UME6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UME6
Entrez Gene ID	851788
Full Name	DNA-binding transcriptional regulator UME6
Synonyms	CAR80,NIM2,RIM16
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180515.1	NP_010493.1	DNA-binding transcriptional regulator UME6

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We also present evidence that indicates Rim11/Ume6 works independently but in parallel with that of checkpoint inhibition, dNTP regulation, and sister-chromatid recombination. In conclusion, our results establish Rim11, Ume6, the histone deacetylase complex Sin3-Rpd3 and Sml1 as new factors important in the events of faulty lagging strand synthesis.
Title: GSK-3? Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2.

identifies novel Ume6 repressed genes during growth and development and reveals a strong effect of the carbon source on the derepression pattern of transcripts in growing and developmentally arrested ume6/ume6 mutant cells
Title: Global alterations of the transcriptional landscape during yeast growth and development in the absence of Ume6-dependent chromatin modification.

evidence that the Rpd3/Sin3/Ume6 histone deacetylase complex, which represses meiotic genes during mitosis, also prevents the induction of BOI1's 5'-extended isoform in mitosis by direct binding of Ume6 to its URS1 target
Title: The conserved histone deacetylase Rpd3 and the DNA binding regulator Ume6 repress BOI1's meiotic transcript isoform during vegetative growth in Saccharomyces cerevisiae.

acetylation inhibits Ume6p binding by electrostatic repulsion
Title: Acetylation of the transcriptional repressor Ume6p allows efficient promoter release and timely induction of the meiotic transient transcription program in yeast.

Ume6 is a negative regulator of ATG8 transcription, which acts along with a histone deacetylase complex including Sin3 and Rpd3 to regulate Atg8 levels
Title: Ume6 transcription factor is part of a signaling cascade that regulates autophagy.

The following UME6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UME6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01287	NM_001180515.1 Latest version!	Saccharomyces cerevisiae S288C DNA-binding transcriptional regulator UME6 (UME6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UME6 ( NM_001180515.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010493.1 Saccharomyces cerevisiae S288C DNA-binding transcriptional regulator UME6 (UME6), partial mRNA.

Price & Availability↑

CloneID	OSi01287
Clone ID Related Accession (Same CDS sequence)	NM_001180515.1
Accession Version	NM_001180515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-binding transcriptional regulator UME6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTAGACA AGGCGCGCTC TCAAAGCAAA CACATGGACG AATCTAATGC GGCTGCCTCT 
CTGCTTTCGA TGGAAACAAC CGCCAACAAT CATCACTATT TGCACAATAA AACATCTCGT 
GCCACGCTGA TGAATAGCAG CCAAGACGGC AAAAAACATG CAGAAGATGA AGTTAGTGAT 
GGAGCTAACT CCCGCCATCC TACAATTTCC AGTGCTAGCA TCGAATCTCT CAAGACAACC 
TACGATGAAA ACCCTTTGCT TTCTATTATG AAATCGACAT GTGCGCCCAA CAACACTCCC 
GTGCATACTC CGTCTGGTTC GCCGAGTTTG AAAGTCCAAA GTGGCGGAGA TATCAAAGAC 
GATCCTAAGG AAAACGATAC TACTACTACG ACCAATACTA CACTTCAAGA TCGCCGTGAC 
AGCGATAACG CTGTGCATGC TGCCGCCAGC CCACTCGCGC CTTCCAATAC ACCTTCGGAT 
CCTAAGTCAT TGTGCAATGG CCATGTCGCA CAGGCTACAG ACCCACAAAT TTCCGGTGCT 
ATTCAGCCGC AGTATACTGC AACCAACGAG GATGTTTTCC CTTACTCCTC CACCTCCACT 
AATAGTAACA CTGCCACTAC TACTATCGTC GCCGGCGCCA AAAAAAAAAT ACATTTGCCG 
CCACCACAAG CTCCAGCGGT TTCTTCCCCC GGTACCACCG CAGCAGGCTC GGGCGCGGGC 
ACGGGCTCGG GCATCCGTTC CCGCACAGGA TCGGATTTGC CGCTCATCAT TACCAGCGCC 
AACAAGAACA ACGGTAAGAC TACCAATTCG CCTATGTCGA TACTGAGCAG AAACAACAGT 
ACCAACAACA ACGACAATAA TTCGATACAA AGCTCAGATT CGAGAGAATC CTCTAATAAC 
AATGAGATTG GCGGGTATTT GCGCGGCGGA ACTAAGCGCG GCGGCAGTCC ATCTAACGAC 
TCTCAGGTCC AGCATAATGT GCATGATGAC CAATGTGCCG TGGGCGTGGC GCCCAGGAAC 
TTCTATTTCA ACAAGGATAG AGAGATAACA GACCCAAATG TAAAACTGGA CGAGAACGAA 
TCAAAAATCA ACATATCGTT CTGGCTAAAT TCGAAATACA GAGATGAGGC TTATTCTTTG 
AATGAATCAT CCTCCAACAA TGCTAGTTCA AACACGGATA CGCCTACAAA CTCTCGACAT 
GCGAACACCA GCTCTTCCAT TACCAGCAGA AACAATTTCC AGCATTTTAG GTTCAACCAA 
ATACCTTCTC AACCTCCAAC TTCCGCATCT TCGTTTACAA GCACAAACAA CAACAACCCT 
CAACGGAACA ATATCAATCG CGGTGAAGAC CCGTTTGCCA CTTCGTCAAG ACCTTCTACT 
GGGTTTTTTT ACGGCGATTT GCCGAATCGT AACAATAGAA ATAGTCCCTT CCATACAAAT 
GAACAATACA TCCCACCACC TCCACCGAAA TACATCAATT CTAAATTGGA TGGATTGAGA 
TCAAGATTAT TGCTCGGTCC GAATTCTGCA TCTTCATCTA CCAAACTAGA CGACGACTTG 
GGTACAGCAG CAGCAGTGCT ATCAAACATG AGATCATCCC CATATAGAAC TCATGATAAA 
CCCATTTCCA ATGTCAATGA CATGAATAAC ACAAATGCGC TCGGTGTGCC GGCTAGTAGG 
CCTCATTCGT CATCTTTTCC ATCAAAGGGT GTCTTAAGAC CAATTCTGTT ACGTATCCAT 
AATTCCGAAC AACAACCCAT TTTCGAAAGC AACAATTCTA CAGCTGTTTT TGATGAAGAC 
CAGGACCAAA ATCAAGACTT GTCTCCTTAC CATTTAAATC TAAACTCTAA AAAGGTTTTA 
GATCCCACTT TTGAGTCAAG GACAAGGCAA GTTACTTGGA ATAAGAATGG TAAGCGAATA 
GACAGACGCC TTTCTGCTCC AGAACAACAA CAGCAACTGG AAGTTCCACC ATTGAAAAAA 
TCGAGAAGGT CAGTTGGAAA CGCAAGAGTA GCAAGCCAAA CCAATAGCGA TTATAATTCT 
CTTGGCGAAT CCTCAACTTC GTCAGCTCCA TCGTCTCCAT CTTTGAAGGC TTCTTCTGGC 
TTGGCATATA CCGCTGATTA TCCTAACGCT ACTTCGCCGG ATTTCGCTAA ATCTAAAGGA 
AAAAATGTCA AGCCTAAGGC AAAATCAAAG GCGAAACAGT CATCAAAGAA AAGACCAAAT 
AATACTACTT CGAAATCAAA AGCAAACAAT TCTCAAGAAT CGAATAATGC TACTTCCTCA 
ACGTCTCAAG GTACAAGGTC CCGTACTGGT TGCTGGATTT GTAGATTAAG GAAAAAGAAG 
TGTACCGAGG AAAGACCGCA CTGTTTCAAC TGTGAAAGGT TGAAATTGGA CTGTCACTAT 
GACGCGTTCA AACCAGATTT TGTATCTGAT CCAAAGAAAA AACAGATGAA ACTGGAGGAA 
ATCAAGAAAA AAACAAAAGA GGCCAAAAGA AGAGCAATGA AAAAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010493.1
CDS	1..2511
Translation	MLDKARSQSKHMDESNAAASLLSMETTANNHHYLHNKTSRATLMNSSQDGKKHAEDEVSDGANSRHPTISSASIESLKTTYDENPLLSIMKSTCAPNNTPVHTPSGSPSLKVQSGGDIKDDPKENDTTTTTNTTLQDRRDSDNAVHAAASPLAPSNTPSDPKSLCNGHVAQATDPQISGAIQPQYTATNEDVFPYSSTSTNSNTATTTIVAGAKKKIHLPPPQAPAVSSPGTTAAGSGAGTGSGIRSRTGSDLPLIITSANKNNGKTTNSPMSILSRNNSTNNNDNNSIQSSDSRESSNNNEIGGYLRGGTKRGGSPSNDSQVQHNVHDDQCAVGVAPRNFYFNKDREITDPNVKLDENESKINISFWLNSKYRDEAYSLNESSSNNASSNTDTPTNSRHANTSSSITSRNNFQHFRFNQIPSQPPTSASSFTSTNNNNPQRNNINRGEDPFATSSRPSTGFFYGDLPNRNNRNSPFHTNEQYIPPPPPKYINSKLDGLRSRLLLGPNSASSSTKLDDDLGTAAAVLSNMRSSPYRTHDKPISNVNDMNNTNALGVPASRPHSSSFPSKGVLRPILLRIHNSEQQPIFESNNSTAVFDEDQDQNQDLSPYHLNLNSKKVLDPTFESRTRQVTWNKNGKRIDRRLSAPEQQQQLEVPPLKKSRRSVGNARVASQTNSDYNSLGESSTSSAPSSPSLKASSGLAYTADYPNATSPDFAKSKGKNVKPKAKSKAKQSSKKRPNNTTSKSKANNSQESNNATSSTSQGTRSRTGCWICRLRKKKCTEERPHCFNCERLKLDCHYDAFKPDFVSDPKKKQMKLEEIKKKTKEAKRRAMKKK

Target ORF information:

RefSeq Version	NM_001180515.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-binding transcriptional regulator UME6 (UME6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180515.1

ORF Insert Sequence:

ATGCTAGACA AGGCGCGCTC TCAAAGCAAA CACATGGACG AATCTAATGC GGCTGCCTCT 
CTGCTTTCGA TGGAAACAAC CGCCAACAAT CATCACTATT TGCACAATAA AACATCTCGT 
GCCACGCTGA TGAATAGCAG CCAAGACGGC AAAAAACATG CAGAAGATGA AGTTAGTGAT 
GGAGCTAACT CCCGCCATCC TACAATTTCC AGTGCTAGCA TCGAATCTCT CAAGACAACC 
TACGATGAAA ACCCTTTGCT TTCTATTATG AAATCGACAT GTGCGCCCAA CAACACTCCC 
GTGCATACTC CGTCTGGTTC GCCGAGTTTG AAAGTCCAAA GTGGCGGAGA TATCAAAGAC 
GATCCTAAGG AAAACGATAC TACTACTACG ACCAATACTA CACTTCAAGA TCGCCGTGAC 
AGCGATAACG CTGTGCATGC TGCCGCCAGC CCACTCGCGC CTTCCAATAC ACCTTCGGAT 
CCTAAGTCAT TGTGCAATGG CCATGTCGCA CAGGCTACAG ACCCACAAAT TTCCGGTGCT 
ATTCAGCCGC AGTATACTGC AACCAACGAG GATGTTTTCC CTTACTCCTC CACCTCCACT 
AATAGTAACA CTGCCACTAC TACTATCGTC GCCGGCGCCA AAAAAAAAAT ACATTTGCCG 
CCACCACAAG CTCCAGCGGT TTCTTCCCCC GGTACCACCG CAGCAGGCTC GGGCGCGGGC 
ACGGGCTCGG GCATCCGTTC CCGCACAGGA TCGGATTTGC CGCTCATCAT TACCAGCGCC 
AACAAGAACA ACGGTAAGAC TACCAATTCG CCTATGTCGA TACTGAGCAG AAACAACAGT 
ACCAACAACA ACGACAATAA TTCGATACAA AGCTCAGATT CGAGAGAATC CTCTAATAAC 
AATGAGATTG GCGGGTATTT GCGCGGCGGA ACTAAGCGCG GCGGCAGTCC ATCTAACGAC 
TCTCAGGTCC AGCATAATGT GCATGATGAC CAATGTGCCG TGGGCGTGGC GCCCAGGAAC 
TTCTATTTCA ACAAGGATAG AGAGATAACA GACCCAAATG TAAAACTGGA CGAGAACGAA 
TCAAAAATCA ACATATCGTT CTGGCTAAAT TCGAAATACA GAGATGAGGC TTATTCTTTG 
AATGAATCAT CCTCCAACAA TGCTAGTTCA AACACGGATA CGCCTACAAA CTCTCGACAT 
GCGAACACCA GCTCTTCCAT TACCAGCAGA AACAATTTCC AGCATTTTAG GTTCAACCAA 
ATACCTTCTC AACCTCCAAC TTCCGCATCT TCGTTTACAA GCACAAACAA CAACAACCCT 
CAACGGAACA ATATCAATCG CGGTGAAGAC CCGTTTGCCA CTTCGTCAAG ACCTTCTACT 
GGGTTTTTTT ACGGCGATTT GCCGAATCGT AACAATAGAA ATAGTCCCTT CCATACAAAT 
GAACAATACA TCCCACCACC TCCACCGAAA TACATCAATT CTAAATTGGA TGGATTGAGA 
TCAAGATTAT TGCTCGGTCC GAATTCTGCA TCTTCATCTA CCAAACTAGA CGACGACTTG 
GGTACAGCAG CAGCAGTGCT ATCAAACATG AGATCATCCC CATATAGAAC TCATGATAAA 
CCCATTTCCA ATGTCAATGA CATGAATAAC ACAAATGCGC TCGGTGTGCC GGCTAGTAGG 
CCTCATTCGT CATCTTTTCC ATCAAAGGGT GTCTTAAGAC CAATTCTGTT ACGTATCCAT 
AATTCCGAAC AACAACCCAT TTTCGAAAGC AACAATTCTA CAGCTGTTTT TGATGAAGAC 
CAGGACCAAA ATCAAGACTT GTCTCCTTAC CATTTAAATC TAAACTCTAA AAAGGTTTTA 
GATCCCACTT TTGAGTCAAG GACAAGGCAA GTTACTTGGA ATAAGAATGG TAAGCGAATA 
GACAGACGCC TTTCTGCTCC AGAACAACAA CAGCAACTGG AAGTTCCACC ATTGAAAAAA 
TCGAGAAGGT CAGTTGGAAA CGCAAGAGTA GCAAGCCAAA CCAATAGCGA TTATAATTCT 
CTTGGCGAAT CCTCAACTTC GTCAGCTCCA TCGTCTCCAT CTTTGAAGGC TTCTTCTGGC 
TTGGCATATA CCGCTGATTA TCCTAACGCT ACTTCGCCGG ATTTCGCTAA ATCTAAAGGA 
AAAAATGTCA AGCCTAAGGC AAAATCAAAG GCGAAACAGT CATCAAAGAA AAGACCAAAT 
AATACTACTT CGAAATCAAA AGCAAACAAT TCTCAAGAAT CGAATAATGC TACTTCCTCA 
ACGTCTCAAG GTACAAGGTC CCGTACTGGT TGCTGGATTT GTAGATTAAG GAAAAAGAAG 
TGTACCGAGG AAAGACCGCA CTGTTTCAAC TGTGAAAGGT TGAAATTGGA CTGTCACTAT 
GACGCGTTCA AACCAGATTT TGTATCTGAT CCAAAGAAAA AACAGATGAA ACTGGAGGAA 
ATCAAGAAAA AAACAAAAGA GGCCAAAAGA AGAGCAATGA AAAAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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UME6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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