AHK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	AHK1
Entrez Gene ID	851486
Full Name	Ahk1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180132.1	NP_010210.1	Ahk1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B03003g	XP_451666.1
Eremothecium gossypii	AGOS_AER073C	NP_984933.2
Saccharomyces cerevisiae (baker's yeast)	YDL073W	NP_010210.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ahk1 is a scaffold protein in the HKR1 subbranch and prevents incorrect signal flow from Hkr1 to Kss1
Title: Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase.

The following AHK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AHK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01032	NM_001180132.1 Latest version!	Saccharomyces cerevisiae S288C Ahk1p (AHK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AHK1 ( NM_001180132.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010210.1 Saccharomyces cerevisiae S288C Ahk1p (AHK1), partial mRNA.

Price & Availability↑

CloneID	OSi01032
Clone ID Related Accession (Same CDS sequence)	NM_001180132.1
Accession Version	NM_001180132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ahk1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTCCTG CCATACATAA TCAACTATAT GAATGTGGAT TAAAGACTAC GTCTCAAGAT 
GGCTTCTTGA TGGACAATTA TGAAACTCAA TTATTTCAAT CGTTGAACAG GTTTATCAAT 
TTCATAAACA ATGCTAATCA GTCAGCTAGT AATAAAGAAC AAAATACAAA ATTTTGCAAA 
TCCTCCTCAA ATTTTTTACG CTTGAAACTA CTAACGGTTC TAAGACAATG GTGTGATTCT 
TCACGAAGCA ACTGTACACT GGAGGTTAGA GATGTTTTGA CCCAATGGTG GGTCACACTT 
TTAAACTTTC TAAATTCTGA CACCTCGCTT CAAATAGATA CTGCCCTTGA ATTGAGCTTG 
AGCATAGAAC TAACTTCCGT GTGTCTCGAG TGCCTCAGCA AAATTATGAC CATACTAATA 
ATATTGCCTT TCCATTCATC CAGAGACATG GAAATATACT CACATCATCT TTTGCTGACC 
ATTCATTGCA TAACAAACAA GTTGATTTTG ATTTCTAAAA ACTCCAAAAA ACTTAAACGT 
ACAAATAGTG ATGACAAGTG CTCTATAAAC GACAAGAAGC TGCAATACCT TAACAAATAT 
TCTTCCTTGC TGCGAGCATT CATCGGGAAG TTAAATGCTT ACGCATTTTT TTATCTACCA 
GAAGACTTTC ATTTCGATAC AATTTTGTTA TTGACAGTTT CTCCTCAAAT CTCTTCAAGT 
ATTCAAACAT CTTTGTTTTC ATGGAAGAAG AGACAATATA AATTCACCGA CGATCAGGGA 
CAAATGATCC GCACTGAAGC TTTTGAAAAT AAAGATACTA AATTTTTTAA GATCATTGTT 
TCTTATATTA AAAATGATTT TGTACTTATG TCATTTTATT GGCACTATTG GTATATCATA 
CTTCAATTTA TGAAATTCTC TGATTCAGAT GTAGGAATTA AAAAATCGAC ATTGTCATGC 
ATACCTGGCT CAGAAATTTT ATTGACGCAT GTAACCACTA GATTCTTGAA TTCAGATTTG 
AATAAATTCA CGCGGATTAT TAAACAAACG CCGAATCCAA GGATAGCAAA TGAGAATGTG 
ACAGAGTCAC ATCCAAATTT CAAGAGTTTG AATAGTAGCA ATGCTTTAAT TACATCTGAA 
AGGATTAATG ATTACGTATT TTCTAATTTT AAGACAATCA AATTATGGGA ATGTCTTAGA 
AGCCTTTCAG GATGCATTCT TAAAGAAAAT CATCCGGAAT ACCTGGAAAA TATGCTTTCC 
TTACATGAGT CTCTATTGAT AGACTATGTC TCCACTATTT CAGCGTACGA TTATATTGCA 
GCGAACGTCA TTTACAATAA GGTTCTCCAG TTTATTATTT TCCAGTTTGA AAGTTTACCT 
TCTCTGAAAT TCATTCAATG GAGGTCGTGG TACAATGGGC TTTTGAGTAT GTTAAGGACC 
AAAAATGTTA ACTGCCAGAC TGTTTCTTTA TTATGCCTTT TCAATATTTG GAAGCACGTA 
ACCATTGAGG ACCGCGATGA AATAGTAAAA GTGCTGTTAA GTGATTTTTG GGAGTCATTG 
ATTTTTGAAA ACGAATTTCC TTTGATTAGA ATTTTATTTA TGAAGGTATT AGTTTTTAAA 
ATCATTCCAA GTGTTCAAAA TTCTTCTTCA TTACGATTCT TGCCACATGA TAGAATTAAA 
CAACTATATG AAGAGCTACT GGTAAACAAG GAGGAGTTAT TTGAGATGCA AAAACACGAT 
TCAAATGATA TAGTGGCGCA TAGAAAGAAT GCTCTCGTTT TTAATGGTAA CTCACGTTTG 
ATGATGATTC CAAAAAAACC AAATACAGAG GACCACTTAG TTTATAAGAT TAATCATGAT 
AAAAATTTAA CTACAGAAAG ATTTCCCTCT GTCAGCTCAG TGGCAAATAC AAGACCTAAC 
GTTATCTTGA AAAATGGTAA ATACGCTTAT GATATTTTGG ACGAAATGAC AAGTAAAGCT 
GCCTTTCTTC TTGCAGAAAA GAAGACCCGA CTAAATCCCA AAAAAAATCA CAAAATTATG 
GATGGCTACG AAGGAGGTCA GGAAAACGAA GATAATGATG AAGACTCAGA AGATAGTGGC 
TCGCATAAAA ATAAGAGAAA GGAAGGTAAT AGTTCTTTAT CGGCAACACT GAACACTTGG 
CTTTCAAAAT TTTCAAGTAC TTCTGAAGAT TCACAGAAGA AGAAGGAACA GGCAAATGAA 
TTAGGCAACG ATTTTGAATA TGATGAAGAT GTGGCTGATT TTGCAGAAAT TTTGCCGAAG 
CAGTCATCTA GTAATATTGA AAAAATTTTT AAGCATGGTA ACAATTCAGG ATCAATGGTA 
TCATATAATT CAAGTATCAA GCTCAATAGG AGGGAGAATA TATTGATAGG TCCACCAGAG 
TTGAGATTTT CTAATGAAAT CAAGGAACAC AATAGTATTG CTACAATATT CAAACTGGTA 
TTTATTCAAA CCAATAGAAG AGTCGTCGAA AAAATAGATT TGGCGAACAT GAAATGGGGG 
ACAATCCACG GAGGCAGTAA GTACATGAAG CCTTTACCCG TCCCGAAAGA TTTGGTTGCG 
AGTGTTGCTA AGAACGAAAG TGAGACGAGA AACTTAGCTA CCTTATGCGG TAATGGTCTA 
GATTTTGAGA TACCGGTGCC TGATTTTAAT ATTTTTGGCA AATGTATGGA AGATGAGCAA 
GATGTAGCCA AAATTGGTAA TCAGAATGTG GAAGATTTGA AAGTTACTGG GGGGCGGGAG 
GTGACAATTT GGAAGCAGAT CCAAGATATG AAGTTGAGAA CAAGGATACA AAAAATCTGT 
GTTTTAATTG AGACGTTCAA TGCTACAGTA AGAGAATATT TTGAGTTTTC CAATCGATTA 
GAGCATGACG GTATATTTAT AGATTTTGAA GTGAGGAAAC CTAGTAGTAA TAATTCTATT 
AATATCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010210.1
CDS	1..2955
Translation	MGPAIHNQLYECGLKTTSQDGFLMDNYETQLFQSLNRFINFINNANQSASNKEQNTKFCKSSSNFLRLKLLTVLRQWCDSSRSNCTLEVRDVLTQWWVTLLNFLNSDTSLQIDTALELSLSIELTSVCLECLSKIMTILIILPFHSSRDMEIYSHHLLLTIHCITNKLILISKNSKKLKRTNSDDKCSINDKKLQYLNKYSSLLRAFIGKLNAYAFFYLPEDFHFDTILLLTVSPQISSSIQTSLFSWKKRQYKFTDDQGQMIRTEAFENKDTKFFKIIVSYIKNDFVLMSFYWHYWYIILQFMKFSDSDVGIKKSTLSCIPGSEILLTHVTTRFLNSDLNKFTRIIKQTPNPRIANENVTESHPNFKSLNSSNALITSERINDYVFSNFKTIKLWECLRSLSGCILKENHPEYLENMLSLHESLLIDYVSTISAYDYIAANVIYNKVLQFIIFQFESLPSLKFIQWRSWYNGLLSMLRTKNVNCQTVSLLCLFNIWKHVTIEDRDEIVKVLLSDFWESLIFENEFPLIRILFMKVLVFKIIPSVQNSSSLRFLPHDRIKQLYEELLVNKEELFEMQKHDSNDIVAHRKNALVFNGNSRLMMIPKKPNTEDHLVYKINHDKNLTTERFPSVSSVANTRPNVILKNGKYAYDILDEMTSKAAFLLAEKKTRLNPKKNHKIMDGYEGGQENEDNDEDSEDSGSHKNKRKEGNSSLSATLNTWLSKFSSTSEDSQKKKEQANELGNDFEYDEDVADFAEILPKQSSSNIEKIFKHGNNSGSMVSYNSSIKLNRRENILIGPPELRFSNEIKEHNSIATIFKLVFIQTNRRVVEKIDLANMKWGTIHGGSKYMKPLPVPKDLVASVAKNESETRNLATLCGNGLDFEIPVPDFNIFGKCMEDEQDVAKIGNQNVEDLKVTGGREVTIWKQIQDMKLRTRIQKICVLIETFNATVREYFEFSNRLEHDGIFIDFEVRKPSSNNSINIKV

Target ORF information:

RefSeq Version	NM_001180132.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ahk1p (AHK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180132.1

ORF Insert Sequence:

ATGGGTCCTG CCATACATAA TCAACTATAT GAATGTGGAT TAAAGACTAC GTCTCAAGAT 
GGCTTCTTGA TGGACAATTA TGAAACTCAA TTATTTCAAT CGTTGAACAG GTTTATCAAT 
TTCATAAACA ATGCTAATCA GTCAGCTAGT AATAAAGAAC AAAATACAAA ATTTTGCAAA 
TCCTCCTCAA ATTTTTTACG CTTGAAACTA CTAACGGTTC TAAGACAATG GTGTGATTCT 
TCACGAAGCA ACTGTACACT GGAGGTTAGA GATGTTTTGA CCCAATGGTG GGTCACACTT 
TTAAACTTTC TAAATTCTGA CACCTCGCTT CAAATAGATA CTGCCCTTGA ATTGAGCTTG 
AGCATAGAAC TAACTTCCGT GTGTCTCGAG TGCCTCAGCA AAATTATGAC CATACTAATA 
ATATTGCCTT TCCATTCATC CAGAGACATG GAAATATACT CACATCATCT TTTGCTGACC 
ATTCATTGCA TAACAAACAA GTTGATTTTG ATTTCTAAAA ACTCCAAAAA ACTTAAACGT 
ACAAATAGTG ATGACAAGTG CTCTATAAAC GACAAGAAGC TGCAATACCT TAACAAATAT 
TCTTCCTTGC TGCGAGCATT CATCGGGAAG TTAAATGCTT ACGCATTTTT TTATCTACCA 
GAAGACTTTC ATTTCGATAC AATTTTGTTA TTGACAGTTT CTCCTCAAAT CTCTTCAAGT 
ATTCAAACAT CTTTGTTTTC ATGGAAGAAG AGACAATATA AATTCACCGA CGATCAGGGA 
CAAATGATCC GCACTGAAGC TTTTGAAAAT AAAGATACTA AATTTTTTAA GATCATTGTT 
TCTTATATTA AAAATGATTT TGTACTTATG TCATTTTATT GGCACTATTG GTATATCATA 
CTTCAATTTA TGAAATTCTC TGATTCAGAT GTAGGAATTA AAAAATCGAC ATTGTCATGC 
ATACCTGGCT CAGAAATTTT ATTGACGCAT GTAACCACTA GATTCTTGAA TTCAGATTTG 
AATAAATTCA CGCGGATTAT TAAACAAACG CCGAATCCAA GGATAGCAAA TGAGAATGTG 
ACAGAGTCAC ATCCAAATTT CAAGAGTTTG AATAGTAGCA ATGCTTTAAT TACATCTGAA 
AGGATTAATG ATTACGTATT TTCTAATTTT AAGACAATCA AATTATGGGA ATGTCTTAGA 
AGCCTTTCAG GATGCATTCT TAAAGAAAAT CATCCGGAAT ACCTGGAAAA TATGCTTTCC 
TTACATGAGT CTCTATTGAT AGACTATGTC TCCACTATTT CAGCGTACGA TTATATTGCA 
GCGAACGTCA TTTACAATAA GGTTCTCCAG TTTATTATTT TCCAGTTTGA AAGTTTACCT 
TCTCTGAAAT TCATTCAATG GAGGTCGTGG TACAATGGGC TTTTGAGTAT GTTAAGGACC 
AAAAATGTTA ACTGCCAGAC TGTTTCTTTA TTATGCCTTT TCAATATTTG GAAGCACGTA 
ACCATTGAGG ACCGCGATGA AATAGTAAAA GTGCTGTTAA GTGATTTTTG GGAGTCATTG 
ATTTTTGAAA ACGAATTTCC TTTGATTAGA ATTTTATTTA TGAAGGTATT AGTTTTTAAA 
ATCATTCCAA GTGTTCAAAA TTCTTCTTCA TTACGATTCT TGCCACATGA TAGAATTAAA 
CAACTATATG AAGAGCTACT GGTAAACAAG GAGGAGTTAT TTGAGATGCA AAAACACGAT 
TCAAATGATA TAGTGGCGCA TAGAAAGAAT GCTCTCGTTT TTAATGGTAA CTCACGTTTG 
ATGATGATTC CAAAAAAACC AAATACAGAG GACCACTTAG TTTATAAGAT TAATCATGAT 
AAAAATTTAA CTACAGAAAG ATTTCCCTCT GTCAGCTCAG TGGCAAATAC AAGACCTAAC 
GTTATCTTGA AAAATGGTAA ATACGCTTAT GATATTTTGG ACGAAATGAC AAGTAAAGCT 
GCCTTTCTTC TTGCAGAAAA GAAGACCCGA CTAAATCCCA AAAAAAATCA CAAAATTATG 
GATGGCTACG AAGGAGGTCA GGAAAACGAA GATAATGATG AAGACTCAGA AGATAGTGGC 
TCGCATAAAA ATAAGAGAAA GGAAGGTAAT AGTTCTTTAT CGGCAACACT GAACACTTGG 
CTTTCAAAAT TTTCAAGTAC TTCTGAAGAT TCACAGAAGA AGAAGGAACA GGCAAATGAA 
TTAGGCAACG ATTTTGAATA TGATGAAGAT GTGGCTGATT TTGCAGAAAT TTTGCCGAAG 
CAGTCATCTA GTAATATTGA AAAAATTTTT AAGCATGGTA ACAATTCAGG ATCAATGGTA 
TCATATAATT CAAGTATCAA GCTCAATAGG AGGGAGAATA TATTGATAGG TCCACCAGAG 
TTGAGATTTT CTAATGAAAT CAAGGAACAC AATAGTATTG CTACAATATT CAAACTGGTA 
TTTATTCAAA CCAATAGAAG AGTCGTCGAA AAAATAGATT TGGCGAACAT GAAATGGGGG 
ACAATCCACG GAGGCAGTAA GTACATGAAG CCTTTACCCG TCCCGAAAGA TTTGGTTGCG 
AGTGTTGCTA AGAACGAAAG TGAGACGAGA AACTTAGCTA CCTTATGCGG TAATGGTCTA 
GATTTTGAGA TACCGGTGCC TGATTTTAAT ATTTTTGGCA AATGTATGGA AGATGAGCAA 
GATGTAGCCA AAATTGGTAA TCAGAATGTG GAAGATTTGA AAGTTACTGG GGGGCGGGAG 
GTGACAATTT GGAAGCAGAT CCAAGATATG AAGTTGAGAA CAAGGATACA AAAAATCTGT 
GTTTTAATTG AGACGTTCAA TGCTACAGTA AGAGAATATT TTGAGTTTTC CAATCGATTA 
GAGCATGACG GTATATTTAT AGATTTTGAA GTGAGGAAAC CTAGTAGTAA TAATTCTATT 
AATATCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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AHK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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