RTN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RTN1
Entrez Gene ID	851819
Full Name	Rtn1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180541.3	NP_010519.3	Rtn1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABL105W	NP_982842.1
Kluyveromyces lactis	KLLA0C15081g	XP_452876.1
Saccharomyces cerevisiae (baker's yeast)	RTN1	NP_010519.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two integral membrane peroxins (proteins required for peroxisome biogenesis) in Saccharomyces cerevisiae, Pex29 and Pex30, reside in distinct regions of the ER and associate with Rtn1 and Yop1, reticulon family members that contribute to ER morphology, to govern peroxisome emergence from the ER.
Title: Peroxins Pex30 and Pex29 Dynamically Associate with Reticulons to Regulate Peroxisome Biogenesis from the Endoplasmic Reticulum.

Rtn1 and Yop1 also physically interacted with Ndc1 and other NPC membrane proteins. We propose that NPC and SPB biogenesis are altered in cells lacking Rtn1 and Yop1 due to competition between these complexes for Ndc1
Title: Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1.

reveal and dissect the critical roles of the reticulon homology domain proteins (Rtn1p, Rtn2p, and Yop1p) in brome mosaic virus RNA replication compartment formation and function
Title: Membrane-shaping host reticulon proteins play crucial roles in viral RNA replication compartment formation and function.

In this study, we report that members of the RTN and Yop1/DP1 families are required for nuclear pore formation.
Title: ER membrane-bending proteins are necessary for de novo nuclear pore formation.

Sbh1p co-precipitates with the ER transmembrane protein Rtn1p. Sbh1p-Rtn1p complexes do not appear to contain Sss1p and Sec61p.
Title: The transmembrane domain is sufficient for Sbh1p function, its association with the Sec61 complex, and interaction with Rtn1p.

The following RTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01313	NM_001180541.3 Latest version!	Saccharomyces cerevisiae S288C Rtn1p (RTN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTN1 ( NM_001180541.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010519.3 Saccharomyces cerevisiae S288C Rtn1p (RTN1), partial mRNA.

Price & Availability↑

CloneID	OSi01313
Clone ID Related Accession (Same CDS sequence)	NM_001180541.3
Accession Version	NM_001180541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rtn1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180541.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCGCCT CAGCTCAACA TAGCCAAGCC CAACAACAAC AACAACAAAA AAGCTGTAAT 
TGCGATTTGC TATTATGGAG GAACCCTGTT CAAACCGGTA AATATTTCGG TGGGTCTCTG 
TTAGCCTTGT TAATTTTGAA AAAAGTTAAC TTGATCACTT TCTTTTTGAA GGTTGCCTAC 
ACTATCTTAT TCACAACAGG ATCTATTGAA TTCGTATCCA AGCTCTTTTT GGGACAAGGT 
TTAATCACCA AGTATGGTCC TAAAGAATGT CCAAATATCG CCGGCTTTAT TAAGCCTCAT 
ATCGATGAAG CTTTGAAACA GCTACCAGTC TTCCAAGCCC ACATAAGAAA GACTGTATTC 
GCTCAAGTCC CTAAGCACAC TTTCAAGACC GCTGTTGCCT TATTTCTACT ACACAAATTC 
TTCTCCTGGT TCTCTATCTG GACCATCGTC TTTGTTGCTG ACATTTTTAC CTTCACTTTG 
CCAGTTATCT ACCATTCCTA CAAGCATGAA ATTGATGCTA CTGTCGCTCA AGGTGTAGAA 
ATTTCTAAGC AAAAGACCCA AGAGTTTTCT CAAATGGCTT GCGAAAAGAC AAAACCATAC 
TTGGACAAGG TTGAATCTAA ATTGGGCCCA ATTTCCAACT TGGTCAAATC TAAGACTGCT 
CCTGTGTCTT CTACTGCCGG TCCTCAAACC GCCAGCACTT CTAAGTTGGC CGCTGATGTT 
CCATTGGAAC CAGAGTCTAA GGCTTACACA TCTTCAGCTC AAGTTATGCC AGAAGTTCCA 
CAACACGAAC CTTCCACTAC CCAAGAATTT AACGTCGATG AATTGTCTAA TGAATTGAAG 
AAAAGTACAA AAAACTTGCA AAATGAATTG GAAAAAAACA ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010519.3
CDS	1..888
Translation	MSASAQHSQAQQQQQQKSCNCDLLLWRNPVQTGKYFGGSLLALLILKKVNLITFFLKVAYTILFTTGSIEFVSKLFLGQGLITKYGPKECPNIAGFIKPHIDEALKQLPVFQAHIRKTVFAQVPKHTFKTAVALFLLHKFFSWFSIWTIVFVADIFTFTLPVIYHSYKHEIDATVAQGVEISKQKTQEFSQMACEKTKPYLDKVESKLGPISNLVKSKTAPVSSTAGPQTASTSKLAADVPLEPESKAYTSSAQVMPEVPQHEPSTTQEFNVDELSNELKKSTKNLQNELEKNNA

Target ORF information:

RefSeq Version	NM_001180541.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rtn1p (RTN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180541.3

ORF Insert Sequence:

ATGTCCGCCT CAGCTCAACA TAGCCAAGCC CAACAACAAC AACAACAAAA AAGCTGTAAT 
TGCGATTTGC TATTATGGAG GAACCCTGTT CAAACCGGTA AATATTTCGG TGGGTCTCTG 
TTAGCCTTGT TAATTTTGAA AAAAGTTAAC TTGATCACTT TCTTTTTGAA GGTTGCCTAC 
ACTATCTTAT TCACAACAGG ATCTATTGAA TTCGTATCCA AGCTCTTTTT GGGACAAGGT 
TTAATCACCA AGTATGGTCC TAAAGAATGT CCAAATATCG CCGGCTTTAT TAAGCCTCAT 
ATCGATGAAG CTTTGAAACA GCTACCAGTC TTCCAAGCCC ACATAAGAAA GACTGTATTC 
GCTCAAGTCC CTAAGCACAC TTTCAAGACC GCTGTTGCCT TATTTCTACT ACACAAATTC 
TTCTCCTGGT TCTCTATCTG GACCATCGTC TTTGTTGCTG ACATTTTTAC CTTCACTTTG 
CCAGTTATCT ACCATTCCTA CAAGCATGAA ATTGATGCTA CTGTCGCTCA AGGTGTAGAA 
ATTTCTAAGC AAAAGACCCA AGAGTTTTCT CAAATGGCTT GCGAAAAGAC AAAACCATAC 
TTGGACAAGG TTGAATCTAA ATTGGGCCCA ATTTCCAACT TGGTCAAATC TAAGACTGCT 
CCTGTGTCTT CTACTGCCGG TCCTCAAACC GCCAGCACTT CTAAGTTGGC CGCTGATGTT 
CCATTGGAAC CAGAGTCTAA GGCTTACACA TCTTCAGCTC AAGTTATGCC AGAAGTTCCA 
CAACACGAAC CTTCCACTAC CCAAGAATTT AACGTCGATG AATTGTCTAA TGAATTGAAG 
AAAAGTACAA AAAACTTGCA AAATGAATTG GAAAAAAACA ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RTN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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