PUF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PUF3
Entrez Gene ID	850647
Full Name	mRNA-binding protein PUF3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181833.1	NP_013088.1	mRNA-binding protein PUF3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

multi-omics data point to multiple classes of Puf3p targets, which display coherent post-transcriptional regulatory properties and suggest Puf3p plays a broad, but nuanced, role in the fine-tuning of gene expression
Title: Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.

The results indicate a likely role Puf3p in the control of translation under oxidative stress conditions, linking its role in repression of protein synthesis to mitochondrially active genes.
Title: Puf3p induces translational repression of genes linked to oxidative stress.

Data show that Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration that does not involve changes in overall mitochondrial biogenesis and largely independent of TORC1-mitochondrial signaling.
Title: Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

although the Puf3p regulon is conserved among all of the studied fungi, the dedicated regulation of mitochondrial biogenesis by Puf3p emerged only in the Saccharomycotina clade
Title: Tinkering evolution of post-transcriptional RNA regulons: puf3p in fungi as an example.

the PAB1-mRNP structure is critical for PUF3 action, and multiple components of the CCR4-NOT deadenylase complex, but not PAN2, interacted with PUF3
Title: PUF3 acceleration of deadenylation in vivo can operate independently of CCR4 activity, possibly involving effects on the PAB1-mRNP structure.

The following PUF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00317	NM_001181833.1 Latest version!	Saccharomyces cerevisiae S288C mRNA-binding protein PUF3 (PUF3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUF3 ( NM_001181833.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013088.1 Saccharomyces cerevisiae S288C mRNA-binding protein PUF3 (PUF3), partial mRNA.

Price & Availability↑

CloneID	OSi00317
Clone ID Related Accession (Same CDS sequence)	NM_001181833.1
Accession Version	NM_001181833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mRNA-binding protein PUF3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAATGA ACATGGATAT GGATATGGAT ATGGAACTCG CATCCATAGT TTCTTCTTTA 
AGCGCCCTGT CCCATAGTAA CAACAACGGC GGTCAGGCCG CCGCGGCCGG CATTGTGAAC 
GGCGGTGCTG CTGGATCACA GCAAATTGGC GGCTTCAGAA GGTCGAGTTT CACCACGGCA 
AATGAAGTAG ATTCAGAGAT CTTGCTGCTT CACGGTTCCA GTGAATCTTC ACCGATCTTC 
AAGAAAACCG CCCTATCAGT TGGTACTGCT CCGCCATTCT CCACGAACTC GAAAAAGTTT 
TTTGGTAACG GCGGCAATTA TTACCAGTAC AGATCAACCG ACACTGCCTC CCTGTCATCC 
GCATCGTACA ATAACTATCA TACTCATCAT ACTGCGGCTA ATCTCGGGAA AAACAACAAG 
GTCAACCATC TTCTGGGCCA GTATTCAGCT TCCATTGCTG GTCCCGTTTA CTACAATGGC 
AATGACAACA ACAACTCTGG TGGGGAAGGG TTTTTTGAGA AGTTCGGAAA ATCTTTGATT 
GACGGTACAA GGGAACTGGA ATCTCAAGAT AGACCCGACG CTGTCAATAC TCAGTCCCAA 
TTCATATCAA AAAGCGTCTC TAACGCCAGC TTAGACACAC AGAATACTTT CGAGCAAAAT 
GTCGAGTCTG ACAAAAACTT CAACAAATTG AATAGGAACA CTACTAACAG TGGTTCGCTT 
TACCACTCCA GTTCAAATTC AGGCTCATCT GCGTCTTTAG AGTCCGAAAA TGCTCATTAC 
CCCAAGAGAA ATATCTGGAA CGTAGCCAAC ACACCAGTAT TTAGACCTAG TAACAATCCA 
GCCGCTGTTG GGGCCACAAA TGTCGCGCTT CCTAACCAAC AAGATGGGCC TGCAAATAAC 
AATTTCCCCC CCTACATGAA CGGCTTCCCA CCCAATCAGT TTCATCAGGG CCCTCATTAC 
CAAAACTTCC CCAACTACTT GATCGGATCT CCTTCAAATT TCATCTCCCA GATGATTTCA 
GTACAGATTC CAGCAAATGA AGACACCGAA GACTCAAATG GTAAGAAAAA GAAGAAGGCA 
AACAGACCTT CTTCTGTTTC TTCACCTTCG TCCCCTCCAA ACAATTCTCC GTTCCCGTTT 
GCCTACCCCA ACCCAATGAT GTTTATGCCA CCTCCACCAC TCTCTGCTCC CCAGCAACAA 
CAGCAACAGC AACAACAACA ACAACAAGAA GACCAACAAC AGCAACAGCA ACAGGAGAAT 
CCTTATATTT ACTACCCTAC TCCAAATCCT ATACCTGTCA AAATGCCCAA GGACGAAAAA 
ACCTTCAAGA AAAGAAATAA CAAGAATCAT CCTGCAAATA ACTCCAACAA CGCCAACAAA 
CAGGCAAATC CTTATCTAGA AAATTCTATA CCCACAAAGA ATACCTCTAA AAAGAATGCT 
TCTTCCAAAT CTAATGAGTC AACGGCCAAC AACCATAAAT CTCACTCTCA TTCTCATCCT 
CATTCTCAAA GTCTACAGCA ACAGCAGCAA ACGTATCATC GATCGCCGTT GTTAGAGCAA 
CTAAGAAACA GTAGTTCTGA CAAAAATTCA AACTCGAACA TGTCACTAAA AGACATCTTT 
GGCCATTCCT TAGAGTTTTG CAAAGATCAA CATGGTTCAC GGTTCATTCA GCGCGAGTTG 
GCCACCTCAC CAGCCTCAGA AAAGGAGGTC ATATTCAATG AAATTCGCGA TGACGCCATT 
GAGCTTTCGA ACGATGTATT TGGTAATTAC GTCATTCAGA AGTTTTTTGA ATTTGGTAGC 
AAAATTCAAA AAAATACTTT GGTTGATCAA TTTAAAGGTA ACATGAAACA GTTATCTCTA 
CAAATGTACG CATGTCGTGT AATTCAAAAG GCGTTAGAAT ATATTGATTC CAATCAAAGA 
ATTGAGTTAG TCCTGGAATT ATCTGACTCC GTTTTGCAAA TGATCAAAGA TCAGAACGGT 
AATCACGTAA TTCAAAAGGC CATCGAAACA ATTCCTATCG AAAAATTACC TTTTATCTTA 
AGTTCTTTAA CTGGCCACAT TTACCATCTA TCTACCCACT CCTACGGCTG TAGGGTCATT 
CAAAGATTGT TAGAATTTGG TTCGAGCGAA GATCAGGAAA GCATTTTAAA CGAATTGAAA 
GATTTCATAC CATATCTAAT TCAAGACCAA TATGGTAACT ATGTCATTCA ATATGTTTTG 
CAACAAGATC AGTTCACCAA TAAGGAAATG GTCGATATCA AACAGGAAAT CATAGAAACT 
GTGGCCAATA ATGTTGTCGA ATACTCTAAA CATAAATTTG CCTCCAACGT GGTCGAAAAA 
TCTATTCTTT ATGGCTCTAA AAATCAGAAG GATTTAATAA TTTCTAAGAT TTTACCAAGA 
GACAAAAATC ATGCTTTGAA TCTGGAAGAC GATTCTCCAA TGATTTTAAT GATCAAGGAT 
CAATTTGCCA ACTATGTGAT TCAAAAATTA GTTAATGTCT CTGAGGGTGA GGGTAAGAAA 
TTGATCGTCA TTGCCATTAG AGCATATTTG GATAAACTAA ACAAATCTAA CTCCCTAGGA 
AATAGACATT TAGCCAGTGT TGAGAAACTT GCAGCATTGG TTGAAAATGC GGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013088.1
CDS	1..2640
Translation	MEMNMDMDMDMELASIVSSLSALSHSNNNGGQAAAAGIVNGGAAGSQQIGGFRRSSFTTANEVDSEILLLHGSSESSPIFKKTALSVGTAPPFSTNSKKFFGNGGNYYQYRSTDTASLSSASYNNYHTHHTAANLGKNNKVNHLLGQYSASIAGPVYYNGNDNNNSGGEGFFEKFGKSLIDGTRELESQDRPDAVNTQSQFISKSVSNASLDTQNTFEQNVESDKNFNKLNRNTTNSGSLYHSSSNSGSSASLESENAHYPKRNIWNVANTPVFRPSNNPAAVGATNVALPNQQDGPANNNFPPYMNGFPPNQFHQGPHYQNFPNYLIGSPSNFISQMISVQIPANEDTEDSNGKKKKKANRPSSVSSPSSPPNNSPFPFAYPNPMMFMPPPPLSAPQQQQQQQQQQQQEDQQQQQQQENPYIYYPTPNPIPVKMPKDEKTFKKRNNKNHPANNSNNANKQANPYLENSIPTKNTSKKNASSKSNESTANNHKSHSHSHPHSQSLQQQQQTYHRSPLLEQLRNSSSDKNSNSNMSLKDIFGHSLEFCKDQHGSRFIQRELATSPASEKEVIFNEIRDDAIELSNDVFGNYVIQKFFEFGSKIQKNTLVDQFKGNMKQLSLQMYACRVIQKALEYIDSNQRIELVLELSDSVLQMIKDQNGNHVIQKAIETIPIEKLPFILSSLTGHIYHLSTHSYGCRVIQRLLEFGSSEDQESILNELKDFIPYLIQDQYGNYVIQYVLQQDQFTNKEMVDIKQEIIETVANNVVEYSKHKFASNVVEKSILYGSKNQKDLIISKILPRDKNHALNLEDDSPMILMIKDQFANYVIQKLVNVSEGEGKKLIVIAIRAYLDKLNKSNSLGNRHLASVEKLAALVENAEV

Target ORF information:

RefSeq Version	NM_001181833.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mRNA-binding protein PUF3 (PUF3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181833.1

ORF Insert Sequence:

ATGGAAATGA ACATGGATAT GGATATGGAT ATGGAACTCG CATCCATAGT TTCTTCTTTA 
AGCGCCCTGT CCCATAGTAA CAACAACGGC GGTCAGGCCG CCGCGGCCGG CATTGTGAAC 
GGCGGTGCTG CTGGATCACA GCAAATTGGC GGCTTCAGAA GGTCGAGTTT CACCACGGCA 
AATGAAGTAG ATTCAGAGAT CTTGCTGCTT CACGGTTCCA GTGAATCTTC ACCGATCTTC 
AAGAAAACCG CCCTATCAGT TGGTACTGCT CCGCCATTCT CCACGAACTC GAAAAAGTTT 
TTTGGTAACG GCGGCAATTA TTACCAGTAC AGATCAACCG ACACTGCCTC CCTGTCATCC 
GCATCGTACA ATAACTATCA TACTCATCAT ACTGCGGCTA ATCTCGGGAA AAACAACAAG 
GTCAACCATC TTCTGGGCCA GTATTCAGCT TCCATTGCTG GTCCCGTTTA CTACAATGGC 
AATGACAACA ACAACTCTGG TGGGGAAGGG TTTTTTGAGA AGTTCGGAAA ATCTTTGATT 
GACGGTACAA GGGAACTGGA ATCTCAAGAT AGACCCGACG CTGTCAATAC TCAGTCCCAA 
TTCATATCAA AAAGCGTCTC TAACGCCAGC TTAGACACAC AGAATACTTT CGAGCAAAAT 
GTCGAGTCTG ACAAAAACTT CAACAAATTG AATAGGAACA CTACTAACAG TGGTTCGCTT 
TACCACTCCA GTTCAAATTC AGGCTCATCT GCGTCTTTAG AGTCCGAAAA TGCTCATTAC 
CCCAAGAGAA ATATCTGGAA CGTAGCCAAC ACACCAGTAT TTAGACCTAG TAACAATCCA 
GCCGCTGTTG GGGCCACAAA TGTCGCGCTT CCTAACCAAC AAGATGGGCC TGCAAATAAC 
AATTTCCCCC CCTACATGAA CGGCTTCCCA CCCAATCAGT TTCATCAGGG CCCTCATTAC 
CAAAACTTCC CCAACTACTT GATCGGATCT CCTTCAAATT TCATCTCCCA GATGATTTCA 
GTACAGATTC CAGCAAATGA AGACACCGAA GACTCAAATG GTAAGAAAAA GAAGAAGGCA 
AACAGACCTT CTTCTGTTTC TTCACCTTCG TCCCCTCCAA ACAATTCTCC GTTCCCGTTT 
GCCTACCCCA ACCCAATGAT GTTTATGCCA CCTCCACCAC TCTCTGCTCC CCAGCAACAA 
CAGCAACAGC AACAACAACA ACAACAAGAA GACCAACAAC AGCAACAGCA ACAGGAGAAT 
CCTTATATTT ACTACCCTAC TCCAAATCCT ATACCTGTCA AAATGCCCAA GGACGAAAAA 
ACCTTCAAGA AAAGAAATAA CAAGAATCAT CCTGCAAATA ACTCCAACAA CGCCAACAAA 
CAGGCAAATC CTTATCTAGA AAATTCTATA CCCACAAAGA ATACCTCTAA AAAGAATGCT 
TCTTCCAAAT CTAATGAGTC AACGGCCAAC AACCATAAAT CTCACTCTCA TTCTCATCCT 
CATTCTCAAA GTCTACAGCA ACAGCAGCAA ACGTATCATC GATCGCCGTT GTTAGAGCAA 
CTAAGAAACA GTAGTTCTGA CAAAAATTCA AACTCGAACA TGTCACTAAA AGACATCTTT 
GGCCATTCCT TAGAGTTTTG CAAAGATCAA CATGGTTCAC GGTTCATTCA GCGCGAGTTG 
GCCACCTCAC CAGCCTCAGA AAAGGAGGTC ATATTCAATG AAATTCGCGA TGACGCCATT 
GAGCTTTCGA ACGATGTATT TGGTAATTAC GTCATTCAGA AGTTTTTTGA ATTTGGTAGC 
AAAATTCAAA AAAATACTTT GGTTGATCAA TTTAAAGGTA ACATGAAACA GTTATCTCTA 
CAAATGTACG CATGTCGTGT AATTCAAAAG GCGTTAGAAT ATATTGATTC CAATCAAAGA 
ATTGAGTTAG TCCTGGAATT ATCTGACTCC GTTTTGCAAA TGATCAAAGA TCAGAACGGT 
AATCACGTAA TTCAAAAGGC CATCGAAACA ATTCCTATCG AAAAATTACC TTTTATCTTA 
AGTTCTTTAA CTGGCCACAT TTACCATCTA TCTACCCACT CCTACGGCTG TAGGGTCATT 
CAAAGATTGT TAGAATTTGG TTCGAGCGAA GATCAGGAAA GCATTTTAAA CGAATTGAAA 
GATTTCATAC CATATCTAAT TCAAGACCAA TATGGTAACT ATGTCATTCA ATATGTTTTG 
CAACAAGATC AGTTCACCAA TAAGGAAATG GTCGATATCA AACAGGAAAT CATAGAAACT 
GTGGCCAATA ATGTTGTCGA ATACTCTAAA CATAAATTTG CCTCCAACGT GGTCGAAAAA 
TCTATTCTTT ATGGCTCTAA AAATCAGAAG GATTTAATAA TTTCTAAGAT TTTACCAAGA 
GACAAAAATC ATGCTTTGAA TCTGGAAGAC GATTCTCCAA TGATTTTAAT GATCAAGGAT 
CAATTTGCCA ACTATGTGAT TCAAAAATTA GTTAATGTCT CTGAGGGTGA GGGTAAGAAA 
TTGATCGTCA TTGCCATTAG AGCATATTTG GATAAACTAA ACAAATCTAA CTCCCTAGGA 
AATAGACATT TAGCCAGTGT TGAGAAACTT GCAGCATTGG TTGAAAATGC GGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PUF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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