CAGTGCTCAA CAAACGAAGA GCAATGGAAC TGCTGGAGGA 
AATACATTTG GTTCTTCATC CTTATTCAAT AACAGTACTA ATAGCAATAC GACAAAACCT 
GCGTTCGGCG GTTTGAATTT TGGCGGCGGC AATAACACAA CTCCTAGCTC GACCGGTAAT 
GCTAACACAA GTAATAACTT ATTTGGCGCT ACCGCAAATG CGAATAAGCC AGCCTTTTCC 
TTTGGTGCAA CCACTAACGA CGATAAAAAA ACAGAACCAG ACAAGCCTGC TTTCTCCTTT 
AATTCAAGTG TCGGTAATAA GACAGACGCT CAAGCTCCCA CTACAGGATT TTCCTTTGGT 
TCACAACTAG GTGGAAACAA AACGGTTAAC GAGGCTGCAA AGCCTTCCCT TTCATTTGGT 
TCAGGATCAG CTGGTGCTAA CCCAGCTGGA GCTTCGCAAC CCGAACCTAC AACTAATGAA 
CCAGCAAAAC CAGCCCTCTC TTTTGGTACT GCAACATCCG ACAATAAGAC AACTAACACA 
ACCCCAAGTT TTTCGTTTGG TGCTAAATCG GATGAAAATA AAGCTGGTGC TACTTCTAAG 
CCTGCCTTCT CATTCGGTGC TAAGCCAGAA GAAAAGAAGG ATGATAATTC CTCAAAACCA 
GCTTTCTCAT TTGGTGCCAA ATCAAATGAA GACAAGCAAG ATGGAACGGC TAAGCCAGCA 
TTTTCCTTTG GTGCCAAGCC CGCGGAGAAG AATAACAATG AAACCTCCAA GCCGGCATTC 
TCTTTTGGAG CAAAGTCTGA TGAGAAAAAG GATGGTGATG CCTCTAAACC TGCCTTCTCT 
TTTGGCGCTA AACCGGATGA AAATAAAGCT AGTGCTACTT CTAAGCCTGC CTTCTCATTC 
GGCGCTAAGC CAGAAGAAAA GAAGGATGAT AATTCCTCAA AACCAGCTTT CTCATTTGGT 
GCCAAATCAA ATGAAGACAA GCAAGATGGA ACGGCTAAGC CAGCATTTTC CTTTGGTGCC 
AAGCCCGCGG AAAAGAATAA CAATGAAACT TCCAAGCCGG CATTCTCTTT TGGAGCAAAG 
TCCGATGAGA AAAAGGATGG CGATGCCTCT AAACCTGCCT TCTCATTTGG TGCTAAATCG 
GATGAAAAAA AGGACAGTGA TTCTTCGAAG CCTGCCTTCT CATTTGGTAC TAAATCGAAT 
GAAAAAAAGG ACAGTGGTTC TTCGAAGCCT GCCTTCTCAT TCGGTGCTAA ACCCGATGAA 
AAGAAGAATG ATGAAGTCTC CAAGCCTGCC TTCTCATTCG GAGCAAAGGC TAACGAAAAG 
AAAGAAAGTG ATGAATCAAA ATCAGCCTTT TCATTTGGTT CTAAGCCTAC AGGAAAGGAG 
GAAGGCGATG GGGCGAAGGC TGCCATTTCC TTTGGTGCTA AACCCGAAGA ACAGAAATCC 
AGTGATACGA GCAAGCCAGC GTTTACTTTT GGTGCTCAAA AAGATAATGA GAAGAAAACA 
GAGGAATCTT CTACGGGGAA GTCAACCGCA GATGTCAAAT CAAGTGATAG CTTGAAATTA 
AATTCTAAAC CAGTAGAATT GAAGCCAGTT TCTCTCGACA ATAAAACACT GGACGATTTA 
GTAACGAAAT GGACTAACCA ACTAACAGAA TCGGCCTCTC ATTTTGAACA GTATACCAAA 
AAAATTAATA GTTGGGATCA GGTACTAGTG AAAGGAGGTG AACAAATTAG TCAGTTATAC 
TCAGATGCTG TGATGGCGGA ACATAGTCAA AACAAAATAG ATCAATCTCT ACAATATATC 
GAGAGGCAAC AGGATGAATT AGAAAACTTC TTAGACAATT TTGAAACCAA GACAGAGGCA 
CTTCTGTCCG ATGTTGTGTC TACAAGTTCA GGTGCTGCGG CCAACAATAA TGACCAAAAA 
AGACAACAGG CCTATAAGAC AGCCCAGACT TTGGACGAAA ATTTAAACTC GTTGTCATCC 
AATCTTTCTT CTCTAATAGT AGAAATCAAC AATGTTAGTA ATACATTCAA CAAAACCACT 
AACATCGATA TAAACAATGA AGACGAAAAT ATTCAACTAA TCAAAATCTT GAATTCACAT 
TTCGACGCTT TAAGGTCACT AGACGATAAC TCCACCTCTC TGGAAAAACA AATCAACTCG 
ATAAAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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NSP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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